gRNAs
The following fras1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fras1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa01500 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001130840.1 | |
| Accession Version | NM_001130840.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11913bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-07 | |
| Organism | Danio rerio(zebrafish) | |
| Product | extracellular matrix protein FRAS1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571800.8, CR392333.5 and BX640536.6. On Aug 5, 2008 this sequence version replaced XM_001921096.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. | |
1 | ATGCCCTCAG ACACTGAGGA ACGCCCACCC CGGGAGACAC AGAGCCCCCC ACCAGCATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001124312.1 |
| CDS | 1..11913 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001130840.1 |
1 | ATGCCCTCAG ACACTGAGGA ACGCCCACCC CGGGAGACAC AGAGCCCCCC ACCAGCATAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa10722 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005165335.3 , XM_005165335.4 | |
| Accession Version | XM_005165335.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 12012bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | extracellular matrix protein FRAS1 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165335.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005165392.1 |
| CDS | 365..12376 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005165335.3 |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa10722 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005165335.3 , XM_005165335.4 | |
| Accession Version | XM_005165335.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 12012bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | extracellular matrix protein FRAS1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005165392.1 |
| CDS | 93..12104 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005165335.4 |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa142680 | |
| Clone ID Related Accession (Same CDS sequence) | XM_021476261.1 | |
| Accession Version | XM_021476261.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 11979bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | extracellular matrix protein FRAS1 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_021331936.1 |
| CDS | 358..12336 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_021476261.1 |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa44084 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009301656.2 , XM_009301656.3 | |
| Accession Version | XM_009301656.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 12009bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | extracellular matrix protein FRAS1 isoform X2 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009299931.1 |
| CDS | 365..12373 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009301656.2 |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa44084 | |
| Clone ID Related Accession (Same CDS sequence) | XM_009301656.2 , XM_009301656.3 | |
| Accession Version | XM_009301656.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 12009bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | extracellular matrix protein FRAS1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_009299931.1 |
| CDS | 358..12366 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_009301656.3 |
1 | ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Pharyngeal morphogenesis requires fras1-itga8-dependent epithelial-mesenchymal interaction. |
Protein-Trap Insertional Mutagenesis Uncovers New Genes Involved in Zebrafish Skin Development, Including a Neuregulin 2a-Based ErbB Signaling Pathway Required during Median Fin Fold Morphogenesis. |
Canonical Wnt signalling regulates epithelial patterning by modulating levels of laminins in zebrafish appendages. |
Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. |
