slc12a4 cDNA ORF clone, Danio rerio(zebrafish)

The following slc12a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13248 XM_686199.7
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Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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ODa13248 XM_686199.6 Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa13248
    Clone ID Related Accession (Same CDS sequence) XM_686199.6 , XM_686199.7
    Accession Version XM_686199.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product solute carrier family 12 member 4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_686199.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
    GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA
    GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT
    TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA
    GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT
    GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA
    GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC
    ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC
    CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG
    AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC
    TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC
    GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG
    GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG
    CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA
    TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC
    TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA
    AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT
    GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT
    CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG
    GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA
    GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC
    GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC
    ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA
    CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT
    TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT
    AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC
    TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA
    GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT
    GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT
    GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT
    GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC
    AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC
    ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC
    ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC
    GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG
    CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG
    CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT
    CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC
    ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA
    GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT
    GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT
    ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG
    TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT
    GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG
    CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG
    AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC
    GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG
    AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG
    CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG
    GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA
    GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA
    CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC
    AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA
    GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG
    CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_691291.2
    CDS276..3563
    Translation

    Target ORF information:

    RefSeq Version XM_686199.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_686199.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
    GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA
    GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT
    TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA
    GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT
    GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA
    GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC
    ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC
    CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG
    AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC
    TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC
    GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG
    GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG
    CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA
    TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC
    TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA
    AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT
    GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT
    CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG
    GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA
    GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC
    GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC
    ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA
    CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT
    TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT
    AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC
    TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA
    GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT
    GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT
    GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT
    GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC
    AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC
    ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC
    ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC
    GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG
    CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG
    CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT
    CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC
    ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA
    GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT
    GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT
    ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG
    TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT
    GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG
    CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG
    AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC
    GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG
    AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG
    CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG
    GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA
    GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA
    CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC
    AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA
    GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG
    CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13248
    Clone ID Related Accession (Same CDS sequence) XM_686199.6 , XM_686199.7
    Accession Version XM_686199.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product solute carrier family 12 member 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_686199.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
    GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA
    GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT
    TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA
    GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT
    GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA
    GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC
    ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC
    CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG
    AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC
    TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC
    GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG
    GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG
    CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA
    TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC
    TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA
    AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT
    GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT
    CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG
    GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA
    GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC
    GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC
    ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA
    CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT
    TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT
    AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC
    TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA
    GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT
    GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT
    GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT
    GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC
    AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC
    ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC
    ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC
    GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG
    CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG
    CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT
    CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC
    ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA
    GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT
    GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT
    ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG
    TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT
    GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG
    CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG
    AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC
    GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG
    AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG
    CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG
    GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA
    GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA
    CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC
    AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA
    GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG
    CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_691291.2
    CDS262..3549
    Translation

    Target ORF information:

    RefSeq Version XM_686199.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_686199.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTCATT TCACTGTGGT ACCAGTGCAG GATAACTCAC CGTCCAACTA TGATAGCCTG 
    GAAGGAATAA ACTGGGTTGA TTACCGGGAC ACAGGACAGG GAGAATACCC TGGACATGGA
    GACACAGTTA GCTCAGATGG TCATGGTAAT CATAAAGAAG ACAGTCCCTT CCTCAGCAGT
    TCAGACTCAG CATCAAAGAG GAATGACTTC TACGACAGAA ACCTGGCCCT GTTTGAGGAA
    GAGCTGGACA TCCGGCCGAA GGTCTCGTCT CTTCTCAGCC GCCTGGTCAA TTACACAAAT
    GTTACTCAGG GTGCCAAAGA ACATGAAGAG GCAGAAAGTG CAGATGCATC CAGACGACAA
    GCACCCAAGT CTCCAAATAT GGGCACGCTA ATGGGAGTTT ACCTGCCGTG TCTGCAGAAC
    ATCTTTGGTG TGATTCTGTT TCTGCGTCTC ACCTGGATAG TAGGAACAGC AGGTGTCGTC
    CAGTCCTTCC TCATCGTTCT CATGTGCTGT TCCTGCACAA TGTTGACAGC AATCTCGATG
    AGTGCCATAG CCACCAATGG TGTAGTTCCA GCTGGTGGGG CTTATTTCAT GATATCTCGC
    TCTCTGGGCC CTGAGTTCGG TGGTGCTGTT GGTCTCTGTT TTTATTTAGG TACAACCTTC
    GCTGCTGCCA TGTACATATT GGGAGCAATT GAAATATTTC TGAAATATCT GATTCCTCAG
    GCTGCCATAT TCCATCCAGC AGATATTCAC GCAGGAAACG GTGCAATGCT GAATAACATG
    CGGGTTTATG GCACAATATG TTTAAGCCTC ATGGCTGTGG TTGTTTTTGT GGGGGTCAAA
    TATGTCAACA AATTAGCCTC GCTGTTCCTA GCCTGCGTCA TAATCTCCAT TGTTTCTATC
    TATGCTGGAG CGGTCAAGTC CATCTTCCAC CCTCCTGAGT TTCCGATCTG CATGTTAGGA
    AACCGGACAT TGGTTCGTGA TTTGTTTGAT GTTTGTGGGA AGACGGTAGT TGTCGGTAAT
    GAAACGGTCC CCAGCAAGTT GTGGAAGAAC TTCTGCACCT CTGATAACAT CAGCAGTGCT
    CACTGTGATG AGTACTTCTT CCACAACAAT GTTACAGAGA TCAAGGGCAT TCCTGGGCTG
    GCCAGTGGCA TCATTAAAGA AAACATGTGG GGGGATTACA TGGAGAAAGG ACAGATTCTA
    GAGAAGGCAA AACTGCCCTC TGTTGATGTC CATGGTTCCA TGGAGACATT TGGCTTCTAC
    GTGTCTGCTG ACATCGCTAC ATCCTTCACC CTTTTAGTGG GGATATTTTT TCCTTCTGCC
    ACAGGAATTA TGGCAGGATC AAACAGATCT GGAGATCTAA GAGACGCACA GAAGTCTATA
    CCAATTGGGA CGATTCTGGC CATAACAACG ACCTCTCTTG TCTATTTTAG CTCTGTGGTT
    TTGTTCGGAG CCTGTATTGA GGGTGTGGTG CTCAGAGATA AATTTGGAGA TGCAGTGAGT
    AAAAACCTAG TAGTTGGCAC ACTGTCCTGG CCCTCACCCT GGGTCATTGT GATTGGCTCC
    TTCTTCTCCA CCGTGGGTGC AGGTCTTCAG TCCCTGACCG GAGCTCCTCG TCTCCTGCAA
    GCCATCGCTA AAGATAACAT CATTCCTTTC CTCAGAGTCT TTGGACACGG TAAAGCTAAT
    GGAGAACCCA CATGGGCCCT GCTGCTGACA GGACTCATAG CAGAGCTTGG CATCCTCATT
    GCATCACTGG ATATGGTCGC TCCTATTCTC TCCATGTTTT TCCTGATGTG TTATCTATTT
    GTAAACTTGG CATGTGCAGT ACAAACACTA TTAAGGACAC CAAACTGGAG ACCGCGGTTC
    AGATATTACC ATTGGGCTCT GTCTTTTCTG GGCATGAGTA TGTGTTTAGC CCTTATGTTC
    ATCTCCTCTT GGTACTATGC CATTGTAGCG ATGGGCATTG CCGGAATGAT CTACAAGTAC
    ATTGAGTATC AGGGAGCAGA GAAGGAATGG GGTGATGGGA TCCGTGGTTT GTCTCTGAGC
    GCCGCCCGCT ACTCTCTGCT TCGCCTGGAG GCTGGACCAC CCCACACCAA AAACTGGAGG
    CCTCAGCTGC TGGTTCTGTT GAAGTTGGAT GAGGATCTGC ATGTAAAGTA TCCCAGATTG
    CTTACCTTTG CCTCACAGCT GAAGGCAGGG AAAGGCCTCA CCATTGTAGG ATCTGTGATT
    CAGGGAAACT TCCTGGAATG CTATGGGGAA ACACAAGCCT CAGAACAGGC GATCAAGAAC
    ATGATGGAGA TAGAGCGAGT GAAGGGCTTC TGTCAGGTGG TGGTCGCCTC TAAAGTCAGA
    GAAGGGATTG CGCACCTCAT CCAGTCCTGT GGTCTGGGAG GCATGAAGCA CAACACTGTT
    GTTATGGGCT GGCCATATGG CTGGAGACAG AGTGAAGACC CACGGGCTTG GAAAACCTTT
    ATTAATACAG TCCGCTGTAC CACAGCTGCA CACCTAGCTT TGATGGTGCC AAAGAATGTG
    TCATTCTATC CCAGCAATCA TGAGCGTTTT ACTGATGGAT ATATTGATGT CTGGTGGATT
    GTGCATGACG GTGGAATGCT CATGCTCCTG CCTTTCCTTC TCAAACAACA CAAGGTGTGG
    CGTAAATGTA AGATGCGAAT CTTCACTGTG GCTCAAATGG ATGATAACAG TATTCAGATG
    AAGAAGGATC TGGCAACCTT CCTATACCAG CTCAGATTAG AAGCAGAGGT GGAAGTGGTC
    GAGATGCACG ACAGCGACAT CTCAGCATAT ACGTATGAGC GGACACTGAT GATGGAGCAG
    AGGTCACAGA TGCTGAAACA AATGAGGCTC TCCAGTGCAG AAAGAGACAG AGAGGCCCAG
    CTAGTGAAAG ACAGACACTC ACTGATAAGG ATGGGCAGTT TATACTCGGA TGAAGAAGAG
    GAAACCATTG AGATATTACC AGAGAAAAAC CAGATGACCT GGACTAGTGA AAAGATAGAA
    GCAGAGAGAC GTAACCGAAA CAACGCACCA GAAAACTTCA GAGAGCTAAT AAGCATTAAA
    CCTGACCAGT CTAATGTCCG GCGGATGCAC ACAGCTGTGA AGCTGAATGA GGTTATTGTC
    AACAAATCCC ATGATGCCAG ACTTGTGCTG CTAAATATGC CAGGACCTCC ACGCAATCAA
    GAGGGAGATG AAAACTATAT GGAGTTTCTC GAGGTATTAA CTGAGGGGCT AGAACGAGTG
    CTGCTTGTAA GAGGTGGAGG TCGTGAGGTC ATCACCATCT ACTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.