fras1 cDNA ORF clone, Danio rerio(zebrafish)

The following fras1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fras1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa01500 NM_001130840.1
Latest version!
Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa10722 XM_005165335.4
Latest version!
Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa142680 XM_021476261.1
Latest version!
Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa44084 XM_009301656.3
Latest version!
Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa44084 XM_009301656.2 Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa10722 XM_005165335.3 Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa01500
    Clone ID Related Accession (Same CDS sequence) NM_001130840.1
    Accession Version NM_001130840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-07
    Organism Danio rerio(zebrafish)
    Product extracellular matrix protein FRAS1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571800.8, CR392333.5 and BX640536.6. On Aug 5, 2008 this sequence version replaced XM_001921096.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    ATGCCCTCAG ACACTGAGGA ACGCCCACCC CGGGAGACAC AGAGCCCCCC ACCAGCATAC 
    GTGGTGAGGT GGTCTCTCCC TTTCTCCGCG GACCCTGGTT CAAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGAAAAA CTGGTCCATT TGCCTGGAAA GTGCTGCCCA GAGTAATCTA
    GAGATTGTCA GAGAAGGGTT GTGTCGAGAG TGCCAGTGTC AAGAGGGTCA CGTGACCTGC
    TACCAGCACT CCTGTCCCAC CTGTCCACTG GGAACACTAA CTATACCTCA CCGGGAGCAG
    TGCTGTCCCG ACTGCAATCC AGAAGTAAAG TGCCACGAGG ACTGTCTGAC ATGCTCTGGC
    ACACCGGATC ACTGTGCCAG CTGTCGAGAC CCTTCTGCCC TGCTAATGAA CGGACGCTGT
    CTGCAGGCCT GTCCTCAAGG CTTTTTCGCT CAGGGCAAGG TTTGTGCAGC CTGCCAGCCG
    TCATGTGCCA CCTGTGAGAA TGACTTTGAA TGCACAGCGT GCGGTGGGTC GTTTCTGCTC
    AGTGGCAGGC AGTGTGTGGC CACCTGTGAG AGAGGTCTGT TTCAGGCCCA CACACACTGC
    CTCAGTTGCC ATGACTTATG CTCATCGTGT CAGGGGGCGG GGCCTCAGGA TTGTTTGACA
    TGCTCAGACT CCTCCCACTT ACTGAAGGAT GGATACTGTG TTTCAGACTG CGGCCCAGGC
    TTTTACACAA GTCAAGGCAA CTCTTGTGAC CCTTCCTGTG CAACCTGCCA ACCTGACAGC
    CCCAGCTGTA TGAGCTGCCC TGCAGAACAT GCACTGCATC AAGGGAAATG TGCACCACAA
    TGTCCAGCGC AGTATTACAA GGATGACCAC GGCCGATGCC AAGCTTGCCA CAGTTCGTGT
    GCGTCCTGCT CGGGGCCTGC TGTGTCTCAC TGCACCTCCT GCTCAAAGGC TCTGATCTTG
    AACCAGGGCC AGTGTGTGGA GTCCTGTGGA GAGGGCCTGT TCTCCAGAGC GGGACACTGT
    CACAGTTGTC ATTCGTCCTG TAGGGTCTGT GTGGGTCCAG ACTCCTCAGA CTGCACACAG
    TGTGTCAAAA TGGAAGAGGT GCTGCAGCCT CAAAATGGAC ATGTCTCTCA CGGGTCCTGC
    ATGTCCGCCT GCAGGCCACA GCATTACCTT GAAACAGATC GGACCTGCAG AGAATGCCAC
    AGCTCATGTT CAGGCTGCAT TGGTGGAAGT TTTCAGAACT GTACCTCCTG TTTGCGACCC
    GGTGTCCTTC ATCAAGGGCA GTGCCTCAGC AAATGTCCCA ATGGCTTCTT CCACATTCAG
    GACCTCACTT CTTGTCATCC ATCATGTAAG GAGTGCACTG GCACATCTCA GGCCGACTGT
    TCTGCCTGTC CCGCTCATGC CAGTCTTCAT AACGGTTACT GCCGAACCAG CTGCCCCGAG
    GGCCAGTACC TCAATTCTGT GGGTTTCTGC GATGAGTGCG AGGAGGGATG CCAGCGCTGT
    GTCGCAGACC TGCAGTCTGG CTCTGGCACT GTGTGTTTGT GGTGTAAAGT TCCCAGAATG
    CTTCTGCTGG GCAATCACTG TGTTCCCGAG TGCCCACACA AACACTACCG CTGGCATGGA
    GCCTGCAAAA AATGTCACTC TTCCTGTGAG GAGTGCAGTG CTGATGGTCC TCTTTCTTGT
    ACCTCTTGTT TGGCCCCTGA AGTTTTGGCC CCATCAGGAC TCTGTAGTCC ACGCTGTCCG
    ACTGGATATT ATGCTGATGA AAACCGAGTT TGCCAAAAGT GTGATTCTCA GTGTCTGAGC
    TGTGAGATGG CAGGCGTGTG CTCGTCCTGT AGAGATCCAG CTAAAGTGCT TCTGTTTGGG
    GAGTGTCAGT ATGAGAGCTG TGCTCATCAG TATTACCTCA ACACCAGCAC ACGCACATGC
    AGAGAATGCG ACTGGAGCTG TAATGCGTGC CGTGGACCTC TGCGCAGTGA CTGCCTGCAG
    TGCATGGAGG GATACGTCCT GCAGGATGGC ATTTGTGTGC CGGGTTGCTC ACCGGGATTC
    TACCAGGATG CAGAGAGATG TCTGAGCTGT GAAGAGCACT GTGAGCAGTG CCAGGCTCAT
    GGTCAGTGCC AGCGGTGCCA GTTGCCGTAT GCCTCATTTA ACGGACATTG TGTGCTGGAA
    TGCAGAAAGC ACTATTTTCT AGATGACTCG ACTCGACAGT GCACCGCATG CTCAGCTGGT
    TGTGTGGAGT GTGTGAGTGC AGGCGAGTGT CGCGTCTGTG CTGAAAACAC CTTCCTGAAG
    GATGGCAGAT GCATCCCAGA CTGTGGTCAT GGATATTATG CTGACCTAAA AACAAGAGCT
    TGTCATGCAA ATAGTCGTGC TCCTTCTCTT TACATCAATG GCTCTCTGCT GGTTCCTATT
    GGTGGAGTTA AGCCTCTGGA TCCAGCACTC CTGTCAATTA AGGATGCAGA TAGCCAATCA
    GAGAGCATGT TGCTTCAGCT CCTTCAGCCG CCCAGTAATG GCCACCTCAG GCTGCTGGAG
    AATGACAGAG AGAGATCGCT GGGCAAAGAT GACACTTTCA CCTATACTCA GCTCAAACGC
    AGAGCGGTGC GCTTCATTCA CGACAAAGAG CAGACCAAAA GTGGGCAGTT CACGTTAAGG
    GCAGCAGATC CTCAACTGTT CTCTCAGCCT CAGACTGTTA CTGTGCAGGC TCTCTCTCAG
    CAGCCACCCA CTGTAATCAC CAACCAAATC CTGTACATTA ACTCTGGCGA GACAGCCGTC
    ATCAGCAGAT CTATTCTACA CATCAGCGAC CTTGACAATC CTCAGGACGT GTTCATCACG
    GTTCTGGATC CACCTCAGCA CGGTCGCCTG ACATACATGC ACAGTGATAC GCAGGTCACC
    CATTTTAAAC TGGAAGAGCT AGATGGCGAG CAGCTGCAGT ATGTTCATGA TCGTTCAGAT
    GGAGACCAGG ACAGACTTCT GCTGCAGGTC AATGACGGAC ACAGCTACCA GAACGTCCTG
    TTCCACATCA GCATTGAACA GAAGGTTGGA TTGACTCCGC ACCTGCGTTC AGTGCCCAAA
    ACTTGGGTGA AGGAGGGAGG AATGGTGCAG CTTTCTAAAA AGTGTCTCAA TGCTCAATTC
    AAAGGAGCCA GTGACTCTGA GATCCTGTAC AGCATCCATT CTGAGAGCGG ACAGCCCAAA
    TACGGAGAGA TTGTGCTGGT GTCTATGCCC GCTGACGGTC CTGTGGAAGC GTGGCAAGCC
    TTTTCTGATG GTCAAGACTC TACACCCACC AGCTCATTTA CACAGCAAGA TGTAAATGAA
    GGCACTGTCT GGTACCGACA TTATGGATCT GGAACACAGA GAGACACTTT TCAGTTTCAG
    GTTTCAAGTG AGATCTATCC AGAGACTCTG AGCGATACAC AGACCTTCAC TATAGGAGTG
    ATGCCTCAGA GCCCTGGCAT GCCTCAGCTT TCACCTGGAG TCGATCTGCA GATCACTGCG
    CTGGAAGATC GCGTGACTGT GATCTCTCCA TCTGCTCTCT CCTTCACTGA CTCTGAGACA
    CCCAGCGACA AGCTTGTCTA CAACATCACC AAAGCGCTCA CCTCAGGCCA CGGTGTGCTG
    GAGCACAAGG ACAGACCTTA TCATCATGTG CGTCTCTTCA CCCAGGCTGA CGTAGATTCT
    GGCAAAATTA TCTACAGACC CCCACAGGCC CCATCTCATC TTCAGGAGCT TTATCAATAC
    TCATTTACAG GTCTGCCAGA ATCAGTGAGC GTCCATTTCA CAGTGTCTGG TGGAGAACAC
    ACCACCCCAG AGATGGACTT TGCCATCCTG CTTTTGGTCA ATCATCAGCA GCCACCAGTC
    TTTCAGATCC TGGACCCAGT GCTGGAGGTC AGCCTTGGCG GCAGGGCACC AGTGGGTGTG
    CAACAGTTGG CAGTAACAGA TTCAGACACG GCCCCTGAAG AGCTGGAGTT TGAGGTGGTG
    GAGGGACCAC TACATGGGAC GCTCCTGAAA GTGGACCTTG GATCCCAGGT CCAGATGGTC
    AATGGGGACA CCTTTACCTC CAGTGATGTC ACCAGAAACA TCCTGCAGTA TGAACATGCA
    GGGCTCACCA CTGAAGAGGA CTCCATGACC TTCTCAGTGA CTGACGGGAT CTCTATGACC
    TCCATCGCGG TGCAGGTTTT GGTGTCAGAG GTCAAAGGCG ACGCACCCAA GCGTGACCCA
    AAGGCTTTTC TTTCCATGGA AGTTGCAGAG AAGAGCAGCA GCATCATTAG ACGTTCACAT
    TTAGCTTATA TGGATGATGC ATCTCCAGAT GAGAGGATTT TTGTGCAGTT AGTCTCTGTG
    CCAATGTATG GGATCCTTAC AAAAAATAAA GCTGGAGCAG ACTATGAGGA GCTTAGTGAA
    TATTCTTCCT TTACAATGGA GGACATCAAC AACCACAAAA TAAGGTATGT TACGTCCTTT
    GAAACTGGGA ACCAGCCTGT GACTGACATT TTCCACTTCG TCGTCTATGA TGGTGATAAC
    AATCGTCTGG AAAACCAGAT GTTCACTATC ACTATCACCA CTACCCAGAG GCAGCCACCT
    GTTGTCAGCG TCCGCAGTGG CATTAAAGTT CAGGAAGGTG GGAGAGTCCA GCTCTCAACC
    AATCACATTA TTGCATCTGA CCCAGACACA CCCAGAAAGG ACCTGCTGGT TTGGCTGATC
    AGACCACCTA AATTTGGCTT CATTGAGAAC ACAAAGCAAG CAGTGGTTGG TGAATCTCGC
    GTGATCAGCA CTGATGTCCC CTTCACTATT GAAGACCTCA ATTCAGACCA TATCTTCTAC
    GTGCAAAACA TTCGCCAATC CAATATTCGA CAGGATGTCT TCAGCTTTTA TATCAGTGAT
    GGATCAAGTC AAACTGAGGC TTTTGATGTC ACTATAGATA TTCAGCACAC CAAAGAGGAC
    CTAGTTCCAA TTATCTCTGT AAATAGCATC CACGTGGAGG AGAACTCTGG TGTGGTCATT
    ACCAACTCCT CCCTTAACGT ACTGGACCGG GACACGCCAG AGAATGAGCT TTTGCTTACC
    ATCATCAAGA AGCCTTCATA CGGGAAGATA CGCAGAAGGC AGTTTTACTC AGACCCTTTA
    GAGAATGGCA GGATTCTTCA GCAGGGATCC ACCTTCACCT ACCAGGACAT TCTTGATGAG
    CTGCTGGTCT ACACTCCAGA CGGACGTGGC TCTGGGACTG ATGAGATCCA GTTTTCAGTA
    ACAGACGGCA TATACACGGA GACGGGCCGT CTGGAGTTCA GTATGGATAT TAGGAAGAAA
    GAAGGCCCTA GAGTAACAAT CAACAGAGGA CTGCAGCTCG CAGCAGGTTC TTCATCTAAA
    ATCACGGAGC AGCATCTGAA AGGAACGGAC ATAGATTCAG ATAACCTAAA GCTGAGGTTC
    ACCCTCATCA AAGACCTAAG TGTGGGCTCC CTGATGCTGA AAACAGGCAG AAACAAGGCT
    CGCGTATCCG TGAAAGGACC CGTCACCAGC TTCACCCAGG ATGACATCAA CAAAGGACAT
    GTGGAATACA TTCATGAAAA AGGAGAGAGC GGAGGAAGCT TCTCTTTTAA GTTCAACCTA
    GAAGATCCTG AGGGAAACAG AGTCATTGAC CAGTCTTTCT TCATTAGTGT TCTGGAGGAC
    AGACTTCCTC CCACAGTGGT GGTTAATAAG GGTTTAGTTT TGGATGAGAA CTCCTTGAAG
    AAGATCACAA CTCTGCAGCT CTCCTCTACT GATCAGGATA GTGAACCCGA TGAACTCGTT
    TACCGAATCA CTAAACAGCC CCAGCTTGGT CATCTAGAAC ATTCTAACAA ACCAGGAACA
    CGGATCAGCT CGTTTACCCA GGCTGACCTG GCCTCTCGCA GCATTCAGTA TGTGCACACC
    AGTGAAGACG AGATTCACTC CGATGACTTC ACCTTCACTG TATCTGATGG AGGAAACGAG
    GTCTCACAGA CGTTCTACAT CACCATCCGG CCGGTGGACG ATGCTCTGCC TGTGTTGCAG
    GTGCCCGGCA TGAGGGTGCA GGAGGGCGTG AGGAAGACCA TCACTGAGTT TGAGCTCAAG
    GCCACCGATG CTGACACTGA GGAGGACTCT GTCACTTTCA CCATTGTTCA GGCACCTCGT
    CATGGGACCA TCGAGCGCAC CAGTAATGGT CAGCATTATC GTCAGACAAA CACCTTCACT
    ATGGACGACG TCTACCAGAA CCGCATCAGT TACAACCATG ATGGCAGTAA CTCCCTCAAA
    GATCGATTCA CCTTCACTGT CGGTGACGGC ACCAACATGT TCTTCATCAT CGAGGAGGGT
    GGAAAAGAGG TTGTCACTGC TGCTCCTCAA AAGTTTAAAA TTGACATTTT ACCTGTTGAT
    GACGGGACCC CTCGTATTGT TACCAACCTG GGCCTGCAGT GGCTGGAGTA CATGGAAAAT
    AAGGCCACTA ATCTGATCTC CAAGAAGGAG CTTTTCACTA TCGATCCAGA CACAGATGAT
    GGACAGCTGA CTTATGAAAT CACTACAGAA GCTAAGCACG GCTACCTGGA GAGTAAACTC
    AATCCTGGCA AACCTATCAC CTCTTTCACA CAAGCTGACA TAAACCTTGG CCTTATCCGA
    TACGTTCTAA GCGAGGACAA TGTTCAGGAG ACCATGGACA ACTTCAAGTT CCTTGTAAAG
    GACAGCAAAC CTAACATCGT CAGTGACAAT GTGTTCCACA TCCAGTGGTC TCTGATCAGC
    TTTGAACACA AGAGTTTTAA CGTGAGTGAG AAGGATGGAA CGGTGGCGGT CACAGTGAAG
    AGGACTGGTA ATCTGAACCA GTATGCTATT GTGCTGTGTC GAACTGAGCA GGGCACTGCC
    ACCTCCACCT CCAGCATGGG CTCTCGGGCC GGACAGCAGG ATTATGTTGA ATACGCCGGA
    CAGGTTCAGT TTGATGAGAG AGAAGACACC AAGGTGTGCA CCATTGTGAT CAACGATGAC
    CAGGTGTTTG AGAACATCGA GAGCTTTAAT GTGGAGCTGA GTATGCCGGT GTATGCCCTC
    CTGGGCTCAG TGACCCGAGC TAAAGTCAAT ATCAATGACA CGGAGGATGA GCCCACTTTG
    CAGTTTGATA AGAAAACCTA CCACGTCAAT GAGAGCACTG GTTTCATATT TGCTCCTGTT
    GAGAGAAAAG GTGACACCAG CAGCACAGTT TCTGCCCTGT GCTACACTGT GCCTAAATCA
    GCTCGGGGCA GCAGTCTTCA CGCTCTCGAG TCCGGCTCAG ACTACAAGAC CCGTGGCATG
    AGTGGCGACA ACAGACTCAT CTTTGGTCCA GGAGTGAGCA TGTCCACCTG TGACGTCAAG
    CTGATCGATG ACAGCGAGTA TGAACTCTCT GAGGAGTTTG AGCTGGTGCT GTCAGACGCC
    TCAGATAATG CAAGGATGGG CGACGTCACT GTGGCCAAAG TCATCATCGA CGGGCCCAAC
    GACGCCTCTA CTGTGTTTCT GGGAAACGCA TCATACACCT TCAGTGAAGA TGCAGGCACC
    ATTGAAATCC CTGTTCTGCG GCAGGGCAGT GATCTTTCAT CAGTCACCTC AGTGTGGTGC
    GCCACACGTC CTGCAGAACA AGATTCAGCC ACGCCTGGAG TCGACTACAT CCCCAGCTCC
    AAAAAAGTAG AGTTCAAACC AGGCAAGACC GAGGAGACAT GCAGTTTGAC CATAATGGAT
    GACATCCAGA ACCCAACAAT AGAAGGGAAA GAATCCTTCA TTGTGTTTCT TAGCTCTGCT
    AATGGAGCGG TCCTGACTGA ACCCTATGAA GCCTCTGTCT ACATCACTGA CACCACTCAA
    GACATCCCAA GCATGCAGTT TGACAAGCTG GCATATACAG TAAAGGAAAA AGATGGCGTC
    CTGCACATCC CTATTTTTAG GACAGGAGAT CTGTCCTATA AGTCTTCTGT CCGCTGTTTC
    ACCCGCACAA TGTCTGCAAT GGTTATGGAT GACTTTGAGG AGAGGAGAAA TGCAGATGAT
    TCGAGAATCA CCTTTCAGAA AGGGGAGAAG GTGAAGAATT GTACAGTACT GATCCAGGAT
    GACTCCGTGT TTGAGCCTGA AGAGGAGTTC CAGGTCCACC TTGGAAGCCC ACAGGGTGAC
    CATTGGATTG GAGCCATGAT TGGAGTCAAC GACATTGTGA CTGTGACCAT CACAAATGAT
    GAAGATGCAC CTACGATCGA GTTTGAACAA GCCACATATC AAGTTCGTGA GCCACCTGGT
    CCCGATGGCA TTGAGGTTTT GAATATCAAG GTAATTCGTA AAGGAGACCT CGACCGCACC
    TCCAAAATTC GATGCAGCAC GAGGGATGGA TCGGCCCAGT CAGGTGTGGA CTATAACCCA
    AAGAGTCGAG TGCTGAAGTA CAGCCCTGGT GTGGACCACA TCATGTTTAA GGTGGAGATT
    CTGTCCAATG AGGACAGAGA GTGGCATGAG TCTTTCTCTT TGGTCCTTGG TCCTGACGAC
    CCAGTTGAAG CTGTTCTTGG AGAGATCATC ATTGCAACAG TCACTATTCT GGATCAGGAG
    GCTGCAGGAA GTCTTATCCT TCCGGCTCCA CCCATTGTGG TGTCTCTATC AGATTATGAC
    TACGTTCAGG AGGTCACTAA AGAGGGCAGC AAGAAGTCTC CGTCTCCTGG ATATCCGCTG
    GTCTGTGTGA CCCCCTGTGA CCCCCACTAC CCCAAATACT CCGTCATGAA GGAGCGCTGT
    GAGGAGGCAG GCATCAACCA GACTTCTCTA CACTTCAGCT GGGAGGTGGC AACTCCTACA
    GACACCAGCG GAGCAAGATC TCCCTTCGAG ACTGTGACCG ACAACACACC CTACACCACA
    ATCAACCACA TGGTCCTGGA CAGCATTTAT TTCAGCAGGC GCTTCCATGT GCGCTGTGTA
    GCCCAAGCCA GAGATAAAGC CGGACACCTG GGAACTCCAC TGAGGAGCAA CATTGTCACC
    ATTGGCACTG AGGGATCAAT ATGCCACACT CCAGTCACCA CAGGGACCGC TCGAGGCTTC
    CAAGCCCAGT CCTTTATCGC CACTCTAAAA TACCTGGATG TCAAACACAA AGAGCATCCA
    AACAGGATCC ACATTTCAGT TCAGATTCCC CATCAGGATG GGATGCTTCC ACTGGTGTCC
    ACCATGCCTC TGCATAACCT GCACTTCCTG CTCTCTGAGT CCATTTACCG TCAGCAACAC
    GTCTGCTCCA ACCTGGTCAC ACTTAAAGAC CTGCAGGGTA TCTCTGAGAC CGGCTTCTTG
    GATGACGTGT CATATGACAG CATATCCCTG GGTCCTGGAT ATGACCGGCC ATATCAGTTC
    AACCCCAATG TTCGAGAACC CAGAACCATA CAGCTGTACA AGCATCTCAA TCTGAAGAGC
    TGCATCTGGA CATTCGATGC TTATTATGAC ATGACGGAGC TTATCGATGT TTGCGGAGGA
    TCAGTAACTG CCGACTTTCA GGTCAGAGAC TCGGCTCAGT CCTTCCTGAC AGTCCAGGTT
    CCGCTCTATG TGTCCTACAT CTACGTGACG GCTCCTCGTG GATGGGCGTC TCTGGAGCAC
    CACACAGAAA TGGAGTTCTC CTTCTTCTAC GACACTGTCT TATGGAGAAC AGGCATTCAG
    ACAGACAGCG TGCTTTCGGC CAGATTACAG ATCATTCGGA TCTTCATTAA GGATGACGGA
    CGACTGGTGA TTGAGTTTAA AACCCACGCC AAGTTCAGAG GTCAGTTTGT CCCAGAGCAT
    CACACCCTCC CCGGACAGAA GTCTCATCTG ATGGCTCCAG ATCACTTAGG AGGCATGGAG
    TTCGACCTAC AGCTGGTGTG GAGCGCACAG TCTTTTGATT CACCCTATCA GCTCTGGAGA
    GCCACAAGCT CCTACAGCAG GAAAGACTAT TCAGGGGAGT ACACCGTCTT CCTCATTCCC
    TGCACGGTAC AGCCAACACA GGCATGGGTG GACTCTGGGG ACAAGCCTCT ATCCTGCACA
    GCACATGCAC CAGAGAAATT CCTGCTGCCC ATCGCCTTCC AGCAGACCAA CCGCCCTGTC
    CCTGTTGTCT ACTCTCTGAA TACTGAGTTC CAGCTTTGTA ATAATGAGAA AGTTTTTCTA
    ATGGATCCTG CTCTCGCAGA CATGTCTGTG GCTGAGATGG ATTACAAAGG AGCCTTTTCA
    ATGGGTCAAA CCCTGTATGG CAGAGTCCTG TGGAATCCTG ACCAGAACCT GAATTCAGCC
    TACAAGCTGC AGCTGGAGAA AGTGTATTTG TGTACGGGAC GGGATGGATA TGTTCCCTTC
    TTTGACCCTA CAGGCACACT GTATAATGAG GGCCCTCAGT ATGGCTGCAT TCAACCAAAC
    AAGCACCTTA AACACAGGTT CTTACTTCTG GACCGAAAGC AGCCTGATGT ATGTGATCGC
    TACTTCCATG ATGTTCCCTT TGAGGCCAGC TTTGCATCTG ACATTCCTGA CCTCCTGTCT
    TTGTCTGCGA TGCCCGGTGT AGACGGATTC ACAATGAAGG TTGATGCCCT TTATAAGGTT
    GAAGCAGGGC ACCAATGGTA CCTCCAGGTC ATCTACGTTA TTGGACCCGA GTCTATTTCG
    TCTCCCAGAA TCCAGCGCTC AGTGACTTAT GAGCTGAAGC GAAGCCGCCG AGATCTAGTA
    GACCGATCAG GTCGCCTAAT GCTTGATGAA TCATTGATCT ACGACAATGA AGGTGACCAG
    GTAAAGAATG GCACCAACAT GAAGACCTTG AAGCTTGAGA TGGAACCATC AGGCACCTTC
    AACCCCCAAA TGGGAGGATC CATTGGGGGT GGCGTGGCAG CCATCCTCCT GCTTTTCTTA
    GTCCTTCTAG CGTTCTGTTT CATGGTCAGG AAGTGCCGCA GGGTTGTCAA AAAGAGGCCT
    TCAAAGACGG TGGAGGATTA TCCTCTGAAC ACAAAGGTGG AGGTCTGCAT TGAACGTCTG
    GAGAAAAACC TCAACAGCAA GCACTGCTCT GTAAGAAACA TCAACGTTCT CAACAAGAAC
    CCCGACACCT ACAAAGTCAA TGGGGTGAAA GTCAAACAGG TCAATCTGGA GGTCAAACTC
    CACAACAATC TAAATGACGG CACTGAAGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001124312.1
    CDS1..11913
    Translation

    Target ORF information:

    RefSeq Version NM_001130840.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    ATGCCCTCAG ACACTGAGGA ACGCCCACCC CGGGAGACAC AGAGCCCCCC ACCAGCATAC 
    GTGGTGAGGT GGTCTCTCCC TTTCTCCGCG GACCCTGGTT CAAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGAAAAA CTGGTCCATT TGCCTGGAAA GTGCTGCCCA GAGTAATCTA
    GAGATTGTCA GAGAAGGGTT GTGTCGAGAG TGCCAGTGTC AAGAGGGTCA CGTGACCTGC
    TACCAGCACT CCTGTCCCAC CTGTCCACTG GGAACACTAA CTATACCTCA CCGGGAGCAG
    TGCTGTCCCG ACTGCAATCC AGAAGTAAAG TGCCACGAGG ACTGTCTGAC ATGCTCTGGC
    ACACCGGATC ACTGTGCCAG CTGTCGAGAC CCTTCTGCCC TGCTAATGAA CGGACGCTGT
    CTGCAGGCCT GTCCTCAAGG CTTTTTCGCT CAGGGCAAGG TTTGTGCAGC CTGCCAGCCG
    TCATGTGCCA CCTGTGAGAA TGACTTTGAA TGCACAGCGT GCGGTGGGTC GTTTCTGCTC
    AGTGGCAGGC AGTGTGTGGC CACCTGTGAG AGAGGTCTGT TTCAGGCCCA CACACACTGC
    CTCAGTTGCC ATGACTTATG CTCATCGTGT CAGGGGGCGG GGCCTCAGGA TTGTTTGACA
    TGCTCAGACT CCTCCCACTT ACTGAAGGAT GGATACTGTG TTTCAGACTG CGGCCCAGGC
    TTTTACACAA GTCAAGGCAA CTCTTGTGAC CCTTCCTGTG CAACCTGCCA ACCTGACAGC
    CCCAGCTGTA TGAGCTGCCC TGCAGAACAT GCACTGCATC AAGGGAAATG TGCACCACAA
    TGTCCAGCGC AGTATTACAA GGATGACCAC GGCCGATGCC AAGCTTGCCA CAGTTCGTGT
    GCGTCCTGCT CGGGGCCTGC TGTGTCTCAC TGCACCTCCT GCTCAAAGGC TCTGATCTTG
    AACCAGGGCC AGTGTGTGGA GTCCTGTGGA GAGGGCCTGT TCTCCAGAGC GGGACACTGT
    CACAGTTGTC ATTCGTCCTG TAGGGTCTGT GTGGGTCCAG ACTCCTCAGA CTGCACACAG
    TGTGTCAAAA TGGAAGAGGT GCTGCAGCCT CAAAATGGAC ATGTCTCTCA CGGGTCCTGC
    ATGTCCGCCT GCAGGCCACA GCATTACCTT GAAACAGATC GGACCTGCAG AGAATGCCAC
    AGCTCATGTT CAGGCTGCAT TGGTGGAAGT TTTCAGAACT GTACCTCCTG TTTGCGACCC
    GGTGTCCTTC ATCAAGGGCA GTGCCTCAGC AAATGTCCCA ATGGCTTCTT CCACATTCAG
    GACCTCACTT CTTGTCATCC ATCATGTAAG GAGTGCACTG GCACATCTCA GGCCGACTGT
    TCTGCCTGTC CCGCTCATGC CAGTCTTCAT AACGGTTACT GCCGAACCAG CTGCCCCGAG
    GGCCAGTACC TCAATTCTGT GGGTTTCTGC GATGAGTGCG AGGAGGGATG CCAGCGCTGT
    GTCGCAGACC TGCAGTCTGG CTCTGGCACT GTGTGTTTGT GGTGTAAAGT TCCCAGAATG
    CTTCTGCTGG GCAATCACTG TGTTCCCGAG TGCCCACACA AACACTACCG CTGGCATGGA
    GCCTGCAAAA AATGTCACTC TTCCTGTGAG GAGTGCAGTG CTGATGGTCC TCTTTCTTGT
    ACCTCTTGTT TGGCCCCTGA AGTTTTGGCC CCATCAGGAC TCTGTAGTCC ACGCTGTCCG
    ACTGGATATT ATGCTGATGA AAACCGAGTT TGCCAAAAGT GTGATTCTCA GTGTCTGAGC
    TGTGAGATGG CAGGCGTGTG CTCGTCCTGT AGAGATCCAG CTAAAGTGCT TCTGTTTGGG
    GAGTGTCAGT ATGAGAGCTG TGCTCATCAG TATTACCTCA ACACCAGCAC ACGCACATGC
    AGAGAATGCG ACTGGAGCTG TAATGCGTGC CGTGGACCTC TGCGCAGTGA CTGCCTGCAG
    TGCATGGAGG GATACGTCCT GCAGGATGGC ATTTGTGTGC CGGGTTGCTC ACCGGGATTC
    TACCAGGATG CAGAGAGATG TCTGAGCTGT GAAGAGCACT GTGAGCAGTG CCAGGCTCAT
    GGTCAGTGCC AGCGGTGCCA GTTGCCGTAT GCCTCATTTA ACGGACATTG TGTGCTGGAA
    TGCAGAAAGC ACTATTTTCT AGATGACTCG ACTCGACAGT GCACCGCATG CTCAGCTGGT
    TGTGTGGAGT GTGTGAGTGC AGGCGAGTGT CGCGTCTGTG CTGAAAACAC CTTCCTGAAG
    GATGGCAGAT GCATCCCAGA CTGTGGTCAT GGATATTATG CTGACCTAAA AACAAGAGCT
    TGTCATGCAA ATAGTCGTGC TCCTTCTCTT TACATCAATG GCTCTCTGCT GGTTCCTATT
    GGTGGAGTTA AGCCTCTGGA TCCAGCACTC CTGTCAATTA AGGATGCAGA TAGCCAATCA
    GAGAGCATGT TGCTTCAGCT CCTTCAGCCG CCCAGTAATG GCCACCTCAG GCTGCTGGAG
    AATGACAGAG AGAGATCGCT GGGCAAAGAT GACACTTTCA CCTATACTCA GCTCAAACGC
    AGAGCGGTGC GCTTCATTCA CGACAAAGAG CAGACCAAAA GTGGGCAGTT CACGTTAAGG
    GCAGCAGATC CTCAACTGTT CTCTCAGCCT CAGACTGTTA CTGTGCAGGC TCTCTCTCAG
    CAGCCACCCA CTGTAATCAC CAACCAAATC CTGTACATTA ACTCTGGCGA GACAGCCGTC
    ATCAGCAGAT CTATTCTACA CATCAGCGAC CTTGACAATC CTCAGGACGT GTTCATCACG
    GTTCTGGATC CACCTCAGCA CGGTCGCCTG ACATACATGC ACAGTGATAC GCAGGTCACC
    CATTTTAAAC TGGAAGAGCT AGATGGCGAG CAGCTGCAGT ATGTTCATGA TCGTTCAGAT
    GGAGACCAGG ACAGACTTCT GCTGCAGGTC AATGACGGAC ACAGCTACCA GAACGTCCTG
    TTCCACATCA GCATTGAACA GAAGGTTGGA TTGACTCCGC ACCTGCGTTC AGTGCCCAAA
    ACTTGGGTGA AGGAGGGAGG AATGGTGCAG CTTTCTAAAA AGTGTCTCAA TGCTCAATTC
    AAAGGAGCCA GTGACTCTGA GATCCTGTAC AGCATCCATT CTGAGAGCGG ACAGCCCAAA
    TACGGAGAGA TTGTGCTGGT GTCTATGCCC GCTGACGGTC CTGTGGAAGC GTGGCAAGCC
    TTTTCTGATG GTCAAGACTC TACACCCACC AGCTCATTTA CACAGCAAGA TGTAAATGAA
    GGCACTGTCT GGTACCGACA TTATGGATCT GGAACACAGA GAGACACTTT TCAGTTTCAG
    GTTTCAAGTG AGATCTATCC AGAGACTCTG AGCGATACAC AGACCTTCAC TATAGGAGTG
    ATGCCTCAGA GCCCTGGCAT GCCTCAGCTT TCACCTGGAG TCGATCTGCA GATCACTGCG
    CTGGAAGATC GCGTGACTGT GATCTCTCCA TCTGCTCTCT CCTTCACTGA CTCTGAGACA
    CCCAGCGACA AGCTTGTCTA CAACATCACC AAAGCGCTCA CCTCAGGCCA CGGTGTGCTG
    GAGCACAAGG ACAGACCTTA TCATCATGTG CGTCTCTTCA CCCAGGCTGA CGTAGATTCT
    GGCAAAATTA TCTACAGACC CCCACAGGCC CCATCTCATC TTCAGGAGCT TTATCAATAC
    TCATTTACAG GTCTGCCAGA ATCAGTGAGC GTCCATTTCA CAGTGTCTGG TGGAGAACAC
    ACCACCCCAG AGATGGACTT TGCCATCCTG CTTTTGGTCA ATCATCAGCA GCCACCAGTC
    TTTCAGATCC TGGACCCAGT GCTGGAGGTC AGCCTTGGCG GCAGGGCACC AGTGGGTGTG
    CAACAGTTGG CAGTAACAGA TTCAGACACG GCCCCTGAAG AGCTGGAGTT TGAGGTGGTG
    GAGGGACCAC TACATGGGAC GCTCCTGAAA GTGGACCTTG GATCCCAGGT CCAGATGGTC
    AATGGGGACA CCTTTACCTC CAGTGATGTC ACCAGAAACA TCCTGCAGTA TGAACATGCA
    GGGCTCACCA CTGAAGAGGA CTCCATGACC TTCTCAGTGA CTGACGGGAT CTCTATGACC
    TCCATCGCGG TGCAGGTTTT GGTGTCAGAG GTCAAAGGCG ACGCACCCAA GCGTGACCCA
    AAGGCTTTTC TTTCCATGGA AGTTGCAGAG AAGAGCAGCA GCATCATTAG ACGTTCACAT
    TTAGCTTATA TGGATGATGC ATCTCCAGAT GAGAGGATTT TTGTGCAGTT AGTCTCTGTG
    CCAATGTATG GGATCCTTAC AAAAAATAAA GCTGGAGCAG ACTATGAGGA GCTTAGTGAA
    TATTCTTCCT TTACAATGGA GGACATCAAC AACCACAAAA TAAGGTATGT TACGTCCTTT
    GAAACTGGGA ACCAGCCTGT GACTGACATT TTCCACTTCG TCGTCTATGA TGGTGATAAC
    AATCGTCTGG AAAACCAGAT GTTCACTATC ACTATCACCA CTACCCAGAG GCAGCCACCT
    GTTGTCAGCG TCCGCAGTGG CATTAAAGTT CAGGAAGGTG GGAGAGTCCA GCTCTCAACC
    AATCACATTA TTGCATCTGA CCCAGACACA CCCAGAAAGG ACCTGCTGGT TTGGCTGATC
    AGACCACCTA AATTTGGCTT CATTGAGAAC ACAAAGCAAG CAGTGGTTGG TGAATCTCGC
    GTGATCAGCA CTGATGTCCC CTTCACTATT GAAGACCTCA ATTCAGACCA TATCTTCTAC
    GTGCAAAACA TTCGCCAATC CAATATTCGA CAGGATGTCT TCAGCTTTTA TATCAGTGAT
    GGATCAAGTC AAACTGAGGC TTTTGATGTC ACTATAGATA TTCAGCACAC CAAAGAGGAC
    CTAGTTCCAA TTATCTCTGT AAATAGCATC CACGTGGAGG AGAACTCTGG TGTGGTCATT
    ACCAACTCCT CCCTTAACGT ACTGGACCGG GACACGCCAG AGAATGAGCT TTTGCTTACC
    ATCATCAAGA AGCCTTCATA CGGGAAGATA CGCAGAAGGC AGTTTTACTC AGACCCTTTA
    GAGAATGGCA GGATTCTTCA GCAGGGATCC ACCTTCACCT ACCAGGACAT TCTTGATGAG
    CTGCTGGTCT ACACTCCAGA CGGACGTGGC TCTGGGACTG ATGAGATCCA GTTTTCAGTA
    ACAGACGGCA TATACACGGA GACGGGCCGT CTGGAGTTCA GTATGGATAT TAGGAAGAAA
    GAAGGCCCTA GAGTAACAAT CAACAGAGGA CTGCAGCTCG CAGCAGGTTC TTCATCTAAA
    ATCACGGAGC AGCATCTGAA AGGAACGGAC ATAGATTCAG ATAACCTAAA GCTGAGGTTC
    ACCCTCATCA AAGACCTAAG TGTGGGCTCC CTGATGCTGA AAACAGGCAG AAACAAGGCT
    CGCGTATCCG TGAAAGGACC CGTCACCAGC TTCACCCAGG ATGACATCAA CAAAGGACAT
    GTGGAATACA TTCATGAAAA AGGAGAGAGC GGAGGAAGCT TCTCTTTTAA GTTCAACCTA
    GAAGATCCTG AGGGAAACAG AGTCATTGAC CAGTCTTTCT TCATTAGTGT TCTGGAGGAC
    AGACTTCCTC CCACAGTGGT GGTTAATAAG GGTTTAGTTT TGGATGAGAA CTCCTTGAAG
    AAGATCACAA CTCTGCAGCT CTCCTCTACT GATCAGGATA GTGAACCCGA TGAACTCGTT
    TACCGAATCA CTAAACAGCC CCAGCTTGGT CATCTAGAAC ATTCTAACAA ACCAGGAACA
    CGGATCAGCT CGTTTACCCA GGCTGACCTG GCCTCTCGCA GCATTCAGTA TGTGCACACC
    AGTGAAGACG AGATTCACTC CGATGACTTC ACCTTCACTG TATCTGATGG AGGAAACGAG
    GTCTCACAGA CGTTCTACAT CACCATCCGG CCGGTGGACG ATGCTCTGCC TGTGTTGCAG
    GTGCCCGGCA TGAGGGTGCA GGAGGGCGTG AGGAAGACCA TCACTGAGTT TGAGCTCAAG
    GCCACCGATG CTGACACTGA GGAGGACTCT GTCACTTTCA CCATTGTTCA GGCACCTCGT
    CATGGGACCA TCGAGCGCAC CAGTAATGGT CAGCATTATC GTCAGACAAA CACCTTCACT
    ATGGACGACG TCTACCAGAA CCGCATCAGT TACAACCATG ATGGCAGTAA CTCCCTCAAA
    GATCGATTCA CCTTCACTGT CGGTGACGGC ACCAACATGT TCTTCATCAT CGAGGAGGGT
    GGAAAAGAGG TTGTCACTGC TGCTCCTCAA AAGTTTAAAA TTGACATTTT ACCTGTTGAT
    GACGGGACCC CTCGTATTGT TACCAACCTG GGCCTGCAGT GGCTGGAGTA CATGGAAAAT
    AAGGCCACTA ATCTGATCTC CAAGAAGGAG CTTTTCACTA TCGATCCAGA CACAGATGAT
    GGACAGCTGA CTTATGAAAT CACTACAGAA GCTAAGCACG GCTACCTGGA GAGTAAACTC
    AATCCTGGCA AACCTATCAC CTCTTTCACA CAAGCTGACA TAAACCTTGG CCTTATCCGA
    TACGTTCTAA GCGAGGACAA TGTTCAGGAG ACCATGGACA ACTTCAAGTT CCTTGTAAAG
    GACAGCAAAC CTAACATCGT CAGTGACAAT GTGTTCCACA TCCAGTGGTC TCTGATCAGC
    TTTGAACACA AGAGTTTTAA CGTGAGTGAG AAGGATGGAA CGGTGGCGGT CACAGTGAAG
    AGGACTGGTA ATCTGAACCA GTATGCTATT GTGCTGTGTC GAACTGAGCA GGGCACTGCC
    ACCTCCACCT CCAGCATGGG CTCTCGGGCC GGACAGCAGG ATTATGTTGA ATACGCCGGA
    CAGGTTCAGT TTGATGAGAG AGAAGACACC AAGGTGTGCA CCATTGTGAT CAACGATGAC
    CAGGTGTTTG AGAACATCGA GAGCTTTAAT GTGGAGCTGA GTATGCCGGT GTATGCCCTC
    CTGGGCTCAG TGACCCGAGC TAAAGTCAAT ATCAATGACA CGGAGGATGA GCCCACTTTG
    CAGTTTGATA AGAAAACCTA CCACGTCAAT GAGAGCACTG GTTTCATATT TGCTCCTGTT
    GAGAGAAAAG GTGACACCAG CAGCACAGTT TCTGCCCTGT GCTACACTGT GCCTAAATCA
    GCTCGGGGCA GCAGTCTTCA CGCTCTCGAG TCCGGCTCAG ACTACAAGAC CCGTGGCATG
    AGTGGCGACA ACAGACTCAT CTTTGGTCCA GGAGTGAGCA TGTCCACCTG TGACGTCAAG
    CTGATCGATG ACAGCGAGTA TGAACTCTCT GAGGAGTTTG AGCTGGTGCT GTCAGACGCC
    TCAGATAATG CAAGGATGGG CGACGTCACT GTGGCCAAAG TCATCATCGA CGGGCCCAAC
    GACGCCTCTA CTGTGTTTCT GGGAAACGCA TCATACACCT TCAGTGAAGA TGCAGGCACC
    ATTGAAATCC CTGTTCTGCG GCAGGGCAGT GATCTTTCAT CAGTCACCTC AGTGTGGTGC
    GCCACACGTC CTGCAGAACA AGATTCAGCC ACGCCTGGAG TCGACTACAT CCCCAGCTCC
    AAAAAAGTAG AGTTCAAACC AGGCAAGACC GAGGAGACAT GCAGTTTGAC CATAATGGAT
    GACATCCAGA ACCCAACAAT AGAAGGGAAA GAATCCTTCA TTGTGTTTCT TAGCTCTGCT
    AATGGAGCGG TCCTGACTGA ACCCTATGAA GCCTCTGTCT ACATCACTGA CACCACTCAA
    GACATCCCAA GCATGCAGTT TGACAAGCTG GCATATACAG TAAAGGAAAA AGATGGCGTC
    CTGCACATCC CTATTTTTAG GACAGGAGAT CTGTCCTATA AGTCTTCTGT CCGCTGTTTC
    ACCCGCACAA TGTCTGCAAT GGTTATGGAT GACTTTGAGG AGAGGAGAAA TGCAGATGAT
    TCGAGAATCA CCTTTCAGAA AGGGGAGAAG GTGAAGAATT GTACAGTACT GATCCAGGAT
    GACTCCGTGT TTGAGCCTGA AGAGGAGTTC CAGGTCCACC TTGGAAGCCC ACAGGGTGAC
    CATTGGATTG GAGCCATGAT TGGAGTCAAC GACATTGTGA CTGTGACCAT CACAAATGAT
    GAAGATGCAC CTACGATCGA GTTTGAACAA GCCACATATC AAGTTCGTGA GCCACCTGGT
    CCCGATGGCA TTGAGGTTTT GAATATCAAG GTAATTCGTA AAGGAGACCT CGACCGCACC
    TCCAAAATTC GATGCAGCAC GAGGGATGGA TCGGCCCAGT CAGGTGTGGA CTATAACCCA
    AAGAGTCGAG TGCTGAAGTA CAGCCCTGGT GTGGACCACA TCATGTTTAA GGTGGAGATT
    CTGTCCAATG AGGACAGAGA GTGGCATGAG TCTTTCTCTT TGGTCCTTGG TCCTGACGAC
    CCAGTTGAAG CTGTTCTTGG AGAGATCATC ATTGCAACAG TCACTATTCT GGATCAGGAG
    GCTGCAGGAA GTCTTATCCT TCCGGCTCCA CCCATTGTGG TGTCTCTATC AGATTATGAC
    TACGTTCAGG AGGTCACTAA AGAGGGCAGC AAGAAGTCTC CGTCTCCTGG ATATCCGCTG
    GTCTGTGTGA CCCCCTGTGA CCCCCACTAC CCCAAATACT CCGTCATGAA GGAGCGCTGT
    GAGGAGGCAG GCATCAACCA GACTTCTCTA CACTTCAGCT GGGAGGTGGC AACTCCTACA
    GACACCAGCG GAGCAAGATC TCCCTTCGAG ACTGTGACCG ACAACACACC CTACACCACA
    ATCAACCACA TGGTCCTGGA CAGCATTTAT TTCAGCAGGC GCTTCCATGT GCGCTGTGTA
    GCCCAAGCCA GAGATAAAGC CGGACACCTG GGAACTCCAC TGAGGAGCAA CATTGTCACC
    ATTGGCACTG AGGGATCAAT ATGCCACACT CCAGTCACCA CAGGGACCGC TCGAGGCTTC
    CAAGCCCAGT CCTTTATCGC CACTCTAAAA TACCTGGATG TCAAACACAA AGAGCATCCA
    AACAGGATCC ACATTTCAGT TCAGATTCCC CATCAGGATG GGATGCTTCC ACTGGTGTCC
    ACCATGCCTC TGCATAACCT GCACTTCCTG CTCTCTGAGT CCATTTACCG TCAGCAACAC
    GTCTGCTCCA ACCTGGTCAC ACTTAAAGAC CTGCAGGGTA TCTCTGAGAC CGGCTTCTTG
    GATGACGTGT CATATGACAG CATATCCCTG GGTCCTGGAT ATGACCGGCC ATATCAGTTC
    AACCCCAATG TTCGAGAACC CAGAACCATA CAGCTGTACA AGCATCTCAA TCTGAAGAGC
    TGCATCTGGA CATTCGATGC TTATTATGAC ATGACGGAGC TTATCGATGT TTGCGGAGGA
    TCAGTAACTG CCGACTTTCA GGTCAGAGAC TCGGCTCAGT CCTTCCTGAC AGTCCAGGTT
    CCGCTCTATG TGTCCTACAT CTACGTGACG GCTCCTCGTG GATGGGCGTC TCTGGAGCAC
    CACACAGAAA TGGAGTTCTC CTTCTTCTAC GACACTGTCT TATGGAGAAC AGGCATTCAG
    ACAGACAGCG TGCTTTCGGC CAGATTACAG ATCATTCGGA TCTTCATTAA GGATGACGGA
    CGACTGGTGA TTGAGTTTAA AACCCACGCC AAGTTCAGAG GTCAGTTTGT CCCAGAGCAT
    CACACCCTCC CCGGACAGAA GTCTCATCTG ATGGCTCCAG ATCACTTAGG AGGCATGGAG
    TTCGACCTAC AGCTGGTGTG GAGCGCACAG TCTTTTGATT CACCCTATCA GCTCTGGAGA
    GCCACAAGCT CCTACAGCAG GAAAGACTAT TCAGGGGAGT ACACCGTCTT CCTCATTCCC
    TGCACGGTAC AGCCAACACA GGCATGGGTG GACTCTGGGG ACAAGCCTCT ATCCTGCACA
    GCACATGCAC CAGAGAAATT CCTGCTGCCC ATCGCCTTCC AGCAGACCAA CCGCCCTGTC
    CCTGTTGTCT ACTCTCTGAA TACTGAGTTC CAGCTTTGTA ATAATGAGAA AGTTTTTCTA
    ATGGATCCTG CTCTCGCAGA CATGTCTGTG GCTGAGATGG ATTACAAAGG AGCCTTTTCA
    ATGGGTCAAA CCCTGTATGG CAGAGTCCTG TGGAATCCTG ACCAGAACCT GAATTCAGCC
    TACAAGCTGC AGCTGGAGAA AGTGTATTTG TGTACGGGAC GGGATGGATA TGTTCCCTTC
    TTTGACCCTA CAGGCACACT GTATAATGAG GGCCCTCAGT ATGGCTGCAT TCAACCAAAC
    AAGCACCTTA AACACAGGTT CTTACTTCTG GACCGAAAGC AGCCTGATGT ATGTGATCGC
    TACTTCCATG ATGTTCCCTT TGAGGCCAGC TTTGCATCTG ACATTCCTGA CCTCCTGTCT
    TTGTCTGCGA TGCCCGGTGT AGACGGATTC ACAATGAAGG TTGATGCCCT TTATAAGGTT
    GAAGCAGGGC ACCAATGGTA CCTCCAGGTC ATCTACGTTA TTGGACCCGA GTCTATTTCG
    TCTCCCAGAA TCCAGCGCTC AGTGACTTAT GAGCTGAAGC GAAGCCGCCG AGATCTAGTA
    GACCGATCAG GTCGCCTAAT GCTTGATGAA TCATTGATCT ACGACAATGA AGGTGACCAG
    GTAAAGAATG GCACCAACAT GAAGACCTTG AAGCTTGAGA TGGAACCATC AGGCACCTTC
    AACCCCCAAA TGGGAGGATC CATTGGGGGT GGCGTGGCAG CCATCCTCCT GCTTTTCTTA
    GTCCTTCTAG CGTTCTGTTT CATGGTCAGG AAGTGCCGCA GGGTTGTCAA AAAGAGGCCT
    TCAAAGACGG TGGAGGATTA TCCTCTGAAC ACAAAGGTGG AGGTCTGCAT TGAACGTCTG
    GAGAAAAACC TCAACAGCAA GCACTGCTCT GTAAGAAACA TCAACGTTCT CAACAAGAAC
    CCCGACACCT ACAAAGTCAA TGGGGTGAAA GTCAAACAGG TCAATCTGGA GGTCAAACTC
    CACAACAATC TAAATGACGG CACTGAAGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10722
    Clone ID Related Accession (Same CDS sequence) XM_005165335.3 , XM_005165335.4
    Accession Version XM_005165335.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product extracellular matrix protein FRAS1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165335.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGC AGTGGTTGGT GAATCTCGCG TGATCAGCAC TGATGTCCCC
    TTCACTATTG AAGACCTCAA TTCAGACCAT ATCTTCTACG TGCAAAACAT TCGCCAATCC
    AATATTCGAC AGGATGTCTT CAGCTTTTAT ATCAGTGATG GATCAAGTCA AACTGAGGCT
    TTTGATGTCA CTATAGATAT TCAGCACACC AAAGAGGACC TAGTTCCAAT TATCTCTGTA
    AATAGCATCC ACGTGGAGGA GAACTCTGGT GTGGTCATTA CCAACTCCTC CCTTAACGTA
    CTGGACCGGG ACACGCCAGA GAATGAGCTT TTGCTTACCA TCATCAAGAA GCCTTCATAC
    GGGAAGATAC GCAGAAGGCA GTTTTACTCA GACCCTTTAG AGAATGGCAG GATTCTTCAG
    CAGGGATCCA CCTTCACCTA CCAGGACATT CTTGATGAGC TGCTGGTCTA CACTCCAGAC
    GGACGTGGCT CTGGGACTGA TGAGATCCAG TTTTCAGTAA CAGACGGCAT ATACACGGAG
    ACGGGCCGTC TGGAGTTCAG TATGGATATT AGGAAGAAAG AAGGCCCTAG AGTAACAATC
    AACAGAGGAC TGCAGCTCGC AGCAGGTTCT TCATCTAAAA TCACGGAGCA GCATCTGAAA
    GGAACGGACA TAGATTCAGA TAACCTAAAG CTGAGGTTCA CCCTCATCAA AGACCTAAGT
    GTGGGCTCCC TGATGCTGAA AACAGGCAGA AACAAGGCTC GCGTATCCGT GAAAGGACCC
    GTCACCAGCT TCACCCAGGA TGACATCAAC AAAGGACATG TGGAATACAT TCATGAAAAA
    GGAGAGAGCG GAGGAAGCTT CTCTTTTAAG TTCAACCTAG AAGATCCTGA GGGAAACAGA
    GTCATTGACC AGTCTTTCTT CATTAGTGTT CTGGAGGACA GACTTCCTCC CACAGTGGTG
    GTTAATAAGG GTTTAGTTTT GGATGAGAAC TCCTTGAAGA AGATCACAAC TCTGCAGCTC
    TCCTCTACTG ATCAGGATAG TGAACCCGAT GAACTCGTTT ACCGAATCAC TAAACAGCCC
    CAGCTTGGTC ATCTAGAACA TTCTAACAAA CCAGGAACAC GGATCAGCTC GTTTACCCAG
    GCTGACCTGG CCTCTCGCAG CATTCAGTAT GTGCACACCA GTGAAGACGA GATTCACTCC
    GATGACTTCA CCTTCACTGT ATCTGATGGA GGAAACGAGG TCTCACAGAC GTTCTACATC
    ACCATCCGGC CGGTGGACGA TGCTCTGCCT GTGTTGCAGG TGCCCGGCAT GAGGGTGCAG
    GAGGGCGTGA GGAAGACCAT CACTGAGTTT GAGCTCAAGG CCACCGATGC TGACACTGAG
    GAGGACTCTG TCACTTTCAC CATTGTTCAG GCACCTCGTC ATGGGACCAT CGAGCGCACC
    AGTAATGGTC AGCATTATCG TCAGACAAAC ACCTTCACTA TGGACGACGT CTACCAGAAC
    CGCATCAGTT ACAACCATGA TGGCAGTAAC TCCCTCAAAG ATCGATTCAC CTTCACTGTC
    GGTGACGGCA CCAACATGTT CTTCATCATC GAGGAGGGTG GAAAAGAGGT TGTCACTGCT
    GCTCCTCAAA AGTTTAAAAT TGACATTTTA CCTGTTGATG ACGGGACCCC TCGTATTGTT
    ACCAACCTGG GCCTGCAGTG GCTGGAGTAC ATGGAAAATA AGGCCACTAA TCTGATCTCC
    AAGAAGGAGC TTTTCACTAT CGATCCAGAC ACAGATGATG GACAGCTGAC TTATGAAATC
    ACTACAGAAG CTAAGCACGG CTACCTGGAG AGTAAACTCA ATCCTGGCAA ACCTATCACC
    TCTTTCACAC AAGCTGACAT AAACCTTGGC CTTATCCGAT ACGTTCTAAG CGAGGACAAT
    GTTCAGGAGA CCATGGACAA CTTCAAGTTC CTTGTAAAGG ACAGCAAACC TAACATCGTC
    AGTGACAATG TGTTCCACAT CCAGTGGTCT CTGATCAGCT TTGAACACAA GAGTTTTAAC
    GTGAGTGAGA AGGATGGAAC GGTGGCGGTC ACAGTGAAGA GGACTGGTAA TCTGAACCAG
    TATGCTATTG TGCTGTGTCG AACTGAGCAG GGCACTGCCA CCTCCACCTC CAGCATGGGC
    TCTCGGGCCG GACAGCAGGA TTATGTTGAA TACGCCGGAC AGGTTCAGTT TGATGAGAGA
    GAAGACACCA AGGTGTGCAC CATTGTGATC AACGATGACC AGGTGTTTGA GAACATCGAG
    AGCTTTAATG TGGAGCTGAG TATGCCGGTG TATGCCCTCC TGGGCTCAGT GACCCGAGCT
    AAAGTCAATA TCAATGACAC GGAGGATGAG CCCACTTTGC AGTTTGATAA GAAAACCTAC
    CACGTCAATG AGAGCACTGG TTTCATATTT GCTCCTGTTG AGAGAAAAGG TGACACCAGC
    AGCACAGTTT CTGCCCTGTG CTACACTGTG CCTAAATCAG CTCGGGGCAG CAGTCTTCAC
    GCTCTCGAGT CCGGCTCAGA CTACAAGACC CGTGGCATGA GTGGCGACAA CAGACTCATC
    TTTGGTCCAG GAGTGAGCAT GTCCACCTGT GACGTCAAGC TGATCGATGA CAGCGAGTAT
    GAACTCTCTG AGGAGTTTGA GCTGGTGCTG TCAGACGCCT CAGATAATGC AAGGATGGGC
    GACGTCACTG TGGCCAAAGT CATCATCGAC GGGCCCAACG ACGCCTCTAC TGTGTTTCTG
    GGAAACGCAT CATACACCTT CAGTGAAGAT GCAGGCACCA TTGAAATCCC TGTTCTGCGG
    CAGGGCAGTG ATCTTTCATC AGTCACCTCA GTGTGGTGCG CCACACGTCC TGCAGAACAA
    GATTCAGCCA CGCCTGGAGT CGACTACATC CCCAGCTCCA AAAAAGTAGA GTTCAAACCA
    GGCAAGACCG AGGAGACATG CAGTTTGACC ATAATGGATG ACATCCAGAA CCCAACAATA
    GAAGGGAAAG AATCCTTCAT TGTGTTTCTT AGCTCTGCTA ATGGAGCGGT CCTGACTGAA
    CCCTATGAAG CCTCTGTCTA CATCACTGAC ACCACTCAAG ACATCCCAAG CATGCAGTTT
    GACAAGCTGG CATATACAGT AAAGGAAAAA GATGGCGTCC TGCACATCCC TATTTTTAGG
    ACAGGAGATC TGTCCTATAA GTCTTCTGTC CGCTGTTTCA CCCGCACAAT GTCTGCAATG
    GTTATGGATG ACTTTGAGGA GAGGAGAAAT GCAGATGATT CGAGAATCAC CTTTCAGAAA
    GGGGAGAAGG TGAAGAATTG TACAGTACTG ATCCAGGATG ACTCCGTGTT TGAGCCTGAA
    GAGGAGTTCC AGGTCCACCT TGGAAGCCCA CAGGGTGACC ATTGGATTGG AGCCATGATT
    GGAGTCAACG ACATTGTGAC TGTGACCATC ACAAATGATG AAGATGCACC TACGATCGAG
    TTTGAACAAG CCACATATCA AGTTCGTGAG CCACCTGGTC CCGATGGCAT TGAGGTTTTG
    AATATCAAGG TAATTCGTAA AGGAGACCTC GACCGCACCT CCAAAATTCG ATGCAGCACG
    AGGGATGGAT CGGCCCAGTC AGGTGTGGAC TATAACCCAA AGAGTCGAGT GCTGAAGTAC
    AGCCCTGGTG TGGACCACAT CATGTTTAAG GTGGAGATTC TGTCCAATGA GGACAGAGAG
    TGGCATGAGT CTTTCTCTTT GGTCCTTGGT CCTGACGACC CAGTTGAAGC TGTTCTTGGA
    GAGATCATCA TTGCAACAGT CACTATTCTG GATCAGGAGG CTGCAGGAAG TCTTATCCTT
    CCGGCTCCAC CCATTGTGGT GTCTCTATCA GATTATGACT ACGTTCAGGA GGTCACTAAA
    GAGGGCAGCA AGAAGTCTCC GTCTCCTGGA TATCCGCTGG TCTGTGTGAC CCCCTGTGAC
    CCCCACTACC CCAAATACTC CGTCATGAAG GAGCGCTGTG AGGAGGCAGG CATCAACCAG
    ACTTCTCTAC ACTTCAGCTG GGAGGTGGCA ACTCCTACAG ACACCAGCGG AGCAAGATCT
    CCCTTCGAGA CTGTGACCGA CAACACACCC TACACCACAA TCAACCACAT GGTCCTGGAC
    AGCATTTATT TCAGCAGGCG CTTCCATGTG CGCTGTGTAG CCCAAGCCAG AGATAAAGCC
    GGACACCTGG GAACTCCACT GAGGAGCAAC ATTGTCACCA TTGGCACTGA GGGATCAATA
    TGCCACACTC CAGTCACCAC AGGGACCGCT CGAGGCTTCC AAGCCCAGTC CTTTATCGCC
    ACTCTAAAAT ACCTGGATGT CAAACACAAA GAGCATCCAA ACAGGATCCA CATTTCAGTT
    CAGATTCCCC ATCAGGATGG GATGCTTCCA CTGGTGTCCA CCATGCCTCT GCATAACCTG
    CACTTCCTGC TCTCTGAGTC CATTTACCGT CAGCAACACG TCTGCTCCAA CCTGGTCACA
    CTTAAAGACC TGCAGGGTAT CTCTGAGACC GGCTTCTTGG ATGACGTGTC ATATGACAGC
    ATATCCCTGG GTCCTGGATA TGACCGGCCA TATCAGTTCA ACCCCAATGT TCGAGAACCC
    AGAACCATAC AGCTGTACAA GCATCTCAAT CTGAAGAGCT GCATCTGGAC ATTCGATGCT
    TATTATGACA TGACGGAGCT TATCGATGTT TGCGGAGGAT CAGTAACTGC CGACTTTCAG
    GTCAGAGACT CGGCTCAGTC CTTCCTGACA GTCCAGGTTC CGCTCTATGT GTCCTACATC
    TACGTGACGG CTCCTCGTGG ATGGGCGTCT CTGGAGCACC ACACAGAAAT GGAGTTCTCC
    TTCTTCTACG ACACTGTCTT ATGGAGAACA GGCATTCAGA CAGACAGCGT GCTTTCGGCC
    AGATTACAGA TCATTCGGAT CTTCATTAAG GATGACGGAC GACTGGTGAT TGAGTTTAAA
    ACCCACGCCA AGTTCAGAGG TCAGTTTGTC CCAGAGCATC ACACCCTCCC CGGACAGAAG
    TCTCATCTGA TGGCTCCAGA TCACTTAGGA GGCATGGAGT TCGACCTACA GCTGGTGTGG
    AGCGCACAGT CTTTTGATTC ACCCTATCAG CTCTGGAGAG CCACAAGCTC CTACAGCAGG
    AAAGACTATT CAGGGGAGTA CACCGTCTTC CTCATTCCCT GCACGGTACA GCCAACACAG
    GCATGGGTGG ACTCTGGGGA CAAGCCTCTA TCCTGCACAG CACATGCACC AGAGAAATTC
    CTGCTGCCCA TCGCCTTCCA GCAGACCAAC CGCCCTGTCC CTGTTGTCTA CTCTCTGAAT
    ACTGAGTTCC AGCTTTGTAA TAATGAGAAA GTTTTTCTAA TGGATCCTGC TCTCGCAGAC
    ATGTCTGTGG CTGAGATGGA TTACAAAGGA GCCTTTTCAA TGGGTCAAAC CCTGTATGGC
    AGAGTCCTGT GGAATCCTGA CCAGAACCTG AATTCAGCCT ACAAGCTGCA GCTGGAGAAA
    GTGTATTTGT GTACGGGACG GGATGGATAT GTTCCCTTCT TTGACCCTAC AGGCACACTG
    TATAATGAGG GCCCTCAGTA TGGCTGCATT CAACCAAACA AGCACCTTAA ACACAGGTTC
    TTACTTCTGG ACCGAAAGCA GCCTGATGTA TGTGATCGCT ACTTCCATGA TGTTCCCTTT
    GAGGCCAGCT TTGCATCTGA CATTCCTGAC CTCCTGTCTT TGTCTGCGAT GCCCGGTGTA
    GACGGATTCA CAATGAAGGT TGATGCCCTT TATAAGGTTG AAGCAGGGCA CCAATGGTAC
    CTCCAGGTCA TCTACGTTAT TGGACCCGAG TCTATTTCGT CTCCCAGAAT CCAGCGCTCA
    GTGACTTATG AGCTGAAGCG AAGCCGCCGA GATCTAGTAG ACCGATCAGG TCGCCTAATG
    CTTGATGAAT CATTGATCTA CGACAATGAA GGTGACCAGG TAAAGAATGG CACCAACATG
    AAGACCTTGA AGCTTGAGAT GGAACCATCA GGCACCTTCA ACCCCCAAAT GGGAGGATCC
    ATTGGGGGTG GCGTGGCAGC CATCCTCCTG CTTTTCTTAG TCCTTCTAGC GTTCTGTTTC
    ATGGTCAGGA AGTGCCGCAG GGTTGTCAAA AAGAGGCCTT CAAAGACGGT GGAGGATTAT
    CCTCTGAACA CAAAGGTGGA GGTCTGCATT GAACGTCTGG AGAAAAACCT CAACAGCAAG
    CACTGCTCTG TAAGAAACAT CAACGTTCTC AACAAGAACC CCGACACCTA CAAAGTCAAT
    GGGGTGAAAG TCAAACAGGT CAATCTGGAG GTCAAACTCC ACAACAATCT AAATGACGGC
    ACTGAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165392.1
    CDS365..12376
    Translation

    Target ORF information:

    RefSeq Version XM_005165335.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165335.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGC AGTGGTTGGT GAATCTCGCG TGATCAGCAC TGATGTCCCC
    TTCACTATTG AAGACCTCAA TTCAGACCAT ATCTTCTACG TGCAAAACAT TCGCCAATCC
    AATATTCGAC AGGATGTCTT CAGCTTTTAT ATCAGTGATG GATCAAGTCA AACTGAGGCT
    TTTGATGTCA CTATAGATAT TCAGCACACC AAAGAGGACC TAGTTCCAAT TATCTCTGTA
    AATAGCATCC ACGTGGAGGA GAACTCTGGT GTGGTCATTA CCAACTCCTC CCTTAACGTA
    CTGGACCGGG ACACGCCAGA GAATGAGCTT TTGCTTACCA TCATCAAGAA GCCTTCATAC
    GGGAAGATAC GCAGAAGGCA GTTTTACTCA GACCCTTTAG AGAATGGCAG GATTCTTCAG
    CAGGGATCCA CCTTCACCTA CCAGGACATT CTTGATGAGC TGCTGGTCTA CACTCCAGAC
    GGACGTGGCT CTGGGACTGA TGAGATCCAG TTTTCAGTAA CAGACGGCAT ATACACGGAG
    ACGGGCCGTC TGGAGTTCAG TATGGATATT AGGAAGAAAG AAGGCCCTAG AGTAACAATC
    AACAGAGGAC TGCAGCTCGC AGCAGGTTCT TCATCTAAAA TCACGGAGCA GCATCTGAAA
    GGAACGGACA TAGATTCAGA TAACCTAAAG CTGAGGTTCA CCCTCATCAA AGACCTAAGT
    GTGGGCTCCC TGATGCTGAA AACAGGCAGA AACAAGGCTC GCGTATCCGT GAAAGGACCC
    GTCACCAGCT TCACCCAGGA TGACATCAAC AAAGGACATG TGGAATACAT TCATGAAAAA
    GGAGAGAGCG GAGGAAGCTT CTCTTTTAAG TTCAACCTAG AAGATCCTGA GGGAAACAGA
    GTCATTGACC AGTCTTTCTT CATTAGTGTT CTGGAGGACA GACTTCCTCC CACAGTGGTG
    GTTAATAAGG GTTTAGTTTT GGATGAGAAC TCCTTGAAGA AGATCACAAC TCTGCAGCTC
    TCCTCTACTG ATCAGGATAG TGAACCCGAT GAACTCGTTT ACCGAATCAC TAAACAGCCC
    CAGCTTGGTC ATCTAGAACA TTCTAACAAA CCAGGAACAC GGATCAGCTC GTTTACCCAG
    GCTGACCTGG CCTCTCGCAG CATTCAGTAT GTGCACACCA GTGAAGACGA GATTCACTCC
    GATGACTTCA CCTTCACTGT ATCTGATGGA GGAAACGAGG TCTCACAGAC GTTCTACATC
    ACCATCCGGC CGGTGGACGA TGCTCTGCCT GTGTTGCAGG TGCCCGGCAT GAGGGTGCAG
    GAGGGCGTGA GGAAGACCAT CACTGAGTTT GAGCTCAAGG CCACCGATGC TGACACTGAG
    GAGGACTCTG TCACTTTCAC CATTGTTCAG GCACCTCGTC ATGGGACCAT CGAGCGCACC
    AGTAATGGTC AGCATTATCG TCAGACAAAC ACCTTCACTA TGGACGACGT CTACCAGAAC
    CGCATCAGTT ACAACCATGA TGGCAGTAAC TCCCTCAAAG ATCGATTCAC CTTCACTGTC
    GGTGACGGCA CCAACATGTT CTTCATCATC GAGGAGGGTG GAAAAGAGGT TGTCACTGCT
    GCTCCTCAAA AGTTTAAAAT TGACATTTTA CCTGTTGATG ACGGGACCCC TCGTATTGTT
    ACCAACCTGG GCCTGCAGTG GCTGGAGTAC ATGGAAAATA AGGCCACTAA TCTGATCTCC
    AAGAAGGAGC TTTTCACTAT CGATCCAGAC ACAGATGATG GACAGCTGAC TTATGAAATC
    ACTACAGAAG CTAAGCACGG CTACCTGGAG AGTAAACTCA ATCCTGGCAA ACCTATCACC
    TCTTTCACAC AAGCTGACAT AAACCTTGGC CTTATCCGAT ACGTTCTAAG CGAGGACAAT
    GTTCAGGAGA CCATGGACAA CTTCAAGTTC CTTGTAAAGG ACAGCAAACC TAACATCGTC
    AGTGACAATG TGTTCCACAT CCAGTGGTCT CTGATCAGCT TTGAACACAA GAGTTTTAAC
    GTGAGTGAGA AGGATGGAAC GGTGGCGGTC ACAGTGAAGA GGACTGGTAA TCTGAACCAG
    TATGCTATTG TGCTGTGTCG AACTGAGCAG GGCACTGCCA CCTCCACCTC CAGCATGGGC
    TCTCGGGCCG GACAGCAGGA TTATGTTGAA TACGCCGGAC AGGTTCAGTT TGATGAGAGA
    GAAGACACCA AGGTGTGCAC CATTGTGATC AACGATGACC AGGTGTTTGA GAACATCGAG
    AGCTTTAATG TGGAGCTGAG TATGCCGGTG TATGCCCTCC TGGGCTCAGT GACCCGAGCT
    AAAGTCAATA TCAATGACAC GGAGGATGAG CCCACTTTGC AGTTTGATAA GAAAACCTAC
    CACGTCAATG AGAGCACTGG TTTCATATTT GCTCCTGTTG AGAGAAAAGG TGACACCAGC
    AGCACAGTTT CTGCCCTGTG CTACACTGTG CCTAAATCAG CTCGGGGCAG CAGTCTTCAC
    GCTCTCGAGT CCGGCTCAGA CTACAAGACC CGTGGCATGA GTGGCGACAA CAGACTCATC
    TTTGGTCCAG GAGTGAGCAT GTCCACCTGT GACGTCAAGC TGATCGATGA CAGCGAGTAT
    GAACTCTCTG AGGAGTTTGA GCTGGTGCTG TCAGACGCCT CAGATAATGC AAGGATGGGC
    GACGTCACTG TGGCCAAAGT CATCATCGAC GGGCCCAACG ACGCCTCTAC TGTGTTTCTG
    GGAAACGCAT CATACACCTT CAGTGAAGAT GCAGGCACCA TTGAAATCCC TGTTCTGCGG
    CAGGGCAGTG ATCTTTCATC AGTCACCTCA GTGTGGTGCG CCACACGTCC TGCAGAACAA
    GATTCAGCCA CGCCTGGAGT CGACTACATC CCCAGCTCCA AAAAAGTAGA GTTCAAACCA
    GGCAAGACCG AGGAGACATG CAGTTTGACC ATAATGGATG ACATCCAGAA CCCAACAATA
    GAAGGGAAAG AATCCTTCAT TGTGTTTCTT AGCTCTGCTA ATGGAGCGGT CCTGACTGAA
    CCCTATGAAG CCTCTGTCTA CATCACTGAC ACCACTCAAG ACATCCCAAG CATGCAGTTT
    GACAAGCTGG CATATACAGT AAAGGAAAAA GATGGCGTCC TGCACATCCC TATTTTTAGG
    ACAGGAGATC TGTCCTATAA GTCTTCTGTC CGCTGTTTCA CCCGCACAAT GTCTGCAATG
    GTTATGGATG ACTTTGAGGA GAGGAGAAAT GCAGATGATT CGAGAATCAC CTTTCAGAAA
    GGGGAGAAGG TGAAGAATTG TACAGTACTG ATCCAGGATG ACTCCGTGTT TGAGCCTGAA
    GAGGAGTTCC AGGTCCACCT TGGAAGCCCA CAGGGTGACC ATTGGATTGG AGCCATGATT
    GGAGTCAACG ACATTGTGAC TGTGACCATC ACAAATGATG AAGATGCACC TACGATCGAG
    TTTGAACAAG CCACATATCA AGTTCGTGAG CCACCTGGTC CCGATGGCAT TGAGGTTTTG
    AATATCAAGG TAATTCGTAA AGGAGACCTC GACCGCACCT CCAAAATTCG ATGCAGCACG
    AGGGATGGAT CGGCCCAGTC AGGTGTGGAC TATAACCCAA AGAGTCGAGT GCTGAAGTAC
    AGCCCTGGTG TGGACCACAT CATGTTTAAG GTGGAGATTC TGTCCAATGA GGACAGAGAG
    TGGCATGAGT CTTTCTCTTT GGTCCTTGGT CCTGACGACC CAGTTGAAGC TGTTCTTGGA
    GAGATCATCA TTGCAACAGT CACTATTCTG GATCAGGAGG CTGCAGGAAG TCTTATCCTT
    CCGGCTCCAC CCATTGTGGT GTCTCTATCA GATTATGACT ACGTTCAGGA GGTCACTAAA
    GAGGGCAGCA AGAAGTCTCC GTCTCCTGGA TATCCGCTGG TCTGTGTGAC CCCCTGTGAC
    CCCCACTACC CCAAATACTC CGTCATGAAG GAGCGCTGTG AGGAGGCAGG CATCAACCAG
    ACTTCTCTAC ACTTCAGCTG GGAGGTGGCA ACTCCTACAG ACACCAGCGG AGCAAGATCT
    CCCTTCGAGA CTGTGACCGA CAACACACCC TACACCACAA TCAACCACAT GGTCCTGGAC
    AGCATTTATT TCAGCAGGCG CTTCCATGTG CGCTGTGTAG CCCAAGCCAG AGATAAAGCC
    GGACACCTGG GAACTCCACT GAGGAGCAAC ATTGTCACCA TTGGCACTGA GGGATCAATA
    TGCCACACTC CAGTCACCAC AGGGACCGCT CGAGGCTTCC AAGCCCAGTC CTTTATCGCC
    ACTCTAAAAT ACCTGGATGT CAAACACAAA GAGCATCCAA ACAGGATCCA CATTTCAGTT
    CAGATTCCCC ATCAGGATGG GATGCTTCCA CTGGTGTCCA CCATGCCTCT GCATAACCTG
    CACTTCCTGC TCTCTGAGTC CATTTACCGT CAGCAACACG TCTGCTCCAA CCTGGTCACA
    CTTAAAGACC TGCAGGGTAT CTCTGAGACC GGCTTCTTGG ATGACGTGTC ATATGACAGC
    ATATCCCTGG GTCCTGGATA TGACCGGCCA TATCAGTTCA ACCCCAATGT TCGAGAACCC
    AGAACCATAC AGCTGTACAA GCATCTCAAT CTGAAGAGCT GCATCTGGAC ATTCGATGCT
    TATTATGACA TGACGGAGCT TATCGATGTT TGCGGAGGAT CAGTAACTGC CGACTTTCAG
    GTCAGAGACT CGGCTCAGTC CTTCCTGACA GTCCAGGTTC CGCTCTATGT GTCCTACATC
    TACGTGACGG CTCCTCGTGG ATGGGCGTCT CTGGAGCACC ACACAGAAAT GGAGTTCTCC
    TTCTTCTACG ACACTGTCTT ATGGAGAACA GGCATTCAGA CAGACAGCGT GCTTTCGGCC
    AGATTACAGA TCATTCGGAT CTTCATTAAG GATGACGGAC GACTGGTGAT TGAGTTTAAA
    ACCCACGCCA AGTTCAGAGG TCAGTTTGTC CCAGAGCATC ACACCCTCCC CGGACAGAAG
    TCTCATCTGA TGGCTCCAGA TCACTTAGGA GGCATGGAGT TCGACCTACA GCTGGTGTGG
    AGCGCACAGT CTTTTGATTC ACCCTATCAG CTCTGGAGAG CCACAAGCTC CTACAGCAGG
    AAAGACTATT CAGGGGAGTA CACCGTCTTC CTCATTCCCT GCACGGTACA GCCAACACAG
    GCATGGGTGG ACTCTGGGGA CAAGCCTCTA TCCTGCACAG CACATGCACC AGAGAAATTC
    CTGCTGCCCA TCGCCTTCCA GCAGACCAAC CGCCCTGTCC CTGTTGTCTA CTCTCTGAAT
    ACTGAGTTCC AGCTTTGTAA TAATGAGAAA GTTTTTCTAA TGGATCCTGC TCTCGCAGAC
    ATGTCTGTGG CTGAGATGGA TTACAAAGGA GCCTTTTCAA TGGGTCAAAC CCTGTATGGC
    AGAGTCCTGT GGAATCCTGA CCAGAACCTG AATTCAGCCT ACAAGCTGCA GCTGGAGAAA
    GTGTATTTGT GTACGGGACG GGATGGATAT GTTCCCTTCT TTGACCCTAC AGGCACACTG
    TATAATGAGG GCCCTCAGTA TGGCTGCATT CAACCAAACA AGCACCTTAA ACACAGGTTC
    TTACTTCTGG ACCGAAAGCA GCCTGATGTA TGTGATCGCT ACTTCCATGA TGTTCCCTTT
    GAGGCCAGCT TTGCATCTGA CATTCCTGAC CTCCTGTCTT TGTCTGCGAT GCCCGGTGTA
    GACGGATTCA CAATGAAGGT TGATGCCCTT TATAAGGTTG AAGCAGGGCA CCAATGGTAC
    CTCCAGGTCA TCTACGTTAT TGGACCCGAG TCTATTTCGT CTCCCAGAAT CCAGCGCTCA
    GTGACTTATG AGCTGAAGCG AAGCCGCCGA GATCTAGTAG ACCGATCAGG TCGCCTAATG
    CTTGATGAAT CATTGATCTA CGACAATGAA GGTGACCAGG TAAAGAATGG CACCAACATG
    AAGACCTTGA AGCTTGAGAT GGAACCATCA GGCACCTTCA ACCCCCAAAT GGGAGGATCC
    ATTGGGGGTG GCGTGGCAGC CATCCTCCTG CTTTTCTTAG TCCTTCTAGC GTTCTGTTTC
    ATGGTCAGGA AGTGCCGCAG GGTTGTCAAA AAGAGGCCTT CAAAGACGGT GGAGGATTAT
    CCTCTGAACA CAAAGGTGGA GGTCTGCATT GAACGTCTGG AGAAAAACCT CAACAGCAAG
    CACTGCTCTG TAAGAAACAT CAACGTTCTC AACAAGAACC CCGACACCTA CAAAGTCAAT
    GGGGTGAAAG TCAAACAGGT CAATCTGGAG GTCAAACTCC ACAACAATCT AAATGACGGC
    ACTGAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10722
    Clone ID Related Accession (Same CDS sequence) XM_005165335.3 , XM_005165335.4
    Accession Version XM_005165335.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product extracellular matrix protein FRAS1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGC AGTGGTTGGT GAATCTCGCG TGATCAGCAC TGATGTCCCC
    TTCACTATTG AAGACCTCAA TTCAGACCAT ATCTTCTACG TGCAAAACAT TCGCCAATCC
    AATATTCGAC AGGATGTCTT CAGCTTTTAT ATCAGTGATG GATCAAGTCA AACTGAGGCT
    TTTGATGTCA CTATAGATAT TCAGCACACC AAAGAGGACC TAGTTCCAAT TATCTCTGTA
    AATAGCATCC ACGTGGAGGA GAACTCTGGT GTGGTCATTA CCAACTCCTC CCTTAACGTA
    CTGGACCGGG ACACGCCAGA GAATGAGCTT TTGCTTACCA TCATCAAGAA GCCTTCATAC
    GGGAAGATAC GCAGAAGGCA GTTTTACTCA GACCCTTTAG AGAATGGCAG GATTCTTCAG
    CAGGGATCCA CCTTCACCTA CCAGGACATT CTTGATGAGC TGCTGGTCTA CACTCCAGAC
    GGACGTGGCT CTGGGACTGA TGAGATCCAG TTTTCAGTAA CAGACGGCAT ATACACGGAG
    ACGGGCCGTC TGGAGTTCAG TATGGATATT AGGAAGAAAG AAGGCCCTAG AGTAACAATC
    AACAGAGGAC TGCAGCTCGC AGCAGGTTCT TCATCTAAAA TCACGGAGCA GCATCTGAAA
    GGAACGGACA TAGATTCAGA TAACCTAAAG CTGAGGTTCA CCCTCATCAA AGACCTAAGT
    GTGGGCTCCC TGATGCTGAA AACAGGCAGA AACAAGGCTC GCGTATCCGT GAAAGGACCC
    GTCACCAGCT TCACCCAGGA TGACATCAAC AAAGGACATG TGGAATACAT TCATGAAAAA
    GGAGAGAGCG GAGGAAGCTT CTCTTTTAAG TTCAACCTAG AAGATCCTGA GGGAAACAGA
    GTCATTGACC AGTCTTTCTT CATTAGTGTT CTGGAGGACA GACTTCCTCC CACAGTGGTG
    GTTAATAAGG GTTTAGTTTT GGATGAGAAC TCCTTGAAGA AGATCACAAC TCTGCAGCTC
    TCCTCTACTG ATCAGGATAG TGAACCCGAT GAACTCGTTT ACCGAATCAC TAAACAGCCC
    CAGCTTGGTC ATCTAGAACA TTCTAACAAA CCAGGAACAC GGATCAGCTC GTTTACCCAG
    GCTGACCTGG CCTCTCGCAG CATTCAGTAT GTGCACACCA GTGAAGACGA GATTCACTCC
    GATGACTTCA CCTTCACTGT ATCTGATGGA GGAAACGAGG TCTCACAGAC GTTCTACATC
    ACCATCCGGC CGGTGGACGA TGCTCTGCCT GTGTTGCAGG TGCCCGGCAT GAGGGTGCAG
    GAGGGCGTGA GGAAGACCAT CACTGAGTTT GAGCTCAAGG CCACCGATGC TGACACTGAG
    GAGGACTCTG TCACTTTCAC CATTGTTCAG GCACCTCGTC ATGGGACCAT CGAGCGCACC
    AGTAATGGTC AGCATTATCG TCAGACAAAC ACCTTCACTA TGGACGACGT CTACCAGAAC
    CGCATCAGTT ACAACCATGA TGGCAGTAAC TCCCTCAAAG ATCGATTCAC CTTCACTGTC
    GGTGACGGCA CCAACATGTT CTTCATCATC GAGGAGGGTG GAAAAGAGGT TGTCACTGCT
    GCTCCTCAAA AGTTTAAAAT TGACATTTTA CCTGTTGATG ACGGGACCCC TCGTATTGTT
    ACCAACCTGG GCCTGCAGTG GCTGGAGTAC ATGGAAAATA AGGCCACTAA TCTGATCTCC
    AAGAAGGAGC TTTTCACTAT CGATCCAGAC ACAGATGATG GACAGCTGAC TTATGAAATC
    ACTACAGAAG CTAAGCACGG CTACCTGGAG AGTAAACTCA ATCCTGGCAA ACCTATCACC
    TCTTTCACAC AAGCTGACAT AAACCTTGGC CTTATCCGAT ACGTTCTAAG CGAGGACAAT
    GTTCAGGAGA CCATGGACAA CTTCAAGTTC CTTGTAAAGG ACAGCAAACC TAACATCGTC
    AGTGACAATG TGTTCCACAT CCAGTGGTCT CTGATCAGCT TTGAACACAA GAGTTTTAAC
    GTGAGTGAGA AGGATGGAAC GGTGGCGGTC ACAGTGAAGA GGACTGGTAA TCTGAACCAG
    TATGCTATTG TGCTGTGTCG AACTGAGCAG GGCACTGCCA CCTCCACCTC CAGCATGGGC
    TCTCGGGCCG GACAGCAGGA TTATGTTGAA TACGCCGGAC AGGTTCAGTT TGATGAGAGA
    GAAGACACCA AGGTGTGCAC CATTGTGATC AACGATGACC AGGTGTTTGA GAACATCGAG
    AGCTTTAATG TGGAGCTGAG TATGCCGGTG TATGCCCTCC TGGGCTCAGT GACCCGAGCT
    AAAGTCAATA TCAATGACAC GGAGGATGAG CCCACTTTGC AGTTTGATAA GAAAACCTAC
    CACGTCAATG AGAGCACTGG TTTCATATTT GCTCCTGTTG AGAGAAAAGG TGACACCAGC
    AGCACAGTTT CTGCCCTGTG CTACACTGTG CCTAAATCAG CTCGGGGCAG CAGTCTTCAC
    GCTCTCGAGT CCGGCTCAGA CTACAAGACC CGTGGCATGA GTGGCGACAA CAGACTCATC
    TTTGGTCCAG GAGTGAGCAT GTCCACCTGT GACGTCAAGC TGATCGATGA CAGCGAGTAT
    GAACTCTCTG AGGAGTTTGA GCTGGTGCTG TCAGACGCCT CAGATAATGC AAGGATGGGC
    GACGTCACTG TGGCCAAAGT CATCATCGAC GGGCCCAACG ACGCCTCTAC TGTGTTTCTG
    GGAAACGCAT CATACACCTT CAGTGAAGAT GCAGGCACCA TTGAAATCCC TGTTCTGCGG
    CAGGGCAGTG ATCTTTCATC AGTCACCTCA GTGTGGTGCG CCACACGTCC TGCAGAACAA
    GATTCAGCCA CGCCTGGAGT CGACTACATC CCCAGCTCCA AAAAAGTAGA GTTCAAACCA
    GGCAAGACCG AGGAGACATG CAGTTTGACC ATAATGGATG ACATCCAGAA CCCAACAATA
    GAAGGGAAAG AATCCTTCAT TGTGTTTCTT AGCTCTGCTA ATGGAGCGGT CCTGACTGAA
    CCCTATGAAG CCTCTGTCTA CATCACTGAC ACCACTCAAG ACATCCCAAG CATGCAGTTT
    GACAAGCTGG CATATACAGT AAAGGAAAAA GATGGCGTCC TGCACATCCC TATTTTTAGG
    ACAGGAGATC TGTCCTATAA GTCTTCTGTC CGCTGTTTCA CCCGCACAAT GTCTGCAATG
    GTTATGGATG ACTTTGAGGA GAGGAGAAAT GCAGATGATT CGAGAATCAC CTTTCAGAAA
    GGGGAGAAGG TGAAGAATTG TACAGTACTG ATCCAGGATG ACTCCGTGTT TGAGCCTGAA
    GAGGAGTTCC AGGTCCACCT TGGAAGCCCA CAGGGTGACC ATTGGATTGG AGCCATGATT
    GGAGTCAACG ACATTGTGAC TGTGACCATC ACAAATGATG AAGATGCACC TACGATCGAG
    TTTGAACAAG CCACATATCA AGTTCGTGAG CCACCTGGTC CCGATGGCAT TGAGGTTTTG
    AATATCAAGG TAATTCGTAA AGGAGACCTC GACCGCACCT CCAAAATTCG ATGCAGCACG
    AGGGATGGAT CGGCCCAGTC AGGTGTGGAC TATAACCCAA AGAGTCGAGT GCTGAAGTAC
    AGCCCTGGTG TGGACCACAT CATGTTTAAG GTGGAGATTC TGTCCAATGA GGACAGAGAG
    TGGCATGAGT CTTTCTCTTT GGTCCTTGGT CCTGACGACC CAGTTGAAGC TGTTCTTGGA
    GAGATCATCA TTGCAACAGT CACTATTCTG GATCAGGAGG CTGCAGGAAG TCTTATCCTT
    CCGGCTCCAC CCATTGTGGT GTCTCTATCA GATTATGACT ACGTTCAGGA GGTCACTAAA
    GAGGGCAGCA AGAAGTCTCC GTCTCCTGGA TATCCGCTGG TCTGTGTGAC CCCCTGTGAC
    CCCCACTACC CCAAATACTC CGTCATGAAG GAGCGCTGTG AGGAGGCAGG CATCAACCAG
    ACTTCTCTAC ACTTCAGCTG GGAGGTGGCA ACTCCTACAG ACACCAGCGG AGCAAGATCT
    CCCTTCGAGA CTGTGACCGA CAACACACCC TACACCACAA TCAACCACAT GGTCCTGGAC
    AGCATTTATT TCAGCAGGCG CTTCCATGTG CGCTGTGTAG CCCAAGCCAG AGATAAAGCC
    GGACACCTGG GAACTCCACT GAGGAGCAAC ATTGTCACCA TTGGCACTGA GGGATCAATA
    TGCCACACTC CAGTCACCAC AGGGACCGCT CGAGGCTTCC AAGCCCAGTC CTTTATCGCC
    ACTCTAAAAT ACCTGGATGT CAAACACAAA GAGCATCCAA ACAGGATCCA CATTTCAGTT
    CAGATTCCCC ATCAGGATGG GATGCTTCCA CTGGTGTCCA CCATGCCTCT GCATAACCTG
    CACTTCCTGC TCTCTGAGTC CATTTACCGT CAGCAACACG TCTGCTCCAA CCTGGTCACA
    CTTAAAGACC TGCAGGGTAT CTCTGAGACC GGCTTCTTGG ATGACGTGTC ATATGACAGC
    ATATCCCTGG GTCCTGGATA TGACCGGCCA TATCAGTTCA ACCCCAATGT TCGAGAACCC
    AGAACCATAC AGCTGTACAA GCATCTCAAT CTGAAGAGCT GCATCTGGAC ATTCGATGCT
    TATTATGACA TGACGGAGCT TATCGATGTT TGCGGAGGAT CAGTAACTGC CGACTTTCAG
    GTCAGAGACT CGGCTCAGTC CTTCCTGACA GTCCAGGTTC CGCTCTATGT GTCCTACATC
    TACGTGACGG CTCCTCGTGG ATGGGCGTCT CTGGAGCACC ACACAGAAAT GGAGTTCTCC
    TTCTTCTACG ACACTGTCTT ATGGAGAACA GGCATTCAGA CAGACAGCGT GCTTTCGGCC
    AGATTACAGA TCATTCGGAT CTTCATTAAG GATGACGGAC GACTGGTGAT TGAGTTTAAA
    ACCCACGCCA AGTTCAGAGG TCAGTTTGTC CCAGAGCATC ACACCCTCCC CGGACAGAAG
    TCTCATCTGA TGGCTCCAGA TCACTTAGGA GGCATGGAGT TCGACCTACA GCTGGTGTGG
    AGCGCACAGT CTTTTGATTC ACCCTATCAG CTCTGGAGAG CCACAAGCTC CTACAGCAGG
    AAAGACTATT CAGGGGAGTA CACCGTCTTC CTCATTCCCT GCACGGTACA GCCAACACAG
    GCATGGGTGG ACTCTGGGGA CAAGCCTCTA TCCTGCACAG CACATGCACC AGAGAAATTC
    CTGCTGCCCA TCGCCTTCCA GCAGACCAAC CGCCCTGTCC CTGTTGTCTA CTCTCTGAAT
    ACTGAGTTCC AGCTTTGTAA TAATGAGAAA GTTTTTCTAA TGGATCCTGC TCTCGCAGAC
    ATGTCTGTGG CTGAGATGGA TTACAAAGGA GCCTTTTCAA TGGGTCAAAC CCTGTATGGC
    AGAGTCCTGT GGAATCCTGA CCAGAACCTG AATTCAGCCT ACAAGCTGCA GCTGGAGAAA
    GTGTATTTGT GTACGGGACG GGATGGATAT GTTCCCTTCT TTGACCCTAC AGGCACACTG
    TATAATGAGG GCCCTCAGTA TGGCTGCATT CAACCAAACA AGCACCTTAA ACACAGGTTC
    TTACTTCTGG ACCGAAAGCA GCCTGATGTA TGTGATCGCT ACTTCCATGA TGTTCCCTTT
    GAGGCCAGCT TTGCATCTGA CATTCCTGAC CTCCTGTCTT TGTCTGCGAT GCCCGGTGTA
    GACGGATTCA CAATGAAGGT TGATGCCCTT TATAAGGTTG AAGCAGGGCA CCAATGGTAC
    CTCCAGGTCA TCTACGTTAT TGGACCCGAG TCTATTTCGT CTCCCAGAAT CCAGCGCTCA
    GTGACTTATG AGCTGAAGCG AAGCCGCCGA GATCTAGTAG ACCGATCAGG TCGCCTAATG
    CTTGATGAAT CATTGATCTA CGACAATGAA GGTGACCAGG TAAAGAATGG CACCAACATG
    AAGACCTTGA AGCTTGAGAT GGAACCATCA GGCACCTTCA ACCCCCAAAT GGGAGGATCC
    ATTGGGGGTG GCGTGGCAGC CATCCTCCTG CTTTTCTTAG TCCTTCTAGC GTTCTGTTTC
    ATGGTCAGGA AGTGCCGCAG GGTTGTCAAA AAGAGGCCTT CAAAGACGGT GGAGGATTAT
    CCTCTGAACA CAAAGGTGGA GGTCTGCATT GAACGTCTGG AGAAAAACCT CAACAGCAAG
    CACTGCTCTG TAAGAAACAT CAACGTTCTC AACAAGAACC CCGACACCTA CAAAGTCAAT
    GGGGTGAAAG TCAAACAGGT CAATCTGGAG GTCAAACTCC ACAACAATCT AAATGACGGC
    ACTGAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165392.1
    CDS93..12104
    Translation

    Target ORF information:

    RefSeq Version XM_005165335.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165335.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGC AGTGGTTGGT GAATCTCGCG TGATCAGCAC TGATGTCCCC
    TTCACTATTG AAGACCTCAA TTCAGACCAT ATCTTCTACG TGCAAAACAT TCGCCAATCC
    AATATTCGAC AGGATGTCTT CAGCTTTTAT ATCAGTGATG GATCAAGTCA AACTGAGGCT
    TTTGATGTCA CTATAGATAT TCAGCACACC AAAGAGGACC TAGTTCCAAT TATCTCTGTA
    AATAGCATCC ACGTGGAGGA GAACTCTGGT GTGGTCATTA CCAACTCCTC CCTTAACGTA
    CTGGACCGGG ACACGCCAGA GAATGAGCTT TTGCTTACCA TCATCAAGAA GCCTTCATAC
    GGGAAGATAC GCAGAAGGCA GTTTTACTCA GACCCTTTAG AGAATGGCAG GATTCTTCAG
    CAGGGATCCA CCTTCACCTA CCAGGACATT CTTGATGAGC TGCTGGTCTA CACTCCAGAC
    GGACGTGGCT CTGGGACTGA TGAGATCCAG TTTTCAGTAA CAGACGGCAT ATACACGGAG
    ACGGGCCGTC TGGAGTTCAG TATGGATATT AGGAAGAAAG AAGGCCCTAG AGTAACAATC
    AACAGAGGAC TGCAGCTCGC AGCAGGTTCT TCATCTAAAA TCACGGAGCA GCATCTGAAA
    GGAACGGACA TAGATTCAGA TAACCTAAAG CTGAGGTTCA CCCTCATCAA AGACCTAAGT
    GTGGGCTCCC TGATGCTGAA AACAGGCAGA AACAAGGCTC GCGTATCCGT GAAAGGACCC
    GTCACCAGCT TCACCCAGGA TGACATCAAC AAAGGACATG TGGAATACAT TCATGAAAAA
    GGAGAGAGCG GAGGAAGCTT CTCTTTTAAG TTCAACCTAG AAGATCCTGA GGGAAACAGA
    GTCATTGACC AGTCTTTCTT CATTAGTGTT CTGGAGGACA GACTTCCTCC CACAGTGGTG
    GTTAATAAGG GTTTAGTTTT GGATGAGAAC TCCTTGAAGA AGATCACAAC TCTGCAGCTC
    TCCTCTACTG ATCAGGATAG TGAACCCGAT GAACTCGTTT ACCGAATCAC TAAACAGCCC
    CAGCTTGGTC ATCTAGAACA TTCTAACAAA CCAGGAACAC GGATCAGCTC GTTTACCCAG
    GCTGACCTGG CCTCTCGCAG CATTCAGTAT GTGCACACCA GTGAAGACGA GATTCACTCC
    GATGACTTCA CCTTCACTGT ATCTGATGGA GGAAACGAGG TCTCACAGAC GTTCTACATC
    ACCATCCGGC CGGTGGACGA TGCTCTGCCT GTGTTGCAGG TGCCCGGCAT GAGGGTGCAG
    GAGGGCGTGA GGAAGACCAT CACTGAGTTT GAGCTCAAGG CCACCGATGC TGACACTGAG
    GAGGACTCTG TCACTTTCAC CATTGTTCAG GCACCTCGTC ATGGGACCAT CGAGCGCACC
    AGTAATGGTC AGCATTATCG TCAGACAAAC ACCTTCACTA TGGACGACGT CTACCAGAAC
    CGCATCAGTT ACAACCATGA TGGCAGTAAC TCCCTCAAAG ATCGATTCAC CTTCACTGTC
    GGTGACGGCA CCAACATGTT CTTCATCATC GAGGAGGGTG GAAAAGAGGT TGTCACTGCT
    GCTCCTCAAA AGTTTAAAAT TGACATTTTA CCTGTTGATG ACGGGACCCC TCGTATTGTT
    ACCAACCTGG GCCTGCAGTG GCTGGAGTAC ATGGAAAATA AGGCCACTAA TCTGATCTCC
    AAGAAGGAGC TTTTCACTAT CGATCCAGAC ACAGATGATG GACAGCTGAC TTATGAAATC
    ACTACAGAAG CTAAGCACGG CTACCTGGAG AGTAAACTCA ATCCTGGCAA ACCTATCACC
    TCTTTCACAC AAGCTGACAT AAACCTTGGC CTTATCCGAT ACGTTCTAAG CGAGGACAAT
    GTTCAGGAGA CCATGGACAA CTTCAAGTTC CTTGTAAAGG ACAGCAAACC TAACATCGTC
    AGTGACAATG TGTTCCACAT CCAGTGGTCT CTGATCAGCT TTGAACACAA GAGTTTTAAC
    GTGAGTGAGA AGGATGGAAC GGTGGCGGTC ACAGTGAAGA GGACTGGTAA TCTGAACCAG
    TATGCTATTG TGCTGTGTCG AACTGAGCAG GGCACTGCCA CCTCCACCTC CAGCATGGGC
    TCTCGGGCCG GACAGCAGGA TTATGTTGAA TACGCCGGAC AGGTTCAGTT TGATGAGAGA
    GAAGACACCA AGGTGTGCAC CATTGTGATC AACGATGACC AGGTGTTTGA GAACATCGAG
    AGCTTTAATG TGGAGCTGAG TATGCCGGTG TATGCCCTCC TGGGCTCAGT GACCCGAGCT
    AAAGTCAATA TCAATGACAC GGAGGATGAG CCCACTTTGC AGTTTGATAA GAAAACCTAC
    CACGTCAATG AGAGCACTGG TTTCATATTT GCTCCTGTTG AGAGAAAAGG TGACACCAGC
    AGCACAGTTT CTGCCCTGTG CTACACTGTG CCTAAATCAG CTCGGGGCAG CAGTCTTCAC
    GCTCTCGAGT CCGGCTCAGA CTACAAGACC CGTGGCATGA GTGGCGACAA CAGACTCATC
    TTTGGTCCAG GAGTGAGCAT GTCCACCTGT GACGTCAAGC TGATCGATGA CAGCGAGTAT
    GAACTCTCTG AGGAGTTTGA GCTGGTGCTG TCAGACGCCT CAGATAATGC AAGGATGGGC
    GACGTCACTG TGGCCAAAGT CATCATCGAC GGGCCCAACG ACGCCTCTAC TGTGTTTCTG
    GGAAACGCAT CATACACCTT CAGTGAAGAT GCAGGCACCA TTGAAATCCC TGTTCTGCGG
    CAGGGCAGTG ATCTTTCATC AGTCACCTCA GTGTGGTGCG CCACACGTCC TGCAGAACAA
    GATTCAGCCA CGCCTGGAGT CGACTACATC CCCAGCTCCA AAAAAGTAGA GTTCAAACCA
    GGCAAGACCG AGGAGACATG CAGTTTGACC ATAATGGATG ACATCCAGAA CCCAACAATA
    GAAGGGAAAG AATCCTTCAT TGTGTTTCTT AGCTCTGCTA ATGGAGCGGT CCTGACTGAA
    CCCTATGAAG CCTCTGTCTA CATCACTGAC ACCACTCAAG ACATCCCAAG CATGCAGTTT
    GACAAGCTGG CATATACAGT AAAGGAAAAA GATGGCGTCC TGCACATCCC TATTTTTAGG
    ACAGGAGATC TGTCCTATAA GTCTTCTGTC CGCTGTTTCA CCCGCACAAT GTCTGCAATG
    GTTATGGATG ACTTTGAGGA GAGGAGAAAT GCAGATGATT CGAGAATCAC CTTTCAGAAA
    GGGGAGAAGG TGAAGAATTG TACAGTACTG ATCCAGGATG ACTCCGTGTT TGAGCCTGAA
    GAGGAGTTCC AGGTCCACCT TGGAAGCCCA CAGGGTGACC ATTGGATTGG AGCCATGATT
    GGAGTCAACG ACATTGTGAC TGTGACCATC ACAAATGATG AAGATGCACC TACGATCGAG
    TTTGAACAAG CCACATATCA AGTTCGTGAG CCACCTGGTC CCGATGGCAT TGAGGTTTTG
    AATATCAAGG TAATTCGTAA AGGAGACCTC GACCGCACCT CCAAAATTCG ATGCAGCACG
    AGGGATGGAT CGGCCCAGTC AGGTGTGGAC TATAACCCAA AGAGTCGAGT GCTGAAGTAC
    AGCCCTGGTG TGGACCACAT CATGTTTAAG GTGGAGATTC TGTCCAATGA GGACAGAGAG
    TGGCATGAGT CTTTCTCTTT GGTCCTTGGT CCTGACGACC CAGTTGAAGC TGTTCTTGGA
    GAGATCATCA TTGCAACAGT CACTATTCTG GATCAGGAGG CTGCAGGAAG TCTTATCCTT
    CCGGCTCCAC CCATTGTGGT GTCTCTATCA GATTATGACT ACGTTCAGGA GGTCACTAAA
    GAGGGCAGCA AGAAGTCTCC GTCTCCTGGA TATCCGCTGG TCTGTGTGAC CCCCTGTGAC
    CCCCACTACC CCAAATACTC CGTCATGAAG GAGCGCTGTG AGGAGGCAGG CATCAACCAG
    ACTTCTCTAC ACTTCAGCTG GGAGGTGGCA ACTCCTACAG ACACCAGCGG AGCAAGATCT
    CCCTTCGAGA CTGTGACCGA CAACACACCC TACACCACAA TCAACCACAT GGTCCTGGAC
    AGCATTTATT TCAGCAGGCG CTTCCATGTG CGCTGTGTAG CCCAAGCCAG AGATAAAGCC
    GGACACCTGG GAACTCCACT GAGGAGCAAC ATTGTCACCA TTGGCACTGA GGGATCAATA
    TGCCACACTC CAGTCACCAC AGGGACCGCT CGAGGCTTCC AAGCCCAGTC CTTTATCGCC
    ACTCTAAAAT ACCTGGATGT CAAACACAAA GAGCATCCAA ACAGGATCCA CATTTCAGTT
    CAGATTCCCC ATCAGGATGG GATGCTTCCA CTGGTGTCCA CCATGCCTCT GCATAACCTG
    CACTTCCTGC TCTCTGAGTC CATTTACCGT CAGCAACACG TCTGCTCCAA CCTGGTCACA
    CTTAAAGACC TGCAGGGTAT CTCTGAGACC GGCTTCTTGG ATGACGTGTC ATATGACAGC
    ATATCCCTGG GTCCTGGATA TGACCGGCCA TATCAGTTCA ACCCCAATGT TCGAGAACCC
    AGAACCATAC AGCTGTACAA GCATCTCAAT CTGAAGAGCT GCATCTGGAC ATTCGATGCT
    TATTATGACA TGACGGAGCT TATCGATGTT TGCGGAGGAT CAGTAACTGC CGACTTTCAG
    GTCAGAGACT CGGCTCAGTC CTTCCTGACA GTCCAGGTTC CGCTCTATGT GTCCTACATC
    TACGTGACGG CTCCTCGTGG ATGGGCGTCT CTGGAGCACC ACACAGAAAT GGAGTTCTCC
    TTCTTCTACG ACACTGTCTT ATGGAGAACA GGCATTCAGA CAGACAGCGT GCTTTCGGCC
    AGATTACAGA TCATTCGGAT CTTCATTAAG GATGACGGAC GACTGGTGAT TGAGTTTAAA
    ACCCACGCCA AGTTCAGAGG TCAGTTTGTC CCAGAGCATC ACACCCTCCC CGGACAGAAG
    TCTCATCTGA TGGCTCCAGA TCACTTAGGA GGCATGGAGT TCGACCTACA GCTGGTGTGG
    AGCGCACAGT CTTTTGATTC ACCCTATCAG CTCTGGAGAG CCACAAGCTC CTACAGCAGG
    AAAGACTATT CAGGGGAGTA CACCGTCTTC CTCATTCCCT GCACGGTACA GCCAACACAG
    GCATGGGTGG ACTCTGGGGA CAAGCCTCTA TCCTGCACAG CACATGCACC AGAGAAATTC
    CTGCTGCCCA TCGCCTTCCA GCAGACCAAC CGCCCTGTCC CTGTTGTCTA CTCTCTGAAT
    ACTGAGTTCC AGCTTTGTAA TAATGAGAAA GTTTTTCTAA TGGATCCTGC TCTCGCAGAC
    ATGTCTGTGG CTGAGATGGA TTACAAAGGA GCCTTTTCAA TGGGTCAAAC CCTGTATGGC
    AGAGTCCTGT GGAATCCTGA CCAGAACCTG AATTCAGCCT ACAAGCTGCA GCTGGAGAAA
    GTGTATTTGT GTACGGGACG GGATGGATAT GTTCCCTTCT TTGACCCTAC AGGCACACTG
    TATAATGAGG GCCCTCAGTA TGGCTGCATT CAACCAAACA AGCACCTTAA ACACAGGTTC
    TTACTTCTGG ACCGAAAGCA GCCTGATGTA TGTGATCGCT ACTTCCATGA TGTTCCCTTT
    GAGGCCAGCT TTGCATCTGA CATTCCTGAC CTCCTGTCTT TGTCTGCGAT GCCCGGTGTA
    GACGGATTCA CAATGAAGGT TGATGCCCTT TATAAGGTTG AAGCAGGGCA CCAATGGTAC
    CTCCAGGTCA TCTACGTTAT TGGACCCGAG TCTATTTCGT CTCCCAGAAT CCAGCGCTCA
    GTGACTTATG AGCTGAAGCG AAGCCGCCGA GATCTAGTAG ACCGATCAGG TCGCCTAATG
    CTTGATGAAT CATTGATCTA CGACAATGAA GGTGACCAGG TAAAGAATGG CACCAACATG
    AAGACCTTGA AGCTTGAGAT GGAACCATCA GGCACCTTCA ACCCCCAAAT GGGAGGATCC
    ATTGGGGGTG GCGTGGCAGC CATCCTCCTG CTTTTCTTAG TCCTTCTAGC GTTCTGTTTC
    ATGGTCAGGA AGTGCCGCAG GGTTGTCAAA AAGAGGCCTT CAAAGACGGT GGAGGATTAT
    CCTCTGAACA CAAAGGTGGA GGTCTGCATT GAACGTCTGG AGAAAAACCT CAACAGCAAG
    CACTGCTCTG TAAGAAACAT CAACGTTCTC AACAAGAACC CCGACACCTA CAAAGTCAAT
    GGGGTGAAAG TCAAACAGGT CAATCTGGAG GTCAAACTCC ACAACAATCT AAATGACGGC
    ACTGAAGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142680
    Clone ID Related Accession (Same CDS sequence) XM_021476261.1
    Accession Version XM_021476261.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product extracellular matrix protein FRAS1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6901
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    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
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    8521
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    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGT GTCTGGTGGA
    GAACACACCA CCCCAGAGAT GGACTTTGCC ATCCTGCTTT TGGTCAATCA TCAGCAGCCA
    CCAGTCTTTC AGATCCTGGA CCCAGTGCTG GAGGTCAGCC TTGGCGGCAG GGCACCAGTG
    GGTGTGCAAC AGTTGGCAGT AACAGATTCA GACACGGCCC CTGAAGAGCT GGAGTTTGAG
    GTGGTGGAGG GACCACTACA TGGGACGCTC CTGAAAGTGG ACCTTGGATC CCAGGTCCAG
    ATGGTCAATG GGGACACCTT TACCTCCAGT GATGTCACCA GAAACATCCT GCAGTATGAA
    CATGCAGGGC TCACCACTGA AGAGGACTCC ATGACCTTCT CAGTGACTGA CGGGATCTCT
    ATGACCTCCA TCGCGGTGCA GGTTTTGGTG TCAGAGGTCA AAGGCGACGC ACCCAAGCGT
    GACCCAAAGG CTTTTCTTTC CATGGAAGTT GCAGAGAAGA GCAGCAGCAT CATTAGACGT
    TCACATTTAG CTTATATGGA TGATGCATCT CCAGATGAGA GGATTTTTGT GCAGTTAGTC
    TCTGTGCCAA TGTATGGGAT CCTTACAAAA AATAAAGCTG GAGCAGACTA TGAGGAGCTT
    AGTGAATATT CTTCCTTTAC AATGGAGGAC ATCAACAACC ACAAAATAAG GTATGTTACG
    TCCTTTGAAA CTGGGAACCA GCCTGTGACT GACATTTTCC ACTTCGTCGT CTATGATGGT
    GATAACAATC GTCTGGAAAA CCAGATGTTC ACTATCACTA TCACCACTAC CCAGAGGCAG
    CCACCTGTTG TCAGCGTCCG CAGTGGCATT AAAGTTCAGG AAGGTGGGAG AGTCCAGCTC
    TCAACCAATC ACATTATTGC ATCTGACCCA GACACACCCA GAAAGGACCT GCTGGTTTGG
    CTGATCAGAC CACCTAAATT TGGCTTCATT GAGAACACAA AGCAAGCAGT GGTTGGTGAA
    TCTCGCGTGA TCAGCACTGA TGTCCCCTTC ACTATTGAAG ACCTCAATTC AGACCATATC
    TTCTACGTGC AAAACATTCG CCAATCCAAT ATTCGACAGG ATGTCTTCAG CTTTTATATC
    AGTGATGGAT CAAGTCAAAC TGAGGCTTTT GATGTCACTA TAGATATTCA GCACACCAAA
    GAGGACCTAG TTCCAATTAT CTCTGTAAAT AGCATCCACG TGGAGGAGAA CTCTGGTGTG
    GTCATTACCA ACTCCTCCCT TAACGTACTG GACCGGGACA CGCCAGAGAA TGAGCTTTTG
    CTTACCATCA TCAAGAAGCC TTCATACGGG AAGATACGCA GAAGGCAGTT TTACTCAGAC
    CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG GGATCCACCT TCACCTACCA GGACATTCTT
    GATGAGCTGC TGGTCTACAC TCCAGACGGA CGTGGCTCTG GGACTGATGA GATCCAGTTT
    TCAGTAACAG ACGGCATATA CACGGAGACG GGCCGTCTGG AGTTCAGTAT GGATATTAGG
    AAGAAAGAAG GCCCTAGAGT AACAATCAAC AGAGGACTGC AGCTCGCAGC AGGTTCTTCA
    TCTAAAATCA CGGAGCAGCA TCTGAAAGGA ACGGACATAG ATTCAGATAA CCTAAAGCTG
    AGGTTCACCC TCATCAAAGA CCTAAGTGTG GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC
    AAGGCTCGCG TATCCGTGAA AGGACCCGTC ACCAGCTTCA CCCAGGATGA CATCAACAAA
    GGACATGTGG AATACATTCA TGAAAAAGGA GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC
    AACCTAGAAG ATCCTGAGGG AAACAGAGTC ATTGACCAGT CTTTCTTCAT TAGTGTTCTG
    GAGGACAGAC TTCCTCCCAC AGTGGTGGTT AATAAGGGTT TAGTTTTGGA TGAGAACTCC
    TTGAAGAAGA TCACAACTCT GCAGCTCTCC TCTACTGATC AGGATAGTGA ACCCGATGAA
    CTCGTTTACC GAATCACTAA ACAGCCCCAG CTTGGTCATC TAGAACATTC TAACAAACCA
    GGAACACGGA TCAGCTCGTT TACCCAGGCT GACCTGGCCT CTCGCAGCAT TCAGTATGTG
    CACACCAGTG AAGACGAGAT TCACTCCGAT GACTTCACCT TCACTGTATC TGATGGAGGA
    AACGAGGTCT CACAGACGTT CTACATCACC ATCCGGCCGG TGGACGATGC TCTGCCTGTG
    TTGCAGGTGC CCGGCATGAG GGTGCAGGAG GGCGTGAGGA AGACCATCAC TGAGTTTGAG
    CTCAAGGCCA CCGATGCTGA CACTGAGGAG GACTCTGTCA CTTTCACCAT TGTTCAGGCA
    CCTCGTCATG GGACCATCGA GCGCACCAGT AATGGTCAGC ATTATCGTCA GACAAACACC
    TTCACTATGG ACGACGTCTA CCAGAACCGC ATCAGTTACA ACCATGATGG CAGTAACTCC
    CTCAAAGATC GATTCACCTT CACTGTCGGT GACGGCACCA ACATGTTCTT CATCATCGAG
    GAGGGTGGAA AAGAGGTTGT CACTGCTGCT CCTCAAAAGT TTAAAATTGA CATTTTACCT
    GTTGATGACG GGACCCCTCG TATTGTTACC AACCTGGGCC TGCAGTGGCT GGAGTACATG
    GAAAATAAGG CCACTAATCT GATCTCCAAG AAGGAGCTTT TCACTATCGA TCCAGACACA
    GATGATGGAC AGCTGACTTA TGAAATCACT ACAGAAGCTA AGCACGGCTA CCTGGAGAGT
    AAACTCAATC CTGGCAAACC TATCACCTCT TTCACACAAG CTGACATAAA CCTTGGCCTT
    ATCCGATACG TTCTAAGCGA GGACAATGTT CAGGAGACCA TGGACAACTT CAAGTTCCTT
    GTAAAGGACA GCAAACCTAA CATCGTCAGT GACAATGTGT TCCACATCCA GTGGTCTCTG
    ATCAGCTTTG AACACAAGAG TTTTAACGTG AGTGAGAAGG ATGGAACGGT GGCGGTCACA
    GTGAAGAGGA CTGGTAATCT GAACCAGTAT GCTATTGTGC TGTGTCGAAC TGAGCAGGGC
    ACTGCCACCT CCACCTCCAG CATGGGCTCT CGGGCCGGAC AGCAGGATTA TGTTGAATAC
    GCCGGACAGG TTCAGTTTGA TGAGAGAGAA GACACCAAGG TGTGCACCAT TGTGATCAAC
    GATGACCAGG TGTTTGAGAA CATCGAGAGC TTTAATGTGG AGCTGAGTAT GCCGGTGTAT
    GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA GTCAATATCA ATGACACGGA GGATGAGCCC
    ACTTTGCAGT TTGATAAGAA AACCTACCAC GTCAATGAGA GCACTGGTTT CATATTTGCT
    CCTGTTGAGA GAAAAGGTGA CACCAGCAGC ACAGTTTCTG CCCTGTGCTA CACTGTGCCT
    AAATCAGCTC GGGGCAGCAG TCTTCACGCT CTCGAGTCCG GCTCAGACTA CAAGACCCGT
    GGCATGAGTG GCGACAACAG ACTCATCTTT GGTCCAGGAG TGAGCATGTC CACCTGTGAC
    GTCAAGCTGA TCGATGACAG CGAGTATGAA CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA
    GACGCCTCAG ATAATGCAAG GATGGGCGAC GTCACTGTGG CCAAAGTCAT CATCGACGGG
    CCCAACGACG CCTCTACTGT GTTTCTGGGA AACGCATCAT ACACCTTCAG TGAAGATGCA
    GGCACCATTG AAATCCCTGT TCTGCGGCAG GGCAGTGATC TTTCATCAGT CACCTCAGTG
    TGGTGCGCCA CACGTCCTGC AGAACAAGAT TCAGCCACGC CTGGAGTCGA CTACATCCCC
    AGCTCCAAAA AAGTAGAGTT CAAACCAGGC AAGACCGAGG AGACATGCAG TTTGACCATA
    ATGGATGACA TCCAGAACCC AACAATAGAA GGGAAAGAAT CCTTCATTGT GTTTCTTAGC
    TCTGCTAATG GAGCGGTCCT GACTGAACCC TATGAAGCCT CTGTCTACAT CACTGACACC
    ACTCAAGACA TCCCAAGCAT GCAGTTTGAC AAGCTGGCAT ATACAGTAAA GGAAAAAGAT
    GGCGTCCTGC ACATCCCTAT TTTTAGGACA GGAGATCTGT CCTATAAGTC TTCTGTCCGC
    TGTTTCACCC GCACAATGTC TGCAATGGTT ATGGATGACT TTGAGGAGAG GAGAAATGCA
    GATGATTCGA GAATCACCTT TCAGAAAGGG GAGAAGGTGA AGAATTGTAC AGTACTGATC
    CAGGATGACT CCGTGTTTGA GCCTGAAGAG GAGTTCCAGG TCCACCTTGG AAGCCCACAG
    GGTGACCATT GGATTGGAGC CATGATTGGA GTCAACGACA TTGTGACTGT GACCATCACA
    AATGATGAAG ATGCACCTAC GATCGAGTTT GAACAAGCCA CATATCAAGT TCGTGAGCCA
    CCTGGTCCCG ATGGCATTGA GGTTTTGAAT ATCAAGGTAA TTCGTAAAGG AGACCTCGAC
    CGCACCTCCA AAATTCGATG CAGCACGAGG GATGGATCGG CCCAGTCAGG TGTGGACTAT
    AACCCAAAGA GTCGAGTGCT GAAGTACAGC CCTGGTGTGG ACCACATCAT GTTTAAGGTG
    GAGATTCTGT CCAATGAGGA CAGAGAGTGG CATGAGTCTT TCTCTTTGGT CCTTGGTCCT
    GACGACCCAG TTGAAGCTGT TCTTGGAGAG ATCATCATTG CAACAGTCAC TATTCTGGAT
    CAGGAGGCTG CAGGAAGTCT TATCCTTCCG GCTCCACCCA TTGTGGTGTC TCTATCAGAT
    TATGACTACG TTCAGGAGGT CACTAAAGAG GGCAGCAAGA AGTCTCCGTC TCCTGGATAT
    CCGCTGGTCT GTGTGACCCC CTGTGACCCC CACTACCCCA AATACTCCGT CATGAAGGAG
    CGCTGTGAGG AGGCAGGCAT CAACCAGACT TCTCTACACT TCAGCTGGGA GGTGGCAACT
    CCTACAGACA CCAGCGGAGC AAGATCTCCC TTCGAGACTG TGACCGACAA CACACCCTAC
    ACCACAATCA ACCACATGGT CCTGGACAGC ATTTATTTCA GCAGGCGCTT CCATGTGCGC
    TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA CACCTGGGAA CTCCACTGAG GAGCAACATT
    GTCACCATTG GCACTGAGGG ATCAATATGC CACACTCCAG TCACCACAGG GACCGCTCGA
    GGCTTCCAAG CCCAGTCCTT TATCGCCACT CTAAAATACC TGGATGTCAA ACACAAAGAG
    CATCCAAACA GGATCCACAT TTCAGTTCAG ATTCCCCATC AGGATGGGAT GCTTCCACTG
    GTGTCCACCA TGCCTCTGCA TAACCTGCAC TTCCTGCTCT CTGAGTCCAT TTACCGTCAG
    CAACACGTCT GCTCCAACCT GGTCACACTT AAAGACCTGC AGGGTATCTC TGAGACCGGC
    TTCTTGGATG ACGTGTCATA TGACAGCATA TCCCTGGGTC CTGGATATGA CCGGCCATAT
    CAGTTCAACC CCAATGTTCG AGAACCCAGA ACCATACAGC TGTACAAGCA TCTCAATCTG
    AAGAGCTGCA TCTGGACATT CGATGCTTAT TATGACATGA CGGAGCTTAT CGATGTTTGC
    GGAGGATCAG TAACTGCCGA CTTTCAGGTC AGAGACTCGG CTCAGTCCTT CCTGACAGTC
    CAGGTTCCGC TCTATGTGTC CTACATCTAC GTGACGGCTC CTCGTGGATG GGCGTCTCTG
    GAGCACCACA CAGAAATGGA GTTCTCCTTC TTCTACGACA CTGTCTTATG GAGAACAGGC
    ATTCAGACAG ACAGCGTGCT TTCGGCCAGA TTACAGATCA TTCGGATCTT CATTAAGGAT
    GACGGACGAC TGGTGATTGA GTTTAAAACC CACGCCAAGT TCAGAGGTCA GTTTGTCCCA
    GAGCATCACA CCCTCCCCGG ACAGAAGTCT CATCTGATGG CTCCAGATCA CTTAGGAGGC
    ATGGAGTTCG ACCTACAGCT GGTGTGGAGC GCACAGTCTT TTGATTCACC CTATCAGCTC
    TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA GACTATTCAG GGGAGTACAC CGTCTTCCTC
    ATTCCCTGCA CGGTACAGCC AACACAGGCA TGGGTGGACT CTGGGGACAA GCCTCTATCC
    TGCACAGCAC ATGCACCAGA GAAATTCCTG CTGCCCATCG CCTTCCAGCA GACCAACCGC
    CCTGTCCCTG TTGTCTACTC TCTGAATACT GAGTTCCAGC TTTGTAATAA TGAGAAAGTT
    TTTCTAATGG ATCCTGCTCT CGCAGACATG TCTGTGGCTG AGATGGATTA CAAAGGAGCC
    TTTTCAATGG GTCAAACCCT GTATGGCAGA GTCCTGTGGA ATCCTGACCA GAACCTGAAT
    TCAGCCTACA AGCTGCAGCT GGAGAAAGTG TATTTGTGTA CGGGACGGGA TGGATATGTT
    CCCTTCTTTG ACCCTACAGG CACACTGTAT AATGAGGGCC CTCAGTATGG CTGCATTCAA
    CCAAACAAGC ACCTTAAACA CAGGTTCTTA CTTCTGGACC GAAAGCAGCC TGATGTATGT
    GATCGCTACT TCCATGATGT TCCCTTTGAG GCCAGCTTTG CATCTGACAT TCCTGACCTC
    CTGTCTTTGT CTGCGATGCC CGGTGTAGAC GGATTCACAA TGAAGGTTGA TGCCCTTTAT
    AAGGTTGAAG CAGGGCACCA ATGGTACCTC CAGGTCATCT ACGTTATTGG ACCCGAGTCT
    ATTTCGTCTC CCAGAATCCA GCGCTCAGTG ACTTATGAGC TGAAGCGAAG CCGCCGAGAT
    CTAGTAGACC GATCAGGTCG CCTAATGCTT GATGAATCAT TGATCTACGA CAATGAAGGT
    GACCAGGTAA AGAATGGCAC CAACATGAAG ACCTTGAAGC TTGAGATGGA ACCATCAGGC
    ACCTTCAACC CCCAAATGGG AGGATCCATT GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT
    TTCTTAGTCC TTCTAGCGTT CTGTTTCATG GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG
    AGGCCTTCAA AGACGGTGGA GGATTATCCT CTGAACACAA AGGTGGAGGT CTGCATTGAA
    CGTCTGGAGA AAAACCTCAA CAGCAAGCAC TGCTCTGTAA GAAACATCAA CGTTCTCAAC
    AAGAACCCCG ACACCTACAA AGTCAATGGG GTGAAAGTCA AACAGGTCAA TCTGGAGGTC
    AAACTCCACA ACAATCTAAA TGACGGCACT GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331936.1
    CDS358..12336
    Translation

    Target ORF information:

    RefSeq Version XM_021476261.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476261.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGT GTCTGGTGGA
    GAACACACCA CCCCAGAGAT GGACTTTGCC ATCCTGCTTT TGGTCAATCA TCAGCAGCCA
    CCAGTCTTTC AGATCCTGGA CCCAGTGCTG GAGGTCAGCC TTGGCGGCAG GGCACCAGTG
    GGTGTGCAAC AGTTGGCAGT AACAGATTCA GACACGGCCC CTGAAGAGCT GGAGTTTGAG
    GTGGTGGAGG GACCACTACA TGGGACGCTC CTGAAAGTGG ACCTTGGATC CCAGGTCCAG
    ATGGTCAATG GGGACACCTT TACCTCCAGT GATGTCACCA GAAACATCCT GCAGTATGAA
    CATGCAGGGC TCACCACTGA AGAGGACTCC ATGACCTTCT CAGTGACTGA CGGGATCTCT
    ATGACCTCCA TCGCGGTGCA GGTTTTGGTG TCAGAGGTCA AAGGCGACGC ACCCAAGCGT
    GACCCAAAGG CTTTTCTTTC CATGGAAGTT GCAGAGAAGA GCAGCAGCAT CATTAGACGT
    TCACATTTAG CTTATATGGA TGATGCATCT CCAGATGAGA GGATTTTTGT GCAGTTAGTC
    TCTGTGCCAA TGTATGGGAT CCTTACAAAA AATAAAGCTG GAGCAGACTA TGAGGAGCTT
    AGTGAATATT CTTCCTTTAC AATGGAGGAC ATCAACAACC ACAAAATAAG GTATGTTACG
    TCCTTTGAAA CTGGGAACCA GCCTGTGACT GACATTTTCC ACTTCGTCGT CTATGATGGT
    GATAACAATC GTCTGGAAAA CCAGATGTTC ACTATCACTA TCACCACTAC CCAGAGGCAG
    CCACCTGTTG TCAGCGTCCG CAGTGGCATT AAAGTTCAGG AAGGTGGGAG AGTCCAGCTC
    TCAACCAATC ACATTATTGC ATCTGACCCA GACACACCCA GAAAGGACCT GCTGGTTTGG
    CTGATCAGAC CACCTAAATT TGGCTTCATT GAGAACACAA AGCAAGCAGT GGTTGGTGAA
    TCTCGCGTGA TCAGCACTGA TGTCCCCTTC ACTATTGAAG ACCTCAATTC AGACCATATC
    TTCTACGTGC AAAACATTCG CCAATCCAAT ATTCGACAGG ATGTCTTCAG CTTTTATATC
    AGTGATGGAT CAAGTCAAAC TGAGGCTTTT GATGTCACTA TAGATATTCA GCACACCAAA
    GAGGACCTAG TTCCAATTAT CTCTGTAAAT AGCATCCACG TGGAGGAGAA CTCTGGTGTG
    GTCATTACCA ACTCCTCCCT TAACGTACTG GACCGGGACA CGCCAGAGAA TGAGCTTTTG
    CTTACCATCA TCAAGAAGCC TTCATACGGG AAGATACGCA GAAGGCAGTT TTACTCAGAC
    CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG GGATCCACCT TCACCTACCA GGACATTCTT
    GATGAGCTGC TGGTCTACAC TCCAGACGGA CGTGGCTCTG GGACTGATGA GATCCAGTTT
    TCAGTAACAG ACGGCATATA CACGGAGACG GGCCGTCTGG AGTTCAGTAT GGATATTAGG
    AAGAAAGAAG GCCCTAGAGT AACAATCAAC AGAGGACTGC AGCTCGCAGC AGGTTCTTCA
    TCTAAAATCA CGGAGCAGCA TCTGAAAGGA ACGGACATAG ATTCAGATAA CCTAAAGCTG
    AGGTTCACCC TCATCAAAGA CCTAAGTGTG GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC
    AAGGCTCGCG TATCCGTGAA AGGACCCGTC ACCAGCTTCA CCCAGGATGA CATCAACAAA
    GGACATGTGG AATACATTCA TGAAAAAGGA GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC
    AACCTAGAAG ATCCTGAGGG AAACAGAGTC ATTGACCAGT CTTTCTTCAT TAGTGTTCTG
    GAGGACAGAC TTCCTCCCAC AGTGGTGGTT AATAAGGGTT TAGTTTTGGA TGAGAACTCC
    TTGAAGAAGA TCACAACTCT GCAGCTCTCC TCTACTGATC AGGATAGTGA ACCCGATGAA
    CTCGTTTACC GAATCACTAA ACAGCCCCAG CTTGGTCATC TAGAACATTC TAACAAACCA
    GGAACACGGA TCAGCTCGTT TACCCAGGCT GACCTGGCCT CTCGCAGCAT TCAGTATGTG
    CACACCAGTG AAGACGAGAT TCACTCCGAT GACTTCACCT TCACTGTATC TGATGGAGGA
    AACGAGGTCT CACAGACGTT CTACATCACC ATCCGGCCGG TGGACGATGC TCTGCCTGTG
    TTGCAGGTGC CCGGCATGAG GGTGCAGGAG GGCGTGAGGA AGACCATCAC TGAGTTTGAG
    CTCAAGGCCA CCGATGCTGA CACTGAGGAG GACTCTGTCA CTTTCACCAT TGTTCAGGCA
    CCTCGTCATG GGACCATCGA GCGCACCAGT AATGGTCAGC ATTATCGTCA GACAAACACC
    TTCACTATGG ACGACGTCTA CCAGAACCGC ATCAGTTACA ACCATGATGG CAGTAACTCC
    CTCAAAGATC GATTCACCTT CACTGTCGGT GACGGCACCA ACATGTTCTT CATCATCGAG
    GAGGGTGGAA AAGAGGTTGT CACTGCTGCT CCTCAAAAGT TTAAAATTGA CATTTTACCT
    GTTGATGACG GGACCCCTCG TATTGTTACC AACCTGGGCC TGCAGTGGCT GGAGTACATG
    GAAAATAAGG CCACTAATCT GATCTCCAAG AAGGAGCTTT TCACTATCGA TCCAGACACA
    GATGATGGAC AGCTGACTTA TGAAATCACT ACAGAAGCTA AGCACGGCTA CCTGGAGAGT
    AAACTCAATC CTGGCAAACC TATCACCTCT TTCACACAAG CTGACATAAA CCTTGGCCTT
    ATCCGATACG TTCTAAGCGA GGACAATGTT CAGGAGACCA TGGACAACTT CAAGTTCCTT
    GTAAAGGACA GCAAACCTAA CATCGTCAGT GACAATGTGT TCCACATCCA GTGGTCTCTG
    ATCAGCTTTG AACACAAGAG TTTTAACGTG AGTGAGAAGG ATGGAACGGT GGCGGTCACA
    GTGAAGAGGA CTGGTAATCT GAACCAGTAT GCTATTGTGC TGTGTCGAAC TGAGCAGGGC
    ACTGCCACCT CCACCTCCAG CATGGGCTCT CGGGCCGGAC AGCAGGATTA TGTTGAATAC
    GCCGGACAGG TTCAGTTTGA TGAGAGAGAA GACACCAAGG TGTGCACCAT TGTGATCAAC
    GATGACCAGG TGTTTGAGAA CATCGAGAGC TTTAATGTGG AGCTGAGTAT GCCGGTGTAT
    GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA GTCAATATCA ATGACACGGA GGATGAGCCC
    ACTTTGCAGT TTGATAAGAA AACCTACCAC GTCAATGAGA GCACTGGTTT CATATTTGCT
    CCTGTTGAGA GAAAAGGTGA CACCAGCAGC ACAGTTTCTG CCCTGTGCTA CACTGTGCCT
    AAATCAGCTC GGGGCAGCAG TCTTCACGCT CTCGAGTCCG GCTCAGACTA CAAGACCCGT
    GGCATGAGTG GCGACAACAG ACTCATCTTT GGTCCAGGAG TGAGCATGTC CACCTGTGAC
    GTCAAGCTGA TCGATGACAG CGAGTATGAA CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA
    GACGCCTCAG ATAATGCAAG GATGGGCGAC GTCACTGTGG CCAAAGTCAT CATCGACGGG
    CCCAACGACG CCTCTACTGT GTTTCTGGGA AACGCATCAT ACACCTTCAG TGAAGATGCA
    GGCACCATTG AAATCCCTGT TCTGCGGCAG GGCAGTGATC TTTCATCAGT CACCTCAGTG
    TGGTGCGCCA CACGTCCTGC AGAACAAGAT TCAGCCACGC CTGGAGTCGA CTACATCCCC
    AGCTCCAAAA AAGTAGAGTT CAAACCAGGC AAGACCGAGG AGACATGCAG TTTGACCATA
    ATGGATGACA TCCAGAACCC AACAATAGAA GGGAAAGAAT CCTTCATTGT GTTTCTTAGC
    TCTGCTAATG GAGCGGTCCT GACTGAACCC TATGAAGCCT CTGTCTACAT CACTGACACC
    ACTCAAGACA TCCCAAGCAT GCAGTTTGAC AAGCTGGCAT ATACAGTAAA GGAAAAAGAT
    GGCGTCCTGC ACATCCCTAT TTTTAGGACA GGAGATCTGT CCTATAAGTC TTCTGTCCGC
    TGTTTCACCC GCACAATGTC TGCAATGGTT ATGGATGACT TTGAGGAGAG GAGAAATGCA
    GATGATTCGA GAATCACCTT TCAGAAAGGG GAGAAGGTGA AGAATTGTAC AGTACTGATC
    CAGGATGACT CCGTGTTTGA GCCTGAAGAG GAGTTCCAGG TCCACCTTGG AAGCCCACAG
    GGTGACCATT GGATTGGAGC CATGATTGGA GTCAACGACA TTGTGACTGT GACCATCACA
    AATGATGAAG ATGCACCTAC GATCGAGTTT GAACAAGCCA CATATCAAGT TCGTGAGCCA
    CCTGGTCCCG ATGGCATTGA GGTTTTGAAT ATCAAGGTAA TTCGTAAAGG AGACCTCGAC
    CGCACCTCCA AAATTCGATG CAGCACGAGG GATGGATCGG CCCAGTCAGG TGTGGACTAT
    AACCCAAAGA GTCGAGTGCT GAAGTACAGC CCTGGTGTGG ACCACATCAT GTTTAAGGTG
    GAGATTCTGT CCAATGAGGA CAGAGAGTGG CATGAGTCTT TCTCTTTGGT CCTTGGTCCT
    GACGACCCAG TTGAAGCTGT TCTTGGAGAG ATCATCATTG CAACAGTCAC TATTCTGGAT
    CAGGAGGCTG CAGGAAGTCT TATCCTTCCG GCTCCACCCA TTGTGGTGTC TCTATCAGAT
    TATGACTACG TTCAGGAGGT CACTAAAGAG GGCAGCAAGA AGTCTCCGTC TCCTGGATAT
    CCGCTGGTCT GTGTGACCCC CTGTGACCCC CACTACCCCA AATACTCCGT CATGAAGGAG
    CGCTGTGAGG AGGCAGGCAT CAACCAGACT TCTCTACACT TCAGCTGGGA GGTGGCAACT
    CCTACAGACA CCAGCGGAGC AAGATCTCCC TTCGAGACTG TGACCGACAA CACACCCTAC
    ACCACAATCA ACCACATGGT CCTGGACAGC ATTTATTTCA GCAGGCGCTT CCATGTGCGC
    TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA CACCTGGGAA CTCCACTGAG GAGCAACATT
    GTCACCATTG GCACTGAGGG ATCAATATGC CACACTCCAG TCACCACAGG GACCGCTCGA
    GGCTTCCAAG CCCAGTCCTT TATCGCCACT CTAAAATACC TGGATGTCAA ACACAAAGAG
    CATCCAAACA GGATCCACAT TTCAGTTCAG ATTCCCCATC AGGATGGGAT GCTTCCACTG
    GTGTCCACCA TGCCTCTGCA TAACCTGCAC TTCCTGCTCT CTGAGTCCAT TTACCGTCAG
    CAACACGTCT GCTCCAACCT GGTCACACTT AAAGACCTGC AGGGTATCTC TGAGACCGGC
    TTCTTGGATG ACGTGTCATA TGACAGCATA TCCCTGGGTC CTGGATATGA CCGGCCATAT
    CAGTTCAACC CCAATGTTCG AGAACCCAGA ACCATACAGC TGTACAAGCA TCTCAATCTG
    AAGAGCTGCA TCTGGACATT CGATGCTTAT TATGACATGA CGGAGCTTAT CGATGTTTGC
    GGAGGATCAG TAACTGCCGA CTTTCAGGTC AGAGACTCGG CTCAGTCCTT CCTGACAGTC
    CAGGTTCCGC TCTATGTGTC CTACATCTAC GTGACGGCTC CTCGTGGATG GGCGTCTCTG
    GAGCACCACA CAGAAATGGA GTTCTCCTTC TTCTACGACA CTGTCTTATG GAGAACAGGC
    ATTCAGACAG ACAGCGTGCT TTCGGCCAGA TTACAGATCA TTCGGATCTT CATTAAGGAT
    GACGGACGAC TGGTGATTGA GTTTAAAACC CACGCCAAGT TCAGAGGTCA GTTTGTCCCA
    GAGCATCACA CCCTCCCCGG ACAGAAGTCT CATCTGATGG CTCCAGATCA CTTAGGAGGC
    ATGGAGTTCG ACCTACAGCT GGTGTGGAGC GCACAGTCTT TTGATTCACC CTATCAGCTC
    TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA GACTATTCAG GGGAGTACAC CGTCTTCCTC
    ATTCCCTGCA CGGTACAGCC AACACAGGCA TGGGTGGACT CTGGGGACAA GCCTCTATCC
    TGCACAGCAC ATGCACCAGA GAAATTCCTG CTGCCCATCG CCTTCCAGCA GACCAACCGC
    CCTGTCCCTG TTGTCTACTC TCTGAATACT GAGTTCCAGC TTTGTAATAA TGAGAAAGTT
    TTTCTAATGG ATCCTGCTCT CGCAGACATG TCTGTGGCTG AGATGGATTA CAAAGGAGCC
    TTTTCAATGG GTCAAACCCT GTATGGCAGA GTCCTGTGGA ATCCTGACCA GAACCTGAAT
    TCAGCCTACA AGCTGCAGCT GGAGAAAGTG TATTTGTGTA CGGGACGGGA TGGATATGTT
    CCCTTCTTTG ACCCTACAGG CACACTGTAT AATGAGGGCC CTCAGTATGG CTGCATTCAA
    CCAAACAAGC ACCTTAAACA CAGGTTCTTA CTTCTGGACC GAAAGCAGCC TGATGTATGT
    GATCGCTACT TCCATGATGT TCCCTTTGAG GCCAGCTTTG CATCTGACAT TCCTGACCTC
    CTGTCTTTGT CTGCGATGCC CGGTGTAGAC GGATTCACAA TGAAGGTTGA TGCCCTTTAT
    AAGGTTGAAG CAGGGCACCA ATGGTACCTC CAGGTCATCT ACGTTATTGG ACCCGAGTCT
    ATTTCGTCTC CCAGAATCCA GCGCTCAGTG ACTTATGAGC TGAAGCGAAG CCGCCGAGAT
    CTAGTAGACC GATCAGGTCG CCTAATGCTT GATGAATCAT TGATCTACGA CAATGAAGGT
    GACCAGGTAA AGAATGGCAC CAACATGAAG ACCTTGAAGC TTGAGATGGA ACCATCAGGC
    ACCTTCAACC CCCAAATGGG AGGATCCATT GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT
    TTCTTAGTCC TTCTAGCGTT CTGTTTCATG GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG
    AGGCCTTCAA AGACGGTGGA GGATTATCCT CTGAACACAA AGGTGGAGGT CTGCATTGAA
    CGTCTGGAGA AAAACCTCAA CAGCAAGCAC TGCTCTGTAA GAAACATCAA CGTTCTCAAC
    AAGAACCCCG ACACCTACAA AGTCAATGGG GTGAAAGTCA AACAGGTCAA TCTGGAGGTC
    AAACTCCACA ACAATCTAAA TGACGGCACT GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44084
    Clone ID Related Accession (Same CDS sequence) XM_009301656.2 , XM_009301656.3
    Accession Version XM_009301656.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product extracellular matrix protein FRAS1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGT GGTTGGTGAA TCTCGCGTGA TCAGCACTGA TGTCCCCTTC
    ACTATTGAAG ACCTCAATTC AGACCATATC TTCTACGTGC AAAACATTCG CCAATCCAAT
    ATTCGACAGG ATGTCTTCAG CTTTTATATC AGTGATGGAT CAAGTCAAAC TGAGGCTTTT
    GATGTCACTA TAGATATTCA GCACACCAAA GAGGACCTAG TTCCAATTAT CTCTGTAAAT
    AGCATCCACG TGGAGGAGAA CTCTGGTGTG GTCATTACCA ACTCCTCCCT TAACGTACTG
    GACCGGGACA CGCCAGAGAA TGAGCTTTTG CTTACCATCA TCAAGAAGCC TTCATACGGG
    AAGATACGCA GAAGGCAGTT TTACTCAGAC CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG
    GGATCCACCT TCACCTACCA GGACATTCTT GATGAGCTGC TGGTCTACAC TCCAGACGGA
    CGTGGCTCTG GGACTGATGA GATCCAGTTT TCAGTAACAG ACGGCATATA CACGGAGACG
    GGCCGTCTGG AGTTCAGTAT GGATATTAGG AAGAAAGAAG GCCCTAGAGT AACAATCAAC
    AGAGGACTGC AGCTCGCAGC AGGTTCTTCA TCTAAAATCA CGGAGCAGCA TCTGAAAGGA
    ACGGACATAG ATTCAGATAA CCTAAAGCTG AGGTTCACCC TCATCAAAGA CCTAAGTGTG
    GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC AAGGCTCGCG TATCCGTGAA AGGACCCGTC
    ACCAGCTTCA CCCAGGATGA CATCAACAAA GGACATGTGG AATACATTCA TGAAAAAGGA
    GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC AACCTAGAAG ATCCTGAGGG AAACAGAGTC
    ATTGACCAGT CTTTCTTCAT TAGTGTTCTG GAGGACAGAC TTCCTCCCAC AGTGGTGGTT
    AATAAGGGTT TAGTTTTGGA TGAGAACTCC TTGAAGAAGA TCACAACTCT GCAGCTCTCC
    TCTACTGATC AGGATAGTGA ACCCGATGAA CTCGTTTACC GAATCACTAA ACAGCCCCAG
    CTTGGTCATC TAGAACATTC TAACAAACCA GGAACACGGA TCAGCTCGTT TACCCAGGCT
    GACCTGGCCT CTCGCAGCAT TCAGTATGTG CACACCAGTG AAGACGAGAT TCACTCCGAT
    GACTTCACCT TCACTGTATC TGATGGAGGA AACGAGGTCT CACAGACGTT CTACATCACC
    ATCCGGCCGG TGGACGATGC TCTGCCTGTG TTGCAGGTGC CCGGCATGAG GGTGCAGGAG
    GGCGTGAGGA AGACCATCAC TGAGTTTGAG CTCAAGGCCA CCGATGCTGA CACTGAGGAG
    GACTCTGTCA CTTTCACCAT TGTTCAGGCA CCTCGTCATG GGACCATCGA GCGCACCAGT
    AATGGTCAGC ATTATCGTCA GACAAACACC TTCACTATGG ACGACGTCTA CCAGAACCGC
    ATCAGTTACA ACCATGATGG CAGTAACTCC CTCAAAGATC GATTCACCTT CACTGTCGGT
    GACGGCACCA ACATGTTCTT CATCATCGAG GAGGGTGGAA AAGAGGTTGT CACTGCTGCT
    CCTCAAAAGT TTAAAATTGA CATTTTACCT GTTGATGACG GGACCCCTCG TATTGTTACC
    AACCTGGGCC TGCAGTGGCT GGAGTACATG GAAAATAAGG CCACTAATCT GATCTCCAAG
    AAGGAGCTTT TCACTATCGA TCCAGACACA GATGATGGAC AGCTGACTTA TGAAATCACT
    ACAGAAGCTA AGCACGGCTA CCTGGAGAGT AAACTCAATC CTGGCAAACC TATCACCTCT
    TTCACACAAG CTGACATAAA CCTTGGCCTT ATCCGATACG TTCTAAGCGA GGACAATGTT
    CAGGAGACCA TGGACAACTT CAAGTTCCTT GTAAAGGACA GCAAACCTAA CATCGTCAGT
    GACAATGTGT TCCACATCCA GTGGTCTCTG ATCAGCTTTG AACACAAGAG TTTTAACGTG
    AGTGAGAAGG ATGGAACGGT GGCGGTCACA GTGAAGAGGA CTGGTAATCT GAACCAGTAT
    GCTATTGTGC TGTGTCGAAC TGAGCAGGGC ACTGCCACCT CCACCTCCAG CATGGGCTCT
    CGGGCCGGAC AGCAGGATTA TGTTGAATAC GCCGGACAGG TTCAGTTTGA TGAGAGAGAA
    GACACCAAGG TGTGCACCAT TGTGATCAAC GATGACCAGG TGTTTGAGAA CATCGAGAGC
    TTTAATGTGG AGCTGAGTAT GCCGGTGTAT GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA
    GTCAATATCA ATGACACGGA GGATGAGCCC ACTTTGCAGT TTGATAAGAA AACCTACCAC
    GTCAATGAGA GCACTGGTTT CATATTTGCT CCTGTTGAGA GAAAAGGTGA CACCAGCAGC
    ACAGTTTCTG CCCTGTGCTA CACTGTGCCT AAATCAGCTC GGGGCAGCAG TCTTCACGCT
    CTCGAGTCCG GCTCAGACTA CAAGACCCGT GGCATGAGTG GCGACAACAG ACTCATCTTT
    GGTCCAGGAG TGAGCATGTC CACCTGTGAC GTCAAGCTGA TCGATGACAG CGAGTATGAA
    CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA GACGCCTCAG ATAATGCAAG GATGGGCGAC
    GTCACTGTGG CCAAAGTCAT CATCGACGGG CCCAACGACG CCTCTACTGT GTTTCTGGGA
    AACGCATCAT ACACCTTCAG TGAAGATGCA GGCACCATTG AAATCCCTGT TCTGCGGCAG
    GGCAGTGATC TTTCATCAGT CACCTCAGTG TGGTGCGCCA CACGTCCTGC AGAACAAGAT
    TCAGCCACGC CTGGAGTCGA CTACATCCCC AGCTCCAAAA AAGTAGAGTT CAAACCAGGC
    AAGACCGAGG AGACATGCAG TTTGACCATA ATGGATGACA TCCAGAACCC AACAATAGAA
    GGGAAAGAAT CCTTCATTGT GTTTCTTAGC TCTGCTAATG GAGCGGTCCT GACTGAACCC
    TATGAAGCCT CTGTCTACAT CACTGACACC ACTCAAGACA TCCCAAGCAT GCAGTTTGAC
    AAGCTGGCAT ATACAGTAAA GGAAAAAGAT GGCGTCCTGC ACATCCCTAT TTTTAGGACA
    GGAGATCTGT CCTATAAGTC TTCTGTCCGC TGTTTCACCC GCACAATGTC TGCAATGGTT
    ATGGATGACT TTGAGGAGAG GAGAAATGCA GATGATTCGA GAATCACCTT TCAGAAAGGG
    GAGAAGGTGA AGAATTGTAC AGTACTGATC CAGGATGACT CCGTGTTTGA GCCTGAAGAG
    GAGTTCCAGG TCCACCTTGG AAGCCCACAG GGTGACCATT GGATTGGAGC CATGATTGGA
    GTCAACGACA TTGTGACTGT GACCATCACA AATGATGAAG ATGCACCTAC GATCGAGTTT
    GAACAAGCCA CATATCAAGT TCGTGAGCCA CCTGGTCCCG ATGGCATTGA GGTTTTGAAT
    ATCAAGGTAA TTCGTAAAGG AGACCTCGAC CGCACCTCCA AAATTCGATG CAGCACGAGG
    GATGGATCGG CCCAGTCAGG TGTGGACTAT AACCCAAAGA GTCGAGTGCT GAAGTACAGC
    CCTGGTGTGG ACCACATCAT GTTTAAGGTG GAGATTCTGT CCAATGAGGA CAGAGAGTGG
    CATGAGTCTT TCTCTTTGGT CCTTGGTCCT GACGACCCAG TTGAAGCTGT TCTTGGAGAG
    ATCATCATTG CAACAGTCAC TATTCTGGAT CAGGAGGCTG CAGGAAGTCT TATCCTTCCG
    GCTCCACCCA TTGTGGTGTC TCTATCAGAT TATGACTACG TTCAGGAGGT CACTAAAGAG
    GGCAGCAAGA AGTCTCCGTC TCCTGGATAT CCGCTGGTCT GTGTGACCCC CTGTGACCCC
    CACTACCCCA AATACTCCGT CATGAAGGAG CGCTGTGAGG AGGCAGGCAT CAACCAGACT
    TCTCTACACT TCAGCTGGGA GGTGGCAACT CCTACAGACA CCAGCGGAGC AAGATCTCCC
    TTCGAGACTG TGACCGACAA CACACCCTAC ACCACAATCA ACCACATGGT CCTGGACAGC
    ATTTATTTCA GCAGGCGCTT CCATGTGCGC TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA
    CACCTGGGAA CTCCACTGAG GAGCAACATT GTCACCATTG GCACTGAGGG ATCAATATGC
    CACACTCCAG TCACCACAGG GACCGCTCGA GGCTTCCAAG CCCAGTCCTT TATCGCCACT
    CTAAAATACC TGGATGTCAA ACACAAAGAG CATCCAAACA GGATCCACAT TTCAGTTCAG
    ATTCCCCATC AGGATGGGAT GCTTCCACTG GTGTCCACCA TGCCTCTGCA TAACCTGCAC
    TTCCTGCTCT CTGAGTCCAT TTACCGTCAG CAACACGTCT GCTCCAACCT GGTCACACTT
    AAAGACCTGC AGGGTATCTC TGAGACCGGC TTCTTGGATG ACGTGTCATA TGACAGCATA
    TCCCTGGGTC CTGGATATGA CCGGCCATAT CAGTTCAACC CCAATGTTCG AGAACCCAGA
    ACCATACAGC TGTACAAGCA TCTCAATCTG AAGAGCTGCA TCTGGACATT CGATGCTTAT
    TATGACATGA CGGAGCTTAT CGATGTTTGC GGAGGATCAG TAACTGCCGA CTTTCAGGTC
    AGAGACTCGG CTCAGTCCTT CCTGACAGTC CAGGTTCCGC TCTATGTGTC CTACATCTAC
    GTGACGGCTC CTCGTGGATG GGCGTCTCTG GAGCACCACA CAGAAATGGA GTTCTCCTTC
    TTCTACGACA CTGTCTTATG GAGAACAGGC ATTCAGACAG ACAGCGTGCT TTCGGCCAGA
    TTACAGATCA TTCGGATCTT CATTAAGGAT GACGGACGAC TGGTGATTGA GTTTAAAACC
    CACGCCAAGT TCAGAGGTCA GTTTGTCCCA GAGCATCACA CCCTCCCCGG ACAGAAGTCT
    CATCTGATGG CTCCAGATCA CTTAGGAGGC ATGGAGTTCG ACCTACAGCT GGTGTGGAGC
    GCACAGTCTT TTGATTCACC CTATCAGCTC TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA
    GACTATTCAG GGGAGTACAC CGTCTTCCTC ATTCCCTGCA CGGTACAGCC AACACAGGCA
    TGGGTGGACT CTGGGGACAA GCCTCTATCC TGCACAGCAC ATGCACCAGA GAAATTCCTG
    CTGCCCATCG CCTTCCAGCA GACCAACCGC CCTGTCCCTG TTGTCTACTC TCTGAATACT
    GAGTTCCAGC TTTGTAATAA TGAGAAAGTT TTTCTAATGG ATCCTGCTCT CGCAGACATG
    TCTGTGGCTG AGATGGATTA CAAAGGAGCC TTTTCAATGG GTCAAACCCT GTATGGCAGA
    GTCCTGTGGA ATCCTGACCA GAACCTGAAT TCAGCCTACA AGCTGCAGCT GGAGAAAGTG
    TATTTGTGTA CGGGACGGGA TGGATATGTT CCCTTCTTTG ACCCTACAGG CACACTGTAT
    AATGAGGGCC CTCAGTATGG CTGCATTCAA CCAAACAAGC ACCTTAAACA CAGGTTCTTA
    CTTCTGGACC GAAAGCAGCC TGATGTATGT GATCGCTACT TCCATGATGT TCCCTTTGAG
    GCCAGCTTTG CATCTGACAT TCCTGACCTC CTGTCTTTGT CTGCGATGCC CGGTGTAGAC
    GGATTCACAA TGAAGGTTGA TGCCCTTTAT AAGGTTGAAG CAGGGCACCA ATGGTACCTC
    CAGGTCATCT ACGTTATTGG ACCCGAGTCT ATTTCGTCTC CCAGAATCCA GCGCTCAGTG
    ACTTATGAGC TGAAGCGAAG CCGCCGAGAT CTAGTAGACC GATCAGGTCG CCTAATGCTT
    GATGAATCAT TGATCTACGA CAATGAAGGT GACCAGGTAA AGAATGGCAC CAACATGAAG
    ACCTTGAAGC TTGAGATGGA ACCATCAGGC ACCTTCAACC CCCAAATGGG AGGATCCATT
    GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT TTCTTAGTCC TTCTAGCGTT CTGTTTCATG
    GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG AGGCCTTCAA AGACGGTGGA GGATTATCCT
    CTGAACACAA AGGTGGAGGT CTGCATTGAA CGTCTGGAGA AAAACCTCAA CAGCAAGCAC
    TGCTCTGTAA GAAACATCAA CGTTCTCAAC AAGAACCCCG ACACCTACAA AGTCAATGGG
    GTGAAAGTCA AACAGGTCAA TCTGGAGGTC AAACTCCACA ACAATCTAAA TGACGGCACT
    GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299931.1
    CDS365..12373
    Translation

    Target ORF information:

    RefSeq Version XM_009301656.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301656.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGT GGTTGGTGAA TCTCGCGTGA TCAGCACTGA TGTCCCCTTC
    ACTATTGAAG ACCTCAATTC AGACCATATC TTCTACGTGC AAAACATTCG CCAATCCAAT
    ATTCGACAGG ATGTCTTCAG CTTTTATATC AGTGATGGAT CAAGTCAAAC TGAGGCTTTT
    GATGTCACTA TAGATATTCA GCACACCAAA GAGGACCTAG TTCCAATTAT CTCTGTAAAT
    AGCATCCACG TGGAGGAGAA CTCTGGTGTG GTCATTACCA ACTCCTCCCT TAACGTACTG
    GACCGGGACA CGCCAGAGAA TGAGCTTTTG CTTACCATCA TCAAGAAGCC TTCATACGGG
    AAGATACGCA GAAGGCAGTT TTACTCAGAC CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG
    GGATCCACCT TCACCTACCA GGACATTCTT GATGAGCTGC TGGTCTACAC TCCAGACGGA
    CGTGGCTCTG GGACTGATGA GATCCAGTTT TCAGTAACAG ACGGCATATA CACGGAGACG
    GGCCGTCTGG AGTTCAGTAT GGATATTAGG AAGAAAGAAG GCCCTAGAGT AACAATCAAC
    AGAGGACTGC AGCTCGCAGC AGGTTCTTCA TCTAAAATCA CGGAGCAGCA TCTGAAAGGA
    ACGGACATAG ATTCAGATAA CCTAAAGCTG AGGTTCACCC TCATCAAAGA CCTAAGTGTG
    GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC AAGGCTCGCG TATCCGTGAA AGGACCCGTC
    ACCAGCTTCA CCCAGGATGA CATCAACAAA GGACATGTGG AATACATTCA TGAAAAAGGA
    GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC AACCTAGAAG ATCCTGAGGG AAACAGAGTC
    ATTGACCAGT CTTTCTTCAT TAGTGTTCTG GAGGACAGAC TTCCTCCCAC AGTGGTGGTT
    AATAAGGGTT TAGTTTTGGA TGAGAACTCC TTGAAGAAGA TCACAACTCT GCAGCTCTCC
    TCTACTGATC AGGATAGTGA ACCCGATGAA CTCGTTTACC GAATCACTAA ACAGCCCCAG
    CTTGGTCATC TAGAACATTC TAACAAACCA GGAACACGGA TCAGCTCGTT TACCCAGGCT
    GACCTGGCCT CTCGCAGCAT TCAGTATGTG CACACCAGTG AAGACGAGAT TCACTCCGAT
    GACTTCACCT TCACTGTATC TGATGGAGGA AACGAGGTCT CACAGACGTT CTACATCACC
    ATCCGGCCGG TGGACGATGC TCTGCCTGTG TTGCAGGTGC CCGGCATGAG GGTGCAGGAG
    GGCGTGAGGA AGACCATCAC TGAGTTTGAG CTCAAGGCCA CCGATGCTGA CACTGAGGAG
    GACTCTGTCA CTTTCACCAT TGTTCAGGCA CCTCGTCATG GGACCATCGA GCGCACCAGT
    AATGGTCAGC ATTATCGTCA GACAAACACC TTCACTATGG ACGACGTCTA CCAGAACCGC
    ATCAGTTACA ACCATGATGG CAGTAACTCC CTCAAAGATC GATTCACCTT CACTGTCGGT
    GACGGCACCA ACATGTTCTT CATCATCGAG GAGGGTGGAA AAGAGGTTGT CACTGCTGCT
    CCTCAAAAGT TTAAAATTGA CATTTTACCT GTTGATGACG GGACCCCTCG TATTGTTACC
    AACCTGGGCC TGCAGTGGCT GGAGTACATG GAAAATAAGG CCACTAATCT GATCTCCAAG
    AAGGAGCTTT TCACTATCGA TCCAGACACA GATGATGGAC AGCTGACTTA TGAAATCACT
    ACAGAAGCTA AGCACGGCTA CCTGGAGAGT AAACTCAATC CTGGCAAACC TATCACCTCT
    TTCACACAAG CTGACATAAA CCTTGGCCTT ATCCGATACG TTCTAAGCGA GGACAATGTT
    CAGGAGACCA TGGACAACTT CAAGTTCCTT GTAAAGGACA GCAAACCTAA CATCGTCAGT
    GACAATGTGT TCCACATCCA GTGGTCTCTG ATCAGCTTTG AACACAAGAG TTTTAACGTG
    AGTGAGAAGG ATGGAACGGT GGCGGTCACA GTGAAGAGGA CTGGTAATCT GAACCAGTAT
    GCTATTGTGC TGTGTCGAAC TGAGCAGGGC ACTGCCACCT CCACCTCCAG CATGGGCTCT
    CGGGCCGGAC AGCAGGATTA TGTTGAATAC GCCGGACAGG TTCAGTTTGA TGAGAGAGAA
    GACACCAAGG TGTGCACCAT TGTGATCAAC GATGACCAGG TGTTTGAGAA CATCGAGAGC
    TTTAATGTGG AGCTGAGTAT GCCGGTGTAT GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA
    GTCAATATCA ATGACACGGA GGATGAGCCC ACTTTGCAGT TTGATAAGAA AACCTACCAC
    GTCAATGAGA GCACTGGTTT CATATTTGCT CCTGTTGAGA GAAAAGGTGA CACCAGCAGC
    ACAGTTTCTG CCCTGTGCTA CACTGTGCCT AAATCAGCTC GGGGCAGCAG TCTTCACGCT
    CTCGAGTCCG GCTCAGACTA CAAGACCCGT GGCATGAGTG GCGACAACAG ACTCATCTTT
    GGTCCAGGAG TGAGCATGTC CACCTGTGAC GTCAAGCTGA TCGATGACAG CGAGTATGAA
    CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA GACGCCTCAG ATAATGCAAG GATGGGCGAC
    GTCACTGTGG CCAAAGTCAT CATCGACGGG CCCAACGACG CCTCTACTGT GTTTCTGGGA
    AACGCATCAT ACACCTTCAG TGAAGATGCA GGCACCATTG AAATCCCTGT TCTGCGGCAG
    GGCAGTGATC TTTCATCAGT CACCTCAGTG TGGTGCGCCA CACGTCCTGC AGAACAAGAT
    TCAGCCACGC CTGGAGTCGA CTACATCCCC AGCTCCAAAA AAGTAGAGTT CAAACCAGGC
    AAGACCGAGG AGACATGCAG TTTGACCATA ATGGATGACA TCCAGAACCC AACAATAGAA
    GGGAAAGAAT CCTTCATTGT GTTTCTTAGC TCTGCTAATG GAGCGGTCCT GACTGAACCC
    TATGAAGCCT CTGTCTACAT CACTGACACC ACTCAAGACA TCCCAAGCAT GCAGTTTGAC
    AAGCTGGCAT ATACAGTAAA GGAAAAAGAT GGCGTCCTGC ACATCCCTAT TTTTAGGACA
    GGAGATCTGT CCTATAAGTC TTCTGTCCGC TGTTTCACCC GCACAATGTC TGCAATGGTT
    ATGGATGACT TTGAGGAGAG GAGAAATGCA GATGATTCGA GAATCACCTT TCAGAAAGGG
    GAGAAGGTGA AGAATTGTAC AGTACTGATC CAGGATGACT CCGTGTTTGA GCCTGAAGAG
    GAGTTCCAGG TCCACCTTGG AAGCCCACAG GGTGACCATT GGATTGGAGC CATGATTGGA
    GTCAACGACA TTGTGACTGT GACCATCACA AATGATGAAG ATGCACCTAC GATCGAGTTT
    GAACAAGCCA CATATCAAGT TCGTGAGCCA CCTGGTCCCG ATGGCATTGA GGTTTTGAAT
    ATCAAGGTAA TTCGTAAAGG AGACCTCGAC CGCACCTCCA AAATTCGATG CAGCACGAGG
    GATGGATCGG CCCAGTCAGG TGTGGACTAT AACCCAAAGA GTCGAGTGCT GAAGTACAGC
    CCTGGTGTGG ACCACATCAT GTTTAAGGTG GAGATTCTGT CCAATGAGGA CAGAGAGTGG
    CATGAGTCTT TCTCTTTGGT CCTTGGTCCT GACGACCCAG TTGAAGCTGT TCTTGGAGAG
    ATCATCATTG CAACAGTCAC TATTCTGGAT CAGGAGGCTG CAGGAAGTCT TATCCTTCCG
    GCTCCACCCA TTGTGGTGTC TCTATCAGAT TATGACTACG TTCAGGAGGT CACTAAAGAG
    GGCAGCAAGA AGTCTCCGTC TCCTGGATAT CCGCTGGTCT GTGTGACCCC CTGTGACCCC
    CACTACCCCA AATACTCCGT CATGAAGGAG CGCTGTGAGG AGGCAGGCAT CAACCAGACT
    TCTCTACACT TCAGCTGGGA GGTGGCAACT CCTACAGACA CCAGCGGAGC AAGATCTCCC
    TTCGAGACTG TGACCGACAA CACACCCTAC ACCACAATCA ACCACATGGT CCTGGACAGC
    ATTTATTTCA GCAGGCGCTT CCATGTGCGC TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA
    CACCTGGGAA CTCCACTGAG GAGCAACATT GTCACCATTG GCACTGAGGG ATCAATATGC
    CACACTCCAG TCACCACAGG GACCGCTCGA GGCTTCCAAG CCCAGTCCTT TATCGCCACT
    CTAAAATACC TGGATGTCAA ACACAAAGAG CATCCAAACA GGATCCACAT TTCAGTTCAG
    ATTCCCCATC AGGATGGGAT GCTTCCACTG GTGTCCACCA TGCCTCTGCA TAACCTGCAC
    TTCCTGCTCT CTGAGTCCAT TTACCGTCAG CAACACGTCT GCTCCAACCT GGTCACACTT
    AAAGACCTGC AGGGTATCTC TGAGACCGGC TTCTTGGATG ACGTGTCATA TGACAGCATA
    TCCCTGGGTC CTGGATATGA CCGGCCATAT CAGTTCAACC CCAATGTTCG AGAACCCAGA
    ACCATACAGC TGTACAAGCA TCTCAATCTG AAGAGCTGCA TCTGGACATT CGATGCTTAT
    TATGACATGA CGGAGCTTAT CGATGTTTGC GGAGGATCAG TAACTGCCGA CTTTCAGGTC
    AGAGACTCGG CTCAGTCCTT CCTGACAGTC CAGGTTCCGC TCTATGTGTC CTACATCTAC
    GTGACGGCTC CTCGTGGATG GGCGTCTCTG GAGCACCACA CAGAAATGGA GTTCTCCTTC
    TTCTACGACA CTGTCTTATG GAGAACAGGC ATTCAGACAG ACAGCGTGCT TTCGGCCAGA
    TTACAGATCA TTCGGATCTT CATTAAGGAT GACGGACGAC TGGTGATTGA GTTTAAAACC
    CACGCCAAGT TCAGAGGTCA GTTTGTCCCA GAGCATCACA CCCTCCCCGG ACAGAAGTCT
    CATCTGATGG CTCCAGATCA CTTAGGAGGC ATGGAGTTCG ACCTACAGCT GGTGTGGAGC
    GCACAGTCTT TTGATTCACC CTATCAGCTC TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA
    GACTATTCAG GGGAGTACAC CGTCTTCCTC ATTCCCTGCA CGGTACAGCC AACACAGGCA
    TGGGTGGACT CTGGGGACAA GCCTCTATCC TGCACAGCAC ATGCACCAGA GAAATTCCTG
    CTGCCCATCG CCTTCCAGCA GACCAACCGC CCTGTCCCTG TTGTCTACTC TCTGAATACT
    GAGTTCCAGC TTTGTAATAA TGAGAAAGTT TTTCTAATGG ATCCTGCTCT CGCAGACATG
    TCTGTGGCTG AGATGGATTA CAAAGGAGCC TTTTCAATGG GTCAAACCCT GTATGGCAGA
    GTCCTGTGGA ATCCTGACCA GAACCTGAAT TCAGCCTACA AGCTGCAGCT GGAGAAAGTG
    TATTTGTGTA CGGGACGGGA TGGATATGTT CCCTTCTTTG ACCCTACAGG CACACTGTAT
    AATGAGGGCC CTCAGTATGG CTGCATTCAA CCAAACAAGC ACCTTAAACA CAGGTTCTTA
    CTTCTGGACC GAAAGCAGCC TGATGTATGT GATCGCTACT TCCATGATGT TCCCTTTGAG
    GCCAGCTTTG CATCTGACAT TCCTGACCTC CTGTCTTTGT CTGCGATGCC CGGTGTAGAC
    GGATTCACAA TGAAGGTTGA TGCCCTTTAT AAGGTTGAAG CAGGGCACCA ATGGTACCTC
    CAGGTCATCT ACGTTATTGG ACCCGAGTCT ATTTCGTCTC CCAGAATCCA GCGCTCAGTG
    ACTTATGAGC TGAAGCGAAG CCGCCGAGAT CTAGTAGACC GATCAGGTCG CCTAATGCTT
    GATGAATCAT TGATCTACGA CAATGAAGGT GACCAGGTAA AGAATGGCAC CAACATGAAG
    ACCTTGAAGC TTGAGATGGA ACCATCAGGC ACCTTCAACC CCCAAATGGG AGGATCCATT
    GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT TTCTTAGTCC TTCTAGCGTT CTGTTTCATG
    GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG AGGCCTTCAA AGACGGTGGA GGATTATCCT
    CTGAACACAA AGGTGGAGGT CTGCATTGAA CGTCTGGAGA AAAACCTCAA CAGCAAGCAC
    TGCTCTGTAA GAAACATCAA CGTTCTCAAC AAGAACCCCG ACACCTACAA AGTCAATGGG
    GTGAAAGTCA AACAGGTCAA TCTGGAGGTC AAACTCCACA ACAATCTAAA TGACGGCACT
    GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44084
    Clone ID Related Accession (Same CDS sequence) XM_009301656.2 , XM_009301656.3
    Accession Version XM_009301656.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product extracellular matrix protein FRAS1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301656.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
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    1801
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    1981
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    9781
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    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGT GGTTGGTGAA TCTCGCGTGA TCAGCACTGA TGTCCCCTTC
    ACTATTGAAG ACCTCAATTC AGACCATATC TTCTACGTGC AAAACATTCG CCAATCCAAT
    ATTCGACAGG ATGTCTTCAG CTTTTATATC AGTGATGGAT CAAGTCAAAC TGAGGCTTTT
    GATGTCACTA TAGATATTCA GCACACCAAA GAGGACCTAG TTCCAATTAT CTCTGTAAAT
    AGCATCCACG TGGAGGAGAA CTCTGGTGTG GTCATTACCA ACTCCTCCCT TAACGTACTG
    GACCGGGACA CGCCAGAGAA TGAGCTTTTG CTTACCATCA TCAAGAAGCC TTCATACGGG
    AAGATACGCA GAAGGCAGTT TTACTCAGAC CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG
    GGATCCACCT TCACCTACCA GGACATTCTT GATGAGCTGC TGGTCTACAC TCCAGACGGA
    CGTGGCTCTG GGACTGATGA GATCCAGTTT TCAGTAACAG ACGGCATATA CACGGAGACG
    GGCCGTCTGG AGTTCAGTAT GGATATTAGG AAGAAAGAAG GCCCTAGAGT AACAATCAAC
    AGAGGACTGC AGCTCGCAGC AGGTTCTTCA TCTAAAATCA CGGAGCAGCA TCTGAAAGGA
    ACGGACATAG ATTCAGATAA CCTAAAGCTG AGGTTCACCC TCATCAAAGA CCTAAGTGTG
    GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC AAGGCTCGCG TATCCGTGAA AGGACCCGTC
    ACCAGCTTCA CCCAGGATGA CATCAACAAA GGACATGTGG AATACATTCA TGAAAAAGGA
    GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC AACCTAGAAG ATCCTGAGGG AAACAGAGTC
    ATTGACCAGT CTTTCTTCAT TAGTGTTCTG GAGGACAGAC TTCCTCCCAC AGTGGTGGTT
    AATAAGGGTT TAGTTTTGGA TGAGAACTCC TTGAAGAAGA TCACAACTCT GCAGCTCTCC
    TCTACTGATC AGGATAGTGA ACCCGATGAA CTCGTTTACC GAATCACTAA ACAGCCCCAG
    CTTGGTCATC TAGAACATTC TAACAAACCA GGAACACGGA TCAGCTCGTT TACCCAGGCT
    GACCTGGCCT CTCGCAGCAT TCAGTATGTG CACACCAGTG AAGACGAGAT TCACTCCGAT
    GACTTCACCT TCACTGTATC TGATGGAGGA AACGAGGTCT CACAGACGTT CTACATCACC
    ATCCGGCCGG TGGACGATGC TCTGCCTGTG TTGCAGGTGC CCGGCATGAG GGTGCAGGAG
    GGCGTGAGGA AGACCATCAC TGAGTTTGAG CTCAAGGCCA CCGATGCTGA CACTGAGGAG
    GACTCTGTCA CTTTCACCAT TGTTCAGGCA CCTCGTCATG GGACCATCGA GCGCACCAGT
    AATGGTCAGC ATTATCGTCA GACAAACACC TTCACTATGG ACGACGTCTA CCAGAACCGC
    ATCAGTTACA ACCATGATGG CAGTAACTCC CTCAAAGATC GATTCACCTT CACTGTCGGT
    GACGGCACCA ACATGTTCTT CATCATCGAG GAGGGTGGAA AAGAGGTTGT CACTGCTGCT
    CCTCAAAAGT TTAAAATTGA CATTTTACCT GTTGATGACG GGACCCCTCG TATTGTTACC
    AACCTGGGCC TGCAGTGGCT GGAGTACATG GAAAATAAGG CCACTAATCT GATCTCCAAG
    AAGGAGCTTT TCACTATCGA TCCAGACACA GATGATGGAC AGCTGACTTA TGAAATCACT
    ACAGAAGCTA AGCACGGCTA CCTGGAGAGT AAACTCAATC CTGGCAAACC TATCACCTCT
    TTCACACAAG CTGACATAAA CCTTGGCCTT ATCCGATACG TTCTAAGCGA GGACAATGTT
    CAGGAGACCA TGGACAACTT CAAGTTCCTT GTAAAGGACA GCAAACCTAA CATCGTCAGT
    GACAATGTGT TCCACATCCA GTGGTCTCTG ATCAGCTTTG AACACAAGAG TTTTAACGTG
    AGTGAGAAGG ATGGAACGGT GGCGGTCACA GTGAAGAGGA CTGGTAATCT GAACCAGTAT
    GCTATTGTGC TGTGTCGAAC TGAGCAGGGC ACTGCCACCT CCACCTCCAG CATGGGCTCT
    CGGGCCGGAC AGCAGGATTA TGTTGAATAC GCCGGACAGG TTCAGTTTGA TGAGAGAGAA
    GACACCAAGG TGTGCACCAT TGTGATCAAC GATGACCAGG TGTTTGAGAA CATCGAGAGC
    TTTAATGTGG AGCTGAGTAT GCCGGTGTAT GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA
    GTCAATATCA ATGACACGGA GGATGAGCCC ACTTTGCAGT TTGATAAGAA AACCTACCAC
    GTCAATGAGA GCACTGGTTT CATATTTGCT CCTGTTGAGA GAAAAGGTGA CACCAGCAGC
    ACAGTTTCTG CCCTGTGCTA CACTGTGCCT AAATCAGCTC GGGGCAGCAG TCTTCACGCT
    CTCGAGTCCG GCTCAGACTA CAAGACCCGT GGCATGAGTG GCGACAACAG ACTCATCTTT
    GGTCCAGGAG TGAGCATGTC CACCTGTGAC GTCAAGCTGA TCGATGACAG CGAGTATGAA
    CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA GACGCCTCAG ATAATGCAAG GATGGGCGAC
    GTCACTGTGG CCAAAGTCAT CATCGACGGG CCCAACGACG CCTCTACTGT GTTTCTGGGA
    AACGCATCAT ACACCTTCAG TGAAGATGCA GGCACCATTG AAATCCCTGT TCTGCGGCAG
    GGCAGTGATC TTTCATCAGT CACCTCAGTG TGGTGCGCCA CACGTCCTGC AGAACAAGAT
    TCAGCCACGC CTGGAGTCGA CTACATCCCC AGCTCCAAAA AAGTAGAGTT CAAACCAGGC
    AAGACCGAGG AGACATGCAG TTTGACCATA ATGGATGACA TCCAGAACCC AACAATAGAA
    GGGAAAGAAT CCTTCATTGT GTTTCTTAGC TCTGCTAATG GAGCGGTCCT GACTGAACCC
    TATGAAGCCT CTGTCTACAT CACTGACACC ACTCAAGACA TCCCAAGCAT GCAGTTTGAC
    AAGCTGGCAT ATACAGTAAA GGAAAAAGAT GGCGTCCTGC ACATCCCTAT TTTTAGGACA
    GGAGATCTGT CCTATAAGTC TTCTGTCCGC TGTTTCACCC GCACAATGTC TGCAATGGTT
    ATGGATGACT TTGAGGAGAG GAGAAATGCA GATGATTCGA GAATCACCTT TCAGAAAGGG
    GAGAAGGTGA AGAATTGTAC AGTACTGATC CAGGATGACT CCGTGTTTGA GCCTGAAGAG
    GAGTTCCAGG TCCACCTTGG AAGCCCACAG GGTGACCATT GGATTGGAGC CATGATTGGA
    GTCAACGACA TTGTGACTGT GACCATCACA AATGATGAAG ATGCACCTAC GATCGAGTTT
    GAACAAGCCA CATATCAAGT TCGTGAGCCA CCTGGTCCCG ATGGCATTGA GGTTTTGAAT
    ATCAAGGTAA TTCGTAAAGG AGACCTCGAC CGCACCTCCA AAATTCGATG CAGCACGAGG
    GATGGATCGG CCCAGTCAGG TGTGGACTAT AACCCAAAGA GTCGAGTGCT GAAGTACAGC
    CCTGGTGTGG ACCACATCAT GTTTAAGGTG GAGATTCTGT CCAATGAGGA CAGAGAGTGG
    CATGAGTCTT TCTCTTTGGT CCTTGGTCCT GACGACCCAG TTGAAGCTGT TCTTGGAGAG
    ATCATCATTG CAACAGTCAC TATTCTGGAT CAGGAGGCTG CAGGAAGTCT TATCCTTCCG
    GCTCCACCCA TTGTGGTGTC TCTATCAGAT TATGACTACG TTCAGGAGGT CACTAAAGAG
    GGCAGCAAGA AGTCTCCGTC TCCTGGATAT CCGCTGGTCT GTGTGACCCC CTGTGACCCC
    CACTACCCCA AATACTCCGT CATGAAGGAG CGCTGTGAGG AGGCAGGCAT CAACCAGACT
    TCTCTACACT TCAGCTGGGA GGTGGCAACT CCTACAGACA CCAGCGGAGC AAGATCTCCC
    TTCGAGACTG TGACCGACAA CACACCCTAC ACCACAATCA ACCACATGGT CCTGGACAGC
    ATTTATTTCA GCAGGCGCTT CCATGTGCGC TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA
    CACCTGGGAA CTCCACTGAG GAGCAACATT GTCACCATTG GCACTGAGGG ATCAATATGC
    CACACTCCAG TCACCACAGG GACCGCTCGA GGCTTCCAAG CCCAGTCCTT TATCGCCACT
    CTAAAATACC TGGATGTCAA ACACAAAGAG CATCCAAACA GGATCCACAT TTCAGTTCAG
    ATTCCCCATC AGGATGGGAT GCTTCCACTG GTGTCCACCA TGCCTCTGCA TAACCTGCAC
    TTCCTGCTCT CTGAGTCCAT TTACCGTCAG CAACACGTCT GCTCCAACCT GGTCACACTT
    AAAGACCTGC AGGGTATCTC TGAGACCGGC TTCTTGGATG ACGTGTCATA TGACAGCATA
    TCCCTGGGTC CTGGATATGA CCGGCCATAT CAGTTCAACC CCAATGTTCG AGAACCCAGA
    ACCATACAGC TGTACAAGCA TCTCAATCTG AAGAGCTGCA TCTGGACATT CGATGCTTAT
    TATGACATGA CGGAGCTTAT CGATGTTTGC GGAGGATCAG TAACTGCCGA CTTTCAGGTC
    AGAGACTCGG CTCAGTCCTT CCTGACAGTC CAGGTTCCGC TCTATGTGTC CTACATCTAC
    GTGACGGCTC CTCGTGGATG GGCGTCTCTG GAGCACCACA CAGAAATGGA GTTCTCCTTC
    TTCTACGACA CTGTCTTATG GAGAACAGGC ATTCAGACAG ACAGCGTGCT TTCGGCCAGA
    TTACAGATCA TTCGGATCTT CATTAAGGAT GACGGACGAC TGGTGATTGA GTTTAAAACC
    CACGCCAAGT TCAGAGGTCA GTTTGTCCCA GAGCATCACA CCCTCCCCGG ACAGAAGTCT
    CATCTGATGG CTCCAGATCA CTTAGGAGGC ATGGAGTTCG ACCTACAGCT GGTGTGGAGC
    GCACAGTCTT TTGATTCACC CTATCAGCTC TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA
    GACTATTCAG GGGAGTACAC CGTCTTCCTC ATTCCCTGCA CGGTACAGCC AACACAGGCA
    TGGGTGGACT CTGGGGACAA GCCTCTATCC TGCACAGCAC ATGCACCAGA GAAATTCCTG
    CTGCCCATCG CCTTCCAGCA GACCAACCGC CCTGTCCCTG TTGTCTACTC TCTGAATACT
    GAGTTCCAGC TTTGTAATAA TGAGAAAGTT TTTCTAATGG ATCCTGCTCT CGCAGACATG
    TCTGTGGCTG AGATGGATTA CAAAGGAGCC TTTTCAATGG GTCAAACCCT GTATGGCAGA
    GTCCTGTGGA ATCCTGACCA GAACCTGAAT TCAGCCTACA AGCTGCAGCT GGAGAAAGTG
    TATTTGTGTA CGGGACGGGA TGGATATGTT CCCTTCTTTG ACCCTACAGG CACACTGTAT
    AATGAGGGCC CTCAGTATGG CTGCATTCAA CCAAACAAGC ACCTTAAACA CAGGTTCTTA
    CTTCTGGACC GAAAGCAGCC TGATGTATGT GATCGCTACT TCCATGATGT TCCCTTTGAG
    GCCAGCTTTG CATCTGACAT TCCTGACCTC CTGTCTTTGT CTGCGATGCC CGGTGTAGAC
    GGATTCACAA TGAAGGTTGA TGCCCTTTAT AAGGTTGAAG CAGGGCACCA ATGGTACCTC
    CAGGTCATCT ACGTTATTGG ACCCGAGTCT ATTTCGTCTC CCAGAATCCA GCGCTCAGTG
    ACTTATGAGC TGAAGCGAAG CCGCCGAGAT CTAGTAGACC GATCAGGTCG CCTAATGCTT
    GATGAATCAT TGATCTACGA CAATGAAGGT GACCAGGTAA AGAATGGCAC CAACATGAAG
    ACCTTGAAGC TTGAGATGGA ACCATCAGGC ACCTTCAACC CCCAAATGGG AGGATCCATT
    GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT TTCTTAGTCC TTCTAGCGTT CTGTTTCATG
    GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG AGGCCTTCAA AGACGGTGGA GGATTATCCT
    CTGAACACAA AGGTGGAGGT CTGCATTGAA CGTCTGGAGA AAAACCTCAA CAGCAAGCAC
    TGCTCTGTAA GAAACATCAA CGTTCTCAAC AAGAACCCCG ACACCTACAA AGTCAATGGG
    GTGAAAGTCA AACAGGTCAA TCTGGAGGTC AAACTCCACA ACAATCTAAA TGACGGCACT
    GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299931.1
    CDS358..12366
    Translation

    Target ORF information:

    RefSeq Version XM_009301656.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Fraser extracellular matrix complex subunit 1 (fras1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301656.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    ATGGGTCCTA TTAGGATCCT TCTTCTAACC ATTTTAATTG GATATAGCAC AATCTCGCCC 
    GGAGCTGAAG GAGCGTGTGT CCATCAAGGC TCATTGTTTG CGAATAATAC ATCATGGAAA
    CCAGACACCT GTCAAGACTG CATCTGCTAC AATGATATTG TCATCTGCAA ACCAACGCGC
    TGCCGAAATC CCCAGTGTGA CTTTCCGAGG GGTGAGCGTT TAAGAATCCC ACCTAACAAG
    TGTTGTCCTG AATGCTTTGC GCCGTCGCCC GGCATCTGTC ACCATGAAGG ACTGGTTTAT
    GGGCATGATA GTCAATGGAG TAACTCCAAA TGTTCAGTGT GCACATGTGC TCATGGGAAG
    GTGACCTGTG GGCCACGCGC CTGTCCTCCT CTCAGCTGTG GGAAAGACCA GAGTCCTTTT
    ATCCCAGATG GAAACTGCTG TCCAGTTTGT GCTCATAATG GAGTCTCTTG CTCCTGGGAA
    GGCCGTGAGT ACAGAGATGG GTTGGAGTGG ACGTCAAGCC CCTGTACAAA GTGCAGGTGC
    AGAAACGGAC ACACTGAATG CCTGGTGGCT GAGTGTCAGC CGGTTACATG CAAAGTGAAT
    GAGAACCTGG TGGTCCATCC AGGACAGTGC TGTCCACAGT GTGAGCCAAA TCCCTGTATG
    GAGGCTGGGA ATGAGTACAA GCATGGCGAG CAGTGGCAGA GGAGCAGCTG TACAACCTGT
    GTGTGTGATC GTGGTCATTC TCGCTGTCAA ACTGAGAAGT GTCCTCCTCT CCATTGCGAT
    AAGGGTCAGA CGAAGGTCAA GCGAGCCGAC CAGTGCTGTG AAGACTGTGC CACATCTAAA
    GGCAGCTGCC TCTATGAGGG CATTGTTCGT TACCATGGCG ACATGTGGAA CGGCACAGGC
    TGTGAGTTCT GCATGTGTGA ACGGGGTCAG GTGCTGTGCC AACGGGTCGA GTGCTCCAGA
    TCAGAGTGCC CACGGGGAGA AAAACTGGTC CATTTGCCTG GAAAGTGCTG CCCAGAGTGT
    AAGACGACAA CCAGTTCCTG TACATATTCA CAGCCAGACC AAAAGGGGCA GAAAGCCATC
    AAGAGTTTTA GACGTCTCTC TAATCTAGAG ATTGTCAGAG AAGGGTTGTG TCGAGAGTGC
    CAGTGTCAAG AGGGTCACGT GACCTGCTAC CAGCACTCCT GTCCCACCTG TCCACTGGGA
    ACACTAACTA TACCTCACCG GGAGCAGTGC TGTCCCGACT GCAATCCAGA AGTAAAGTGC
    CACGAGGACT GTCTGACATG CTCTGGCACA CCGGATCACT GTGCCAGCTG TCGAGACCCT
    TCTGCCCTGC TAATGAACGG ACGCTGTCTG CAGGCCTGTC CTCAAGGCTT TTTCGCTCAG
    GGCAAGGTTT GTGCAGCCTG CCAGCCGTCA TGTGCCACCT GTGAGAATGA CTTTGAATGC
    ACAGCGTGCG GTGGGTCGTT TCTGCTCAGT GGCAGGCAGT GTGTGGCCAC CTGTGAGAGA
    GGTCTGTTTC AGGCCCACAC ACACTGCCTC AGTTGCCATG ACTTATGCTC ATCGTGTCAG
    GGGGCGGGGC CTCAGGATTG TTTGACATGC TCAGACTCCT CCCACTTACT GAAGGATGGA
    TACTGTGTTT CAGACTGCGG CCCAGGCTTT TACACAAGTC AAGGCAACTG TTACGCTTGT
    GACCCTTCCT GTGCAACCTG CCAACCTGAC AGCCCCAGCT GTATGAGCTG CCCTGCAGAA
    CATGCACTGC ATCAAGGGAA ATGTGCACCA CAATGTCCAG CGCAGTATTA CAAGGATGAC
    CACGGCCGAT GCCAAGCTTG CCACAGTTCG TGTGCGTCCT GCTCGGGGCC TGCTGTGTCT
    CACTGCACCT CCTGCTCAAA GGCTCTGATC TTGAACCAGG GCCAGTGTGT GGAGTCCTGT
    GGAGAGGGCC TGTTCTCCAG AGCGGGACAC TGTCACAGTT GTCATTCGTC CTGTAGGGTC
    TGTGTGGGTC CAGACTCCTC AGACTGCACA CAGTGTGTCA AAATGGAAGA GGTGCTGCAG
    CCTCAAAATG GACATGTCTC TCACGGGTCC TGCATGTCCG CCTGCAGGCC ACAGCATTAC
    CTTGAAACAG ATCGGACCTG CAGAGAATGC CACAGCTCAT GTTCAGGCTG CATTGGTGGA
    AGTTTTCAGA ACTGTACCTC CTGTTTGCGA CCCGGTGTCC TTCATCAAGG GCAGTGCCTC
    AGCAAATGTC CCAATGGCTT CTTCCACATT CAGGACCTCA CTTGTCAAGC TTGTCATCCA
    TCATGTAAGG AGTGCACTGG CACATCTCAG GCCGACTGTT CTGCCTGTCC CGCTCATGCC
    AGTCTTCATA ACGGTTACTG CCGAACCAGC TGCCCCGAGG GCCAGTACCT CAATTCTGTG
    GGTTTCTGCG ATGAGTGCGA GGAGGGATGC CAGCGCTGTG TCGCAGACCT GCAGTCTGGC
    TCTGGCACTG TGTGTTTGTG GTGTAAAGTT CCCAGAATGC TTCTGCTGGG CAATCACTGT
    GTTCCCGAGT GCCCACACAA ACACTACCGC TGGCATGGAG CCTGCAAAAA ATGTCACTCT
    TCCTGTGAGG AGTGCAGTGC TGATGGTCCT CTTTCTTGTA CCTCTTGTTT GGCCCCTGAA
    GTTTTGGCCC CATCAGGACT CTGTAGTCCA CGCTGTCCGA CTGGATATTA TGCTGATGAA
    AACCGAGTTT GCCAAAAGTG TGATTCTCAG TGTCTGAGCT GTGAGATGGC AGGCGTGTGC
    TCGTCCTGTA GAGATCCAGC TAAAGTGCTT CTGTTTGGGG AGTGTCAGTA TGAGAGCTGT
    GCTCATCAGT ATTACCTCAA CACCAGCACA CGCACATGCA GAGAATGCGA CTGGAGCTGT
    AATGCGTGCC GTGGACCTCT GCGCAGTGAC TGCCTGCAGT GCATGGAGGG ATACGTCCTG
    CAGGATGGCA TTTGTGTGCC GGGTTGCTCA CCGGGATTCT ACCAGGATGC AGAGAGATGT
    CTGAGCTGTG AAGAGCACTG TGAGCAGTGC CAGGCTCATG GTCAGTGCCA GCGGTGCCAG
    TTGCCGTATG CCTCATTTAA CGGACATTGT GTGCTGGAAT GCAGAAAGCA CTATTTTCTA
    GATGACTCGA CTCGACAGTG CACCGCATGC TCAGCTGGTT GTGTGGAGTG TGTGAGTGCA
    GGCGAGTGTC GCGTCTGTGC TGAAAACACC TTCCTGAAGG ATGGCAGATG CATCCCAGAC
    TGTGGTCATG GATATTATGC TGACCTAAAA ACAAGAGCTT GTCATGCAAA TAGTCGTGCT
    CCTTCTCTTT ACATCAATGG CTCTCTGCTG GTTCCTATTG GTGGAGTTAA GCCTCTGGAT
    CCAGCACTCC TGTCAATTAA GGATGCAGAT AGCCAATCAG AGAGCATGTT GCTTCAGCTC
    CTTCAGCCGC CCAGTAATGG CCACCTCAGG CTGCTGGAGA ATGACAGAGA GAGATCGCTG
    GGCAAAGATG ACACTTTCAC CTATACTCAG CTCAAACGCA GAGCGGTGCG CTTCATTCAC
    GACAAAGAGC AGACCAAAAG TGGGCAGTTC ACGTTAAGGG CAGCAGATCC TCAACTGTTC
    TCTCAGCCTC AGACTGTTAC TGTGCAGGCT CTCTCTCAGC AGCCACCCAC TGTAATCACC
    AACCAAATCC TGTACATTAA CTCTGGCGAG ACAGCCGTCA TCAGCAGATC TATTCTACAC
    ATCAGCGACC TTGACAATCC TCAGGACGTG TTCATCACGG TTCTGGATCC ACCTCAGCAC
    GGTCGCCTGA CATACATGCA CAGTGATACG CAGGTCACCC ATTTTAAACT GGAAGAGCTA
    GATGGCGAGC AGCTGCAGTA TGTTCATGAT CGTTCAGATG GAGACCAGGA CAGACTTCTG
    CTGCAGGTCA ATGACGGACA CAGCTACCAG AACGTCCTGT TCCACATCAG CATTGAACAG
    AAGGTTGGAT TGACTCCGCA CCTGCGTTCA GTGCCCAAAA CTTGGGTGAA GGAGGGAGGA
    ATGGTGCAGC TTTCTAAAAA GTGTCTCAAT GCTCAATTCA AAGGAGCCAG TGACTCTGAG
    ATCCTGTACA GCATCCATTC TGAGAGCGGA CAGCCCAAAT ACGGAGAGAT TGTGCTGGTG
    TCTATGCCCG CTGACGGTCC TGTGGAAGCG TGGCAAGCCT TTTCTGATGG TCAAGACTCT
    ACACCCACCA GCTCATTTAC ACAGCAAGAT GTAAATGAAG GCACTGTCTG GTACCGACAT
    TATGGATCTG GAACACAGAG AGACACTTTT CAGTTTCAGG TTTCAAGTGA GATCTATCCA
    GAGACTCTGA GCGATACACA GACCTTCACT ATAGGAGTGA TGCCTCAGAG CCCTGGCATG
    CCTCAGCTTT CACCTGGAGT CGATCTGCAG ATCACTGCGC TGGAAGATCG CGTGACTGTG
    ATCTCTCCAT CTGCTCTCTC CTTCACTGAC TCTGAGACAC CCAGCGACAA GCTTGTCTAC
    AACATCACCA AAGCGCTCAC CTCAGGCCAC GGTGTGCTGG AGCACAAGGA CAGACCTTAT
    CATCATGTGC GTCTCTTCAC CCAGGCTGAC GTAGATTCTG GCAAAATTAT CTACAGACCC
    CCACAGGCCC CATCTCATCT TCAGGAGCTT TATCAATACT CATTTACAGG TCTGCCAGAA
    TCAGTGAGCG TCCATTTCAC AGTGTCTGGT GGAGAACACA CCACCCCAGA GATGGACTTT
    GCCATCCTGC TTTTGGTCAA TCATCAGCAG CCACCAGTCT TTCAGATCCT GGACCCAGTG
    CTGGAGGTCA GCCTTGGCGG CAGGGCACCA GTGGGTGTGC AACAGTTGGC AGTAACAGAT
    TCAGACACGG CCCCTGAAGA GCTGGAGTTT GAGGTGGTGG AGGGACCACT ACATGGGACG
    CTCCTGAAAG TGGACCTTGG ATCCCAGGTC CAGATGGTCA ATGGGGACAC CTTTACCTCC
    AGTGATGTCA CCAGAAACAT CCTGCAGTAT GAACATGCAG GGCTCACCAC TGAAGAGGAC
    TCCATGACCT TCTCAGTGAC TGACGGGATC TCTATGACCT CCATCGCGGT GCAGGTTTTG
    GTGTCAGAGG TCAAAGGCGA CGCACCCAAG CGTGACCCAA AGGCTTTTCT TTCCATGGAA
    GTTGCAGAGA AGAGCAGCAG CATCATTAGA CGTTCACATT TAGCTTATAT GGATGATGCA
    TCTCCAGATG AGAGGATTTT TGTGCAGTTA GTCTCTGTGC CAATGTATGG GATCCTTACA
    AAAAATAAAG CTGGAGCAGA CTATGAGGAG CTTAGTGAAT ATTCTTCCTT TACAATGGAG
    GACATCAACA ACCACAAAAT AAGGTATGTT ACGTCCTTTG AAACTGGGAA CCAGCCTGTG
    ACTGACATTT TCCACTTCGT CGTCTATGAT GGTGATAACA ATCGTCTGGA AAACCAGATG
    TTCACTATCA CTATCACCAC TACCCAGAGG CAGCCACCTG TTGTCAGCGT CCGCAGTGGC
    ATTAAAGTTC AGGAAGGTGG GAGAGTCCAG CTCTCAACCA ATCACATTAT TGCATCTGAC
    CCAGACACAC CCAGAAAGGA CCTGCTGGTT TGGCTGATCA GACCACCTAA ATTTGGCTTC
    ATTGAGAACA CAAAGCAAGT GGTTGGTGAA TCTCGCGTGA TCAGCACTGA TGTCCCCTTC
    ACTATTGAAG ACCTCAATTC AGACCATATC TTCTACGTGC AAAACATTCG CCAATCCAAT
    ATTCGACAGG ATGTCTTCAG CTTTTATATC AGTGATGGAT CAAGTCAAAC TGAGGCTTTT
    GATGTCACTA TAGATATTCA GCACACCAAA GAGGACCTAG TTCCAATTAT CTCTGTAAAT
    AGCATCCACG TGGAGGAGAA CTCTGGTGTG GTCATTACCA ACTCCTCCCT TAACGTACTG
    GACCGGGACA CGCCAGAGAA TGAGCTTTTG CTTACCATCA TCAAGAAGCC TTCATACGGG
    AAGATACGCA GAAGGCAGTT TTACTCAGAC CCTTTAGAGA ATGGCAGGAT TCTTCAGCAG
    GGATCCACCT TCACCTACCA GGACATTCTT GATGAGCTGC TGGTCTACAC TCCAGACGGA
    CGTGGCTCTG GGACTGATGA GATCCAGTTT TCAGTAACAG ACGGCATATA CACGGAGACG
    GGCCGTCTGG AGTTCAGTAT GGATATTAGG AAGAAAGAAG GCCCTAGAGT AACAATCAAC
    AGAGGACTGC AGCTCGCAGC AGGTTCTTCA TCTAAAATCA CGGAGCAGCA TCTGAAAGGA
    ACGGACATAG ATTCAGATAA CCTAAAGCTG AGGTTCACCC TCATCAAAGA CCTAAGTGTG
    GGCTCCCTGA TGCTGAAAAC AGGCAGAAAC AAGGCTCGCG TATCCGTGAA AGGACCCGTC
    ACCAGCTTCA CCCAGGATGA CATCAACAAA GGACATGTGG AATACATTCA TGAAAAAGGA
    GAGAGCGGAG GAAGCTTCTC TTTTAAGTTC AACCTAGAAG ATCCTGAGGG AAACAGAGTC
    ATTGACCAGT CTTTCTTCAT TAGTGTTCTG GAGGACAGAC TTCCTCCCAC AGTGGTGGTT
    AATAAGGGTT TAGTTTTGGA TGAGAACTCC TTGAAGAAGA TCACAACTCT GCAGCTCTCC
    TCTACTGATC AGGATAGTGA ACCCGATGAA CTCGTTTACC GAATCACTAA ACAGCCCCAG
    CTTGGTCATC TAGAACATTC TAACAAACCA GGAACACGGA TCAGCTCGTT TACCCAGGCT
    GACCTGGCCT CTCGCAGCAT TCAGTATGTG CACACCAGTG AAGACGAGAT TCACTCCGAT
    GACTTCACCT TCACTGTATC TGATGGAGGA AACGAGGTCT CACAGACGTT CTACATCACC
    ATCCGGCCGG TGGACGATGC TCTGCCTGTG TTGCAGGTGC CCGGCATGAG GGTGCAGGAG
    GGCGTGAGGA AGACCATCAC TGAGTTTGAG CTCAAGGCCA CCGATGCTGA CACTGAGGAG
    GACTCTGTCA CTTTCACCAT TGTTCAGGCA CCTCGTCATG GGACCATCGA GCGCACCAGT
    AATGGTCAGC ATTATCGTCA GACAAACACC TTCACTATGG ACGACGTCTA CCAGAACCGC
    ATCAGTTACA ACCATGATGG CAGTAACTCC CTCAAAGATC GATTCACCTT CACTGTCGGT
    GACGGCACCA ACATGTTCTT CATCATCGAG GAGGGTGGAA AAGAGGTTGT CACTGCTGCT
    CCTCAAAAGT TTAAAATTGA CATTTTACCT GTTGATGACG GGACCCCTCG TATTGTTACC
    AACCTGGGCC TGCAGTGGCT GGAGTACATG GAAAATAAGG CCACTAATCT GATCTCCAAG
    AAGGAGCTTT TCACTATCGA TCCAGACACA GATGATGGAC AGCTGACTTA TGAAATCACT
    ACAGAAGCTA AGCACGGCTA CCTGGAGAGT AAACTCAATC CTGGCAAACC TATCACCTCT
    TTCACACAAG CTGACATAAA CCTTGGCCTT ATCCGATACG TTCTAAGCGA GGACAATGTT
    CAGGAGACCA TGGACAACTT CAAGTTCCTT GTAAAGGACA GCAAACCTAA CATCGTCAGT
    GACAATGTGT TCCACATCCA GTGGTCTCTG ATCAGCTTTG AACACAAGAG TTTTAACGTG
    AGTGAGAAGG ATGGAACGGT GGCGGTCACA GTGAAGAGGA CTGGTAATCT GAACCAGTAT
    GCTATTGTGC TGTGTCGAAC TGAGCAGGGC ACTGCCACCT CCACCTCCAG CATGGGCTCT
    CGGGCCGGAC AGCAGGATTA TGTTGAATAC GCCGGACAGG TTCAGTTTGA TGAGAGAGAA
    GACACCAAGG TGTGCACCAT TGTGATCAAC GATGACCAGG TGTTTGAGAA CATCGAGAGC
    TTTAATGTGG AGCTGAGTAT GCCGGTGTAT GCCCTCCTGG GCTCAGTGAC CCGAGCTAAA
    GTCAATATCA ATGACACGGA GGATGAGCCC ACTTTGCAGT TTGATAAGAA AACCTACCAC
    GTCAATGAGA GCACTGGTTT CATATTTGCT CCTGTTGAGA GAAAAGGTGA CACCAGCAGC
    ACAGTTTCTG CCCTGTGCTA CACTGTGCCT AAATCAGCTC GGGGCAGCAG TCTTCACGCT
    CTCGAGTCCG GCTCAGACTA CAAGACCCGT GGCATGAGTG GCGACAACAG ACTCATCTTT
    GGTCCAGGAG TGAGCATGTC CACCTGTGAC GTCAAGCTGA TCGATGACAG CGAGTATGAA
    CTCTCTGAGG AGTTTGAGCT GGTGCTGTCA GACGCCTCAG ATAATGCAAG GATGGGCGAC
    GTCACTGTGG CCAAAGTCAT CATCGACGGG CCCAACGACG CCTCTACTGT GTTTCTGGGA
    AACGCATCAT ACACCTTCAG TGAAGATGCA GGCACCATTG AAATCCCTGT TCTGCGGCAG
    GGCAGTGATC TTTCATCAGT CACCTCAGTG TGGTGCGCCA CACGTCCTGC AGAACAAGAT
    TCAGCCACGC CTGGAGTCGA CTACATCCCC AGCTCCAAAA AAGTAGAGTT CAAACCAGGC
    AAGACCGAGG AGACATGCAG TTTGACCATA ATGGATGACA TCCAGAACCC AACAATAGAA
    GGGAAAGAAT CCTTCATTGT GTTTCTTAGC TCTGCTAATG GAGCGGTCCT GACTGAACCC
    TATGAAGCCT CTGTCTACAT CACTGACACC ACTCAAGACA TCCCAAGCAT GCAGTTTGAC
    AAGCTGGCAT ATACAGTAAA GGAAAAAGAT GGCGTCCTGC ACATCCCTAT TTTTAGGACA
    GGAGATCTGT CCTATAAGTC TTCTGTCCGC TGTTTCACCC GCACAATGTC TGCAATGGTT
    ATGGATGACT TTGAGGAGAG GAGAAATGCA GATGATTCGA GAATCACCTT TCAGAAAGGG
    GAGAAGGTGA AGAATTGTAC AGTACTGATC CAGGATGACT CCGTGTTTGA GCCTGAAGAG
    GAGTTCCAGG TCCACCTTGG AAGCCCACAG GGTGACCATT GGATTGGAGC CATGATTGGA
    GTCAACGACA TTGTGACTGT GACCATCACA AATGATGAAG ATGCACCTAC GATCGAGTTT
    GAACAAGCCA CATATCAAGT TCGTGAGCCA CCTGGTCCCG ATGGCATTGA GGTTTTGAAT
    ATCAAGGTAA TTCGTAAAGG AGACCTCGAC CGCACCTCCA AAATTCGATG CAGCACGAGG
    GATGGATCGG CCCAGTCAGG TGTGGACTAT AACCCAAAGA GTCGAGTGCT GAAGTACAGC
    CCTGGTGTGG ACCACATCAT GTTTAAGGTG GAGATTCTGT CCAATGAGGA CAGAGAGTGG
    CATGAGTCTT TCTCTTTGGT CCTTGGTCCT GACGACCCAG TTGAAGCTGT TCTTGGAGAG
    ATCATCATTG CAACAGTCAC TATTCTGGAT CAGGAGGCTG CAGGAAGTCT TATCCTTCCG
    GCTCCACCCA TTGTGGTGTC TCTATCAGAT TATGACTACG TTCAGGAGGT CACTAAAGAG
    GGCAGCAAGA AGTCTCCGTC TCCTGGATAT CCGCTGGTCT GTGTGACCCC CTGTGACCCC
    CACTACCCCA AATACTCCGT CATGAAGGAG CGCTGTGAGG AGGCAGGCAT CAACCAGACT
    TCTCTACACT TCAGCTGGGA GGTGGCAACT CCTACAGACA CCAGCGGAGC AAGATCTCCC
    TTCGAGACTG TGACCGACAA CACACCCTAC ACCACAATCA ACCACATGGT CCTGGACAGC
    ATTTATTTCA GCAGGCGCTT CCATGTGCGC TGTGTAGCCC AAGCCAGAGA TAAAGCCGGA
    CACCTGGGAA CTCCACTGAG GAGCAACATT GTCACCATTG GCACTGAGGG ATCAATATGC
    CACACTCCAG TCACCACAGG GACCGCTCGA GGCTTCCAAG CCCAGTCCTT TATCGCCACT
    CTAAAATACC TGGATGTCAA ACACAAAGAG CATCCAAACA GGATCCACAT TTCAGTTCAG
    ATTCCCCATC AGGATGGGAT GCTTCCACTG GTGTCCACCA TGCCTCTGCA TAACCTGCAC
    TTCCTGCTCT CTGAGTCCAT TTACCGTCAG CAACACGTCT GCTCCAACCT GGTCACACTT
    AAAGACCTGC AGGGTATCTC TGAGACCGGC TTCTTGGATG ACGTGTCATA TGACAGCATA
    TCCCTGGGTC CTGGATATGA CCGGCCATAT CAGTTCAACC CCAATGTTCG AGAACCCAGA
    ACCATACAGC TGTACAAGCA TCTCAATCTG AAGAGCTGCA TCTGGACATT CGATGCTTAT
    TATGACATGA CGGAGCTTAT CGATGTTTGC GGAGGATCAG TAACTGCCGA CTTTCAGGTC
    AGAGACTCGG CTCAGTCCTT CCTGACAGTC CAGGTTCCGC TCTATGTGTC CTACATCTAC
    GTGACGGCTC CTCGTGGATG GGCGTCTCTG GAGCACCACA CAGAAATGGA GTTCTCCTTC
    TTCTACGACA CTGTCTTATG GAGAACAGGC ATTCAGACAG ACAGCGTGCT TTCGGCCAGA
    TTACAGATCA TTCGGATCTT CATTAAGGAT GACGGACGAC TGGTGATTGA GTTTAAAACC
    CACGCCAAGT TCAGAGGTCA GTTTGTCCCA GAGCATCACA CCCTCCCCGG ACAGAAGTCT
    CATCTGATGG CTCCAGATCA CTTAGGAGGC ATGGAGTTCG ACCTACAGCT GGTGTGGAGC
    GCACAGTCTT TTGATTCACC CTATCAGCTC TGGAGAGCCA CAAGCTCCTA CAGCAGGAAA
    GACTATTCAG GGGAGTACAC CGTCTTCCTC ATTCCCTGCA CGGTACAGCC AACACAGGCA
    TGGGTGGACT CTGGGGACAA GCCTCTATCC TGCACAGCAC ATGCACCAGA GAAATTCCTG
    CTGCCCATCG CCTTCCAGCA GACCAACCGC CCTGTCCCTG TTGTCTACTC TCTGAATACT
    GAGTTCCAGC TTTGTAATAA TGAGAAAGTT TTTCTAATGG ATCCTGCTCT CGCAGACATG
    TCTGTGGCTG AGATGGATTA CAAAGGAGCC TTTTCAATGG GTCAAACCCT GTATGGCAGA
    GTCCTGTGGA ATCCTGACCA GAACCTGAAT TCAGCCTACA AGCTGCAGCT GGAGAAAGTG
    TATTTGTGTA CGGGACGGGA TGGATATGTT CCCTTCTTTG ACCCTACAGG CACACTGTAT
    AATGAGGGCC CTCAGTATGG CTGCATTCAA CCAAACAAGC ACCTTAAACA CAGGTTCTTA
    CTTCTGGACC GAAAGCAGCC TGATGTATGT GATCGCTACT TCCATGATGT TCCCTTTGAG
    GCCAGCTTTG CATCTGACAT TCCTGACCTC CTGTCTTTGT CTGCGATGCC CGGTGTAGAC
    GGATTCACAA TGAAGGTTGA TGCCCTTTAT AAGGTTGAAG CAGGGCACCA ATGGTACCTC
    CAGGTCATCT ACGTTATTGG ACCCGAGTCT ATTTCGTCTC CCAGAATCCA GCGCTCAGTG
    ACTTATGAGC TGAAGCGAAG CCGCCGAGAT CTAGTAGACC GATCAGGTCG CCTAATGCTT
    GATGAATCAT TGATCTACGA CAATGAAGGT GACCAGGTAA AGAATGGCAC CAACATGAAG
    ACCTTGAAGC TTGAGATGGA ACCATCAGGC ACCTTCAACC CCCAAATGGG AGGATCCATT
    GGGGGTGGCG TGGCAGCCAT CCTCCTGCTT TTCTTAGTCC TTCTAGCGTT CTGTTTCATG
    GTCAGGAAGT GCCGCAGGGT TGTCAAAAAG AGGCCTTCAA AGACGGTGGA GGATTATCCT
    CTGAACACAA AGGTGGAGGT CTGCATTGAA CGTCTGGAGA AAAACCTCAA CAGCAAGCAC
    TGCTCTGTAA GAAACATCAA CGTTCTCAAC AAGAACCCCG ACACCTACAA AGTCAATGGG
    GTGAAAGTCA AACAGGTCAA TCTGGAGGTC AAACTCCACA ACAATCTAAA TGACGGCACT
    GAAGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.