col2a1a cDNA ORF clone, Danio rerio(zebrafish)

The following col2a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col2a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03153 NM_131292.1
Latest version!
Danio rerio collagen, type II, alpha 1a (col2a1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa14001 XM_005166863.4
Latest version!
Danio rerio collagen, type II, alpha 1a (col2a1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
ODa14001 XM_005166863.3 Danio rerio collagen, type II, alpha 1a (col2a1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03153
    Clone ID Related Accession (Same CDS sequence) NM_131292.1
    Accession Version NM_131292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(II) chain
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ335127.1. On Jan 23, 2006 this sequence version replaced XM_687631.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ335127.1, BC168504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCAGGAAG AGTTTGGCGG CTGTGTTCAA
    GATGGCCAGC AGTATGCAGA CCGTGCGGTT TGGAAACCAG AACCGTGCCG TGTCTGCGTG
    TGTGATTCGG GGACTGTGCT GTGTGACGAG GTCATCTGTG AGGATCTGAA TGACTGTGCC
    AATCCCATCA TTTCACCTGG AGAGTGCTGT CCCATCTGCC CAGCTGACAC CGACGACCCT
    ATCGGGTCAC TTGGTGCAAA GGGACAGAAA GGAGAACCAG GCGATATTAC AGATGTTGTA
    GGACCAAGAG GACCTGCTGG ACCAATGGGA CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA
    AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC GGCCCAAGAG GGAGAGATGG CGAGCCTGGT
    ACACCTGGAA ACCCAGGACC CCCTGGACCA CCCGGACCCA ATGGTCCCCC TGGACTTGGA
    GGAAACTTTG CTGCTCAGAT GGCTGGTGGT TTTGATGAGA AGGCAGGAGG TGCTCAGATG
    GGTGTGATGC AGGGACCAAT GGGTCCAATG GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA
    GCACCTGGCC CACAAGGTTT CCAAGGCAAC CCTGGAGAGA CTGGTGAACC CGGCCCTGCT
    GGTGCCCTTG GACCCCGTGG CCCACCCGGC CCCCCTGGAA AACCCGGAAG TGATGGTGAG
    GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT GGACCCCCAG GACCTCAGGG AGCTCGTGGA
    TTCCCTGGGA CCCCTGGACT CCCTGGCATC AAGGGACACA GAGGACACCC AGGTCTGGAT
    GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT GCCAAGGGTG AGGCAGGTTC TAACGGTGAG
    AGTGGCGCTC CTGGACCAAT GGGTCCTCGT GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT
    GCAACTGGTG CTGCTGGTGC CCGTGGTAAT GATGGTCTGC CTGGTCCTGC TGGTCCTCCT
    GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT TTCCCAGGAT CCCCTGGATC TAAGGGTGAA
    GCTGGTCCCA CTGGGGCTCG TGGACCTGAG GGCGCACAGG GACCTCGTGG AGAGGCTGGT
    ACACCTGGAT CACCTGGACC CGCCGGTGCA TCCGGTAACC CTGGTACTGA TGGTATTCCT
    GGAGCTAAAG GATCTGCTGG TGCTTCTGGC ATTGCTGGTG CTCCTGGTTT CCCAGGACCA
    CGTGGTCCAC CAGGACCTCA GGGAGCCACT GGACCTCTTG GGCCTAAAGG ACAATCTGGA
    GACCCTGGTA TCCCAGGATT CAAAGGCGAG GCTGGACCCA AGGGAGAGCG TGGTGTTCTA
    GGACCTCAAG GACCTCCTGG GCCATCAGGT GAAGAGGGCA AGAGAGGACC AAGAGGAGAG
    CCTGGTTCTG CCGGACCTCT TGGACCTCCT GGAGAGAGAG GAGCTCCAGG TAACCGTGGA
    TTCCCTGGCC AGGATGGACT TGCAGGAGCA AAGGGTGCTC CTGGTGATCG TGGTGTTCCA
    GGTTTGAGTG GACCGAAAGG AGGCACTGGA GATCCTGGTC GCCCTGGTGA ACCTGGTCTT
    CCTGGAGCCA GAGGTCTCAC TGGACGCCCT GGTGATGCTG GAGCTCAAGG AAAAGTTGGA
    GCGACTGGTG CCCCTGGTGA AGATGGCCGC CCTGGACCAC CTGGGCCTCT GGGAGCTCGT
    GGTCAGCCTG GAGTGATGGG ATTCCCTGGA CCCAAGGGAG CCAATGGTGA ACCTGGAAAA
    CCTGGAGAGA AAGGACTTGT TGGACGCACT GGTCTTAGAG GTTTGCCTGG TAAAGATGGA
    GAGACTGGAC CTTCTGGTCC TCCTGGCCCT GTTGGTGCTG TTGGTGAGAG AGGAGAACAA
    GGTCAACCTG GTCCATCTGG CTTCCAGGGT CTGCCTGGAC CTACTGGAGC CCCCGGAGAA
    CCTGGTAAAC CTGGTGACCA GGGTGTTCCT GGAGAGGGTG GTGCTGCCGG TCCCACTGGA
    CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA
    GGACCAAGAG GACTTCCAGG AACTCCCGGA ACAGATGGAC CCAAGGGTGC GATTGGGCCA
    GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC GGTCTTCAAG GCATGCCTGG TGAGAGAGGA
    GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC AGAGGTGACT CTGGAGAGAA GGGACCTGAA
    GGTGCTCCTG GTAAAGATGG TTCAAGAGGT TTGACTGGTC CAATTGGCCC ACCTGGTCCT
    TCTGGTCCCA ATGGCGCAAA GGGTGAAACT GGTCCTATTG GTTCAATTGG TGCTCCTGGT
    GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG ATTGGTGCAC CTGGACCTGC TGGCTTTGCT
    GGACCCCCTG GTGCTGATGG CCAACCTGGA AATAAGGGAG AGCAGGGTGA ATCAGGACAG
    AAAGGTGATT CAGGAGCCCC TGGACCCCAA GGACCATCAG GTGCTCCAGG CCCAGTGGGC
    CCAACTGGTG TCACAGGACC TAAGGGAGCA CGTGGAGCCC AAGGAGCCCC TGGTGCCACT
    GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA CCCCCTGGCC CCAACGGTAA CCCTGGTGCT
    GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT GGTCCTAAGG GAGTTCGTGG TGATGCCGGT
    CCCCCAGGTA GAGCAGGAGA TGCTGGACTG CGTGGACCTC CTGGTGCTCC TGGAGAGAAG
    GGAGAGGCTG GTGAAGATGG TCCTCCTGGT CCTGATGGTC CTTCCGGCCC TGCTGGTCTT
    GCTGGTCAAC GTGGTATTGT TGGTTTGCCT GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA
    CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA CAGGGAGCTC CAGGAGGCTC TGGTGACCGT
    GGACCTCCTG GCCCTGTTGG ACCACCTGGA TTGACTGGAC CTGCTGGAGA GACTGGCCGT
    GAGGGTAATC CTGGATCTGA TGGTCCACCT GGTAGAGATG GTGCTGCTGG TGTGAAGGGT
    GAGCGTGGTA ACACTGGCCC AATTGGTGCT CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT
    GGTTCAGTTG GTCCAATTGG CAAACAGGGA GACAGAGGAG AGAATGGCCC ACAAGGACCT
    GCTGGACCCC CTGGACCCGC TGGAGCTCGA GGCATGGTTG GACCCCAAGG ACCTCGTGGT
    GATAAGGGTG AGGCAGGAGA AGCTGGAGAA AGAGGCCAGA AGGGACACAG AGGATTCACT
    GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT TCTCCTGGAG ACCAGGGTGC TGCTGGACCT
    GCTGGACCTT CTGGTGCTAA AGGACCTTCA GGACCTGTTG GACCTGCTGG TAAGGATGGT
    TCTAATGGTC AGCCTGGACC CATTGGTCCA CCTGGACCTC GTGGACGCTC TGGAGAGTCT
    GGCCCAGTTG GTCCTCCTGG TAACCCCGGA CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT
    GGCATTGACA TGTCTGCCTT CGCTGGATTG TCTCAGCCAG AGAAGGGACC CGATCCTCTG
    CGTTACATGC GTGCAGATGA AGCCTCCAGC TCTCTCAGAC AACACGATGT AGAGGTGGAC
    GCCACTCTGA AGTCCATCAA CGGGCAGATT GAGGATATCC GCAGCCCCGA TGGCTCCCGC
    AAGAATCCTG CACGCTCCTG CCGAGACCTG AAACTCTGCC ACCCTGAATG GAAGAGCGGT
    GACTACTGGG TGGATCCCAA CCTTGGTAGC GCTGCTGATG CCATCAAAGT TTTCTGCAAC
    ATGGAGACCG GAGAGACCTG TGTGAAACCA AGCACTCCTA AAATCCCACG CAAGAACTGG
    TGGACAAGCA AGAGCAAGGC CCAGAAGCAC GTGTGGTTCG GAGAAAGCAT GAACGGCGGA
    TTCCACTTTA GCTATGCTGA TGGCAGCCAG ACTCCTAGTA CTACCACCAT CCAGCTGAAC
    TTCCTCAGGC TGCTGTCTAC TGAGGCCACT CAGACCATCA CCTACCACTG CAAGAACAGC
    GTGGCTTACA TGGACCAAGC CACAGGCAAC CTGAAGAAGG CCATTCTGCT GCAGGGCTCC
    AATGATGTGG AGATCAGAGC AGAGGGCAAC AGCCGCTTTA CATATGGCGT ACTTGAGGAT
    GGCTGCAAGA AGCACACAGG TCAATGGGCC AAGACTGTGA TCGAGTACAA AACTCAGAAG
    ACATCCAGAC TGCCCATCAT GGACATCGCT CCAATGGACA TCGGAGGAGC AGATCAGGAG
    TTCGGAGTGG ACATTGGCGC AGTCTGCTTC TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571367.1
    CDS45..4520
    Translation

    Target ORF information:

    RefSeq Version NM_131292.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type II, alpha 1a (col2a1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCAGGAAG AGTTTGGCGG CTGTGTTCAA
    GATGGCCAGC AGTATGCAGA CCGTGCGGTT TGGAAACCAG AACCGTGCCG TGTCTGCGTG
    TGTGATTCGG GGACTGTGCT GTGTGACGAG GTCATCTGTG AGGATCTGAA TGACTGTGCC
    AATCCCATCA TTTCACCTGG AGAGTGCTGT CCCATCTGCC CAGCTGACAC CGACGACCCT
    ATCGGGTCAC TTGGTGCAAA GGGACAGAAA GGAGAACCAG GCGATATTAC AGATGTTGTA
    GGACCAAGAG GACCTGCTGG ACCAATGGGA CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA
    AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC GGCCCAAGAG GGAGAGATGG CGAGCCTGGT
    ACACCTGGAA ACCCAGGACC CCCTGGACCA CCCGGACCCA ATGGTCCCCC TGGACTTGGA
    GGAAACTTTG CTGCTCAGAT GGCTGGTGGT TTTGATGAGA AGGCAGGAGG TGCTCAGATG
    GGTGTGATGC AGGGACCAAT GGGTCCAATG GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA
    GCACCTGGCC CACAAGGTTT CCAAGGCAAC CCTGGAGAGA CTGGTGAACC CGGCCCTGCT
    GGTGCCCTTG GACCCCGTGG CCCACCCGGC CCCCCTGGAA AACCCGGAAG TGATGGTGAG
    GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT GGACCCCCAG GACCTCAGGG AGCTCGTGGA
    TTCCCTGGGA CCCCTGGACT CCCTGGCATC AAGGGACACA GAGGACACCC AGGTCTGGAT
    GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT GCCAAGGGTG AGGCAGGTTC TAACGGTGAG
    AGTGGCGCTC CTGGACCAAT GGGTCCTCGT GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT
    GCAACTGGTG CTGCTGGTGC CCGTGGTAAT GATGGTCTGC CTGGTCCTGC TGGTCCTCCT
    GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT TTCCCAGGAT CCCCTGGATC TAAGGGTGAA
    GCTGGTCCCA CTGGGGCTCG TGGACCTGAG GGCGCACAGG GACCTCGTGG AGAGGCTGGT
    ACACCTGGAT CACCTGGACC CGCCGGTGCA TCCGGTAACC CTGGTACTGA TGGTATTCCT
    GGAGCTAAAG GATCTGCTGG TGCTTCTGGC ATTGCTGGTG CTCCTGGTTT CCCAGGACCA
    CGTGGTCCAC CAGGACCTCA GGGAGCCACT GGACCTCTTG GGCCTAAAGG ACAATCTGGA
    GACCCTGGTA TCCCAGGATT CAAAGGCGAG GCTGGACCCA AGGGAGAGCG TGGTGTTCTA
    GGACCTCAAG GACCTCCTGG GCCATCAGGT GAAGAGGGCA AGAGAGGACC AAGAGGAGAG
    CCTGGTTCTG CCGGACCTCT TGGACCTCCT GGAGAGAGAG GAGCTCCAGG TAACCGTGGA
    TTCCCTGGCC AGGATGGACT TGCAGGAGCA AAGGGTGCTC CTGGTGATCG TGGTGTTCCA
    GGTTTGAGTG GACCGAAAGG AGGCACTGGA GATCCTGGTC GCCCTGGTGA ACCTGGTCTT
    CCTGGAGCCA GAGGTCTCAC TGGACGCCCT GGTGATGCTG GAGCTCAAGG AAAAGTTGGA
    GCGACTGGTG CCCCTGGTGA AGATGGCCGC CCTGGACCAC CTGGGCCTCT GGGAGCTCGT
    GGTCAGCCTG GAGTGATGGG ATTCCCTGGA CCCAAGGGAG CCAATGGTGA ACCTGGAAAA
    CCTGGAGAGA AAGGACTTGT TGGACGCACT GGTCTTAGAG GTTTGCCTGG TAAAGATGGA
    GAGACTGGAC CTTCTGGTCC TCCTGGCCCT GTTGGTGCTG TTGGTGAGAG AGGAGAACAA
    GGTCAACCTG GTCCATCTGG CTTCCAGGGT CTGCCTGGAC CTACTGGAGC CCCCGGAGAA
    CCTGGTAAAC CTGGTGACCA GGGTGTTCCT GGAGAGGGTG GTGCTGCCGG TCCCACTGGA
    CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA
    GGACCAAGAG GACTTCCAGG AACTCCCGGA ACAGATGGAC CCAAGGGTGC GATTGGGCCA
    GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC GGTCTTCAAG GCATGCCTGG TGAGAGAGGA
    GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC AGAGGTGACT CTGGAGAGAA GGGACCTGAA
    GGTGCTCCTG GTAAAGATGG TTCAAGAGGT TTGACTGGTC CAATTGGCCC ACCTGGTCCT
    TCTGGTCCCA ATGGCGCAAA GGGTGAAACT GGTCCTATTG GTTCAATTGG TGCTCCTGGT
    GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG ATTGGTGCAC CTGGACCTGC TGGCTTTGCT
    GGACCCCCTG GTGCTGATGG CCAACCTGGA AATAAGGGAG AGCAGGGTGA ATCAGGACAG
    AAAGGTGATT CAGGAGCCCC TGGACCCCAA GGACCATCAG GTGCTCCAGG CCCAGTGGGC
    CCAACTGGTG TCACAGGACC TAAGGGAGCA CGTGGAGCCC AAGGAGCCCC TGGTGCCACT
    GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA CCCCCTGGCC CCAACGGTAA CCCTGGTGCT
    GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT GGTCCTAAGG GAGTTCGTGG TGATGCCGGT
    CCCCCAGGTA GAGCAGGAGA TGCTGGACTG CGTGGACCTC CTGGTGCTCC TGGAGAGAAG
    GGAGAGGCTG GTGAAGATGG TCCTCCTGGT CCTGATGGTC CTTCCGGCCC TGCTGGTCTT
    GCTGGTCAAC GTGGTATTGT TGGTTTGCCT GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA
    CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA CAGGGAGCTC CAGGAGGCTC TGGTGACCGT
    GGACCTCCTG GCCCTGTTGG ACCACCTGGA TTGACTGGAC CTGCTGGAGA GACTGGCCGT
    GAGGGTAATC CTGGATCTGA TGGTCCACCT GGTAGAGATG GTGCTGCTGG TGTGAAGGGT
    GAGCGTGGTA ACACTGGCCC AATTGGTGCT CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT
    GGTTCAGTTG GTCCAATTGG CAAACAGGGA GACAGAGGAG AGAATGGCCC ACAAGGACCT
    GCTGGACCCC CTGGACCCGC TGGAGCTCGA GGCATGGTTG GACCCCAAGG ACCTCGTGGT
    GATAAGGGTG AGGCAGGAGA AGCTGGAGAA AGAGGCCAGA AGGGACACAG AGGATTCACT
    GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT TCTCCTGGAG ACCAGGGTGC TGCTGGACCT
    GCTGGACCTT CTGGTGCTAA AGGACCTTCA GGACCTGTTG GACCTGCTGG TAAGGATGGT
    TCTAATGGTC AGCCTGGACC CATTGGTCCA CCTGGACCTC GTGGACGCTC TGGAGAGTCT
    GGCCCAGTTG GTCCTCCTGG TAACCCCGGA CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT
    GGCATTGACA TGTCTGCCTT CGCTGGATTG TCTCAGCCAG AGAAGGGACC CGATCCTCTG
    CGTTACATGC GTGCAGATGA AGCCTCCAGC TCTCTCAGAC AACACGATGT AGAGGTGGAC
    GCCACTCTGA AGTCCATCAA CGGGCAGATT GAGGATATCC GCAGCCCCGA TGGCTCCCGC
    AAGAATCCTG CACGCTCCTG CCGAGACCTG AAACTCTGCC ACCCTGAATG GAAGAGCGGT
    GACTACTGGG TGGATCCCAA CCTTGGTAGC GCTGCTGATG CCATCAAAGT TTTCTGCAAC
    ATGGAGACCG GAGAGACCTG TGTGAAACCA AGCACTCCTA AAATCCCACG CAAGAACTGG
    TGGACAAGCA AGAGCAAGGC CCAGAAGCAC GTGTGGTTCG GAGAAAGCAT GAACGGCGGA
    TTCCACTTTA GCTATGCTGA TGGCAGCCAG ACTCCTAGTA CTACCACCAT CCAGCTGAAC
    TTCCTCAGGC TGCTGTCTAC TGAGGCCACT CAGACCATCA CCTACCACTG CAAGAACAGC
    GTGGCTTACA TGGACCAAGC CACAGGCAAC CTGAAGAAGG CCATTCTGCT GCAGGGCTCC
    AATGATGTGG AGATCAGAGC AGAGGGCAAC AGCCGCTTTA CATATGGCGT ACTTGAGGAT
    GGCTGCAAGA AGCACACAGG TCAATGGGCC AAGACTGTGA TCGAGTACAA AACTCAGAAG
    ACATCCAGAC TGCCCATCAT GGACATCGCT CCAATGGACA TCGGAGGAGC AGATCAGGAG
    TTCGGAGTGG ACATTGGCGC AGTCTGCTTC TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14001
    Clone ID Related Accession (Same CDS sequence) XM_005166863.3 , XM_005166863.4
    Accession Version XM_005166863.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product collagen alpha-1(II) chain isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCGGTCAC TTGGTGCAAA GGGACAGAAA
    GGAGAACCAG GCGATATTAC AGATGTTGTA GGACCAAGAG GACCTGCTGG ACCAATGGGA
    CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC
    GGCCCAAGAG GGAGAGATGG CGAGCCTGGT ACACCTGGAA ACCCAGGACC CCCTGGACCA
    CCCGGACCCA ATGGTCCCCC TGGACTTGGA GGAAACTTTG CTGCTCAGAT GGCTGGTGGT
    TTTGATGAGA AGGCAGGAGG TGCTCAGATG GGTGTGATGC AGGGACCAAT GGGTCCAATG
    GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA GCACCTGGCC CACAAGGTTT CCAAGGCAAC
    CCTGGAGAGA CTGGTGAACC CGGCCCTGCT GGTGCCCTTG GACCCCGTGG CCCACCCGGC
    CCCCCTGGAA AACCCGGAAG TGATGGTGAG GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT
    GGACCCCCAG GACCTCAGGG AGCTCGTGGA TTCCCTGGGA CCCCTGGACT CCCTGGCATC
    AAGGGACACA GAGGACACCC AGGTCTGGAT GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT
    GCCAAGGGTG AGGCAGGTTC TAACGGTGAG AGTGGCGCTC CTGGACCAAT GGGTCCTCGT
    GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT GCAACTGGTG CTGCTGGTGC CCGTGGTAAT
    GATGGTCTGC CTGGTCCTGC TGGTCCTCCT GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT
    TTCCCAGGAT CCCCTGGATC TAAGGGTGAA GCTGGTCCCA CTGGGGCTCG TGGACCTGAG
    GGCGCACAGG GACCTCGTGG AGAGGCTGGT ACACCTGGAT CACCTGGACC CGCCGGTGCA
    TCCGGTAACC CTGGTACTGA TGGTATTCCT GGAGCTAAAG GATCTGCTGG TGCTTCTGGC
    ATTGCTGGTG CTCCTGGTTT CCCAGGACCA CGTGGTCCAC CAGGACCTCA GGGAGCCACT
    GGACCTCTTG GGCCTAAAGG ACAATCTGGA GACCCTGGTA TCCCAGGATT CAAAGGCGAG
    GCTGGACCCA AGGGAGAGCG TGGTGTTCTA GGACCTCAAG GACCTCCTGG GCCATCAGGT
    GAAGAGGGCA AGAGAGGACC AAGAGGAGAG CCTGGTTCTG CCGGACCTCT TGGACCTCCT
    GGAGAGAGAG GAGCTCCAGG TAACCGTGGA TTCCCTGGCC AGGATGGACT TGCAGGAGCA
    AAGGGTGCTC CTGGTGATCG TGGTGTTCCA GGTTTGAGTG GACCGAAAGG AGGCACTGGA
    GATCCTGGTC GCCCTGGTGA ACCTGGTCTT CCTGGAGCCA GAGGTCTCAC TGGACGCCCT
    GGTGATGCTG GAGCTCAAGG AAAAGTCGGA GCGACTGGTG CCCCTGGTGA AGATGGCCGC
    CCTGGACCAC CTGGGCCTCT GGGAGCTCGT GGTCAGCCTG GAGTGATGGG ATTCCCTGGA
    CCCAAGGGAG CCAATGGTGA ACCTGGAAAA CCTGGAGAGA AAGGACTTGT TGGACGCACT
    GGTCTTAGAG GTTTGCCTGG TAAAGATGGA GAGACTGGAC CTTCTGGTCC TCCTGGCCCT
    GTTGGTGCTG TTGGTGAGAG AGGAGAACAA GGTCAACCTG GTCCATCTGG CTTCCAGGGT
    CTGCCTGGAC CTACTGGAGC CCCCGGAGAA CCTGGTAAAC CTGGTGACCA GGGTGTTCCT
    GGAGAGGGTG GTGCTGCCGG TCCCACTGGA CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG
    AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA GGACCAAGAG GACTTCCAGG AACTCCCGGA
    ACAGATGGAC CCAAGGGTGC GATTGGGCCA GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC
    GGTCTTCAAG GCATGCCTGG TGAGAGAGGA GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC
    AGAGGTGACT CTGGAGAGAA GGGACCTGAA GGTGCTCCTG GTAAAGATGG TTCAAGAGGT
    TTGACTGGTC CAATTGGCCC ACCTGGTCCT TCTGGTCCCA ATGGCGCAAA GGGTGAAACT
    GGTCCTATTG GTTCAATTGG TGCTCCTGGT GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG
    ATTGGTGCAC CTGGACCTGC TGGCTTTGCT GGACCCCCTG GTGCTGATGG CCAACCTGGA
    AATAAGGGAG AGCAGGGTGA ATCAGGACAG AAAGGTGATT CAGGAGCCCC TGGACCCCAA
    GGACCATCAG GTGCTCCAGG CCCAGTGGGC CCAACTGGTG TCACAGGACC TAAGGGAGCA
    CGTGGAGCCC AAGGAGCCCC TGGTGCCACT GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA
    CCCCCTGGCC CCAACGGTAA CCCTGGTGCT GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT
    GGTCCTAAGG GAGTTCGTGG TGATGCCGGT CCCCCAGGTA GAGCAGGAGA TGCTGGACTG
    CGTGGACCTC CTGGTGCTCC TGGAGAGAAG GGAGAGGCTG GTGAAGATGG TCCTCCTGGT
    CCTGATGGTC CTTCCGGCCC TGCTGGTCTT GCTGGTCAGC GTGGTATTGT TGGTTTGCCT
    GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA
    CAGGGAGCTC CAGGAGGCTC TGGTGACCGT GGACCTCCTG GCCCTGTTGG CCCACCTGGA
    TTGACTGGAC CTGCTGGAGA GACTGGCCGT GAGGGTAATC CTGGATCTGA TGGTCCACCT
    GGTAGAGATG GTGCTGCTGG TGTGAAGGGT GAGCGTGGTA ACACTGGCCC AATTGGTGCT
    CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT GGTTCAGTTG GTCCAATTGG CAAACAGGGA
    GACAGAGGAG AGAATGGCCC ACAAGGACCT GCTGGACCCC CTGGACCCGC TGGAGCTCGA
    GGCATGGTTG GACCCCAAGG ACCTCGTGGT GATAAGGGTG AGGCAGGAGA AGCTGGAGAA
    AGAGGCCAGA AGGGACACAG AGGATTCACT GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT
    TCTCCTGGAG ACCAGGGTGC TGCTGGACCT GCTGGACCTT CTGGTGCTAA AGGACCTTCA
    GGACCTGTTG GACCTGCTGG TAAGGATGGT TCTAATGGTC AGCCTGGACC CATTGGTCCA
    CCTGGACCTC GTGGACGCTC TGGAGAGTCT GGCCCAGTTG GTCCTCCTGG TAACCCCGGA
    CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT GGCATTGACA TGTCTGCCTT CGCTGGATTG
    TCTCAGCCAG AGAAGGGACC CGATCCTCTG CGTTACATGC GTGCAGATGA AGCCTCCAGC
    TCTCTCAGAC AACACGATGT GGAGGTGGAC GCCACTCTGA AGTCCATCAA CGGGCAGATT
    GAGGATATCC GCAGCCCCGA TGGCTCCCGC AAGAATCCTG CACGCTCCTG CCGAGACCTG
    AAACTCTGCC ACCCTGAATG GAAGAGCGGT GACTACTGGG TGGATCCCAA CCTTGGTAGC
    GCTGCTGATG CCATCAAAGT TTTCTGCAAC ATGGAGACCG GAGAGACCTG TGTGAAACCA
    AGCACTCCTA AAATCCCACG CAAGAACTGG TGGACAAGCA AGAGCAAGGC CCAGAAGCAC
    GTGTGGTTCG GAGAAAGCAT GAACGGCGGA TTCCACTTTA GCTATGCTGA TGGCAGCCAG
    ACTCCTAGTA CTACCACCAT CCAGCTGAAC TTCCTCAGGC TGCTGTCTAC TGAGGCCACT
    CAGACCATCA CCTACCACTG CAAGAACAGC GTGGCTTACA TGGACCAAGC CACAGGCAAC
    CTGAAGAAGG CCATTCTGCT GCAGGGCTCC AATGATGTGG AGATCAGAGC AGAGGGCAAC
    AGCCGCTTTA CATATGGCGT ACTTGAGGAT GGCTGCAAGA AGCACACAGG TCAATGGGCC
    AAGACTGTGA TCGAGTACAA AACTCAGAAG ACATCCAGAC TGCCCATCAT GGACATCGCT
    CCAATGGACA TCGGAGGCGC AGATCAGGAG TTCGGAGTGG ACATTGGCGC AGTCTGCTTC
    TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166920.1
    CDS199..4464
    Translation

    Target ORF information:

    RefSeq Version XM_005166863.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio collagen, type II, alpha 1a (col2a1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166863.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCGGTCAC TTGGTGCAAA GGGACAGAAA
    GGAGAACCAG GCGATATTAC AGATGTTGTA GGACCAAGAG GACCTGCTGG ACCAATGGGA
    CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC
    GGCCCAAGAG GGAGAGATGG CGAGCCTGGT ACACCTGGAA ACCCAGGACC CCCTGGACCA
    CCCGGACCCA ATGGTCCCCC TGGACTTGGA GGAAACTTTG CTGCTCAGAT GGCTGGTGGT
    TTTGATGAGA AGGCAGGAGG TGCTCAGATG GGTGTGATGC AGGGACCAAT GGGTCCAATG
    GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA GCACCTGGCC CACAAGGTTT CCAAGGCAAC
    CCTGGAGAGA CTGGTGAACC CGGCCCTGCT GGTGCCCTTG GACCCCGTGG CCCACCCGGC
    CCCCCTGGAA AACCCGGAAG TGATGGTGAG GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT
    GGACCCCCAG GACCTCAGGG AGCTCGTGGA TTCCCTGGGA CCCCTGGACT CCCTGGCATC
    AAGGGACACA GAGGACACCC AGGTCTGGAT GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT
    GCCAAGGGTG AGGCAGGTTC TAACGGTGAG AGTGGCGCTC CTGGACCAAT GGGTCCTCGT
    GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT GCAACTGGTG CTGCTGGTGC CCGTGGTAAT
    GATGGTCTGC CTGGTCCTGC TGGTCCTCCT GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT
    TTCCCAGGAT CCCCTGGATC TAAGGGTGAA GCTGGTCCCA CTGGGGCTCG TGGACCTGAG
    GGCGCACAGG GACCTCGTGG AGAGGCTGGT ACACCTGGAT CACCTGGACC CGCCGGTGCA
    TCCGGTAACC CTGGTACTGA TGGTATTCCT GGAGCTAAAG GATCTGCTGG TGCTTCTGGC
    ATTGCTGGTG CTCCTGGTTT CCCAGGACCA CGTGGTCCAC CAGGACCTCA GGGAGCCACT
    GGACCTCTTG GGCCTAAAGG ACAATCTGGA GACCCTGGTA TCCCAGGATT CAAAGGCGAG
    GCTGGACCCA AGGGAGAGCG TGGTGTTCTA GGACCTCAAG GACCTCCTGG GCCATCAGGT
    GAAGAGGGCA AGAGAGGACC AAGAGGAGAG CCTGGTTCTG CCGGACCTCT TGGACCTCCT
    GGAGAGAGAG GAGCTCCAGG TAACCGTGGA TTCCCTGGCC AGGATGGACT TGCAGGAGCA
    AAGGGTGCTC CTGGTGATCG TGGTGTTCCA GGTTTGAGTG GACCGAAAGG AGGCACTGGA
    GATCCTGGTC GCCCTGGTGA ACCTGGTCTT CCTGGAGCCA GAGGTCTCAC TGGACGCCCT
    GGTGATGCTG GAGCTCAAGG AAAAGTCGGA GCGACTGGTG CCCCTGGTGA AGATGGCCGC
    CCTGGACCAC CTGGGCCTCT GGGAGCTCGT GGTCAGCCTG GAGTGATGGG ATTCCCTGGA
    CCCAAGGGAG CCAATGGTGA ACCTGGAAAA CCTGGAGAGA AAGGACTTGT TGGACGCACT
    GGTCTTAGAG GTTTGCCTGG TAAAGATGGA GAGACTGGAC CTTCTGGTCC TCCTGGCCCT
    GTTGGTGCTG TTGGTGAGAG AGGAGAACAA GGTCAACCTG GTCCATCTGG CTTCCAGGGT
    CTGCCTGGAC CTACTGGAGC CCCCGGAGAA CCTGGTAAAC CTGGTGACCA GGGTGTTCCT
    GGAGAGGGTG GTGCTGCCGG TCCCACTGGA CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG
    AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA GGACCAAGAG GACTTCCAGG AACTCCCGGA
    ACAGATGGAC CCAAGGGTGC GATTGGGCCA GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC
    GGTCTTCAAG GCATGCCTGG TGAGAGAGGA GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC
    AGAGGTGACT CTGGAGAGAA GGGACCTGAA GGTGCTCCTG GTAAAGATGG TTCAAGAGGT
    TTGACTGGTC CAATTGGCCC ACCTGGTCCT TCTGGTCCCA ATGGCGCAAA GGGTGAAACT
    GGTCCTATTG GTTCAATTGG TGCTCCTGGT GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG
    ATTGGTGCAC CTGGACCTGC TGGCTTTGCT GGACCCCCTG GTGCTGATGG CCAACCTGGA
    AATAAGGGAG AGCAGGGTGA ATCAGGACAG AAAGGTGATT CAGGAGCCCC TGGACCCCAA
    GGACCATCAG GTGCTCCAGG CCCAGTGGGC CCAACTGGTG TCACAGGACC TAAGGGAGCA
    CGTGGAGCCC AAGGAGCCCC TGGTGCCACT GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA
    CCCCCTGGCC CCAACGGTAA CCCTGGTGCT GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT
    GGTCCTAAGG GAGTTCGTGG TGATGCCGGT CCCCCAGGTA GAGCAGGAGA TGCTGGACTG
    CGTGGACCTC CTGGTGCTCC TGGAGAGAAG GGAGAGGCTG GTGAAGATGG TCCTCCTGGT
    CCTGATGGTC CTTCCGGCCC TGCTGGTCTT GCTGGTCAGC GTGGTATTGT TGGTTTGCCT
    GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA
    CAGGGAGCTC CAGGAGGCTC TGGTGACCGT GGACCTCCTG GCCCTGTTGG CCCACCTGGA
    TTGACTGGAC CTGCTGGAGA GACTGGCCGT GAGGGTAATC CTGGATCTGA TGGTCCACCT
    GGTAGAGATG GTGCTGCTGG TGTGAAGGGT GAGCGTGGTA ACACTGGCCC AATTGGTGCT
    CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT GGTTCAGTTG GTCCAATTGG CAAACAGGGA
    GACAGAGGAG AGAATGGCCC ACAAGGACCT GCTGGACCCC CTGGACCCGC TGGAGCTCGA
    GGCATGGTTG GACCCCAAGG ACCTCGTGGT GATAAGGGTG AGGCAGGAGA AGCTGGAGAA
    AGAGGCCAGA AGGGACACAG AGGATTCACT GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT
    TCTCCTGGAG ACCAGGGTGC TGCTGGACCT GCTGGACCTT CTGGTGCTAA AGGACCTTCA
    GGACCTGTTG GACCTGCTGG TAAGGATGGT TCTAATGGTC AGCCTGGACC CATTGGTCCA
    CCTGGACCTC GTGGACGCTC TGGAGAGTCT GGCCCAGTTG GTCCTCCTGG TAACCCCGGA
    CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT GGCATTGACA TGTCTGCCTT CGCTGGATTG
    TCTCAGCCAG AGAAGGGACC CGATCCTCTG CGTTACATGC GTGCAGATGA AGCCTCCAGC
    TCTCTCAGAC AACACGATGT GGAGGTGGAC GCCACTCTGA AGTCCATCAA CGGGCAGATT
    GAGGATATCC GCAGCCCCGA TGGCTCCCGC AAGAATCCTG CACGCTCCTG CCGAGACCTG
    AAACTCTGCC ACCCTGAATG GAAGAGCGGT GACTACTGGG TGGATCCCAA CCTTGGTAGC
    GCTGCTGATG CCATCAAAGT TTTCTGCAAC ATGGAGACCG GAGAGACCTG TGTGAAACCA
    AGCACTCCTA AAATCCCACG CAAGAACTGG TGGACAAGCA AGAGCAAGGC CCAGAAGCAC
    GTGTGGTTCG GAGAAAGCAT GAACGGCGGA TTCCACTTTA GCTATGCTGA TGGCAGCCAG
    ACTCCTAGTA CTACCACCAT CCAGCTGAAC TTCCTCAGGC TGCTGTCTAC TGAGGCCACT
    CAGACCATCA CCTACCACTG CAAGAACAGC GTGGCTTACA TGGACCAAGC CACAGGCAAC
    CTGAAGAAGG CCATTCTGCT GCAGGGCTCC AATGATGTGG AGATCAGAGC AGAGGGCAAC
    AGCCGCTTTA CATATGGCGT ACTTGAGGAT GGCTGCAAGA AGCACACAGG TCAATGGGCC
    AAGACTGTGA TCGAGTACAA AACTCAGAAG ACATCCAGAC TGCCCATCAT GGACATCGCT
    CCAATGGACA TCGGAGGCGC AGATCAGGAG TTCGGAGTGG ACATTGGCGC AGTCTGCTTC
    TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa14001
    Clone ID Related Accession (Same CDS sequence) XM_005166863.3 , XM_005166863.4
    Accession Version XM_005166863.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen alpha-1(II) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCGGTCAC TTGGTGCAAA GGGACAGAAA
    GGAGAACCAG GCGATATTAC AGATGTTGTA GGACCAAGAG GACCTGCTGG ACCAATGGGA
    CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC
    GGCCCAAGAG GGAGAGATGG CGAGCCTGGT ACACCTGGAA ACCCAGGACC CCCTGGACCA
    CCCGGACCCA ATGGTCCCCC TGGACTTGGA GGAAACTTTG CTGCTCAGAT GGCTGGTGGT
    TTTGATGAGA AGGCAGGAGG TGCTCAGATG GGTGTGATGC AGGGACCAAT GGGTCCAATG
    GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA GCACCTGGCC CACAAGGTTT CCAAGGCAAC
    CCTGGAGAGA CTGGTGAACC CGGCCCTGCT GGTGCCCTTG GACCCCGTGG CCCACCCGGC
    CCCCCTGGAA AACCCGGAAG TGATGGTGAG GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT
    GGACCCCCAG GACCTCAGGG AGCTCGTGGA TTCCCTGGGA CCCCTGGACT CCCTGGCATC
    AAGGGACACA GAGGACACCC AGGTCTGGAT GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT
    GCCAAGGGTG AGGCAGGTTC TAACGGTGAG AGTGGCGCTC CTGGACCAAT GGGTCCTCGT
    GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT GCAACTGGTG CTGCTGGTGC CCGTGGTAAT
    GATGGTCTGC CTGGTCCTGC TGGTCCTCCT GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT
    TTCCCAGGAT CCCCTGGATC TAAGGGTGAA GCTGGTCCCA CTGGGGCTCG TGGACCTGAG
    GGCGCACAGG GACCTCGTGG AGAGGCTGGT ACACCTGGAT CACCTGGACC CGCCGGTGCA
    TCCGGTAACC CTGGTACTGA TGGTATTCCT GGAGCTAAAG GATCTGCTGG TGCTTCTGGC
    ATTGCTGGTG CTCCTGGTTT CCCAGGACCA CGTGGTCCAC CAGGACCTCA GGGAGCCACT
    GGACCTCTTG GGCCTAAAGG ACAATCTGGA GACCCTGGTA TCCCAGGATT CAAAGGCGAG
    GCTGGACCCA AGGGAGAGCG TGGTGTTCTA GGACCTCAAG GACCTCCTGG GCCATCAGGT
    GAAGAGGGCA AGAGAGGACC AAGAGGAGAG CCTGGTTCTG CCGGACCTCT TGGACCTCCT
    GGAGAGAGAG GAGCTCCAGG TAACCGTGGA TTCCCTGGCC AGGATGGACT TGCAGGAGCA
    AAGGGTGCTC CTGGTGATCG TGGTGTTCCA GGTTTGAGTG GACCGAAAGG AGGCACTGGA
    GATCCTGGTC GCCCTGGTGA ACCTGGTCTT CCTGGAGCCA GAGGTCTCAC TGGACGCCCT
    GGTGATGCTG GAGCTCAAGG AAAAGTCGGA GCGACTGGTG CCCCTGGTGA AGATGGCCGC
    CCTGGACCAC CTGGGCCTCT GGGAGCTCGT GGTCAGCCTG GAGTGATGGG ATTCCCTGGA
    CCCAAGGGAG CCAATGGTGA ACCTGGAAAA CCTGGAGAGA AAGGACTTGT TGGACGCACT
    GGTCTTAGAG GTTTGCCTGG TAAAGATGGA GAGACTGGAC CTTCTGGTCC TCCTGGCCCT
    GTTGGTGCTG TTGGTGAGAG AGGAGAACAA GGTCAACCTG GTCCATCTGG CTTCCAGGGT
    CTGCCTGGAC CTACTGGAGC CCCCGGAGAA CCTGGTAAAC CTGGTGACCA GGGTGTTCCT
    GGAGAGGGTG GTGCTGCCGG TCCCACTGGA CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG
    AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA GGACCAAGAG GACTTCCAGG AACTCCCGGA
    ACAGATGGAC CCAAGGGTGC GATTGGGCCA GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC
    GGTCTTCAAG GCATGCCTGG TGAGAGAGGA GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC
    AGAGGTGACT CTGGAGAGAA GGGACCTGAA GGTGCTCCTG GTAAAGATGG TTCAAGAGGT
    TTGACTGGTC CAATTGGCCC ACCTGGTCCT TCTGGTCCCA ATGGCGCAAA GGGTGAAACT
    GGTCCTATTG GTTCAATTGG TGCTCCTGGT GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG
    ATTGGTGCAC CTGGACCTGC TGGCTTTGCT GGACCCCCTG GTGCTGATGG CCAACCTGGA
    AATAAGGGAG AGCAGGGTGA ATCAGGACAG AAAGGTGATT CAGGAGCCCC TGGACCCCAA
    GGACCATCAG GTGCTCCAGG CCCAGTGGGC CCAACTGGTG TCACAGGACC TAAGGGAGCA
    CGTGGAGCCC AAGGAGCCCC TGGTGCCACT GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA
    CCCCCTGGCC CCAACGGTAA CCCTGGTGCT GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT
    GGTCCTAAGG GAGTTCGTGG TGATGCCGGT CCCCCAGGTA GAGCAGGAGA TGCTGGACTG
    CGTGGACCTC CTGGTGCTCC TGGAGAGAAG GGAGAGGCTG GTGAAGATGG TCCTCCTGGT
    CCTGATGGTC CTTCCGGCCC TGCTGGTCTT GCTGGTCAGC GTGGTATTGT TGGTTTGCCT
    GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA
    CAGGGAGCTC CAGGAGGCTC TGGTGACCGT GGACCTCCTG GCCCTGTTGG CCCACCTGGA
    TTGACTGGAC CTGCTGGAGA GACTGGCCGT GAGGGTAATC CTGGATCTGA TGGTCCACCT
    GGTAGAGATG GTGCTGCTGG TGTGAAGGGT GAGCGTGGTA ACACTGGCCC AATTGGTGCT
    CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT GGTTCAGTTG GTCCAATTGG CAAACAGGGA
    GACAGAGGAG AGAATGGCCC ACAAGGACCT GCTGGACCCC CTGGACCCGC TGGAGCTCGA
    GGCATGGTTG GACCCCAAGG ACCTCGTGGT GATAAGGGTG AGGCAGGAGA AGCTGGAGAA
    AGAGGCCAGA AGGGACACAG AGGATTCACT GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT
    TCTCCTGGAG ACCAGGGTGC TGCTGGACCT GCTGGACCTT CTGGTGCTAA AGGACCTTCA
    GGACCTGTTG GACCTGCTGG TAAGGATGGT TCTAATGGTC AGCCTGGACC CATTGGTCCA
    CCTGGACCTC GTGGACGCTC TGGAGAGTCT GGCCCAGTTG GTCCTCCTGG TAACCCCGGA
    CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT GGCATTGACA TGTCTGCCTT CGCTGGATTG
    TCTCAGCCAG AGAAGGGACC CGATCCTCTG CGTTACATGC GTGCAGATGA AGCCTCCAGC
    TCTCTCAGAC AACACGATGT GGAGGTGGAC GCCACTCTGA AGTCCATCAA CGGGCAGATT
    GAGGATATCC GCAGCCCCGA TGGCTCCCGC AAGAATCCTG CACGCTCCTG CCGAGACCTG
    AAACTCTGCC ACCCTGAATG GAAGAGCGGT GACTACTGGG TGGATCCCAA CCTTGGTAGC
    GCTGCTGATG CCATCAAAGT TTTCTGCAAC ATGGAGACCG GAGAGACCTG TGTGAAACCA
    AGCACTCCTA AAATCCCACG CAAGAACTGG TGGACAAGCA AGAGCAAGGC CCAGAAGCAC
    GTGTGGTTCG GAGAAAGCAT GAACGGCGGA TTCCACTTTA GCTATGCTGA TGGCAGCCAG
    ACTCCTAGTA CTACCACCAT CCAGCTGAAC TTCCTCAGGC TGCTGTCTAC TGAGGCCACT
    CAGACCATCA CCTACCACTG CAAGAACAGC GTGGCTTACA TGGACCAAGC CACAGGCAAC
    CTGAAGAAGG CCATTCTGCT GCAGGGCTCC AATGATGTGG AGATCAGAGC AGAGGGCAAC
    AGCCGCTTTA CATATGGCGT ACTTGAGGAT GGCTGCAAGA AGCACACAGG TCAATGGGCC
    AAGACTGTGA TCGAGTACAA AACTCAGAAG ACATCCAGAC TGCCCATCAT GGACATCGCT
    CCAATGGACA TCGGAGGCGC AGATCAGGAG TTCGGAGTGG ACATTGGCGC AGTCTGCTTC
    TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166920.1
    CDS187..4452
    Translation

    Target ORF information:

    RefSeq Version XM_005166863.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen, type II, alpha 1a (col2a1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166863.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTCAGAT TGCTGGATTC ACGGACTCTC CTGCTACTTG TGGCAACGCA TAGCGTTTTG 
    CTATCGCTTG TAAGATGTCA ACAAGAAGAT GACCGGTCAC TTGGTGCAAA GGGACAGAAA
    GGAGAACCAG GCGATATTAC AGATGTTGTA GGACCAAGAG GACCTGCTGG ACCAATGGGA
    CCCCCGGGAG AGCAGGGAAC ACGTGGAGAA AGAGGAGCTA AGGGTGAGAA GGGAAGTCCC
    GGCCCAAGAG GGAGAGATGG CGAGCCTGGT ACACCTGGAA ACCCAGGACC CCCTGGACCA
    CCCGGACCCA ATGGTCCCCC TGGACTTGGA GGAAACTTTG CTGCTCAGAT GGCTGGTGGT
    TTTGATGAGA AGGCAGGAGG TGCTCAGATG GGTGTGATGC AGGGACCAAT GGGTCCAATG
    GGTCCCCGTG GTCCTCCTGG CCCCTCTGGA GCACCTGGCC CACAAGGTTT CCAAGGCAAC
    CCTGGAGAGA CTGGTGAACC CGGCCCTGCT GGTGCCCTTG GACCCCGTGG CCCACCCGGC
    CCCCCTGGAA AACCCGGAAG TGATGGTGAG GCTGGCAAAC CTGGTAAAGC TGGTGAGCGT
    GGACCCCCAG GACCTCAGGG AGCTCGTGGA TTCCCTGGGA CCCCTGGACT CCCTGGCATC
    AAGGGACACA GAGGACACCC AGGTCTGGAT GGTGCTAAAG GAGAGGCTGG TGCTGCAGGT
    GCCAAGGGTG AGGCAGGTTC TAACGGTGAG AGTGGCGCTC CTGGACCAAT GGGTCCTCGT
    GGTCTGCCCG GTGAGAGAGG TCGCCCTGGT GCAACTGGTG CTGCTGGTGC CCGTGGTAAT
    GATGGTCTGC CTGGTCCTGC TGGTCCTCCT GGTCCTGTTG GTCCTGCTGG TGCTCCAGGT
    TTCCCAGGAT CCCCTGGATC TAAGGGTGAA GCTGGTCCCA CTGGGGCTCG TGGACCTGAG
    GGCGCACAGG GACCTCGTGG AGAGGCTGGT ACACCTGGAT CACCTGGACC CGCCGGTGCA
    TCCGGTAACC CTGGTACTGA TGGTATTCCT GGAGCTAAAG GATCTGCTGG TGCTTCTGGC
    ATTGCTGGTG CTCCTGGTTT CCCAGGACCA CGTGGTCCAC CAGGACCTCA GGGAGCCACT
    GGACCTCTTG GGCCTAAAGG ACAATCTGGA GACCCTGGTA TCCCAGGATT CAAAGGCGAG
    GCTGGACCCA AGGGAGAGCG TGGTGTTCTA GGACCTCAAG GACCTCCTGG GCCATCAGGT
    GAAGAGGGCA AGAGAGGACC AAGAGGAGAG CCTGGTTCTG CCGGACCTCT TGGACCTCCT
    GGAGAGAGAG GAGCTCCAGG TAACCGTGGA TTCCCTGGCC AGGATGGACT TGCAGGAGCA
    AAGGGTGCTC CTGGTGATCG TGGTGTTCCA GGTTTGAGTG GACCGAAAGG AGGCACTGGA
    GATCCTGGTC GCCCTGGTGA ACCTGGTCTT CCTGGAGCCA GAGGTCTCAC TGGACGCCCT
    GGTGATGCTG GAGCTCAAGG AAAAGTCGGA GCGACTGGTG CCCCTGGTGA AGATGGCCGC
    CCTGGACCAC CTGGGCCTCT GGGAGCTCGT GGTCAGCCTG GAGTGATGGG ATTCCCTGGA
    CCCAAGGGAG CCAATGGTGA ACCTGGAAAA CCTGGAGAGA AAGGACTTGT TGGACGCACT
    GGTCTTAGAG GTTTGCCTGG TAAAGATGGA GAGACTGGAC CTTCTGGTCC TCCTGGCCCT
    GTTGGTGCTG TTGGTGAGAG AGGAGAACAA GGTCAACCTG GTCCATCTGG CTTCCAGGGT
    CTGCCTGGAC CTACTGGAGC CCCCGGAGAA CCTGGTAAAC CTGGTGACCA GGGTGTTCCT
    GGAGAGGGTG GTGCTGCCGG TCCCACTGGA CCAAGAGGTG AGCGTGGTTT CCCTGGTGAG
    AGAGGAGGCG CTGGCCCTCA GGGTCTCCAA GGACCAAGAG GACTTCCAGG AACTCCCGGA
    ACAGATGGAC CCAAGGGTGC GATTGGGCCA GCTGGTGCTG CTGGAGCTCA AGGTCCTCCC
    GGTCTTCAAG GCATGCCTGG TGAGAGAGGA GCTGTTGGTA TCTCTGGAGC TAAAGGTGAC
    AGAGGTGACT CTGGAGAGAA GGGACCTGAA GGTGCTCCTG GTAAAGATGG TTCAAGAGGT
    TTGACTGGTC CAATTGGCCC ACCTGGTCCT TCTGGTCCCA ATGGCGCAAA GGGTGAAACT
    GGTCCTATTG GTTCAATTGG TGCTCCTGGT GCTCGTGGTG CCCCTGGTGA TCGTGGTGAG
    ATTGGTGCAC CTGGACCTGC TGGCTTTGCT GGACCCCCTG GTGCTGATGG CCAACCTGGA
    AATAAGGGAG AGCAGGGTGA ATCAGGACAG AAAGGTGATT CAGGAGCCCC TGGACCCCAA
    GGACCATCAG GTGCTCCAGG CCCAGTGGGC CCAACTGGTG TCACAGGACC TAAGGGAGCA
    CGTGGAGCCC AAGGAGCCCC TGGTGCCACT GGTTTCCCAG GTGCTGCAGG ACGAGTTGGA
    CCCCCTGGCC CCAACGGTAA CCCTGGTGCT GCCGGCCCAG CAGGTCCTTC TGGTAAGGAT
    GGTCCTAAGG GAGTTCGTGG TGATGCCGGT CCCCCAGGTA GAGCAGGAGA TGCTGGACTG
    CGTGGACCTC CTGGTGCTCC TGGAGAGAAG GGAGAGGCTG GTGAAGATGG TCCTCCTGGT
    CCTGATGGTC CTTCCGGCCC TGCTGGTCTT GCTGGTCAGC GTGGTATTGT TGGTTTGCCT
    GGTCAGCGTG GTGAAAGAGG TTTCCCTGGA CTTCCAGGAC CTTCTGGTGA GCCTGGCAAA
    CAGGGAGCTC CAGGAGGCTC TGGTGACCGT GGACCTCCTG GCCCTGTTGG CCCACCTGGA
    TTGACTGGAC CTGCTGGAGA GACTGGCCGT GAGGGTAATC CTGGATCTGA TGGTCCACCT
    GGTAGAGATG GTGCTGCTGG TGTGAAGGGT GAGCGTGGTA ACACTGGCCC AATTGGTGCT
    CCTGGAGCTC CTGGTGCCCC TGGCGCTCCT GGTTCAGTTG GTCCAATTGG CAAACAGGGA
    GACAGAGGAG AGAATGGCCC ACAAGGACCT GCTGGACCCC CTGGACCCGC TGGAGCTCGA
    GGCATGGTTG GACCCCAAGG ACCTCGTGGT GATAAGGGTG AGGCAGGAGA AGCTGGAGAA
    AGAGGCCAGA AGGGACACAG AGGATTCACT GGTCTGCAGG GTCTGCCCGG ACCTCCCGGT
    TCTCCTGGAG ACCAGGGTGC TGCTGGACCT GCTGGACCTT CTGGTGCTAA AGGACCTTCA
    GGACCTGTTG GACCTGCTGG TAAGGATGGT TCTAATGGTC AGCCTGGACC CATTGGTCCA
    CCTGGACCTC GTGGACGCTC TGGAGAGTCT GGCCCAGTTG GTCCTCCTGG TAACCCCGGA
    CCCCCTGGTC CTCCTGGTCC CCCTGGCCCT GGCATTGACA TGTCTGCCTT CGCTGGATTG
    TCTCAGCCAG AGAAGGGACC CGATCCTCTG CGTTACATGC GTGCAGATGA AGCCTCCAGC
    TCTCTCAGAC AACACGATGT GGAGGTGGAC GCCACTCTGA AGTCCATCAA CGGGCAGATT
    GAGGATATCC GCAGCCCCGA TGGCTCCCGC AAGAATCCTG CACGCTCCTG CCGAGACCTG
    AAACTCTGCC ACCCTGAATG GAAGAGCGGT GACTACTGGG TGGATCCCAA CCTTGGTAGC
    GCTGCTGATG CCATCAAAGT TTTCTGCAAC ATGGAGACCG GAGAGACCTG TGTGAAACCA
    AGCACTCCTA AAATCCCACG CAAGAACTGG TGGACAAGCA AGAGCAAGGC CCAGAAGCAC
    GTGTGGTTCG GAGAAAGCAT GAACGGCGGA TTCCACTTTA GCTATGCTGA TGGCAGCCAG
    ACTCCTAGTA CTACCACCAT CCAGCTGAAC TTCCTCAGGC TGCTGTCTAC TGAGGCCACT
    CAGACCATCA CCTACCACTG CAAGAACAGC GTGGCTTACA TGGACCAAGC CACAGGCAAC
    CTGAAGAAGG CCATTCTGCT GCAGGGCTCC AATGATGTGG AGATCAGAGC AGAGGGCAAC
    AGCCGCTTTA CATATGGCGT ACTTGAGGAT GGCTGCAAGA AGCACACAGG TCAATGGGCC
    AAGACTGTGA TCGAGTACAA AACTCAGAAG ACATCCAGAC TGCCCATCAT GGACATCGCT
    CCAATGGACA TCGGAGGCGC AGATCAGGAG TTCGGAGTGG ACATTGGCGC AGTCTGCTTC
    TTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.