sorbs2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sorbs2b
Entrez Gene ID	563408
Full Name	sorbin and SH3 domain containing 2b
Synonyms	si:ch211-51g4.4
General protein information	Preferred Names sorbin and SH3 domain containing 2b Names sorbin and SH3 domain-containing protein 2 sorbin and SH3 domain-containing protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017359127.2	XP_017214616.2	sorbin and SH3 domain-containing protein 2 isoform X4
XM_021472804.1	XP_021328479.1	sorbin and SH3 domain-containing protein 2 isoform X5
XM_021472808.1	XP_021328483.1	sorbin and SH3 domain-containing protein 2 isoform X8
XM_021481171.1	XP_021336846.1	sorbin and SH3 domain-containing protein 2 isoform X3
XM_021481176.1	XP_021336851.1	sorbin and SH3 domain-containing protein 2 isoform X8
XM_021481180.1	XP_021336855.1	sorbin and SH3 domain-containing protein 2 isoform X11
XM_021481181.1	XP_021336856.1	sorbin and SH3 domain-containing protein 2 isoform X12
XM_021481187.1	XP_021336862.1	sorbin and SH3 domain-containing protein 2 isoform X18
XM_017359126.1	XP_017214615.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_009291158.2	XP_009289433.1	sorbin and SH3 domain-containing protein 2 isoform X5
XM_009291160.2	XP_009289435.1	sorbin and SH3 domain-containing protein 2 isoform X6
XM_021472800.1	XP_021328475.1	sorbin and SH3 domain-containing protein 2 isoform X3
XM_021472802.1	XP_021328477.1	sorbin and SH3 domain-containing protein 2 isoform X19
XM_021472806.1	XP_021328481.1	sorbin and SH3 domain-containing protein 2 isoform X7
XM_021472814.1	XP_021328489.1	sorbin and SH3 domain-containing protein 2 isoform X15
XM_021481169.1	XP_021336844.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_021481179.1	XP_021336854.1	sorbin and SH3 domain-containing protein 2 isoform X10
XM_021481182.1	XP_021336857.1	sorbin and SH3 domain-containing protein 2 isoform X13
XM_017359129.1	XP_017214618.1	sorbin and SH3 domain-containing protein 2 isoform X4
XM_017359130.1	XP_017214619.1	sorbin and SH3 domain-containing protein 1 isoform X7
XM_021472798.1	XP_021328473.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_021472801.1	XP_021328476.1	sorbin and SH3 domain-containing protein 2 isoform X3
XM_021472805.1	XP_021328480.1	sorbin and SH3 domain-containing protein 2 isoform X6
XM_021472809.1	XP_021328484.1	sorbin and SH3 domain-containing protein 2 isoform X9
XM_021481185.1	XP_021336860.1	sorbin and SH3 domain-containing protein 2 isoform X16
XM_009291157.2	XP_009289432.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_017359127.1	XP_017214616.1	sorbin and SH3 domain-containing protein 2 isoform X2
XM_021472799.1	XP_021328474.1	sorbin and SH3 domain-containing protein 2 isoform X2
XM_021472803.1	XP_021328478.1	sorbin and SH3 domain-containing protein 2 isoform X4
XM_021472807.1	XP_021328482.1	sorbin and SH3 domain-containing protein 2 isoform X8
XM_021472811.1	XP_021328486.1	sorbin and SH3 domain-containing protein 2 isoform X11
XM_021472816.1	XP_021328491.1	sorbin and SH3 domain-containing protein 1 isoform X17
XM_021481172.1	XP_021336847.1	sorbin and SH3 domain-containing protein 2 isoform X4
XM_021481175.1	XP_021336850.1	sorbin and SH3 domain-containing protein 2 isoform X7
XM_021481183.1	XP_021336858.1	sorbin and SH3 domain-containing protein 2 isoform X14
XM_021481184.1	XP_021336859.1	sorbin and SH3 domain-containing protein 2 isoform X15
XM_017359128.1	XP_017214617.1	sorbin and SH3 domain-containing protein 2 isoform X3
XM_009291157.3	XP_009289432.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_021481170.1	XP_021336845.1	sorbin and SH3 domain-containing protein 2 isoform X2
XM_021481173.1	XP_021336848.1	sorbin and SH3 domain-containing protein 2 isoform X5
XM_021481174.1	XP_021336849.1	sorbin and SH3 domain-containing protein 2 isoform X6
XM_021481178.1	XP_021336853.1	sorbin and SH3 domain-containing protein 2 isoform X9
XM_021481186.1	XP_021336861.1	sorbin and SH3 domain-containing protein 1 isoform X17
XM_021472797.1	XP_021328472.1	sorbin and SH3 domain-containing protein 2 isoform X1
XM_021472810.1	XP_021328485.1	sorbin and SH3 domain-containing protein 2 isoform X10
XM_021472812.1	XP_021328487.1	sorbin and SH3 domain-containing protein 2 isoform X13
XM_021472813.1	XP_021328488.1	sorbin and SH3 domain-containing protein 2 isoform X14
XM_021472815.1	XP_021328490.1	sorbin and SH3 domain-containing protein 2 isoform X16
XM_021472817.1	XP_021328492.1	sorbin and SH3 domain-containing protein 2 isoform X18

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SORBS2	XP_001164942.1
Canis lupus familiaris (dog)	SORBS2	XP_005630026.1
Rattus norvegicus (Norway rat)	Sorbs2	NP_446222.1
Mus musculus (house mouse)	Sorbs2	NP_766340.4
Gallus gallus (chicken)	SORBS2	XP_420674.3
Homo sapiens (human)	SORBS2	NP_001257700.1
Danio rerio (zebrafish)	sorbs2b	XP_691857.5
Bos taurus (cattle)	LOC101907303	XP_005226084.1
Xenopus tropicalis (tropical clawed frog)	sorbs2	NP_001135466.1
	SORBS2	XP_001087467.1

Gene Ontology

The following sorbs2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sorbs2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145634	XM_021472815.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa145635	XM_021472816.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa145636	XM_021472817.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa148831	XM_021481187.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa54990	XM_021481186.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa54990	XM_017359130.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa145619	XM_021472798.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145619	XM_021472797.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145620	XM_021472799.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145621	XM_021472801.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145621	XM_021472800.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145622	XM_021472802.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145623	XM_017359127.2 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145623	XM_021472803.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145624	XM_021472804.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145625	XM_021472805.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145626	XM_021472806.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145627	XM_021472807.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145627	XM_021472808.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145628	XM_021472809.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145629	XM_021472810.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145630	XM_021472811.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa145631	XM_021472812.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145632	XM_021472813.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa145633	XM_021472814.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148821	XM_021481170.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148822	XM_021481171.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148823	XM_021481174.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148824	XM_021481176.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148825	XM_021481178.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148826	XM_021481179.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148827	XM_021481180.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148828	XM_021481181.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa148829	XM_021481183.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa148830	XM_021481184.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa54987	XM_017359127.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54987	XM_021481172.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54988	XM_017359128.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54988	XM_021481173.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54989	XM_021481175.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54989	XM_017359129.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa44079	XM_009291157.3 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44079	XM_021481169.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44079	XM_017359126.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44080	XM_009291158.2 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44080	XM_021481182.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44081	XM_009291160.2 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44081	XM_021481185.1 Latest version!	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa44079	XM_009291157.2	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sorbs2b ( XM_021472815.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328490.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.

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CloneID	ODa145634
Clone ID Related Accession (Same CDS sequence)	XM_021472815.1
Accession Version	XM_021472815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAA AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGGAGAA CCAAGTTTTT TGGAACCTTT CCTGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328490.1
CDS	519..4835
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472815.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472815.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAA AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGGAGAA CCAAGTTTTT TGGAACCTTT CCTGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472816.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328491.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.

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CloneID	ODa145635
Clone ID Related Accession (Same CDS sequence)	XM_021472816.1
Accession Version	XM_021472816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG TCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AAAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCGGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TTGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCTACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC ACGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCCC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT CACCTCCGCC 
TCCTTCACTC CTTCGCCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCTCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAAAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGGA GAACCAAGTT TTTTGGAACC 
TTTCCTGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328491.1
CDS	522..4451
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472816.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472816.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG TCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AAAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCGGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TTGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCTACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC ACGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCCC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT CACCTCCGCC 
TCCTTCACTC CTTCGCCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCTCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAAAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGGA GAACCAAGTT TTTTGGAACC 
TTTCCTGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472817.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328492.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa145636
Clone ID Related Accession (Same CDS sequence)	XM_021472817.1
Accession Version	XM_021472817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATCT ACTCCAGCCA TGTGTATAAG GAATTTCCTG CTTTGTTCGA CATAGAAACC 
CTCACCTCTC TTTATCAGCC ATCCACTGAC AGGAGCCTTG AAAGGCCATC AAGCTCTGCA 
AGTGATAACA AGAAGAGGCG AAAATCCGAA CCTGCTACCA CTCAGCCAAG AGCTCACAGC 
AGTGTGGGCA CAACACAAAC ATCTGTCAGA CCACCAGAGC TGCCCAGGGG CAGCAGCACC 
CAGAGGAATC CCCTAACCAG CCCAAGCTCC CCCGCCACCA CTAGAACTAA AGGTGGGGAT 
GTGAGCAATA TGCACACAGC ACATTCAAAC TGTCTAAGTC CAAACCATTC TACCTCAGAA 
CCTGTGTATC AAAACTATGG TGAAAATGAA GACAGTTTTA TGGCTGTCCC AGCTGTCTCA 
AAAGGCTTCT CCAGCCTCCC AGAAGGCTGG AAACCAGCCC AAGAGAATTC AGAAGACTGG 
ATGATCGCAG AGGAGACCTC ACCTAAGCTC AAATCTTGGA GTTGTGATGA CCTCCTGTCT 
GAGGGAAGGG GAAGAGAAGT GCAGACCCGT TGTGAAAGCA CTGGCTCCTT GTTGCACAAT 
GGGGAACTAG AGACATCACA ATACTGTGAT GTCCAAGAGC CTGAGGATCT TAGGGAGAGG 
ACCCGCCACC GCTCTGCCCA CGATGCACCA GGCTTTCTTA AACTCTACAA GAAGATGCAT 
CACATTAACA GGCAAGAGTT GATCAACTCT ACTGTGATCT GCTCTGTCAA ATCCAGGATC 
CGTAAGTATG AGAGCGAACA GCGAAAGGAT AGGCGGACTG GCAATAAGGG CTGCAACGAA 
GAGGTGCCTA GAGACATGGT GCACAACAGG ATTTCTGAAT TTGAGAGTCT GATTCAGAAG 
TCTAAATCCA TGCCAAACCT TGGGGAGGAG TGCCTAATCA GAGGTTCTTC CAGGAGGATC 
AACAGCCCTA AGCGTAGCCT CTCTATTGAG TCGTTGCTAG ATGAAGAACC ACCAGCCAGG 
AATCCCCCTG AAGGACGACC CCAATACCCC AAGATAAACA ACCATGTGCC AATTCACATC 
CATGTCACCA GTGACCAATT ACAAAACTCT GCCATGCAGA ATGATTATTC GGACAGTGAG 
CATGATGCTG TAGTGTCCGA TCTTAGTGAC TTCATCCAGA TTGAGGGGTC ATCTTTTTGC 
AGTGAGAGGG AGTTTGACTT GTGTTCATTT GCCTCCTCCG AAAGTTTCTG TGGATCTGGA 
CACCACCATC ATTTTCACAG GCAACTTGTG AGCTCCTGCA AGGGCCGCTG CCCAGCTTCC 
TATACACGCT TCACCACCAT GATCAAGCAC GAGAGGGCCA AGCAGGATCG GAGGCAACAT 
TTGCGAGTAG AAGATTCTGA GTTTGGTCTC AGTAAGCTTG CATTCCTGGT CAGCCCAGTG 
CCTTTCCGTC GGAAGAACCT GTCACCTTTG AGTTTTTCCC GAATGTCAAA CTCCAAGAGC 
TATGTGTACG AGACTCTAGA TTCAGCTCTA AAAGACATCT ATGATCACAT CCGCACTGAG 
AAACGCAGGA GTAGCCTGTC AGAAAACAGC ATCCTTCATA GGTTGTTGGT GGAGTTGCTT 
CCGGACATTC CTGAGAGGAA TTCCTCCCTG CATGCACTGG GGAGAAACAG TCCTACAATT 
CAACCTCGCT CTTACCACCC ACAACCTGAT GGCATGTCCA GTCAGGACAT TCACCAATCC 
GAGTACTGTC ACCTGTCCCA TAGTGCCTCT CACAACCATA TAGACAGCAA CAACAACCAG 
AGGAACCACA ACTTTAAATA CTGCTATCAA GACCAGGACA CATCCAGAAA TTACACTTAC 
CCTGATGTGG GTCGGCAAAC ACCACAGAGC AGGAGACAAA CCCCTGATGT CAAAGAGAAA 
CTGCCAGCCA GAGCAATATA TGACTTCAAA GCACAGACGG CCAAAGAGCT GACGTTCAAA 
AAAGGAGACA CAGTATACAT CAGTCGGCAG ATAGATAATA ACTGGTATGA AGGAGAATAC 
CATGGACATG TAGGGATATT CCCCATCTCA TATGTGGAGA AAATCCCTCC ATCGGAGCGA 
CATCTGCCAG CAAGACCACC TCCTCCAGCC CAGAGCAAAG AAATTGGAGA AGCAGTTGCC 
CGTTACAACT TTAATGCTGA TACTAATGTG GAACTTTCAT TAAGAAAAGG AGAGCGTGTT 
GTATTGGTGC GGCAGGTGGA CAAAAACTGG TTTGAGGGAA AGATCCCAGG TACAAACAAA 
CAAGGAATAT TTCCAGTGTC GTATGTGGAT GTCATTAAGA AGACTTCAGT ACAAAATACT 
GGACAACCGC CTGGGCCTAG TATACCTACC AGCTTCTCCA GTGACAGACT GAACAGTAGG 
CCGTCATCTA CACGTAACCT CCACAACTCT CCATCCCCAA CTATCCGTCC ACTCTCCTCA 
TCTTCTCCCA GTCCACAGAG AGCTGCACAC CTTCAAGCCA TCACCAGCGA GTGGTTGGCT 
CTCACTCTGG GTCTGTCCCC TTCAGGAACC CCTGCCCCCA CACCTCCTCC CTTCCCTTCC 
AATTTCCAGC CATACTATGA AGCGTTTGAC TCTGGCATCT CGCCATCTCC CGCCTCCTCC 
ACACTGGGCC GCTCTCCAAC ACTCACTCCC CGCGTCTCCA CACCTGCACT CAAAGAGGGC 
CACTTCATTC CCATCTCCTC CCCAAAGTCC TACATGTCCC CTGATCCCAG CCCGTCACCT 
CAACCCTACC TCACCTCCGC CTCCTTCACT CCTTCGCCCA CTTCACCCAC ATTTGACTCC 
AGGTCTGGCA GTGTCATAGA AATTCTGTCC AGTCACAAAG CAGATTTGCC TGTGAAGGAA 
CTGCAGTTGT TCTCAGATCC CAAAGACAGA TACAAGCCAG GCGAGACAGA CTCCCCTAAA 
TTGGGCAGCC CTATTGACTT AGTTGTTTTT GAAGGTCAAA AATCTCCATT GGATATTCTG 
CCACCAAAAG ACCTAGAGGA TGATCTGTGT GAGGAGCTAG TGTCGATTAT AAAGGCCAGC 
CAATCAAAGG GTGCATTCTT AGAAGAGGAG GATTTGTATC ACCAAGAACC AGATACTGTG 
GAAAAGCTAC CCAGACTCTT CATTGAGGAG GAGCAGAATG AAGACAACAG CTTCTTAAAT 
AAAACCCTGC CATCAAATAC ATTCACTCCA CTCCAACTTG CCCATAGTGA GGGTTCAGAT 
ATTGAGCAGG GTGAAGTTTC CCAGAATGCC TCTCCTCGTC TCTCCTCTCT GTCCTCACAG 
ATGTCATTGC CATTCACATC TGCTAAATAC TCCCCCACTT CCCCATGCTC CACCCCACCA 
TTGCCTGGGG TTTCACAGTC ACCCCCTCAC TTGGCAAAAC TGTTCCCTCG GCAGGACCTC 
CGCTCCCCAA AGGTCAAGCC TGTGTTAAGG CGTGATGTTG TGGTTGTTGG TAAACCCCCT 
CGTAGCCCTG TGATGTCTAG GAGGTCCTGC GGATCACCTG TTAGAGGTCA AAACTTTTCG 
CCATCTAATA GGTCCCAAAG ACAAGCATAT GCTTGTGATC CACTTCAAGG TGGAGGGGAA 
CCATTTCAAG CACTGTATAA CTACATGCCA CGGAATGAAG ATGAGCTGGA GCTGAAGGAA 
GGTGATGTTG TTGATGTCAT GGAAAAGTGT GATGATGGAT GGTTTGTGGG AACGTCAAGG 
AGAACCAAGT TTTTTGGAAC CTTTCCTGGG AACTATGTGA AGAGGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328492.1
CDS	385..4155
Translation	MIIYSSHVYKEFPALFDIETLTSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472817.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472817.1

ORF Insert Sequence:

ATGATTATCT ACTCCAGCCA TGTGTATAAG GAATTTCCTG CTTTGTTCGA CATAGAAACC 
CTCACCTCTC TTTATCAGCC ATCCACTGAC AGGAGCCTTG AAAGGCCATC AAGCTCTGCA 
AGTGATAACA AGAAGAGGCG AAAATCCGAA CCTGCTACCA CTCAGCCAAG AGCTCACAGC 
AGTGTGGGCA CAACACAAAC ATCTGTCAGA CCACCAGAGC TGCCCAGGGG CAGCAGCACC 
CAGAGGAATC CCCTAACCAG CCCAAGCTCC CCCGCCACCA CTAGAACTAA AGGTGGGGAT 
GTGAGCAATA TGCACACAGC ACATTCAAAC TGTCTAAGTC CAAACCATTC TACCTCAGAA 
CCTGTGTATC AAAACTATGG TGAAAATGAA GACAGTTTTA TGGCTGTCCC AGCTGTCTCA 
AAAGGCTTCT CCAGCCTCCC AGAAGGCTGG AAACCAGCCC AAGAGAATTC AGAAGACTGG 
ATGATCGCAG AGGAGACCTC ACCTAAGCTC AAATCTTGGA GTTGTGATGA CCTCCTGTCT 
GAGGGAAGGG GAAGAGAAGT GCAGACCCGT TGTGAAAGCA CTGGCTCCTT GTTGCACAAT 
GGGGAACTAG AGACATCACA ATACTGTGAT GTCCAAGAGC CTGAGGATCT TAGGGAGAGG 
ACCCGCCACC GCTCTGCCCA CGATGCACCA GGCTTTCTTA AACTCTACAA GAAGATGCAT 
CACATTAACA GGCAAGAGTT GATCAACTCT ACTGTGATCT GCTCTGTCAA ATCCAGGATC 
CGTAAGTATG AGAGCGAACA GCGAAAGGAT AGGCGGACTG GCAATAAGGG CTGCAACGAA 
GAGGTGCCTA GAGACATGGT GCACAACAGG ATTTCTGAAT TTGAGAGTCT GATTCAGAAG 
TCTAAATCCA TGCCAAACCT TGGGGAGGAG TGCCTAATCA GAGGTTCTTC CAGGAGGATC 
AACAGCCCTA AGCGTAGCCT CTCTATTGAG TCGTTGCTAG ATGAAGAACC ACCAGCCAGG 
AATCCCCCTG AAGGACGACC CCAATACCCC AAGATAAACA ACCATGTGCC AATTCACATC 
CATGTCACCA GTGACCAATT ACAAAACTCT GCCATGCAGA ATGATTATTC GGACAGTGAG 
CATGATGCTG TAGTGTCCGA TCTTAGTGAC TTCATCCAGA TTGAGGGGTC ATCTTTTTGC 
AGTGAGAGGG AGTTTGACTT GTGTTCATTT GCCTCCTCCG AAAGTTTCTG TGGATCTGGA 
CACCACCATC ATTTTCACAG GCAACTTGTG AGCTCCTGCA AGGGCCGCTG CCCAGCTTCC 
TATACACGCT TCACCACCAT GATCAAGCAC GAGAGGGCCA AGCAGGATCG GAGGCAACAT 
TTGCGAGTAG AAGATTCTGA GTTTGGTCTC AGTAAGCTTG CATTCCTGGT CAGCCCAGTG 
CCTTTCCGTC GGAAGAACCT GTCACCTTTG AGTTTTTCCC GAATGTCAAA CTCCAAGAGC 
TATGTGTACG AGACTCTAGA TTCAGCTCTA AAAGACATCT ATGATCACAT CCGCACTGAG 
AAACGCAGGA GTAGCCTGTC AGAAAACAGC ATCCTTCATA GGTTGTTGGT GGAGTTGCTT 
CCGGACATTC CTGAGAGGAA TTCCTCCCTG CATGCACTGG GGAGAAACAG TCCTACAATT 
CAACCTCGCT CTTACCACCC ACAACCTGAT GGCATGTCCA GTCAGGACAT TCACCAATCC 
GAGTACTGTC ACCTGTCCCA TAGTGCCTCT CACAACCATA TAGACAGCAA CAACAACCAG 
AGGAACCACA ACTTTAAATA CTGCTATCAA GACCAGGACA CATCCAGAAA TTACACTTAC 
CCTGATGTGG GTCGGCAAAC ACCACAGAGC AGGAGACAAA CCCCTGATGT CAAAGAGAAA 
CTGCCAGCCA GAGCAATATA TGACTTCAAA GCACAGACGG CCAAAGAGCT GACGTTCAAA 
AAAGGAGACA CAGTATACAT CAGTCGGCAG ATAGATAATA ACTGGTATGA AGGAGAATAC 
CATGGACATG TAGGGATATT CCCCATCTCA TATGTGGAGA AAATCCCTCC ATCGGAGCGA 
CATCTGCCAG CAAGACCACC TCCTCCAGCC CAGAGCAAAG AAATTGGAGA AGCAGTTGCC 
CGTTACAACT TTAATGCTGA TACTAATGTG GAACTTTCAT TAAGAAAAGG AGAGCGTGTT 
GTATTGGTGC GGCAGGTGGA CAAAAACTGG TTTGAGGGAA AGATCCCAGG TACAAACAAA 
CAAGGAATAT TTCCAGTGTC GTATGTGGAT GTCATTAAGA AGACTTCAGT ACAAAATACT 
GGACAACCGC CTGGGCCTAG TATACCTACC AGCTTCTCCA GTGACAGACT GAACAGTAGG 
CCGTCATCTA CACGTAACCT CCACAACTCT CCATCCCCAA CTATCCGTCC ACTCTCCTCA 
TCTTCTCCCA GTCCACAGAG AGCTGCACAC CTTCAAGCCA TCACCAGCGA GTGGTTGGCT 
CTCACTCTGG GTCTGTCCCC TTCAGGAACC CCTGCCCCCA CACCTCCTCC CTTCCCTTCC 
AATTTCCAGC CATACTATGA AGCGTTTGAC TCTGGCATCT CGCCATCTCC CGCCTCCTCC 
ACACTGGGCC GCTCTCCAAC ACTCACTCCC CGCGTCTCCA CACCTGCACT CAAAGAGGGC 
CACTTCATTC CCATCTCCTC CCCAAAGTCC TACATGTCCC CTGATCCCAG CCCGTCACCT 
CAACCCTACC TCACCTCCGC CTCCTTCACT CCTTCGCCCA CTTCACCCAC ATTTGACTCC 
AGGTCTGGCA GTGTCATAGA AATTCTGTCC AGTCACAAAG CAGATTTGCC TGTGAAGGAA 
CTGCAGTTGT TCTCAGATCC CAAAGACAGA TACAAGCCAG GCGAGACAGA CTCCCCTAAA 
TTGGGCAGCC CTATTGACTT AGTTGTTTTT GAAGGTCAAA AATCTCCATT GGATATTCTG 
CCACCAAAAG ACCTAGAGGA TGATCTGTGT GAGGAGCTAG TGTCGATTAT AAAGGCCAGC 
CAATCAAAGG GTGCATTCTT AGAAGAGGAG GATTTGTATC ACCAAGAACC AGATACTGTG 
GAAAAGCTAC CCAGACTCTT CATTGAGGAG GAGCAGAATG AAGACAACAG CTTCTTAAAT 
AAAACCCTGC CATCAAATAC ATTCACTCCA CTCCAACTTG CCCATAGTGA GGGTTCAGAT 
ATTGAGCAGG GTGAAGTTTC CCAGAATGCC TCTCCTCGTC TCTCCTCTCT GTCCTCACAG 
ATGTCATTGC CATTCACATC TGCTAAATAC TCCCCCACTT CCCCATGCTC CACCCCACCA 
TTGCCTGGGG TTTCACAGTC ACCCCCTCAC TTGGCAAAAC TGTTCCCTCG GCAGGACCTC 
CGCTCCCCAA AGGTCAAGCC TGTGTTAAGG CGTGATGTTG TGGTTGTTGG TAAACCCCCT 
CGTAGCCCTG TGATGTCTAG GAGGTCCTGC GGATCACCTG TTAGAGGTCA AAACTTTTCG 
CCATCTAATA GGTCCCAAAG ACAAGCATAT GCTTGTGATC CACTTCAAGG TGGAGGGGAA 
CCATTTCAAG CACTGTATAA CTACATGCCA CGGAATGAAG ATGAGCTGGA GCTGAAGGAA 
GGTGATGTTG TTGATGTCAT GGAAAAGTGT GATGATGGAT GGTTTGTGGG AACGTCAAGG 
AGAACCAAGT TTTTTGGAAC CTTTCCTGGG AACTATGTGA AGAGGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481187.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336862.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.

Price & Availability↑

CloneID	ODa148831
Clone ID Related Accession (Same CDS sequence)	XM_021481187.1
Accession Version	XM_021481187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTATCT ACTCCAGCCA TGTGTATAAG GAATTTCCTG CTTTGTTCGA CATAGAAACC 
CTCACCTCTC TTTATCAGCC ATCCACTGAC AGGAGCTTTG AAAGGCCATC AAGCTCTGCA 
AGTGATAACA AGAAGAGGCG AAAATCCGAA CCTGCTACCA CTCAGCCAAG AGCTCACAGC 
AGTGTGGGCA CAACACAAAC ATCTGTCAGA CCACCAGAGC TGCCCAGGGG CAGCAGCACC 
CAGAGGAATC CCCTCACCAG CCCAAGCTCC CCCGCCACCA CTAGAACTAA AGGTGGGGAT 
GTGAGCAATA TGCACACAGC ACATTCAAAC TGTCTAAGTC CAAACCATTC TACCTCAGAA 
CCTGTGTATC AAAACTATGG TGAAAATGAA GACAGTTTTA TGGCTGTCCC AGCTGTCTCA 
GAAGGCTTCT CCAGCCTCCC AGAAGGCTGG AAACCAGCCC AAGAGAATTC AGAAGACTGG 
ATGATCGCAG AGGAGACCTC ACCTAAGCTC AAATCTTGGA GTTGTGATGA CCTCCTGTCT 
GAGGGAAGGG GAAGAGAAGT GCAGACCCGT TGTGAAAGCA CTGGCTCCTT GTTGCACAAT 
GGGGAACTAG AGACATCACA ATACTGTGAT GTCCAAGAGT CTGAGGATCT TAGGGAGAGG 
ACCCGCCACC GCTCTGCCCA CGATGCACCA GGCTTTCTTA AACTCTACAA GAAGATGCAT 
CACATTAACA GGCAAGAGTT GATAAACTCT ACTGTCATCT GCTCTGTCAA ATCCAGGATC 
CGTAAGTATG AGAGCGAACA GCGAAAGGAT AGGCGAACCG GCAACAAGGG CTGCAACGAA 
GAGGTGCCTA GAGACATGGT GCACAACAGG ATTTCTGAAT TTGAGAGTCT GATTCAGAAG 
TCTAAATCCA TGCCAAACCT TGGGGAGGAG TGCCTAATCA GAGGTTCTTC CAGGAGGATC 
AACAGCCCTA AGCGTAGCCT CTCTATTGAG TCGTTGCTAG ATGAAGAACC ACCAGCCAGG 
AATCCCCCAG AAGGACGGCC CCAATACCCC AAGATAAACA ACCATGTGCC AATTCACATC 
CATGTCACCA GTGACCAATT ACAAAACTCT GCCATGCAGA ATGATTATTC GGACAGTGAG 
CATGATGCTG TAGTGTCCGA TCTTAGTGAC TTCATCCAGA TTGAAGGGTC ATCTTTTTGC 
AGTGAGAGGG AGTTTGACTT GTGTTCATTT GCCTCCTCCG AAAGTTTCTG TGGATCTGGA 
CACCACCATC ATTTTCACAG GCAACTTGTG AGCTCCTGCA AGGGCCGCTG CCCGGCTTCC 
TATACACGCT TCACCACCAT GATCAAGCAT GAGAGGGCCA AGCAGGATCG GAGGCAACAT 
TTGCGAGTAG AAGATTCTGA GTTTGGTCTC AGTAAGCTTG CATTCCTGGT CAGCCCAGTG 
CCTTTCCGTC GGAAGAACCT GTCACCTTTG AGTTTTTCCC GAATGTCAAA CTCCAAGAGC 
TATGTGTACG AGACTCTAGA TTCAGCTCTA AAAGACATCT ATGATCACAT CCGCACTGAG 
AAACGCAGGA GTAGCCTGTC AGAAAACAGC ATCCTTCATA GGTTGTTGGT GGAGTTGCTT 
CCGGACATTC CTGAGAGGAA TTCCTCCCTG CATGCACTGG GGAGAAACAG TCCTACAATT 
CAACCTCGCT CTTACCACCC ACAACCTGAT GGCATGTCCA GTCAGGACAT TCACCAATCC 
GAGTACTGTC ACCTGTCCCA TAGTGCCTCT CACAACCATA TAGACAGCAA CAACAACCAG 
AGGAACCACA ACTTTAAATA CTGCTATCAA GACCAGGACA CATCCAGAAA TTACACTTAC 
CCTGATGTGG GCCGGCAAAC ACCACAGAGC AGGAGACAAA CCCCTGATGT CAGAGAGAAA 
CTGCCAGCCA GAGCAATATA TGACTTCAAA GCACAGACGG CCAAAGAGCT GACGTTCAAA 
AAAGGAGACA CAGTATACAT CAGTCGGCAG ATAGATAATA ACTGGTATGA AGGAGAATAC 
CATGGACATG TAGGGATATT CCCCATCTCA TATGTGGAGA AAATCCCTCC ATCAGAGCGA 
CATCTGCCAG CAAGACCACC TCCTCCAGCC CAGAGCAAAG AAATTGGAGA AGCAGTTGCC 
CGTTACAACT TTAATGCTGA TACTAATGTG GAACTTTCAT TAAGAAAAGG AGAGCGTGTT 
GTATTGGTGC GGCAGGTGGA CAAAAACTGG TTCGAGGGAA AGATCCCAGG TACAAACAAA 
CAAGGAATAT TTCCAGTGTC GTATGTGGAT GTCATTAAGA AGACTTCAGT ACAAAATACT 
GGACAACCGC CTGGGCCTAG TATACCCACC AGCTTCTCCA GTGACAGACT GAACAGTAGG 
CCGTCATCTA CGCGTAACCT CCACAACTCT CCATCCCCAA CTATCCGTCC ACTCTCCTCA 
TCTTCTCCCA GTCCACAGAG AGCTGCACAC CTTCAAGCCA TCACCAGCGA GTGGTTGGCT 
CTCACTCTGG GTCTGTCCCC TTCAGGAACC CCTGCCCCCA CACCTCCTCC CTTCCCTTCC 
AATTTCCAGC CATACTATGA AGCGTTTGAC TCTGGCATCT CGCCATCTCC CGCCTCCTCC 
ACACTGGGCC GCTCTCCAAC ACTCACTCCT CGCGTCTCCA CACCTGCACT CAAAGAGGGC 
CACTTCATTC CCATCTCCTC CCCAAAGTCC TACATGTCCC CTGATCCCAG CCCGTCACCT 
CAACCCTACC TAACCTCCGC CTCCTTCACT CCTTCACCCA CTTCACCCAC ATTTGACTCC 
AGGTCTGGCA GTGTCATAGA AATTCTGTCC AGTCACAAAG CAGATTTGCC TGTGAAGGAA 
CTGCAGTTGT TCTCAGATCC CAAAGACAGA TACAAGCCAG GCGAGACAGA CTCCCCTAAA 
TTGGGCAGCC CTATTGACTT AGTTGTTTTT GAAGGTCAAA AATCTCCATT GGATATTCTG 
CCACCAAAAG ACCTAGAGGA TGATCTGTGT GAGGAGCTAG TGTCGATTAT AAAGGCCAGC 
CAATCAAAGG GTGCATTCTT AGAAGAGGAG GATTTGTATC ACCAAGAACC AGATACTGTG 
GAAAAGCTAC CCAGACTCTT CATTGAGGAG GAGCAGAATG AAGACAACAG CTTCTTAAAT 
AAAACCCTGC CATCAAATAC ATTCACTCCA CTCCAACTTG CCCATAGTGA GGGTTCAGAT 
ATTGAGCAGG GTGAAGTTTC CCAGAATGCC TCTCCTCGTC TCTCCCCTCT GTCCTCACAG 
ATGTCATTGC CATTCACATC TGCTAAATAC TCCCCCACTT CCCCATGCTC CACCCCACCA 
TTGCCTGGGG TTTCACAGTC ACCCCCTCAC TTGGCAAAAC TGTTCCCTCG GCAGGACCTC 
CGCTCCCCAA AGGTCAAGCC TGTGTTAAGG CGTGATGTTG TGGTTGTTGG TAAACCCCCT 
CGTAGCCCTG TGATGTCTAG GAGGTCCTGC GGATCACCTG TTAGAGGTCA AAACTTTTCG 
CCATCTAATA GGTCCCAAAG ACAAGCATAT GCTTGTGATC CACTTCAAGG TGGAGGGGAA 
CCATTTCAAG CACTGTATAA CTACATGCCA CGGAATGAAG ATGAGCTGGA GCTGAAGGAA 
GGTGATGTTG TTGATGTCAT GGAGAAGTGT GATGATGGAT GGTTTGTGGG AACGTCAAGA 
AGAACCAAGT TTTTTGGAAC CTTTCCGGGG AACTATGTGA AGAGGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336862.1
CDS	217..3987
Translation	MIIYSSHVYKEFPALFDIETLTSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481187.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481187.1

ORF Insert Sequence:

ATGATTATCT ACTCCAGCCA TGTGTATAAG GAATTTCCTG CTTTGTTCGA CATAGAAACC 
CTCACCTCTC TTTATCAGCC ATCCACTGAC AGGAGCTTTG AAAGGCCATC AAGCTCTGCA 
AGTGATAACA AGAAGAGGCG AAAATCCGAA CCTGCTACCA CTCAGCCAAG AGCTCACAGC 
AGTGTGGGCA CAACACAAAC ATCTGTCAGA CCACCAGAGC TGCCCAGGGG CAGCAGCACC 
CAGAGGAATC CCCTCACCAG CCCAAGCTCC CCCGCCACCA CTAGAACTAA AGGTGGGGAT 
GTGAGCAATA TGCACACAGC ACATTCAAAC TGTCTAAGTC CAAACCATTC TACCTCAGAA 
CCTGTGTATC AAAACTATGG TGAAAATGAA GACAGTTTTA TGGCTGTCCC AGCTGTCTCA 
GAAGGCTTCT CCAGCCTCCC AGAAGGCTGG AAACCAGCCC AAGAGAATTC AGAAGACTGG 
ATGATCGCAG AGGAGACCTC ACCTAAGCTC AAATCTTGGA GTTGTGATGA CCTCCTGTCT 
GAGGGAAGGG GAAGAGAAGT GCAGACCCGT TGTGAAAGCA CTGGCTCCTT GTTGCACAAT 
GGGGAACTAG AGACATCACA ATACTGTGAT GTCCAAGAGT CTGAGGATCT TAGGGAGAGG 
ACCCGCCACC GCTCTGCCCA CGATGCACCA GGCTTTCTTA AACTCTACAA GAAGATGCAT 
CACATTAACA GGCAAGAGTT GATAAACTCT ACTGTCATCT GCTCTGTCAA ATCCAGGATC 
CGTAAGTATG AGAGCGAACA GCGAAAGGAT AGGCGAACCG GCAACAAGGG CTGCAACGAA 
GAGGTGCCTA GAGACATGGT GCACAACAGG ATTTCTGAAT TTGAGAGTCT GATTCAGAAG 
TCTAAATCCA TGCCAAACCT TGGGGAGGAG TGCCTAATCA GAGGTTCTTC CAGGAGGATC 
AACAGCCCTA AGCGTAGCCT CTCTATTGAG TCGTTGCTAG ATGAAGAACC ACCAGCCAGG 
AATCCCCCAG AAGGACGGCC CCAATACCCC AAGATAAACA ACCATGTGCC AATTCACATC 
CATGTCACCA GTGACCAATT ACAAAACTCT GCCATGCAGA ATGATTATTC GGACAGTGAG 
CATGATGCTG TAGTGTCCGA TCTTAGTGAC TTCATCCAGA TTGAAGGGTC ATCTTTTTGC 
AGTGAGAGGG AGTTTGACTT GTGTTCATTT GCCTCCTCCG AAAGTTTCTG TGGATCTGGA 
CACCACCATC ATTTTCACAG GCAACTTGTG AGCTCCTGCA AGGGCCGCTG CCCGGCTTCC 
TATACACGCT TCACCACCAT GATCAAGCAT GAGAGGGCCA AGCAGGATCG GAGGCAACAT 
TTGCGAGTAG AAGATTCTGA GTTTGGTCTC AGTAAGCTTG CATTCCTGGT CAGCCCAGTG 
CCTTTCCGTC GGAAGAACCT GTCACCTTTG AGTTTTTCCC GAATGTCAAA CTCCAAGAGC 
TATGTGTACG AGACTCTAGA TTCAGCTCTA AAAGACATCT ATGATCACAT CCGCACTGAG 
AAACGCAGGA GTAGCCTGTC AGAAAACAGC ATCCTTCATA GGTTGTTGGT GGAGTTGCTT 
CCGGACATTC CTGAGAGGAA TTCCTCCCTG CATGCACTGG GGAGAAACAG TCCTACAATT 
CAACCTCGCT CTTACCACCC ACAACCTGAT GGCATGTCCA GTCAGGACAT TCACCAATCC 
GAGTACTGTC ACCTGTCCCA TAGTGCCTCT CACAACCATA TAGACAGCAA CAACAACCAG 
AGGAACCACA ACTTTAAATA CTGCTATCAA GACCAGGACA CATCCAGAAA TTACACTTAC 
CCTGATGTGG GCCGGCAAAC ACCACAGAGC AGGAGACAAA CCCCTGATGT CAGAGAGAAA 
CTGCCAGCCA GAGCAATATA TGACTTCAAA GCACAGACGG CCAAAGAGCT GACGTTCAAA 
AAAGGAGACA CAGTATACAT CAGTCGGCAG ATAGATAATA ACTGGTATGA AGGAGAATAC 
CATGGACATG TAGGGATATT CCCCATCTCA TATGTGGAGA AAATCCCTCC ATCAGAGCGA 
CATCTGCCAG CAAGACCACC TCCTCCAGCC CAGAGCAAAG AAATTGGAGA AGCAGTTGCC 
CGTTACAACT TTAATGCTGA TACTAATGTG GAACTTTCAT TAAGAAAAGG AGAGCGTGTT 
GTATTGGTGC GGCAGGTGGA CAAAAACTGG TTCGAGGGAA AGATCCCAGG TACAAACAAA 
CAAGGAATAT TTCCAGTGTC GTATGTGGAT GTCATTAAGA AGACTTCAGT ACAAAATACT 
GGACAACCGC CTGGGCCTAG TATACCCACC AGCTTCTCCA GTGACAGACT GAACAGTAGG 
CCGTCATCTA CGCGTAACCT CCACAACTCT CCATCCCCAA CTATCCGTCC ACTCTCCTCA 
TCTTCTCCCA GTCCACAGAG AGCTGCACAC CTTCAAGCCA TCACCAGCGA GTGGTTGGCT 
CTCACTCTGG GTCTGTCCCC TTCAGGAACC CCTGCCCCCA CACCTCCTCC CTTCCCTTCC 
AATTTCCAGC CATACTATGA AGCGTTTGAC TCTGGCATCT CGCCATCTCC CGCCTCCTCC 
ACACTGGGCC GCTCTCCAAC ACTCACTCCT CGCGTCTCCA CACCTGCACT CAAAGAGGGC 
CACTTCATTC CCATCTCCTC CCCAAAGTCC TACATGTCCC CTGATCCCAG CCCGTCACCT 
CAACCCTACC TAACCTCCGC CTCCTTCACT CCTTCACCCA CTTCACCCAC ATTTGACTCC 
AGGTCTGGCA GTGTCATAGA AATTCTGTCC AGTCACAAAG CAGATTTGCC TGTGAAGGAA 
CTGCAGTTGT TCTCAGATCC CAAAGACAGA TACAAGCCAG GCGAGACAGA CTCCCCTAAA 
TTGGGCAGCC CTATTGACTT AGTTGTTTTT GAAGGTCAAA AATCTCCATT GGATATTCTG 
CCACCAAAAG ACCTAGAGGA TGATCTGTGT GAGGAGCTAG TGTCGATTAT AAAGGCCAGC 
CAATCAAAGG GTGCATTCTT AGAAGAGGAG GATTTGTATC ACCAAGAACC AGATACTGTG 
GAAAAGCTAC CCAGACTCTT CATTGAGGAG GAGCAGAATG AAGACAACAG CTTCTTAAAT 
AAAACCCTGC CATCAAATAC ATTCACTCCA CTCCAACTTG CCCATAGTGA GGGTTCAGAT 
ATTGAGCAGG GTGAAGTTTC CCAGAATGCC TCTCCTCGTC TCTCCCCTCT GTCCTCACAG 
ATGTCATTGC CATTCACATC TGCTAAATAC TCCCCCACTT CCCCATGCTC CACCCCACCA 
TTGCCTGGGG TTTCACAGTC ACCCCCTCAC TTGGCAAAAC TGTTCCCTCG GCAGGACCTC 
CGCTCCCCAA AGGTCAAGCC TGTGTTAAGG CGTGATGTTG TGGTTGTTGG TAAACCCCCT 
CGTAGCCCTG TGATGTCTAG GAGGTCCTGC GGATCACCTG TTAGAGGTCA AAACTTTTCG 
CCATCTAATA GGTCCCAAAG ACAAGCATAT GCTTGTGATC CACTTCAAGG TGGAGGGGAA 
CCATTTCAAG CACTGTATAA CTACATGCCA CGGAATGAAG ATGAGCTGGA GCTGAAGGAA 
GGTGATGTTG TTGATGTCAT GGAGAAGTGT GATGATGGAT GGTTTGTGGG AACGTCAAGA 
AGAACCAAGT TTTTTGGAAC CTTTCCGGGG AACTATGTGA AGAGGCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359130.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214619.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

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CloneID	ODa54990
Clone ID Related Accession (Same CDS sequence)	XM_017359130.1 , XM_021481186.1
Accession Version	XM_017359130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG CCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AGAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCAGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TCGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCCACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC GCGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCTC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT AACCTCCGCC 
TCCTTCACTC CTTCACCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCCCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAGAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGAA GAACCAAGTT TTTTGGAACC 
TTTCCGGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214619.1
CDS	502..4431
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359130.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359130.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG CCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AGAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCAGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TCGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCCACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC GCGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCTC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT AACCTCCGCC 
TCCTTCACTC CTTCACCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCCCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAGAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGAA GAACCAAGTT TTTTGGAACC 
TTTCCGGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481186.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336861.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.

Price & Availability↑

CloneID	ODa54990
Clone ID Related Accession (Same CDS sequence)	XM_017359130.1 , XM_021481186.1
Accession Version	XM_021481186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG CCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AGAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCAGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TCGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCCACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC GCGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCTC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT AACCTCCGCC 
TCCTTCACTC CTTCACCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCCCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAGAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGAA GAACCAAGTT TTTTGGAACC 
TTTCCGGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336861.1
CDS	527..4456
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481186.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481186.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG ACCAGGACAC ATCCAGAAAT TACACTTACC CTGATGTGGG CCGGCAAACA 
CCACAGAGCA GGAGACAAAC CCCTGATGTC AGAGAGAAAC TGCCAGCCAG AGCAATATAT 
GACTTCAAAG CACAGACGGC CAAAGAGCTG ACGTTCAAAA AAGGAGACAC AGTATACATC 
AGTCGGCAGA TAGATAATAA CTGGTATGAA GGAGAATACC ATGGACATGT AGGGATATTC 
CCCATCTCAT ATGTGGAGAA AATCCCTCCA TCAGAGCGAC ATCTGCCAGC AAGACCACCT 
CCTCCAGCCC AGAGCAAAGA AATTGGAGAA GCAGTTGCCC GTTACAACTT TAATGCTGAT 
ACTAATGTGG AACTTTCATT AAGAAAAGGA GAGCGTGTTG TATTGGTGCG GCAGGTGGAC 
AAAAACTGGT TCGAGGGAAA GATCCCAGGT ACAAACAAAC AAGGAATATT TCCAGTGTCG 
TATGTGGATG TCATTAAGAA GACTTCAGTA CAAAATACTG GACAACCGCC TGGGCCTAGT 
ATACCCACCA GCTTCTCCAG TGACAGACTG AACAGTAGGC CGTCATCTAC GCGTAACCTC 
CACAACTCTC CATCCCCAAC TATCCGTCCA CTCTCCTCAT CTTCTCCCAG TCCACAGAGA 
GCTGCACACC TTCAAGCCAT CACCAGCGAG TGGTTGGCTC TCACTCTGGG TCTGTCCCCT 
TCAGGAACCC CTGCCCCCAC ACCTCCTCCC TTCCCTTCCA ATTTCCAGCC ATACTATGAA 
GCGTTTGACT CTGGCATCTC GCCATCTCCC GCCTCCTCCA CACTGGGCCG CTCTCCAACA 
CTCACTCCTC GCGTCTCCAC ACCTGCACTC AAAGAGGGCC ACTTCATTCC CATCTCCTCC 
CCAAAGTCCT ACATGTCCCC TGATCCCAGC CCGTCACCTC AACCCTACCT AACCTCCGCC 
TCCTTCACTC CTTCACCCAC TTCACCCACA TTTGACTCCA GGTCTGGCAG TGTCATAGAA 
ATTCTGTCCA GTCACAAAGC AGATTTGCCT GTGAAGGAAC TGCAGTTGTT CTCAGATCCC 
AAAGACAGAT ACAAGCCAGG CGAGACAGAC TCCCCTAAAT TGGGCAGCCC TATTGACTTA 
GTTGTTTTTG AAGGTCAAAA ATCTCCATTG GATATTCTGC CACCAAAAGA CCTAGAGGAT 
GATCTGTGTG AGGAGCTAGT GTCGATTATA AAGGCCAGCC AATCAAAGGG TGCATTCTTA 
GAAGAGGAGG ATTTGTATCA CCAAGAACCA GATACTGTGG AAAAGCTACC CAGACTCTTC 
ATTGAGGAGG AGCAGAATGA AGACAACAGC TTCTTAAATA AAACCCTGCC ATCAAATACA 
TTCACTCCAC TCCAACTTGC CCATAGTGAG GGTTCAGATA TTGAGCAGGG TGAAGTTTCC 
CAGAATGCCT CTCCTCGTCT CTCCCCTCTG TCCTCACAGA TGTCATTGCC ATTCACATCT 
GCTAAATACT CCCCCACTTC CCCATGCTCC ACCCCACCAT TGCCTGGGGT TTCACAGTCA 
CCCCCTCACT TGGCAAAACT GTTCCCTCGG CAGGACCTCC GCTCCCCAAA GGTCAAGCCT 
GTGTTAAGGC GTGATGTTGT GGTTGTTGGT AAACCCCCTC GTAGCCCTGT GATGTCTAGG 
AGGTCCTGCG GATCACCTGT TAGAGGTCAA AACTTTTCGC CATCTAATAG GTCCCAAAGA 
CAAGCATATG CTTGTGATCC ACTTCAAGGT GGAGGGGAAC CATTTCAAGC ACTGTATAAC 
TACATGCCAC GGAATGAAGA TGAGCTGGAG CTGAAGGAAG GTGATGTTGT TGATGTCATG 
GAGAAGTGTG ATGATGGATG GTTTGTGGGA ACGTCAAGAA GAACCAAGTT TTTTGGAACC 
TTTCCGGGGA ACTATGTGAA GAGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472798.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328473.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa145619
Clone ID Related Accession (Same CDS sequence)	XM_021472798.1 , XM_021472797.1
Accession Version	XM_021472798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AAAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGGAG AACCAAGTTT TTTGGAACCT TTCCTGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328473.1
CDS	482..5950
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472798.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472798.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AAAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGGAG AACCAAGTTT TTTGGAACCT TTCCTGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472797.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328472.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

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CloneID	ODa145619
Clone ID Related Accession (Same CDS sequence)	XM_021472798.1 , XM_021472797.1
Accession Version	XM_021472797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AAAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGGAG AACCAAGTTT TTTGGAACCT TTCCTGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328472.1
CDS	524..5992
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472797.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472797.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AAAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGGAG AACCAAGTTT TTTGGAACCT TTCCTGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472799.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328474.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

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CloneID	ODa145620
Clone ID Related Accession (Same CDS sequence)	XM_021472799.1
Accession Version	XM_021472799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGGGTGAA GTTTCCCAGA ATGCCTCTCC TCGTCTCTCC 
TCTCTGTCCT CACAGATGTC ATTGCCATTC ACATCTGCTA AATACTCCCC CACTTCCCCA 
TGCTCCACCC CACCATTGCC TGGGGTTTCA CAGTCACCCC CTCACTTGGC AAAACTGTTC 
CCTCGGCAGG ACCTCCGCTC CCCAAAGGTC AAGCCTGTGT TAAGGCGTGA TGTTGTGGTT 
GTTGGTAAAC CCCCTCGTAG CCCTGTGATG TCTAGGAGGT CCTGCGGATC ACCTGTTAGA 
GGTCAAAACT TTTCGCCATC TAATAGGTCC CAAAGACAAG CATATGCTTG TGATCCACTT 
CAAGGTGGAG GGGAACCATT TCAAGCACTG TATAACTACA TGCCACGGAA TGAAGATGAG 
CTGGAGCTGA AGGAAGGTGA TGTTGTTGAT GTCATGGAAA AGTGTGATGA TGGATGGTTT 
GTGGGAACGT CAAGGAGAAC CAAGTTTTTT GGAACCTTTC CTGGGAACTA TGTGAAGAGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328474.1
CDS	524..5989
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472799.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472799.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGGGTGAA GTTTCCCAGA ATGCCTCTCC TCGTCTCTCC 
TCTCTGTCCT CACAGATGTC ATTGCCATTC ACATCTGCTA AATACTCCCC CACTTCCCCA 
TGCTCCACCC CACCATTGCC TGGGGTTTCA CAGTCACCCC CTCACTTGGC AAAACTGTTC 
CCTCGGCAGG ACCTCCGCTC CCCAAAGGTC AAGCCTGTGT TAAGGCGTGA TGTTGTGGTT 
GTTGGTAAAC CCCCTCGTAG CCCTGTGATG TCTAGGAGGT CCTGCGGATC ACCTGTTAGA 
GGTCAAAACT TTTCGCCATC TAATAGGTCC CAAAGACAAG CATATGCTTG TGATCCACTT 
CAAGGTGGAG GGGAACCATT TCAAGCACTG TATAACTACA TGCCACGGAA TGAAGATGAG 
CTGGAGCTGA AGGAAGGTGA TGTTGTTGAT GTCATGGAAA AGTGTGATGA TGGATGGTTT 
GTGGGAACGT CAAGGAGAAC CAAGTTTTTT GGAACCTTTC CTGGGAACTA TGTGAAGAGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328475.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X20, mRNA.

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CloneID	ODa145621
Clone ID Related Accession (Same CDS sequence)	XM_021472800.1 , XM_021472801.1
Accession Version	XM_021472800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA TCCTCAGTCT GATGCCCACA 
CTGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGTGGTTCAC ACCAATGCCG TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT TAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGCGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCCTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCC 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTGG ATCCTCCTGC CCGAGCGCCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGACTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCTACAACTA TTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCATGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCCTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TAACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAAAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGCCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT CAACTCTACT 
GTGATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGGACTGGCA ATAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCTGAAG GACGACCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AGGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCACGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGTC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAA AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC GGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTT 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCTACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACAC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCCCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTCA CCTCCGCCTC CTTCACTCCT 
TCGCCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCTCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA AAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGGAGA ACCAAGTTTT TTGGAACCTT TCCTGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328475.1
CDS	622..6039
Translation	MNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472800.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472800.1

ORF Insert Sequence:

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA TCCTCAGTCT GATGCCCACA 
CTGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGTGGTTCAC ACCAATGCCG TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT TAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGCGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCCTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCC 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTGG ATCCTCCTGC CCGAGCGCCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGACTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCTACAACTA TTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCATGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCCTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TAACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAAAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGCCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT CAACTCTACT 
GTGATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGGACTGGCA ATAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCTGAAG GACGACCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AGGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCACGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGTC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAA AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC GGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTT 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCTACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACAC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCCCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTCA CCTCCGCCTC CTTCACTCCT 
TCGCCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCTCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA AAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGGAGA ACCAAGTTTT TTGGAACCTT TCCTGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472801.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328476.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

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CloneID	ODa145621
Clone ID Related Accession (Same CDS sequence)	XM_021472800.1 , XM_021472801.1
Accession Version	XM_021472801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA TCCTCAGTCT GATGCCCACA 
CTGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGTGGTTCAC ACCAATGCCG TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT TAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGCGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCCTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCC 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTGG ATCCTCCTGC CCGAGCGCCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGACTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCTACAACTA TTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCATGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCCTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TAACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAAAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGCCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT CAACTCTACT 
GTGATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGGACTGGCA ATAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCTGAAG GACGACCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AGGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCACGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGTC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAA AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC GGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTT 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCTACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACAC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCCCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTCA CCTCCGCCTC CTTCACTCCT 
TCGCCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCTCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA AAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGGAGA ACCAAGTTTT TTGGAACCTT TCCTGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328476.1
CDS	618..6035
Translation	MNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472801.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472801.1

ORF Insert Sequence:

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA TCCTCAGTCT GATGCCCACA 
CTGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGTGGTTCAC ACCAATGCCG TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT TAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGCGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCCTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCC 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTGG ATCCTCCTGC CCGAGCGCCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGACTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCTACAACTA TTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCATGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCCTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TAACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAAAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGCCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT CAACTCTACT 
GTGATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGGACTGGCA ATAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCTGAAG GACGACCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AGGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCACGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGTC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAA AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC GGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTT 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCTACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACAC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCCCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTCA CCTCCGCCTC CTTCACTCCT 
TCGCCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCTCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA AAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGGAGA ACCAAGTTTT TTGGAACCTT TCCTGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472802.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328477.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X21, mRNA.

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CloneID	ODa145622
Clone ID Related Accession (Same CDS sequence)	XM_021472802.1
Accession Version	XM_021472802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328477.1
CDS	524..5938
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472802.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472802.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359127.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214616.2 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X22, mRNA.

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CloneID	ODa145623
Clone ID Related Accession (Same CDS sequence)	XM_017359127.2 , XM_021472803.1
Accession Version	XM_017359127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017359127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATATCCTC 
AGTCTGATGC CCACACTGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGTGG TTCACACCAA TGCCGTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTTAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGCGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC CTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCCTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTGGATCCT 
CCTGCCCGAG CGCCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGAC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCTAC AACTATTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCAT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCCT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTAACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAAAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GCCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATCAACT CTACTGTGAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGGAC TGGCAATAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC TGAAGGACGA 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAGGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCAGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ACGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGTCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAAAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCGGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTTGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCTA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACACGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CCCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTCACCTCC 
GCCTCCTTCA CTCCTTCGCC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCTCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAAAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GGAGAACCAA GTTTTTTGGA 
ACCTTTCCTG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214616.2
CDS	482..5854
Translation	MPGLCDIKTLQEGEIFNMNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359127.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359127.2

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATATCCTC 
AGTCTGATGC CCACACTGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGTGG TTCACACCAA TGCCGTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTTAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGCGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC CTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCCTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTGGATCCT 
CCTGCCCGAG CGCCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGAC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCTAC AACTATTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCAT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCCT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTAACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAAAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GCCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATCAACT CTACTGTGAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGGAC TGGCAATAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC TGAAGGACGA 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAGGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCAGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ACGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGTCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAAAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCGGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTTGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCTA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACACGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CCCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTCACCTCC 
GCCTCCTTCA CTCCTTCGCC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCTCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAAAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GGAGAACCAA GTTTTTTGGA 
ACCTTTCCTG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472803.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328478.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

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CloneID	ODa145623
Clone ID Related Accession (Same CDS sequence)	XM_017359127.2 , XM_021472803.1
Accession Version	XM_021472803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATATCCTC 
AGTCTGATGC CCACACTGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGTGG TTCACACCAA TGCCGTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTTAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGCGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC CTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCCTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTGGATCCT 
CCTGCCCGAG CGCCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGAC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCTAC AACTATTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCAT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCCT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTAACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAAAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GCCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATCAACT CTACTGTGAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGGAC TGGCAATAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC TGAAGGACGA 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAGGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCAGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ACGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGTCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAAAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCGGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTTGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCTA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACACGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CCCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTCACCTCC 
GCCTCCTTCA CTCCTTCGCC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCTCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAAAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GGAGAACCAA GTTTTTTGGA 
ACCTTTCCTG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328478.1
CDS	524..5896
Translation	MPGLCDIKTLQEGEIFNMNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472803.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472803.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATATCCTC 
AGTCTGATGC CCACACTGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGTGG TTCACACCAA TGCCGTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTTAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGCGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC CTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCCTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTGGATCCT 
CCTGCCCGAG CGCCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGAC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCTAC AACTATTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCAT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCCT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTAACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAAAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GCCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATCAACT CTACTGTGAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGGAC TGGCAATAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC TGAAGGACGA 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAGGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCAGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ACGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGTCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAAAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCGGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTTGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCTA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACACGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CCCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTCACCTCC 
GCCTCCTTCA CTCCTTCGCC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCTCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAAAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GGAGAACCAA GTTTTTTGGA 
ACCTTTCCTG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472804.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328479.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

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CloneID	ODa145624
Clone ID Related Accession (Same CDS sequence)	XM_021472804.1
Accession Version	XM_021472804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328479.1
CDS	524..5878
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472804.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472804.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472805.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328480.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

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CloneID	ODa145625
Clone ID Related Accession (Same CDS sequence)	XM_021472805.1
Accession Version	XM_021472805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG CTTAAACAAG GTGTACTCCC CAGGGCCAGG 
CAAGCGGCCT TTTCCCCACA TTTTCTTCCG AGCAAGGGGA GTACAGTCCT CGGTTTCAGT 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328480.1
CDS	527..5872
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRLKQGVLPRARQAAFSPHFLPSKGSTVLGFST

Target ORF information:

RefSeq Version	XM_021472805.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472805.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG CTTAAACAAG GTGTACTCCC CAGGGCCAGG 
CAAGCGGCCT TTTCCCCACA TTTTCTTCCG AGCAAGGGGA GTACAGTCCT CGGTTTCAGT 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472806.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328481.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

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CloneID	ODa145626
Clone ID Related Accession (Same CDS sequence)	XM_021472806.1
Accession Version	XM_021472806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCCTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TAACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAAAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGCCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT CAACTCTACT GTGATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGGACTGGCA ATAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCTGAAG GACGACCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AGGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCACGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGTC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAA AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC GGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTT GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCTACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACAC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCCCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTCA CCTCCGCCTC CTTCACTCCT TCGCCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCTCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA AAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGGAGA 
ACCAAGTTTT TTGGAACCTT TCCTGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328481.1
CDS	523..5850
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERATSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472806.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472806.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCCTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TAACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAAAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGCCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT CAACTCTACT GTGATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGGACTGGCA ATAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCTGAAG GACGACCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AGGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC AGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCACGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGTC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAA AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC GGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTT GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCTACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACAC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCCCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTCA CCTCCGCCTC CTTCACTCCT TCGCCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCTCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA AAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGGAGA 
ACCAAGTTTT TTGGAACCTT TCCTGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472808.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328483.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X23, mRNA.

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CloneID	ODa145627
Clone ID Related Accession (Same CDS sequence)	XM_021472808.1 , XM_021472807.1
Accession Version	XM_021472808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATATCCTCA GTCTGATGCC CACACTGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGTGGT TCACACCAAT GCCGTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTTAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGCGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCC TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328483.1
CDS	622..5943
Translation	MNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472808.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472808.1

ORF Insert Sequence:

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATATCCTCA GTCTGATGCC CACACTGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGTGGT TCACACCAAT GCCGTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTTAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGCGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCC TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472807.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328482.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.

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CloneID	ODa145627
Clone ID Related Accession (Same CDS sequence)	XM_021472808.1 , XM_021472807.1
Accession Version	XM_021472807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATATCCTCA GTCTGATGCC CACACTGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGTGGT TCACACCAAT GCCGTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTTAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGCGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCC TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328482.1
CDS	618..5939
Translation	MNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472807.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472807.1

ORF Insert Sequence:

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATATCCTCA GTCTGATGCC CACACTGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGTGGT TCACACCAAT GCCGTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTTAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGCGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCC TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472809.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328484.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa145628
Clone ID Related Accession (Same CDS sequence)	XM_021472809.1
Accession Version	XM_021472809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGTCCCAA AGACAAGCAT ATGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328484.1
CDS	526..5775
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRVPKTSICL

Target ORF information:

RefSeq Version	XM_021472809.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472809.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGTCCCAA AGACAAGCAT ATGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472810.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328485.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.

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CloneID	ODa145629
Clone ID Related Accession (Same CDS sequence)	XM_021472810.1
Accession Version	XM_021472810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGC CTGAGGTGCC AGTCCTCCTC AAACAGAGCT ATCCTGGAAC AGCACAGTCC 
CATAACCGGC TTAGACTCTC CCTCGATTTC ATCAGACCTG AGACCTTTTC TCCAGCAGGC 
CCTCACTCCC CCGACCGCTG CTCATCCTGT ACTCAGAGCG AAGCATCCAC TGCAGTGCTG 
GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC GAGGGCGGCT CTCGTACCCC TTCACCAACA 
CTCAGCCAGA TGAGTGTTGT CACAGACAGC ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC 
ATTAATGGCC AGATGACTGT AAACGGTGGT CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC 
ATCCAGAGAC CCTTCTCTCC CTCAACATAC CCTCCTCCTC CTTCACTGAG TCCCAGTATT 
ACAGCAATGC AGCAGCAGGC TAGAACCACT GCCTCCAGCA CTCCAATCTA CGCCAACGTG 
GATCCTCCTG CCCGAGCGCC GCATACTGAC AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC 
TATACTGGCA TCGGTCCTGT GGATGAGTCT GGGATTCCCA TAGCAATACG GACGACTGTT 
GACAGGCCAA AAGATTGGTA CAAGACCATG TTCAAACAGA TTCATGTGGT GCCTAAAACA 
GAGATCGAGT GGTCCGGGTC CCGAACTGCC ACAGATCCTG TTACAAGTCC AGCTACAACT 
ATTTCCAAAC AAGATAAGCA AGCTGCCCCT AATGCTGTCC AGGCTCATCC TGCACCAAAA 
ACCAGCACCT ACCGGCCCAT AAGCAAGAGT GTTTCCGACA ATGGCATATA TGCTTTCAGG 
CTGCCCGTCG CCTCTCCCCT GCCCACATCA ACCTCCACAC AGCAGAGACC CATTGAAAGA 
GAGGCTCCAC AGAGAGGCAG AAGCACACCA GACATGAATG AATGGGGGCC TCCTGATAGA 
AAAGTGGACA CACGGAAATA CAGAGCAGAG CCAAGGAGCA TCTTTGACTA TGAGCCTGGA 
AAATCATCTG TTTTAGAACA GGAGAGAGCG AAAAGTAACT TAAGTCCAGA GGACATAGAT 
TTAGAGAATG AGCCATGGTA TAAGTTCTTT GCTGAACTGG AGTTTGGGCG GCCGCCTCCA 
AAAAAACTTC TGGATTACAA TCCAGAGAGC GCTCCTCGAT TTCCAGTGGA GACCTCTCTT 
TATCAGCCAT CCACTGACAG GAGCCTTGAA AGGCCATCAA GCTCTGCAAG TGATAACAAG 
AAGAGGCGAA AATCCGAACC TGCTACCACT CAGCCAAGAG CTCACAGCAG TGTGGGCACA 
ACACAAACAT CTGTCAGACC ACCAGAGCTG CCCAGGGGCA GCAGCACCCA GAGGAATCCC 
CTAACCAGCC CAAGCTCCCC CGCCACCACT AGAACTAAAG GTGGGGATGT GAGCAATATG 
CACACAGCAC ATTCAAACTG TCTAAGTCCA AACCATTCTA CCTCAGAACC TGTGTATCAA 
AACTATGGTG AAAATGAAGA CAGTTTTATG GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC 
AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA GAGAATTCAG AAGACTGGAT GATCGCAGAG 
GAGACCTCAC CTAAGCTCAA ATCTTGGAGT TGTGATGACC TCCTGTCTGA GGGAAGGGGA 
AGAGAAGTGC AGACCCGTTG TGAAAGCACT GGCTCCTTGT TGCACAATGG GGAACTAGAG 
ACATCACAAT ACTGTGATGT CCAAGAGCCT GAGGATCTTA GGGAGAGGAC CCGCCACCGC 
TCTGCCCACG ATGCACCAGG CTTTCTTAAA CTCTACAAGA AGATGCATCA CATTAACAGG 
CAAGAGTTGA TCAACTCTAC TGTGATCTGC TCTGTCAAAT CCAGGATCCG TAAGTATGAG 
AGCGAACAGC GAAAGGATAG GCGGACTGGC AATAAGGGCT GCAACGAAGA GGTGCCTAGA 
GACATGGTGC ACAACAGGAT TTCTGAATTT GAGAGTCTGA TTCAGAAGTC TAAATCCATG 
CCAAACCTTG GGGAGGAGTG CCTAATCAGA GGTTCTTCCA GGAGGATCAA CAGCCCTAAG 
CGTAGCCTCT CTATTGAGTC GTTGCTAGAT GAAGAACCAC CAGCCAGGAA TCCCCCTGAA 
GGACGACCCC AATACCCCAA GATAAACAAC CATGTGCCAA TTCACATCCA TGTCACCAGT 
GACCAATTAC AAAACTCTGC CATGCAGAAT GATTATTCGG ACAGTGAGCA TGATGCTGTA 
GTGTCCGATC TTAGTGACTT CATCCAGATT GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG 
TTTGACTTGT GTTCATTTGC CTCCTCCGAA AGTTTCTGTG GATCTGGACA CCACCATCAT 
TTTCACAGGC AACTTGTGAG CTCCTGCAAG GGCCGCTGCC CAGCTTCCTA TACACGCTTC 
ACCACCATGA TCAAGCACGA GAGGGCCAAG CAGGATCGGA GGCAACATTT GCGAGTAGAA 
GATTCTGAGT TTGGTCTCAG TAAGCTTGCA TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG 
AAGAACCTGT CACCTTTGAG TTTTTCCCGA ATGTCAAACT CCAAGAGCTA TGTGTACGAG 
ACTCTAGATT CAGCTCTAAA AGACATCTAT GATCACATCC GCACTGAGAA ACGCAGGAGT 
AGCCTGTCAG AAAACAGCAT CCTTCATAGG TTGTTGGTGG AGTTGCTTCC GGACATTCCT 
GAGAGGAATT CCTCCCTGCA TGCACTGGGG AGAAACAGTC CTACAATTCA ACCTCGCTCT 
TACCACCCAC AACCTGATGG CATGTCCAGT CAGGACATTC ACCAATCCGA GTACTGTCAC 
CTGTCCCATA GTGCCTCTCA CAACCATATA GACAGCAACA ACAACCAGAG GAACCACAAC 
TTTAAATACT GCTATCAAGA CCAGGACACA TCCAGAAATT ACACTTACCC TGATGTGGGT 
CGGCAAACAC CACAGAGCAG GAGACAAACC CCTGATGTCA AAGAGAAACT GCCAGCCAGA 
GCAATATATG ACTTCAAAGC ACAGACGGCC AAAGAGCTGA CGTTCAAAAA AGGAGACACA 
GTATACATCA GTCGGCAGAT AGATAATAAC TGGTATGAAG GAGAATACCA TGGACATGTA 
GGGATATTCC CCATCTCATA TGTGGAGAAA ATCCCTCCAT CGGAGCGACA TCTGCCAGCA 
AGACCACCTC CTCCAGCCCA GAGCAAAGAA ATTGGAGAAG CAGTTGCCCG TTACAACTTT 
AATGCTGATA CTAATGTGGA ACTTTCATTA AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG 
CAGGTGGACA AAAACTGGTT TGAGGGAAAG ATCCCAGGTA CAAACAAACA AGGAATATTT 
CCAGTGTCGT ATGTGGATGT CATTAAGAAG ACTTCAGTAC AAAATACTGG ACAACCGCCT 
GGGCCTAGTA TACCTACCAG CTTCTCCAGT GACAGACTGA ACAGTAGGCC GTCATCTACA 
CGTAACCTCC ACAACTCTCC ATCCCCAACT ATCCGTCCAC TCTCCTCATC TTCTCCCAGT 
CCACAGAGAG CTGCACACCT TCAAGCCATC ACCAGCGAGT GGTTGGCTCT CACTCTGGGT 
CTGTCCCCTT CAGGAACCCC TGCCCCCACA CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA 
TACTATGAAG CGTTTGACTC TGGCATCTCG CCATCTCCCG CCTCCTCCAC ACTGGGCCGC 
TCTCCAACAC TCACTCCCCG CGTCTCCACA CCTGCACTCA AAGAGGGCCA CTTCATTCCC 
ATCTCCTCCC CAAAGTCCTA CATGTCCCCT GATCCCAGCC CGTCACCTCA ACCCTACCTC 
ACCTCCGCCT CCTTCACTCC TTCGCCCACT TCACCCACAT TTGACTCCAG GTCTGGCAGT 
GTCATAGAAA TTCTGTCCAG TCACAAAGCA GATTTGCCTG TGAAGGAACT GCAGTTGTTC 
TCAGATCCCA AAGACAGATA CAAGCCAGGC GAGACAGACT CCCCTAAATT GGGCAGCCCT 
ATTGACTTAG TTGTTTTTGA AGGTCAAAAA TCTCCATTGG ATATTCTGCC ACCAAAAGAC 
CTAGAGGATG ATCTGTGTGA GGAGCTAGTG TCGATTATAA AGGCCAGCCA ATCAAAGGGT 
GCATTCTTAG AAGAGGAGGA TTTGTATCAC CAAGAACCAG ATACTGTGGA AAAGCTACCC 
AGACTCTTCA TTGAGGAGGA GCAGAATGAA GACAACAGCT TCTTAAATAA AACCCTGCCA 
TCAAATACAT TCACTCCACT CCAACTTGCC CATAGTGAGG GTTCAGATAT TGAGCAGGGT 
GAAGTTTCCC AGAATGCCTC TCCTCGTCTC TCCTCTCTGT CCTCACAGAT GTCATTGCCA 
TTCACATCTG CTAAATACTC CCCCACTTCC CCATGCTCCA CCCCACCATT GCCTGGGGTT 
TCACAGTCAC CCCCTCACTT GGCAAAACTG TTCCCTCGGC AGGACCTCCG CTCCCCAAAG 
GTCAAGCCTG TGTTAAGGCG TGATGTTGTG GTTGTTGGTA AACCCCCTCG TAGCCCTGTG 
ATGTCTAGGA GGTCCTGCGG ATCACCTGTT AGAGGTCAAA ACTTTTCGCC ATCTAATAGG 
TCCCAAAGAC AAGCATATGC TTGTGATCCA CTTCAAGGTG GAGGGGAACC ATTTCAAGCA 
CTGTATAACT ACATGCCACG GAATGAAGAT GAGCTGGAGC TGAAGGAAGG TGATGTTGTT 
GATGTCATGG AAAAGTGTGA TGATGGATGG TTTGTGGGAA CGTCAAGGAG AACCAAGTTT 
TTTGGAACCT TTCCTGGGAA CTATGTGAAG AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328485.1
CDS	523..5721
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472810.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472810.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGC CTGAGGTGCC AGTCCTCCTC AAACAGAGCT ATCCTGGAAC AGCACAGTCC 
CATAACCGGC TTAGACTCTC CCTCGATTTC ATCAGACCTG AGACCTTTTC TCCAGCAGGC 
CCTCACTCCC CCGACCGCTG CTCATCCTGT ACTCAGAGCG AAGCATCCAC TGCAGTGCTG 
GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC GAGGGCGGCT CTCGTACCCC TTCACCAACA 
CTCAGCCAGA TGAGTGTTGT CACAGACAGC ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC 
ATTAATGGCC AGATGACTGT AAACGGTGGT CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC 
ATCCAGAGAC CCTTCTCTCC CTCAACATAC CCTCCTCCTC CTTCACTGAG TCCCAGTATT 
ACAGCAATGC AGCAGCAGGC TAGAACCACT GCCTCCAGCA CTCCAATCTA CGCCAACGTG 
GATCCTCCTG CCCGAGCGCC GCATACTGAC AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC 
TATACTGGCA TCGGTCCTGT GGATGAGTCT GGGATTCCCA TAGCAATACG GACGACTGTT 
GACAGGCCAA AAGATTGGTA CAAGACCATG TTCAAACAGA TTCATGTGGT GCCTAAAACA 
GAGATCGAGT GGTCCGGGTC CCGAACTGCC ACAGATCCTG TTACAAGTCC AGCTACAACT 
ATTTCCAAAC AAGATAAGCA AGCTGCCCCT AATGCTGTCC AGGCTCATCC TGCACCAAAA 
ACCAGCACCT ACCGGCCCAT AAGCAAGAGT GTTTCCGACA ATGGCATATA TGCTTTCAGG 
CTGCCCGTCG CCTCTCCCCT GCCCACATCA ACCTCCACAC AGCAGAGACC CATTGAAAGA 
GAGGCTCCAC AGAGAGGCAG AAGCACACCA GACATGAATG AATGGGGGCC TCCTGATAGA 
AAAGTGGACA CACGGAAATA CAGAGCAGAG CCAAGGAGCA TCTTTGACTA TGAGCCTGGA 
AAATCATCTG TTTTAGAACA GGAGAGAGCG AAAAGTAACT TAAGTCCAGA GGACATAGAT 
TTAGAGAATG AGCCATGGTA TAAGTTCTTT GCTGAACTGG AGTTTGGGCG GCCGCCTCCA 
AAAAAACTTC TGGATTACAA TCCAGAGAGC GCTCCTCGAT TTCCAGTGGA GACCTCTCTT 
TATCAGCCAT CCACTGACAG GAGCCTTGAA AGGCCATCAA GCTCTGCAAG TGATAACAAG 
AAGAGGCGAA AATCCGAACC TGCTACCACT CAGCCAAGAG CTCACAGCAG TGTGGGCACA 
ACACAAACAT CTGTCAGACC ACCAGAGCTG CCCAGGGGCA GCAGCACCCA GAGGAATCCC 
CTAACCAGCC CAAGCTCCCC CGCCACCACT AGAACTAAAG GTGGGGATGT GAGCAATATG 
CACACAGCAC ATTCAAACTG TCTAAGTCCA AACCATTCTA CCTCAGAACC TGTGTATCAA 
AACTATGGTG AAAATGAAGA CAGTTTTATG GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC 
AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA GAGAATTCAG AAGACTGGAT GATCGCAGAG 
GAGACCTCAC CTAAGCTCAA ATCTTGGAGT TGTGATGACC TCCTGTCTGA GGGAAGGGGA 
AGAGAAGTGC AGACCCGTTG TGAAAGCACT GGCTCCTTGT TGCACAATGG GGAACTAGAG 
ACATCACAAT ACTGTGATGT CCAAGAGCCT GAGGATCTTA GGGAGAGGAC CCGCCACCGC 
TCTGCCCACG ATGCACCAGG CTTTCTTAAA CTCTACAAGA AGATGCATCA CATTAACAGG 
CAAGAGTTGA TCAACTCTAC TGTGATCTGC TCTGTCAAAT CCAGGATCCG TAAGTATGAG 
AGCGAACAGC GAAAGGATAG GCGGACTGGC AATAAGGGCT GCAACGAAGA GGTGCCTAGA 
GACATGGTGC ACAACAGGAT TTCTGAATTT GAGAGTCTGA TTCAGAAGTC TAAATCCATG 
CCAAACCTTG GGGAGGAGTG CCTAATCAGA GGTTCTTCCA GGAGGATCAA CAGCCCTAAG 
CGTAGCCTCT CTATTGAGTC GTTGCTAGAT GAAGAACCAC CAGCCAGGAA TCCCCCTGAA 
GGACGACCCC AATACCCCAA GATAAACAAC CATGTGCCAA TTCACATCCA TGTCACCAGT 
GACCAATTAC AAAACTCTGC CATGCAGAAT GATTATTCGG ACAGTGAGCA TGATGCTGTA 
GTGTCCGATC TTAGTGACTT CATCCAGATT GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG 
TTTGACTTGT GTTCATTTGC CTCCTCCGAA AGTTTCTGTG GATCTGGACA CCACCATCAT 
TTTCACAGGC AACTTGTGAG CTCCTGCAAG GGCCGCTGCC CAGCTTCCTA TACACGCTTC 
ACCACCATGA TCAAGCACGA GAGGGCCAAG CAGGATCGGA GGCAACATTT GCGAGTAGAA 
GATTCTGAGT TTGGTCTCAG TAAGCTTGCA TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG 
AAGAACCTGT CACCTTTGAG TTTTTCCCGA ATGTCAAACT CCAAGAGCTA TGTGTACGAG 
ACTCTAGATT CAGCTCTAAA AGACATCTAT GATCACATCC GCACTGAGAA ACGCAGGAGT 
AGCCTGTCAG AAAACAGCAT CCTTCATAGG TTGTTGGTGG AGTTGCTTCC GGACATTCCT 
GAGAGGAATT CCTCCCTGCA TGCACTGGGG AGAAACAGTC CTACAATTCA ACCTCGCTCT 
TACCACCCAC AACCTGATGG CATGTCCAGT CAGGACATTC ACCAATCCGA GTACTGTCAC 
CTGTCCCATA GTGCCTCTCA CAACCATATA GACAGCAACA ACAACCAGAG GAACCACAAC 
TTTAAATACT GCTATCAAGA CCAGGACACA TCCAGAAATT ACACTTACCC TGATGTGGGT 
CGGCAAACAC CACAGAGCAG GAGACAAACC CCTGATGTCA AAGAGAAACT GCCAGCCAGA 
GCAATATATG ACTTCAAAGC ACAGACGGCC AAAGAGCTGA CGTTCAAAAA AGGAGACACA 
GTATACATCA GTCGGCAGAT AGATAATAAC TGGTATGAAG GAGAATACCA TGGACATGTA 
GGGATATTCC CCATCTCATA TGTGGAGAAA ATCCCTCCAT CGGAGCGACA TCTGCCAGCA 
AGACCACCTC CTCCAGCCCA GAGCAAAGAA ATTGGAGAAG CAGTTGCCCG TTACAACTTT 
AATGCTGATA CTAATGTGGA ACTTTCATTA AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG 
CAGGTGGACA AAAACTGGTT TGAGGGAAAG ATCCCAGGTA CAAACAAACA AGGAATATTT 
CCAGTGTCGT ATGTGGATGT CATTAAGAAG ACTTCAGTAC AAAATACTGG ACAACCGCCT 
GGGCCTAGTA TACCTACCAG CTTCTCCAGT GACAGACTGA ACAGTAGGCC GTCATCTACA 
CGTAACCTCC ACAACTCTCC ATCCCCAACT ATCCGTCCAC TCTCCTCATC TTCTCCCAGT 
CCACAGAGAG CTGCACACCT TCAAGCCATC ACCAGCGAGT GGTTGGCTCT CACTCTGGGT 
CTGTCCCCTT CAGGAACCCC TGCCCCCACA CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA 
TACTATGAAG CGTTTGACTC TGGCATCTCG CCATCTCCCG CCTCCTCCAC ACTGGGCCGC 
TCTCCAACAC TCACTCCCCG CGTCTCCACA CCTGCACTCA AAGAGGGCCA CTTCATTCCC 
ATCTCCTCCC CAAAGTCCTA CATGTCCCCT GATCCCAGCC CGTCACCTCA ACCCTACCTC 
ACCTCCGCCT CCTTCACTCC TTCGCCCACT TCACCCACAT TTGACTCCAG GTCTGGCAGT 
GTCATAGAAA TTCTGTCCAG TCACAAAGCA GATTTGCCTG TGAAGGAACT GCAGTTGTTC 
TCAGATCCCA AAGACAGATA CAAGCCAGGC GAGACAGACT CCCCTAAATT GGGCAGCCCT 
ATTGACTTAG TTGTTTTTGA AGGTCAAAAA TCTCCATTGG ATATTCTGCC ACCAAAAGAC 
CTAGAGGATG ATCTGTGTGA GGAGCTAGTG TCGATTATAA AGGCCAGCCA ATCAAAGGGT 
GCATTCTTAG AAGAGGAGGA TTTGTATCAC CAAGAACCAG ATACTGTGGA AAAGCTACCC 
AGACTCTTCA TTGAGGAGGA GCAGAATGAA GACAACAGCT TCTTAAATAA AACCCTGCCA 
TCAAATACAT TCACTCCACT CCAACTTGCC CATAGTGAGG GTTCAGATAT TGAGCAGGGT 
GAAGTTTCCC AGAATGCCTC TCCTCGTCTC TCCTCTCTGT CCTCACAGAT GTCATTGCCA 
TTCACATCTG CTAAATACTC CCCCACTTCC CCATGCTCCA CCCCACCATT GCCTGGGGTT 
TCACAGTCAC CCCCTCACTT GGCAAAACTG TTCCCTCGGC AGGACCTCCG CTCCCCAAAG 
GTCAAGCCTG TGTTAAGGCG TGATGTTGTG GTTGTTGGTA AACCCCCTCG TAGCCCTGTG 
ATGTCTAGGA GGTCCTGCGG ATCACCTGTT AGAGGTCAAA ACTTTTCGCC ATCTAATAGG 
TCCCAAAGAC AAGCATATGC TTGTGATCCA CTTCAAGGTG GAGGGGAACC ATTTCAAGCA 
CTGTATAACT ACATGCCACG GAATGAAGAT GAGCTGGAGC TGAAGGAAGG TGATGTTGTT 
GATGTCATGG AAAAGTGTGA TGATGGATGG TTTGTGGGAA CGTCAAGGAG AACCAAGTTT 
TTTGGAACCT TTCCTGGGAA CTATGTGAAG AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472811.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328486.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.

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CloneID	ODa145630
Clone ID Related Accession (Same CDS sequence)	XM_021472811.1
Accession Version	XM_021472811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGGTAA AGACAAGCCT GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328486.1
CDS	526..5682
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKVKTSLC

Target ORF information:

RefSeq Version	XM_021472811.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472811.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACA CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCCCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTC ACCTCCGCCT CCTTCACTCC TTCGCCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCTCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGGTAA AGACAAGCCT GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472812.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328487.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.

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CloneID	ODa145631
Clone ID Related Accession (Same CDS sequence)	XM_021472812.1
Accession Version	XM_021472812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328487.1
CDS	521..5422
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472812.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472812.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCCTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTGGATCCTC CTGCCCGAGC GCCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGACT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCTACA 
ACTATTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCATG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCCTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTAACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AAAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG CCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATCAACTC TACTGTGATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGGACT GGCAATAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCT 
GAAGGACGAC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAGGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCAGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA CGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGTCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAAAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCGGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTTGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCTAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACACGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC CCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTCACCTCCG CCTCCTTCAC TCCTTCGCCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCTCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAAAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG GAGAACCAAG 
TTTTTTGGAA CCTTTCCTGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472813.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328488.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.

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CloneID	ODa145632
Clone ID Related Accession (Same CDS sequence)	XM_021472813.1
Accession Version	XM_021472813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGGCCAGAT GACTGTAAAC 
GGTGGTCTGA GTGTGCCAGC AAGTCCTCGT AGTCACATCC AGAGACCCTT CTCTCCCTCA 
ACATACCCTC CTCCTCCTTC ACTGAGTCCC AGTATTACAG CAATGCAGCA GCAGGCTAGA 
ACCACTGCCT CCAGCACTCC AATCTACGCC AACGTGGATC CTCCTGCCCG AGCGCCGCAT 
ACTGACAGGA GAGAAACAGC AGGGAAAGCT CCGCACTATA CTGGCATCGG TCCTGTGGAT 
GAGTCTGGGA TTCCCATAGC AATACGGACG ACTGTTGACA GGCCAAAAGA TTGGTACAAG 
ACCATGTTCA AACAGATTCA TGTGGTGCCT AAAACAGAGA TCGAGTGGTC CGGGTCCCGA 
ACTGCCACAG ATCCTGTTAC AAGTCCAGCT ACAACTATTT CCAAACAAGA TAAGCAAGCT 
GCCCCTAATG CTGTCCAGGC TCATCCTGCA CCAAAAACCA GCACCTACCG GCCCATAAGC 
AAGAGTGTTT CCGACAATGG CATATATGCT TTCAGGCTGC CCGTCGCCTC TCCCCTGCCC 
ACATCAACCT CCACACAGCA GAGACCCATT GAAAGAGAGG CTCCACAGAG AGGCAGAAGC 
ACACCAGACA TGAATGAATG GGGGCCTCCT GATAGAAAAG TGGACACACG GAAATACAGA 
GCAGAGCCAA GGAGCATCTT TGACTATGAG CCTGGAAAAT CATCTGTTTT AGAACAGGAG 
AGAGCGAAAA GTAACTTAAG TCCAGAGGAC ATAGATTTAG AGAATGAGCC ATGGTATAAG 
TTCTTTGCTG AACTGGAGTT TGGGCGGCCG CCTCCAAAAA AACTTCTGGA TTACAATCCA 
GAGAGCGCTC CTCGATTTCC AGTGGAGACC TCTCTTTATC AGCCATCCAC TGACAGGAGC 
CTTGAAAGGC CATCAAGCTC TGCAAGTGAT AACAAGAAGA GGCGAAAATC CGAACCTGCT 
ACCACTCAGC CAAGAGCTCA CAGCAGTGTG GGCACAACAC AAACATCTGT CAGACCACCA 
GAGCTGCCCA GGGGCAGCAG CACCCAGAGG AATCCCCTAA CCAGCCCAAG CTCCCCCGCC 
ACCACTAGAA CTAAAGGTGG GGATGTGAGC AATATGCACA CAGCACATTC AAACTGTCTA 
AGTCCAAACC ATTCTACCTC AGAACCTGTG TATCAAAACT ATGGTGAAAA TGAAGACAGT 
TTTATGGCTG TCCCAGCTGT CTCAAAAGGC TTCTCCAGCC TCCCAGAAGG CTGGAAACCA 
GCCCAAGAGA ATTCAGAAGA CTGGATGATC GCAGAGGAGA CCTCACCTAA GCTCAAATCT 
TGGAGTTGTG ATGACCTCCT GTCTGAGGGA AGGGGAAGAG AAGTGCAGAC CCGTTGTGAA 
AGCACTGGCT CCTTGTTGCA CAATGGGGAA CTAGAGACAT CACAATACTG TGATGTCCAA 
GAGCCTGAGG ATCTTAGGGA GAGGACCCGC CACCGCTCTG CCCACGATGC ACCAGGCTTT 
CTTAAACTCT ACAAGAAGAT GCATCACATT AACAGGCAAG AGTTGATCAA CTCTACTGTG 
ATCTGCTCTG TCAAATCCAG GATCCGTAAG TATGAGAGCG AACAGCGAAA GGATAGGCGG 
ACTGGCAATA AGGGCTGCAA CGAAGAGGTG CCTAGAGACA TGGTGCACAA CAGGATTTCT 
GAATTTGAGA GTCTGATTCA GAAGTCTAAA TCCATGCCAA ACCTTGGGGA GGAGTGCCTA 
ATCAGAGGTT CTTCCAGGAG GATCAACAGC CCTAAGCGTA GCCTCTCTAT TGAGTCGTTG 
CTAGATGAAG AACCACCAGC CAGGAATCCC CCTGAAGGAC GACCCCAATA CCCCAAGATA 
AACAACCATG TGCCAATTCA CATCCATGTC ACCAGTGACC AATTACAAAA CTCTGCCATG 
CAGAATGATT ATTCGGACAG TGAGCATGAT GCTGTAGTGT CCGATCTTAG TGACTTCATC 
CAGATTGAGG GGTCATCTTT TTGCAGTGAG AGGGAGTTTG ACTTGTGTTC ATTTGCCTCC 
TCCGAAAGTT TCTGTGGATC TGGACACCAC CATCATTTTC ACAGGCAACT TGTGAGCTCC 
TGCAAGGGCC GCTGCCCAGC TTCCTATACA CGCTTCACCA CCATGATCAA GCACGAGAGG 
GCCAAGCAGG ATCGGAGGCA ACATTTGCGA GTAGAAGATT CTGAGTTTGG TCTCAGTAAG 
CTTGCATTCC TGGTCAGCCC AGTGCCTTTC CGTCGGAAGA ACCTGTCACC TTTGAGTTTT 
TCCCGAATGT CAAACTCCAA GAGCTATGTG TACGAGACTC TAGATTCAGC TCTAAAAGAC 
ATCTATGATC ACATCCGCAC TGAGAAACGC AGGAGTAGCC TGTCAGAAAA CAGCATCCTT 
CATAGGTTGT TGGTGGAGTT GCTTCCGGAC ATTCCTGAGA GGAATTCCTC CCTGCATGCA 
CTGGGGAGAA ACAGTCCTAC AATTCAACCT CGCTCTTACC ACCCACAACC TGATGGCATG 
TCCAGTCAGG ACATTCACCA ATCCGAGTAC TGTCACCTGT CCCATAGTGC CTCTCACAAC 
CATATAGACA GCAACAACAA CCAGAGGAAC CACAACTTTA AATACTGCTA TCAAGACCAG 
GACACATCCA GAAATTACAC TTACCCTGAT GTGGGTCGGC AAACACCACA GAGCAGGAGA 
CAAACCCCTG ATGTCAAAGA GAAACTGCCA GCCAGAGCAA TATATGACTT CAAAGCACAG 
ACGGCCAAAG AGCTGACGTT CAAAAAAGGA GACACAGTAT ACATCAGTCG GCAGATAGAT 
AATAACTGGT ATGAAGGAGA ATACCATGGA CATGTAGGGA TATTCCCCAT CTCATATGTG 
GAGAAAATCC CTCCATCGGA GCGACATCTG CCAGCAAGAC CACCTCCTCC AGCCCAGAGC 
AAAGAAATTG GAGAAGCAGT TGCCCGTTAC AACTTTAATG CTGATACTAA TGTGGAACTT 
TCATTAAGAA AAGGAGAGCG TGTTGTATTG GTGCGGCAGG TGGACAAAAA CTGGTTTGAG 
GGAAAGATCC CAGGTACAAA CAAACAAGGA ATATTTCCAG TGTCGTATGT GGATGTCATT 
AAGAAGACTT CAGTACAAAA TACTGGACAA CCGCCTGGGC CTAGTATACC TACCAGCTTC 
TCCAGTGACA GACTGAACAG TAGGCCGTCA TCTACACGTA ACCTCCACAA CTCTCCATCC 
CCAACTATCC GTCCACTCTC CTCATCTTCT CCCAGTCCAC AGAGAGCTGC ACACCTTCAA 
GCCATCACCA GCGAGTGGTT GGCTCTCACT CTGGGTCTGT CCCCTTCAGG AACCCCTGCC 
CCCACACCTC CTCCCTTCCC TTCCAATTTC CAGCCATACT ATGAAGCGTT TGACTCTGGC 
ATCTCGCCAT CTCCCGCCTC CTCCACACTG GGCCGCTCTC CAACACTCAC TCCCCGCGTC 
TCCACACCTG CACTCAAAGA GGGCCACTTC ATTCCCATCT CCTCCCCAAA GTCCTACATG 
TCCCCTGATC CCAGCCCGTC ACCTCAACCC TACCTCACCT CCGCCTCCTT CACTCCTTCG 
CCCACTTCAC CCACATTTGA CTCCAGGTCT GGCAGTGTCA TAGAAATTCT GTCCAGTCAC 
AAAGCAGATT TGCCTGTGAA GGAACTGCAG TTGTTCTCAG ATCCCAAAGA CAGATACAAG 
CCAGGCGAGA CAGACTCCCC TAAATTGGGC AGCCCTATTG ACTTAGTTGT TTTTGAAGGT 
CAAAAATCTC CATTGGATAT TCTGCCACCA AAAGACCTAG AGGATGATCT GTGTGAGGAG 
CTAGTGTCGA TTATAAAGGC CAGCCAATCA AAGGGTGCAT TCTTAGAAGA GGAGGATTTG 
TATCACCAAG AACCAGATAC TGTGGAAAAG CTACCCAGAC TCTTCATTGA GGAGGAGCAG 
AATGAAGACA ACAGCTTCTT AAATAAAACC CTGCCATCAA ATACATTCAC TCCACTCCAA 
CTTGCCCATA GTGAGGGTTC AGATATTGAG CAGGGTGAAG TTTCCCAGAA TGCCTCTCCT 
CGTCTCTCCT CTCTGTCCTC ACAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328488.1
CDS	614..5368
Translation	MNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472813.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472813.1

ORF Insert Sequence:

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGGCCAGAT GACTGTAAAC 
GGTGGTCTGA GTGTGCCAGC AAGTCCTCGT AGTCACATCC AGAGACCCTT CTCTCCCTCA 
ACATACCCTC CTCCTCCTTC ACTGAGTCCC AGTATTACAG CAATGCAGCA GCAGGCTAGA 
ACCACTGCCT CCAGCACTCC AATCTACGCC AACGTGGATC CTCCTGCCCG AGCGCCGCAT 
ACTGACAGGA GAGAAACAGC AGGGAAAGCT CCGCACTATA CTGGCATCGG TCCTGTGGAT 
GAGTCTGGGA TTCCCATAGC AATACGGACG ACTGTTGACA GGCCAAAAGA TTGGTACAAG 
ACCATGTTCA AACAGATTCA TGTGGTGCCT AAAACAGAGA TCGAGTGGTC CGGGTCCCGA 
ACTGCCACAG ATCCTGTTAC AAGTCCAGCT ACAACTATTT CCAAACAAGA TAAGCAAGCT 
GCCCCTAATG CTGTCCAGGC TCATCCTGCA CCAAAAACCA GCACCTACCG GCCCATAAGC 
AAGAGTGTTT CCGACAATGG CATATATGCT TTCAGGCTGC CCGTCGCCTC TCCCCTGCCC 
ACATCAACCT CCACACAGCA GAGACCCATT GAAAGAGAGG CTCCACAGAG AGGCAGAAGC 
ACACCAGACA TGAATGAATG GGGGCCTCCT GATAGAAAAG TGGACACACG GAAATACAGA 
GCAGAGCCAA GGAGCATCTT TGACTATGAG CCTGGAAAAT CATCTGTTTT AGAACAGGAG 
AGAGCGAAAA GTAACTTAAG TCCAGAGGAC ATAGATTTAG AGAATGAGCC ATGGTATAAG 
TTCTTTGCTG AACTGGAGTT TGGGCGGCCG CCTCCAAAAA AACTTCTGGA TTACAATCCA 
GAGAGCGCTC CTCGATTTCC AGTGGAGACC TCTCTTTATC AGCCATCCAC TGACAGGAGC 
CTTGAAAGGC CATCAAGCTC TGCAAGTGAT AACAAGAAGA GGCGAAAATC CGAACCTGCT 
ACCACTCAGC CAAGAGCTCA CAGCAGTGTG GGCACAACAC AAACATCTGT CAGACCACCA 
GAGCTGCCCA GGGGCAGCAG CACCCAGAGG AATCCCCTAA CCAGCCCAAG CTCCCCCGCC 
ACCACTAGAA CTAAAGGTGG GGATGTGAGC AATATGCACA CAGCACATTC AAACTGTCTA 
AGTCCAAACC ATTCTACCTC AGAACCTGTG TATCAAAACT ATGGTGAAAA TGAAGACAGT 
TTTATGGCTG TCCCAGCTGT CTCAAAAGGC TTCTCCAGCC TCCCAGAAGG CTGGAAACCA 
GCCCAAGAGA ATTCAGAAGA CTGGATGATC GCAGAGGAGA CCTCACCTAA GCTCAAATCT 
TGGAGTTGTG ATGACCTCCT GTCTGAGGGA AGGGGAAGAG AAGTGCAGAC CCGTTGTGAA 
AGCACTGGCT CCTTGTTGCA CAATGGGGAA CTAGAGACAT CACAATACTG TGATGTCCAA 
GAGCCTGAGG ATCTTAGGGA GAGGACCCGC CACCGCTCTG CCCACGATGC ACCAGGCTTT 
CTTAAACTCT ACAAGAAGAT GCATCACATT AACAGGCAAG AGTTGATCAA CTCTACTGTG 
ATCTGCTCTG TCAAATCCAG GATCCGTAAG TATGAGAGCG AACAGCGAAA GGATAGGCGG 
ACTGGCAATA AGGGCTGCAA CGAAGAGGTG CCTAGAGACA TGGTGCACAA CAGGATTTCT 
GAATTTGAGA GTCTGATTCA GAAGTCTAAA TCCATGCCAA ACCTTGGGGA GGAGTGCCTA 
ATCAGAGGTT CTTCCAGGAG GATCAACAGC CCTAAGCGTA GCCTCTCTAT TGAGTCGTTG 
CTAGATGAAG AACCACCAGC CAGGAATCCC CCTGAAGGAC GACCCCAATA CCCCAAGATA 
AACAACCATG TGCCAATTCA CATCCATGTC ACCAGTGACC AATTACAAAA CTCTGCCATG 
CAGAATGATT ATTCGGACAG TGAGCATGAT GCTGTAGTGT CCGATCTTAG TGACTTCATC 
CAGATTGAGG GGTCATCTTT TTGCAGTGAG AGGGAGTTTG ACTTGTGTTC ATTTGCCTCC 
TCCGAAAGTT TCTGTGGATC TGGACACCAC CATCATTTTC ACAGGCAACT TGTGAGCTCC 
TGCAAGGGCC GCTGCCCAGC TTCCTATACA CGCTTCACCA CCATGATCAA GCACGAGAGG 
GCCAAGCAGG ATCGGAGGCA ACATTTGCGA GTAGAAGATT CTGAGTTTGG TCTCAGTAAG 
CTTGCATTCC TGGTCAGCCC AGTGCCTTTC CGTCGGAAGA ACCTGTCACC TTTGAGTTTT 
TCCCGAATGT CAAACTCCAA GAGCTATGTG TACGAGACTC TAGATTCAGC TCTAAAAGAC 
ATCTATGATC ACATCCGCAC TGAGAAACGC AGGAGTAGCC TGTCAGAAAA CAGCATCCTT 
CATAGGTTGT TGGTGGAGTT GCTTCCGGAC ATTCCTGAGA GGAATTCCTC CCTGCATGCA 
CTGGGGAGAA ACAGTCCTAC AATTCAACCT CGCTCTTACC ACCCACAACC TGATGGCATG 
TCCAGTCAGG ACATTCACCA ATCCGAGTAC TGTCACCTGT CCCATAGTGC CTCTCACAAC 
CATATAGACA GCAACAACAA CCAGAGGAAC CACAACTTTA AATACTGCTA TCAAGACCAG 
GACACATCCA GAAATTACAC TTACCCTGAT GTGGGTCGGC AAACACCACA GAGCAGGAGA 
CAAACCCCTG ATGTCAAAGA GAAACTGCCA GCCAGAGCAA TATATGACTT CAAAGCACAG 
ACGGCCAAAG AGCTGACGTT CAAAAAAGGA GACACAGTAT ACATCAGTCG GCAGATAGAT 
AATAACTGGT ATGAAGGAGA ATACCATGGA CATGTAGGGA TATTCCCCAT CTCATATGTG 
GAGAAAATCC CTCCATCGGA GCGACATCTG CCAGCAAGAC CACCTCCTCC AGCCCAGAGC 
AAAGAAATTG GAGAAGCAGT TGCCCGTTAC AACTTTAATG CTGATACTAA TGTGGAACTT 
TCATTAAGAA AAGGAGAGCG TGTTGTATTG GTGCGGCAGG TGGACAAAAA CTGGTTTGAG 
GGAAAGATCC CAGGTACAAA CAAACAAGGA ATATTTCCAG TGTCGTATGT GGATGTCATT 
AAGAAGACTT CAGTACAAAA TACTGGACAA CCGCCTGGGC CTAGTATACC TACCAGCTTC 
TCCAGTGACA GACTGAACAG TAGGCCGTCA TCTACACGTA ACCTCCACAA CTCTCCATCC 
CCAACTATCC GTCCACTCTC CTCATCTTCT CCCAGTCCAC AGAGAGCTGC ACACCTTCAA 
GCCATCACCA GCGAGTGGTT GGCTCTCACT CTGGGTCTGT CCCCTTCAGG AACCCCTGCC 
CCCACACCTC CTCCCTTCCC TTCCAATTTC CAGCCATACT ATGAAGCGTT TGACTCTGGC 
ATCTCGCCAT CTCCCGCCTC CTCCACACTG GGCCGCTCTC CAACACTCAC TCCCCGCGTC 
TCCACACCTG CACTCAAAGA GGGCCACTTC ATTCCCATCT CCTCCCCAAA GTCCTACATG 
TCCCCTGATC CCAGCCCGTC ACCTCAACCC TACCTCACCT CCGCCTCCTT CACTCCTTCG 
CCCACTTCAC CCACATTTGA CTCCAGGTCT GGCAGTGTCA TAGAAATTCT GTCCAGTCAC 
AAAGCAGATT TGCCTGTGAA GGAACTGCAG TTGTTCTCAG ATCCCAAAGA CAGATACAAG 
CCAGGCGAGA CAGACTCCCC TAAATTGGGC AGCCCTATTG ACTTAGTTGT TTTTGAAGGT 
CAAAAATCTC CATTGGATAT TCTGCCACCA AAAGACCTAG AGGATGATCT GTGTGAGGAG 
CTAGTGTCGA TTATAAAGGC CAGCCAATCA AAGGGTGCAT TCTTAGAAGA GGAGGATTTG 
TATCACCAAG AACCAGATAC TGTGGAAAAG CTACCCAGAC TCTTCATTGA GGAGGAGCAG 
AATGAAGACA ACAGCTTCTT AAATAAAACC CTGCCATCAA ATACATTCAC TCCACTCCAA 
CTTGCCCATA GTGAGGGTTC AGATATTGAG CAGGGTGAAG TTTCCCAGAA TGCCTCTCCT 
CGTCTCTCCT CTCTGTCCTC ACAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAAAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGGAGAACC AAGTTTTTTG GAACCTTTCC TGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021472814.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328489.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.

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CloneID	ODa145633
Clone ID Related Accession (Same CDS sequence)	XM_021472814.1
Accession Version	XM_021472814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394913.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCTCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAAAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GGAGAACCAA GTTTTTTGGA ACCTTTCCTG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328489.1
CDS	522..5144
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHILSLMPTLDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCRPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRAFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSPAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTTVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTISKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSLERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSKGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQEPEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVKEKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRQGEVSQNASPRLSSLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021472814.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472814.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ATCCTCAGTC TGATGCCCAC ACTGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGTGGTTCA 
CACCAATGCC GTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TTAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGCGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCCTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC CTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTG GATCCTCCTG CCCGAGCGCC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGACTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCTACAACT ATTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCATGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCCTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTAACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAAA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGCCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TCAACTCTAC TGTGATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGGACTGGC 
AATAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCTGAA GGACGACCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAGGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CAGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCACGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGT CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA AAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CGGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT TGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCTACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCTCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAAAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GGAGAACCAA GTTTTTTGGA ACCTTTCCTG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481170.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336845.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

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CloneID	ODa148821
Clone ID Related Accession (Same CDS sequence)	XM_021481170.1
Accession Version	XM_021481170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGGGTGAA GTTTCCCAGA ATGCCTCTCC TCGTCTCTCC 
CCTCTGTCCT CACAGATGTC ATTGCCATTC ACATCTGCTA AATACTCCCC CACTTCCCCA 
TGCTCCACCC CACCATTGCC TGGGGTTTCA CAGTCACCCC CTCACTTGGC AAAACTGTTC 
CCTCGGCAGG ACCTCCGCTC CCCAAAGGTC AAGCCTGTGT TAAGGCGTGA TGTTGTGGTT 
GTTGGTAAAC CCCCTCGTAG CCCTGTGATG TCTAGGAGGT CCTGCGGATC ACCTGTTAGA 
GGTCAAAACT TTTCGCCATC TAATAGGTCC CAAAGACAAG CATATGCTTG TGATCCACTT 
CAAGGTGGAG GGGAACCATT TCAAGCACTG TATAACTACA TGCCACGGAA TGAAGATGAG 
CTGGAGCTGA AGGAAGGTGA TGTTGTTGAT GTCATGGAGA AGTGTGATGA TGGATGGTTT 
GTGGGAACGT CAAGAAGAAC CAAGTTTTTT GGAACCTTTC CGGGGAACTA TGTGAAGAGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336845.1
CDS	527..5992
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481170.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481170.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGGGTGAA GTTTCCCAGA ATGCCTCTCC TCGTCTCTCC 
CCTCTGTCCT CACAGATGTC ATTGCCATTC ACATCTGCTA AATACTCCCC CACTTCCCCA 
TGCTCCACCC CACCATTGCC TGGGGTTTCA CAGTCACCCC CTCACTTGGC AAAACTGTTC 
CCTCGGCAGG ACCTCCGCTC CCCAAAGGTC AAGCCTGTGT TAAGGCGTGA TGTTGTGGTT 
GTTGGTAAAC CCCCTCGTAG CCCTGTGATG TCTAGGAGGT CCTGCGGATC ACCTGTTAGA 
GGTCAAAACT TTTCGCCATC TAATAGGTCC CAAAGACAAG CATATGCTTG TGATCCACTT 
CAAGGTGGAG GGGAACCATT TCAAGCACTG TATAACTACA TGCCACGGAA TGAAGATGAG 
CTGGAGCTGA AGGAAGGTGA TGTTGTTGAT GTCATGGAGA AGTGTGATGA TGGATGGTTT 
GTGGGAACGT CAAGAAGAAC CAAGTTTTTT GGAACCTTTC CGGGGAACTA TGTGAAGAGG 
CTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481171.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336846.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

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CloneID	ODa148822
Clone ID Related Accession (Same CDS sequence)	XM_021481171.1
Accession Version	XM_021481171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA CCCTCAGTCT GATGCCCACA 
CAGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGCGGTTCAC ACCAATGCCC TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT CAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGGGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCGTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCG 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTAG ATCCTCCTGC CCGAGCACCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGTCTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCCACAACTA CTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCGTGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCTTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TCACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAGAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGTCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT AAACTCTACT 
GTCATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGAACCGGCA ACAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCAGAAG GACGGCCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AAGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCATGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGCC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAG AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC AGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTC 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCCACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACGC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCTCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTAA CCTCCGCCTC CTTCACTCCT 
TCACCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCCCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA GAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGAAGA ACCAAGTTTT TTGGAACCTT TCCGGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336846.1
CDS	344..5761
Translation	MNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481171.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481171.1

ORF Insert Sequence:

ATGAACACAG ACAGTGGAGG ACAAATCCGC AAATGTGCCA CGCTTTCACT CACTTTAACC 
CCCATGAAGA GAATCCAGAG CTCGCCAAAC CTATACTCAC TCACAGACTC TGAATCACAA 
TCCAGAGATT CAGATTTGTG GAGGCCCAGT AGTAGCACTG ATGGTCTAAG AAACGGTGAC 
GTCTGCTCTT CCTCTCTGGC AGCTAAAGGT TACAGAAGTG TCAGGCCCAT CTTTCAGGAA 
AAAAAGTCTC CCACACCGGC ACAAGAGCAA AATGTCCATA CCCTCAGTCT GATGCCCACA 
CAGGACCAAA TTTCAGGCTT TTCACAGCAG TCACACTTGC AACCTCCGTC AAGGGAGCTG 
GAGAGACACG TAGCTTCCCT CTCCATCCAC ATTCACCCGA GGCCTCAGAT ATCGGGACCC 
CGCACACAGG CAATGTCTTT GAGACCTCCC ACCCCTCCTA AAAGGATGGA CCGGCAGGAT 
CGCGGTTCAC ACCAATGCCC TCCACTGCCA TCTGCAGCCC CGCCAGTGCC TGAGGTGCCA 
GTCCTCCTCA AACAGAGCTA TCCTGGAACA GCACAGTCCC ATAACCGGCT CAGACTCTCC 
CTCGATTTCA TCAGACCTGA GACCTTTTCT CCAGCAGGCC CTCACTCCCC CGACCGCTGC 
TCATCCTGTA CTCAGAGCGA AGCATCCACT GCAGTGCTGG AGGAGTTGAG GGGGTTTGGA 
CTGGGCCCCG AGGGCGGCTC TCGTACCCCT TCACCAACAC TCAGCCAGAT GAGTGTTGTC 
ACAGACAGCA TGGGGTTACA CTCCCGTGCA GCTAGCAACA TTAATGGCCA GATGACTGTA 
AACGGTGGTC TGAGTGTGCC AGCAAGTCCT CGTAGTCACA TCCAGAGACC CTTCTCTCCG 
TCAACATACC CTCCTCCTCC TTCACTGAGT CCCAGTATTA CAGCAATGCA GCAGCAGGCT 
AGAACCACTG CCTCCAGCAC TCCAATCTAC GCCAACGTAG ATCCTCCTGC CCGAGCACCG 
CATACTGACA GGAGAGAAAC AGCAGGGAAA GCTCCGCACT ATACTGGCAT CGGTCCTGTG 
GATGAGTCTG GGATTCCCAT AGCAATACGG ACGTCTGTTG ACAGGCCAAA AGATTGGTAC 
AAGACCATGT TCAAACAGAT TCATGTGGTG CCTAAAACAG AGATCGAGTG GTCCGGGTCC 
CGAACTGCCA CAGATCCTGT TACAAGTCCA GCCACAACTA CTTCCAAACA AGATAAGCAA 
GCTGCCCCTA ATGCTGTCCA GGCTCATCCT GCACCAAAAA CCAGCACCTA CCGGCCCATA 
AGCAAGAGTG TTTCCGACAA TGGCATATAT GCTTTCAGGC TGCCCGTCGC CTCTCCCCTG 
CCCACATCAA CCTCCACACA GCAGAGACCC ATTGAAAGAG AGGCTCCACA GAGAGGCAGA 
AGCACACCAG ACATGAATGA ATGGGGGCCT CCTGATAGAA AAGTGGACAC ACGGAAATAC 
AGAGCAGAGC CAAGGAGCAT CTTTGACTAT GAGCCTGGAA AATCATCTGT TTTAGAACAG 
GAGAGAGCGA AAAGTAACTT AAGTCCAGAG GACATAGATT TAGAGAATGA GCCGTGGTAT 
AAGTTCTTTG CTGAACTGGA GTTTGGGCGG CCGCCTCCAA AAAAACTTCT GGATTACAAT 
CCAGAGAGCG CTCCTCGATT TCCAGTGGAG ACCTCTCTTT ATCAGCCATC CACTGACAGG 
AGCTTTGAAA GGCCATCAAG CTCTGCAAGT GATAACAAGA AGAGGCGAAA ATCCGAACCT 
GCTACCACTC AGCCAAGAGC TCACAGCAGT GTGGGCACAA CACAAACATC TGTCAGACCA 
CCAGAGCTGC CCAGGGGCAG CAGCACCCAG AGGAATCCCC TCACCAGCCC AAGCTCCCCC 
GCCACCACTA GAACTAAAGG TGGGGATGTG AGCAATATGC ACACAGCACA TTCAAACTGT 
CTAAGTCCAA ACCATTCTAC CTCAGAACCT GTGTATCAAA ACTATGGTGA AAATGAAGAC 
AGTTTTATGG CTGTCCCAGC TGTCTCAGAA GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA 
CCAGCCCAAG AGAATTCAGA AGACTGGATG ATCGCAGAGG AGACCTCACC TAAGCTCAAA 
TCTTGGAGTT GTGATGACCT CCTGTCTGAG GGAAGGGGAA GAGAAGTGCA GACCCGTTGT 
GAAAGCACTG GCTCCTTGTT GCACAATGGG GAACTAGAGA CATCACAATA CTGTGATGTC 
CAAGAGTCTG AGGATCTTAG GGAGAGGACC CGCCACCGCT CTGCCCACGA TGCACCAGGC 
TTTCTTAAAC TCTACAAGAA GATGCATCAC ATTAACAGGC AAGAGTTGAT AAACTCTACT 
GTCATCTGCT CTGTCAAATC CAGGATCCGT AAGTATGAGA GCGAACAGCG AAAGGATAGG 
CGAACCGGCA ACAAGGGCTG CAACGAAGAG GTGCCTAGAG ACATGGTGCA CAACAGGATT 
TCTGAATTTG AGAGTCTGAT TCAGAAGTCT AAATCCATGC CAAACCTTGG GGAGGAGTGC 
CTAATCAGAG GTTCTTCCAG GAGGATCAAC AGCCCTAAGC GTAGCCTCTC TATTGAGTCG 
TTGCTAGATG AAGAACCACC AGCCAGGAAT CCCCCAGAAG GACGGCCCCA ATACCCCAAG 
ATAAACAACC ATGTGCCAAT TCACATCCAT GTCACCAGTG ACCAATTACA AAACTCTGCC 
ATGCAGAATG ATTATTCGGA CAGTGAGCAT GATGCTGTAG TGTCCGATCT TAGTGACTTC 
ATCCAGATTG AAGGGTCATC TTTTTGCAGT GAGAGGGAGT TTGACTTGTG TTCATTTGCC 
TCCTCCGAAA GTTTCTGTGG ATCTGGACAC CACCATCATT TTCACAGGCA ACTTGTGAGC 
TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT ACACGCTTCA CCACCATGAT CAAGCATGAG 
AGGGCCAAGC AGGATCGGAG GCAACATTTG CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT 
AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT TTCCGTCGGA AGAACCTGTC ACCTTTGAGT 
TTTTCCCGAA TGTCAAACTC CAAGAGCTAT GTGTACGAGA CTCTAGATTC AGCTCTAAAA 
GACATCTATG ATCACATCCG CACTGAGAAA CGCAGGAGTA GCCTGTCAGA AAACAGCATC 
CTTCATAGGT TGTTGGTGGA GTTGCTTCCG GACATTCCTG AGAGGAATTC CTCCCTGCAT 
GCACTGGGGA GAAACAGTCC TACAATTCAA CCTCGCTCTT ACCACCCACA ACCTGATGGC 
ATGTCCAGTC AGGACATTCA CCAATCCGAG TACTGTCACC TGTCCCATAG TGCCTCTCAC 
AACCATATAG ACAGCAACAA CAACCAGAGG AACCACAACT TTAAATACTG CTATCAAGAC 
CAGGACACAT CCAGAAATTA CACTTACCCT GATGTGGGCC GGCAAACACC ACAGAGCAGG 
AGACAAACCC CTGATGTCAG AGAGAAACTG CCAGCCAGAG CAATATATGA CTTCAAAGCA 
CAGACGGCCA AAGAGCTGAC GTTCAAAAAA GGAGACACAG TATACATCAG TCGGCAGATA 
GATAATAACT GGTATGAAGG AGAATACCAT GGACATGTAG GGATATTCCC CATCTCATAT 
GTGGAGAAAA TCCCTCCATC AGAGCGACAT CTGCCAGCAA GACCACCTCC TCCAGCCCAG 
AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT TACAACTTTA ATGCTGATAC TAATGTGGAA 
CTTTCATTAA GAAAAGGAGA GCGTGTTGTA TTGGTGCGGC AGGTGGACAA AAACTGGTTC 
GAGGGAAAGA TCCCAGGTAC AAACAAACAA GGAATATTTC CAGTGTCGTA TGTGGATGTC 
ATTAAGAAGA CTTCAGTACA AAATACTGGA CAACCGCCTG GGCCTAGTAT ACCCACCAGC 
TTCTCCAGTG ACAGACTGAA CAGTAGGCCG TCATCTACGC GTAACCTCCA CAACTCTCCA 
TCCCCAACTA TCCGTCCACT CTCCTCATCT TCTCCCAGTC CACAGAGAGC TGCACACCTT 
CAAGCCATCA CCAGCGAGTG GTTGGCTCTC ACTCTGGGTC TGTCCCCTTC AGGAACCCCT 
GCCCCCACAC CTCCTCCCTT CCCTTCCAAT TTCCAGCCAT ACTATGAAGC GTTTGACTCT 
GGCATCTCGC CATCTCCCGC CTCCTCCACA CTGGGCCGCT CTCCAACACT CACTCCTCGC 
GTCTCCACAC CTGCACTCAA AGAGGGCCAC TTCATTCCCA TCTCCTCCCC AAAGTCCTAC 
ATGTCCCCTG ATCCCAGCCC GTCACCTCAA CCCTACCTAA CCTCCGCCTC CTTCACTCCT 
TCACCCACTT CACCCACATT TGACTCCAGG TCTGGCAGTG TCATAGAAAT TCTGTCCAGT 
CACAAAGCAG ATTTGCCTGT GAAGGAACTG CAGTTGTTCT CAGATCCCAA AGACAGATAC 
AAGCCAGGCG AGACAGACTC CCCTAAATTG GGCAGCCCTA TTGACTTAGT TGTTTTTGAA 
GGTCAAAAAT CTCCATTGGA TATTCTGCCA CCAAAAGACC TAGAGGATGA TCTGTGTGAG 
GAGCTAGTGT CGATTATAAA GGCCAGCCAA TCAAAGGGTG CATTCTTAGA AGAGGAGGAT 
TTGTATCACC AAGAACCAGA TACTGTGGAA AAGCTACCCA GACTCTTCAT TGAGGAGGAG 
CAGAATGAAG ACAACAGCTT CTTAAATAAA ACCCTGCCAT CAAATACATT CACTCCACTC 
CAACTTGCCC ATAGTGAGGG TTCAGATATT GAGCAGGGTG AAGTTTCCCA GAATGCCTCT 
CCTCGTCTCT CCCCTCTGTC CTCACAGATG TCATTGCCAT TCACATCTGC TAAATACTCC 
CCCACTTCCC CATGCTCCAC CCCACCATTG CCTGGGGTTT CACAGTCACC CCCTCACTTG 
GCAAAACTGT TCCCTCGGCA GGACCTCCGC TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT 
GATGTTGTGG TTGTTGGTAA ACCCCCTCGT AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA 
TCACCTGTTA GAGGTCAAAA CTTTTCGCCA TCTAATAGGT CCCAAAGACA AGCATATGCT 
TGTGATCCAC TTCAAGGTGG AGGGGAACCA TTTCAAGCAC TGTATAACTA CATGCCACGG 
AATGAAGATG AGCTGGAGCT GAAGGAAGGT GATGTTGTTG ATGTCATGGA GAAGTGTGAT 
GATGGATGGT TTGTGGGAAC GTCAAGAAGA ACCAAGTTTT TTGGAACCTT TCCGGGGAAC 
TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481174.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336849.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

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CloneID	ODa148823
Clone ID Related Accession (Same CDS sequence)	XM_021481174.1
Accession Version	XM_021481174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG CTTAAACAAG GTGTACTCCC CAGGGCCAGG 
CAAGCGGCCT TTTCCCCACA TTTTCTTCCG AGCAAGGGGA GTACAGTCCT CGGTTTCAGT 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336849.1
CDS	528..5873
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRLKQGVLPRARQAAFSPHFLPSKGSTVLGFST

Target ORF information:

RefSeq Version	XM_021481174.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481174.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG CTTAAACAAG GTGTACTCCC CAGGGCCAGG 
CAAGCGGCCT TTTCCCCACA TTTTCTTCCG AGCAAGGGGA GTACAGTCCT CGGTTTCAGT 
ACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481176.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336851.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.

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CloneID	ODa148824
Clone ID Related Accession (Same CDS sequence)	XM_021481176.1
Accession Version	XM_021481176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATACCCTCA GTCTGATGCC CACACAGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGCGGT TCACACCAAT GCCCTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTCAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGGGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCG TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336851.1
CDS	344..5665
Translation	MNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481176.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481176.1

ORF Insert Sequence:

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGCACAAGA GCAAAATGTC 
CATACCCTCA GTCTGATGCC CACACAGGAC CAAATTTCAG GCTTTTCACA GCAGTCACAC 
TTGCAACCTC CGTCAAGGGA GCTGGAGAGA CACGTAGCTT CCCTCTCCAT CCACATTCAC 
CCGAGGCCTC AGATATCGGG ACCCCGCACA CAGGCAATGT CTTTGAGACC TCCCACCCCT 
CCTAAAAGGA TGGACCGGCA GGATCGCGGT TCACACCAAT GCCCTCCACT GCCATCTGCA 
GCCCCGCCAG TGCCTGAGGT GCCAGTCCTC CTCAAACAGA GCTATCCTGG AACAGCACAG 
TCCCATAACC GGCTCAGACT CTCCCTCGAT TTCATCAGAC CTGAGACCTT TTCTCCAGCA 
GGCCCTCACT CCCCCGACCG CTGCTCATCC TGTACTCAGA GCGAAGCATC CACTGCAGTG 
CTGGAGGAGT TGAGGGGGTT TGGACTGGGC CCCGAGGGCG GCTCTCGTAC CCCTTCACCA 
ACACTCAGCC AGATGAGTGT TGTCACAGAC AGCATGGGGT TACACTCCCG TGCAGCTAGC 
AACATTAATG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481178.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336853.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.

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CloneID	ODa148825
Clone ID Related Accession (Same CDS sequence)	XM_021481178.1
Accession Version	XM_021481178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGTCCCAA AGACAAGCAT ATGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336853.1
CDS	527..5776
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRVPKTSICL

Target ORF information:

RefSeq Version	XM_021481178.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481178.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGTCCCAA AGACAAGCAT ATGCTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481179.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336854.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.

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CloneID	ODa148826
Clone ID Related Accession (Same CDS sequence)	XM_021481179.1
Accession Version	XM_021481179.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGC CTGAGGTGCC AGTCCTCCTC AAACAGAGCT ATCCTGGAAC AGCACAGTCC 
CATAACCGGC TCAGACTCTC CCTCGATTTC ATCAGACCTG AGACCTTTTC TCCAGCAGGC 
CCTCACTCCC CCGACCGCTG CTCATCCTGT ACTCAGAGCG AAGCATCCAC TGCAGTGCTG 
GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC GAGGGCGGCT CTCGTACCCC TTCACCAACA 
CTCAGCCAGA TGAGTGTTGT CACAGACAGC ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC 
ATTAATGGCC AGATGACTGT AAACGGTGGT CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC 
ATCCAGAGAC CCTTCTCTCC GTCAACATAC CCTCCTCCTC CTTCACTGAG TCCCAGTATT 
ACAGCAATGC AGCAGCAGGC TAGAACCACT GCCTCCAGCA CTCCAATCTA CGCCAACGTA 
GATCCTCCTG CCCGAGCACC GCATACTGAC AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC 
TATACTGGCA TCGGTCCTGT GGATGAGTCT GGGATTCCCA TAGCAATACG GACGTCTGTT 
GACAGGCCAA AAGATTGGTA CAAGACCATG TTCAAACAGA TTCATGTGGT GCCTAAAACA 
GAGATCGAGT GGTCCGGGTC CCGAACTGCC ACAGATCCTG TTACAAGTCC AGCCACAACT 
ACTTCCAAAC AAGATAAGCA AGCTGCCCCT AATGCTGTCC AGGCTCATCC TGCACCAAAA 
ACCAGCACCT ACCGGCCCAT AAGCAAGAGT GTTTCCGACA ATGGCATATA TGCTTTCAGG 
CTGCCCGTCG CCTCTCCCCT GCCCACATCA ACCTCCACAC AGCAGAGACC CATTGAAAGA 
GAGGCTCCAC AGAGAGGCAG AAGCACACCA GACATGAATG AATGGGGGCC TCCTGATAGA 
AAAGTGGACA CACGGAAATA CAGAGCAGAG CCAAGGAGCA TCTTTGACTA TGAGCCTGGA 
AAATCATCTG TTTTAGAACA GGAGAGAGCG AAAAGTAACT TAAGTCCAGA GGACATAGAT 
TTAGAGAATG AGCCGTGGTA TAAGTTCTTT GCTGAACTGG AGTTTGGGCG GCCGCCTCCA 
AAAAAACTTC TGGATTACAA TCCAGAGAGC GCTCCTCGAT TTCCAGTGGA GACCTCTCTT 
TATCAGCCAT CCACTGACAG GAGCTTTGAA AGGCCATCAA GCTCTGCAAG TGATAACAAG 
AAGAGGCGAA AATCCGAACC TGCTACCACT CAGCCAAGAG CTCACAGCAG TGTGGGCACA 
ACACAAACAT CTGTCAGACC ACCAGAGCTG CCCAGGGGCA GCAGCACCCA GAGGAATCCC 
CTCACCAGCC CAAGCTCCCC CGCCACCACT AGAACTAAAG GTGGGGATGT GAGCAATATG 
CACACAGCAC ATTCAAACTG TCTAAGTCCA AACCATTCTA CCTCAGAACC TGTGTATCAA 
AACTATGGTG AAAATGAAGA CAGTTTTATG GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC 
AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA GAGAATTCAG AAGACTGGAT GATCGCAGAG 
GAGACCTCAC CTAAGCTCAA ATCTTGGAGT TGTGATGACC TCCTGTCTGA GGGAAGGGGA 
AGAGAAGTGC AGACCCGTTG TGAAAGCACT GGCTCCTTGT TGCACAATGG GGAACTAGAG 
ACATCACAAT ACTGTGATGT CCAAGAGTCT GAGGATCTTA GGGAGAGGAC CCGCCACCGC 
TCTGCCCACG ATGCACCAGG CTTTCTTAAA CTCTACAAGA AGATGCATCA CATTAACAGG 
CAAGAGTTGA TAAACTCTAC TGTCATCTGC TCTGTCAAAT CCAGGATCCG TAAGTATGAG 
AGCGAACAGC GAAAGGATAG GCGAACCGGC AACAAGGGCT GCAACGAAGA GGTGCCTAGA 
GACATGGTGC ACAACAGGAT TTCTGAATTT GAGAGTCTGA TTCAGAAGTC TAAATCCATG 
CCAAACCTTG GGGAGGAGTG CCTAATCAGA GGTTCTTCCA GGAGGATCAA CAGCCCTAAG 
CGTAGCCTCT CTATTGAGTC GTTGCTAGAT GAAGAACCAC CAGCCAGGAA TCCCCCAGAA 
GGACGGCCCC AATACCCCAA GATAAACAAC CATGTGCCAA TTCACATCCA TGTCACCAGT 
GACCAATTAC AAAACTCTGC CATGCAGAAT GATTATTCGG ACAGTGAGCA TGATGCTGTA 
GTGTCCGATC TTAGTGACTT CATCCAGATT GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG 
TTTGACTTGT GTTCATTTGC CTCCTCCGAA AGTTTCTGTG GATCTGGACA CCACCATCAT 
TTTCACAGGC AACTTGTGAG CTCCTGCAAG GGCCGCTGCC CGGCTTCCTA TACACGCTTC 
ACCACCATGA TCAAGCATGA GAGGGCCAAG CAGGATCGGA GGCAACATTT GCGAGTAGAA 
GATTCTGAGT TTGGTCTCAG TAAGCTTGCA TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG 
AAGAACCTGT CACCTTTGAG TTTTTCCCGA ATGTCAAACT CCAAGAGCTA TGTGTACGAG 
ACTCTAGATT CAGCTCTAAA AGACATCTAT GATCACATCC GCACTGAGAA ACGCAGGAGT 
AGCCTGTCAG AAAACAGCAT CCTTCATAGG TTGTTGGTGG AGTTGCTTCC GGACATTCCT 
GAGAGGAATT CCTCCCTGCA TGCACTGGGG AGAAACAGTC CTACAATTCA ACCTCGCTCT 
TACCACCCAC AACCTGATGG CATGTCCAGT CAGGACATTC ACCAATCCGA GTACTGTCAC 
CTGTCCCATA GTGCCTCTCA CAACCATATA GACAGCAACA ACAACCAGAG GAACCACAAC 
TTTAAATACT GCTATCAAGA CCAGGACACA TCCAGAAATT ACACTTACCC TGATGTGGGC 
CGGCAAACAC CACAGAGCAG GAGACAAACC CCTGATGTCA GAGAGAAACT GCCAGCCAGA 
GCAATATATG ACTTCAAAGC ACAGACGGCC AAAGAGCTGA CGTTCAAAAA AGGAGACACA 
GTATACATCA GTCGGCAGAT AGATAATAAC TGGTATGAAG GAGAATACCA TGGACATGTA 
GGGATATTCC CCATCTCATA TGTGGAGAAA ATCCCTCCAT CAGAGCGACA TCTGCCAGCA 
AGACCACCTC CTCCAGCCCA GAGCAAAGAA ATTGGAGAAG CAGTTGCCCG TTACAACTTT 
AATGCTGATA CTAATGTGGA ACTTTCATTA AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG 
CAGGTGGACA AAAACTGGTT CGAGGGAAAG ATCCCAGGTA CAAACAAACA AGGAATATTT 
CCAGTGTCGT ATGTGGATGT CATTAAGAAG ACTTCAGTAC AAAATACTGG ACAACCGCCT 
GGGCCTAGTA TACCCACCAG CTTCTCCAGT GACAGACTGA ACAGTAGGCC GTCATCTACG 
CGTAACCTCC ACAACTCTCC ATCCCCAACT ATCCGTCCAC TCTCCTCATC TTCTCCCAGT 
CCACAGAGAG CTGCACACCT TCAAGCCATC ACCAGCGAGT GGTTGGCTCT CACTCTGGGT 
CTGTCCCCTT CAGGAACCCC TGCCCCCACA CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA 
TACTATGAAG CGTTTGACTC TGGCATCTCG CCATCTCCCG CCTCCTCCAC ACTGGGCCGC 
TCTCCAACAC TCACTCCTCG CGTCTCCACA CCTGCACTCA AAGAGGGCCA CTTCATTCCC 
ATCTCCTCCC CAAAGTCCTA CATGTCCCCT GATCCCAGCC CGTCACCTCA ACCCTACCTA 
ACCTCCGCCT CCTTCACTCC TTCACCCACT TCACCCACAT TTGACTCCAG GTCTGGCAGT 
GTCATAGAAA TTCTGTCCAG TCACAAAGCA GATTTGCCTG TGAAGGAACT GCAGTTGTTC 
TCAGATCCCA AAGACAGATA CAAGCCAGGC GAGACAGACT CCCCTAAATT GGGCAGCCCT 
ATTGACTTAG TTGTTTTTGA AGGTCAAAAA TCTCCATTGG ATATTCTGCC ACCAAAAGAC 
CTAGAGGATG ATCTGTGTGA GGAGCTAGTG TCGATTATAA AGGCCAGCCA ATCAAAGGGT 
GCATTCTTAG AAGAGGAGGA TTTGTATCAC CAAGAACCAG ATACTGTGGA AAAGCTACCC 
AGACTCTTCA TTGAGGAGGA GCAGAATGAA GACAACAGCT TCTTAAATAA AACCCTGCCA 
TCAAATACAT TCACTCCACT CCAACTTGCC CATAGTGAGG GTTCAGATAT TGAGCAGGGT 
GAAGTTTCCC AGAATGCCTC TCCTCGTCTC TCCCCTCTGT CCTCACAGAT GTCATTGCCA 
TTCACATCTG CTAAATACTC CCCCACTTCC CCATGCTCCA CCCCACCATT GCCTGGGGTT 
TCACAGTCAC CCCCTCACTT GGCAAAACTG TTCCCTCGGC AGGACCTCCG CTCCCCAAAG 
GTCAAGCCTG TGTTAAGGCG TGATGTTGTG GTTGTTGGTA AACCCCCTCG TAGCCCTGTG 
ATGTCTAGGA GGTCCTGCGG ATCACCTGTT AGAGGTCAAA ACTTTTCGCC ATCTAATAGG 
TCCCAAAGAC AAGCATATGC TTGTGATCCA CTTCAAGGTG GAGGGGAACC ATTTCAAGCA 
CTGTATAACT ACATGCCACG GAATGAAGAT GAGCTGGAGC TGAAGGAAGG TGATGTTGTT 
GATGTCATGG AGAAGTGTGA TGATGGATGG TTTGTGGGAA CGTCAAGAAG AACCAAGTTT 
TTTGGAACCT TTCCGGGGAA CTATGTGAAG AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336854.1
CDS	526..5724
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481179.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481179.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGC CTGAGGTGCC AGTCCTCCTC AAACAGAGCT ATCCTGGAAC AGCACAGTCC 
CATAACCGGC TCAGACTCTC CCTCGATTTC ATCAGACCTG AGACCTTTTC TCCAGCAGGC 
CCTCACTCCC CCGACCGCTG CTCATCCTGT ACTCAGAGCG AAGCATCCAC TGCAGTGCTG 
GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC GAGGGCGGCT CTCGTACCCC TTCACCAACA 
CTCAGCCAGA TGAGTGTTGT CACAGACAGC ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC 
ATTAATGGCC AGATGACTGT AAACGGTGGT CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC 
ATCCAGAGAC CCTTCTCTCC GTCAACATAC CCTCCTCCTC CTTCACTGAG TCCCAGTATT 
ACAGCAATGC AGCAGCAGGC TAGAACCACT GCCTCCAGCA CTCCAATCTA CGCCAACGTA 
GATCCTCCTG CCCGAGCACC GCATACTGAC AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC 
TATACTGGCA TCGGTCCTGT GGATGAGTCT GGGATTCCCA TAGCAATACG GACGTCTGTT 
GACAGGCCAA AAGATTGGTA CAAGACCATG TTCAAACAGA TTCATGTGGT GCCTAAAACA 
GAGATCGAGT GGTCCGGGTC CCGAACTGCC ACAGATCCTG TTACAAGTCC AGCCACAACT 
ACTTCCAAAC AAGATAAGCA AGCTGCCCCT AATGCTGTCC AGGCTCATCC TGCACCAAAA 
ACCAGCACCT ACCGGCCCAT AAGCAAGAGT GTTTCCGACA ATGGCATATA TGCTTTCAGG 
CTGCCCGTCG CCTCTCCCCT GCCCACATCA ACCTCCACAC AGCAGAGACC CATTGAAAGA 
GAGGCTCCAC AGAGAGGCAG AAGCACACCA GACATGAATG AATGGGGGCC TCCTGATAGA 
AAAGTGGACA CACGGAAATA CAGAGCAGAG CCAAGGAGCA TCTTTGACTA TGAGCCTGGA 
AAATCATCTG TTTTAGAACA GGAGAGAGCG AAAAGTAACT TAAGTCCAGA GGACATAGAT 
TTAGAGAATG AGCCGTGGTA TAAGTTCTTT GCTGAACTGG AGTTTGGGCG GCCGCCTCCA 
AAAAAACTTC TGGATTACAA TCCAGAGAGC GCTCCTCGAT TTCCAGTGGA GACCTCTCTT 
TATCAGCCAT CCACTGACAG GAGCTTTGAA AGGCCATCAA GCTCTGCAAG TGATAACAAG 
AAGAGGCGAA AATCCGAACC TGCTACCACT CAGCCAAGAG CTCACAGCAG TGTGGGCACA 
ACACAAACAT CTGTCAGACC ACCAGAGCTG CCCAGGGGCA GCAGCACCCA GAGGAATCCC 
CTCACCAGCC CAAGCTCCCC CGCCACCACT AGAACTAAAG GTGGGGATGT GAGCAATATG 
CACACAGCAC ATTCAAACTG TCTAAGTCCA AACCATTCTA CCTCAGAACC TGTGTATCAA 
AACTATGGTG AAAATGAAGA CAGTTTTATG GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC 
AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA GAGAATTCAG AAGACTGGAT GATCGCAGAG 
GAGACCTCAC CTAAGCTCAA ATCTTGGAGT TGTGATGACC TCCTGTCTGA GGGAAGGGGA 
AGAGAAGTGC AGACCCGTTG TGAAAGCACT GGCTCCTTGT TGCACAATGG GGAACTAGAG 
ACATCACAAT ACTGTGATGT CCAAGAGTCT GAGGATCTTA GGGAGAGGAC CCGCCACCGC 
TCTGCCCACG ATGCACCAGG CTTTCTTAAA CTCTACAAGA AGATGCATCA CATTAACAGG 
CAAGAGTTGA TAAACTCTAC TGTCATCTGC TCTGTCAAAT CCAGGATCCG TAAGTATGAG 
AGCGAACAGC GAAAGGATAG GCGAACCGGC AACAAGGGCT GCAACGAAGA GGTGCCTAGA 
GACATGGTGC ACAACAGGAT TTCTGAATTT GAGAGTCTGA TTCAGAAGTC TAAATCCATG 
CCAAACCTTG GGGAGGAGTG CCTAATCAGA GGTTCTTCCA GGAGGATCAA CAGCCCTAAG 
CGTAGCCTCT CTATTGAGTC GTTGCTAGAT GAAGAACCAC CAGCCAGGAA TCCCCCAGAA 
GGACGGCCCC AATACCCCAA GATAAACAAC CATGTGCCAA TTCACATCCA TGTCACCAGT 
GACCAATTAC AAAACTCTGC CATGCAGAAT GATTATTCGG ACAGTGAGCA TGATGCTGTA 
GTGTCCGATC TTAGTGACTT CATCCAGATT GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG 
TTTGACTTGT GTTCATTTGC CTCCTCCGAA AGTTTCTGTG GATCTGGACA CCACCATCAT 
TTTCACAGGC AACTTGTGAG CTCCTGCAAG GGCCGCTGCC CGGCTTCCTA TACACGCTTC 
ACCACCATGA TCAAGCATGA GAGGGCCAAG CAGGATCGGA GGCAACATTT GCGAGTAGAA 
GATTCTGAGT TTGGTCTCAG TAAGCTTGCA TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG 
AAGAACCTGT CACCTTTGAG TTTTTCCCGA ATGTCAAACT CCAAGAGCTA TGTGTACGAG 
ACTCTAGATT CAGCTCTAAA AGACATCTAT GATCACATCC GCACTGAGAA ACGCAGGAGT 
AGCCTGTCAG AAAACAGCAT CCTTCATAGG TTGTTGGTGG AGTTGCTTCC GGACATTCCT 
GAGAGGAATT CCTCCCTGCA TGCACTGGGG AGAAACAGTC CTACAATTCA ACCTCGCTCT 
TACCACCCAC AACCTGATGG CATGTCCAGT CAGGACATTC ACCAATCCGA GTACTGTCAC 
CTGTCCCATA GTGCCTCTCA CAACCATATA GACAGCAACA ACAACCAGAG GAACCACAAC 
TTTAAATACT GCTATCAAGA CCAGGACACA TCCAGAAATT ACACTTACCC TGATGTGGGC 
CGGCAAACAC CACAGAGCAG GAGACAAACC CCTGATGTCA GAGAGAAACT GCCAGCCAGA 
GCAATATATG ACTTCAAAGC ACAGACGGCC AAAGAGCTGA CGTTCAAAAA AGGAGACACA 
GTATACATCA GTCGGCAGAT AGATAATAAC TGGTATGAAG GAGAATACCA TGGACATGTA 
GGGATATTCC CCATCTCATA TGTGGAGAAA ATCCCTCCAT CAGAGCGACA TCTGCCAGCA 
AGACCACCTC CTCCAGCCCA GAGCAAAGAA ATTGGAGAAG CAGTTGCCCG TTACAACTTT 
AATGCTGATA CTAATGTGGA ACTTTCATTA AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG 
CAGGTGGACA AAAACTGGTT CGAGGGAAAG ATCCCAGGTA CAAACAAACA AGGAATATTT 
CCAGTGTCGT ATGTGGATGT CATTAAGAAG ACTTCAGTAC AAAATACTGG ACAACCGCCT 
GGGCCTAGTA TACCCACCAG CTTCTCCAGT GACAGACTGA ACAGTAGGCC GTCATCTACG 
CGTAACCTCC ACAACTCTCC ATCCCCAACT ATCCGTCCAC TCTCCTCATC TTCTCCCAGT 
CCACAGAGAG CTGCACACCT TCAAGCCATC ACCAGCGAGT GGTTGGCTCT CACTCTGGGT 
CTGTCCCCTT CAGGAACCCC TGCCCCCACA CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA 
TACTATGAAG CGTTTGACTC TGGCATCTCG CCATCTCCCG CCTCCTCCAC ACTGGGCCGC 
TCTCCAACAC TCACTCCTCG CGTCTCCACA CCTGCACTCA AAGAGGGCCA CTTCATTCCC 
ATCTCCTCCC CAAAGTCCTA CATGTCCCCT GATCCCAGCC CGTCACCTCA ACCCTACCTA 
ACCTCCGCCT CCTTCACTCC TTCACCCACT TCACCCACAT TTGACTCCAG GTCTGGCAGT 
GTCATAGAAA TTCTGTCCAG TCACAAAGCA GATTTGCCTG TGAAGGAACT GCAGTTGTTC 
TCAGATCCCA AAGACAGATA CAAGCCAGGC GAGACAGACT CCCCTAAATT GGGCAGCCCT 
ATTGACTTAG TTGTTTTTGA AGGTCAAAAA TCTCCATTGG ATATTCTGCC ACCAAAAGAC 
CTAGAGGATG ATCTGTGTGA GGAGCTAGTG TCGATTATAA AGGCCAGCCA ATCAAAGGGT 
GCATTCTTAG AAGAGGAGGA TTTGTATCAC CAAGAACCAG ATACTGTGGA AAAGCTACCC 
AGACTCTTCA TTGAGGAGGA GCAGAATGAA GACAACAGCT TCTTAAATAA AACCCTGCCA 
TCAAATACAT TCACTCCACT CCAACTTGCC CATAGTGAGG GTTCAGATAT TGAGCAGGGT 
GAAGTTTCCC AGAATGCCTC TCCTCGTCTC TCCCCTCTGT CCTCACAGAT GTCATTGCCA 
TTCACATCTG CTAAATACTC CCCCACTTCC CCATGCTCCA CCCCACCATT GCCTGGGGTT 
TCACAGTCAC CCCCTCACTT GGCAAAACTG TTCCCTCGGC AGGACCTCCG CTCCCCAAAG 
GTCAAGCCTG TGTTAAGGCG TGATGTTGTG GTTGTTGGTA AACCCCCTCG TAGCCCTGTG 
ATGTCTAGGA GGTCCTGCGG ATCACCTGTT AGAGGTCAAA ACTTTTCGCC ATCTAATAGG 
TCCCAAAGAC AAGCATATGC TTGTGATCCA CTTCAAGGTG GAGGGGAACC ATTTCAAGCA 
CTGTATAACT ACATGCCACG GAATGAAGAT GAGCTGGAGC TGAAGGAAGG TGATGTTGTT 
GATGTCATGG AGAAGTGTGA TGATGGATGG TTTGTGGGAA CGTCAAGAAG AACCAAGTTT 
TTTGGAACCT TTCCGGGGAA CTATGTGAAG AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481180.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336855.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.

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CloneID	ODa148827
Clone ID Related Accession (Same CDS sequence)	XM_021481180.1
Accession Version	XM_021481180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGGTAA AGACAAGCCT GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336855.1
CDS	527..5683
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKVKTSLC

Target ORF information:

RefSeq Version	XM_021481180.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481180.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGGTAA AGACAAGCCT GTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336856.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X13, mRNA.

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CloneID	ODa148828
Clone ID Related Accession (Same CDS sequence)	XM_021481181.1
Accession Version	XM_021481181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGCCTGAGG TGCCAGTCCT CCTCAAACAG 
AGCTATCCTG GAACAGCACA GTCCCATAAC CGGCTCAGAC TCTCCCTCGA TTTCATCAGA 
CCTGAGACCT TTTCTCCAGC AGGCCCTCAC TCCCCCGACC GCTGCTCATC CTGTACTCAG 
AGCGAAGCAT CCACTGCAGT GCTGGAGGAG TTGAGGGGGT TTGGACTGGG CCCCGAGGGC 
GGCTCTCGTA CCCCTTCACC AACACTCAGC CAGATGAGTG TTGTCACAGA CAGCATGGGG 
TTACACTCCC GTGCAGCTAG CAACATTAAT GGCCAGATGA CTGTAAACGG TGGTCTGAGT 
GTGCCAGCAA GTCCTCGTAG TCACATCCAG AGACCCTTCT CTCCGTCAAC ATACCCTCCT 
CCTCCTTCAC TGAGTCCCAG TATTACAGCA ATGCAGCAGC AGGCTAGAAC CACTGCCTCC 
AGCACTCCAA TCTACGCCAA CGTAGATCCT CCTGCCCGAG CACCGCATAC TGACAGGAGA 
GAAACAGCAG GGAAAGCTCC GCACTATACT GGCATCGGTC CTGTGGATGA GTCTGGGATT 
CCCATAGCAA TACGGACGTC TGTTGACAGG CCAAAAGATT GGTACAAGAC CATGTTCAAA 
CAGATTCATG TGGTGCCTAA AACAGAGATC GAGTGGTCCG GGTCCCGAAC TGCCACAGAT 
CCTGTTACAA GTCCAGCCAC AACTACTTCC AAACAAGATA AGCAAGCTGC CCCTAATGCT 
GTCCAGGCTC ATCCTGCACC AAAAACCAGC ACCTACCGGC CCATAAGCAA GAGTGTTTCC 
GACAATGGCA TATATGCTTT CAGGCTGCCC GTCGCCTCTC CCCTGCCCAC ATCAACCTCC 
ACACAGCAGA GACCCATTGA AAGAGAGGCT CCACAGAGAG GCAGAAGCAC ACCAGACATG 
AATGAATGGG GGCCTCCTGA TAGAAAAGTG GACACACGGA AATACAGAGC AGAGCCAAGG 
AGCATCTTTG ACTATGAGCC TGGAAAATCA TCTGTTTTAG AACAGGAGAG AGCGAAAAGT 
AACTTAAGTC CAGAGGACAT AGATTTAGAG AATGAGCCGT GGTATAAGTT CTTTGCTGAA 
CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA CTTCTGGATT ACAATCCAGA GAGCGCTCCT 
CGATTTCCAG TGGAGACCTC TCTTTATCAG CCATCCACTG ACAGGAGCTT TGAAAGGCCA 
TCAAGCTCTG CAAGTGATAA CAAGAAGAGG CGAAAATCCG AACCTGCTAC CACTCAGCCA 
AGAGCTCACA GCAGTGTGGG CACAACACAA ACATCTGTCA GACCACCAGA GCTGCCCAGG 
GGCAGCAGCA CCCAGAGGAA TCCCCTCACC AGCCCAAGCT CCCCCGCCAC CACTAGAACT 
AAAGGTGGGG ATGTGAGCAA TATGCACACA GCACATTCAA ACTGTCTAAG TCCAAACCAT 
TCTACCTCAG AACCTGTGTA TCAAAACTAT GGTGAAAATG AAGACAGTTT TATGGCTGTC 
CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC CCAGAAGGCT GGAAACCAGC CCAAGAGAAT 
TCAGAAGACT GGATGATCGC AGAGGAGACC TCACCTAAGC TCAAATCTTG GAGTTGTGAT 
GACCTCCTGT CTGAGGGAAG GGGAAGAGAA GTGCAGACCC GTTGTGAAAG CACTGGCTCC 
TTGTTGCACA ATGGGGAACT AGAGACATCA CAATACTGTG ATGTCCAAGA GTCTGAGGAT 
CTTAGGGAGA GGACCCGCCA CCGCTCTGCC CACGATGCAC CAGGCTTTCT TAAACTCTAC 
AAGAAGATGC ATCACATTAA CAGGCAAGAG TTGATAAACT CTACTGTCAT CTGCTCTGTC 
AAATCCAGGA TCCGTAAGTA TGAGAGCGAA CAGCGAAAGG ATAGGCGAAC CGGCAACAAG 
GGCTGCAACG AAGAGGTGCC TAGAGACATG GTGCACAACA GGATTTCTGA ATTTGAGAGT 
CTGATTCAGA AGTCTAAATC CATGCCAAAC CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT 
TCCAGGAGGA TCAACAGCCC TAAGCGTAGC CTCTCTATTG AGTCGTTGCT AGATGAAGAA 
CCACCAGCCA GGAATCCCCC AGAAGGACGG CCCCAATACC CCAAGATAAA CAACCATGTG 
CCAATTCACA TCCATGTCAC CAGTGACCAA TTACAAAACT CTGCCATGCA GAATGATTAT 
TCGGACAGTG AGCATGATGC TGTAGTGTCC GATCTTAGTG ACTTCATCCA GATTGAAGGG 
TCATCTTTTT GCAGTGAGAG GGAGTTTGAC TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC 
TGTGGATCTG GACACCACCA TCATTTTCAC AGGCAACTTG TGAGCTCCTG CAAGGGCCGC 
TGCCCGGCTT CCTATACACG CTTCACCACC ATGATCAAGC ATGAGAGGGC CAAGCAGGAT 
CGGAGGCAAC ATTTGCGAGT AGAAGATTCT GAGTTTGGTC TCAGTAAGCT TGCATTCCTG 
GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC CTGTCACCTT TGAGTTTTTC CCGAATGTCA 
AACTCCAAGA GCTATGTGTA CGAGACTCTA GATTCAGCTC TAAAAGACAT CTATGATCAC 
ATCCGCACTG AGAAACGCAG GAGTAGCCTG TCAGAAAACA GCATCCTTCA TAGGTTGTTG 
GTGGAGTTGC TTCCGGACAT TCCTGAGAGG AATTCCTCCC TGCATGCACT GGGGAGAAAC 
AGTCCTACAA TTCAACCTCG CTCTTACCAC CCACAACCTG ATGGCATGTC CAGTCAGGAC 
ATTCACCAAT CCGAGTACTG TCACCTGTCC CATAGTGCCT CTCACAACCA TATAGACAGC 
AACAACAACC AGAGGAACCA CAACTTTAAA TACTGCTATC AAGACCAGGA CACATCCAGA 
AATTACACTT ACCCTGATGT GGGCCGGCAA ACACCACAGA GCAGGAGACA AACCCCTGAT 
GTCAGAGAGA AACTGCCAGC CAGAGCAATA TATGACTTCA AAGCACAGAC GGCCAAAGAG 
CTGACGTTCA AAAAAGGAGA CACAGTATAC ATCAGTCGGC AGATAGATAA TAACTGGTAT 
GAAGGAGAAT ACCATGGACA TGTAGGGATA TTCCCCATCT CATATGTGGA GAAAATCCCT 
CCATCAGAGC GACATCTGCC AGCAAGACCA CCTCCTCCAG CCCAGAGCAA AGAAATTGGA 
GAAGCAGTTG CCCGTTACAA CTTTAATGCT GATACTAATG TGGAACTTTC ATTAAGAAAA 
GGAGAGCGTG TTGTATTGGT GCGGCAGGTG GACAAAAACT GGTTCGAGGG AAAGATCCCA 
GGTACAAACA AACAAGGAAT ATTTCCAGTG TCGTATGTGG ATGTCATTAA GAAGACTTCA 
GTACAAAATA CTGGACAACC GCCTGGGCCT AGTATACCCA CCAGCTTCTC CAGTGACAGA 
CTGAACAGTA GGCCGTCATC TACGCGTAAC CTCCACAACT CTCCATCCCC AACTATCCGT 
CCACTCTCCT CATCTTCTCC CAGTCCACAG AGAGCTGCAC ACCTTCAAGC CATCACCAGC 
GAGTGGTTGG CTCTCACTCT GGGTCTGTCC CCTTCAGGAA CCCCTGCCCC CACACCTCCT 
CCCTTCCCTT CCAATTTCCA GCCATACTAT GAAGCGTTTG ACTCTGGCAT CTCGCCATCT 
CCCGCCTCCT CCACACTGGG CCGCTCTCCA ACACTCACTC CTCGCGTCTC CACACCTGCA 
CTCAAAGAGG GCCACTTCAT TCCCATCTCC TCCCCAAAGT CCTACATGTC CCCTGATCCC 
AGCCCGTCAC CTCAACCCTA CCTAACCTCC GCCTCCTTCA CTCCTTCACC CACTTCACCC 
ACATTTGACT CCAGGTCTGG CAGTGTCATA GAAATTCTGT CCAGTCACAA AGCAGATTTG 
CCTGTGAAGG AACTGCAGTT GTTCTCAGAT CCCAAAGACA GATACAAGCC AGGCGAGACA 
GACTCCCCTA AATTGGGCAG CCCTATTGAC TTAGTTGTTT TTGAAGGTCA AAAATCTCCA 
TTGGATATTC TGCCACCAAA AGACCTAGAG GATGATCTGT GTGAGGAGCT AGTGTCGATT 
ATAAAGGCCA GCCAATCAAA GGGTGCATTC TTAGAAGAGG AGGATTTGTA TCACCAAGAA 
CCAGATACTG TGGAAAAGCT ACCCAGACTC TTCATTGAGG AGGAGCAGAA TGAAGACAAC 
AGCTTCTTAA ATAAAACCCT GCCATCAAAT ACATTCACTC CACTCCAACT TGCCCATAGT 
GAGGGTTCAG ATATTGAGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCCCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAGAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GAAGAACCAA GTTTTTTGGA ACCTTTCCGG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336856.1
CDS	525..5627
Translation	MPGLCDIKTLQEGEIFNMNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481181.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481181.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGCCTGAGG TGCCAGTCCT CCTCAAACAG 
AGCTATCCTG GAACAGCACA GTCCCATAAC CGGCTCAGAC TCTCCCTCGA TTTCATCAGA 
CCTGAGACCT TTTCTCCAGC AGGCCCTCAC TCCCCCGACC GCTGCTCATC CTGTACTCAG 
AGCGAAGCAT CCACTGCAGT GCTGGAGGAG TTGAGGGGGT TTGGACTGGG CCCCGAGGGC 
GGCTCTCGTA CCCCTTCACC AACACTCAGC CAGATGAGTG TTGTCACAGA CAGCATGGGG 
TTACACTCCC GTGCAGCTAG CAACATTAAT GGCCAGATGA CTGTAAACGG TGGTCTGAGT 
GTGCCAGCAA GTCCTCGTAG TCACATCCAG AGACCCTTCT CTCCGTCAAC ATACCCTCCT 
CCTCCTTCAC TGAGTCCCAG TATTACAGCA ATGCAGCAGC AGGCTAGAAC CACTGCCTCC 
AGCACTCCAA TCTACGCCAA CGTAGATCCT CCTGCCCGAG CACCGCATAC TGACAGGAGA 
GAAACAGCAG GGAAAGCTCC GCACTATACT GGCATCGGTC CTGTGGATGA GTCTGGGATT 
CCCATAGCAA TACGGACGTC TGTTGACAGG CCAAAAGATT GGTACAAGAC CATGTTCAAA 
CAGATTCATG TGGTGCCTAA AACAGAGATC GAGTGGTCCG GGTCCCGAAC TGCCACAGAT 
CCTGTTACAA GTCCAGCCAC AACTACTTCC AAACAAGATA AGCAAGCTGC CCCTAATGCT 
GTCCAGGCTC ATCCTGCACC AAAAACCAGC ACCTACCGGC CCATAAGCAA GAGTGTTTCC 
GACAATGGCA TATATGCTTT CAGGCTGCCC GTCGCCTCTC CCCTGCCCAC ATCAACCTCC 
ACACAGCAGA GACCCATTGA AAGAGAGGCT CCACAGAGAG GCAGAAGCAC ACCAGACATG 
AATGAATGGG GGCCTCCTGA TAGAAAAGTG GACACACGGA AATACAGAGC AGAGCCAAGG 
AGCATCTTTG ACTATGAGCC TGGAAAATCA TCTGTTTTAG AACAGGAGAG AGCGAAAAGT 
AACTTAAGTC CAGAGGACAT AGATTTAGAG AATGAGCCGT GGTATAAGTT CTTTGCTGAA 
CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA CTTCTGGATT ACAATCCAGA GAGCGCTCCT 
CGATTTCCAG TGGAGACCTC TCTTTATCAG CCATCCACTG ACAGGAGCTT TGAAAGGCCA 
TCAAGCTCTG CAAGTGATAA CAAGAAGAGG CGAAAATCCG AACCTGCTAC CACTCAGCCA 
AGAGCTCACA GCAGTGTGGG CACAACACAA ACATCTGTCA GACCACCAGA GCTGCCCAGG 
GGCAGCAGCA CCCAGAGGAA TCCCCTCACC AGCCCAAGCT CCCCCGCCAC CACTAGAACT 
AAAGGTGGGG ATGTGAGCAA TATGCACACA GCACATTCAA ACTGTCTAAG TCCAAACCAT 
TCTACCTCAG AACCTGTGTA TCAAAACTAT GGTGAAAATG AAGACAGTTT TATGGCTGTC 
CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC CCAGAAGGCT GGAAACCAGC CCAAGAGAAT 
TCAGAAGACT GGATGATCGC AGAGGAGACC TCACCTAAGC TCAAATCTTG GAGTTGTGAT 
GACCTCCTGT CTGAGGGAAG GGGAAGAGAA GTGCAGACCC GTTGTGAAAG CACTGGCTCC 
TTGTTGCACA ATGGGGAACT AGAGACATCA CAATACTGTG ATGTCCAAGA GTCTGAGGAT 
CTTAGGGAGA GGACCCGCCA CCGCTCTGCC CACGATGCAC CAGGCTTTCT TAAACTCTAC 
AAGAAGATGC ATCACATTAA CAGGCAAGAG TTGATAAACT CTACTGTCAT CTGCTCTGTC 
AAATCCAGGA TCCGTAAGTA TGAGAGCGAA CAGCGAAAGG ATAGGCGAAC CGGCAACAAG 
GGCTGCAACG AAGAGGTGCC TAGAGACATG GTGCACAACA GGATTTCTGA ATTTGAGAGT 
CTGATTCAGA AGTCTAAATC CATGCCAAAC CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT 
TCCAGGAGGA TCAACAGCCC TAAGCGTAGC CTCTCTATTG AGTCGTTGCT AGATGAAGAA 
CCACCAGCCA GGAATCCCCC AGAAGGACGG CCCCAATACC CCAAGATAAA CAACCATGTG 
CCAATTCACA TCCATGTCAC CAGTGACCAA TTACAAAACT CTGCCATGCA GAATGATTAT 
TCGGACAGTG AGCATGATGC TGTAGTGTCC GATCTTAGTG ACTTCATCCA GATTGAAGGG 
TCATCTTTTT GCAGTGAGAG GGAGTTTGAC TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC 
TGTGGATCTG GACACCACCA TCATTTTCAC AGGCAACTTG TGAGCTCCTG CAAGGGCCGC 
TGCCCGGCTT CCTATACACG CTTCACCACC ATGATCAAGC ATGAGAGGGC CAAGCAGGAT 
CGGAGGCAAC ATTTGCGAGT AGAAGATTCT GAGTTTGGTC TCAGTAAGCT TGCATTCCTG 
GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC CTGTCACCTT TGAGTTTTTC CCGAATGTCA 
AACTCCAAGA GCTATGTGTA CGAGACTCTA GATTCAGCTC TAAAAGACAT CTATGATCAC 
ATCCGCACTG AGAAACGCAG GAGTAGCCTG TCAGAAAACA GCATCCTTCA TAGGTTGTTG 
GTGGAGTTGC TTCCGGACAT TCCTGAGAGG AATTCCTCCC TGCATGCACT GGGGAGAAAC 
AGTCCTACAA TTCAACCTCG CTCTTACCAC CCACAACCTG ATGGCATGTC CAGTCAGGAC 
ATTCACCAAT CCGAGTACTG TCACCTGTCC CATAGTGCCT CTCACAACCA TATAGACAGC 
AACAACAACC AGAGGAACCA CAACTTTAAA TACTGCTATC AAGACCAGGA CACATCCAGA 
AATTACACTT ACCCTGATGT GGGCCGGCAA ACACCACAGA GCAGGAGACA AACCCCTGAT 
GTCAGAGAGA AACTGCCAGC CAGAGCAATA TATGACTTCA AAGCACAGAC GGCCAAAGAG 
CTGACGTTCA AAAAAGGAGA CACAGTATAC ATCAGTCGGC AGATAGATAA TAACTGGTAT 
GAAGGAGAAT ACCATGGACA TGTAGGGATA TTCCCCATCT CATATGTGGA GAAAATCCCT 
CCATCAGAGC GACATCTGCC AGCAAGACCA CCTCCTCCAG CCCAGAGCAA AGAAATTGGA 
GAAGCAGTTG CCCGTTACAA CTTTAATGCT GATACTAATG TGGAACTTTC ATTAAGAAAA 
GGAGAGCGTG TTGTATTGGT GCGGCAGGTG GACAAAAACT GGTTCGAGGG AAAGATCCCA 
GGTACAAACA AACAAGGAAT ATTTCCAGTG TCGTATGTGG ATGTCATTAA GAAGACTTCA 
GTACAAAATA CTGGACAACC GCCTGGGCCT AGTATACCCA CCAGCTTCTC CAGTGACAGA 
CTGAACAGTA GGCCGTCATC TACGCGTAAC CTCCACAACT CTCCATCCCC AACTATCCGT 
CCACTCTCCT CATCTTCTCC CAGTCCACAG AGAGCTGCAC ACCTTCAAGC CATCACCAGC 
GAGTGGTTGG CTCTCACTCT GGGTCTGTCC CCTTCAGGAA CCCCTGCCCC CACACCTCCT 
CCCTTCCCTT CCAATTTCCA GCCATACTAT GAAGCGTTTG ACTCTGGCAT CTCGCCATCT 
CCCGCCTCCT CCACACTGGG CCGCTCTCCA ACACTCACTC CTCGCGTCTC CACACCTGCA 
CTCAAAGAGG GCCACTTCAT TCCCATCTCC TCCCCAAAGT CCTACATGTC CCCTGATCCC 
AGCCCGTCAC CTCAACCCTA CCTAACCTCC GCCTCCTTCA CTCCTTCACC CACTTCACCC 
ACATTTGACT CCAGGTCTGG CAGTGTCATA GAAATTCTGT CCAGTCACAA AGCAGATTTG 
CCTGTGAAGG AACTGCAGTT GTTCTCAGAT CCCAAAGACA GATACAAGCC AGGCGAGACA 
GACTCCCCTA AATTGGGCAG CCCTATTGAC TTAGTTGTTT TTGAAGGTCA AAAATCTCCA 
TTGGATATTC TGCCACCAAA AGACCTAGAG GATGATCTGT GTGAGGAGCT AGTGTCGATT 
ATAAAGGCCA GCCAATCAAA GGGTGCATTC TTAGAAGAGG AGGATTTGTA TCACCAAGAA 
CCAGATACTG TGGAAAAGCT ACCCAGACTC TTCATTGAGG AGGAGCAGAA TGAAGACAAC 
AGCTTCTTAA ATAAAACCCT GCCATCAAAT ACATTCACTC CACTCCAACT TGCCCATAGT 
GAGGGTTCAG ATATTGAGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCCCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAGAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GAAGAACCAA GTTTTTTGGA ACCTTTCCGG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481183.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336858.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa148829
Clone ID Related Accession (Same CDS sequence)	XM_021481183.1
Accession Version	XM_021481183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGGCCAGAT GACTGTAAAC 
GGTGGTCTGA GTGTGCCAGC AAGTCCTCGT AGTCACATCC AGAGACCCTT CTCTCCGTCA 
ACATACCCTC CTCCTCCTTC ACTGAGTCCC AGTATTACAG CAATGCAGCA GCAGGCTAGA 
ACCACTGCCT CCAGCACTCC AATCTACGCC AACGTAGATC CTCCTGCCCG AGCACCGCAT 
ACTGACAGGA GAGAAACAGC AGGGAAAGCT CCGCACTATA CTGGCATCGG TCCTGTGGAT 
GAGTCTGGGA TTCCCATAGC AATACGGACG TCTGTTGACA GGCCAAAAGA TTGGTACAAG 
ACCATGTTCA AACAGATTCA TGTGGTGCCT AAAACAGAGA TCGAGTGGTC CGGGTCCCGA 
ACTGCCACAG ATCCTGTTAC AAGTCCAGCC ACAACTACTT CCAAACAAGA TAAGCAAGCT 
GCCCCTAATG CTGTCCAGGC TCATCCTGCA CCAAAAACCA GCACCTACCG GCCCATAAGC 
AAGAGTGTTT CCGACAATGG CATATATGCT TTCAGGCTGC CCGTCGCCTC TCCCCTGCCC 
ACATCAACCT CCACACAGCA GAGACCCATT GAAAGAGAGG CTCCACAGAG AGGCAGAAGC 
ACACCAGACA TGAATGAATG GGGGCCTCCT GATAGAAAAG TGGACACACG GAAATACAGA 
GCAGAGCCAA GGAGCATCTT TGACTATGAG CCTGGAAAAT CATCTGTTTT AGAACAGGAG 
AGAGCGAAAA GTAACTTAAG TCCAGAGGAC ATAGATTTAG AGAATGAGCC GTGGTATAAG 
TTCTTTGCTG AACTGGAGTT TGGGCGGCCG CCTCCAAAAA AACTTCTGGA TTACAATCCA 
GAGAGCGCTC CTCGATTTCC AGTGGAGACC TCTCTTTATC AGCCATCCAC TGACAGGAGC 
TTTGAAAGGC CATCAAGCTC TGCAAGTGAT AACAAGAAGA GGCGAAAATC CGAACCTGCT 
ACCACTCAGC CAAGAGCTCA CAGCAGTGTG GGCACAACAC AAACATCTGT CAGACCACCA 
GAGCTGCCCA GGGGCAGCAG CACCCAGAGG AATCCCCTCA CCAGCCCAAG CTCCCCCGCC 
ACCACTAGAA CTAAAGGTGG GGATGTGAGC AATATGCACA CAGCACATTC AAACTGTCTA 
AGTCCAAACC ATTCTACCTC AGAACCTGTG TATCAAAACT ATGGTGAAAA TGAAGACAGT 
TTTATGGCTG TCCCAGCTGT CTCAGAAGGC TTCTCCAGCC TCCCAGAAGG CTGGAAACCA 
GCCCAAGAGA ATTCAGAAGA CTGGATGATC GCAGAGGAGA CCTCACCTAA GCTCAAATCT 
TGGAGTTGTG ATGACCTCCT GTCTGAGGGA AGGGGAAGAG AAGTGCAGAC CCGTTGTGAA 
AGCACTGGCT CCTTGTTGCA CAATGGGGAA CTAGAGACAT CACAATACTG TGATGTCCAA 
GAGTCTGAGG ATCTTAGGGA GAGGACCCGC CACCGCTCTG CCCACGATGC ACCAGGCTTT 
CTTAAACTCT ACAAGAAGAT GCATCACATT AACAGGCAAG AGTTGATAAA CTCTACTGTC 
ATCTGCTCTG TCAAATCCAG GATCCGTAAG TATGAGAGCG AACAGCGAAA GGATAGGCGA 
ACCGGCAACA AGGGCTGCAA CGAAGAGGTG CCTAGAGACA TGGTGCACAA CAGGATTTCT 
GAATTTGAGA GTCTGATTCA GAAGTCTAAA TCCATGCCAA ACCTTGGGGA GGAGTGCCTA 
ATCAGAGGTT CTTCCAGGAG GATCAACAGC CCTAAGCGTA GCCTCTCTAT TGAGTCGTTG 
CTAGATGAAG AACCACCAGC CAGGAATCCC CCAGAAGGAC GGCCCCAATA CCCCAAGATA 
AACAACCATG TGCCAATTCA CATCCATGTC ACCAGTGACC AATTACAAAA CTCTGCCATG 
CAGAATGATT ATTCGGACAG TGAGCATGAT GCTGTAGTGT CCGATCTTAG TGACTTCATC 
CAGATTGAAG GGTCATCTTT TTGCAGTGAG AGGGAGTTTG ACTTGTGTTC ATTTGCCTCC 
TCCGAAAGTT TCTGTGGATC TGGACACCAC CATCATTTTC ACAGGCAACT TGTGAGCTCC 
TGCAAGGGCC GCTGCCCGGC TTCCTATACA CGCTTCACCA CCATGATCAA GCATGAGAGG 
GCCAAGCAGG ATCGGAGGCA ACATTTGCGA GTAGAAGATT CTGAGTTTGG TCTCAGTAAG 
CTTGCATTCC TGGTCAGCCC AGTGCCTTTC CGTCGGAAGA ACCTGTCACC TTTGAGTTTT 
TCCCGAATGT CAAACTCCAA GAGCTATGTG TACGAGACTC TAGATTCAGC TCTAAAAGAC 
ATCTATGATC ACATCCGCAC TGAGAAACGC AGGAGTAGCC TGTCAGAAAA CAGCATCCTT 
CATAGGTTGT TGGTGGAGTT GCTTCCGGAC ATTCCTGAGA GGAATTCCTC CCTGCATGCA 
CTGGGGAGAA ACAGTCCTAC AATTCAACCT CGCTCTTACC ACCCACAACC TGATGGCATG 
TCCAGTCAGG ACATTCACCA ATCCGAGTAC TGTCACCTGT CCCATAGTGC CTCTCACAAC 
CATATAGACA GCAACAACAA CCAGAGGAAC CACAACTTTA AATACTGCTA TCAAGACCAG 
GACACATCCA GAAATTACAC TTACCCTGAT GTGGGCCGGC AAACACCACA GAGCAGGAGA 
CAAACCCCTG ATGTCAGAGA GAAACTGCCA GCCAGAGCAA TATATGACTT CAAAGCACAG 
ACGGCCAAAG AGCTGACGTT CAAAAAAGGA GACACAGTAT ACATCAGTCG GCAGATAGAT 
AATAACTGGT ATGAAGGAGA ATACCATGGA CATGTAGGGA TATTCCCCAT CTCATATGTG 
GAGAAAATCC CTCCATCAGA GCGACATCTG CCAGCAAGAC CACCTCCTCC AGCCCAGAGC 
AAAGAAATTG GAGAAGCAGT TGCCCGTTAC AACTTTAATG CTGATACTAA TGTGGAACTT 
TCATTAAGAA AAGGAGAGCG TGTTGTATTG GTGCGGCAGG TGGACAAAAA CTGGTTCGAG 
GGAAAGATCC CAGGTACAAA CAAACAAGGA ATATTTCCAG TGTCGTATGT GGATGTCATT 
AAGAAGACTT CAGTACAAAA TACTGGACAA CCGCCTGGGC CTAGTATACC CACCAGCTTC 
TCCAGTGACA GACTGAACAG TAGGCCGTCA TCTACGCGTA ACCTCCACAA CTCTCCATCC 
CCAACTATCC GTCCACTCTC CTCATCTTCT CCCAGTCCAC AGAGAGCTGC ACACCTTCAA 
GCCATCACCA GCGAGTGGTT GGCTCTCACT CTGGGTCTGT CCCCTTCAGG AACCCCTGCC 
CCCACACCTC CTCCCTTCCC TTCCAATTTC CAGCCATACT ATGAAGCGTT TGACTCTGGC 
ATCTCGCCAT CTCCCGCCTC CTCCACACTG GGCCGCTCTC CAACACTCAC TCCTCGCGTC 
TCCACACCTG CACTCAAAGA GGGCCACTTC ATTCCCATCT CCTCCCCAAA GTCCTACATG 
TCCCCTGATC CCAGCCCGTC ACCTCAACCC TACCTAACCT CCGCCTCCTT CACTCCTTCA 
CCCACTTCAC CCACATTTGA CTCCAGGTCT GGCAGTGTCA TAGAAATTCT GTCCAGTCAC 
AAAGCAGATT TGCCTGTGAA GGAACTGCAG TTGTTCTCAG ATCCCAAAGA CAGATACAAG 
CCAGGCGAGA CAGACTCCCC TAAATTGGGC AGCCCTATTG ACTTAGTTGT TTTTGAAGGT 
CAAAAATCTC CATTGGATAT TCTGCCACCA AAAGACCTAG AGGATGATCT GTGTGAGGAG 
CTAGTGTCGA TTATAAAGGC CAGCCAATCA AAGGGTGCAT TCTTAGAAGA GGAGGATTTG 
TATCACCAAG AACCAGATAC TGTGGAAAAG CTACCCAGAC TCTTCATTGA GGAGGAGCAG 
AATGAAGACA ACAGCTTCTT AAATAAAACC CTGCCATCAA ATACATTCAC TCCACTCCAA 
CTTGCCCATA GTGAGGGTTC AGATATTGAG CAGGGTGAAG TTTCCCAGAA TGCCTCTCCT 
CGTCTCTCCC CTCTGTCCTC ACAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336858.1
CDS	338..5092
Translation	MNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481183.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481183.1

ORF Insert Sequence:

ATGAACACAG ACTCTGAATC ACAATCCAGA GATTCAGATT TGTGGAGGCC CAGTAGTAGC 
ACTGATGGTC TAAGAAACGG TGACGTCTGC TCTTCCTCTC TGGCAGCTAA AGGTTACAGA 
AGTGTCAGGC CCATCTTTCA GGAAAAAAAG TCTCCCACAC CGGGCCAGAT GACTGTAAAC 
GGTGGTCTGA GTGTGCCAGC AAGTCCTCGT AGTCACATCC AGAGACCCTT CTCTCCGTCA 
ACATACCCTC CTCCTCCTTC ACTGAGTCCC AGTATTACAG CAATGCAGCA GCAGGCTAGA 
ACCACTGCCT CCAGCACTCC AATCTACGCC AACGTAGATC CTCCTGCCCG AGCACCGCAT 
ACTGACAGGA GAGAAACAGC AGGGAAAGCT CCGCACTATA CTGGCATCGG TCCTGTGGAT 
GAGTCTGGGA TTCCCATAGC AATACGGACG TCTGTTGACA GGCCAAAAGA TTGGTACAAG 
ACCATGTTCA AACAGATTCA TGTGGTGCCT AAAACAGAGA TCGAGTGGTC CGGGTCCCGA 
ACTGCCACAG ATCCTGTTAC AAGTCCAGCC ACAACTACTT CCAAACAAGA TAAGCAAGCT 
GCCCCTAATG CTGTCCAGGC TCATCCTGCA CCAAAAACCA GCACCTACCG GCCCATAAGC 
AAGAGTGTTT CCGACAATGG CATATATGCT TTCAGGCTGC CCGTCGCCTC TCCCCTGCCC 
ACATCAACCT CCACACAGCA GAGACCCATT GAAAGAGAGG CTCCACAGAG AGGCAGAAGC 
ACACCAGACA TGAATGAATG GGGGCCTCCT GATAGAAAAG TGGACACACG GAAATACAGA 
GCAGAGCCAA GGAGCATCTT TGACTATGAG CCTGGAAAAT CATCTGTTTT AGAACAGGAG 
AGAGCGAAAA GTAACTTAAG TCCAGAGGAC ATAGATTTAG AGAATGAGCC GTGGTATAAG 
TTCTTTGCTG AACTGGAGTT TGGGCGGCCG CCTCCAAAAA AACTTCTGGA TTACAATCCA 
GAGAGCGCTC CTCGATTTCC AGTGGAGACC TCTCTTTATC AGCCATCCAC TGACAGGAGC 
TTTGAAAGGC CATCAAGCTC TGCAAGTGAT AACAAGAAGA GGCGAAAATC CGAACCTGCT 
ACCACTCAGC CAAGAGCTCA CAGCAGTGTG GGCACAACAC AAACATCTGT CAGACCACCA 
GAGCTGCCCA GGGGCAGCAG CACCCAGAGG AATCCCCTCA CCAGCCCAAG CTCCCCCGCC 
ACCACTAGAA CTAAAGGTGG GGATGTGAGC AATATGCACA CAGCACATTC AAACTGTCTA 
AGTCCAAACC ATTCTACCTC AGAACCTGTG TATCAAAACT ATGGTGAAAA TGAAGACAGT 
TTTATGGCTG TCCCAGCTGT CTCAGAAGGC TTCTCCAGCC TCCCAGAAGG CTGGAAACCA 
GCCCAAGAGA ATTCAGAAGA CTGGATGATC GCAGAGGAGA CCTCACCTAA GCTCAAATCT 
TGGAGTTGTG ATGACCTCCT GTCTGAGGGA AGGGGAAGAG AAGTGCAGAC CCGTTGTGAA 
AGCACTGGCT CCTTGTTGCA CAATGGGGAA CTAGAGACAT CACAATACTG TGATGTCCAA 
GAGTCTGAGG ATCTTAGGGA GAGGACCCGC CACCGCTCTG CCCACGATGC ACCAGGCTTT 
CTTAAACTCT ACAAGAAGAT GCATCACATT AACAGGCAAG AGTTGATAAA CTCTACTGTC 
ATCTGCTCTG TCAAATCCAG GATCCGTAAG TATGAGAGCG AACAGCGAAA GGATAGGCGA 
ACCGGCAACA AGGGCTGCAA CGAAGAGGTG CCTAGAGACA TGGTGCACAA CAGGATTTCT 
GAATTTGAGA GTCTGATTCA GAAGTCTAAA TCCATGCCAA ACCTTGGGGA GGAGTGCCTA 
ATCAGAGGTT CTTCCAGGAG GATCAACAGC CCTAAGCGTA GCCTCTCTAT TGAGTCGTTG 
CTAGATGAAG AACCACCAGC CAGGAATCCC CCAGAAGGAC GGCCCCAATA CCCCAAGATA 
AACAACCATG TGCCAATTCA CATCCATGTC ACCAGTGACC AATTACAAAA CTCTGCCATG 
CAGAATGATT ATTCGGACAG TGAGCATGAT GCTGTAGTGT CCGATCTTAG TGACTTCATC 
CAGATTGAAG GGTCATCTTT TTGCAGTGAG AGGGAGTTTG ACTTGTGTTC ATTTGCCTCC 
TCCGAAAGTT TCTGTGGATC TGGACACCAC CATCATTTTC ACAGGCAACT TGTGAGCTCC 
TGCAAGGGCC GCTGCCCGGC TTCCTATACA CGCTTCACCA CCATGATCAA GCATGAGAGG 
GCCAAGCAGG ATCGGAGGCA ACATTTGCGA GTAGAAGATT CTGAGTTTGG TCTCAGTAAG 
CTTGCATTCC TGGTCAGCCC AGTGCCTTTC CGTCGGAAGA ACCTGTCACC TTTGAGTTTT 
TCCCGAATGT CAAACTCCAA GAGCTATGTG TACGAGACTC TAGATTCAGC TCTAAAAGAC 
ATCTATGATC ACATCCGCAC TGAGAAACGC AGGAGTAGCC TGTCAGAAAA CAGCATCCTT 
CATAGGTTGT TGGTGGAGTT GCTTCCGGAC ATTCCTGAGA GGAATTCCTC CCTGCATGCA 
CTGGGGAGAA ACAGTCCTAC AATTCAACCT CGCTCTTACC ACCCACAACC TGATGGCATG 
TCCAGTCAGG ACATTCACCA ATCCGAGTAC TGTCACCTGT CCCATAGTGC CTCTCACAAC 
CATATAGACA GCAACAACAA CCAGAGGAAC CACAACTTTA AATACTGCTA TCAAGACCAG 
GACACATCCA GAAATTACAC TTACCCTGAT GTGGGCCGGC AAACACCACA GAGCAGGAGA 
CAAACCCCTG ATGTCAGAGA GAAACTGCCA GCCAGAGCAA TATATGACTT CAAAGCACAG 
ACGGCCAAAG AGCTGACGTT CAAAAAAGGA GACACAGTAT ACATCAGTCG GCAGATAGAT 
AATAACTGGT ATGAAGGAGA ATACCATGGA CATGTAGGGA TATTCCCCAT CTCATATGTG 
GAGAAAATCC CTCCATCAGA GCGACATCTG CCAGCAAGAC CACCTCCTCC AGCCCAGAGC 
AAAGAAATTG GAGAAGCAGT TGCCCGTTAC AACTTTAATG CTGATACTAA TGTGGAACTT 
TCATTAAGAA AAGGAGAGCG TGTTGTATTG GTGCGGCAGG TGGACAAAAA CTGGTTCGAG 
GGAAAGATCC CAGGTACAAA CAAACAAGGA ATATTTCCAG TGTCGTATGT GGATGTCATT 
AAGAAGACTT CAGTACAAAA TACTGGACAA CCGCCTGGGC CTAGTATACC CACCAGCTTC 
TCCAGTGACA GACTGAACAG TAGGCCGTCA TCTACGCGTA ACCTCCACAA CTCTCCATCC 
CCAACTATCC GTCCACTCTC CTCATCTTCT CCCAGTCCAC AGAGAGCTGC ACACCTTCAA 
GCCATCACCA GCGAGTGGTT GGCTCTCACT CTGGGTCTGT CCCCTTCAGG AACCCCTGCC 
CCCACACCTC CTCCCTTCCC TTCCAATTTC CAGCCATACT ATGAAGCGTT TGACTCTGGC 
ATCTCGCCAT CTCCCGCCTC CTCCACACTG GGCCGCTCTC CAACACTCAC TCCTCGCGTC 
TCCACACCTG CACTCAAAGA GGGCCACTTC ATTCCCATCT CCTCCCCAAA GTCCTACATG 
TCCCCTGATC CCAGCCCGTC ACCTCAACCC TACCTAACCT CCGCCTCCTT CACTCCTTCA 
CCCACTTCAC CCACATTTGA CTCCAGGTCT GGCAGTGTCA TAGAAATTCT GTCCAGTCAC 
AAAGCAGATT TGCCTGTGAA GGAACTGCAG TTGTTCTCAG ATCCCAAAGA CAGATACAAG 
CCAGGCGAGA CAGACTCCCC TAAATTGGGC AGCCCTATTG ACTTAGTTGT TTTTGAAGGT 
CAAAAATCTC CATTGGATAT TCTGCCACCA AAAGACCTAG AGGATGATCT GTGTGAGGAG 
CTAGTGTCGA TTATAAAGGC CAGCCAATCA AAGGGTGCAT TCTTAGAAGA GGAGGATTTG 
TATCACCAAG AACCAGATAC TGTGGAAAAG CTACCCAGAC TCTTCATTGA GGAGGAGCAG 
AATGAAGACA ACAGCTTCTT AAATAAAACC CTGCCATCAA ATACATTCAC TCCACTCCAA 
CTTGCCCATA GTGAGGGTTC AGATATTGAG CAGGGTGAAG TTTCCCAGAA TGCCTCTCCT 
CGTCTCTCCC CTCTGTCCTC ACAGATGTCA TTGCCATTCA CATCTGCTAA ATACTCCCCC 
ACTTCCCCAT GCTCCACCCC ACCATTGCCT GGGGTTTCAC AGTCACCCCC TCACTTGGCA 
AAACTGTTCC CTCGGCAGGA CCTCCGCTCC CCAAAGGTCA AGCCTGTGTT AAGGCGTGAT 
GTTGTGGTTG TTGGTAAACC CCCTCGTAGC CCTGTGATGT CTAGGAGGTC CTGCGGATCA 
CCTGTTAGAG GTCAAAACTT TTCGCCATCT AATAGGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481184.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336859.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa148830
Clone ID Related Accession (Same CDS sequence)	XM_021481184.1
Accession Version	XM_021481184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCCCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAGAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GAAGAACCAA GTTTTTTGGA ACCTTTCCGG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336859.1
CDS	524..5146
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481184.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481184.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCA GGGTGAAGTT TCCCAGAATG CCTCTCCTCG TCTCTCCCCT 
CTGTCCTCAC AGATGTCATT GCCATTCACA TCTGCTAAAT ACTCCCCCAC TTCCCCATGC 
TCCACCCCAC CATTGCCTGG GGTTTCACAG TCACCCCCTC ACTTGGCAAA ACTGTTCCCT 
CGGCAGGACC TCCGCTCCCC AAAGGTCAAG CCTGTGTTAA GGCGTGATGT TGTGGTTGTT 
GGTAAACCCC CTCGTAGCCC TGTGATGTCT AGGAGGTCCT GCGGATCACC TGTTAGAGGT 
CAAAACTTTT CGCCATCTAA TAGGTCCCAA AGACAAGCAT ATGCTTGTGA TCCACTTCAA 
GGTGGAGGGG AACCATTTCA AGCACTGTAT AACTACATGC CACGGAATGA AGATGAGCTG 
GAGCTGAAGG AAGGTGATGT TGTTGATGTC ATGGAGAAGT GTGATGATGG ATGGTTTGTG 
GGAACGTCAA GAAGAACCAA GTTTTTTGGA ACCTTTCCGG GGAACTATGT GAAGAGGCTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359127.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214616.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

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CloneID	ODa54987
Clone ID Related Accession (Same CDS sequence)	XM_017359127.1 , XM_021481172.1
Accession Version	XM_017359127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATACCCTC 
AGTCTGATGC CCACACAGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGCGG TTCACACCAA TGCCCTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTCAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGGGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC GTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCGTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTAGATCCT 
CCTGCCCGAG CACCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGTC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCCAC AACTACTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCGT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCTT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTCACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GTCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATAAACT CTACTGTCAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGAAC CGGCAACAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC AGAAGGACGG 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAAGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCGGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ATGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGCCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAGAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCAGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTCGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCCA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACGCGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CTCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTAACCTCC 
GCCTCCTTCA CTCCTTCACC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCCCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAGAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GAAGAACCAA GTTTTTTGGA 
ACCTTTCCGG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214616.1
CDS	502..5874
Translation	MPGLCDIKTLQEGEIFNMNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359127.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359127.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATACCCTC 
AGTCTGATGC CCACACAGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGCGG TTCACACCAA TGCCCTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTCAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGGGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC GTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCGTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTAGATCCT 
CCTGCCCGAG CACCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGTC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCCAC AACTACTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCGT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCTT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTCACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GTCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATAAACT CTACTGTCAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGAAC CGGCAACAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC AGAAGGACGG 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAAGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCGGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ATGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGCCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAGAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCAGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTCGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCCA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACGCGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CTCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTAACCTCC 
GCCTCCTTCA CTCCTTCACC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCCCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAGAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GAAGAACCAA GTTTTTTGGA 
ACCTTTCCGG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481172.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336847.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

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CloneID	ODa54987
Clone ID Related Accession (Same CDS sequence)	XM_017359127.1 , XM_021481172.1
Accession Version	XM_021481172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATACCCTC 
AGTCTGATGC CCACACAGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGCGG TTCACACCAA TGCCCTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTCAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGGGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC GTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCGTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTAGATCCT 
CCTGCCCGAG CACCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGTC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCCAC AACTACTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCGT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCTT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTCACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GTCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATAAACT CTACTGTCAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGAAC CGGCAACAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC AGAAGGACGG 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAAGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCGGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ATGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGCCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAGAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCAGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTCGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCCA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACGCGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CTCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTAACCTCC 
GCCTCCTTCA CTCCTTCACC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCCCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAGAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GAAGAACCAA GTTTTTTGGA 
ACCTTTCCGG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336847.1
CDS	526..5898
Translation	MPGLCDIKTLQEGEIFNMNTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481172.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481172.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACTCTGAAT CACAATCCAG AGATTCAGAT TTGTGGAGGC CCAGTAGTAG CACTGATGGT 
CTAAGAAACG GTGACGTCTG CTCTTCCTCT CTGGCAGCTA AAGGTTACAG AAGTGTCAGG 
CCCATCTTTC AGGAAAAAAA GTCTCCCACA CCGGCACAAG AGCAAAATGT CCATACCCTC 
AGTCTGATGC CCACACAGGA CCAAATTTCA GGCTTTTCAC AGCAGTCACA CTTGCAACCT 
CCGTCAAGGG AGCTGGAGAG ACACGTAGCT TCCCTCTCCA TCCACATTCA CCCGAGGCCT 
CAGATATCGG GACCCCGCAC ACAGGCAATG TCTTTGAGAC CTCCCACCCC TCCTAAAAGG 
ATGGACCGGC AGGATCGCGG TTCACACCAA TGCCCTCCAC TGCCATCTGC AGCCCCGCCA 
GTGCCTGAGG TGCCAGTCCT CCTCAAACAG AGCTATCCTG GAACAGCACA GTCCCATAAC 
CGGCTCAGAC TCTCCCTCGA TTTCATCAGA CCTGAGACCT TTTCTCCAGC AGGCCCTCAC 
TCCCCCGACC GCTGCTCATC CTGTACTCAG AGCGAAGCAT CCACTGCAGT GCTGGAGGAG 
TTGAGGGGGT TTGGACTGGG CCCCGAGGGC GGCTCTCGTA CCCCTTCACC AACACTCAGC 
CAGATGAGTG TTGTCACAGA CAGCATGGGG TTACACTCCC GTGCAGCTAG CAACATTAAT 
GGCCAGATGA CTGTAAACGG TGGTCTGAGT GTGCCAGCAA GTCCTCGTAG TCACATCCAG 
AGACCCTTCT CTCCGTCAAC ATACCCTCCT CCTCCTTCAC TGAGTCCCAG TATTACAGCA 
ATGCAGCAGC AGGCTAGAAC CACTGCCTCC AGCACTCCAA TCTACGCCAA CGTAGATCCT 
CCTGCCCGAG CACCGCATAC TGACAGGAGA GAAACAGCAG GGAAAGCTCC GCACTATACT 
GGCATCGGTC CTGTGGATGA GTCTGGGATT CCCATAGCAA TACGGACGTC TGTTGACAGG 
CCAAAAGATT GGTACAAGAC CATGTTCAAA CAGATTCATG TGGTGCCTAA AACAGAGATC 
GAGTGGTCCG GGTCCCGAAC TGCCACAGAT CCTGTTACAA GTCCAGCCAC AACTACTTCC 
AAACAAGATA AGCAAGCTGC CCCTAATGCT GTCCAGGCTC ATCCTGCACC AAAAACCAGC 
ACCTACCGGC CCATAAGCAA GAGTGTTTCC GACAATGGCA TATATGCTTT CAGGCTGCCC 
GTCGCCTCTC CCCTGCCCAC ATCAACCTCC ACACAGCAGA GACCCATTGA AAGAGAGGCT 
CCACAGAGAG GCAGAAGCAC ACCAGACATG AATGAATGGG GGCCTCCTGA TAGAAAAGTG 
GACACACGGA AATACAGAGC AGAGCCAAGG AGCATCTTTG ACTATGAGCC TGGAAAATCA 
TCTGTTTTAG AACAGGAGAG AGCGAAAAGT AACTTAAGTC CAGAGGACAT AGATTTAGAG 
AATGAGCCGT GGTATAAGTT CTTTGCTGAA CTGGAGTTTG GGCGGCCGCC TCCAAAAAAA 
CTTCTGGATT ACAATCCAGA GAGCGCTCCT CGATTTCCAG TGGAGACCTC TCTTTATCAG 
CCATCCACTG ACAGGAGCTT TGAAAGGCCA TCAAGCTCTG CAAGTGATAA CAAGAAGAGG 
CGAAAATCCG AACCTGCTAC CACTCAGCCA AGAGCTCACA GCAGTGTGGG CACAACACAA 
ACATCTGTCA GACCACCAGA GCTGCCCAGG GGCAGCAGCA CCCAGAGGAA TCCCCTCACC 
AGCCCAAGCT CCCCCGCCAC CACTAGAACT AAAGGTGGGG ATGTGAGCAA TATGCACACA 
GCACATTCAA ACTGTCTAAG TCCAAACCAT TCTACCTCAG AACCTGTGTA TCAAAACTAT 
GGTGAAAATG AAGACAGTTT TATGGCTGTC CCAGCTGTCT CAGAAGGCTT CTCCAGCCTC 
CCAGAAGGCT GGAAACCAGC CCAAGAGAAT TCAGAAGACT GGATGATCGC AGAGGAGACC 
TCACCTAAGC TCAAATCTTG GAGTTGTGAT GACCTCCTGT CTGAGGGAAG GGGAAGAGAA 
GTGCAGACCC GTTGTGAAAG CACTGGCTCC TTGTTGCACA ATGGGGAACT AGAGACATCA 
CAATACTGTG ATGTCCAAGA GTCTGAGGAT CTTAGGGAGA GGACCCGCCA CCGCTCTGCC 
CACGATGCAC CAGGCTTTCT TAAACTCTAC AAGAAGATGC ATCACATTAA CAGGCAAGAG 
TTGATAAACT CTACTGTCAT CTGCTCTGTC AAATCCAGGA TCCGTAAGTA TGAGAGCGAA 
CAGCGAAAGG ATAGGCGAAC CGGCAACAAG GGCTGCAACG AAGAGGTGCC TAGAGACATG 
GTGCACAACA GGATTTCTGA ATTTGAGAGT CTGATTCAGA AGTCTAAATC CATGCCAAAC 
CTTGGGGAGG AGTGCCTAAT CAGAGGTTCT TCCAGGAGGA TCAACAGCCC TAAGCGTAGC 
CTCTCTATTG AGTCGTTGCT AGATGAAGAA CCACCAGCCA GGAATCCCCC AGAAGGACGG 
CCCCAATACC CCAAGATAAA CAACCATGTG CCAATTCACA TCCATGTCAC CAGTGACCAA 
TTACAAAACT CTGCCATGCA GAATGATTAT TCGGACAGTG AGCATGATGC TGTAGTGTCC 
GATCTTAGTG ACTTCATCCA GATTGAAGGG TCATCTTTTT GCAGTGAGAG GGAGTTTGAC 
TTGTGTTCAT TTGCCTCCTC CGAAAGTTTC TGTGGATCTG GACACCACCA TCATTTTCAC 
AGGCAACTTG TGAGCTCCTG CAAGGGCCGC TGCCCGGCTT CCTATACACG CTTCACCACC 
ATGATCAAGC ATGAGAGGGC CAAGCAGGAT CGGAGGCAAC ATTTGCGAGT AGAAGATTCT 
GAGTTTGGTC TCAGTAAGCT TGCATTCCTG GTCAGCCCAG TGCCTTTCCG TCGGAAGAAC 
CTGTCACCTT TGAGTTTTTC CCGAATGTCA AACTCCAAGA GCTATGTGTA CGAGACTCTA 
GATTCAGCTC TAAAAGACAT CTATGATCAC ATCCGCACTG AGAAACGCAG GAGTAGCCTG 
TCAGAAAACA GCATCCTTCA TAGGTTGTTG GTGGAGTTGC TTCCGGACAT TCCTGAGAGG 
AATTCCTCCC TGCATGCACT GGGGAGAAAC AGTCCTACAA TTCAACCTCG CTCTTACCAC 
CCACAACCTG ATGGCATGTC CAGTCAGGAC ATTCACCAAT CCGAGTACTG TCACCTGTCC 
CATAGTGCCT CTCACAACCA TATAGACAGC AACAACAACC AGAGGAACCA CAACTTTAAA 
TACTGCTATC AAGACCAGGA CACATCCAGA AATTACACTT ACCCTGATGT GGGCCGGCAA 
ACACCACAGA GCAGGAGACA AACCCCTGAT GTCAGAGAGA AACTGCCAGC CAGAGCAATA 
TATGACTTCA AAGCACAGAC GGCCAAAGAG CTGACGTTCA AAAAAGGAGA CACAGTATAC 
ATCAGTCGGC AGATAGATAA TAACTGGTAT GAAGGAGAAT ACCATGGACA TGTAGGGATA 
TTCCCCATCT CATATGTGGA GAAAATCCCT CCATCAGAGC GACATCTGCC AGCAAGACCA 
CCTCCTCCAG CCCAGAGCAA AGAAATTGGA GAAGCAGTTG CCCGTTACAA CTTTAATGCT 
GATACTAATG TGGAACTTTC ATTAAGAAAA GGAGAGCGTG TTGTATTGGT GCGGCAGGTG 
GACAAAAACT GGTTCGAGGG AAAGATCCCA GGTACAAACA AACAAGGAAT ATTTCCAGTG 
TCGTATGTGG ATGTCATTAA GAAGACTTCA GTACAAAATA CTGGACAACC GCCTGGGCCT 
AGTATACCCA CCAGCTTCTC CAGTGACAGA CTGAACAGTA GGCCGTCATC TACGCGTAAC 
CTCCACAACT CTCCATCCCC AACTATCCGT CCACTCTCCT CATCTTCTCC CAGTCCACAG 
AGAGCTGCAC ACCTTCAAGC CATCACCAGC GAGTGGTTGG CTCTCACTCT GGGTCTGTCC 
CCTTCAGGAA CCCCTGCCCC CACACCTCCT CCCTTCCCTT CCAATTTCCA GCCATACTAT 
GAAGCGTTTG ACTCTGGCAT CTCGCCATCT CCCGCCTCCT CCACACTGGG CCGCTCTCCA 
ACACTCACTC CTCGCGTCTC CACACCTGCA CTCAAAGAGG GCCACTTCAT TCCCATCTCC 
TCCCCAAAGT CCTACATGTC CCCTGATCCC AGCCCGTCAC CTCAACCCTA CCTAACCTCC 
GCCTCCTTCA CTCCTTCACC CACTTCACCC ACATTTGACT CCAGGTCTGG CAGTGTCATA 
GAAATTCTGT CCAGTCACAA AGCAGATTTG CCTGTGAAGG AACTGCAGTT GTTCTCAGAT 
CCCAAAGACA GATACAAGCC AGGCGAGACA GACTCCCCTA AATTGGGCAG CCCTATTGAC 
TTAGTTGTTT TTGAAGGTCA AAAATCTCCA TTGGATATTC TGCCACCAAA AGACCTAGAG 
GATGATCTGT GTGAGGAGCT AGTGTCGATT ATAAAGGCCA GCCAATCAAA GGGTGCATTC 
TTAGAAGAGG AGGATTTGTA TCACCAAGAA CCAGATACTG TGGAAAAGCT ACCCAGACTC 
TTCATTGAGG AGGAGCAGAA TGAAGACAAC AGCTTCTTAA ATAAAACCCT GCCATCAAAT 
ACATTCACTC CACTCCAACT TGCCCATAGT GAGGGTTCAG ATATTGAGCA GGGTGAAGTT 
TCCCAGAATG CCTCTCCTCG TCTCTCCCCT CTGTCCTCAC AGATGTCATT GCCATTCACA 
TCTGCTAAAT ACTCCCCCAC TTCCCCATGC TCCACCCCAC CATTGCCTGG GGTTTCACAG 
TCACCCCCTC ACTTGGCAAA ACTGTTCCCT CGGCAGGACC TCCGCTCCCC AAAGGTCAAG 
CCTGTGTTAA GGCGTGATGT TGTGGTTGTT GGTAAACCCC CTCGTAGCCC TGTGATGTCT 
AGGAGGTCCT GCGGATCACC TGTTAGAGGT CAAAACTTTT CGCCATCTAA TAGGTCCCAA 
AGACAAGCAT ATGCTTGTGA TCCACTTCAA GGTGGAGGGG AACCATTTCA AGCACTGTAT 
AACTACATGC CACGGAATGA AGATGAGCTG GAGCTGAAGG AAGGTGATGT TGTTGATGTC 
ATGGAGAAGT GTGATGATGG ATGGTTTGTG GGAACGTCAA GAAGAACCAA GTTTTTTGGA 
ACCTTTCCGG GGAACTATGT GAAGAGGCTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359128.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214617.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

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CloneID	ODa54988
Clone ID Related Accession (Same CDS sequence)	XM_017359128.1 , XM_021481173.1
Accession Version	XM_017359128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214617.1
CDS	502..5856
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359128.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359128.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481173.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336848.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

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CloneID	ODa54988
Clone ID Related Accession (Same CDS sequence)	XM_017359128.1 , XM_021481173.1
Accession Version	XM_021481173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336848.1
CDS	526..5880
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481173.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481173.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGTCCC AAAGACAAGC ATATGCTTGT 
GATCCACTTC AAGGTGGAGG GGAACCATTT CAAGCACTGT ATAACTACAT GCCACGGAAT 
GAAGATGAGC TGGAGCTGAA GGAAGGTGAT GTTGTTGATG TCATGGAGAA GTGTGATGAT 
GGATGGTTTG TGGGAACGTC AAGAAGAACC AAGTTTTTTG GAACCTTTCC GGGGAACTAT 
GTGAAGAGGC TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359129.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214618.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

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CloneID	ODa54989
Clone ID Related Accession (Same CDS sequence)	XM_017359129.1 , XM_021481175.1
Accession Version	XM_017359129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCTTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TCACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAGAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGTCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT AAACTCTACT GTCATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGAACCGGCA ACAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCAGAAG GACGGCCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AAGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCATGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGCC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAG AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC AGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTC GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCCACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACGC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCTCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTAA CCTCCGCCTC CTTCACTCCT TCACCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCCCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA GAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGAAGA 
ACCAAGTTTT TTGGAACCTT TCCGGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214618.1
CDS	502..5829
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERATSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359129.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359129.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCTTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TCACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAGAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGTCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT AAACTCTACT GTCATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGAACCGGCA ACAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCAGAAG GACGGCCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AAGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCATGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGCC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAG AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC AGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTC GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCCACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACGC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCTCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTAA CCTCCGCCTC CTTCACTCCT TCACCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCCCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA GAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGAAGA 
ACCAAGTTTT TTGGAACCTT TCCGGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481175.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336850.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

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CloneID	ODa54989
Clone ID Related Accession (Same CDS sequence)	XM_017359129.1 , XM_021481175.1
Accession Version	XM_021481175.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCTTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TCACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAGAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGTCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT AAACTCTACT GTCATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGAACCGGCA ACAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCAGAAG GACGGCCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AAGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCATGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGCC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAG AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC AGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTC GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCCACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACGC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCTCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTAA CCTCCGCCTC CTTCACTCCT TCACCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCCCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA GAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGAAGA 
ACCAAGTTTT TTGGAACCTT TCCGGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336850.1
CDS	526..5853
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERATSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481175.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481175.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
ACCTCTCTTT ATCAGCCATC CACTGACAGG AGCTTTGAAA GGCCATCAAG CTCTGCAAGT 
GATAACAAGA AGAGGCGAAA ATCCGAACCT GCTACCACTC AGCCAAGAGC TCACAGCAGT 
GTGGGCACAA CACAAACATC TGTCAGACCA CCAGAGCTGC CCAGGGGCAG CAGCACCCAG 
AGGAATCCCC TCACCAGCCC AAGCTCCCCC GCCACCACTA GAACTAAAGG TGGGGATGTG 
AGCAATATGC ACACAGCACA TTCAAACTGT CTAAGTCCAA ACCATTCTAC CTCAGAACCT 
GTGTATCAAA ACTATGGTGA AAATGAAGAC AGTTTTATGG CTGTCCCAGC TGTCTCAGAA 
GGCTTCTCCA GCCTCCCAGA AGGCTGGAAA CCAGCCCAAG AGAATTCAGA AGACTGGATG 
ATCGCAGAGG AGACCTCACC TAAGCTCAAA TCTTGGAGTT GTGATGACCT CCTGTCTGAG 
GGAAGGGGAA GAGAAGTGCA GACCCGTTGT GAAAGCACTG GCTCCTTGTT GCACAATGGG 
GAACTAGAGA CATCACAATA CTGTGATGTC CAAGAGTCTG AGGATCTTAG GGAGAGGACC 
CGCCACCGCT CTGCCCACGA TGCACCAGGC TTTCTTAAAC TCTACAAGAA GATGCATCAC 
ATTAACAGGC AAGAGTTGAT AAACTCTACT GTCATCTGCT CTGTCAAATC CAGGATCCGT 
AAGTATGAGA GCGAACAGCG AAAGGATAGG CGAACCGGCA ACAAGGGCTG CAACGAAGAG 
GTGCCTAGAG ACATGGTGCA CAACAGGATT TCTGAATTTG AGAGTCTGAT TCAGAAGTCT 
AAATCCATGC CAAACCTTGG GGAGGAGTGC CTAATCAGAG GTTCTTCCAG GAGGATCAAC 
AGCCCTAAGC GTAGCCTCTC TATTGAGTCG TTGCTAGATG AAGAACCACC AGCCAGGAAT 
CCCCCAGAAG GACGGCCCCA ATACCCCAAG ATAAACAACC ATGTGCCAAT TCACATCCAT 
GTCACCAGTG ACCAATTACA AAACTCTGCC ATGCAGAATG ATTATTCGGA CAGTGAGCAT 
GATGCTGTAG TGTCCGATCT TAGTGACTTC ATCCAGATTG AAGGGTCATC TTTTTGCAGT 
GAGAGGGAGT TTGACTTGTG TTCATTTGCC TCCTCCGAAA GTTTCTGTGG ATCTGGACAC 
CACCATCATT TTCACAGGCA ACTTGTGAGC TCCTGCAAGG GCCGCTGCCC GGCTTCCTAT 
ACACGCTTCA CCACCATGAT CAAGCATGAG AGGGCCAAGC AGGATCGGAG GCAACATTTG 
CGAGTAGAAG ATTCTGAGTT TGGTCTCAGT AAGCTTGCAT TCCTGGTCAG CCCAGTGCCT 
TTCCGTCGGA AGAACCTGTC ACCTTTGAGT TTTTCCCGAA TGTCAAACTC CAAGAGCTAT 
GTGTACGAGA CTCTAGATTC AGCTCTAAAA GACATCTATG ATCACATCCG CACTGAGAAA 
CGCAGGAGTA GCCTGTCAGA AAACAGCATC CTTCATAGGT TGTTGGTGGA GTTGCTTCCG 
GACATTCCTG AGAGGAATTC CTCCCTGCAT GCACTGGGGA GAAACAGTCC TACAATTCAA 
CCTCGCTCTT ACCACCCACA ACCTGATGGC ATGTCCAGTC AGGACATTCA CCAATCCGAG 
TACTGTCACC TGTCCCATAG TGCCTCTCAC AACCATATAG ACAGCAACAA CAACCAGAGG 
AACCACAACT TTAAATACTG CTATCAAGAC CAGGACACAT CCAGAAATTA CACTTACCCT 
GATGTGGGCC GGCAAACACC ACAGAGCAGG AGACAAACCC CTGATGTCAG AGAGAAACTG 
CCAGCCAGAG CAATATATGA CTTCAAAGCA CAGACGGCCA AAGAGCTGAC GTTCAAAAAA 
GGAGACACAG TATACATCAG TCGGCAGATA GATAATAACT GGTATGAAGG AGAATACCAT 
GGACATGTAG GGATATTCCC CATCTCATAT GTGGAGAAAA TCCCTCCATC AGAGCGACAT 
CTGCCAGCAA GACCACCTCC TCCAGCCCAG AGCAAAGAAA TTGGAGAAGC AGTTGCCCGT 
TACAACTTTA ATGCTGATAC TAATGTGGAA CTTTCATTAA GAAAAGGAGA GCGTGTTGTA 
TTGGTGCGGC AGGTGGACAA AAACTGGTTC GAGGGAAAGA TCCCAGGTAC AAACAAACAA 
GGAATATTTC CAGTGTCGTA TGTGGATGTC ATTAAGAAGA CTTCAGTACA AAATACTGGA 
CAACCGCCTG GGCCTAGTAT ACCCACCAGC TTCTCCAGTG ACAGACTGAA CAGTAGGCCG 
TCATCTACGC GTAACCTCCA CAACTCTCCA TCCCCAACTA TCCGTCCACT CTCCTCATCT 
TCTCCCAGTC CACAGAGAGC TGCACACCTT CAAGCCATCA CCAGCGAGTG GTTGGCTCTC 
ACTCTGGGTC TGTCCCCTTC AGGAACCCCT GCCCCCACAC CTCCTCCCTT CCCTTCCAAT 
TTCCAGCCAT ACTATGAAGC GTTTGACTCT GGCATCTCGC CATCTCCCGC CTCCTCCACA 
CTGGGCCGCT CTCCAACACT CACTCCTCGC GTCTCCACAC CTGCACTCAA AGAGGGCCAC 
TTCATTCCCA TCTCCTCCCC AAAGTCCTAC ATGTCCCCTG ATCCCAGCCC GTCACCTCAA 
CCCTACCTAA CCTCCGCCTC CTTCACTCCT TCACCCACTT CACCCACATT TGACTCCAGG 
TCTGGCAGTG TCATAGAAAT TCTGTCCAGT CACAAAGCAG ATTTGCCTGT GAAGGAACTG 
CAGTTGTTCT CAGATCCCAA AGACAGATAC AAGCCAGGCG AGACAGACTC CCCTAAATTG 
GGCAGCCCTA TTGACTTAGT TGTTTTTGAA GGTCAAAAAT CTCCATTGGA TATTCTGCCA 
CCAAAAGACC TAGAGGATGA TCTGTGTGAG GAGCTAGTGT CGATTATAAA GGCCAGCCAA 
TCAAAGGGTG CATTCTTAGA AGAGGAGGAT TTGTATCACC AAGAACCAGA TACTGTGGAA 
AAGCTACCCA GACTCTTCAT TGAGGAGGAG CAGAATGAAG ACAACAGCTT CTTAAATAAA 
ACCCTGCCAT CAAATACATT CACTCCACTC CAACTTGCCC ATAGTGAGGG TTCAGATATT 
GAGCAGGGTG AAGTTTCCCA GAATGCCTCT CCTCGTCTCT CCCCTCTGTC CTCACAGATG 
TCATTGCCAT TCACATCTGC TAAATACTCC CCCACTTCCC CATGCTCCAC CCCACCATTG 
CCTGGGGTTT CACAGTCACC CCCTCACTTG GCAAAACTGT TCCCTCGGCA GGACCTCCGC 
TCCCCAAAGG TCAAGCCTGT GTTAAGGCGT GATGTTGTGG TTGTTGGTAA ACCCCCTCGT 
AGCCCTGTGA TGTCTAGGAG GTCCTGCGGA TCACCTGTTA GAGGTCAAAA CTTTTCGCCA 
TCTAATAGGT CCCAAAGACA AGCATATGCT TGTGATCCAC TTCAAGGTGG AGGGGAACCA 
TTTCAAGCAC TGTATAACTA CATGCCACGG AATGAAGATG AGCTGGAGCT GAAGGAAGGT 
GATGTTGTTG ATGTCATGGA GAAGTGTGAT GATGGATGGT TTGTGGGAAC GTCAAGAAGA 
ACCAAGTTTT TTGGAACCTT TCCGGGGAAC TATGTGAAGA GGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_017359126.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214615.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

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CloneID	ODa44079
Clone ID Related Accession (Same CDS sequence)	XM_017359126.1 , XM_021481169.1 , XM_009291157.2 , XM_009291157.3
Accession Version	XM_017359126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214615.1
CDS	481..5949
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_017359126.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359126.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481169.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336844.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

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CloneID	ODa44079
Clone ID Related Accession (Same CDS sequence)	XM_017359126.1 , XM_021481169.1 , XM_009291157.2 , XM_009291157.3
Accession Version	XM_021481169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336844.1
CDS	503..5971
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481169.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481169.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_009291157.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289432.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

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CloneID	ODa44079
Clone ID Related Accession (Same CDS sequence)	XM_017359126.1 , XM_021481169.1 , XM_009291157.2 , XM_009291157.3
Accession Version	XM_009291157.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289432.1
CDS	551..6019
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_009291157.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291157.2

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_009291157.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289432.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

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CloneID	ODa44079
Clone ID Related Accession (Same CDS sequence)	XM_017359126.1 , XM_021481169.1 , XM_009291157.2 , XM_009291157.3
Accession Version	XM_009291157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289432.1
CDS	527..5995
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_009291157.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291157.3

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGCC GTCATCTACG CGTAACCTCC ACAACTCTCC ATCCCCAACT 
ATCCGTCCAC TCTCCTCATC TTCTCCCAGT CCACAGAGAG CTGCACACCT TCAAGCCATC 
ACCAGCGAGT GGTTGGCTCT CACTCTGGGT CTGTCCCCTT CAGGAACCCC TGCCCCCACA 
CCTCCTCCCT TCCCTTCCAA TTTCCAGCCA TACTATGAAG CGTTTGACTC TGGCATCTCG 
CCATCTCCCG CCTCCTCCAC ACTGGGCCGC TCTCCAACAC TCACTCCTCG CGTCTCCACA 
CCTGCACTCA AAGAGGGCCA CTTCATTCCC ATCTCCTCCC CAAAGTCCTA CATGTCCCCT 
GATCCCAGCC CGTCACCTCA ACCCTACCTA ACCTCCGCCT CCTTCACTCC TTCACCCACT 
TCACCCACAT TTGACTCCAG GTCTGGCAGT GTCATAGAAA TTCTGTCCAG TCACAAAGCA 
GATTTGCCTG TGAAGGAACT GCAGTTGTTC TCAGATCCCA AAGACAGATA CAAGCCAGGC 
GAGACAGACT CCCCTAAATT GGGCAGCCCT ATTGACTTAG TTGTTTTTGA AGGTCAAAAA 
TCTCCATTGG ATATTCTGCC ACCAAAAGAC CTAGAGGATG ATCTGTGTGA GGAGCTAGTG 
TCGATTATAA AGGCCAGCCA ATCAAAGGGT GCATTCTTAG AAGAGGAGGA TTTGTATCAC 
CAAGAACCAG ATACTGTGGA AAAGCTACCC AGACTCTTCA TTGAGGAGGA GCAGAATGAA 
GACAACAGCT TCTTAAATAA AACCCTGCCA TCAAATACAT TCACTCCACT CCAACTTGCC 
CATAGTGAGG GTTCAGATAT TGAGCAGGGT GAAGTTTCCC AGAATGCCTC TCCTCGTCTC 
TCCCCTCTGT CCTCACAGAT GTCATTGCCA TTCACATCTG CTAAATACTC CCCCACTTCC 
CCATGCTCCA CCCCACCATT GCCTGGGGTT TCACAGTCAC CCCCTCACTT GGCAAAACTG 
TTCCCTCGGC AGGACCTCCG CTCCCCAAAG GTCAAGCCTG TGTTAAGGCG TGATGTTGTG 
GTTGTTGGTA AACCCCCTCG TAGCCCTGTG ATGTCTAGGA GGTCCTGCGG ATCACCTGTT 
AGAGGTCAAA ACTTTTCGCC ATCTAATAGG TCCCAAAGAC AAGCATATGC TTGTGATCCA 
CTTCAAGGTG GAGGGGAACC ATTTCAAGCA CTGTATAACT ACATGCCACG GAATGAAGAT 
GAGCTGGAGC TGAAGGAAGG TGATGTTGTT GATGTCATGG AGAAGTGTGA TGATGGATGG 
TTTGTGGGAA CGTCAAGAAG AACCAAGTTT TTTGGAACCT TTCCGGGGAA CTATGTGAAG 
AGGCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_009291158.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289433.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

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CloneID	ODa44080
Clone ID Related Accession (Same CDS sequence)	XM_009291158.2 , XM_021481182.1
Accession Version	XM_009291158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289433.1
CDS	502..5403
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_009291158.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291158.2

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481182.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336857.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.

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CloneID	ODa44080
Clone ID Related Accession (Same CDS sequence)	XM_009291158.2 , XM_021481182.1
Accession Version	XM_021481182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336857.1
CDS	524..5425
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRPSSTRNLHNSPSPTIRPLSSSSPSPQRAAHLQAITSEWLALTLGLSPSGTPAPTPPPFPSNFQPYYEAFDSGISPSPASSTLGRSPTLTPRVSTPALKEGHFIPISSPKSYMSPDPSPSPQPYLTSASFTPSPTSPTFDSRSGSVIEILSSHKADLPVKELQLFSDPKDRYKPGETDSPKLGSPIDLVVFEGQKSPLDILPPKDLEDDLCEELVSIIKASQSKGAFLEEEDLYHQEPDTVEKLPRLFIEEEQNEDNSFLNKTLPSNTFTPLQLAHSEGSDIEQGEVSQNASPRLSPLSSQMSLPFTSAKYSPTSPCSTPPLPGVSQSPPHLAKLFPRQDLRSPKVKPVLRRDVVVVGKPPRSPVMSRRSCGSPVRGQNFSPSNRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481182.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481182.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG GCCAGATGAC TGTAAACGGT GGTCTGAGTG TGCCAGCAAG TCCTCGTAGT 
CACATCCAGA GACCCTTCTC TCCGTCAACA TACCCTCCTC CTCCTTCACT GAGTCCCAGT 
ATTACAGCAA TGCAGCAGCA GGCTAGAACC ACTGCCTCCA GCACTCCAAT CTACGCCAAC 
GTAGATCCTC CTGCCCGAGC ACCGCATACT GACAGGAGAG AAACAGCAGG GAAAGCTCCG 
CACTATACTG GCATCGGTCC TGTGGATGAG TCTGGGATTC CCATAGCAAT ACGGACGTCT 
GTTGACAGGC CAAAAGATTG GTACAAGACC ATGTTCAAAC AGATTCATGT GGTGCCTAAA 
ACAGAGATCG AGTGGTCCGG GTCCCGAACT GCCACAGATC CTGTTACAAG TCCAGCCACA 
ACTACTTCCA AACAAGATAA GCAAGCTGCC CCTAATGCTG TCCAGGCTCA TCCTGCACCA 
AAAACCAGCA CCTACCGGCC CATAAGCAAG AGTGTTTCCG ACAATGGCAT ATATGCTTTC 
AGGCTGCCCG TCGCCTCTCC CCTGCCCACA TCAACCTCCA CACAGCAGAG ACCCATTGAA 
AGAGAGGCTC CACAGAGAGG CAGAAGCACA CCAGACATGA ATGAATGGGG GCCTCCTGAT 
AGAAAAGTGG ACACACGGAA ATACAGAGCA GAGCCAAGGA GCATCTTTGA CTATGAGCCT 
GGAAAATCAT CTGTTTTAGA ACAGGAGAGA GCGAAAAGTA ACTTAAGTCC AGAGGACATA 
GATTTAGAGA ATGAGCCGTG GTATAAGTTC TTTGCTGAAC TGGAGTTTGG GCGGCCGCCT 
CCAAAAAAAC TTCTGGATTA CAATCCAGAG AGCGCTCCTC GATTTCCAGT GGAGACCTCT 
CTTTATCAGC CATCCACTGA CAGGAGCTTT GAAAGGCCAT CAAGCTCTGC AAGTGATAAC 
AAGAAGAGGC GAAAATCCGA ACCTGCTACC ACTCAGCCAA GAGCTCACAG CAGTGTGGGC 
ACAACACAAA CATCTGTCAG ACCACCAGAG CTGCCCAGGG GCAGCAGCAC CCAGAGGAAT 
CCCCTCACCA GCCCAAGCTC CCCCGCCACC ACTAGAACTA AAGGTGGGGA TGTGAGCAAT 
ATGCACACAG CACATTCAAA CTGTCTAAGT CCAAACCATT CTACCTCAGA ACCTGTGTAT 
CAAAACTATG GTGAAAATGA AGACAGTTTT ATGGCTGTCC CAGCTGTCTC AGAAGGCTTC 
TCCAGCCTCC CAGAAGGCTG GAAACCAGCC CAAGAGAATT CAGAAGACTG GATGATCGCA 
GAGGAGACCT CACCTAAGCT CAAATCTTGG AGTTGTGATG ACCTCCTGTC TGAGGGAAGG 
GGAAGAGAAG TGCAGACCCG TTGTGAAAGC ACTGGCTCCT TGTTGCACAA TGGGGAACTA 
GAGACATCAC AATACTGTGA TGTCCAAGAG TCTGAGGATC TTAGGGAGAG GACCCGCCAC 
CGCTCTGCCC ACGATGCACC AGGCTTTCTT AAACTCTACA AGAAGATGCA TCACATTAAC 
AGGCAAGAGT TGATAAACTC TACTGTCATC TGCTCTGTCA AATCCAGGAT CCGTAAGTAT 
GAGAGCGAAC AGCGAAAGGA TAGGCGAACC GGCAACAAGG GCTGCAACGA AGAGGTGCCT 
AGAGACATGG TGCACAACAG GATTTCTGAA TTTGAGAGTC TGATTCAGAA GTCTAAATCC 
ATGCCAAACC TTGGGGAGGA GTGCCTAATC AGAGGTTCTT CCAGGAGGAT CAACAGCCCT 
AAGCGTAGCC TCTCTATTGA GTCGTTGCTA GATGAAGAAC CACCAGCCAG GAATCCCCCA 
GAAGGACGGC CCCAATACCC CAAGATAAAC AACCATGTGC CAATTCACAT CCATGTCACC 
AGTGACCAAT TACAAAACTC TGCCATGCAG AATGATTATT CGGACAGTGA GCATGATGCT 
GTAGTGTCCG ATCTTAGTGA CTTCATCCAG ATTGAAGGGT CATCTTTTTG CAGTGAGAGG 
GAGTTTGACT TGTGTTCATT TGCCTCCTCC GAAAGTTTCT GTGGATCTGG ACACCACCAT 
CATTTTCACA GGCAACTTGT GAGCTCCTGC AAGGGCCGCT GCCCGGCTTC CTATACACGC 
TTCACCACCA TGATCAAGCA TGAGAGGGCC AAGCAGGATC GGAGGCAACA TTTGCGAGTA 
GAAGATTCTG AGTTTGGTCT CAGTAAGCTT GCATTCCTGG TCAGCCCAGT GCCTTTCCGT 
CGGAAGAACC TGTCACCTTT GAGTTTTTCC CGAATGTCAA ACTCCAAGAG CTATGTGTAC 
GAGACTCTAG ATTCAGCTCT AAAAGACATC TATGATCACA TCCGCACTGA GAAACGCAGG 
AGTAGCCTGT CAGAAAACAG CATCCTTCAT AGGTTGTTGG TGGAGTTGCT TCCGGACATT 
CCTGAGAGGA ATTCCTCCCT GCATGCACTG GGGAGAAACA GTCCTACAAT TCAACCTCGC 
TCTTACCACC CACAACCTGA TGGCATGTCC AGTCAGGACA TTCACCAATC CGAGTACTGT 
CACCTGTCCC ATAGTGCCTC TCACAACCAT ATAGACAGCA ACAACAACCA GAGGAACCAC 
AACTTTAAAT ACTGCTATCA AGACCAGGAC ACATCCAGAA ATTACACTTA CCCTGATGTG 
GGCCGGCAAA CACCACAGAG CAGGAGACAA ACCCCTGATG TCAGAGAGAA ACTGCCAGCC 
AGAGCAATAT ATGACTTCAA AGCACAGACG GCCAAAGAGC TGACGTTCAA AAAAGGAGAC 
ACAGTATACA TCAGTCGGCA GATAGATAAT AACTGGTATG AAGGAGAATA CCATGGACAT 
GTAGGGATAT TCCCCATCTC ATATGTGGAG AAAATCCCTC CATCAGAGCG ACATCTGCCA 
GCAAGACCAC CTCCTCCAGC CCAGAGCAAA GAAATTGGAG AAGCAGTTGC CCGTTACAAC 
TTTAATGCTG ATACTAATGT GGAACTTTCA TTAAGAAAAG GAGAGCGTGT TGTATTGGTG 
CGGCAGGTGG ACAAAAACTG GTTCGAGGGA AAGATCCCAG GTACAAACAA ACAAGGAATA 
TTTCCAGTGT CGTATGTGGA TGTCATTAAG AAGACTTCAG TACAAAATAC TGGACAACCG 
CCTGGGCCTA GTATACCCAC CAGCTTCTCC AGTGACAGAC TGAACAGTAG GCCGTCATCT 
ACGCGTAACC TCCACAACTC TCCATCCCCA ACTATCCGTC CACTCTCCTC ATCTTCTCCC 
AGTCCACAGA GAGCTGCACA CCTTCAAGCC ATCACCAGCG AGTGGTTGGC TCTCACTCTG 
GGTCTGTCCC CTTCAGGAAC CCCTGCCCCC ACACCTCCTC CCTTCCCTTC CAATTTCCAG 
CCATACTATG AAGCGTTTGA CTCTGGCATC TCGCCATCTC CCGCCTCCTC CACACTGGGC 
CGCTCTCCAA CACTCACTCC TCGCGTCTCC ACACCTGCAC TCAAAGAGGG CCACTTCATT 
CCCATCTCCT CCCCAAAGTC CTACATGTCC CCTGATCCCA GCCCGTCACC TCAACCCTAC 
CTAACCTCCG CCTCCTTCAC TCCTTCACCC ACTTCACCCA CATTTGACTC CAGGTCTGGC 
AGTGTCATAG AAATTCTGTC CAGTCACAAA GCAGATTTGC CTGTGAAGGA ACTGCAGTTG 
TTCTCAGATC CCAAAGACAG ATACAAGCCA GGCGAGACAG ACTCCCCTAA ATTGGGCAGC 
CCTATTGACT TAGTTGTTTT TGAAGGTCAA AAATCTCCAT TGGATATTCT GCCACCAAAA 
GACCTAGAGG ATGATCTGTG TGAGGAGCTA GTGTCGATTA TAAAGGCCAG CCAATCAAAG 
GGTGCATTCT TAGAAGAGGA GGATTTGTAT CACCAAGAAC CAGATACTGT GGAAAAGCTA 
CCCAGACTCT TCATTGAGGA GGAGCAGAAT GAAGACAACA GCTTCTTAAA TAAAACCCTG 
CCATCAAATA CATTCACTCC ACTCCAACTT GCCCATAGTG AGGGTTCAGA TATTGAGCAG 
GGTGAAGTTT CCCAGAATGC CTCTCCTCGT CTCTCCCCTC TGTCCTCACA GATGTCATTG 
CCATTCACAT CTGCTAAATA CTCCCCCACT TCCCCATGCT CCACCCCACC ATTGCCTGGG 
GTTTCACAGT CACCCCCTCA CTTGGCAAAA CTGTTCCCTC GGCAGGACCT CCGCTCCCCA 
AAGGTCAAGC CTGTGTTAAG GCGTGATGTT GTGGTTGTTG GTAAACCCCC TCGTAGCCCT 
GTGATGTCTA GGAGGTCCTG CGGATCACCT GTTAGAGGTC AAAACTTTTC GCCATCTAAT 
AGGTCCCAAA GACAAGCATA TGCTTGTGAT CCACTTCAAG GTGGAGGGGA ACCATTTCAA 
GCACTGTATA ACTACATGCC ACGGAATGAA GATGAGCTGG AGCTGAAGGA AGGTGATGTT 
GTTGATGTCA TGGAGAAGTG TGATGATGGA TGGTTTGTGG GAACGTCAAG AAGAACCAAG 
TTTTTTGGAA CCTTTCCGGG GAACTATGTG AAGAGGCTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_009291160.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289435.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

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CloneID	ODa44081
Clone ID Related Accession (Same CDS sequence)	XM_009291160.2 , XM_021481185.1
Accession Version	XM_009291160.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAG AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGAAGAA CCAAGTTTTT TGGAACCTTT CCGGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289435.1
CDS	502..4818
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_009291160.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291160.2

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAG AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGAAGAA CCAAGTTTTT TGGAACCTTT CCGGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sorbs2b ( XM_021481185.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336860.1 Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.

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CloneID	ODa44081
Clone ID Related Accession (Same CDS sequence)	XM_009291160.2 , XM_021481185.1
Accession Version	XM_021481185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sorbin and SH3 domain-containing protein 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAG AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGAAGAA CCAAGTTTTT TGGAACCTTT CCGGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336860.1
CDS	522..4838
Translation	MPGLCDIKTLQEGEIFNMNTDSGGQIRKCATLSLTLTPMKRIQSSPNLYSLTDSESQSRDSDLWRPSSSTDGLRNGDVCSSSLAAKGYRSVRPIFQEKKSPTPAQEQNVHTLSLMPTQDQISGFSQQSHLQPPSRELERHVASLSIHIHPRPQISGPRTQAMSLRPPTPPKRMDRQDRGSHQCPPLPSAAPPVPEVPVLLKQSYPGTAQSHNRLRLSLDFIRPETFSPAGPHSPDRCSSCTQSEASTAVLEELRGFGLGPEGGSRTPSPTLSQMSVVTDSMGLHSRAASNINGQMTVNGGLSVPASPRSHIQRPFSPSTYPPPPSLSPSITAMQQQARTTASSTPIYANVDPPARAPHTDRRETAGKAPHYTGIGPVDESGIPIAIRTSVDRPKDWYKTMFKQIHVVPKTEIEWSGSRTATDPVTSPATTTSKQDKQAAPNAVQAHPAPKTSTYRPISKSVSDNGIYAFRLPVASPLPTSTSTQQRPIEREAPQRGRSTPDMNEWGPPDRKVDTRKYRAEPRSIFDYEPGKSSVLEQERAKSNLSPEDIDLENEPWYKFFAELEFGRPPPKKLLDYNPESAPRFPVETSLYQPSTDRSFERPSSSASDNKKRRKSEPATTQPRAHSSVGTTQTSVRPPELPRGSSTQRNPLTSPSSPATTRTKGGDVSNMHTAHSNCLSPNHSTSEPVYQNYGENEDSFMAVPAVSEGFSSLPEGWKPAQENSEDWMIAEETSPKLKSWSCDDLLSEGRGREVQTRCESTGSLLHNGELETSQYCDVQESEDLRERTRHRSAHDAPGFLKLYKKMHHINRQELINSTVICSVKSRIRKYESEQRKDRRTGNKGCNEEVPRDMVHNRISEFESLIQKSKSMPNLGEECLIRGSSRRINSPKRSLSIESLLDEEPPARNPPEGRPQYPKINNHVPIHIHVTSDQLQNSAMQNDYSDSEHDAVVSDLSDFIQIEGSSFCSEREFDLCSFASSESFCGSGHHHHFHRQLVSSCKGRCPASYTRFTTMIKHERAKQDRRQHLRVEDSEFGLSKLAFLVSPVPFRRKNLSPLSFSRMSNSKSYVYETLDSALKDIYDHIRTEKRRSSLSENSILHRLLVELLPDIPERNSSLHALGRNSPTIQPRSYHPQPDGMSSQDIHQSEYCHLSHSASHNHIDSNNNQRNHNFKYCYQDQDTSRNYTYPDVGRQTPQSRRQTPDVREKLPARAIYDFKAQTAKELTFKKGDTVYISRQIDNNWYEGEYHGHVGIFPISYVEKIPPSERHLPARPPPPAQSKEIGEAVARYNFNADTNVELSLRKGERVVLVRQVDKNWFEGKIPGTNKQGIFPVSYVDVIKKTSVQNTGQPPGPSIPTSFSSDRLNSRSQRQAYACDPLQGGGEPFQALYNYMPRNEDELELKEGDVVDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

Target ORF information:

RefSeq Version	XM_021481185.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sorbin and SH3 domain containing 2b (sorbs2b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481185.1

ORF Insert Sequence:

ATGCCTGGAC TGTGTGATAT CAAGACACTG CAAGAAGGAG AGATCTTCAA TATGAACACA 
GACAGTGGAG GACAAATCCG CAAATGTGCC ACGCTTTCAC TCACTTTAAC CCCCATGAAG 
AGAATCCAGA GCTCGCCAAA CCTATACTCA CTCACAGACT CTGAATCACA ATCCAGAGAT 
TCAGATTTGT GGAGGCCCAG TAGTAGCACT GATGGTCTAA GAAACGGTGA CGTCTGCTCT 
TCCTCTCTGG CAGCTAAAGG TTACAGAAGT GTCAGGCCCA TCTTTCAGGA AAAAAAGTCT 
CCCACACCGG CACAAGAGCA AAATGTCCAT ACCCTCAGTC TGATGCCCAC ACAGGACCAA 
ATTTCAGGCT TTTCACAGCA GTCACACTTG CAACCTCCGT CAAGGGAGCT GGAGAGACAC 
GTAGCTTCCC TCTCCATCCA CATTCACCCG AGGCCTCAGA TATCGGGACC CCGCACACAG 
GCAATGTCTT TGAGACCTCC CACCCCTCCT AAAAGGATGG ACCGGCAGGA TCGCGGTTCA 
CACCAATGCC CTCCACTGCC ATCTGCAGCC CCGCCAGTGC CTGAGGTGCC AGTCCTCCTC 
AAACAGAGCT ATCCTGGAAC AGCACAGTCC CATAACCGGC TCAGACTCTC CCTCGATTTC 
ATCAGACCTG AGACCTTTTC TCCAGCAGGC CCTCACTCCC CCGACCGCTG CTCATCCTGT 
ACTCAGAGCG AAGCATCCAC TGCAGTGCTG GAGGAGTTGA GGGGGTTTGG ACTGGGCCCC 
GAGGGCGGCT CTCGTACCCC TTCACCAACA CTCAGCCAGA TGAGTGTTGT CACAGACAGC 
ATGGGGTTAC ACTCCCGTGC AGCTAGCAAC ATTAATGGCC AGATGACTGT AAACGGTGGT 
CTGAGTGTGC CAGCAAGTCC TCGTAGTCAC ATCCAGAGAC CCTTCTCTCC GTCAACATAC 
CCTCCTCCTC CTTCACTGAG TCCCAGTATT ACAGCAATGC AGCAGCAGGC TAGAACCACT 
GCCTCCAGCA CTCCAATCTA CGCCAACGTA GATCCTCCTG CCCGAGCACC GCATACTGAC 
AGGAGAGAAA CAGCAGGGAA AGCTCCGCAC TATACTGGCA TCGGTCCTGT GGATGAGTCT 
GGGATTCCCA TAGCAATACG GACGTCTGTT GACAGGCCAA AAGATTGGTA CAAGACCATG 
TTCAAACAGA TTCATGTGGT GCCTAAAACA GAGATCGAGT GGTCCGGGTC CCGAACTGCC 
ACAGATCCTG TTACAAGTCC AGCCACAACT ACTTCCAAAC AAGATAAGCA AGCTGCCCCT 
AATGCTGTCC AGGCTCATCC TGCACCAAAA ACCAGCACCT ACCGGCCCAT AAGCAAGAGT 
GTTTCCGACA ATGGCATATA TGCTTTCAGG CTGCCCGTCG CCTCTCCCCT GCCCACATCA 
ACCTCCACAC AGCAGAGACC CATTGAAAGA GAGGCTCCAC AGAGAGGCAG AAGCACACCA 
GACATGAATG AATGGGGGCC TCCTGATAGA AAAGTGGACA CACGGAAATA CAGAGCAGAG 
CCAAGGAGCA TCTTTGACTA TGAGCCTGGA AAATCATCTG TTTTAGAACA GGAGAGAGCG 
AAAAGTAACT TAAGTCCAGA GGACATAGAT TTAGAGAATG AGCCGTGGTA TAAGTTCTTT 
GCTGAACTGG AGTTTGGGCG GCCGCCTCCA AAAAAACTTC TGGATTACAA TCCAGAGAGC 
GCTCCTCGAT TTCCAGTGGA GACCTCTCTT TATCAGCCAT CCACTGACAG GAGCTTTGAA 
AGGCCATCAA GCTCTGCAAG TGATAACAAG AAGAGGCGAA AATCCGAACC TGCTACCACT 
CAGCCAAGAG CTCACAGCAG TGTGGGCACA ACACAAACAT CTGTCAGACC ACCAGAGCTG 
CCCAGGGGCA GCAGCACCCA GAGGAATCCC CTCACCAGCC CAAGCTCCCC CGCCACCACT 
AGAACTAAAG GTGGGGATGT GAGCAATATG CACACAGCAC ATTCAAACTG TCTAAGTCCA 
AACCATTCTA CCTCAGAACC TGTGTATCAA AACTATGGTG AAAATGAAGA CAGTTTTATG 
GCTGTCCCAG CTGTCTCAGA AGGCTTCTCC AGCCTCCCAG AAGGCTGGAA ACCAGCCCAA 
GAGAATTCAG AAGACTGGAT GATCGCAGAG GAGACCTCAC CTAAGCTCAA ATCTTGGAGT 
TGTGATGACC TCCTGTCTGA GGGAAGGGGA AGAGAAGTGC AGACCCGTTG TGAAAGCACT 
GGCTCCTTGT TGCACAATGG GGAACTAGAG ACATCACAAT ACTGTGATGT CCAAGAGTCT 
GAGGATCTTA GGGAGAGGAC CCGCCACCGC TCTGCCCACG ATGCACCAGG CTTTCTTAAA 
CTCTACAAGA AGATGCATCA CATTAACAGG CAAGAGTTGA TAAACTCTAC TGTCATCTGC 
TCTGTCAAAT CCAGGATCCG TAAGTATGAG AGCGAACAGC GAAAGGATAG GCGAACCGGC 
AACAAGGGCT GCAACGAAGA GGTGCCTAGA GACATGGTGC ACAACAGGAT TTCTGAATTT 
GAGAGTCTGA TTCAGAAGTC TAAATCCATG CCAAACCTTG GGGAGGAGTG CCTAATCAGA 
GGTTCTTCCA GGAGGATCAA CAGCCCTAAG CGTAGCCTCT CTATTGAGTC GTTGCTAGAT 
GAAGAACCAC CAGCCAGGAA TCCCCCAGAA GGACGGCCCC AATACCCCAA GATAAACAAC 
CATGTGCCAA TTCACATCCA TGTCACCAGT GACCAATTAC AAAACTCTGC CATGCAGAAT 
GATTATTCGG ACAGTGAGCA TGATGCTGTA GTGTCCGATC TTAGTGACTT CATCCAGATT 
GAAGGGTCAT CTTTTTGCAG TGAGAGGGAG TTTGACTTGT GTTCATTTGC CTCCTCCGAA 
AGTTTCTGTG GATCTGGACA CCACCATCAT TTTCACAGGC AACTTGTGAG CTCCTGCAAG 
GGCCGCTGCC CGGCTTCCTA TACACGCTTC ACCACCATGA TCAAGCATGA GAGGGCCAAG 
CAGGATCGGA GGCAACATTT GCGAGTAGAA GATTCTGAGT TTGGTCTCAG TAAGCTTGCA 
TTCCTGGTCA GCCCAGTGCC TTTCCGTCGG AAGAACCTGT CACCTTTGAG TTTTTCCCGA 
ATGTCAAACT CCAAGAGCTA TGTGTACGAG ACTCTAGATT CAGCTCTAAA AGACATCTAT 
GATCACATCC GCACTGAGAA ACGCAGGAGT AGCCTGTCAG AAAACAGCAT CCTTCATAGG 
TTGTTGGTGG AGTTGCTTCC GGACATTCCT GAGAGGAATT CCTCCCTGCA TGCACTGGGG 
AGAAACAGTC CTACAATTCA ACCTCGCTCT TACCACCCAC AACCTGATGG CATGTCCAGT 
CAGGACATTC ACCAATCCGA GTACTGTCAC CTGTCCCATA GTGCCTCTCA CAACCATATA 
GACAGCAACA ACAACCAGAG GAACCACAAC TTTAAATACT GCTATCAAGA CCAGGACACA 
TCCAGAAATT ACACTTACCC TGATGTGGGC CGGCAAACAC CACAGAGCAG GAGACAAACC 
CCTGATGTCA GAGAGAAACT GCCAGCCAGA GCAATATATG ACTTCAAAGC ACAGACGGCC 
AAAGAGCTGA CGTTCAAAAA AGGAGACACA GTATACATCA GTCGGCAGAT AGATAATAAC 
TGGTATGAAG GAGAATACCA TGGACATGTA GGGATATTCC CCATCTCATA TGTGGAGAAA 
ATCCCTCCAT CAGAGCGACA TCTGCCAGCA AGACCACCTC CTCCAGCCCA GAGCAAAGAA 
ATTGGAGAAG CAGTTGCCCG TTACAACTTT AATGCTGATA CTAATGTGGA ACTTTCATTA 
AGAAAAGGAG AGCGTGTTGT ATTGGTGCGG CAGGTGGACA AAAACTGGTT CGAGGGAAAG 
ATCCCAGGTA CAAACAAACA AGGAATATTT CCAGTGTCGT ATGTGGATGT CATTAAGAAG 
ACTTCAGTAC AAAATACTGG ACAACCGCCT GGGCCTAGTA TACCCACCAG CTTCTCCAGT 
GACAGACTGA ACAGTAGGTC CCAAAGACAA GCATATGCTT GTGATCCACT TCAAGGTGGA 
GGGGAACCAT TTCAAGCACT GTATAACTAC ATGCCACGGA ATGAAGATGA GCTGGAGCTG 
AAGGAAGGTG ATGTTGTTGA TGTCATGGAG AAGTGTGATG ATGGATGGTT TGTGGGAACG 
TCAAGAAGAA CCAAGTTTTT TGGAACCTTT CCGGGGAACT ATGTGAAGAG GCTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sorbs2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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