ikbkb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ikbkb
Entrez Gene ID	563560
Full Name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Synonyms	ikk2
General protein information	Preferred Names inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta Names inhibitor of nuclear factor kappa-B kinase subunit beta Ikkb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478277.1	XP_021333952.1	inhibitor of nuclear factor kappa-B kinase subunit beta isoform X1
NM_001123265.1	NP_001116737.1	inhibitor of nuclear factor kappa-B kinase subunit beta

Pathways from BioSystems

Homologies

	IKBKB	XP_001096913.1
Mus musculus (house mouse)	Ikbkb	NP_001153246.1
Xenopus tropicalis (tropical clawed frog)	ikbkb	NP_001005651.1
Canis lupus familiaris (dog)	IKBKB	XP_005629860.1
Rattus norvegicus (Norway rat)	Ikbkb	NP_445807.2
Danio rerio (zebrafish)	ikbkb	NP_001116737.1
Bos taurus (cattle)	IKBKB	NP_776778.1
Gallus gallus (chicken)	IKBKB	NP_001026568.1
Homo sapiens (human)	IKBKB	NP_001547.1
Pan troglodytes (chimpanzee)	IKBKB	XP_001142461.2

Gene Ontology
Bibliography

Related articles in PubMed

Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio).
Zhang CN, Zhang JL, Huang Y, Ren HT, Guan SH, Zeng QH
Environmental toxicology33(1)104-111(2018 Jan)

Ikk2 regulates cytokinesis during vertebrate development.
Shen H, Shin EM, Lee S, Mathavan S, Koh H, Osato M, Choi H, Tergaonkar V, Korzh V
Scientific reports7(1)8094(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ikbkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ikbkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31053	NM_001123265.1 Latest version!	Danio rerio inhibitor of nuclear factor kappa B kinase subunit beta (ikbkb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa31053	XM_021478277.1 Latest version!	Danio rerio inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (ikbkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ikbkb ( NM_001123265.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116737.1 Danio rerio inhibitor of nuclear factor kappa B kinase subunit beta (ikbkb), mRNA.

Price & Availability↑

CloneID	ODa31053
Clone ID Related Accession (Same CDS sequence)	XM_021478277.1 , NM_001123265.1
Accession Version	NM_001123265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Danio rerio(zebrafish)
Product	inhibitor of nuclear factor kappa-B kinase subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL954172.9. On Apr 11, 2008 this sequence version replaced XM_686926.2. ##Evidence-Data-START## CDS exon combination :: GFIL01013211.1, GDQH01005003.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGTC CGCCCTCCAT GCAGCCACAG ACGTGCGGCC CCTGGGAACT TAAGGAGCGA 
CTGGGAACCG GAGGATTTGG CAACGTCACT CTCTGGCAAA ATAAGGAGAC CAGCATTCAG 
ATCGCCATCA AACAGTGTCG ACAGGAGCTC AGTGTCAAGA ACAAGGGCAG ATGGAGTTTA 
GAGATTCAGA TCATGAAGAG GTTGACTCAT ATGAATGTGG TTGCGGCGAG AGATGTTCCT 
GAAGAGCTCC AGAAACTGGC CACAAATGAC CTGCCATTGC TGGCCATGGA GTTCTGCCAG 
GGAGGAGACC TACGCAAGTA TCTGAACCTG GTTGAGAACT GCTGCGGTAT GAGGGAAGCT 
GCTGTACTGA CCCTCATTCA AGATATATCC TCAGCGCTGA CGTACCTCCA TGGCATGAGA 
ATAATACACA GAGACCTCAA ACCAGAAAAC ATCGTCCTTC AACAGGGAGA AAAAAGGTTG 
GTTCATAAGA TCATAGATCT GGGTTACGCC AAAGAGCTGG ACCAGAGCAG CCTGTGCACA 
TCGTTTGTAG GGACACTGCA GTATTTGGCC CCGGAGCTGT TGGAGCAGCA GAAGTACACT 
GTGGCTGTGG ATTACTGGAG TTTCGGGACA CTGGTTTTTG AGTGCATCAC AGGATTCAGG 
CCTTTTCTAC CAACCTGGCA ACCCGTTCAA TGGCACAGCA AACTCCAGCA GAAACATGTG 
GATGATATTG TGGTTTATGA GGATCTGGGT GGAGAGGTTC GATACTCTAA ACACCTACCA 
AACCCTCATA ACCTCAACAA ACTGTTAGCT GGTGAGCTGG AGCGCTGGCT GCAGATGATG 
CTGAGATGGT CACCTAAAGA GCGAGGAAAA GACTTAAAAC AGCGCAACAA AGAGAGTCAG 
GAGACGCCCA AAGATGCAGA AGAGGAGAGT AAAGAAACCA GTCCAGAGGG TGCCAAAGAT 
GCTCCAAAAG CCCCCTGCTT CTGCTTTGAT GAATTAAGCC ACATTCTCAA TCTAAAGCTC 
GTCCATGTGC TTAACATGAC CTCTGCTGAA ATATTCAACT ACTCAGTGCA GCCGGAGGAC 
GACGTGGCTT CCCTGCAGGA GCGAATCGCA CAAGACACTG GCATTCCTCC AGCCAATCAG 
GAGCTGCTGC TGGAGGCGGG ACTTGCACTT GAGCCGCAAA ACAGCGTCAT GCAGTGTGCC 
ATCGATTATA GTTTGATGGA CAATCGGCGA GCAGATGAGC CACTGGTTTT CCTGTTTGAC 
CGTTCGTGCT GCAGCTACGA GCCTCAGTTT ACGCCGCGCG TCCTCCCTGA GAACATCCGA 
TTCGTTCAAA ATGAGCCCAA ACGTCCTCTG CCCTACAGCT CTCAGAGACG CACGTGGGGT 
CAAGCGTGGC ACACCATACG AGCGCTGAAA GAAGACTGGC AGAGGCTGCA GCAAGGCCAG 
AAAGTAGCCA TCATGAGTCT CCTCAGACAT AACGGCACAC TGTCAAAGCA GAAGAACGAG 
ATGGTGTCCA TGCACCAGAG GTTAAAAGCC ACGCTGGATT TCTTCGGCGC TAGTCTACAC 
GTCGACATCA GCAAATACCA GGAGCAGAGA GCCACAGGAA TAGCTTCTGA AAAACTGCTG 
AGTGTGTGGA GAGAAATGGA GCAAACGGCC CTCGACTGTG GCCAGACAGA AGACGTGTTT 
AAGCTGGAGG AGGAGATGAT GACCCTTCAG ACTGATATTG TGGATCTGCA GAGACAGCCG 
TGGAGGAGTG GAGAGGCTCT AGAGACGCTT GAAGTGAAGG CCATGGAGCT CTTCCGTAAA 
CTCAGAGAGA AACCCAGAGA GCAGCGCAGT ATGGGCGACT GTCAGGAGGT CATCAAACTG 
GTGGTTCAAG CCGTGCAGTT CTACGAGAGG AAGTTACGGG ACTTTTACAC ACACCTGAGT 
AAGACGATGG TGTGCCGTCA GCGCGTTCTG GCTCTGCTGC CGCGTGTTGA AGGGCTGGTG 
AGCGCCATGG CGAGGAGCGA GAAACAACTG CTGCATTTAC AGGAGAAGCG ACAGAAGGAG 
CTCTGGAACC TGCTCAAAGT GGCCTGCAGT AAAGTTCGCA GTCCAGTGAG TGGCAGTCCA 
GACGGAGGGC GAACTCCACC GGCCCCTACA CCCCAGCTGA TGTCCCACAG ACCCTCACAT 
CCCAGCAGCC CCCCATCAGA CGGCCCACAC ACCGAGGAGT CTCTGCATGT GATTGAGGAA 
AGCAGGATAT TTGAGAACCG CCTGCAGAGT TTAGTGCAAG ACACCATTCA GGAGACGGAG 
AGCAGCATGC AGATTCTGCG GGAGTGGGAG TGGCTGAACG GAGGACAGGA TCTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116737.1
CDS	201..2540
Translation	MSRPPSMQPQTCGPWELKERLGTGGFGNVTLWQNKETSIQIAIKQCRQELSVKNKGRWSLEIQIMKRLTHMNVVAARDVPEELQKLATNDLPLLAMEFCQGGDLRKYLNLVENCCGMREAAVLTLIQDISSALTYLHGMRIIHRDLKPENIVLQQGEKRLVHKIIDLGYAKELDQSSLCTSFVGTLQYLAPELLEQQKYTVAVDYWSFGTLVFECITGFRPFLPTWQPVQWHSKLQQKHVDDIVVYEDLGGEVRYSKHLPNPHNLNKLLAGELERWLQMMLRWSPKERGKDLKQRNKESQETPKDAEEESKETSPEGAKDAPKAPCFCFDELSHILNLKLVHVLNMTSAEIFNYSVQPEDDVASLQERIAQDTGIPPANQELLLEAGLALEPQNSVMQCAIDYSLMDNRRADEPLVFLFDRSCCSYEPQFTPRVLPENIRFVQNEPKRPLPYSSQRRTWGQAWHTIRALKEDWQRLQQGQKVAIMSLLRHNGTLSKQKNEMVSMHQRLKATLDFFGASLHVDISKYQEQRATGIASEKLLSVWREMEQTALDCGQTEDVFKLEEEMMTLQTDIVDLQRQPWRSGEALETLEVKAMELFRKLREKPREQRSMGDCQEVIKLVVQAVQFYERKLRDFYTHLSKTMVCRQRVLALLPRVEGLVSAMARSEKQLLHLQEKRQKELWNLLKVACSKVRSPVSGSPDGGRTPPAPTPQLMSHRPSHPSSPPSDGPHTEESLHVIEESRIFENRLQSLVQDTIQETESSMQILREWEWLNGGQDLA

Target ORF information:

RefSeq Version	NM_001123265.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inhibitor of nuclear factor kappa B kinase subunit beta (ikbkb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123265.1

ORF Insert Sequence:

ATGAGCCGTC CGCCCTCCAT GCAGCCACAG ACGTGCGGCC CCTGGGAACT TAAGGAGCGA 
CTGGGAACCG GAGGATTTGG CAACGTCACT CTCTGGCAAA ATAAGGAGAC CAGCATTCAG 
ATCGCCATCA AACAGTGTCG ACAGGAGCTC AGTGTCAAGA ACAAGGGCAG ATGGAGTTTA 
GAGATTCAGA TCATGAAGAG GTTGACTCAT ATGAATGTGG TTGCGGCGAG AGATGTTCCT 
GAAGAGCTCC AGAAACTGGC CACAAATGAC CTGCCATTGC TGGCCATGGA GTTCTGCCAG 
GGAGGAGACC TACGCAAGTA TCTGAACCTG GTTGAGAACT GCTGCGGTAT GAGGGAAGCT 
GCTGTACTGA CCCTCATTCA AGATATATCC TCAGCGCTGA CGTACCTCCA TGGCATGAGA 
ATAATACACA GAGACCTCAA ACCAGAAAAC ATCGTCCTTC AACAGGGAGA AAAAAGGTTG 
GTTCATAAGA TCATAGATCT GGGTTACGCC AAAGAGCTGG ACCAGAGCAG CCTGTGCACA 
TCGTTTGTAG GGACACTGCA GTATTTGGCC CCGGAGCTGT TGGAGCAGCA GAAGTACACT 
GTGGCTGTGG ATTACTGGAG TTTCGGGACA CTGGTTTTTG AGTGCATCAC AGGATTCAGG 
CCTTTTCTAC CAACCTGGCA ACCCGTTCAA TGGCACAGCA AACTCCAGCA GAAACATGTG 
GATGATATTG TGGTTTATGA GGATCTGGGT GGAGAGGTTC GATACTCTAA ACACCTACCA 
AACCCTCATA ACCTCAACAA ACTGTTAGCT GGTGAGCTGG AGCGCTGGCT GCAGATGATG 
CTGAGATGGT CACCTAAAGA GCGAGGAAAA GACTTAAAAC AGCGCAACAA AGAGAGTCAG 
GAGACGCCCA AAGATGCAGA AGAGGAGAGT AAAGAAACCA GTCCAGAGGG TGCCAAAGAT 
GCTCCAAAAG CCCCCTGCTT CTGCTTTGAT GAATTAAGCC ACATTCTCAA TCTAAAGCTC 
GTCCATGTGC TTAACATGAC CTCTGCTGAA ATATTCAACT ACTCAGTGCA GCCGGAGGAC 
GACGTGGCTT CCCTGCAGGA GCGAATCGCA CAAGACACTG GCATTCCTCC AGCCAATCAG 
GAGCTGCTGC TGGAGGCGGG ACTTGCACTT GAGCCGCAAA ACAGCGTCAT GCAGTGTGCC 
ATCGATTATA GTTTGATGGA CAATCGGCGA GCAGATGAGC CACTGGTTTT CCTGTTTGAC 
CGTTCGTGCT GCAGCTACGA GCCTCAGTTT ACGCCGCGCG TCCTCCCTGA GAACATCCGA 
TTCGTTCAAA ATGAGCCCAA ACGTCCTCTG CCCTACAGCT CTCAGAGACG CACGTGGGGT 
CAAGCGTGGC ACACCATACG AGCGCTGAAA GAAGACTGGC AGAGGCTGCA GCAAGGCCAG 
AAAGTAGCCA TCATGAGTCT CCTCAGACAT AACGGCACAC TGTCAAAGCA GAAGAACGAG 
ATGGTGTCCA TGCACCAGAG GTTAAAAGCC ACGCTGGATT TCTTCGGCGC TAGTCTACAC 
GTCGACATCA GCAAATACCA GGAGCAGAGA GCCACAGGAA TAGCTTCTGA AAAACTGCTG 
AGTGTGTGGA GAGAAATGGA GCAAACGGCC CTCGACTGTG GCCAGACAGA AGACGTGTTT 
AAGCTGGAGG AGGAGATGAT GACCCTTCAG ACTGATATTG TGGATCTGCA GAGACAGCCG 
TGGAGGAGTG GAGAGGCTCT AGAGACGCTT GAAGTGAAGG CCATGGAGCT CTTCCGTAAA 
CTCAGAGAGA AACCCAGAGA GCAGCGCAGT ATGGGCGACT GTCAGGAGGT CATCAAACTG 
GTGGTTCAAG CCGTGCAGTT CTACGAGAGG AAGTTACGGG ACTTTTACAC ACACCTGAGT 
AAGACGATGG TGTGCCGTCA GCGCGTTCTG GCTCTGCTGC CGCGTGTTGA AGGGCTGGTG 
AGCGCCATGG CGAGGAGCGA GAAACAACTG CTGCATTTAC AGGAGAAGCG ACAGAAGGAG 
CTCTGGAACC TGCTCAAAGT GGCCTGCAGT AAAGTTCGCA GTCCAGTGAG TGGCAGTCCA 
GACGGAGGGC GAACTCCACC GGCCCCTACA CCCCAGCTGA TGTCCCACAG ACCCTCACAT 
CCCAGCAGCC CCCCATCAGA CGGCCCACAC ACCGAGGAGT CTCTGCATGT GATTGAGGAA 
AGCAGGATAT TTGAGAACCG CCTGCAGAGT TTAGTGCAAG ACACCATTCA GGAGACGGAG 
AGCAGCATGC AGATTCTGCG GGAGTGGGAG TGGCTGAACG GAGGACAGGA TCTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ikbkb ( XM_021478277.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333952.1 Danio rerio inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (ikbkb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa31053
Clone ID Related Accession (Same CDS sequence)	XM_021478277.1 , NM_001123265.1
Accession Version	XM_021478277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inhibitor of nuclear factor kappa-B kinase subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGTC CGCCCTCCAT GCAGCCACAG ACGTGCGGCC CCTGGGAACT TAAGGAGCGA 
CTGGGAACCG GAGGATTTGG CAACGTCACT CTCTGGCAAA ATAAGGAGAC CAGCATTCAG 
ATCGCCATCA AACAGTGTCG ACAGGAGCTC AGTGTCAAGA ACAAGGGCAG ATGGAGTTTA 
GAGATTCAGA TCATGAAGAG GTTGACTCAT ATGAATGTGG TTGCGGCGAG AGATGTTCCT 
GAAGAGCTCC AGAAACTGGC CACAAATGAC CTGCCATTGC TGGCCATGGA GTTCTGCCAG 
GGAGGAGACC TACGCAAGTA TCTGAACCTG GTTGAGAACT GCTGCGGTAT GAGGGAAGCT 
GCTGTACTGA CCCTCATTCA AGATATATCC TCAGCGCTGA CGTACCTCCA TGGCATGAGA 
ATAATACACA GAGACCTCAA ACCAGAAAAC ATCGTCCTTC AACAGGGAGA AAAAAGGTTG 
GTTCATAAGA TCATAGATCT GGGTTACGCC AAAGAGCTGG ACCAGAGCAG CCTGTGCACA 
TCGTTTGTAG GGACACTGCA GTATTTGGCC CCGGAGCTGT TGGAGCAGCA GAAGTACACT 
GTGGCTGTGG ATTACTGGAG TTTCGGGACA CTGGTTTTTG AGTGCATCAC AGGATTCAGG 
CCTTTTCTAC CAACCTGGCA ACCCGTTCAA TGGCACAGCA AACTCCAGCA GAAACATGTG 
GATGATATTG TGGTTTATGA GGATCTGGGT GGAGAGGTTC GATACTCTAA ACACCTACCA 
AACCCTCATA ACCTCAACAA ACTGTTAGCT GGTGAGCTGG AGCGCTGGCT GCAGATGATG 
CTGAGATGGT CACCTAAAGA GCGAGGAAAA GACTTAAAAC AGCGCAACAA AGAGAGTCAG 
GAGACGCCCA AAGATGCAGA AGAGGAGAGT AAAGAAACCA GTCCAGAGGG TGCCAAAGAT 
GCTCCAAAAG CCCCCTGCTT CTGCTTTGAT GAATTAAGCC ACATTCTCAA TCTAAAGCTC 
GTCCATGTGC TTAACATGAC CTCTGCTGAA ATATTCAACT ACTCAGTGCA GCCGGAGGAC 
GACGTGGCTT CCCTGCAGGA GCGAATCGCA CAAGACACTG GCATTCCTCC AGCCAATCAG 
GAGCTGCTGC TGGAGGCGGG ACTTGCACTT GAGCCGCAAA ACAGCGTCAT GCAGTGTGCC 
ATCGATTATA GTTTGATGGA CAATCGGCGA GCAGATGAGC CACTGGTTTT CCTGTTTGAC 
CGTTCGTGCT GCAGCTACGA GCCTCAGTTT ACGCCGCGCG TCCTCCCTGA GAACATCCGA 
TTCGTTCAAA ATGAGCCCAA ACGTCCTCTG CCCTACAGCT CTCAGAGACG CACGTGGGGT 
CAAGCGTGGC ACACCATACG AGCGCTGAAA GAAGACTGGC AGAGGCTGCA GCAAGGCCAG 
AAAGTAGCCA TCATGAGTCT CCTCAGACAT AACGGCACAC TGTCAAAGCA GAAGAACGAG 
ATGGTGTCCA TGCACCAGAG GTTAAAAGCC ACGCTGGATT TCTTCGGCGC TAGTCTACAC 
GTCGACATCA GCAAATACCA GGAGCAGAGA GCCACAGGAA TAGCTTCTGA AAAACTGCTG 
AGTGTGTGGA GAGAAATGGA GCAAACGGCC CTCGACTGTG GCCAGACAGA AGACGTGTTT 
AAGCTGGAGG AGGAGATGAT GACCCTTCAG ACTGATATTG TGGATCTGCA GAGACAGCCG 
TGGAGGAGTG GAGAGGCTCT AGAGACGCTT GAAGTGAAGG CCATGGAGCT CTTCCGTAAA 
CTCAGAGAGA AACCCAGAGA GCAGCGCAGT ATGGGCGACT GTCAGGAGGT CATCAAACTG 
GTGGTTCAAG CCGTGCAGTT CTACGAGAGG AAGTTACGGG ACTTTTACAC ACACCTGAGT 
AAGACGATGG TGTGCCGTCA GCGCGTTCTG GCTCTGCTGC CGCGTGTTGA AGGGCTGGTG 
AGCGCCATGG CGAGGAGCGA GAAACAACTG CTGCATTTAC AGGAGAAGCG ACAGAAGGAG 
CTCTGGAACC TGCTCAAAGT GGCCTGCAGT AAAGTTCGCA GTCCAGTGAG TGGCAGTCCA 
GACGGAGGGC GAACTCCACC GGCCCCTACA CCCCAGCTGA TGTCCCACAG ACCCTCACAT 
CCCAGCAGCC CCCCATCAGA CGGCCCACAC ACCGAGGAGT CTCTGCATGT GATTGAGGAA 
AGCAGGATAT TTGAGAACCG CCTGCAGAGT TTAGTGCAAG ACACCATTCA GGAGACGGAG 
AGCAGCATGC AGATTCTGCG GGAGTGGGAG TGGCTGAACG GAGGACAGGA TCTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333952.1
CDS	217..2556
Translation	MSRPPSMQPQTCGPWELKERLGTGGFGNVTLWQNKETSIQIAIKQCRQELSVKNKGRWSLEIQIMKRLTHMNVVAARDVPEELQKLATNDLPLLAMEFCQGGDLRKYLNLVENCCGMREAAVLTLIQDISSALTYLHGMRIIHRDLKPENIVLQQGEKRLVHKIIDLGYAKELDQSSLCTSFVGTLQYLAPELLEQQKYTVAVDYWSFGTLVFECITGFRPFLPTWQPVQWHSKLQQKHVDDIVVYEDLGGEVRYSKHLPNPHNLNKLLAGELERWLQMMLRWSPKERGKDLKQRNKESQETPKDAEEESKETSPEGAKDAPKAPCFCFDELSHILNLKLVHVLNMTSAEIFNYSVQPEDDVASLQERIAQDTGIPPANQELLLEAGLALEPQNSVMQCAIDYSLMDNRRADEPLVFLFDRSCCSYEPQFTPRVLPENIRFVQNEPKRPLPYSSQRRTWGQAWHTIRALKEDWQRLQQGQKVAIMSLLRHNGTLSKQKNEMVSMHQRLKATLDFFGASLHVDISKYQEQRATGIASEKLLSVWREMEQTALDCGQTEDVFKLEEEMMTLQTDIVDLQRQPWRSGEALETLEVKAMELFRKLREKPREQRSMGDCQEVIKLVVQAVQFYERKLRDFYTHLSKTMVCRQRVLALLPRVEGLVSAMARSEKQLLHLQEKRQKELWNLLKVACSKVRSPVSGSPDGGRTPPAPTPQLMSHRPSHPSSPPSDGPHTEESLHVIEESRIFENRLQSLVQDTIQETESSMQILREWEWLNGGQDLA

Target ORF information:

RefSeq Version	XM_021478277.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta (ikbkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478277.1

ORF Insert Sequence:

ATGAGCCGTC CGCCCTCCAT GCAGCCACAG ACGTGCGGCC CCTGGGAACT TAAGGAGCGA 
CTGGGAACCG GAGGATTTGG CAACGTCACT CTCTGGCAAA ATAAGGAGAC CAGCATTCAG 
ATCGCCATCA AACAGTGTCG ACAGGAGCTC AGTGTCAAGA ACAAGGGCAG ATGGAGTTTA 
GAGATTCAGA TCATGAAGAG GTTGACTCAT ATGAATGTGG TTGCGGCGAG AGATGTTCCT 
GAAGAGCTCC AGAAACTGGC CACAAATGAC CTGCCATTGC TGGCCATGGA GTTCTGCCAG 
GGAGGAGACC TACGCAAGTA TCTGAACCTG GTTGAGAACT GCTGCGGTAT GAGGGAAGCT 
GCTGTACTGA CCCTCATTCA AGATATATCC TCAGCGCTGA CGTACCTCCA TGGCATGAGA 
ATAATACACA GAGACCTCAA ACCAGAAAAC ATCGTCCTTC AACAGGGAGA AAAAAGGTTG 
GTTCATAAGA TCATAGATCT GGGTTACGCC AAAGAGCTGG ACCAGAGCAG CCTGTGCACA 
TCGTTTGTAG GGACACTGCA GTATTTGGCC CCGGAGCTGT TGGAGCAGCA GAAGTACACT 
GTGGCTGTGG ATTACTGGAG TTTCGGGACA CTGGTTTTTG AGTGCATCAC AGGATTCAGG 
CCTTTTCTAC CAACCTGGCA ACCCGTTCAA TGGCACAGCA AACTCCAGCA GAAACATGTG 
GATGATATTG TGGTTTATGA GGATCTGGGT GGAGAGGTTC GATACTCTAA ACACCTACCA 
AACCCTCATA ACCTCAACAA ACTGTTAGCT GGTGAGCTGG AGCGCTGGCT GCAGATGATG 
CTGAGATGGT CACCTAAAGA GCGAGGAAAA GACTTAAAAC AGCGCAACAA AGAGAGTCAG 
GAGACGCCCA AAGATGCAGA AGAGGAGAGT AAAGAAACCA GTCCAGAGGG TGCCAAAGAT 
GCTCCAAAAG CCCCCTGCTT CTGCTTTGAT GAATTAAGCC ACATTCTCAA TCTAAAGCTC 
GTCCATGTGC TTAACATGAC CTCTGCTGAA ATATTCAACT ACTCAGTGCA GCCGGAGGAC 
GACGTGGCTT CCCTGCAGGA GCGAATCGCA CAAGACACTG GCATTCCTCC AGCCAATCAG 
GAGCTGCTGC TGGAGGCGGG ACTTGCACTT GAGCCGCAAA ACAGCGTCAT GCAGTGTGCC 
ATCGATTATA GTTTGATGGA CAATCGGCGA GCAGATGAGC CACTGGTTTT CCTGTTTGAC 
CGTTCGTGCT GCAGCTACGA GCCTCAGTTT ACGCCGCGCG TCCTCCCTGA GAACATCCGA 
TTCGTTCAAA ATGAGCCCAA ACGTCCTCTG CCCTACAGCT CTCAGAGACG CACGTGGGGT 
CAAGCGTGGC ACACCATACG AGCGCTGAAA GAAGACTGGC AGAGGCTGCA GCAAGGCCAG 
AAAGTAGCCA TCATGAGTCT CCTCAGACAT AACGGCACAC TGTCAAAGCA GAAGAACGAG 
ATGGTGTCCA TGCACCAGAG GTTAAAAGCC ACGCTGGATT TCTTCGGCGC TAGTCTACAC 
GTCGACATCA GCAAATACCA GGAGCAGAGA GCCACAGGAA TAGCTTCTGA AAAACTGCTG 
AGTGTGTGGA GAGAAATGGA GCAAACGGCC CTCGACTGTG GCCAGACAGA AGACGTGTTT 
AAGCTGGAGG AGGAGATGAT GACCCTTCAG ACTGATATTG TGGATCTGCA GAGACAGCCG 
TGGAGGAGTG GAGAGGCTCT AGAGACGCTT GAAGTGAAGG CCATGGAGCT CTTCCGTAAA 
CTCAGAGAGA AACCCAGAGA GCAGCGCAGT ATGGGCGACT GTCAGGAGGT CATCAAACTG 
GTGGTTCAAG CCGTGCAGTT CTACGAGAGG AAGTTACGGG ACTTTTACAC ACACCTGAGT 
AAGACGATGG TGTGCCGTCA GCGCGTTCTG GCTCTGCTGC CGCGTGTTGA AGGGCTGGTG 
AGCGCCATGG CGAGGAGCGA GAAACAACTG CTGCATTTAC AGGAGAAGCG ACAGAAGGAG 
CTCTGGAACC TGCTCAAAGT GGCCTGCAGT AAAGTTCGCA GTCCAGTGAG TGGCAGTCCA 
GACGGAGGGC GAACTCCACC GGCCCCTACA CCCCAGCTGA TGTCCCACAG ACCCTCACAT 
CCCAGCAGCC CCCCATCAGA CGGCCCACAC ACCGAGGAGT CTCTGCATGT GATTGAGGAA 
AGCAGGATAT TTGAGAACCG CCTGCAGAGT TTAGTGCAAG ACACCATTCA GGAGACGGAG 
AGCAGCATGC AGATTCTGCG GGAGTGGGAG TGGCTGAACG GAGGACAGGA TCTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dibutyltin depressed immune functions via NF-?B, and JAK/STAT signaling pathways in zebrafish (Danio rerio).
Environmental toxicology33(1)104-111(2018 Jan)
Zhang CN,Zhang JL,Huang Y,Ren HT,Guan SH,Zeng QH

Ikk2 regulates cytokinesis during vertebrate development.
Scientific reports7(1)8094(2017 Aug)
Shen H,Shin EM,Lee S,Mathavan S,Koh H,Osato M,Choi H,Tergaonkar V,Korzh V

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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ikbkb cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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