cacna1g gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_017358537.2	XP_017214026.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_021480347.1	XP_021336022.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
XM_017358536.1	XP_017214025.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_017358537.1	XP_017214026.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
XM_021480344.1	XP_021336019.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_021480346.1	XP_021336021.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
XM_017358536.2	XP_017214025.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
XM_021480348.1	XP_021336023.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
XM_021480349.1	XP_021336024.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
XM_017358535.1	XP_017214024.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
XM_021480345.1	XP_021336020.1	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
XM_017358535.2	XP_017214024.2	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54517	XM_017358535.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa54518	XM_017358536.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148360	XM_021480344.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148361	XM_021480345.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148362	XM_017358537.2 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148363	XM_021480346.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148364	XM_021480347.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148365	XM_017358535.2 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148366	XM_017358536.2 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148367	XM_021480348.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa148368	XM_021480349.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa54519	XM_017358537.1 Latest version!	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

cacna1g ( XM_017358535.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214024.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54517
Clone ID Related Accession (Same CDS sequence)	XM_017358535.1
Accession Version	XM_017358535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT 
GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG 
CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA 
GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA 
GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC 
AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC 
TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT 
GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA 
GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG 
GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC 
CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG 
CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT 
TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG 
GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC 
TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC 
ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC 
GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC 
AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC 
TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG 
GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC 
CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC 
AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA 
AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT 
TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA 
GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA 
CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC 
GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG 
CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC 
GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA 
CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAATAT AATGCTGACC 
GGTGTCAGTT GGTCCAGTCC TGATCACACA CAAGAAGCCC AGAGTAAACC CTTTTACTCC 
GATTACTGCC CAACACGACG TCTCATCTAC AACATGTGCA AGAGCCAGTA TCTGGACCTG 
TTCATCACCA TCGTCATCGC CCTCAATGTC ATCACCATGT CTATGGAGCA CTACCACCAA 
CCAAAGGTTT TGAGCGATTC CCTAAAGATC TGTAACTACA TCTTCACAGT CATATTTGTG 
CTGGAGTCCA TCTTCAAACT TGTTGCTTTT GGATTTCGCC GCTTCTTCAA GGACAGATGG 
AACCAGTTGG ATCTGGCCAT TGTTTTGTTG TCTATCATGG GCATAACACT GGAGGAAATC 
GAGGTCAACG CTTCCCTTCC CATCAATCCT ACCATCATTC GCATCATGAG GGTGTTGCGC 
ATCACTCGAG TTCTAAAGCT ACTGAAGATG GCGGTGGGAA TGCGAGCTCT ACTAGACACT 
GTGATGCAGG CCCTTCCTCA GGTGGGGAAT CTGGGTCTTC TCTTCATGTT GCTTTTCTTC 
ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT GGTGACCTGA TATGTGATGA GCTACACCCC 
TGTGAAGGTC TTGGCCGTTA TGCCACGTTC AAAAATTTTG GCATGGCTTT CCTCCTGCTG 
TTCCGTGTAT CCACCGGGGA CAACTGGAAT GGCATCATGA AGGATACGCT CAGAGACTGC 
TCCCAAGACA CCGGTATATG CTACAACACG GTGGTGTCCC CCATTTACTT TGTGTCCTTT 
GTGCTGACAG CTCAGTTTGT GCTGGTCAAT GTGGTGATCG CTGTGCTCAT GAAGCACCTA 
GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG 
GAGATGGAAG CTTTGGGTAG AGATAGAGAA ATGCAGAGAG GGCACATGCC ACGTTTGGGT 
TCTGGCGATT TGGGTGGGCC AGGAGGGTCA CCTTGGATAT CAAGAGACCA TCGAGACCTG 
TCAGGACCAC TTTACCCCAT AGACAGCCCA ACAGCAGACA TACGCAGAGA TTTTGAAGAT 
CATTTAAGGA CAGATCCTGA TCCACCTCCG AATCTGGAGC CACCATTAGA GCGGAGGCCT 
ATGTTTGACT CAGTTTCCCT GGTGATCCAG GGCTCGCTGG AAGGAGAGCT CAGCCTCATG 
GACAACCTGT CTGGCTCCAT CTGCCACTAC TACGCTCTGC CCCCCCTTCC CAACAAACAC 
TGCACTGAGA AACAGATTCC TCTAGCCGAG ATGGAGGCTC TATCTCTGGC ATCGGAGAAA 
TCCTGGTCCT TAGCTCTGAC GGATGACTCT GTCCCAGATG ATTTTAACCC CCTCTTACTA 
ACCAGTCAGG AGTGCAATAC CACAGATCCT TCAGATCCAC CAGACACCCG GCAGGCGGAT 
GAACCGACGG ATGAACACTT GCTATCCGTG AAGAAGACCT CAGTGGGCCG CACACATTCT 
CTACCTAATG ACAGCTACAT GTTCCTGCCG CTGCAGTCCA ACTCAAACCA CAGCACCCAC 
ACAACCTCAC CTCACCTCGC TCAGACAGGG TCTACAGCTT CTGTTCAGTC TCAAACAGAA 
GAAGCCAGCC AGCACTTGAC GGTGCCCAGC GATCTGTTCA GACCCATCAG TCCTCACAGT 
TTATCAGACT CAGAGAGCAT CCCTCGAATT CCCCCACCTC GGCGGGCACA CACTCTCTCA 
CGCACACTCC GCAGACAGGT GGCAGTGAGT GCTGACTCTC AGGAGACGCT TTACACAGAG 
GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC AGGGAACTGT CGGACCCCCT TCCACCTCAG 
CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG GTCCCTGCCA CACCAGGCGC CTCCCCAAAA 
CCTTCACGAC CTAGCGTCCA CACGCAGCAC AACCCCTACG ACCAGTACAA TGTGTTCTCC 
AGGAGTTCCC ACTCTCCACC TGTACCACCA CCACCACCAG ACTATAAGAA GCAGGAGGAT 
GTTGACTCGG TGGATCAGGA GGTATCTCGA ATTATCCGAG CGGGACTCAC AGGGAGAAGC 
GATGATGTCA ACCGAGAAGG GACAGGAGAT CAATGTGCAA AACGTCAGCT TAAGAAATAC 
CATAGCGTGG ACACTCAAGG CCAGCGAGCT CTGCTGTTAC CGCGGCCCTT GTCATGGCTT 
GATGACCCAC GCCGGCACTC CATCGAAGTA TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC 
AGTTCCATAT CGTCAGGGTT TGTATCACGA GCCGACAGCC TCCAGCAACA ATCTTCGCCC 
CGTGGACGCA AGAAGAAGAT GAGTCCACCG TGCATTTCTG TAGATCCACC CGAGGATCCG 
GAACTTCCGG GAGGATTCCA TCCTGCTCTG GGGATTGTGC CACCTCCATT GCCAAGCCGG 
GACACTTGCC TGAGGAGGCG CGCTCCCTCC AGTGAATCCA AGGACTCGTT TGATTTGGGA 
GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT CCAAACTCCA AGCTATTGAC TCTGCCTAGC 
TTCTCTTTCG AGAAAACAAG CTCTGAGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214024.1
CDS	12..4664
Translation	MHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358535.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358535.1

ORF Insert Sequence:

ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT 
GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG 
CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA 
GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA 
GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC 
AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC 
TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT 
GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA 
GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG 
GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC 
CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG 
CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT 
TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG 
GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC 
TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC 
ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC 
GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC 
AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC 
TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG 
GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC 
CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC 
AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA 
AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT 
TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA 
GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA 
CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC 
GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG 
CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC 
GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA 
CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAATAT AATGCTGACC 
GGTGTCAGTT GGTCCAGTCC TGATCACACA CAAGAAGCCC AGAGTAAACC CTTTTACTCC 
GATTACTGCC CAACACGACG TCTCATCTAC AACATGTGCA AGAGCCAGTA TCTGGACCTG 
TTCATCACCA TCGTCATCGC CCTCAATGTC ATCACCATGT CTATGGAGCA CTACCACCAA 
CCAAAGGTTT TGAGCGATTC CCTAAAGATC TGTAACTACA TCTTCACAGT CATATTTGTG 
CTGGAGTCCA TCTTCAAACT TGTTGCTTTT GGATTTCGCC GCTTCTTCAA GGACAGATGG 
AACCAGTTGG ATCTGGCCAT TGTTTTGTTG TCTATCATGG GCATAACACT GGAGGAAATC 
GAGGTCAACG CTTCCCTTCC CATCAATCCT ACCATCATTC GCATCATGAG GGTGTTGCGC 
ATCACTCGAG TTCTAAAGCT ACTGAAGATG GCGGTGGGAA TGCGAGCTCT ACTAGACACT 
GTGATGCAGG CCCTTCCTCA GGTGGGGAAT CTGGGTCTTC TCTTCATGTT GCTTTTCTTC 
ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT GGTGACCTGA TATGTGATGA GCTACACCCC 
TGTGAAGGTC TTGGCCGTTA TGCCACGTTC AAAAATTTTG GCATGGCTTT CCTCCTGCTG 
TTCCGTGTAT CCACCGGGGA CAACTGGAAT GGCATCATGA AGGATACGCT CAGAGACTGC 
TCCCAAGACA CCGGTATATG CTACAACACG GTGGTGTCCC CCATTTACTT TGTGTCCTTT 
GTGCTGACAG CTCAGTTTGT GCTGGTCAAT GTGGTGATCG CTGTGCTCAT GAAGCACCTA 
GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG 
GAGATGGAAG CTTTGGGTAG AGATAGAGAA ATGCAGAGAG GGCACATGCC ACGTTTGGGT 
TCTGGCGATT TGGGTGGGCC AGGAGGGTCA CCTTGGATAT CAAGAGACCA TCGAGACCTG 
TCAGGACCAC TTTACCCCAT AGACAGCCCA ACAGCAGACA TACGCAGAGA TTTTGAAGAT 
CATTTAAGGA CAGATCCTGA TCCACCTCCG AATCTGGAGC CACCATTAGA GCGGAGGCCT 
ATGTTTGACT CAGTTTCCCT GGTGATCCAG GGCTCGCTGG AAGGAGAGCT CAGCCTCATG 
GACAACCTGT CTGGCTCCAT CTGCCACTAC TACGCTCTGC CCCCCCTTCC CAACAAACAC 
TGCACTGAGA AACAGATTCC TCTAGCCGAG ATGGAGGCTC TATCTCTGGC ATCGGAGAAA 
TCCTGGTCCT TAGCTCTGAC GGATGACTCT GTCCCAGATG ATTTTAACCC CCTCTTACTA 
ACCAGTCAGG AGTGCAATAC CACAGATCCT TCAGATCCAC CAGACACCCG GCAGGCGGAT 
GAACCGACGG ATGAACACTT GCTATCCGTG AAGAAGACCT CAGTGGGCCG CACACATTCT 
CTACCTAATG ACAGCTACAT GTTCCTGCCG CTGCAGTCCA ACTCAAACCA CAGCACCCAC 
ACAACCTCAC CTCACCTCGC TCAGACAGGG TCTACAGCTT CTGTTCAGTC TCAAACAGAA 
GAAGCCAGCC AGCACTTGAC GGTGCCCAGC GATCTGTTCA GACCCATCAG TCCTCACAGT 
TTATCAGACT CAGAGAGCAT CCCTCGAATT CCCCCACCTC GGCGGGCACA CACTCTCTCA 
CGCACACTCC GCAGACAGGT GGCAGTGAGT GCTGACTCTC AGGAGACGCT TTACACAGAG 
GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC AGGGAACTGT CGGACCCCCT TCCACCTCAG 
CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG GTCCCTGCCA CACCAGGCGC CTCCCCAAAA 
CCTTCACGAC CTAGCGTCCA CACGCAGCAC AACCCCTACG ACCAGTACAA TGTGTTCTCC 
AGGAGTTCCC ACTCTCCACC TGTACCACCA CCACCACCAG ACTATAAGAA GCAGGAGGAT 
GTTGACTCGG TGGATCAGGA GGTATCTCGA ATTATCCGAG CGGGACTCAC AGGGAGAAGC 
GATGATGTCA ACCGAGAAGG GACAGGAGAT CAATGTGCAA AACGTCAGCT TAAGAAATAC 
CATAGCGTGG ACACTCAAGG CCAGCGAGCT CTGCTGTTAC CGCGGCCCTT GTCATGGCTT 
GATGACCCAC GCCGGCACTC CATCGAAGTA TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC 
AGTTCCATAT CGTCAGGGTT TGTATCACGA GCCGACAGCC TCCAGCAACA ATCTTCGCCC 
CGTGGACGCA AGAAGAAGAT GAGTCCACCG TGCATTTCTG TAGATCCACC CGAGGATCCG 
GAACTTCCGG GAGGATTCCA TCCTGCTCTG GGGATTGTGC CACCTCCATT GCCAAGCCGG 
GACACTTGCC TGAGGAGGCG CGCTCCCTCC AGTGAATCCA AGGACTCGTT TGATTTGGGA 
GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT CCAAACTCCA AGCTATTGAC TCTGCCTAGC 
TTCTCTTTCG AGAAAACAAG CTCTGAGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_017358536.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214025.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54518
Clone ID Related Accession (Same CDS sequence)	XM_017358536.1
Accession Version	XM_017358536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT 
GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG 
CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA 
GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA 
GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC 
AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC 
TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT 
GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA 
GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG 
GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC 
CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG 
CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT 
TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG 
GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC 
TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC 
ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC 
GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC 
AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC 
TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG 
GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC 
CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC 
AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA 
AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT 
TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA 
GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA 
CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC 
GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG 
CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC 
GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA 
CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAGTAA GGAGAAGGAG 
CTAGCTGAAG CCCAGAGTAA ACCCTTTTAC TCCGATTACT GCCCAACACG ACGTCTCATC 
TACAACATGT GCAAGAGCCA GTATCTGGAC CTGTTCATCA CCATCGTCAT CGCCCTCAAT 
GTCATCACCA TGTCTATGGA GCACTACCAC CAACCAAAGG TTTTGAGCGA TTCCCTAAAG 
ATCTGTAACT ACATCTTCAC AGTCATATTT GTGCTGGAGT CCATCTTCAA ACTTGTTGCT 
TTTGGATTTC GCCGCTTCTT CAAGGACAGA TGGAACCAGT TGGATCTGGC CATTGTTTTG 
TTGTCTATCA TGGGCATAAC ACTGGAGGAA ATCGAGGTCA ACGCTTCCCT TCCCATCAAT 
CCTACCATCA TTCGCATCAT GAGGGTGTTG CGCATCACTC GAGTTCTAAA GCTACTGAAG 
ATGGCGGTGG GAATGCGAGC TCTACTAGAC ACTGTGATGC AGGCCCTTCC TCAGGTGGGG 
AATCTGGGTC TTCTCTTCAT GTTGCTTTTC TTCATCTTTG CTGCTCTGGG AGTAGAGCTG 
TTTGGTGACC TGATATGTGA TGAGCTACAC CCCTGTGAAG GTCTTGGCCG TTATGCCACG 
TTCAAAAATT TTGGCATGGC TTTCCTCCTG CTGTTCCGTG TATCCACCGG GGACAACTGG 
AATGGCATCA TGAAGGATAC GCTCAGAGAC TGCTCCCAAG ACACCGGTAT ATGCTACAAC 
ACGGTGGTGT CCCCCATTTA CTTTGTGTCC TTTGTGCTGA CAGCTCAGTT TGTGCTGGTC 
AATGTGGTGA TCGCTGTGCT CATGAAGCAC CTAGAGGAAA GCAACAAAGA GGCCAAGGAG 
GCTGAAGCAG AGGCCGAGCT GGAGCTGGAG CAGGAGATGG AAGCTTTGGG TAGAGATAGA 
GAAATGCAGA GAGGGCACAT GCCACGTTTG GGTTCTGGCG ATTTGGGTGG GCCAGGAGGG 
TCACCTTGGA TATCAAGAGA CCATCGAGAC CTGTCAGGAC CACTTTACCC CATAGACAGC 
CCAACAGCAG ACATACGCAG AGATTTTGAA GATCATTTAA GGACAGATCC TGATCCACCT 
CCGAATCTGG AGCCACCATT AGAGCGGAGG CCTATGTTTG ACTCAGTTTC CCTGGTGATC 
CAGGGCTCGC TGGAAGGAGA GCTCAGCCTC ATGGACAACC TGTCTGGCTC CATCTGCCAC 
TACTACGCTC TGCCCCCCCT TCCCAACAAA CACTGCACTG AGAAACAGAT TCCTCTAGCC 
GAGATGGAGG CTCTATCTCT GGCATCGGAG AAATCCTGGT CCTTAGCTCT GACGGATGAC 
TCTGTCCCAG ATGATTTTAA CCCCCTCTTA CTAACCAGTC AGGAGTGCAA TACCACAGAT 
CCTTCAGATC CACCAGACAC CCGGCAGGCG GATGAACCGA CGGATGAACA CTTGCTATCC 
GTGAAGAAGA CCTCAGTGGG CCGCACACAT TCTCTACCTA ATGACAGCTA CATGTTCCTG 
CCGCTGCAGT CCAACTCAAA CCACAGCACC CACACAACCT CACCTCACCT CGCTCAGACA 
GGGTCTACAG CTTCTGTTCA GTCTCAAACA GAAGAAGCCA GCCAGCACTT GACGGTGCCC 
AGCGATCTGT TCAGACCCAT CAGTCCTCAC AGTTTATCAG ACTCAGAGAG CATCCCTCGA 
ATTCCCCCAC CTCGGCGGGC ACACACTCTC TCACGCACAC TCCGCAGACA GGTGGCAGTG 
AGTGCTGACT CTCAGGAGAC GCTTTACACA GAGGGAGGGG AAGCTGAGGG TCCTCTGGGG 
CTCAGGGAAC TGTCGGACCC CCTTCCACCT CAGCAGCAGC AGCACCAGCC TTCCCTCTTT 
CTGGTCCCTG CCACACCAGG CGCCTCCCCA AAACCTTCAC GACCTAGCGT CCACACGCAG 
CACAACCCCT ACGACCAGTA CAATGTGTTC TCCAGGAGTT CCCACTCTCC ACCTGTACCA 
CCACCACCAC CAGACTATAA GAAGCAGGAG GATGTTGACT CGGTGGATCA GGAGGTATCT 
CGAATTATCC GAGCGGGACT CACAGGGAGA AGCGATGATG TCAACCGAGA AGGGACAGGA 
GATCAATGTG CAAAACGTCA GCTTAAGAAA TACCATAGCG TGGACACTCA AGGCCAGCGA 
GCTCTGCTGT TACCGCGGCC CTTGTCATGG CTTGATGACC CACGCCGGCA CTCCATCGAA 
GTATGCTCGT CTGTAGAAAG CAGTCCGCAG CGCAGTTCCA TATCGTCAGG GTTTGTATCA 
CGAGCCGACA GCCTCCAGCA ACAATCTTCG CCCCGTGGAC GCAAGAAGAA GATGAGTCCA 
CCGTGCATTT CTGTAGATCC ACCCGAGGAT CCGGAACTTC CGGGAGGATT CCATCCTGCT 
CTGGGGATTG TGCCACCTCC ATTGCCAAGC CGGGACACTT GCCTGAGGAG GCGCGCTCCC 
TCCAGTGAAT CCAAGGACTC GTTTGATTTG GGAGGAGGAG AGGCGCTGCC TCAAGAGGGC 
GTTCCAAACT CCAAGCTATT GACTCTGCCT AGCTTCTCTT TCGAGAAAAC AAGCTCTGAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214025.1
CDS	12..4637
Translation	MHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRSKEKELAEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358536.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358536.1

ORF Insert Sequence:

ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGGTGATGCC TCAAAGTCGG ACTCTGAAGC CGATATTTAT 
GCACGGAGCT TGGAGGATGT TTCGGGACAC AAGAAAGATA TGTCTGCATC ATCCGTTGTG 
CCCATCAATG GCCATGTGGA TCTGAAGAGC AGCCTGACAC CGCCTCTGAT CACCCACACA 
GCTGCCACAC CAATGCCCAT CCCCAAGATA TCAGGAGGAG GAGATTCAGC ACTGAGCCAA 
GAGTCCCGCA GAGGCAGCAG TGTCTCCATG GAGCCTAATG GCCAAGACAA ATCTCCATCC 
AGTGCACGGA GCTCCCCTAA TGCCCCCTTG AGCTCCGCCA GTAGCTGGAA CAGCAGACGC 
TCCAGCTGGA ACAGTCTAGG CCGAGCCCCC AGCCTCAAGA GGCAGAAACA CCAGTCTGGT 
GAAAGGCGCT CCTTGTTGTC TGGTGATGGC CAGTCCAGTT CTGATGAGGG GGATGCAGGA 
GAGAGCATGG GGGGCATGTC TGAGGGTGAT GATGCCTCCC TCGCCAGGAC GGACTCGCTG 
GGCCAGAGGC CTCGACATAG GCGCATGGAG TCCCTGGAGA CGCGCAGCTC ATTTGATCTC 
CATCCAGACA CCCTGCAGGT GCCGTACCCC CACCGCTCCG GCAGCATACA CAGCGCACGG 
CCACCCAACT TCCTCAGCAA CGGCAAGACT TCTCCATCTG CAGCCACCAC ACAGCTCTCT 
TTGGATGAAC ACCACAGTGA AGACGACAAC GCAGACGAGG AAGGAAACCT GAGTCGCAGG 
GCTCGTCTGT ACAGATGGCT GGAGCACAAG CAGCCCGAAT GGTGTCGGCA GAGGGTCACC 
TGGTCCCTTT ACCTCTTTCC TCCAGAGTCA CGGTTTCGCG TGACATGCAA TAAAATCATC 
ACCCACAAGA TGTTTGACCA TGTGGTTCTT GTCATCATCT TTCTCAACTG CATCACCATC 
GCCATGGAGA GGCCCAGAAT CGACCCCTCT AGCGCTGAAC GCATCTTCTT GACGTTGTCC 
AACTACATAT TTACTGCCAT CTTTGTGACC GAAATGACAA TAAAGGTGGT TGCTTTGGGC 
TTTTGTTTTG GAGAGAAGAC CTACCTGAAG AGCAGCTGGA ACATTCTGGA TGGCATGCTG 
GTGATGATCT CTGTGATTGA CATCCTCGTG TCCCTGATCT CCAACAGTGG CACCAAGATC 
CTGGGCATGT TACGTGTGCT GCGGTTACTG AGGACACTCA GGCCACTCAG AGTGATCAGC 
AGAGCTCCTG GACTGAAACT GGTTGTTGAG ACATTGATGT CGTCTCTTAA GCCTATCGGA 
AACATTGTGG TCATCTGCTG TGCCTTCTTC ATTATTTTTG GGATCTTGGG AGTTCAGCTT 
TTCAAGGGAA AGTTCTTTGT GTGTCATGGG GATGATACGA GAAACATCAC CAACAAGTCA 
GATTGCCTCC TGGCAAACTA CAAATGGGTC AGACACAAGT ACAACTTTGA TAACCTTGGA 
CAGGCCCTCA TGTCCCTTTT TGTTCTGGCT TCCAAAGACG GTTGGGTGGA TATCATGTAC 
GATGGACTGG ATGCTGTCGG TGTGGACCAG CAGCCCGTCA TGAATTACAA CCCATGGATG 
CTGCTCTATT TTATCTCCTT CTTGCTGATC GTGGCTTTCT TCGTGCTCAA CATGTTTGTC 
GGCGTGGTCG TCGAGAACTT TCACAAGTGC AGGAGAAACC AGGAGGAGGA AGAAGCCAAA 
CGGCGAGAAG CGAAGCGCCA GAAACGACGT GATAAAAAAC GAAGGAGTAA GGAGAAGGAG 
CTAGCTGAAG CCCAGAGTAA ACCCTTTTAC TCCGATTACT GCCCAACACG ACGTCTCATC 
TACAACATGT GCAAGAGCCA GTATCTGGAC CTGTTCATCA CCATCGTCAT CGCCCTCAAT 
GTCATCACCA TGTCTATGGA GCACTACCAC CAACCAAAGG TTTTGAGCGA TTCCCTAAAG 
ATCTGTAACT ACATCTTCAC AGTCATATTT GTGCTGGAGT CCATCTTCAA ACTTGTTGCT 
TTTGGATTTC GCCGCTTCTT CAAGGACAGA TGGAACCAGT TGGATCTGGC CATTGTTTTG 
TTGTCTATCA TGGGCATAAC ACTGGAGGAA ATCGAGGTCA ACGCTTCCCT TCCCATCAAT 
CCTACCATCA TTCGCATCAT GAGGGTGTTG CGCATCACTC GAGTTCTAAA GCTACTGAAG 
ATGGCGGTGG GAATGCGAGC TCTACTAGAC ACTGTGATGC AGGCCCTTCC TCAGGTGGGG 
AATCTGGGTC TTCTCTTCAT GTTGCTTTTC TTCATCTTTG CTGCTCTGGG AGTAGAGCTG 
TTTGGTGACC TGATATGTGA TGAGCTACAC CCCTGTGAAG GTCTTGGCCG TTATGCCACG 
TTCAAAAATT TTGGCATGGC TTTCCTCCTG CTGTTCCGTG TATCCACCGG GGACAACTGG 
AATGGCATCA TGAAGGATAC GCTCAGAGAC TGCTCCCAAG ACACCGGTAT ATGCTACAAC 
ACGGTGGTGT CCCCCATTTA CTTTGTGTCC TTTGTGCTGA CAGCTCAGTT TGTGCTGGTC 
AATGTGGTGA TCGCTGTGCT CATGAAGCAC CTAGAGGAAA GCAACAAAGA GGCCAAGGAG 
GCTGAAGCAG AGGCCGAGCT GGAGCTGGAG CAGGAGATGG AAGCTTTGGG TAGAGATAGA 
GAAATGCAGA GAGGGCACAT GCCACGTTTG GGTTCTGGCG ATTTGGGTGG GCCAGGAGGG 
TCACCTTGGA TATCAAGAGA CCATCGAGAC CTGTCAGGAC CACTTTACCC CATAGACAGC 
CCAACAGCAG ACATACGCAG AGATTTTGAA GATCATTTAA GGACAGATCC TGATCCACCT 
CCGAATCTGG AGCCACCATT AGAGCGGAGG CCTATGTTTG ACTCAGTTTC CCTGGTGATC 
CAGGGCTCGC TGGAAGGAGA GCTCAGCCTC ATGGACAACC TGTCTGGCTC CATCTGCCAC 
TACTACGCTC TGCCCCCCCT TCCCAACAAA CACTGCACTG AGAAACAGAT TCCTCTAGCC 
GAGATGGAGG CTCTATCTCT GGCATCGGAG AAATCCTGGT CCTTAGCTCT GACGGATGAC 
TCTGTCCCAG ATGATTTTAA CCCCCTCTTA CTAACCAGTC AGGAGTGCAA TACCACAGAT 
CCTTCAGATC CACCAGACAC CCGGCAGGCG GATGAACCGA CGGATGAACA CTTGCTATCC 
GTGAAGAAGA CCTCAGTGGG CCGCACACAT TCTCTACCTA ATGACAGCTA CATGTTCCTG 
CCGCTGCAGT CCAACTCAAA CCACAGCACC CACACAACCT CACCTCACCT CGCTCAGACA 
GGGTCTACAG CTTCTGTTCA GTCTCAAACA GAAGAAGCCA GCCAGCACTT GACGGTGCCC 
AGCGATCTGT TCAGACCCAT CAGTCCTCAC AGTTTATCAG ACTCAGAGAG CATCCCTCGA 
ATTCCCCCAC CTCGGCGGGC ACACACTCTC TCACGCACAC TCCGCAGACA GGTGGCAGTG 
AGTGCTGACT CTCAGGAGAC GCTTTACACA GAGGGAGGGG AAGCTGAGGG TCCTCTGGGG 
CTCAGGGAAC TGTCGGACCC CCTTCCACCT CAGCAGCAGC AGCACCAGCC TTCCCTCTTT 
CTGGTCCCTG CCACACCAGG CGCCTCCCCA AAACCTTCAC GACCTAGCGT CCACACGCAG 
CACAACCCCT ACGACCAGTA CAATGTGTTC TCCAGGAGTT CCCACTCTCC ACCTGTACCA 
CCACCACCAC CAGACTATAA GAAGCAGGAG GATGTTGACT CGGTGGATCA GGAGGTATCT 
CGAATTATCC GAGCGGGACT CACAGGGAGA AGCGATGATG TCAACCGAGA AGGGACAGGA 
GATCAATGTG CAAAACGTCA GCTTAAGAAA TACCATAGCG TGGACACTCA AGGCCAGCGA 
GCTCTGCTGT TACCGCGGCC CTTGTCATGG CTTGATGACC CACGCCGGCA CTCCATCGAA 
GTATGCTCGT CTGTAGAAAG CAGTCCGCAG CGCAGTTCCA TATCGTCAGG GTTTGTATCA 
CGAGCCGACA GCCTCCAGCA ACAATCTTCG CCCCGTGGAC GCAAGAAGAA GATGAGTCCA 
CCGTGCATTT CTGTAGATCC ACCCGAGGAT CCGGAACTTC CGGGAGGATT CCATCCTGCT 
CTGGGGATTG TGCCACCTCC ATTGCCAAGC CGGGACACTT GCCTGAGGAG GCGCGCTCCC 
TCCAGTGAAT CCAAGGACTC GTTTGATTTG GGAGGAGGAG AGGCGCTGCC TCAAGAGGGC 
GTTCCAAACT CCAAGCTATT GACTCTGCCT AGCTTCTCTT TCGAGAAAAC AAGCTCTGAG 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480344.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336019.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

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CloneID	ODa148360
Clone ID Related Accession (Same CDS sequence)	XM_021480344.1
Accession Version	XM_021480344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT 
CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG 
GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC 
ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG 
CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG 
TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT 
CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG 
GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC 
CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG 
GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT 
CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC 
CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC 
ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT 
GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG 
GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG 
ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC 
CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC 
ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT 
GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG 
AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT 
CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC 
GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA 
GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC 
TCTGAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336019.1
CDS	785..8296
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGKGHVGVRIVKPNPDTMSTVLSSDLAGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480344.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480344.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT 
CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG 
GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC 
ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG 
CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG 
TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT 
CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG 
GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC 
CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG 
GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT 
CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC 
CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC 
ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT 
GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG 
GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG 
ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC 
CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC 
ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT 
GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG 
AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT 
CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC 
GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA 
GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC 
TCTGAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480345.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336020.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

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CloneID	ODa148361
Clone ID Related Accession (Same CDS sequence)	XM_021480345.1
Accession Version	XM_021480345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA ATATAATGCT GACCGGTGTC AGTTGGTCCA GTCCTGATCA CACACAAGAA 
GCCCAGAGTA AACCCTTTTA CTCCGATTAC TGCCCAACAC GACGTCTCAT CTACAACATG 
TGCAAGAGCC AGTATCTGGA CCTGTTCATC ACCATCGTCA TCGCCCTCAA TGTCATCACC 
ATGTCTATGG AGCACTACCA CCAACCAAAG GTTTTGAGCG ATTCCCTAAA GATCTGTAAC 
TACATCTTCA CAGTCATATT TGTGCTGGAG TCCATCTTCA AACTTGTTGC TTTTGGATTT 
CGCCGCTTCT TCAAGGACAG ATGGAACCAG TTGGATCTGG CCATTGTTTT GTTGTCTATC 
ATGGGCATAA CACTGGAGGA AATCGAGGTC AATGCTTCCC TTCCCATCAA CCCTACAATC 
ATTCGCATCA TGAGGGTGTT GCGCATCACT CGAGTTCTAA AGCTACTGAA GATGGCGGTG 
GGAATGCGAG CTCTACTAGA CACTGTGATG CAGGCCCTTC CTCAGGTGGG GAATCTGGGT 
CTTCTCTTCA TGTTGCTTTT CTTCATCTTT GCTGCTCTGG GAGTAGAGCT GTTTGGTGAC 
CTGATATGTG ATGAGCTACA CCCCTGTGAA GGTCTTGGCC GTTATGCCAC GTTCAAGAAT 
TTTGGCATGG CTTTCCTCCT GCTGTTCCGT GTATCCACCG GGGACAACTG GAATGGCATC 
ATGAAGGATA CGCTCAGAGA CTGCTCCCAA GACACCGGTA TATGCTACAA CACGGTGGTG 
TCCCCCATTT ACTTTGTGTC CTTTGTGCTG ACAGCTCAGT TTGTGCTGGT CAATGTGGTG 
ATCGCTGTGC TCATGAAGCA CCTAGAGGAA AGCAACAAAG AGGCCAAGGA GGCTGAAGCA 
GAGGCCGAGC TGGAGCTGGA GCAGGAGATG GAAGCTTTGG GTAGAGATAG AGAAATGCAG 
AGAGGGCACA TGCCACGTTT GGGTTCTGGC GATTTGGGTG GGCCAGGAGG GTCACCTTGG 
ATATCAAGAG ACCATCGAGA CCTGTCAGGA CCACTTTACC CCATAGACAG CCCAACAGCA 
GACATACGCA GAGATTTTGA AGATCATTTA AGGACAGATC CTGATCCACC TCCGAATCTG 
GAGCCACCAT TAGAGCGGAG GCCTATGTTT GACTCAGTTT CCCTGGTGAT CCAGGGCTCG 
CTGGAAGGAG AGCTCAGCCT CATGGACAAC CTGTCTGGCT CCATCTGCCA CTACTACGCT 
CTGCCCCCCC TTCCCAACAA ACACTGCACT GAGAAACAGA TTCCTCTAGC CGAGATGGAG 
GCTCTATCTC TGGCATCGGA GAAATCCTGG TCCTTAGCTC TGACGGATGA CTCTGTCCCA 
GATGATTTTA ACCCCCTCTT ACTAACCAGT CAGGAGTGCA ATACCACAGA TCCTTCAGAT 
CCACCAGACA CCCGGCAGGC GGATGAACCG ACGGATGAAC ACTTGCTATC CGTGAAGAAG 
ACCTCAGTGG GCCGCACACA TTCTCTACCT AATGACAGCT ACATGTTCCT GCCGCTGCAG 
TCCAACTCAA ACCACAGCAC CCACACAACC TCACCTCACC TCGCTCAGAC AGGGTCTACA 
GCTTCTGTTC AGTCTCAAAC AGAAGAAGCC AGCCAGCACT TGACGGTGCC CAGCGATCTG 
TTCAGACCCA TCAGTCCTCA CAGTTTATCA GACTCAGAGA GCATCCCTCG AATTCCCCCA 
CCTCGGCGGG CACACACTCT CTCACGCACA CTCCGCAGAC AGGTGGCAGT GAGTGCTGAC 
TCTCAGGAGA CGCTTTACAC AGAGGGAGGG GAAGCTGAGG GTCCTCTGGG GCTCAGGGAA 
CTGTCGGACC CCCTTCCACC TCAGCAGCAG CAGCACCAGC CTTCCCTCTT TCTGGTCCCT 
GCCACACCAG GCGCCTCCCC AAAACCTTCA CGACCTAGCG TCCACACGCA GCACAACCCC 
TACGACCAGT ACAATGTGTT CTCCAGGAGT TCCCACTCTC CACCTGTACC ACCACCACCA 
CCAGACTATA AGAAGCAGGA GGATGTTGAC TCGGTGGATC AGGAGGTATC TCGAATTATC 
CGAGCGGGAC TCACAGGGAG AAGCGATGAT GTCAACCGAG AAGGGACAGG AGATCAATGT 
GCAAAACGTC AGCTTAAGAA ATACCATAGC GTGGACACTC AAGGCCAGCG AGCTCTGCTG 
TTACCGCGGC CCTTGTCATG GCTTGATGAC CCACGCCGGC ACTCCATCGA AGTATGCTCG 
TCTGTAGAAA GCAGTCCGCA GCGCAGTTCC ATATCGTCAG GGTTTGTATC ACGAGCCGAC 
AGCCTCCAGC AACAATCTTC GCCCCGTGGA CGCAAGAAGA AGATGAGTCC ACCGTGCATT 
TCTGTAGATC CACCCGAGGA TCCGGAACTT CCGGGAGGAT TCCATCCTGC TCTGGGGATT 
GTGCCACCTC CATTGCCAAG CCGGGACACT TGCCTGAGGA GGCGCGCTCC CTCCAGTGAA 
TCCAAGGACT CGTTTGATTT GGGAGGAGGA GAGGCGCTGC CTCAAGAGGG CGTTCCAAAC 
TCCAAGCTAT TGACTCTGCC TAGCTTCTCT TTCGAGAAAA CAAGCTCTGA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336020.1
CDS	784..8220
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480345.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480345.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA ATATAATGCT GACCGGTGTC AGTTGGTCCA GTCCTGATCA CACACAAGAA 
GCCCAGAGTA AACCCTTTTA CTCCGATTAC TGCCCAACAC GACGTCTCAT CTACAACATG 
TGCAAGAGCC AGTATCTGGA CCTGTTCATC ACCATCGTCA TCGCCCTCAA TGTCATCACC 
ATGTCTATGG AGCACTACCA CCAACCAAAG GTTTTGAGCG ATTCCCTAAA GATCTGTAAC 
TACATCTTCA CAGTCATATT TGTGCTGGAG TCCATCTTCA AACTTGTTGC TTTTGGATTT 
CGCCGCTTCT TCAAGGACAG ATGGAACCAG TTGGATCTGG CCATTGTTTT GTTGTCTATC 
ATGGGCATAA CACTGGAGGA AATCGAGGTC AATGCTTCCC TTCCCATCAA CCCTACAATC 
ATTCGCATCA TGAGGGTGTT GCGCATCACT CGAGTTCTAA AGCTACTGAA GATGGCGGTG 
GGAATGCGAG CTCTACTAGA CACTGTGATG CAGGCCCTTC CTCAGGTGGG GAATCTGGGT 
CTTCTCTTCA TGTTGCTTTT CTTCATCTTT GCTGCTCTGG GAGTAGAGCT GTTTGGTGAC 
CTGATATGTG ATGAGCTACA CCCCTGTGAA GGTCTTGGCC GTTATGCCAC GTTCAAGAAT 
TTTGGCATGG CTTTCCTCCT GCTGTTCCGT GTATCCACCG GGGACAACTG GAATGGCATC 
ATGAAGGATA CGCTCAGAGA CTGCTCCCAA GACACCGGTA TATGCTACAA CACGGTGGTG 
TCCCCCATTT ACTTTGTGTC CTTTGTGCTG ACAGCTCAGT TTGTGCTGGT CAATGTGGTG 
ATCGCTGTGC TCATGAAGCA CCTAGAGGAA AGCAACAAAG AGGCCAAGGA GGCTGAAGCA 
GAGGCCGAGC TGGAGCTGGA GCAGGAGATG GAAGCTTTGG GTAGAGATAG AGAAATGCAG 
AGAGGGCACA TGCCACGTTT GGGTTCTGGC GATTTGGGTG GGCCAGGAGG GTCACCTTGG 
ATATCAAGAG ACCATCGAGA CCTGTCAGGA CCACTTTACC CCATAGACAG CCCAACAGCA 
GACATACGCA GAGATTTTGA AGATCATTTA AGGACAGATC CTGATCCACC TCCGAATCTG 
GAGCCACCAT TAGAGCGGAG GCCTATGTTT GACTCAGTTT CCCTGGTGAT CCAGGGCTCG 
CTGGAAGGAG AGCTCAGCCT CATGGACAAC CTGTCTGGCT CCATCTGCCA CTACTACGCT 
CTGCCCCCCC TTCCCAACAA ACACTGCACT GAGAAACAGA TTCCTCTAGC CGAGATGGAG 
GCTCTATCTC TGGCATCGGA GAAATCCTGG TCCTTAGCTC TGACGGATGA CTCTGTCCCA 
GATGATTTTA ACCCCCTCTT ACTAACCAGT CAGGAGTGCA ATACCACAGA TCCTTCAGAT 
CCACCAGACA CCCGGCAGGC GGATGAACCG ACGGATGAAC ACTTGCTATC CGTGAAGAAG 
ACCTCAGTGG GCCGCACACA TTCTCTACCT AATGACAGCT ACATGTTCCT GCCGCTGCAG 
TCCAACTCAA ACCACAGCAC CCACACAACC TCACCTCACC TCGCTCAGAC AGGGTCTACA 
GCTTCTGTTC AGTCTCAAAC AGAAGAAGCC AGCCAGCACT TGACGGTGCC CAGCGATCTG 
TTCAGACCCA TCAGTCCTCA CAGTTTATCA GACTCAGAGA GCATCCCTCG AATTCCCCCA 
CCTCGGCGGG CACACACTCT CTCACGCACA CTCCGCAGAC AGGTGGCAGT GAGTGCTGAC 
TCTCAGGAGA CGCTTTACAC AGAGGGAGGG GAAGCTGAGG GTCCTCTGGG GCTCAGGGAA 
CTGTCGGACC CCCTTCCACC TCAGCAGCAG CAGCACCAGC CTTCCCTCTT TCTGGTCCCT 
GCCACACCAG GCGCCTCCCC AAAACCTTCA CGACCTAGCG TCCACACGCA GCACAACCCC 
TACGACCAGT ACAATGTGTT CTCCAGGAGT TCCCACTCTC CACCTGTACC ACCACCACCA 
CCAGACTATA AGAAGCAGGA GGATGTTGAC TCGGTGGATC AGGAGGTATC TCGAATTATC 
CGAGCGGGAC TCACAGGGAG AAGCGATGAT GTCAACCGAG AAGGGACAGG AGATCAATGT 
GCAAAACGTC AGCTTAAGAA ATACCATAGC GTGGACACTC AAGGCCAGCG AGCTCTGCTG 
TTACCGCGGC CCTTGTCATG GCTTGATGAC CCACGCCGGC ACTCCATCGA AGTATGCTCG 
TCTGTAGAAA GCAGTCCGCA GCGCAGTTCC ATATCGTCAG GGTTTGTATC ACGAGCCGAC 
AGCCTCCAGC AACAATCTTC GCCCCGTGGA CGCAAGAAGA AGATGAGTCC ACCGTGCATT 
TCTGTAGATC CACCCGAGGA TCCGGAACTT CCGGGAGGAT TCCATCCTGC TCTGGGGATT 
GTGCCACCTC CATTGCCAAG CCGGGACACT TGCCTGAGGA GGCGCGCTCC CTCCAGTGAA 
TCCAAGGACT CGTTTGATTT GGGAGGAGGA GAGGCGCTGC CTCAAGAGGG CGTTCCAAAC 
TCCAAGCTAT TGACTCTGCC TAGCTTCTCT TTCGAGAAAA CAAGCTCTGA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_017358537.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214026.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

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CloneID	ODa148362
Clone ID Related Accession (Same CDS sequence)	XM_017358537.2
Accession Version	XM_017358537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA GTAAGGAGAA GGAGCTAGCT GAAGCCCAGA GTAAACCCTT TTACTCCGAT 
TACTGCCCAA CACGACGTCT CATCTACAAC ATGTGCAAGA GCCAGTATCT GGACCTGTTC 
ATCACCATCG TCATCGCCCT CAATGTCATC ACCATGTCTA TGGAGCACTA CCACCAACCA 
AAGGTTTTGA GCGATTCCCT AAAGATCTGT AACTACATCT TCACAGTCAT ATTTGTGCTG 
GAGTCCATCT TCAAACTTGT TGCTTTTGGA TTTCGCCGCT TCTTCAAGGA CAGATGGAAC 
CAGTTGGATC TGGCCATTGT TTTGTTGTCT ATCATGGGCA TAACACTGGA GGAAATCGAG 
GTCAATGCTT CCCTTCCCAT CAACCCTACA ATCATTCGCA TCATGAGGGT GTTGCGCATC 
ACTCGAGTTC TAAAGCTACT GAAGATGGCG GTGGGAATGC GAGCTCTACT AGACACTGTG 
ATGCAGGCCC TTCCTCAGGT GGGGAATCTG GGTCTTCTCT TCATGTTGCT TTTCTTCATC 
TTTGCTGCTC TGGGAGTAGA GCTGTTTGGT GACCTGATAT GTGATGAGCT ACACCCCTGT 
GAAGGTCTTG GCCGTTATGC CACGTTCAAG AATTTTGGCA TGGCTTTCCT CCTGCTGTTC 
CGTGTATCCA CCGGGGACAA CTGGAATGGC ATCATGAAGG ATACGCTCAG AGACTGCTCC 
CAAGACACCG GTATATGCTA CAACACGGTG GTGTCCCCCA TTTACTTTGT GTCCTTTGTG 
CTGACAGCTC AGTTTGTGCT GGTCAATGTG GTGATCGCTG TGCTCATGAA GCACCTAGAG 
GAAAGCAACA AAGAGGCCAA GGAGGCTGAA GCAGAGGCCG AGCTGGAGCT GGAGCAGGAG 
ATGGAAGCTT TGGGTAGAGA TAGAGAAATG CAGAGAGGGC ACATGCCACG TTTGGGTTCT 
GGCGATTTGG GTGGGCCAGG AGGGTCACCT TGGATATCAA GAGACCATCG AGACCTGTCA 
GGACCACTTT ACCCCATAGA CAGCCCAACA GCAGACATAC GCAGAGATTT TGAAGATCAT 
TTAAGGACAG ATCCTGATCC ACCTCCGAAT CTGGAGCCAC CATTAGAGCG GAGGCCTATG 
TTTGACTCAG TTTCCCTGGT GATCCAGGGC TCGCTGGAAG GAGAGCTCAG CCTCATGGAC 
AACCTGTCTG GCTCCATCTG CCACTACTAC GCTCTGCCCC CCCTTCCCAA CAAACACTGC 
ACTGAGAAAC AGATTCCTCT AGCCGAGATG GAGGCTCTAT CTCTGGCATC GGAGAAATCC 
TGGTCCTTAG CTCTGACGGA TGACTCTGTC CCAGATGATT TTAACCCCCT CTTACTAACC 
AGTCAGGAGT GCAATACCAC AGATCCTTCA GATCCACCAG ACACCCGGCA GGCGGATGAA 
CCGACGGATG AACACTTGCT ATCCGTGAAG AAGACCTCAG TGGGCCGCAC ACATTCTCTA 
CCTAATGACA GCTACATGTT CCTGCCGCTG CAGTCCAACT CAAACCACAG CACCCACACA 
ACCTCACCTC ACCTCGCTCA GACAGGGTCT ACAGCTTCTG TTCAGTCTCA AACAGAAGAA 
GCCAGCCAGC ACTTGACGGT GCCCAGCGAT CTGTTCAGAC CCATCAGTCC TCACAGTTTA 
TCAGACTCAG AGAGCATCCC TCGAATTCCC CCACCTCGGC GGGCACACAC TCTCTCACGC 
ACACTCCGCA GACAGGTGGC AGTGAGTGCT GACTCTCAGG AGACGCTTTA CACAGAGGGA 
GGGGAAGCTG AGGGTCCTCT GGGGCTCAGG GAACTGTCGG ACCCCCTTCC ACCTCAGCAG 
CAGCAGCACC AGCCTTCCCT CTTTCTGGTC CCTGCCACAC CAGGCGCCTC CCCAAAACCT 
TCACGACCTA GCGTCCACAC GCAGCACAAC CCCTACGACC AGTACAATGT GTTCTCCAGG 
AGTTCCCACT CTCCACCTGT ACCACCACCA CCACCAGACT ATAAGAAGCA GGAGGATGTT 
GACTCGGTGG ATCAGGAGGT ATCTCGAATT ATCCGAGCGG GACTCACAGG GAGAAGCGAT 
GATGTCAACC GAGAAGGGAC AGGAGATCAA TGTGCAAAAC GTCAGCTTAA GAAATACCAT 
AGCGTGGACA CTCAAGGCCA GCGAGCTCTG CTGTTACCGC GGCCCTTGTC ATGGCTTGAT 
GACCCACGCC GGCACTCCAT CGAAGTATGC TCGTCTGTAG AAAGCAGTCC GCAGCGCAGT 
TCCATATCGT CAGGGTTTGT ATCACGAGCC GACAGCCTCC AGCAACAATC TTCGCCCCGT 
GGACGCAAGA AGAAGATGAG TCCACCGTGC ATTTCTGTAG ATCCACCCGA GGATCCGGAA 
CTTCCGGGAG GATTCCATCC TGCTCTGGGG ATTGTGCCAC CTCCATTGCC AAGCCGGGAC 
ACTTGCCTGA GGAGGCGCGC TCCCTCCAGT GAATCCAAGG ACTCGTTTGA TTTGGGAGGA 
GGAGAGGCGC TGCCTCAAGA GGGCGTTCCA AACTCCAAGC TATTGACTCT GCCTAGCTTC 
TCTTTCGAGA AAACAAGCTC TGAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214026.2
CDS	784..8193
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRSKEKELAEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358537.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358537.2

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA GTAAGGAGAA GGAGCTAGCT GAAGCCCAGA GTAAACCCTT TTACTCCGAT 
TACTGCCCAA CACGACGTCT CATCTACAAC ATGTGCAAGA GCCAGTATCT GGACCTGTTC 
ATCACCATCG TCATCGCCCT CAATGTCATC ACCATGTCTA TGGAGCACTA CCACCAACCA 
AAGGTTTTGA GCGATTCCCT AAAGATCTGT AACTACATCT TCACAGTCAT ATTTGTGCTG 
GAGTCCATCT TCAAACTTGT TGCTTTTGGA TTTCGCCGCT TCTTCAAGGA CAGATGGAAC 
CAGTTGGATC TGGCCATTGT TTTGTTGTCT ATCATGGGCA TAACACTGGA GGAAATCGAG 
GTCAATGCTT CCCTTCCCAT CAACCCTACA ATCATTCGCA TCATGAGGGT GTTGCGCATC 
ACTCGAGTTC TAAAGCTACT GAAGATGGCG GTGGGAATGC GAGCTCTACT AGACACTGTG 
ATGCAGGCCC TTCCTCAGGT GGGGAATCTG GGTCTTCTCT TCATGTTGCT TTTCTTCATC 
TTTGCTGCTC TGGGAGTAGA GCTGTTTGGT GACCTGATAT GTGATGAGCT ACACCCCTGT 
GAAGGTCTTG GCCGTTATGC CACGTTCAAG AATTTTGGCA TGGCTTTCCT CCTGCTGTTC 
CGTGTATCCA CCGGGGACAA CTGGAATGGC ATCATGAAGG ATACGCTCAG AGACTGCTCC 
CAAGACACCG GTATATGCTA CAACACGGTG GTGTCCCCCA TTTACTTTGT GTCCTTTGTG 
CTGACAGCTC AGTTTGTGCT GGTCAATGTG GTGATCGCTG TGCTCATGAA GCACCTAGAG 
GAAAGCAACA AAGAGGCCAA GGAGGCTGAA GCAGAGGCCG AGCTGGAGCT GGAGCAGGAG 
ATGGAAGCTT TGGGTAGAGA TAGAGAAATG CAGAGAGGGC ACATGCCACG TTTGGGTTCT 
GGCGATTTGG GTGGGCCAGG AGGGTCACCT TGGATATCAA GAGACCATCG AGACCTGTCA 
GGACCACTTT ACCCCATAGA CAGCCCAACA GCAGACATAC GCAGAGATTT TGAAGATCAT 
TTAAGGACAG ATCCTGATCC ACCTCCGAAT CTGGAGCCAC CATTAGAGCG GAGGCCTATG 
TTTGACTCAG TTTCCCTGGT GATCCAGGGC TCGCTGGAAG GAGAGCTCAG CCTCATGGAC 
AACCTGTCTG GCTCCATCTG CCACTACTAC GCTCTGCCCC CCCTTCCCAA CAAACACTGC 
ACTGAGAAAC AGATTCCTCT AGCCGAGATG GAGGCTCTAT CTCTGGCATC GGAGAAATCC 
TGGTCCTTAG CTCTGACGGA TGACTCTGTC CCAGATGATT TTAACCCCCT CTTACTAACC 
AGTCAGGAGT GCAATACCAC AGATCCTTCA GATCCACCAG ACACCCGGCA GGCGGATGAA 
CCGACGGATG AACACTTGCT ATCCGTGAAG AAGACCTCAG TGGGCCGCAC ACATTCTCTA 
CCTAATGACA GCTACATGTT CCTGCCGCTG CAGTCCAACT CAAACCACAG CACCCACACA 
ACCTCACCTC ACCTCGCTCA GACAGGGTCT ACAGCTTCTG TTCAGTCTCA AACAGAAGAA 
GCCAGCCAGC ACTTGACGGT GCCCAGCGAT CTGTTCAGAC CCATCAGTCC TCACAGTTTA 
TCAGACTCAG AGAGCATCCC TCGAATTCCC CCACCTCGGC GGGCACACAC TCTCTCACGC 
ACACTCCGCA GACAGGTGGC AGTGAGTGCT GACTCTCAGG AGACGCTTTA CACAGAGGGA 
GGGGAAGCTG AGGGTCCTCT GGGGCTCAGG GAACTGTCGG ACCCCCTTCC ACCTCAGCAG 
CAGCAGCACC AGCCTTCCCT CTTTCTGGTC CCTGCCACAC CAGGCGCCTC CCCAAAACCT 
TCACGACCTA GCGTCCACAC GCAGCACAAC CCCTACGACC AGTACAATGT GTTCTCCAGG 
AGTTCCCACT CTCCACCTGT ACCACCACCA CCACCAGACT ATAAGAAGCA GGAGGATGTT 
GACTCGGTGG ATCAGGAGGT ATCTCGAATT ATCCGAGCGG GACTCACAGG GAGAAGCGAT 
GATGTCAACC GAGAAGGGAC AGGAGATCAA TGTGCAAAAC GTCAGCTTAA GAAATACCAT 
AGCGTGGACA CTCAAGGCCA GCGAGCTCTG CTGTTACCGC GGCCCTTGTC ATGGCTTGAT 
GACCCACGCC GGCACTCCAT CGAAGTATGC TCGTCTGTAG AAAGCAGTCC GCAGCGCAGT 
TCCATATCGT CAGGGTTTGT ATCACGAGCC GACAGCCTCC AGCAACAATC TTCGCCCCGT 
GGACGCAAGA AGAAGATGAG TCCACCGTGC ATTTCTGTAG ATCCACCCGA GGATCCGGAA 
CTTCCGGGAG GATTCCATCC TGCTCTGGGG ATTGTGCCAC CTCCATTGCC AAGCCGGGAC 
ACTTGCCTGA GGAGGCGCGC TCCCTCCAGT GAATCCAAGG ACTCGTTTGA TTTGGGAGGA 
GGAGAGGCGC TGCCTCAAGA GGGCGTTCCA AACTCCAAGC TATTGACTCT GCCTAGCTTC 
TCTTTCGAGA AAACAAGCTC TGAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480346.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336021.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.

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CloneID	ODa148363
Clone ID Related Accession (Same CDS sequence)	XM_021480346.1
Accession Version	XM_021480346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA AAGCCCAGAG TAAACCCTTT TACTCCGATT ACTGCCCAAC ACGACGTCTC 
ATCTACAACA TGTGCAAGAG CCAGTATCTG GACCTGTTCA TCACCATCGT CATCGCCCTC 
AATGTCATCA CCATGTCTAT GGAGCACTAC CACCAACCAA AGGTTTTGAG CGATTCCCTA 
AAGATCTGTA ACTACATCTT CACAGTCATA TTTGTGCTGG AGTCCATCTT CAAACTTGTT 
GCTTTTGGAT TTCGCCGCTT CTTCAAGGAC AGATGGAACC AGTTGGATCT GGCCATTGTT 
TTGTTGTCTA TCATGGGCAT AACACTGGAG GAAATCGAGG TCAATGCTTC CCTTCCCATC 
AACCCTACAA TCATTCGCAT CATGAGGGTG TTGCGCATCA CTCGAGTTCT AAAGCTACTG 
AAGATGGCGG TGGGAATGCG AGCTCTACTA GACACTGTGA TGCAGGCCCT TCCTCAGGTG 
GGGAATCTGG GTCTTCTCTT CATGTTGCTT TTCTTCATCT TTGCTGCTCT GGGAGTAGAG 
CTGTTTGGTG ACCTGATATG TGATGAGCTA CACCCCTGTG AAGGTCTTGG CCGTTATGCC 
ACGTTCAAGA ATTTTGGCAT GGCTTTCCTC CTGCTGTTCC GTGTATCCAC CGGGGACAAC 
TGGAATGGCA TCATGAAGGA TACGCTCAGA GACTGCTCCC AAGACACCGG TATATGCTAC 
AACACGGTGG TGTCCCCCAT TTACTTTGTG TCCTTTGTGC TGACAGCTCA GTTTGTGCTG 
GTCAATGTGG TGATCGCTGT GCTCATGAAG CACCTAGAGG AAAGCAACAA AGAGGCCAAG 
GAGGCTGAAG CAGAGGCCGA GCTGGAGCTG GAGCAGGAGA TGGAAGCTTT GGGTAGAGAT 
AGAGAAATGC AGAGAGGGCA CATGCCACGT TTGGGTTCTG GCGATTTGGG TGGGCCAGGA 
GGGTCACCTT GGATATCAAG AGACCATCGA GACCTGTCAG GACCACTTTA CCCCATAGAC 
AGCCCAACAG CAGACATACG CAGAGATTTT GAAGATCATT TAAGGACAGA TCCTGATCCA 
CCTCCGAATC TGGAGCCACC ATTAGAGCGG AGGCCTATGT TTGACTCAGT TTCCCTGGTG 
ATCCAGGGCT CGCTGGAAGG AGAGCTCAGC CTCATGGACA ACCTGTCTGG CTCCATCTGC 
CACTACTACG CTCTGCCCCC CCTTCCCAAC AAACACTGCA CTGAGAAACA GATTCCTCTA 
GCCGAGATGG AGGCTCTATC TCTGGCATCG GAGAAATCCT GGTCCTTAGC TCTGACGGAT 
GACTCTGTCC CAGATGATTT TAACCCCCTC TTACTAACCA GTCAGGAGTG CAATACCACA 
GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA 
TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC 
CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG 
ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG 
CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT 
CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA 
GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG 
GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC 
TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG 
CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA 
CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA 
TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA 
GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG 
CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC 
GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA 
TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT 
CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT 
GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT 
CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG 
GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT 
GAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336021.1
CDS	783..8171
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRKAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480346.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480346.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGGGTGA TGCCTCAAAG 
TCGGACTCTG AAGCCGATAT TTATGCACGG AGCTTGGAGG ATGTTTCGGG ACACAAGAAA 
GATATGTCTG CATCATCCGT TGTGCCCATC AATGGCCATG TGGATCTGAA GAGCAGCCTG 
ACACCGCCTC TGATCACCCA CACAGCTGCC ACACCAATGC CCATCCCCAA GATATCAGGA 
GGAGGAGATT CAGCACTGAG CCAAGAGTCC CGCAGAGGCA GCAGTGTCTC CATGGAGCCT 
AATGGCCAAG ACAAATCTCC ATCCAGTGCA CGGAGCTCCC CTAATGCCCC CTTGAGCTCC 
GCCAGTAGCT GGAACAGCAG ACGCTCCAGC TGGAACAGTC TAGGCCGAGC CCCCAGCCTC 
AAGAGGCAGA AACACCAGTC TGGTGAAAGG CGCTCCTTGT TGTCTGGTGA TGGCCAGTCC 
AGTTCTGATG AGGGGGATGC AGGAGAGAGC ATGGGGGGCA TGTCTGAGGG TGATGATGCC 
TCCCTCGCCA GGACGGACTC GCTGGGCCAG AGGCCTCGAC ATAGGCGCAT GGAGTCCCTG 
GAGACGCGCA GCTCATTTGA TCTCCATCCA GACACCCTGC AGGTGCCGTA CCCCCACCGC 
TCCGGCAGCA TACACAGCGC ACGGCCACCC AACTTCCTCA GCAACGGCAA GACTTCTCCA 
TCTGCAGCCA CCACACAGCT CTCTTTGGAT GAACACCACA GTGAAGACGA CAACGCAGAC 
GAGGAAGGAA ACCTGAGTCG CAGGGCTCGT CTGTACAGAT GGCTGGAGCA CAAGCAGCCC 
GAATGGTGTC GGCAGAGGGT CACCTGGTCC CTTTACCTCT TTCCTCCAGA GTCACGGTTT 
CGCGTGACAT GCAATAAAAT CATCACCCAC AAGATGTTTG ACCATGTGGT TCTTGTCATC 
ATCTTTCTCA ACTGCATCAC CATCGCCATG GAGAGGCCCA GAATCGACCC CTCTAGCGCT 
GAACGCATCT TCTTGACGTT GTCCAACTAC ATATTTACTG CCATCTTTGT GACCGAAATG 
ACAATAAAGG TGGTTGCTTT GGGCTTTTGT TTTGGAGAGA AGACCTACCT GAAGAGCAGC 
TGGAACATTC TGGATGGCAT GCTGGTGATG ATCTCTGTGA TTGACATCCT CGTGTCCCTG 
ATCTCCAACA GTGGCACCAA GATCCTGGGC ATGTTACGTG TGCTGCGGTT ACTGAGGACA 
CTCAGGCCAC TCAGAGTGAT CAGCAGAGCT CCTGGACTGA AACTGGTTGT TGAGACATTG 
ATGTCGTCTC TTAAGCCTAT CGGAAACATT GTGGTCATCT GCTGTGCCTT CTTCATTATT 
TTTGGGATCT TGGGAGTTCA GCTTTTCAAG GGAAAGTTCT TTGTGTGTCA TGGGGATGAT 
ACGAGAAACA TCACCAACAA GTCAGATTGC CTCCTGGCAA ACTACAAATG GGTCAGACAC 
AAGTACAACT TTGATAACCT TGGACAGGCC CTCATGTCCC TTTTTGTTCT GGCTTCCAAA 
GACGGTTGGG TGGATATCAT GTACGATGGA CTGGATGCTG TCGGTGTGGA CCAGCAGCCC 
GTCATGAATT ACAACCCATG GATGCTGCTC TATTTTATCT CCTTCTTGCT GATCGTGGCT 
TTCTTCGTGC TCAACATGTT TGTCGGCGTG GTCGTCGAGA ACTTTCACAA GTGCAGGAGA 
AACCAGGAGG AGGAAGAAGC CAAACGGCGA GAAGCGAAGC GCCAGAAACG ACGTGATAAA 
AAACGAAGGA AAGCCCAGAG TAAACCCTTT TACTCCGATT ACTGCCCAAC ACGACGTCTC 
ATCTACAACA TGTGCAAGAG CCAGTATCTG GACCTGTTCA TCACCATCGT CATCGCCCTC 
AATGTCATCA CCATGTCTAT GGAGCACTAC CACCAACCAA AGGTTTTGAG CGATTCCCTA 
AAGATCTGTA ACTACATCTT CACAGTCATA TTTGTGCTGG AGTCCATCTT CAAACTTGTT 
GCTTTTGGAT TTCGCCGCTT CTTCAAGGAC AGATGGAACC AGTTGGATCT GGCCATTGTT 
TTGTTGTCTA TCATGGGCAT AACACTGGAG GAAATCGAGG TCAATGCTTC CCTTCCCATC 
AACCCTACAA TCATTCGCAT CATGAGGGTG TTGCGCATCA CTCGAGTTCT AAAGCTACTG 
AAGATGGCGG TGGGAATGCG AGCTCTACTA GACACTGTGA TGCAGGCCCT TCCTCAGGTG 
GGGAATCTGG GTCTTCTCTT CATGTTGCTT TTCTTCATCT TTGCTGCTCT GGGAGTAGAG 
CTGTTTGGTG ACCTGATATG TGATGAGCTA CACCCCTGTG AAGGTCTTGG CCGTTATGCC 
ACGTTCAAGA ATTTTGGCAT GGCTTTCCTC CTGCTGTTCC GTGTATCCAC CGGGGACAAC 
TGGAATGGCA TCATGAAGGA TACGCTCAGA GACTGCTCCC AAGACACCGG TATATGCTAC 
AACACGGTGG TGTCCCCCAT TTACTTTGTG TCCTTTGTGC TGACAGCTCA GTTTGTGCTG 
GTCAATGTGG TGATCGCTGT GCTCATGAAG CACCTAGAGG AAAGCAACAA AGAGGCCAAG 
GAGGCTGAAG CAGAGGCCGA GCTGGAGCTG GAGCAGGAGA TGGAAGCTTT GGGTAGAGAT 
AGAGAAATGC AGAGAGGGCA CATGCCACGT TTGGGTTCTG GCGATTTGGG TGGGCCAGGA 
GGGTCACCTT GGATATCAAG AGACCATCGA GACCTGTCAG GACCACTTTA CCCCATAGAC 
AGCCCAACAG CAGACATACG CAGAGATTTT GAAGATCATT TAAGGACAGA TCCTGATCCA 
CCTCCGAATC TGGAGCCACC ATTAGAGCGG AGGCCTATGT TTGACTCAGT TTCCCTGGTG 
ATCCAGGGCT CGCTGGAAGG AGAGCTCAGC CTCATGGACA ACCTGTCTGG CTCCATCTGC 
CACTACTACG CTCTGCCCCC CCTTCCCAAC AAACACTGCA CTGAGAAACA GATTCCTCTA 
GCCGAGATGG AGGCTCTATC TCTGGCATCG GAGAAATCCT GGTCCTTAGC TCTGACGGAT 
GACTCTGTCC CAGATGATTT TAACCCCCTC TTACTAACCA GTCAGGAGTG CAATACCACA 
GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA 
TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC 
CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG 
ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG 
CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT 
CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA 
GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG 
GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC 
TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG 
CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA 
CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA 
TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA 
GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG 
CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC 
GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA 
TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT 
CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT 
GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT 
CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG 
GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT 
GAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480347.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336022.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.

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CloneID	ODa148364
Clone ID Related Accession (Same CDS sequence)	XM_021480347.1
Accession Version	XM_021480347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGACCACA 
GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA 
TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC 
CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG 
ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG 
CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT 
CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA 
GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG 
GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC 
TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG 
CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA 
CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA 
TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA 
GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG 
CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC 
GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA 
TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT 
CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT 
GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT 
CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG 
GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT 
GAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336022.1
CDS	784..8172
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGKGHVGVRIVKPNPDTMSTVLSSDLAGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480347.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480347.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGACCACA 
GATCCTTCAG ATCCACCAGA CACCCGGCAG GCGGATGAAC CGACGGATGA ACACTTGCTA 
TCCGTGAAGA AGACCTCAGT GGGCCGCACA CATTCTCTAC CTAATGACAG CTACATGTTC 
CTGCCGCTGC AGTCCAACTC AAACCACAGC ACCCACACAA CCTCACCTCA CCTCGCTCAG 
ACAGGGTCTA CAGCTTCTGT TCAGTCTCAA ACAGAAGAAG CCAGCCAGCA CTTGACGGTG 
CCCAGCGATC TGTTCAGACC CATCAGTCCT CACAGTTTAT CAGACTCAGA GAGCATCCCT 
CGAATTCCCC CACCTCGGCG GGCACACACT CTCTCACGCA CACTCCGCAG ACAGGTGGCA 
GTGAGTGCTG ACTCTCAGGA GACGCTTTAC ACAGAGGGAG GGGAAGCTGA GGGTCCTCTG 
GGGCTCAGGG AACTGTCGGA CCCCCTTCCA CCTCAGCAGC AGCAGCACCA GCCTTCCCTC 
TTTCTGGTCC CTGCCACACC AGGCGCCTCC CCAAAACCTT CACGACCTAG CGTCCACACG 
CAGCACAACC CCTACGACCA GTACAATGTG TTCTCCAGGA GTTCCCACTC TCCACCTGTA 
CCACCACCAC CACCAGACTA TAAGAAGCAG GAGGATGTTG ACTCGGTGGA TCAGGAGGTA 
TCTCGAATTA TCCGAGCGGG ACTCACAGGG AGAAGCGATG ATGTCAACCG AGAAGGGACA 
GGAGATCAAT GTGCAAAACG TCAGCTTAAG AAATACCATA GCGTGGACAC TCAAGGCCAG 
CGAGCTCTGC TGTTACCGCG GCCCTTGTCA TGGCTTGATG ACCCACGCCG GCACTCCATC 
GAAGTATGCT CGTCTGTAGA AAGCAGTCCG CAGCGCAGTT CCATATCGTC AGGGTTTGTA 
TCACGAGCCG ACAGCCTCCA GCAACAATCT TCGCCCCGTG GACGCAAGAA GAAGATGAGT 
CCACCGTGCA TTTCTGTAGA TCCACCCGAG GATCCGGAAC TTCCGGGAGG ATTCCATCCT 
GCTCTGGGGA TTGTGCCACC TCCATTGCCA AGCCGGGACA CTTGCCTGAG GAGGCGCGCT 
CCCTCCAGTG AATCCAAGGA CTCGTTTGAT TTGGGAGGAG GAGAGGCGCT GCCTCAAGAG 
GGCGTTCCAA ACTCCAAGCT ATTGACTCTG CCTAGCTTCT CTTTCGAGAA AACAAGCTCT 
GAGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_017358535.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214024.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.

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CloneID	ODa148365
Clone ID Related Accession (Same CDS sequence)	XM_017358535.2
Accession Version	XM_017358535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAATATAA TGCTGACCGG TGTCAGTTGG TCCAGTCCTG ATCACACACA AGAAGCCCAG 
AGTAAACCCT TTTACTCCGA TTACTGCCCA ACACGACGTC TCATCTACAA CATGTGCAAG 
AGCCAGTATC TGGACCTGTT CATCACCATC GTCATCGCCC TCAATGTCAT CACCATGTCT 
ATGGAGCACT ACCACCAACC AAAGGTTTTG AGCGATTCCC TAAAGATCTG TAACTACATC 
TTCACAGTCA TATTTGTGCT GGAGTCCATC TTCAAACTTG TTGCTTTTGG ATTTCGCCGC 
TTCTTCAAGG ACAGATGGAA CCAGTTGGAT CTGGCCATTG TTTTGTTGTC TATCATGGGC 
ATAACACTGG AGGAAATCGA GGTCAATGCT TCCCTTCCCA TCAACCCTAC AATCATTCGC 
ATCATGAGGG TGTTGCGCAT CACTCGAGTT CTAAAGCTAC TGAAGATGGC GGTGGGAATG 
CGAGCTCTAC TAGACACTGT GATGCAGGCC CTTCCTCAGG TGGGGAATCT GGGTCTTCTC 
TTCATGTTGC TTTTCTTCAT CTTTGCTGCT CTGGGAGTAG AGCTGTTTGG TGACCTGATA 
TGTGATGAGC TACACCCCTG TGAAGGTCTT GGCCGTTATG CCACGTTCAA GAATTTTGGC 
ATGGCTTTCC TCCTGCTGTT CCGTGTATCC ACCGGGGACA ACTGGAATGG CATCATGAAG 
GATACGCTCA GAGACTGCTC CCAAGACACC GGTATATGCT ACAACACGGT GGTGTCCCCC 
ATTTACTTTG TGTCCTTTGT GCTGACAGCT CAGTTTGTGC TGGTCAATGT GGTGATCGCT 
GTGCTCATGA AGCACCTAGA GGAAAGCAAC AAAGAGGCCA AGGAGGCTGA AGCAGAGGCC 
GAGCTGGAGC TGGAGCAGGA GATGGAAGCT TTGGGTAGAG ATAGAGAAAT GCAGAGAGGG 
CACATGCCAC GTTTGGGTTC TGGCGATTTG GGTGGGCCAG GAGGGTCACC TTGGATATCA 
AGAGACCATC GAGACCTGTC AGGACCACTT TACCCCATAG ACAGCCCAAC AGCAGACATA 
CGCAGAGATT TTGAAGATCA TTTAAGGACA GATCCTGATC CACCTCCGAA TCTGGAGCCA 
CCATTAGAGC GGAGGCCTAT GTTTGACTCA GTTTCCCTGG TGATCCAGGG CTCGCTGGAA 
GGAGAGCTCA GCCTCATGGA CAACCTGTCT GGCTCCATCT GCCACTACTA CGCTCTGCCC 
CCCCTTCCCA ACAAACACTG CACTGAGAAA CAGATTCCTC TAGCCGAGAT GGAGGCTCTA 
TCTCTGGCAT CGGAGAAATC CTGGTCCTTA GCTCTGACGG ATGACTCTGT CCCAGATGAT 
TTTAACCCCC TCTTACTAAC CAGTCAGGAG TGCAATACCA CAGATCCTTC AGATCCACCA 
GACACCCGGC AGGCGGATGA ACCGACGGAT GAACACTTGC TATCCGTGAA GAAGACCTCA 
GTGGGCCGCA CACATTCTCT ACCTAATGAC AGCTACATGT TCCTGCCGCT GCAGTCCAAC 
TCAAACCACA GCACCCACAC AACCTCACCT CACCTCGCTC AGACAGGGTC TACAGCTTCT 
GTTCAGTCTC AAACAGAAGA AGCCAGCCAG CACTTGACGG TGCCCAGCGA TCTGTTCAGA 
CCCATCAGTC CTCACAGTTT ATCAGACTCA GAGAGCATCC CTCGAATTCC CCCACCTCGG 
CGGGCACACA CTCTCTCACG CACACTCCGC AGACAGGTGG CAGTGAGTGC TGACTCTCAG 
GAGACGCTTT ACACAGAGGG AGGGGAAGCT GAGGGTCCTC TGGGGCTCAG GGAACTGTCG 
GACCCCCTTC CACCTCAGCA GCAGCAGCAC CAGCCTTCCC TCTTTCTGGT CCCTGCCACA 
CCAGGCGCCT CCCCAAAACC TTCACGACCT AGCGTCCACA CGCAGCACAA CCCCTACGAC 
CAGTACAATG TGTTCTCCAG GAGTTCCCAC TCTCCACCTG TACCACCACC ACCACCAGAC 
TATAAGAAGC AGGAGGATGT TGACTCGGTG GATCAGGAGG TATCTCGAAT TATCCGAGCG 
GGACTCACAG GGAGAAGCGA TGATGTCAAC CGAGAAGGGA CAGGAGATCA ATGTGCAAAA 
CGTCAGCTTA AGAAATACCA TAGCGTGGAC ACTCAAGGCC AGCGAGCTCT GCTGTTACCG 
CGGCCCTTGT CATGGCTTGA TGACCCACGC CGGCACTCCA TCGAAGTATG CTCGTCTGTA 
GAAAGCAGTC CGCAGCGCAG TTCCATATCG TCAGGGTTTG TATCACGAGC CGACAGCCTC 
CAGCAACAAT CTTCGCCCCG TGGACGCAAG AAGAAGATGA GTCCACCGTG CATTTCTGTA 
GATCCACCCG AGGATCCGGA ACTTCCGGGA GGATTCCATC CTGCTCTGGG GATTGTGCCA 
CCTCCATTGC CAAGCCGGGA CACTTGCCTG AGGAGGCGCG CTCCCTCCAG TGAATCCAAG 
GACTCGTTTG ATTTGGGAGG AGGAGAGGCG CTGCCTCAAG AGGGCGTTCC AAACTCCAAG 
CTATTGACTC TGCCTAGCTT CTCTTTCGAG AAAACAAGCT CTGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214024.2
CDS	783..8153
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358535.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358535.2

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAATATAA TGCTGACCGG TGTCAGTTGG TCCAGTCCTG ATCACACACA AGAAGCCCAG 
AGTAAACCCT TTTACTCCGA TTACTGCCCA ACACGACGTC TCATCTACAA CATGTGCAAG 
AGCCAGTATC TGGACCTGTT CATCACCATC GTCATCGCCC TCAATGTCAT CACCATGTCT 
ATGGAGCACT ACCACCAACC AAAGGTTTTG AGCGATTCCC TAAAGATCTG TAACTACATC 
TTCACAGTCA TATTTGTGCT GGAGTCCATC TTCAAACTTG TTGCTTTTGG ATTTCGCCGC 
TTCTTCAAGG ACAGATGGAA CCAGTTGGAT CTGGCCATTG TTTTGTTGTC TATCATGGGC 
ATAACACTGG AGGAAATCGA GGTCAATGCT TCCCTTCCCA TCAACCCTAC AATCATTCGC 
ATCATGAGGG TGTTGCGCAT CACTCGAGTT CTAAAGCTAC TGAAGATGGC GGTGGGAATG 
CGAGCTCTAC TAGACACTGT GATGCAGGCC CTTCCTCAGG TGGGGAATCT GGGTCTTCTC 
TTCATGTTGC TTTTCTTCAT CTTTGCTGCT CTGGGAGTAG AGCTGTTTGG TGACCTGATA 
TGTGATGAGC TACACCCCTG TGAAGGTCTT GGCCGTTATG CCACGTTCAA GAATTTTGGC 
ATGGCTTTCC TCCTGCTGTT CCGTGTATCC ACCGGGGACA ACTGGAATGG CATCATGAAG 
GATACGCTCA GAGACTGCTC CCAAGACACC GGTATATGCT ACAACACGGT GGTGTCCCCC 
ATTTACTTTG TGTCCTTTGT GCTGACAGCT CAGTTTGTGC TGGTCAATGT GGTGATCGCT 
GTGCTCATGA AGCACCTAGA GGAAAGCAAC AAAGAGGCCA AGGAGGCTGA AGCAGAGGCC 
GAGCTGGAGC TGGAGCAGGA GATGGAAGCT TTGGGTAGAG ATAGAGAAAT GCAGAGAGGG 
CACATGCCAC GTTTGGGTTC TGGCGATTTG GGTGGGCCAG GAGGGTCACC TTGGATATCA 
AGAGACCATC GAGACCTGTC AGGACCACTT TACCCCATAG ACAGCCCAAC AGCAGACATA 
CGCAGAGATT TTGAAGATCA TTTAAGGACA GATCCTGATC CACCTCCGAA TCTGGAGCCA 
CCATTAGAGC GGAGGCCTAT GTTTGACTCA GTTTCCCTGG TGATCCAGGG CTCGCTGGAA 
GGAGAGCTCA GCCTCATGGA CAACCTGTCT GGCTCCATCT GCCACTACTA CGCTCTGCCC 
CCCCTTCCCA ACAAACACTG CACTGAGAAA CAGATTCCTC TAGCCGAGAT GGAGGCTCTA 
TCTCTGGCAT CGGAGAAATC CTGGTCCTTA GCTCTGACGG ATGACTCTGT CCCAGATGAT 
TTTAACCCCC TCTTACTAAC CAGTCAGGAG TGCAATACCA CAGATCCTTC AGATCCACCA 
GACACCCGGC AGGCGGATGA ACCGACGGAT GAACACTTGC TATCCGTGAA GAAGACCTCA 
GTGGGCCGCA CACATTCTCT ACCTAATGAC AGCTACATGT TCCTGCCGCT GCAGTCCAAC 
TCAAACCACA GCACCCACAC AACCTCACCT CACCTCGCTC AGACAGGGTC TACAGCTTCT 
GTTCAGTCTC AAACAGAAGA AGCCAGCCAG CACTTGACGG TGCCCAGCGA TCTGTTCAGA 
CCCATCAGTC CTCACAGTTT ATCAGACTCA GAGAGCATCC CTCGAATTCC CCCACCTCGG 
CGGGCACACA CTCTCTCACG CACACTCCGC AGACAGGTGG CAGTGAGTGC TGACTCTCAG 
GAGACGCTTT ACACAGAGGG AGGGGAAGCT GAGGGTCCTC TGGGGCTCAG GGAACTGTCG 
GACCCCCTTC CACCTCAGCA GCAGCAGCAC CAGCCTTCCC TCTTTCTGGT CCCTGCCACA 
CCAGGCGCCT CCCCAAAACC TTCACGACCT AGCGTCCACA CGCAGCACAA CCCCTACGAC 
CAGTACAATG TGTTCTCCAG GAGTTCCCAC TCTCCACCTG TACCACCACC ACCACCAGAC 
TATAAGAAGC AGGAGGATGT TGACTCGGTG GATCAGGAGG TATCTCGAAT TATCCGAGCG 
GGACTCACAG GGAGAAGCGA TGATGTCAAC CGAGAAGGGA CAGGAGATCA ATGTGCAAAA 
CGTCAGCTTA AGAAATACCA TAGCGTGGAC ACTCAAGGCC AGCGAGCTCT GCTGTTACCG 
CGGCCCTTGT CATGGCTTGA TGACCCACGC CGGCACTCCA TCGAAGTATG CTCGTCTGTA 
GAAAGCAGTC CGCAGCGCAG TTCCATATCG TCAGGGTTTG TATCACGAGC CGACAGCCTC 
CAGCAACAAT CTTCGCCCCG TGGACGCAAG AAGAAGATGA GTCCACCGTG CATTTCTGTA 
GATCCACCCG AGGATCCGGA ACTTCCGGGA GGATTCCATC CTGCTCTGGG GATTGTGCCA 
CCTCCATTGC CAAGCCGGGA CACTTGCCTG AGGAGGCGCG CTCCCTCCAG TGAATCCAAG 
GACTCGTTTG ATTTGGGAGG AGGAGAGGCG CTGCCTCAAG AGGGCGTTCC AAACTCCAAG 
CTATTGACTC TGCCTAGCTT CTCTTTCGAG AAAACAAGCT CTGAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_017358536.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214025.2 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA.

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CloneID	ODa148366
Clone ID Related Accession (Same CDS sequence)	XM_017358536.2
Accession Version	XM_017358536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAGTAAGG AGAAGGAGCT AGCTGAAGCC CAGAGTAAAC CCTTTTACTC CGATTACTGC 
CCAACACGAC GTCTCATCTA CAACATGTGC AAGAGCCAGT ATCTGGACCT GTTCATCACC 
ATCGTCATCG CCCTCAATGT CATCACCATG TCTATGGAGC ACTACCACCA ACCAAAGGTT 
TTGAGCGATT CCCTAAAGAT CTGTAACTAC ATCTTCACAG TCATATTTGT GCTGGAGTCC 
ATCTTCAAAC TTGTTGCTTT TGGATTTCGC CGCTTCTTCA AGGACAGATG GAACCAGTTG 
GATCTGGCCA TTGTTTTGTT GTCTATCATG GGCATAACAC TGGAGGAAAT CGAGGTCAAT 
GCTTCCCTTC CCATCAACCC TACAATCATT CGCATCATGA GGGTGTTGCG CATCACTCGA 
GTTCTAAAGC TACTGAAGAT GGCGGTGGGA ATGCGAGCTC TACTAGACAC TGTGATGCAG 
GCCCTTCCTC AGGTGGGGAA TCTGGGTCTT CTCTTCATGT TGCTTTTCTT CATCTTTGCT 
GCTCTGGGAG TAGAGCTGTT TGGTGACCTG ATATGTGATG AGCTACACCC CTGTGAAGGT 
CTTGGCCGTT ATGCCACGTT CAAGAATTTT GGCATGGCTT TCCTCCTGCT GTTCCGTGTA 
TCCACCGGGG ACAACTGGAA TGGCATCATG AAGGATACGC TCAGAGACTG CTCCCAAGAC 
ACCGGTATAT GCTACAACAC GGTGGTGTCC CCCATTTACT TTGTGTCCTT TGTGCTGACA 
GCTCAGTTTG TGCTGGTCAA TGTGGTGATC GCTGTGCTCA TGAAGCACCT AGAGGAAAGC 
AACAAAGAGG CCAAGGAGGC TGAAGCAGAG GCCGAGCTGG AGCTGGAGCA GGAGATGGAA 
GCTTTGGGTA GAGATAGAGA AATGCAGAGA GGGCACATGC CACGTTTGGG TTCTGGCGAT 
TTGGGTGGGC CAGGAGGGTC ACCTTGGATA TCAAGAGACC ATCGAGACCT GTCAGGACCA 
CTTTACCCCA TAGACAGCCC AACAGCAGAC ATACGCAGAG ATTTTGAAGA TCATTTAAGG 
ACAGATCCTG ATCCACCTCC GAATCTGGAG CCACCATTAG AGCGGAGGCC TATGTTTGAC 
TCAGTTTCCC TGGTGATCCA GGGCTCGCTG GAAGGAGAGC TCAGCCTCAT GGACAACCTG 
TCTGGCTCCA TCTGCCACTA CTACGCTCTG CCCCCCCTTC CCAACAAACA CTGCACTGAG 
AAACAGATTC CTCTAGCCGA GATGGAGGCT CTATCTCTGG CATCGGAGAA ATCCTGGTCC 
TTAGCTCTGA CGGATGACTC TGTCCCAGAT GATTTTAACC CCCTCTTACT AACCAGTCAG 
GAGTGCAATA CCACAGATCC TTCAGATCCA CCAGACACCC GGCAGGCGGA TGAACCGACG 
GATGAACACT TGCTATCCGT GAAGAAGACC TCAGTGGGCC GCACACATTC TCTACCTAAT 
GACAGCTACA TGTTCCTGCC GCTGCAGTCC AACTCAAACC ACAGCACCCA CACAACCTCA 
CCTCACCTCG CTCAGACAGG GTCTACAGCT TCTGTTCAGT CTCAAACAGA AGAAGCCAGC 
CAGCACTTGA CGGTGCCCAG CGATCTGTTC AGACCCATCA GTCCTCACAG TTTATCAGAC 
TCAGAGAGCA TCCCTCGAAT TCCCCCACCT CGGCGGGCAC ACACTCTCTC ACGCACACTC 
CGCAGACAGG TGGCAGTGAG TGCTGACTCT CAGGAGACGC TTTACACAGA GGGAGGGGAA 
GCTGAGGGTC CTCTGGGGCT CAGGGAACTG TCGGACCCCC TTCCACCTCA GCAGCAGCAG 
CACCAGCCTT CCCTCTTTCT GGTCCCTGCC ACACCAGGCG CCTCCCCAAA ACCTTCACGA 
CCTAGCGTCC ACACGCAGCA CAACCCCTAC GACCAGTACA ATGTGTTCTC CAGGAGTTCC 
CACTCTCCAC CTGTACCACC ACCACCACCA GACTATAAGA AGCAGGAGGA TGTTGACTCG 
GTGGATCAGG AGGTATCTCG AATTATCCGA GCGGGACTCA CAGGGAGAAG CGATGATGTC 
AACCGAGAAG GGACAGGAGA TCAATGTGCA AAACGTCAGC TTAAGAAATA CCATAGCGTG 
GACACTCAAG GCCAGCGAGC TCTGCTGTTA CCGCGGCCCT TGTCATGGCT TGATGACCCA 
CGCCGGCACT CCATCGAAGT ATGCTCGTCT GTAGAAAGCA GTCCGCAGCG CAGTTCCATA 
TCGTCAGGGT TTGTATCACG AGCCGACAGC CTCCAGCAAC AATCTTCGCC CCGTGGACGC 
AAGAAGAAGA TGAGTCCACC GTGCATTTCT GTAGATCCAC CCGAGGATCC GGAACTTCCG 
GGAGGATTCC ATCCTGCTCT GGGGATTGTG CCACCTCCAT TGCCAAGCCG GGACACTTGC 
CTGAGGAGGC GCGCTCCCTC CAGTGAATCC AAGGACTCGT TTGATTTGGG AGGAGGAGAG 
GCGCTGCCTC AAGAGGGCGT TCCAAACTCC AAGCTATTGA CTCTGCCTAG CTTCTCTTTC 
GAGAAAACAA GCTCTGAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214025.2
CDS	784..8127
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRSKEKELAEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358536.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358536.2

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAGTAAGG AGAAGGAGCT AGCTGAAGCC CAGAGTAAAC CCTTTTACTC CGATTACTGC 
CCAACACGAC GTCTCATCTA CAACATGTGC AAGAGCCAGT ATCTGGACCT GTTCATCACC 
ATCGTCATCG CCCTCAATGT CATCACCATG TCTATGGAGC ACTACCACCA ACCAAAGGTT 
TTGAGCGATT CCCTAAAGAT CTGTAACTAC ATCTTCACAG TCATATTTGT GCTGGAGTCC 
ATCTTCAAAC TTGTTGCTTT TGGATTTCGC CGCTTCTTCA AGGACAGATG GAACCAGTTG 
GATCTGGCCA TTGTTTTGTT GTCTATCATG GGCATAACAC TGGAGGAAAT CGAGGTCAAT 
GCTTCCCTTC CCATCAACCC TACAATCATT CGCATCATGA GGGTGTTGCG CATCACTCGA 
GTTCTAAAGC TACTGAAGAT GGCGGTGGGA ATGCGAGCTC TACTAGACAC TGTGATGCAG 
GCCCTTCCTC AGGTGGGGAA TCTGGGTCTT CTCTTCATGT TGCTTTTCTT CATCTTTGCT 
GCTCTGGGAG TAGAGCTGTT TGGTGACCTG ATATGTGATG AGCTACACCC CTGTGAAGGT 
CTTGGCCGTT ATGCCACGTT CAAGAATTTT GGCATGGCTT TCCTCCTGCT GTTCCGTGTA 
TCCACCGGGG ACAACTGGAA TGGCATCATG AAGGATACGC TCAGAGACTG CTCCCAAGAC 
ACCGGTATAT GCTACAACAC GGTGGTGTCC CCCATTTACT TTGTGTCCTT TGTGCTGACA 
GCTCAGTTTG TGCTGGTCAA TGTGGTGATC GCTGTGCTCA TGAAGCACCT AGAGGAAAGC 
AACAAAGAGG CCAAGGAGGC TGAAGCAGAG GCCGAGCTGG AGCTGGAGCA GGAGATGGAA 
GCTTTGGGTA GAGATAGAGA AATGCAGAGA GGGCACATGC CACGTTTGGG TTCTGGCGAT 
TTGGGTGGGC CAGGAGGGTC ACCTTGGATA TCAAGAGACC ATCGAGACCT GTCAGGACCA 
CTTTACCCCA TAGACAGCCC AACAGCAGAC ATACGCAGAG ATTTTGAAGA TCATTTAAGG 
ACAGATCCTG ATCCACCTCC GAATCTGGAG CCACCATTAG AGCGGAGGCC TATGTTTGAC 
TCAGTTTCCC TGGTGATCCA GGGCTCGCTG GAAGGAGAGC TCAGCCTCAT GGACAACCTG 
TCTGGCTCCA TCTGCCACTA CTACGCTCTG CCCCCCCTTC CCAACAAACA CTGCACTGAG 
AAACAGATTC CTCTAGCCGA GATGGAGGCT CTATCTCTGG CATCGGAGAA ATCCTGGTCC 
TTAGCTCTGA CGGATGACTC TGTCCCAGAT GATTTTAACC CCCTCTTACT AACCAGTCAG 
GAGTGCAATA CCACAGATCC TTCAGATCCA CCAGACACCC GGCAGGCGGA TGAACCGACG 
GATGAACACT TGCTATCCGT GAAGAAGACC TCAGTGGGCC GCACACATTC TCTACCTAAT 
GACAGCTACA TGTTCCTGCC GCTGCAGTCC AACTCAAACC ACAGCACCCA CACAACCTCA 
CCTCACCTCG CTCAGACAGG GTCTACAGCT TCTGTTCAGT CTCAAACAGA AGAAGCCAGC 
CAGCACTTGA CGGTGCCCAG CGATCTGTTC AGACCCATCA GTCCTCACAG TTTATCAGAC 
TCAGAGAGCA TCCCTCGAAT TCCCCCACCT CGGCGGGCAC ACACTCTCTC ACGCACACTC 
CGCAGACAGG TGGCAGTGAG TGCTGACTCT CAGGAGACGC TTTACACAGA GGGAGGGGAA 
GCTGAGGGTC CTCTGGGGCT CAGGGAACTG TCGGACCCCC TTCCACCTCA GCAGCAGCAG 
CACCAGCCTT CCCTCTTTCT GGTCCCTGCC ACACCAGGCG CCTCCCCAAA ACCTTCACGA 
CCTAGCGTCC ACACGCAGCA CAACCCCTAC GACCAGTACA ATGTGTTCTC CAGGAGTTCC 
CACTCTCCAC CTGTACCACC ACCACCACCA GACTATAAGA AGCAGGAGGA TGTTGACTCG 
GTGGATCAGG AGGTATCTCG AATTATCCGA GCGGGACTCA CAGGGAGAAG CGATGATGTC 
AACCGAGAAG GGACAGGAGA TCAATGTGCA AAACGTCAGC TTAAGAAATA CCATAGCGTG 
GACACTCAAG GCCAGCGAGC TCTGCTGTTA CCGCGGCCCT TGTCATGGCT TGATGACCCA 
CGCCGGCACT CCATCGAAGT ATGCTCGTCT GTAGAAAGCA GTCCGCAGCG CAGTTCCATA 
TCGTCAGGGT TTGTATCACG AGCCGACAGC CTCCAGCAAC AATCTTCGCC CCGTGGACGC 
AAGAAGAAGA TGAGTCCACC GTGCATTTCT GTAGATCCAC CCGAGGATCC GGAACTTCCG 
GGAGGATTCC ATCCTGCTCT GGGGATTGTG CCACCTCCAT TGCCAAGCCG GGACACTTGC 
CTGAGGAGGC GCGCTCCCTC CAGTGAATCC AAGGACTCGT TTGATTTGGG AGGAGGAGAG 
GCGCTGCCTC AAGAGGGCGT TCCAAACTCC AAGCTATTGA CTCTGCCTAG CTTCTCTTTC 
GAGAAAACAA GCTCTGAGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480348.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336023.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA.

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CloneID	ODa148367
Clone ID Related Accession (Same CDS sequence)	XM_021480348.1
Accession Version	XM_021480348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAAAGCCC AGAGTAAACC CTTTTACTCC GATTACTGCC CAACACGACG TCTCATCTAC 
AACATGTGCA AGAGCCAGTA TCTGGACCTG TTCATCACCA TCGTCATCGC CCTCAATGTC 
ATCACCATGT CTATGGAGCA CTACCACCAA CCAAAGGTTT TGAGCGATTC CCTAAAGATC 
TGTAACTACA TCTTCACAGT CATATTTGTG CTGGAGTCCA TCTTCAAACT TGTTGCTTTT 
GGATTTCGCC GCTTCTTCAA GGACAGATGG AACCAGTTGG ATCTGGCCAT TGTTTTGTTG 
TCTATCATGG GCATAACACT GGAGGAAATC GAGGTCAATG CTTCCCTTCC CATCAACCCT 
ACAATCATTC GCATCATGAG GGTGTTGCGC ATCACTCGAG TTCTAAAGCT ACTGAAGATG 
GCGGTGGGAA TGCGAGCTCT ACTAGACACT GTGATGCAGG CCCTTCCTCA GGTGGGGAAT 
CTGGGTCTTC TCTTCATGTT GCTTTTCTTC ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT 
GGTGACCTGA TATGTGATGA GCTACACCCC TGTGAAGGTC TTGGCCGTTA TGCCACGTTC 
AAGAATTTTG GCATGGCTTT CCTCCTGCTG TTCCGTGTAT CCACCGGGGA CAACTGGAAT 
GGCATCATGA AGGATACGCT CAGAGACTGC TCCCAAGACA CCGGTATATG CTACAACACG 
GTGGTGTCCC CCATTTACTT TGTGTCCTTT GTGCTGACAG CTCAGTTTGT GCTGGTCAAT 
GTGGTGATCG CTGTGCTCAT GAAGCACCTA GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT 
GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG GAGATGGAAG CTTTGGGTAG AGATAGAGAA 
ATGCAGAGAG GGCACATGCC ACGTTTGGGT TCTGGCGATT TGGGTGGGCC AGGAGGGTCA 
CCTTGGATAT CAAGAGACCA TCGAGACCTG TCAGGACCAC TTTACCCCAT AGACAGCCCA 
ACAGCAGACA TACGCAGAGA TTTTGAAGAT CATTTAAGGA CAGATCCTGA TCCACCTCCG 
AATCTGGAGC CACCATTAGA GCGGAGGCCT ATGTTTGACT CAGTTTCCCT GGTGATCCAG 
GGCTCGCTGG AAGGAGAGCT CAGCCTCATG GACAACCTGT CTGGCTCCAT CTGCCACTAC 
TACGCTCTGC CCCCCCTTCC CAACAAACAC TGCACTGAGA AACAGATTCC TCTAGCCGAG 
ATGGAGGCTC TATCTCTGGC ATCGGAGAAA TCCTGGTCCT TAGCTCTGAC GGATGACTCT 
GTCCCAGATG ATTTTAACCC CCTCTTACTA ACCAGTCAGG AGTGCAATAC CACAGATCCT 
TCAGATCCAC CAGACACCCG GCAGGCGGAT GAACCGACGG ATGAACACTT GCTATCCGTG 
AAGAAGACCT CAGTGGGCCG CACACATTCT CTACCTAATG ACAGCTACAT GTTCCTGCCG 
CTGCAGTCCA ACTCAAACCA CAGCACCCAC ACAACCTCAC CTCACCTCGC TCAGACAGGG 
TCTACAGCTT CTGTTCAGTC TCAAACAGAA GAAGCCAGCC AGCACTTGAC GGTGCCCAGC 
GATCTGTTCA GACCCATCAG TCCTCACAGT TTATCAGACT CAGAGAGCAT CCCTCGAATT 
CCCCCACCTC GGCGGGCACA CACTCTCTCA CGCACACTCC GCAGACAGGT GGCAGTGAGT 
GCTGACTCTC AGGAGACGCT TTACACAGAG GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC 
AGGGAACTGT CGGACCCCCT TCCACCTCAG CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG 
GTCCCTGCCA CACCAGGCGC CTCCCCAAAA CCTTCACGAC CTAGCGTCCA CACGCAGCAC 
AACCCCTACG ACCAGTACAA TGTGTTCTCC AGGAGTTCCC ACTCTCCACC TGTACCACCA 
CCACCACCAG ACTATAAGAA GCAGGAGGAT GTTGACTCGG TGGATCAGGA GGTATCTCGA 
ATTATCCGAG CGGGACTCAC AGGGAGAAGC GATGATGTCA ACCGAGAAGG GACAGGAGAT 
CAATGTGCAA AACGTCAGCT TAAGAAATAC CATAGCGTGG ACACTCAAGG CCAGCGAGCT 
CTGCTGTTAC CGCGGCCCTT GTCATGGCTT GATGACCCAC GCCGGCACTC CATCGAAGTA 
TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC AGTTCCATAT CGTCAGGGTT TGTATCACGA 
GCCGACAGCC TCCAGCAACA ATCTTCGCCC CGTGGACGCA AGAAGAAGAT GAGTCCACCG 
TGCATTTCTG TAGATCCACC CGAGGATCCG GAACTTCCGG GAGGATTCCA TCCTGCTCTG 
GGGATTGTGC CACCTCCATT GCCAAGCCGG GACACTTGCC TGAGGAGGCG CGCTCCCTCC 
AGTGAATCCA AGGACTCGTT TGATTTGGGA GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT 
CCAAACTCCA AGCTATTGAC TCTGCCTAGC TTCTCTTTCG AGAAAACAAG CTCTGAGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336023.1
CDS	783..8105
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRKAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480348.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480348.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG GTGATGCCTC AAAGTCGGAC 
TCTGAAGCCG ATATTTATGC ACGGAGCTTG GAGGATGTTT CGGGACACAA GAAAGATATG 
TCTGCATCAT CCGTTGTGCC CATCAATGGC CATGTGGATC TGAAGAGCAG CCTGACACCG 
CCTCTGATCA CCCACACAGC TGCCACACCA ATGCCCATCC CCAAGATATC AGGAGGAGGA 
GATTCAGCAC TGAGCCAAGA GTCCCGCAGA GGCAGCAGTG TCTCCATGGA GCCTAATGGC 
CAAGACAAAT CTCCATCCAG TGCACGGAGC TCCCCTAATG CCCCCTTGAG CTCCGCCAGT 
AGCTGGAACA GCAGACGCTC CAGCTGGAAC AGTCTAGGCC GAGCCCCCAG CCTCAAGAGG 
CAGAAACACC AGTCTGGTGA AAGGCGCTCC TTGTTGTCTG GTGATGGCCA GTCCAGTTCT 
GATGAGGGGG ATGCAGGAGA GAGCATGGGG GGCATGTCTG AGGGTGATGA TGCCTCCCTC 
GCCAGGACGG ACTCGCTGGG CCAGAGGCCT CGACATAGGC GCATGGAGTC CCTGGAGACG 
CGCAGCTCAT TTGATCTCCA TCCAGACACC CTGCAGGTGC CGTACCCCCA CCGCTCCGGC 
AGCATACACA GCGCACGGCC ACCCAACTTC CTCAGCAACG GCAAGACTTC TCCATCTGCA 
GCCACCACAC AGCTCTCTTT GGATGAACAC CACAGTGAAG ACGACAACGC AGACGAGGAA 
GGAAACCTGA GTCGCAGGGC TCGTCTGTAC AGATGGCTGG AGCACAAGCA GCCCGAATGG 
TGTCGGCAGA GGGTCACCTG GTCCCTTTAC CTCTTTCCTC CAGAGTCACG GTTTCGCGTG 
ACATGCAATA AAATCATCAC CCACAAGATG TTTGACCATG TGGTTCTTGT CATCATCTTT 
CTCAACTGCA TCACCATCGC CATGGAGAGG CCCAGAATCG ACCCCTCTAG CGCTGAACGC 
ATCTTCTTGA CGTTGTCCAA CTACATATTT ACTGCCATCT TTGTGACCGA AATGACAATA 
AAGGTGGTTG CTTTGGGCTT TTGTTTTGGA GAGAAGACCT ACCTGAAGAG CAGCTGGAAC 
ATTCTGGATG GCATGCTGGT GATGATCTCT GTGATTGACA TCCTCGTGTC CCTGATCTCC 
AACAGTGGCA CCAAGATCCT GGGCATGTTA CGTGTGCTGC GGTTACTGAG GACACTCAGG 
CCACTCAGAG TGATCAGCAG AGCTCCTGGA CTGAAACTGG TTGTTGAGAC ATTGATGTCG 
TCTCTTAAGC CTATCGGAAA CATTGTGGTC ATCTGCTGTG CCTTCTTCAT TATTTTTGGG 
ATCTTGGGAG TTCAGCTTTT CAAGGGAAAG TTCTTTGTGT GTCATGGGGA TGATACGAGA 
AACATCACCA ACAAGTCAGA TTGCCTCCTG GCAAACTACA AATGGGTCAG ACACAAGTAC 
AACTTTGATA ACCTTGGACA GGCCCTCATG TCCCTTTTTG TTCTGGCTTC CAAAGACGGT 
TGGGTGGATA TCATGTACGA TGGACTGGAT GCTGTCGGTG TGGACCAGCA GCCCGTCATG 
AATTACAACC CATGGATGCT GCTCTATTTT ATCTCCTTCT TGCTGATCGT GGCTTTCTTC 
GTGCTCAACA TGTTTGTCGG CGTGGTCGTC GAGAACTTTC ACAAGTGCAG GAGAAACCAG 
GAGGAGGAAG AAGCCAAACG GCGAGAAGCG AAGCGCCAGA AACGACGTGA TAAAAAACGA 
AGGAAAGCCC AGAGTAAACC CTTTTACTCC GATTACTGCC CAACACGACG TCTCATCTAC 
AACATGTGCA AGAGCCAGTA TCTGGACCTG TTCATCACCA TCGTCATCGC CCTCAATGTC 
ATCACCATGT CTATGGAGCA CTACCACCAA CCAAAGGTTT TGAGCGATTC CCTAAAGATC 
TGTAACTACA TCTTCACAGT CATATTTGTG CTGGAGTCCA TCTTCAAACT TGTTGCTTTT 
GGATTTCGCC GCTTCTTCAA GGACAGATGG AACCAGTTGG ATCTGGCCAT TGTTTTGTTG 
TCTATCATGG GCATAACACT GGAGGAAATC GAGGTCAATG CTTCCCTTCC CATCAACCCT 
ACAATCATTC GCATCATGAG GGTGTTGCGC ATCACTCGAG TTCTAAAGCT ACTGAAGATG 
GCGGTGGGAA TGCGAGCTCT ACTAGACACT GTGATGCAGG CCCTTCCTCA GGTGGGGAAT 
CTGGGTCTTC TCTTCATGTT GCTTTTCTTC ATCTTTGCTG CTCTGGGAGT AGAGCTGTTT 
GGTGACCTGA TATGTGATGA GCTACACCCC TGTGAAGGTC TTGGCCGTTA TGCCACGTTC 
AAGAATTTTG GCATGGCTTT CCTCCTGCTG TTCCGTGTAT CCACCGGGGA CAACTGGAAT 
GGCATCATGA AGGATACGCT CAGAGACTGC TCCCAAGACA CCGGTATATG CTACAACACG 
GTGGTGTCCC CCATTTACTT TGTGTCCTTT GTGCTGACAG CTCAGTTTGT GCTGGTCAAT 
GTGGTGATCG CTGTGCTCAT GAAGCACCTA GAGGAAAGCA ACAAAGAGGC CAAGGAGGCT 
GAAGCAGAGG CCGAGCTGGA GCTGGAGCAG GAGATGGAAG CTTTGGGTAG AGATAGAGAA 
ATGCAGAGAG GGCACATGCC ACGTTTGGGT TCTGGCGATT TGGGTGGGCC AGGAGGGTCA 
CCTTGGATAT CAAGAGACCA TCGAGACCTG TCAGGACCAC TTTACCCCAT AGACAGCCCA 
ACAGCAGACA TACGCAGAGA TTTTGAAGAT CATTTAAGGA CAGATCCTGA TCCACCTCCG 
AATCTGGAGC CACCATTAGA GCGGAGGCCT ATGTTTGACT CAGTTTCCCT GGTGATCCAG 
GGCTCGCTGG AAGGAGAGCT CAGCCTCATG GACAACCTGT CTGGCTCCAT CTGCCACTAC 
TACGCTCTGC CCCCCCTTCC CAACAAACAC TGCACTGAGA AACAGATTCC TCTAGCCGAG 
ATGGAGGCTC TATCTCTGGC ATCGGAGAAA TCCTGGTCCT TAGCTCTGAC GGATGACTCT 
GTCCCAGATG ATTTTAACCC CCTCTTACTA ACCAGTCAGG AGTGCAATAC CACAGATCCT 
TCAGATCCAC CAGACACCCG GCAGGCGGAT GAACCGACGG ATGAACACTT GCTATCCGTG 
AAGAAGACCT CAGTGGGCCG CACACATTCT CTACCTAATG ACAGCTACAT GTTCCTGCCG 
CTGCAGTCCA ACTCAAACCA CAGCACCCAC ACAACCTCAC CTCACCTCGC TCAGACAGGG 
TCTACAGCTT CTGTTCAGTC TCAAACAGAA GAAGCCAGCC AGCACTTGAC GGTGCCCAGC 
GATCTGTTCA GACCCATCAG TCCTCACAGT TTATCAGACT CAGAGAGCAT CCCTCGAATT 
CCCCCACCTC GGCGGGCACA CACTCTCTCA CGCACACTCC GCAGACAGGT GGCAGTGAGT 
GCTGACTCTC AGGAGACGCT TTACACAGAG GGAGGGGAAG CTGAGGGTCC TCTGGGGCTC 
AGGGAACTGT CGGACCCCCT TCCACCTCAG CAGCAGCAGC ACCAGCCTTC CCTCTTTCTG 
GTCCCTGCCA CACCAGGCGC CTCCCCAAAA CCTTCACGAC CTAGCGTCCA CACGCAGCAC 
AACCCCTACG ACCAGTACAA TGTGTTCTCC AGGAGTTCCC ACTCTCCACC TGTACCACCA 
CCACCACCAG ACTATAAGAA GCAGGAGGAT GTTGACTCGG TGGATCAGGA GGTATCTCGA 
ATTATCCGAG CGGGACTCAC AGGGAGAAGC GATGATGTCA ACCGAGAAGG GACAGGAGAT 
CAATGTGCAA AACGTCAGCT TAAGAAATAC CATAGCGTGG ACACTCAAGG CCAGCGAGCT 
CTGCTGTTAC CGCGGCCCTT GTCATGGCTT GATGACCCAC GCCGGCACTC CATCGAAGTA 
TGCTCGTCTG TAGAAAGCAG TCCGCAGCGC AGTTCCATAT CGTCAGGGTT TGTATCACGA 
GCCGACAGCC TCCAGCAACA ATCTTCGCCC CGTGGACGCA AGAAGAAGAT GAGTCCACCG 
TGCATTTCTG TAGATCCACC CGAGGATCCG GAACTTCCGG GAGGATTCCA TCCTGCTCTG 
GGGATTGTGC CACCTCCATT GCCAAGCCGG GACACTTGCC TGAGGAGGCG CGCTCCCTCC 
AGTGAATCCA AGGACTCGTT TGATTTGGGA GGAGGAGAGG CGCTGCCTCA AGAGGGCGTT 
CCAAACTCCA AGCTATTGAC TCTGCCTAGC TTCTCTTTCG AGAAAACAAG CTCTGAGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_021480349.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336024.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA.

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CloneID	ODa148368
Clone ID Related Accession (Same CDS sequence)	XM_021480349.1
Accession Version	XM_021480349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG ACCACAGATC CTTCAGATCC ACCAGACACC 
CGGCAGGCGG ATGAACCGAC GGATGAACAC TTGCTATCCG TGAAGAAGAC CTCAGTGGGC 
CGCACACATT CTCTACCTAA TGACAGCTAC ATGTTCCTGC CGCTGCAGTC CAACTCAAAC 
CACAGCACCC ACACAACCTC ACCTCACCTC GCTCAGACAG GGTCTACAGC TTCTGTTCAG 
TCTCAAACAG AAGAAGCCAG CCAGCACTTG ACGGTGCCCA GCGATCTGTT CAGACCCATC 
AGTCCTCACA GTTTATCAGA CTCAGAGAGC ATCCCTCGAA TTCCCCCACC TCGGCGGGCA 
CACACTCTCT CACGCACACT CCGCAGACAG GTGGCAGTGA GTGCTGACTC TCAGGAGACG 
CTTTACACAG AGGGAGGGGA AGCTGAGGGT CCTCTGGGGC TCAGGGAACT GTCGGACCCC 
CTTCCACCTC AGCAGCAGCA GCACCAGCCT TCCCTCTTTC TGGTCCCTGC CACACCAGGC 
GCCTCCCCAA AACCTTCACG ACCTAGCGTC CACACGCAGC ACAACCCCTA CGACCAGTAC 
AATGTGTTCT CCAGGAGTTC CCACTCTCCA CCTGTACCAC CACCACCACC AGACTATAAG 
AAGCAGGAGG ATGTTGACTC GGTGGATCAG GAGGTATCTC GAATTATCCG AGCGGGACTC 
ACAGGGAGAA GCGATGATGT CAACCGAGAA GGGACAGGAG ATCAATGTGC AAAACGTCAG 
CTTAAGAAAT ACCATAGCGT GGACACTCAA GGCCAGCGAG CTCTGCTGTT ACCGCGGCCC 
TTGTCATGGC TTGATGACCC ACGCCGGCAC TCCATCGAAG TATGCTCGTC TGTAGAAAGC 
AGTCCGCAGC GCAGTTCCAT ATCGTCAGGG TTTGTATCAC GAGCCGACAG CCTCCAGCAA 
CAATCTTCGC CCCGTGGACG CAAGAAGAAG ATGAGTCCAC CGTGCATTTC TGTAGATCCA 
CCCGAGGATC CGGAACTTCC GGGAGGATTC CATCCTGCTC TGGGGATTGT GCCACCTCCA 
TTGCCAAGCC GGGACACTTG CCTGAGGAGG CGCGCTCCCT CCAGTGAATC CAAGGACTCG 
TTTGATTTGG GAGGAGGAGA GGCGCTGCCT CAAGAGGGCG TTCCAAACTC CAAGCTATTG 
ACTCTGCCTA GCTTCTCTTT CGAGAAAACA AGCTCTGAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336024.1
CDS	783..8027
Translation	MMAEDGGIISEDNQLNEAEGSSSLPRTFIPLNDLSGGGVRSELESDGGERRESAAGGAAEETSASDGEALPYPSLAPVVFFYLKQTTRPRSWCLKMVCNPWFERASMLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGKGHVGVRIVKPNPDTMSTVLSSDLAGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRNIMLTGVSWSSPDHTQEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLETTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_021480349.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480349.1

ORF Insert Sequence:

ATGATGGCTG AAGATGGAGG AATTATCTCC GAGGACAATC AGCTGAACGA AGCAGAGGGA 
AGCTCCTCGC TTCCAAGGAC TTTTATACCA CTTAATGACC TGTCCGGTGG CGGTGTGCGC 
AGTGAGCTCG AGTCTGATGG AGGTGAAAGG CGGGAGAGCG CAGCCGGAGG AGCGGCAGAG 
GAGACGTCCG CTAGCGACGG TGAAGCTTTG CCCTATCCTT CCTTGGCACC GGTTGTGTTT 
TTCTATCTGA AGCAGACCAC GCGTCCAAGG AGTTGGTGCC TGAAAATGGT TTGCAATCCG 
TGGTTCGAGA GGGCCAGTAT GCTGGTGATC CTGCTGAACT GTGTTACTCT GGGGATGTTT 
CACCCGTGCG AGGACAGCCA CTGTGACTCA GAGCGATGCA AGATACTGGA GGACTTTGAT 
GATTTCATCT TTGCTTTTTT TGCCGTGGAG ATGGTAATCA AGATGGTTGC ACTGGGTATC 
TTTGGGAAAA AGTGTTACCT TGGAGACACC TGGAATCGGC TAGACTTCTT CATTGTGTTG 
GCTGGGATGC TGGAATATTC TCTCAACCTG CAGAATGTTA GTTTCTCTGC GGTCCGAACA 
GTCAGAGTAC TTCGACCTCT TCGGGCTATC AACCGAGTTC CCAGTATGAG AATCCTGGTG 
ACGCTTCTAC TTGACACTCT CCCCATGCTG GGCAATGTGC TGCTGCTTTG CTTCTTTGTT 
TTCTTCATCT TTGGGATCGT TGGAGTTCAG CTGTGGGCCG GGCTTCTCCG CAACCGCTGC 
TTCTTACCTG AAAACTTCTC CCTACCTACC AGCCTAGAAC TTCGCAAGTA CTACCACACA 
GAAAACGACG ATGAAAACCC GTTTATCTGC TCCCAACCAA GGGAGAACGG CATGCGTCTG 
TGCACCTCCG TCCCCACGCT CCATGAGGAG GGCCGGCAGT GCCAGCTGGA CATGGCATCT 
TACAATAGCA CAGACAACAC CACCTGCGTC AACTGGAACA AGTATTACAC CAACTGCTCC 
GCCGGAGAGG CCAATCCCTT CAAGGGGGCC ATAAACTTTG ATAACATTGG ATATGCCTGG 
ATTGCCATCT TTCAGGTTAT TACTCTGGAG GGCTGGGTGG ACATCATGTA CTTTGTTATG 
GATGCTCACT CCTTCTACAA TTTCATATAC TTCATCCTCC TGATAATAGT GGGCTCGTTC 
TTCATGATCA ACCTTTGTCT AGTTGTCATA GCGACACAGT TCTCCGAGAC CAAGCAACGA 
GAGAGTCAGC TGATGAAGGA GCAGCGAGTT CGCTTTAGGT CCAACGCCAG CACTCTCAAC 
AGCTACTCCG AGCCTGGAAG CTGCTATGAT GAGCTGCTCA AATACCTGGT CCACGTCATC 
CGCAAAGGCA CCAGACAAAT CGCTCACCTC ATCCGGGCTG CGGGTCGCCG TGCAGGCCTG 
AGGATCTGTG CTTCTCCACC TCTGGAAGCA CCACCCACTA AAAGACGTCG GCAAAAGCAG 
CGTCAGGGAT CTATTCGTCA CATCATGCAC CATCACCACC ACCACCTCCA CCATCACTAT 
CATTTGGGCA ATGGGAGTGT CCGATCTGAC GGGGGACGAG AGGTGGACAA TCCTTCGCAG 
AGTGGCATTG TCAACAAAAG TGGCAATGTC AATCTAATGC TACCTGTTAT TGTCCCTCAG 
CAGGACGCCT TTAGTGGGTC TTTTGGCCAC AGTAGTGCCG AATCGGTACA TAGTGTTTAT 
CAGGTCTCGG GTCACCTTGA GCCACTTCGC TGTGGCCCTT CACCGTCTCC CACAGTTCTT 
CATGCGTATA AGAGGAACTC AGTGCCCTTT GCAGCTCCAG TACATAAGAA CTACCCCACT 
TTACAGTCAT CTCTGGCCTT AGAGCAGCTC AGACAGAGAA TCCTGGATCC TGCTAGTGTC 
CTTACCAGCC TGAACATACC ACCAAATCCC ATCAACACCT CACAGTGCCT GGTGGAGACT 
CAAGGTCCTC CTGGCAAGGT CATCTCAAAG GATGCCCTCA TGAACTGCAG TAGCACAAAC 
ATAGACACAG CTCTGACCTA TGACCCTGAA ACCTGTCCAT ATTGTGCCAA ATCAATGGCC 
AATGACTCTG AGGGAATAGA TGGTAATGAG ACGGCAGACT CTGACAGTGA GGGTGTCTAT 
GAGACACAAG TTGCCCATTA TCGGGACAGC AGAGGACCCA AGAAGAAGAA AAAGTTTGCA 
TTAGGAGCTC GGGCAACAAA GGTGATCGAT TTCTGGAGAC TGGTCTGTGG CACTTTCCGC 
AAGATTGTGG ACAGCAAGTA TTTTGGACAA GGAATAATGA TCGCCATTCT TATTAACACA 
CTCAGCATGG GCATTGAGTA CCATGAACAG CCTGATGAGT TGACAAATGC TCTGGAAATA 
AGCAACATCG TCTTCACCAG TTTGTTTGTG CTGGAGATGC TCCTGAAACT GCTAGTCTAT 
GGGCCTTTTG GGTACATCAA GAACCCGTAC AACATCTTTG ACGGCATTAT TGTTGTAATC 
AGTGTTTGGG AGATTGTTGG CCAGCAGGGA GGAGGTTTAT CGGTGCTGAG GACCTTCAGG 
CTGATGCGGG TGTTGAAGTT GGTCCGATTT ATGCCCGCCT TGCAGAGGCA GTTGGTGGTC 
CTCATGAAGA CCATGGACAA TGTTGCCACC TTCTGCATGC TTCTGATGCT TTTCATATTC 
ATTTTCAGCA TTCTAGGCAT GCACTTGTTT GGGTGTAAAT TCGGCTCTGA GAGAGATGGA 
GACACACTTC CCGACCGAAA GAACTTTGAT TCTTTATTGT GGGCCATTGT TACAGTTTTT 
CAGATCCTGA CTCAGGAGGA CTGGAATAAA GTGCTGTACA ACGGGATGGC CTCCACCTCT 
CCTGTGGCTG CTCTGTACTT CATCGCCCTC ATGACCTTTG GGAATTATGT CTTGTTTAAC 
CTCTTAGTTG CTATCTTAGT GGAGGGATTC CAAACAGAGG AAATTACCAA GCGGGACGAT 
TTGCATGGCC AGCTGAGCTG TATTCAGCTG CCTATAGACT CAGGGAAAGG CCATGTTGGA 
GTGAGGATAG TCAAGCCCAA TCCAGACACA ATGTCCACAG TGCTCAGTTC TGATCTTGCA 
GGTGATGCCT CAAAGTCGGA CTCTGAAGCC GATATTTATG CACGGAGCTT GGAGGATGTT 
TCGGGACACA AGAAAGATAT GTCTGCATCA TCCGTTGTGC CCATCAATGG CCATGTGGAT 
CTGAAGAGCA GCCTGACACC GCCTCTGATC ACCCACACAG CTGCCACACC AATGCCCATC 
CCCAAGATAT CAGGAGGAGG AGATTCAGCA CTGAGCCAAG AGTCCCGCAG AGGCAGCAGT 
GTCTCCATGG AGCCTAATGG CCAAGACAAA TCTCCATCCA GTGCACGGAG CTCCCCTAAT 
GCCCCCTTGA GCTCCGCCAG TAGCTGGAAC AGCAGACGCT CCAGCTGGAA CAGTCTAGGC 
CGAGCCCCCA GCCTCAAGAG GCAGAAACAC CAGTCTGGTG AAAGGCGCTC CTTGTTGTCT 
GGTGATGGCC AGTCCAGTTC TGATGAGGGG GATGCAGGAG AGAGCATGGG GGGCATGTCT 
GAGGGTGATG ATGCCTCCCT CGCCAGGACG GACTCGCTGG GCCAGAGGCC TCGACATAGG 
CGCATGGAGT CCCTGGAGAC GCGCAGCTCA TTTGATCTCC ATCCAGACAC CCTGCAGGTG 
CCGTACCCCC ACCGCTCCGG CAGCATACAC AGCGCACGGC CACCCAACTT CCTCAGCAAC 
GGCAAGACTT CTCCATCTGC AGCCACCACA CAGCTCTCTT TGGATGAACA CCACAGTGAA 
GACGACAACG CAGACGAGGA AGGAAACCTG AGTCGCAGGG CTCGTCTGTA CAGATGGCTG 
GAGCACAAGC AGCCCGAATG GTGTCGGCAG AGGGTCACCT GGTCCCTTTA CCTCTTTCCT 
CCAGAGTCAC GGTTTCGCGT GACATGCAAT AAAATCATCA CCCACAAGAT GTTTGACCAT 
GTGGTTCTTG TCATCATCTT TCTCAACTGC ATCACCATCG CCATGGAGAG GCCCAGAATC 
GACCCCTCTA GCGCTGAACG CATCTTCTTG ACGTTGTCCA ACTACATATT TACTGCCATC 
TTTGTGACCG AAATGACAAT AAAGGTGGTT GCTTTGGGCT TTTGTTTTGG AGAGAAGACC 
TACCTGAAGA GCAGCTGGAA CATTCTGGAT GGCATGCTGG TGATGATCTC TGTGATTGAC 
ATCCTCGTGT CCCTGATCTC CAACAGTGGC ACCAAGATCC TGGGCATGTT ACGTGTGCTG 
CGGTTACTGA GGACACTCAG GCCACTCAGA GTGATCAGCA GAGCTCCTGG ACTGAAACTG 
GTTGTTGAGA CATTGATGTC GTCTCTTAAG CCTATCGGAA ACATTGTGGT CATCTGCTGT 
GCCTTCTTCA TTATTTTTGG GATCTTGGGA GTTCAGCTTT TCAAGGGAAA GTTCTTTGTG 
TGTCATGGGG ATGATACGAG AAACATCACC AACAAGTCAG ATTGCCTCCT GGCAAACTAC 
AAATGGGTCA GACACAAGTA CAACTTTGAT AACCTTGGAC AGGCCCTCAT GTCCCTTTTT 
GTTCTGGCTT CCAAAGACGG TTGGGTGGAT ATCATGTACG ATGGACTGGA TGCTGTCGGT 
GTGGACCAGC AGCCCGTCAT GAATTACAAC CCATGGATGC TGCTCTATTT TATCTCCTTC 
TTGCTGATCG TGGCTTTCTT CGTGCTCAAC ATGTTTGTCG GCGTGGTCGT CGAGAACTTT 
CACAAGTGCA GGAGAAACCA GGAGGAGGAA GAAGCCAAAC GGCGAGAAGC GAAGCGCCAG 
AAACGACGTG ATAAAAAACG AAGGAATATA ATGCTGACCG GTGTCAGTTG GTCCAGTCCT 
GATCACACAC AAGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAATGC TTCCCTTCCC 
ATCAACCCTA CAATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AGAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG ACCACAGATC CTTCAGATCC ACCAGACACC 
CGGCAGGCGG ATGAACCGAC GGATGAACAC TTGCTATCCG TGAAGAAGAC CTCAGTGGGC 
CGCACACATT CTCTACCTAA TGACAGCTAC ATGTTCCTGC CGCTGCAGTC CAACTCAAAC 
CACAGCACCC ACACAACCTC ACCTCACCTC GCTCAGACAG GGTCTACAGC TTCTGTTCAG 
TCTCAAACAG AAGAAGCCAG CCAGCACTTG ACGGTGCCCA GCGATCTGTT CAGACCCATC 
AGTCCTCACA GTTTATCAGA CTCAGAGAGC ATCCCTCGAA TTCCCCCACC TCGGCGGGCA 
CACACTCTCT CACGCACACT CCGCAGACAG GTGGCAGTGA GTGCTGACTC TCAGGAGACG 
CTTTACACAG AGGGAGGGGA AGCTGAGGGT CCTCTGGGGC TCAGGGAACT GTCGGACCCC 
CTTCCACCTC AGCAGCAGCA GCACCAGCCT TCCCTCTTTC TGGTCCCTGC CACACCAGGC 
GCCTCCCCAA AACCTTCACG ACCTAGCGTC CACACGCAGC ACAACCCCTA CGACCAGTAC 
AATGTGTTCT CCAGGAGTTC CCACTCTCCA CCTGTACCAC CACCACCACC AGACTATAAG 
AAGCAGGAGG ATGTTGACTC GGTGGATCAG GAGGTATCTC GAATTATCCG AGCGGGACTC 
ACAGGGAGAA GCGATGATGT CAACCGAGAA GGGACAGGAG ATCAATGTGC AAAACGTCAG 
CTTAAGAAAT ACCATAGCGT GGACACTCAA GGCCAGCGAG CTCTGCTGTT ACCGCGGCCC 
TTGTCATGGC TTGATGACCC ACGCCGGCAC TCCATCGAAG TATGCTCGTC TGTAGAAAGC 
AGTCCGCAGC GCAGTTCCAT ATCGTCAGGG TTTGTATCAC GAGCCGACAG CCTCCAGCAA 
CAATCTTCGC CCCGTGGACG CAAGAAGAAG ATGAGTCCAC CGTGCATTTC TGTAGATCCA 
CCCGAGGATC CGGAACTTCC GGGAGGATTC CATCCTGCTC TGGGGATTGT GCCACCTCCA 
TTGCCAAGCC GGGACACTTG CCTGAGGAGG CGCGCTCCCT CCAGTGAATC CAAGGACTCG 
TTTGATTTGG GAGGAGGAGA GGCGCTGCCT CAAGAGGGCG TTCCAAACTC CAAGCTATTG 
ACTCTGCCTA GCTTCTCTTT CGAGAAAACA AGCTCTGAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1g ( XM_017358537.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214026.1 Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

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CloneID	ODa54519
Clone ID Related Accession (Same CDS sequence)	XM_017358537.1
Accession Version	XM_017358537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent T-type calcium channel subunit alpha-1G isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTGA TCCTGCTGAA CTGTGTTACT CTGGGGATGT TTCACCCGTG CGAGGACAGC 
CACTGTGACT CAGAGCGATG CAAGATACTG GAGGACTTTG ATGATTTCAT CTTTGCTTTT 
TTTGCCGTGG AGATGGTAAT CAAGATGGTT GCACTGGGTA TCTTTGGGAA AAAGTGTTAC 
CTTGGAGACA CCTGGAATCG GCTAGACTTC TTCATTGTGT TGGCTGGGAT GCTGGAATAT 
TCTCTCAACC TGCAGAATGT TAGTTTCTCT GCGGTCCGAA CAGTCAGAGT ACTTCGACCT 
CTTCGGGCTA TCAACCGAGT TCCCAGTATG AGAATCCTGG TGACGCTTCT ACTTGACACT 
CTCCCCATGC TGGGCAATGT GCTGCTGCTT TGCTTCTTTG TTTTCTTCAT CTTTGGGATC 
GTTGGAGTTC AGCTGTGGGC CGGGCTTCTC CGCAACCGCT GCTTCTTACC TGAAAACTTC 
TCCCTACCTA CCAGCCTAGA ACTTCGCAAG TACTACCACA CAGAAAACGA CGATGAAAAC 
CCGTTTATCT GCTCCCAACC AAGGGAGAAC GGCATGCGTC TGTGCACCTC CGTCCCCACG 
CTCCATGAGG AGGGCCGGCA GTGCCAGCTG GACATGGCAT CTTACAATAG CACAGACAAC 
ACCACCTGCG TCAACTGGAA CAAGTATTAC ACCAACTGCT CCGCCGGAGA GGCCAATCCC 
TTCAAGGGGG CCATAAACTT TGATAACATT GGATATGCCT GGATTGCCAT CTTTCAGGTT 
ATTACTCTGG AGGGCTGGGT GGACATCATG TACTTTGTTA TGGATGCTCA CTCCTTCTAC 
AATTTCATAT ACTTCATCCT CCTGATAATA GTGGGCTCGT TCTTCATGAT CAACCTTTGT 
CTAGTTGTCA TAGCGACACA GTTCTCCGAG ACCAAGCAAC GAGAGAGTCA GCTGATGAAG 
GAGCAGCGAG TTCGCTTTAG GTCCAACGCC AGCACTCTCA ACAGCTACTC CGAGCCTGGA 
AGCTGCTATG ATGAGCTGCT CAAATACCTG GTCCACGTCA TCCGCAAAGG CACCAGACAA 
ATCGCTCACC TCATCCGGGC TGCGGGTCGC CGTGCAGGCC TGAGGATCTG TGCTTCTCCA 
CCTCTGGAAG CACCACCCAC TAAAAGACGT CGGCAAAAGC AGCGTCAGGG ATCTATTCGT 
CACATCATGC ACCATCACCA CCACCACCTC CACCATCACT ATCATTTGGG CAATGGGAGT 
GTCCGATCTG ACGGGGGACG AGAGGTGGAC AATCCTTCGC AGAGTGGCAT TGTCAACAAA 
AGTGGCAATG TCAATCTAAT GCTACCTGTT ATTGTCCCTC AGCAGGACGC CTTTAGTGGG 
TCTTTTGGCC ACAGTAGTGC CGAATCGGTA CATAGTGTTT ATCAGGTCTC GGGTCACCTT 
GAGCCACTTC GCTGTGGCCC TTCACCGTCT CCCACAGTTC TTCATGCGTA TAAGAGGAAC 
TCAGTGCCCT TTGCAGCTCC AGTACATAAG AACTACCCCA CTTTACAGTC ATCTCTGGCC 
TTAGAGCAGC TCAGACAGAG AATCCTGGAT CCTGCTAGTG TCCTTACCAG CCTGAACATA 
CCACCAAATC CCATCAACAC CTCACAGTGC CTGGTGGAGA CTCAAGGTCC TCCTGGCAAG 
GTCATCTCAA AGGATGCCCT CATGAACTGC AGTAGCACAA ACATAGACAC AGCTCTGACC 
TATGACCCTG AAACCTGTCC ATATTGTGCC AAATCAATGG CCAATGACTC TGAGGGAATA 
GATGGTAATG AGACGGCAGA CTCTGACAGT GAGGGTGTCT ATGAGACACA AGTTGCCCAT 
TATCGGGACA GCAGAGGACC CAAGAAGAAG AAAAAGTTTG CATTAGGAGC TCGGGCAACA 
AAGGTGATCG ATTTCTGGAG ACTGGTCTGT GGCACTTTCC GCAAGATTGT GGACAGCAAG 
TATTTTGGAC AAGGAATAAT GATCGCCATT CTTATTAACA CACTCAGCAT GGGCATTGAG 
TACCATGAAC AGCCTGATGA GTTGACAAAT GCTCTGGAAA TAAGCAACAT CGTCTTCACC 
AGTTTGTTTG TGCTGGAGAT GCTCCTGAAA CTGCTAGTCT ATGGGCCTTT TGGGTACATC 
AAGAACCCGT ACAACATCTT TGACGGCATT ATTGTTGTAA TCAGTGTTTG GGAGATTGTT 
GGCCAGCAGG GAGGAGGTTT ATCGGTGCTG AGGACCTTCA GGCTGATGCG GGTGTTGAAG 
TTGGTCCGAT TTATGCCCGC CTTGCAGAGG CAGTTGGTGG TCCTCATGAA GACCATGGAC 
AATGTTGCCA CCTTCTGCAT GCTTCTGATG CTTTTCATAT TCATTTTCAG CATTCTAGGC 
ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGAAATTACC AAGCGGGACG ATTTGCATGG CCAGCTGAGC 
TGTATTCAGC TGCCTATAGA CTCAGGGGGT GATGCCTCAA AGTCGGACTC TGAAGCCGAT 
ATTTATGCAC GGAGCTTGGA GGATGTTTCG GGACACAAGA AAGATATGTC TGCATCATCC 
GTTGTGCCCA TCAATGGCCA TGTGGATCTG AAGAGCAGCC TGACACCGCC TCTGATCACC 
CACACAGCTG CCACACCAAT GCCCATCCCC AAGATATCAG GAGGAGGAGA TTCAGCACTG 
AGCCAAGAGT CCCGCAGAGG CAGCAGTGTC TCCATGGAGC CTAATGGCCA AGACAAATCT 
CCATCCAGTG CACGGAGCTC CCCTAATGCC CCCTTGAGCT CCGCCAGTAG CTGGAACAGC 
AGACGCTCCA GCTGGAACAG TCTAGGCCGA GCCCCCAGCC TCAAGAGGCA GAAACACCAG 
TCTGGTGAAA GGCGCTCCTT GTTGTCTGGT GATGGCCAGT CCAGTTCTGA TGAGGGGGAT 
GCAGGAGAGA GCATGGGGGG CATGTCTGAG GGTGATGATG CCTCCCTCGC CAGGACGGAC 
TCGCTGGGCC AGAGGCCTCG ACATAGGCGC ATGGAGTCCC TGGAGACGCG CAGCTCATTT 
GATCTCCATC CAGACACCCT GCAGGTGCCG TACCCCCACC GCTCCGGCAG CATACACAGC 
GCACGGCCAC CCAACTTCCT CAGCAACGGC AAGACTTCTC CATCTGCAGC CACCACACAG 
CTCTCTTTGG ATGAACACCA CAGTGAAGAC GACAACGCAG ACGAGGAAGG AAACCTGAGT 
CGCAGGGCTC GTCTGTACAG ATGGCTGGAG CACAAGCAGC CCGAATGGTG TCGGCAGAGG 
GTCACCTGGT CCCTTTACCT CTTTCCTCCA GAGTCACGGT TTCGCGTGAC ATGCAATAAA 
ATCATCACCC ACAAGATGTT TGACCATGTG GTTCTTGTCA TCATCTTTCT CAACTGCATC 
ACCATCGCCA TGGAGAGGCC CAGAATCGAC CCCTCTAGCG CTGAACGCAT CTTCTTGACG 
TTGTCCAACT ACATATTTAC TGCCATCTTT GTGACCGAAA TGACAATAAA GGTGGTTGCT 
TTGGGCTTTT GTTTTGGAGA GAAGACCTAC CTGAAGAGCA GCTGGAACAT TCTGGATGGC 
ATGCTGGTGA TGATCTCTGT GATTGACATC CTCGTGTCCC TGATCTCCAA CAGTGGCACC 
AAGATCCTGG GCATGTTACG TGTGCTGCGG TTACTGAGGA CACTCAGGCC ACTCAGAGTG 
ATCAGCAGAG CTCCTGGACT GAAACTGGTT GTTGAGACAT TGATGTCGTC TCTTAAGCCT 
ATCGGAAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATTA TTTTTGGGAT CTTGGGAGTT 
CAGCTTTTCA AGGGAAAGTT CTTTGTGTGT CATGGGGATG ATACGAGAAA CATCACCAAC 
AAGTCAGATT GCCTCCTGGC AAACTACAAA TGGGTCAGAC ACAAGTACAA CTTTGATAAC 
CTTGGACAGG CCCTCATGTC CCTTTTTGTT CTGGCTTCCA AAGACGGTTG GGTGGATATC 
ATGTACGATG GACTGGATGC TGTCGGTGTG GACCAGCAGC CCGTCATGAA TTACAACCCA 
TGGATGCTGC TCTATTTTAT CTCCTTCTTG CTGATCGTGG CTTTCTTCGT GCTCAACATG 
TTTGTCGGCG TGGTCGTCGA GAACTTTCAC AAGTGCAGGA GAAACCAGGA GGAGGAAGAA 
GCCAAACGGC GAGAAGCGAA GCGCCAGAAA CGACGTGATA AAAAACGAAG GAGTAAGGAG 
AAGGAGCTAG CTGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAACGC TTCCCTTCCC 
ATCAATCCTA CCATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AAAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT 
CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG 
GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC 
ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG 
CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG 
TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT 
CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG 
GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC 
CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG 
GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT 
CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC 
CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC 
ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT 
GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG 
GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG 
ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC 
CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC 
ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT 
GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG 
AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT 
CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC 
GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA 
GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC 
TCTGAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214026.1
CDS	15..7106
Translation	MLVILLNCVTLGMFHPCEDSHCDSERCKILEDFDDFIFAFFAVEMVIKMVALGIFGKKCYLGDTWNRLDFFIVLAGMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPTSLELRKYYHTENDDENPFICSQPRENGMRLCTSVPTLHEEGRQCQLDMASYNSTDNTTCVNWNKYYTNCSAGEANPFKGAINFDNIGYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMKEQRVRFRSNASTLNSYSEPGSCYDELLKYLVHVIRKGTRQIAHLIRAAGRRAGLRICASPPLEAPPTKRRRQKQRQGSIRHIMHHHHHHLHHHYHLGNGSVRSDGGREVDNPSQSGIVNKSGNVNLMLPVIVPQQDAFSGSFGHSSAESVHSVYQVSGHLEPLRCGPSPSPTVLHAYKRNSVPFAAPVHKNYPTLQSSLALEQLRQRILDPASVLTSLNIPPNPINTSQCLVETQGPPGKVISKDALMNCSSTNIDTALTYDPETCPYCAKSMANDSEGIDGNETADSDSEGVYETQVAHYRDSRGPKKKKKFALGARATKVIDFWRLVCGTFRKIVDSKYFGQGIMIAILINTLSMGIEYHEQPDELTNALEISNIVFTSLFVLEMLLKLLVYGPFGYIKNPYNIFDGIIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFMPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFGSERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNKVLYNGMASTSPVAALYFIALMTFGNYVLFNLLVAILVEGFQTEEITKRDDLHGQLSCIQLPIDSGGDASKSDSEADIYARSLEDVSGHKKDMSASSVVPINGHVDLKSSLTPPLITHTAATPMPIPKISGGGDSALSQESRRGSSVSMEPNGQDKSPSSARSSPNAPLSSASSWNSRRSSWNSLGRAPSLKRQKHQSGERRSLLSGDGQSSSDEGDAGESMGGMSEGDDASLARTDSLGQRPRHRRMESLETRSSFDLHPDTLQVPYPHRSGSIHSARPPNFLSNGKTSPSAATTQLSLDEHHSEDDNADEEGNLSRRARLYRWLEHKQPEWCRQRVTWSLYLFPPESRFRVTCNKIITHKMFDHVVLVIIFLNCITIAMERPRIDPSSAERIFLTLSNYIFTAIFVTEMTIKVVALGFCFGEKTYLKSSWNILDGMLVMISVIDILVSLISNSGTKILGMLRVLRLLRTLRPLRVISRAPGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFFVCHGDDTRNITNKSDCLLANYKWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPVMNYNPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRRNQEEEEAKRREAKRQKRRDKKRRSKEKELAEAQSKPFYSDYCPTRRLIYNMCKSQYLDLFITIVIALNVITMSMEHYHQPKVLSDSLKICNYIFTVIFVLESIFKLVAFGFRRFFKDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRITRVLKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLICDELHPCEGLGRYATFKNFGMAFLLLFRVSTGDNWNGIMKDTLRDCSQDTGICYNTVVSPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEAEAEAELELEQEMEALGRDREMQRGHMPRLGSGDLGGPGGSPWISRDHRDLSGPLYPIDSPTADIRRDFEDHLRTDPDPPPNLEPPLERRPMFDSVSLVIQGSLEGELSLMDNLSGSICHYYALPPLPNKHCTEKQIPLAEMEALSLASEKSWSLALTDDSVPDDFNPLLLTSQECNTTDPSDPPDTRQADEPTDEHLLSVKKTSVGRTHSLPNDSYMFLPLQSNSNHSTHTTSPHLAQTGSTASVQSQTEEASQHLTVPSDLFRPISPHSLSDSESIPRIPPPRRAHTLSRTLRRQVAVSADSQETLYTEGGEAEGPLGLRELSDPLPPQQQQHQPSLFLVPATPGASPKPSRPSVHTQHNPYDQYNVFSRSSHSPPVPPPPPDYKKQEDVDSVDQEVSRIIRAGLTGRSDDVNREGTGDQCAKRQLKKYHSVDTQGQRALLLPRPLSWLDDPRRHSIEVCSSVESSPQRSSISSGFVSRADSLQQQSSPRGRKKKMSPPCISVDPPEDPELPGGFHPALGIVPPPLPSRDTCLRRRAPSSESKDSFDLGGGEALPQEGVPNSKLLTLPSFSFEKTSSEH

Target ORF information:

RefSeq Version	XM_017358537.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, T type, alpha 1G subunit (cacna1g), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358537.1

ORF Insert Sequence:

ATGCTGGTGA TCCTGCTGAA CTGTGTTACT CTGGGGATGT TTCACCCGTG CGAGGACAGC 
CACTGTGACT CAGAGCGATG CAAGATACTG GAGGACTTTG ATGATTTCAT CTTTGCTTTT 
TTTGCCGTGG AGATGGTAAT CAAGATGGTT GCACTGGGTA TCTTTGGGAA AAAGTGTTAC 
CTTGGAGACA CCTGGAATCG GCTAGACTTC TTCATTGTGT TGGCTGGGAT GCTGGAATAT 
TCTCTCAACC TGCAGAATGT TAGTTTCTCT GCGGTCCGAA CAGTCAGAGT ACTTCGACCT 
CTTCGGGCTA TCAACCGAGT TCCCAGTATG AGAATCCTGG TGACGCTTCT ACTTGACACT 
CTCCCCATGC TGGGCAATGT GCTGCTGCTT TGCTTCTTTG TTTTCTTCAT CTTTGGGATC 
GTTGGAGTTC AGCTGTGGGC CGGGCTTCTC CGCAACCGCT GCTTCTTACC TGAAAACTTC 
TCCCTACCTA CCAGCCTAGA ACTTCGCAAG TACTACCACA CAGAAAACGA CGATGAAAAC 
CCGTTTATCT GCTCCCAACC AAGGGAGAAC GGCATGCGTC TGTGCACCTC CGTCCCCACG 
CTCCATGAGG AGGGCCGGCA GTGCCAGCTG GACATGGCAT CTTACAATAG CACAGACAAC 
ACCACCTGCG TCAACTGGAA CAAGTATTAC ACCAACTGCT CCGCCGGAGA GGCCAATCCC 
TTCAAGGGGG CCATAAACTT TGATAACATT GGATATGCCT GGATTGCCAT CTTTCAGGTT 
ATTACTCTGG AGGGCTGGGT GGACATCATG TACTTTGTTA TGGATGCTCA CTCCTTCTAC 
AATTTCATAT ACTTCATCCT CCTGATAATA GTGGGCTCGT TCTTCATGAT CAACCTTTGT 
CTAGTTGTCA TAGCGACACA GTTCTCCGAG ACCAAGCAAC GAGAGAGTCA GCTGATGAAG 
GAGCAGCGAG TTCGCTTTAG GTCCAACGCC AGCACTCTCA ACAGCTACTC CGAGCCTGGA 
AGCTGCTATG ATGAGCTGCT CAAATACCTG GTCCACGTCA TCCGCAAAGG CACCAGACAA 
ATCGCTCACC TCATCCGGGC TGCGGGTCGC CGTGCAGGCC TGAGGATCTG TGCTTCTCCA 
CCTCTGGAAG CACCACCCAC TAAAAGACGT CGGCAAAAGC AGCGTCAGGG ATCTATTCGT 
CACATCATGC ACCATCACCA CCACCACCTC CACCATCACT ATCATTTGGG CAATGGGAGT 
GTCCGATCTG ACGGGGGACG AGAGGTGGAC AATCCTTCGC AGAGTGGCAT TGTCAACAAA 
AGTGGCAATG TCAATCTAAT GCTACCTGTT ATTGTCCCTC AGCAGGACGC CTTTAGTGGG 
TCTTTTGGCC ACAGTAGTGC CGAATCGGTA CATAGTGTTT ATCAGGTCTC GGGTCACCTT 
GAGCCACTTC GCTGTGGCCC TTCACCGTCT CCCACAGTTC TTCATGCGTA TAAGAGGAAC 
TCAGTGCCCT TTGCAGCTCC AGTACATAAG AACTACCCCA CTTTACAGTC ATCTCTGGCC 
TTAGAGCAGC TCAGACAGAG AATCCTGGAT CCTGCTAGTG TCCTTACCAG CCTGAACATA 
CCACCAAATC CCATCAACAC CTCACAGTGC CTGGTGGAGA CTCAAGGTCC TCCTGGCAAG 
GTCATCTCAA AGGATGCCCT CATGAACTGC AGTAGCACAA ACATAGACAC AGCTCTGACC 
TATGACCCTG AAACCTGTCC ATATTGTGCC AAATCAATGG CCAATGACTC TGAGGGAATA 
GATGGTAATG AGACGGCAGA CTCTGACAGT GAGGGTGTCT ATGAGACACA AGTTGCCCAT 
TATCGGGACA GCAGAGGACC CAAGAAGAAG AAAAAGTTTG CATTAGGAGC TCGGGCAACA 
AAGGTGATCG ATTTCTGGAG ACTGGTCTGT GGCACTTTCC GCAAGATTGT GGACAGCAAG 
TATTTTGGAC AAGGAATAAT GATCGCCATT CTTATTAACA CACTCAGCAT GGGCATTGAG 
TACCATGAAC AGCCTGATGA GTTGACAAAT GCTCTGGAAA TAAGCAACAT CGTCTTCACC 
AGTTTGTTTG TGCTGGAGAT GCTCCTGAAA CTGCTAGTCT ATGGGCCTTT TGGGTACATC 
AAGAACCCGT ACAACATCTT TGACGGCATT ATTGTTGTAA TCAGTGTTTG GGAGATTGTT 
GGCCAGCAGG GAGGAGGTTT ATCGGTGCTG AGGACCTTCA GGCTGATGCG GGTGTTGAAG 
TTGGTCCGAT TTATGCCCGC CTTGCAGAGG CAGTTGGTGG TCCTCATGAA GACCATGGAC 
AATGTTGCCA CCTTCTGCAT GCTTCTGATG CTTTTCATAT TCATTTTCAG CATTCTAGGC 
ATGCACTTGT TTGGGTGTAA ATTCGGCTCT GAGAGAGATG GAGACACACT TCCCGACCGA 
AAGAACTTTG ATTCTTTATT GTGGGCCATT GTTACAGTTT TTCAGATCCT GACTCAGGAG 
GACTGGAATA AAGTGCTGTA CAACGGGATG GCCTCCACCT CTCCTGTGGC TGCTCTGTAC 
TTCATCGCCC TCATGACCTT TGGGAATTAT GTCTTGTTTA ACCTCTTAGT TGCTATCTTA 
GTGGAGGGAT TCCAAACAGA GGAAATTACC AAGCGGGACG ATTTGCATGG CCAGCTGAGC 
TGTATTCAGC TGCCTATAGA CTCAGGGGGT GATGCCTCAA AGTCGGACTC TGAAGCCGAT 
ATTTATGCAC GGAGCTTGGA GGATGTTTCG GGACACAAGA AAGATATGTC TGCATCATCC 
GTTGTGCCCA TCAATGGCCA TGTGGATCTG AAGAGCAGCC TGACACCGCC TCTGATCACC 
CACACAGCTG CCACACCAAT GCCCATCCCC AAGATATCAG GAGGAGGAGA TTCAGCACTG 
AGCCAAGAGT CCCGCAGAGG CAGCAGTGTC TCCATGGAGC CTAATGGCCA AGACAAATCT 
CCATCCAGTG CACGGAGCTC CCCTAATGCC CCCTTGAGCT CCGCCAGTAG CTGGAACAGC 
AGACGCTCCA GCTGGAACAG TCTAGGCCGA GCCCCCAGCC TCAAGAGGCA GAAACACCAG 
TCTGGTGAAA GGCGCTCCTT GTTGTCTGGT GATGGCCAGT CCAGTTCTGA TGAGGGGGAT 
GCAGGAGAGA GCATGGGGGG CATGTCTGAG GGTGATGATG CCTCCCTCGC CAGGACGGAC 
TCGCTGGGCC AGAGGCCTCG ACATAGGCGC ATGGAGTCCC TGGAGACGCG CAGCTCATTT 
GATCTCCATC CAGACACCCT GCAGGTGCCG TACCCCCACC GCTCCGGCAG CATACACAGC 
GCACGGCCAC CCAACTTCCT CAGCAACGGC AAGACTTCTC CATCTGCAGC CACCACACAG 
CTCTCTTTGG ATGAACACCA CAGTGAAGAC GACAACGCAG ACGAGGAAGG AAACCTGAGT 
CGCAGGGCTC GTCTGTACAG ATGGCTGGAG CACAAGCAGC CCGAATGGTG TCGGCAGAGG 
GTCACCTGGT CCCTTTACCT CTTTCCTCCA GAGTCACGGT TTCGCGTGAC ATGCAATAAA 
ATCATCACCC ACAAGATGTT TGACCATGTG GTTCTTGTCA TCATCTTTCT CAACTGCATC 
ACCATCGCCA TGGAGAGGCC CAGAATCGAC CCCTCTAGCG CTGAACGCAT CTTCTTGACG 
TTGTCCAACT ACATATTTAC TGCCATCTTT GTGACCGAAA TGACAATAAA GGTGGTTGCT 
TTGGGCTTTT GTTTTGGAGA GAAGACCTAC CTGAAGAGCA GCTGGAACAT TCTGGATGGC 
ATGCTGGTGA TGATCTCTGT GATTGACATC CTCGTGTCCC TGATCTCCAA CAGTGGCACC 
AAGATCCTGG GCATGTTACG TGTGCTGCGG TTACTGAGGA CACTCAGGCC ACTCAGAGTG 
ATCAGCAGAG CTCCTGGACT GAAACTGGTT GTTGAGACAT TGATGTCGTC TCTTAAGCCT 
ATCGGAAACA TTGTGGTCAT CTGCTGTGCC TTCTTCATTA TTTTTGGGAT CTTGGGAGTT 
CAGCTTTTCA AGGGAAAGTT CTTTGTGTGT CATGGGGATG ATACGAGAAA CATCACCAAC 
AAGTCAGATT GCCTCCTGGC AAACTACAAA TGGGTCAGAC ACAAGTACAA CTTTGATAAC 
CTTGGACAGG CCCTCATGTC CCTTTTTGTT CTGGCTTCCA AAGACGGTTG GGTGGATATC 
ATGTACGATG GACTGGATGC TGTCGGTGTG GACCAGCAGC CCGTCATGAA TTACAACCCA 
TGGATGCTGC TCTATTTTAT CTCCTTCTTG CTGATCGTGG CTTTCTTCGT GCTCAACATG 
TTTGTCGGCG TGGTCGTCGA GAACTTTCAC AAGTGCAGGA GAAACCAGGA GGAGGAAGAA 
GCCAAACGGC GAGAAGCGAA GCGCCAGAAA CGACGTGATA AAAAACGAAG GAGTAAGGAG 
AAGGAGCTAG CTGAAGCCCA GAGTAAACCC TTTTACTCCG ATTACTGCCC AACACGACGT 
CTCATCTACA ACATGTGCAA GAGCCAGTAT CTGGACCTGT TCATCACCAT CGTCATCGCC 
CTCAATGTCA TCACCATGTC TATGGAGCAC TACCACCAAC CAAAGGTTTT GAGCGATTCC 
CTAAAGATCT GTAACTACAT CTTCACAGTC ATATTTGTGC TGGAGTCCAT CTTCAAACTT 
GTTGCTTTTG GATTTCGCCG CTTCTTCAAG GACAGATGGA ACCAGTTGGA TCTGGCCATT 
GTTTTGTTGT CTATCATGGG CATAACACTG GAGGAAATCG AGGTCAACGC TTCCCTTCCC 
ATCAATCCTA CCATCATTCG CATCATGAGG GTGTTGCGCA TCACTCGAGT TCTAAAGCTA 
CTGAAGATGG CGGTGGGAAT GCGAGCTCTA CTAGACACTG TGATGCAGGC CCTTCCTCAG 
GTGGGGAATC TGGGTCTTCT CTTCATGTTG CTTTTCTTCA TCTTTGCTGC TCTGGGAGTA 
GAGCTGTTTG GTGACCTGAT ATGTGATGAG CTACACCCCT GTGAAGGTCT TGGCCGTTAT 
GCCACGTTCA AAAATTTTGG CATGGCTTTC CTCCTGCTGT TCCGTGTATC CACCGGGGAC 
AACTGGAATG GCATCATGAA GGATACGCTC AGAGACTGCT CCCAAGACAC CGGTATATGC 
TACAACACGG TGGTGTCCCC CATTTACTTT GTGTCCTTTG TGCTGACAGC TCAGTTTGTG 
CTGGTCAATG TGGTGATCGC TGTGCTCATG AAGCACCTAG AGGAAAGCAA CAAAGAGGCC 
AAGGAGGCTG AAGCAGAGGC CGAGCTGGAG CTGGAGCAGG AGATGGAAGC TTTGGGTAGA 
GATAGAGAAA TGCAGAGAGG GCACATGCCA CGTTTGGGTT CTGGCGATTT GGGTGGGCCA 
GGAGGGTCAC CTTGGATATC AAGAGACCAT CGAGACCTGT CAGGACCACT TTACCCCATA 
GACAGCCCAA CAGCAGACAT ACGCAGAGAT TTTGAAGATC ATTTAAGGAC AGATCCTGAT 
CCACCTCCGA ATCTGGAGCC ACCATTAGAG CGGAGGCCTA TGTTTGACTC AGTTTCCCTG 
GTGATCCAGG GCTCGCTGGA AGGAGAGCTC AGCCTCATGG ACAACCTGTC TGGCTCCATC 
TGCCACTACT ACGCTCTGCC CCCCCTTCCC AACAAACACT GCACTGAGAA ACAGATTCCT 
CTAGCCGAGA TGGAGGCTCT ATCTCTGGCA TCGGAGAAAT CCTGGTCCTT AGCTCTGACG 
GATGACTCTG TCCCAGATGA TTTTAACCCC CTCTTACTAA CCAGTCAGGA GTGCAATACC 
ACAGATCCTT CAGATCCACC AGACACCCGG CAGGCGGATG AACCGACGGA TGAACACTTG 
CTATCCGTGA AGAAGACCTC AGTGGGCCGC ACACATTCTC TACCTAATGA CAGCTACATG 
TTCCTGCCGC TGCAGTCCAA CTCAAACCAC AGCACCCACA CAACCTCACC TCACCTCGCT 
CAGACAGGGT CTACAGCTTC TGTTCAGTCT CAAACAGAAG AAGCCAGCCA GCACTTGACG 
GTGCCCAGCG ATCTGTTCAG ACCCATCAGT CCTCACAGTT TATCAGACTC AGAGAGCATC 
CCTCGAATTC CCCCACCTCG GCGGGCACAC ACTCTCTCAC GCACACTCCG CAGACAGGTG 
GCAGTGAGTG CTGACTCTCA GGAGACGCTT TACACAGAGG GAGGGGAAGC TGAGGGTCCT 
CTGGGGCTCA GGGAACTGTC GGACCCCCTT CCACCTCAGC AGCAGCAGCA CCAGCCTTCC 
CTCTTTCTGG TCCCTGCCAC ACCAGGCGCC TCCCCAAAAC CTTCACGACC TAGCGTCCAC 
ACGCAGCACA ACCCCTACGA CCAGTACAAT GTGTTCTCCA GGAGTTCCCA CTCTCCACCT 
GTACCACCAC CACCACCAGA CTATAAGAAG CAGGAGGATG TTGACTCGGT GGATCAGGAG 
GTATCTCGAA TTATCCGAGC GGGACTCACA GGGAGAAGCG ATGATGTCAA CCGAGAAGGG 
ACAGGAGATC AATGTGCAAA ACGTCAGCTT AAGAAATACC ATAGCGTGGA CACTCAAGGC 
CAGCGAGCTC TGCTGTTACC GCGGCCCTTG TCATGGCTTG ATGACCCACG CCGGCACTCC 
ATCGAAGTAT GCTCGTCTGT AGAAAGCAGT CCGCAGCGCA GTTCCATATC GTCAGGGTTT 
GTATCACGAG CCGACAGCCT CCAGCAACAA TCTTCGCCCC GTGGACGCAA GAAGAAGATG 
AGTCCACCGT GCATTTCTGT AGATCCACCC GAGGATCCGG AACTTCCGGG AGGATTCCAT 
CCTGCTCTGG GGATTGTGCC ACCTCCATTG CCAAGCCGGG ACACTTGCCT GAGGAGGCGC 
GCTCCCTCCA GTGAATCCAA GGACTCGTTT GATTTGGGAG GAGGAGAGGC GCTGCCTCAA 
GAGGGCGTTC CAAACTCCAA GCTATTGACT CTGCCTAGCT TCTCTTTCGA GAAAACAAGC 
TCTGAGCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	cacna1g
Entrez Gene ID	563904
Full Name	calcium channel, voltage-dependent, T type, alpha 1G subunit
General protein information	Preferred Names calcium channel, voltage-dependent, T type, alpha 1G subunit Names voltage-dependent T-type calcium channel subunit alpha-1G
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	CACNA1G	NP_938191.2
Danio rerio (zebrafish)	cacna1g	XP_005156372.1
Bos taurus (cattle)	CACNA1G	NP_001180069.1
Xenopus tropicalis (tropical clawed frog)	cacna1g	XP_004918544.1
Mus musculus (house mouse)	Cacna1g	NP_001106284.1
Rattus norvegicus (Norway rat)	Cacna1g	NP_113789.2
Pan troglodytes (chimpanzee)	CACNA1G	XP_511884.2
Gallus gallus (chicken)	CACNA1G	XP_004946372.1
Canis lupus familiaris (dog)	CACNA1G	XP_005624708.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

cacna1g cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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