smurf2 cDNA ORF clone, Danio rerio(zebrafish)

The following smurf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smurf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa38359 NM_001114426.1
Latest version!
Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
ODa08225 XM_005171584.4
Latest version!
Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
View Old Accession Versions >>
ODa08225 XM_005171584.3 Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa38359
    Clone ID Related Accession (Same CDS sequence) NM_001114426.1
    Accession Version NM_001114426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase SMURF2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC155849.1. On Feb 10, 2008 this sequence version replaced XM_684932.2. ##Evidence-Data-START## Transcript exon combination :: BC155849.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCAGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CGGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GCAGTCGTCC CGGACAGTTG
    AAGGAGCAGG CTCAATCTGT CGTGTCCCCC GGAAATCTCC CCGAGGATCC TGAATGCCTC
    ACGGTGCCCA AGTACAAGAG GGATCTGGTT CAGAAGCTGA AGATTCTGAG ACAGGAGCTG
    TCCCAGCAGC AGCCTCAGGC AGGACACTGC CGCATCGAGG TCTCACGCGA GGAAATCTTT
    GAGGAGTCTT ACCGGCAGGT GATGAAGATG AGGCCGAAGG ACCTGTGGAA GAGGCTGATG
    GTCAAATTCA GAGGAGAGGA AGGTCTTGAC TATGGTGGAG TCGCCAGGGA ATGGTTATAC
    TTGCTGTCAC ACGAGATGTT AAATCCATAC TATGGGCTGT TCCAGTATTC ACGAGATGAC
    ATCTACACGC TACAGATCAA CCCGGACTCG GCTGTCAATC CAGAGCATTT GTCTTATTTT
    CACTTTGTGG GAAGGATTAT GGGAATGGCT GTTTTCCATG GTCACTATAT AGACGGGGGC
    TTCACTCTGC CCTTCTACAA GCAGTTGCTA GGCAAACCTA TCACTCTGGA TGACATGGAG
    TCAGTGGATC CGGACCTGCA CAACAGCCTC GTCTGGATCC TAGATAATGA CATCACCGGG
    GTGTTGGACC ACACGTTCTG CGTTGAACAC AATGCATATG GAGAGATCAT TCAGCATGAG
    CTCAAACCCA ATGGCAAGAG CATTCCAGTC ACGCAGGACA CCAAGAAGGA GTATGTGAGG
    CTGTATGTGA ACTGGCGGTT CCTGAGGGGC ATCGAGGCTC AGTTCCTGGC CCTGCAGAAA
    GGCTTCAACG AAGTCATTCC ACAACACCTG CTCAAAGCGT TTGATGAGAA AGAACTGGAG
    CTCATCGTGT GTGGATTGGG AAAGATCGAC ATCAACGACT GGAAGTCAAA CACCCGTCTG
    AAGCACTGCA CCCCTGACAG TAACATTGTG AAGTGGTTCT GGAGGGCTGT GGAGTCGTAT
    GACGAAGAGA GGAGAGCTCG ACTGCTGCAG TTTGTCACCG GATCCTCCAG AGTTCCGCTG
    CAAGGATTCA AAGCCCTGCA AGGTGCCGCT GGTCCACGTC TCTTCACTAT TCACCAGATC
    GATGCCAGCA CCAACAACCT ACCCAAAGCA CACACTTGCT TTAACCGAAT CGACATTCCT
    CCATACGAGA GCTACGATAA ACTCTACGAC AAGCTGCTGA CAGCCATCGA AGAGACATGT
    GGCTTCGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107898.1
    CDS139..2436
    Translation

    Target ORF information:

    RefSeq Version NM_001114426.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001114426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCAGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CGGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GCAGTCGTCC CGGACAGTTG
    AAGGAGCAGG CTCAATCTGT CGTGTCCCCC GGAAATCTCC CCGAGGATCC TGAATGCCTC
    ACGGTGCCCA AGTACAAGAG GGATCTGGTT CAGAAGCTGA AGATTCTGAG ACAGGAGCTG
    TCCCAGCAGC AGCCTCAGGC AGGACACTGC CGCATCGAGG TCTCACGCGA GGAAATCTTT
    GAGGAGTCTT ACCGGCAGGT GATGAAGATG AGGCCGAAGG ACCTGTGGAA GAGGCTGATG
    GTCAAATTCA GAGGAGAGGA AGGTCTTGAC TATGGTGGAG TCGCCAGGGA ATGGTTATAC
    TTGCTGTCAC ACGAGATGTT AAATCCATAC TATGGGCTGT TCCAGTATTC ACGAGATGAC
    ATCTACACGC TACAGATCAA CCCGGACTCG GCTGTCAATC CAGAGCATTT GTCTTATTTT
    CACTTTGTGG GAAGGATTAT GGGAATGGCT GTTTTCCATG GTCACTATAT AGACGGGGGC
    TTCACTCTGC CCTTCTACAA GCAGTTGCTA GGCAAACCTA TCACTCTGGA TGACATGGAG
    TCAGTGGATC CGGACCTGCA CAACAGCCTC GTCTGGATCC TAGATAATGA CATCACCGGG
    GTGTTGGACC ACACGTTCTG CGTTGAACAC AATGCATATG GAGAGATCAT TCAGCATGAG
    CTCAAACCCA ATGGCAAGAG CATTCCAGTC ACGCAGGACA CCAAGAAGGA GTATGTGAGG
    CTGTATGTGA ACTGGCGGTT CCTGAGGGGC ATCGAGGCTC AGTTCCTGGC CCTGCAGAAA
    GGCTTCAACG AAGTCATTCC ACAACACCTG CTCAAAGCGT TTGATGAGAA AGAACTGGAG
    CTCATCGTGT GTGGATTGGG AAAGATCGAC ATCAACGACT GGAAGTCAAA CACCCGTCTG
    AAGCACTGCA CCCCTGACAG TAACATTGTG AAGTGGTTCT GGAGGGCTGT GGAGTCGTAT
    GACGAAGAGA GGAGAGCTCG ACTGCTGCAG TTTGTCACCG GATCCTCCAG AGTTCCGCTG
    CAAGGATTCA AAGCCCTGCA AGGTGCCGCT GGTCCACGTC TCTTCACTAT TCACCAGATC
    GATGCCAGCA CCAACAACCT ACCCAAAGCA CACACTTGCT TTAACCGAAT CGACATTCCT
    CCATACGAGA GCTACGATAA ACTCTACGAC AAGCTGCTGA CAGCCATCGA AGAGACATGT
    GGCTTCGCTG TGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa08225
    Clone ID Related Accession (Same CDS sequence) XM_005171584.3 , XM_005171584.4
    Accession Version XM_005171584.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase SMURF2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171584.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCTGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CAGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GTCGTCCCGG ACAGTTGAAG
    GAGCAGGCTC AATCTGTCGT GTCCCCCGGA AATCTCCCCG AGGATCCTGA ATGCCTCACG
    GTGCCCAAGT ACAAGAGGGA TCTGGTTCAG AAGCTGAAGA TTCTGAGACA GGAGCTGTCC
    CAGCAGCAGC CTCAGGCAGG ACACTGCCGC ATCGAGGTCT CACGCGAGGA AATCTTTGAG
    GAGTCTTACC GGCAGGTGAT GAAGATGAGG CCGAAGGACC TGTGGAAGAG GCTGATGGTC
    AAATTCAGAG GAGAGGAAGG TCTTGACTAT GGTGGAGTCG CCAGGGAATG GTTATACTTG
    CTGTCACACG AGATGTTAAA TCCATACTAT GGGCTGTTCC AGTATTCACG AGATGACATC
    TACACGCTAC AGATCAACCC GGACTCGGCT GTCAATCCAG AGCATTTGTC TTATTTCCAC
    TTTGTGGGGA GGATTATGGG AATGGCTGTT TTCCATGGTC ACTATATAGA CGGGGGCTTC
    ACTCTGCCCT TCTACAAGCA GTTGCTAGGC AAACCTATCA CTCTGGATGA CATGGAGTCA
    GTGGATCCGG ACTTGCACAA CAGCCTCGTC TGGATCCTAG ATAATGACAT CACCGGGGTG
    TTGGACCACA CGTTCTGCGT TGAACACAAT GCATATGGAG AGATCATTCA GCATGAGCTC
    AAACCCAATG GCAAGAGCAT TCCAGTCACG CAGGACACCA AGAAGGAGTA TGTGAGGCTG
    TATGTGAACT GGCGGTTCCT GAGGGGCATC GAGGCTCAGT TCCTGGCCCT GCAGAAAGGC
    TTCAACGAAG TCATTCCACA ACACCTGCTC AAAGCGTTTG ATGAGAAAGA ACTGGAGCTC
    ATCGTGTGTG GATTGGGAAA GATCGACATC AACGACTGGA AGTCAAACAC CCGTCTGAAG
    CACTGCACCC CTGACAGTAA CATTGTGAAG TGGTTCTGGA GGGCTGTGGA GTCGTATGAC
    GAAGAGAGGA GAGCTCGACT GCTGCAGTTT GTCACCGGAT CCTCCAGAGT TCCGCTGCAA
    GGATTCAAAG CCCTGCAAGG TGCCGCTGGT CCACGTCTCT TCACTATTCA CCAGATCGAT
    GCCAGCACCA ACAACCTACC CAAAGCACAC ACTTGCTTTA ACCGAATCGA CATTCCTCCA
    TACGAAAGCT ACGATAAACT CTACGACAAG CTGCTGACAG CCATCGAAGA GACATGCGGC
    TTCGCTGTGG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171641.1
    CDS421..2715
    Translation

    Target ORF information:

    RefSeq Version XM_005171584.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171584.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCTGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CAGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GTCGTCCCGG ACAGTTGAAG
    GAGCAGGCTC AATCTGTCGT GTCCCCCGGA AATCTCCCCG AGGATCCTGA ATGCCTCACG
    GTGCCCAAGT ACAAGAGGGA TCTGGTTCAG AAGCTGAAGA TTCTGAGACA GGAGCTGTCC
    CAGCAGCAGC CTCAGGCAGG ACACTGCCGC ATCGAGGTCT CACGCGAGGA AATCTTTGAG
    GAGTCTTACC GGCAGGTGAT GAAGATGAGG CCGAAGGACC TGTGGAAGAG GCTGATGGTC
    AAATTCAGAG GAGAGGAAGG TCTTGACTAT GGTGGAGTCG CCAGGGAATG GTTATACTTG
    CTGTCACACG AGATGTTAAA TCCATACTAT GGGCTGTTCC AGTATTCACG AGATGACATC
    TACACGCTAC AGATCAACCC GGACTCGGCT GTCAATCCAG AGCATTTGTC TTATTTCCAC
    TTTGTGGGGA GGATTATGGG AATGGCTGTT TTCCATGGTC ACTATATAGA CGGGGGCTTC
    ACTCTGCCCT TCTACAAGCA GTTGCTAGGC AAACCTATCA CTCTGGATGA CATGGAGTCA
    GTGGATCCGG ACTTGCACAA CAGCCTCGTC TGGATCCTAG ATAATGACAT CACCGGGGTG
    TTGGACCACA CGTTCTGCGT TGAACACAAT GCATATGGAG AGATCATTCA GCATGAGCTC
    AAACCCAATG GCAAGAGCAT TCCAGTCACG CAGGACACCA AGAAGGAGTA TGTGAGGCTG
    TATGTGAACT GGCGGTTCCT GAGGGGCATC GAGGCTCAGT TCCTGGCCCT GCAGAAAGGC
    TTCAACGAAG TCATTCCACA ACACCTGCTC AAAGCGTTTG ATGAGAAAGA ACTGGAGCTC
    ATCGTGTGTG GATTGGGAAA GATCGACATC AACGACTGGA AGTCAAACAC CCGTCTGAAG
    CACTGCACCC CTGACAGTAA CATTGTGAAG TGGTTCTGGA GGGCTGTGGA GTCGTATGAC
    GAAGAGAGGA GAGCTCGACT GCTGCAGTTT GTCACCGGAT CCTCCAGAGT TCCGCTGCAA
    GGATTCAAAG CCCTGCAAGG TGCCGCTGGT CCACGTCTCT TCACTATTCA CCAGATCGAT
    GCCAGCACCA ACAACCTACC CAAAGCACAC ACTTGCTTTA ACCGAATCGA CATTCCTCCA
    TACGAAAGCT ACGATAAACT CTACGACAAG CTGCTGACAG CCATCGAAGA GACATGCGGC
    TTCGCTGTGG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa08225
    Clone ID Related Accession (Same CDS sequence) XM_005171584.3 , XM_005171584.4
    Accession Version XM_005171584.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase SMURF2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171584.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCTGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CAGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GTCGTCCCGG ACAGTTGAAG
    GAGCAGGCTC AATCTGTCGT GTCCCCCGGA AATCTCCCCG AGGATCCTGA ATGCCTCACG
    GTGCCCAAGT ACAAGAGGGA TCTGGTTCAG AAGCTGAAGA TTCTGAGACA GGAGCTGTCC
    CAGCAGCAGC CTCAGGCAGG ACACTGCCGC ATCGAGGTCT CACGCGAGGA AATCTTTGAG
    GAGTCTTACC GGCAGGTGAT GAAGATGAGG CCGAAGGACC TGTGGAAGAG GCTGATGGTC
    AAATTCAGAG GAGAGGAAGG TCTTGACTAT GGTGGAGTCG CCAGGGAATG GTTATACTTG
    CTGTCACACG AGATGTTAAA TCCATACTAT GGGCTGTTCC AGTATTCACG AGATGACATC
    TACACGCTAC AGATCAACCC GGACTCGGCT GTCAATCCAG AGCATTTGTC TTATTTCCAC
    TTTGTGGGGA GGATTATGGG AATGGCTGTT TTCCATGGTC ACTATATAGA CGGGGGCTTC
    ACTCTGCCCT TCTACAAGCA GTTGCTAGGC AAACCTATCA CTCTGGATGA CATGGAGTCA
    GTGGATCCGG ACTTGCACAA CAGCCTCGTC TGGATCCTAG ATAATGACAT CACCGGGGTG
    TTGGACCACA CGTTCTGCGT TGAACACAAT GCATATGGAG AGATCATTCA GCATGAGCTC
    AAACCCAATG GCAAGAGCAT TCCAGTCACG CAGGACACCA AGAAGGAGTA TGTGAGGCTG
    TATGTGAACT GGCGGTTCCT GAGGGGCATC GAGGCTCAGT TCCTGGCCCT GCAGAAAGGC
    TTCAACGAAG TCATTCCACA ACACCTGCTC AAAGCGTTTG ATGAGAAAGA ACTGGAGCTC
    ATCGTGTGTG GATTGGGAAA GATCGACATC AACGACTGGA AGTCAAACAC CCGTCTGAAG
    CACTGCACCC CTGACAGTAA CATTGTGAAG TGGTTCTGGA GGGCTGTGGA GTCGTATGAC
    GAAGAGAGGA GAGCTCGACT GCTGCAGTTT GTCACCGGAT CCTCCAGAGT TCCGCTGCAA
    GGATTCAAAG CCCTGCAAGG TGCCGCTGGT CCACGTCTCT TCACTATTCA CCAGATCGAT
    GCCAGCACCA ACAACCTACC CAAAGCACAC ACTTGCTTTA ACCGAATCGA CATTCCTCCA
    TACGAAAGCT ACGATAAACT CTACGACAAG CTGCTGACAG CCATCGAAGA GACATGCGGC
    TTCGCTGTGG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005171641.1
    CDS403..2697
    Translation

    Target ORF information:

    RefSeq Version XM_005171584.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SMAD specific E3 ubiquitin protein ligase 2 (smurf2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005171584.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTCTAATC AAGGAGTTAG AAGGAATGGA CCCGTTAAGT TGCGTTTAAC AGTTCTGTGC 
    GCTAAGAACT TGGTGAAGAA AGACTTCTTC CGGCTACCAG ATCCTTTCGC TAAGGTGGTG
    GTGGATGGTT CAGGGCAGTG CCATTCAACA GACACAGTGA GAAATACTCT GGATCCCAAA
    TGGAACCAAC ACTATGACCT ATATATCGGC AAGTCAGACT CCATCACCAT CAGCGTGTGG
    AACCATAAGA AGATCCACAA GAAACAGGGA GCGGGATTCC TGGGATGCGT ACGACTCCTC
    TCCAACTCTA TTAACAGACT TAAGGACACA GGCTATCAGC GTTTGGACCT GAACAAGTTA
    GGGCCCAATG ACAGCGATAC CGTGAGGGGA CAGATAGTAG TTAGTCTTCA GTCTCGGGAC
    AGGATAGGCA GCGGAGGGCC AGTCGTGGAC TGCAGTCGTC TGTTTGACAA TGACCTGCCT
    GATGGCTGGG AGGAGAGGAG AACTGCATCT GGCCGGATCC AGTATCTGAA TCATATCACA
    CGCAGCACCC AGTGGGAGAG ACCTACCAGG CCTGCATCTG AGTACTCAAG CCCTGGCCGG
    CCTCTCAGCT GCCTGGTGGA TGAAAACACA CCCATCATGA CACCCAACGG GGCAGCGGGT
    GTACCTGCAG ACGACCCACG GGTGCAAGAG CGGAGGGTTC GCTCTCAGAG ACACAGAAAC
    TACATGAGCA GAACTCACCT ACACACACCG CCTGACCTGC CAGAGGGATA TGAGCAACGG
    ACCACGCAGC AGGGCCAGGT CTACTTCCTG CACACACAGA CAGGCGTGAG CACATGGCAT
    GACCCCAGAG TGCCCAGGGA TCTGAGTAAT GTAAACTGTG AGGAGTTGGG TCCATTGCCC
    CCGGGCTGGG AGATAAGGAA CACTGCCACA GGACGAGTAT ATTTTGTGGA CCACAACAAC
    CGAACAACAC AGTTCACAGA TCCACGTCTC TCTGCCAACC TGCATCTCGT CTTAAATCCC
    AGTCCGAATG GCTCGCGTGC TGCAGTTGAA GCTCAGAGCA GTCGTCCCGG ACAGTTGAAG
    GAGCAGGCTC AATCTGTCGT GTCCCCCGGA AATCTCCCCG AGGATCCTGA ATGCCTCACG
    GTGCCCAAGT ACAAGAGGGA TCTGGTTCAG AAGCTGAAGA TTCTGAGACA GGAGCTGTCC
    CAGCAGCAGC CTCAGGCAGG ACACTGCCGC ATCGAGGTCT CACGCGAGGA AATCTTTGAG
    GAGTCTTACC GGCAGGTGAT GAAGATGAGG CCGAAGGACC TGTGGAAGAG GCTGATGGTC
    AAATTCAGAG GAGAGGAAGG TCTTGACTAT GGTGGAGTCG CCAGGGAATG GTTATACTTG
    CTGTCACACG AGATGTTAAA TCCATACTAT GGGCTGTTCC AGTATTCACG AGATGACATC
    TACACGCTAC AGATCAACCC GGACTCGGCT GTCAATCCAG AGCATTTGTC TTATTTCCAC
    TTTGTGGGGA GGATTATGGG AATGGCTGTT TTCCATGGTC ACTATATAGA CGGGGGCTTC
    ACTCTGCCCT TCTACAAGCA GTTGCTAGGC AAACCTATCA CTCTGGATGA CATGGAGTCA
    GTGGATCCGG ACTTGCACAA CAGCCTCGTC TGGATCCTAG ATAATGACAT CACCGGGGTG
    TTGGACCACA CGTTCTGCGT TGAACACAAT GCATATGGAG AGATCATTCA GCATGAGCTC
    AAACCCAATG GCAAGAGCAT TCCAGTCACG CAGGACACCA AGAAGGAGTA TGTGAGGCTG
    TATGTGAACT GGCGGTTCCT GAGGGGCATC GAGGCTCAGT TCCTGGCCCT GCAGAAAGGC
    TTCAACGAAG TCATTCCACA ACACCTGCTC AAAGCGTTTG ATGAGAAAGA ACTGGAGCTC
    ATCGTGTGTG GATTGGGAAA GATCGACATC AACGACTGGA AGTCAAACAC CCGTCTGAAG
    CACTGCACCC CTGACAGTAA CATTGTGAAG TGGTTCTGGA GGGCTGTGGA GTCGTATGAC
    GAAGAGAGGA GAGCTCGACT GCTGCAGTTT GTCACCGGAT CCTCCAGAGT TCCGCTGCAA
    GGATTCAAAG CCCTGCAAGG TGCCGCTGGT CCACGTCTCT TCACTATTCA CCAGATCGAT
    GCCAGCACCA ACAACCTACC CAAAGCACAC ACTTGCTTTA ACCGAATCGA CATTCCTCCA
    TACGAAAGCT ACGATAAACT CTACGACAAG CTGCTGACAG CCATCGAAGA GACATGCGGC
    TTCGCTGTGG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.