kl cDNA ORF clone, Danio rerio(zebrafish)

The following kl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa16317 XM_685705.6
Latest version!
Danio rerio klotho (kl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa16317 XM_021479418.1
Latest version!
Danio rerio klotho (kl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa43787 XM_009305512.2
Latest version!
Danio rerio klotho (kl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa145174 XM_685705.7
Latest version!
Danio rerio klotho (kl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa16317
    Clone ID Related Accession (Same CDS sequence) XM_685705.6 , XM_021479418.1
    Accession Version XM_685705.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product klotho isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685705.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATTAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GGATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACTAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACAAGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ATACTACACC TACGCAGTTC ACAGACACTA ATGTTTACGT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGC CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACCG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCAACG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CCATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCTA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTGATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAGG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAA GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690797.4
    CDS27..2999
    Translation

    Target ORF information:

    RefSeq Version XM_685705.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio klotho (kl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685705.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATTAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GGATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACTAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACAAGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ATACTACACC TACGCAGTTC ACAGACACTA ATGTTTACGT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGC CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACCG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCAACG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CCATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCTA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTGATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAGG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAA GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16317
    Clone ID Related Accession (Same CDS sequence) XM_685705.6 , XM_021479418.1
    Accession Version XM_021479418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product klotho
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATTAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GGATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACTAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACAAGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ATACTACACC TACGCAGTTC ACAGACACTA ATGTTTACGT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGC CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACCG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCAACG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CCATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCTA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTGATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAGG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAA GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335093.1
    CDS51..3023
    Translation

    Target ORF information:

    RefSeq Version XM_021479418.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio klotho (kl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATTAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GGATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACTAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACAAGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ATACTACACC TACGCAGTTC ACAGACACTA ATGTTTACGT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGC CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACCG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCAACG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CCATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCTA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTGATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAGG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAA GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43787
    Clone ID Related Accession (Same CDS sequence) XM_009305512.2
    Accession Version XM_009305512.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product klotho isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGGCCG TCGGCACCGC TGCATATTCA GTGGAAGGAG CCTGGGAAAA AGACGGGAAG 
    GGTAAGTCAA TCTGGGACAC TTTTACTCGA GGAGGGACCC GAGTGTCCAG AGGAGACGTT
    GGTAGCGATA GTTATCATAA CATCCCTGGA GACCTTCGAG CTCTTCAGCA ACTAGGAGTC
    AGTCATTATC GGTTCTCCTT GTCCTGGCCA CGCATTTTCT CCAATGGCAC TAAGGAAAGC
    TACAACGACA AAGGTGTGGA GTATTATAAA AATCTGATCC GGGGTTTGAA GGACATTAAG
    GTGCAGCCTG TTGTCACTTT GTATCATTGG GACCTGCCTG ACAGTTTGCA GACGCTCTTT
    GGAGGCTGGA GTAATTCGGT TATGGTGGAG CTGTTCAGAG ACTATGCCGA TTTTTGCTTT
    AAGACATTTG GATCGGATGT GAAATTTTGG ATCACTATCG ACAATCCTTT TGTCGTGGCC
    TGGCATGGAT ATGGAACTGG GGTTGTGGCT CCGGGTATTA AGAATGACTC TGATTTGCCT
    TTCAGAGTCG GACACAATCT GCTGAAGGCT CACGCTGCAG CCTGGCACCT GTATGACGAG
    CGATATCGTG CCGCTCAGGG TGGGAGAGTG TCCATGGCTC TGGGATCTCA CTGGATCAAA
    CCCAGCAGAA CCAGACAGGA GAGCCGCAAA GCCTGCCAGC GCTCTCTAAA CTTTGTGCTT
    GGCTGGTTCG CCCGTCCCTT GTTTGTAGAT GGAGATTATC CACCCTGCAT GAAGGACAAT
    CTGACCCACA GACTGCCGTC CTTCACCGAG GCCGAGAGCG CGTATGTGAA CGGAACCGCC
    GACTTCTTTG CTCTGTCTCA TGGACCTGCT CTTAGTTTTC AGCTGATCAA TGACAGTCTG
    CGCTTCGGCC AGACTGAAGA TTTGGGTCTG AGGATGCTTT TGTACTGGGT CCGTGCAGAG
    TACAACAATC CTCCCATCTT TGTGGTGGAG AGCGGCTGGT ATGGAAGTGG CAACACTAAA
    ACAAAGGATG CCAAGCATAT GTACTATTTA AAGCGCTTCA TTATGGAGAC GCTAAAAGCA
    ATCCATGTTG ACAGGGTGAA TGTGATTGGC TACACGGCCT GGTCTCTGCT GGATGGATAT
    GAGTGGTACC GTGAATATGC GATACGCAGA GGGCTGTTCT ATGTGGATTT CAACACTCCT
    GATCTGAAGA GAGAGCCGAA GGCATCTGCC ACTTTCTATA GCAAACTCAT AGAGAAGAAT
    GGCTTCCCTC AGCTGCCAGA GAACCGCCCT GCACAAGGTG CATTTCCCTG TGACTTTGCT
    TGGGGAGTGG CTGCCAACTC CATACAGGTT GATACTACAC CTACGCAGTT CACAGACACT
    AATGTTTACG TCTGGAACAT TTCTGGAAAC GGAGAGCTGA AAAAGCTCCC TGGGCTGCAG
    GCGCCCCATC TGCGCAGGAC ACCCCACTGC GCCGACTACG GCAGCATCCG CCAGCAGGTG
    TCTGATCTAC TCCGAATGCA AGTCTCCCAT TTCCATTTCT CCCTCAACTG GTCCTCCATC
    GTCCCCACAG GCCACGTGTC TGATGCTAAC GAAACATTGC TAAGATACTA TTATTGTTTT
    GTCAGCGAGC TACAAAAAGT GAACATAACC CCTGTGGTGA CTCTTTGGCA CCATACAGGG
    AAACTGTCCA GCCTTCCAGC ACCAATGGAG GCCAGTGATG GCTGGCAGAG CGAGAAGACC
    GTCCAGGCCT TTGTGGACTA TGCTAGGTTG TGTTTCCAAC GACTAGGAGC TCATGTCAAG
    CTCTGGATCA CACTCAATGA GCCCAACGAT GAGGATCTTG AATACACTGT AGGTCACCAG
    CTGCTACGTG CACATGCTCT AGCTTGGCAC GTCTACGACA GAGAATTCCG CAAGGCCCAA
    GGTGGCAAAG CATCACTGGT TCTTCATATG GATTGGGTCG AACCTGCATT TTCATTCAAC
    AGGGAGGACG TGGCCCCTGC AGACCGAGTC CTGGACTTTC GAGTGGGATG GTTTGCGGAG
    CCCATTTTCG GTAAAGGCGA TTACCCGGCG GTGATGCGTA GCTGGCTCCA ACAGAGGAAC
    ACTATTGATC TTTTCAACTA CCACTTACCT ACGTTCAGCG AAGAGGATCG GCTGTTGGTG
    AAAGGAACCT ACGATTTCTT CGCCATCAGC CATTTTACTA CCTCGATGGT GTACGATGGA
    GTGGAGGATA AATACACCTT TAAGGACAAG CTGCAGGTTC AGCTGATATC TGACGTGACC
    TGGATCATGT CCCCAAGACG CAACTCTCCT GTGGTTCCCT GGGGTCTACG CAAGGCATTG
    AACTGGGTGA ACTCGCGATA CAAAGGTGTT CCCATTTACG TGATGGCCAA TGGTGTTCAA
    GAGGATACCG CTAGGTTTAG GGATAGCTTG CGCAGCTATT ACCTTTACAA CTATGTGAAT
    GAGGCCTTGA AAGCATACAT GCTGGATGCA GTGAATCTAA AAGGCTATTT TGCATACGCA
    TTCAGTGACC AGCGGGACCC AGGCTTTGGC ATGTATGGTC ACGTGCAGGA AGAGGTGATT
    TCAAAATCCT CACTGGGCCA TTACAAAAAC ATTATCCGCC ACAATGGCTT CCCAGCCCCA
    AGCACATCTC AGCATCAGTG TCCCCATGCT CCAGCTCAAG GATCGGGCCG ATATGTCCTC
    ACCAAGAAGC CTGTGGTCGG CTTTCTCTCT CTGGTGAGCT CCTGCATGCT CATCACTATG
    TGCCTTGTTA TCTACTATGC ATTTAAGAGG CACAAGTTAA CCACCAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009303787.1
    CDS258..3071
    Translation

    Target ORF information:

    RefSeq Version XM_009305512.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio klotho (kl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305512.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGGCCG TCGGCACCGC TGCATATTCA GTGGAAGGAG CCTGGGAAAA AGACGGGAAG 
    GGTAAGTCAA TCTGGGACAC TTTTACTCGA GGAGGGACCC GAGTGTCCAG AGGAGACGTT
    GGTAGCGATA GTTATCATAA CATCCCTGGA GACCTTCGAG CTCTTCAGCA ACTAGGAGTC
    AGTCATTATC GGTTCTCCTT GTCCTGGCCA CGCATTTTCT CCAATGGCAC TAAGGAAAGC
    TACAACGACA AAGGTGTGGA GTATTATAAA AATCTGATCC GGGGTTTGAA GGACATTAAG
    GTGCAGCCTG TTGTCACTTT GTATCATTGG GACCTGCCTG ACAGTTTGCA GACGCTCTTT
    GGAGGCTGGA GTAATTCGGT TATGGTGGAG CTGTTCAGAG ACTATGCCGA TTTTTGCTTT
    AAGACATTTG GATCGGATGT GAAATTTTGG ATCACTATCG ACAATCCTTT TGTCGTGGCC
    TGGCATGGAT ATGGAACTGG GGTTGTGGCT CCGGGTATTA AGAATGACTC TGATTTGCCT
    TTCAGAGTCG GACACAATCT GCTGAAGGCT CACGCTGCAG CCTGGCACCT GTATGACGAG
    CGATATCGTG CCGCTCAGGG TGGGAGAGTG TCCATGGCTC TGGGATCTCA CTGGATCAAA
    CCCAGCAGAA CCAGACAGGA GAGCCGCAAA GCCTGCCAGC GCTCTCTAAA CTTTGTGCTT
    GGCTGGTTCG CCCGTCCCTT GTTTGTAGAT GGAGATTATC CACCCTGCAT GAAGGACAAT
    CTGACCCACA GACTGCCGTC CTTCACCGAG GCCGAGAGCG CGTATGTGAA CGGAACCGCC
    GACTTCTTTG CTCTGTCTCA TGGACCTGCT CTTAGTTTTC AGCTGATCAA TGACAGTCTG
    CGCTTCGGCC AGACTGAAGA TTTGGGTCTG AGGATGCTTT TGTACTGGGT CCGTGCAGAG
    TACAACAATC CTCCCATCTT TGTGGTGGAG AGCGGCTGGT ATGGAAGTGG CAACACTAAA
    ACAAAGGATG CCAAGCATAT GTACTATTTA AAGCGCTTCA TTATGGAGAC GCTAAAAGCA
    ATCCATGTTG ACAGGGTGAA TGTGATTGGC TACACGGCCT GGTCTCTGCT GGATGGATAT
    GAGTGGTACC GTGAATATGC GATACGCAGA GGGCTGTTCT ATGTGGATTT CAACACTCCT
    GATCTGAAGA GAGAGCCGAA GGCATCTGCC ACTTTCTATA GCAAACTCAT AGAGAAGAAT
    GGCTTCCCTC AGCTGCCAGA GAACCGCCCT GCACAAGGTG CATTTCCCTG TGACTTTGCT
    TGGGGAGTGG CTGCCAACTC CATACAGGTT GATACTACAC CTACGCAGTT CACAGACACT
    AATGTTTACG TCTGGAACAT TTCTGGAAAC GGAGAGCTGA AAAAGCTCCC TGGGCTGCAG
    GCGCCCCATC TGCGCAGGAC ACCCCACTGC GCCGACTACG GCAGCATCCG CCAGCAGGTG
    TCTGATCTAC TCCGAATGCA AGTCTCCCAT TTCCATTTCT CCCTCAACTG GTCCTCCATC
    GTCCCCACAG GCCACGTGTC TGATGCTAAC GAAACATTGC TAAGATACTA TTATTGTTTT
    GTCAGCGAGC TACAAAAAGT GAACATAACC CCTGTGGTGA CTCTTTGGCA CCATACAGGG
    AAACTGTCCA GCCTTCCAGC ACCAATGGAG GCCAGTGATG GCTGGCAGAG CGAGAAGACC
    GTCCAGGCCT TTGTGGACTA TGCTAGGTTG TGTTTCCAAC GACTAGGAGC TCATGTCAAG
    CTCTGGATCA CACTCAATGA GCCCAACGAT GAGGATCTTG AATACACTGT AGGTCACCAG
    CTGCTACGTG CACATGCTCT AGCTTGGCAC GTCTACGACA GAGAATTCCG CAAGGCCCAA
    GGTGGCAAAG CATCACTGGT TCTTCATATG GATTGGGTCG AACCTGCATT TTCATTCAAC
    AGGGAGGACG TGGCCCCTGC AGACCGAGTC CTGGACTTTC GAGTGGGATG GTTTGCGGAG
    CCCATTTTCG GTAAAGGCGA TTACCCGGCG GTGATGCGTA GCTGGCTCCA ACAGAGGAAC
    ACTATTGATC TTTTCAACTA CCACTTACCT ACGTTCAGCG AAGAGGATCG GCTGTTGGTG
    AAAGGAACCT ACGATTTCTT CGCCATCAGC CATTTTACTA CCTCGATGGT GTACGATGGA
    GTGGAGGATA AATACACCTT TAAGGACAAG CTGCAGGTTC AGCTGATATC TGACGTGACC
    TGGATCATGT CCCCAAGACG CAACTCTCCT GTGGTTCCCT GGGGTCTACG CAAGGCATTG
    AACTGGGTGA ACTCGCGATA CAAAGGTGTT CCCATTTACG TGATGGCCAA TGGTGTTCAA
    GAGGATACCG CTAGGTTTAG GGATAGCTTG CGCAGCTATT ACCTTTACAA CTATGTGAAT
    GAGGCCTTGA AAGCATACAT GCTGGATGCA GTGAATCTAA AAGGCTATTT TGCATACGCA
    TTCAGTGACC AGCGGGACCC AGGCTTTGGC ATGTATGGTC ACGTGCAGGA AGAGGTGATT
    TCAAAATCCT CACTGGGCCA TTACAAAAAC ATTATCCGCC ACAATGGCTT CCCAGCCCCA
    AGCACATCTC AGCATCAGTG TCCCCATGCT CCAGCTCAAG GATCGGGCCG ATATGTCCTC
    ACCAAGAAGC CTGTGGTCGG CTTTCTCTCT CTGGTGAGCT CCTGCATGCT CATCACTATG
    TGCCTTGTTA TCTACTATGC ATTTAAGAGG CACAAGTTAA CCACCAAAAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145174
    Clone ID Related Accession (Same CDS sequence) XM_685705.7
    Accession Version XM_685705.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product klotho
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685705.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATCAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GAATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACCAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACACGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ACACTACCCC TACGCAGTTC ACCGACACTA ATGTTTACAT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGT CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACTG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCATCG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CTATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCCA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTTATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAAG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAG GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690797.5
    CDS51..3023
    Translation

    Target ORF information:

    RefSeq Version XM_685705.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio klotho (kl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685705.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAAGTGA CATGGATTCC TCTGCCGCTC GTTTTGTTCT GTCAATTTCA GGGCACAGCA 
    TCTGATCCAG GAGCAGGACA GCACACATGG GACACATTCA GCAAACTTCC ATATCCCGAC
    GACAAAGCCT TCCTCTATGA CACCTTTCCT GATAAGTTTA TGTGGGCCGT CGGCACCGCT
    GCATATTCAG TGGAAGGAGC CTGGGAAAAA GACGGGAAGG GTAAGTCAAT CTGGGACACT
    TTTACTCGAG GAGGGACCCG AGTGTCCAGA GGAGACGTTG GTAGCGATAG TTATCATAAC
    ATCCCTGGAG ACCTTCGAGC TCTTCAGCAA CTAGGAGTCA GTCATTATCG GTTCTCCTTG
    TCCTGGCCAC GCATTTTCTC CAATGGCACT AAGGAAAGCT ACAACGACAA AGGTGTGGAG
    TATTATAAAA ATCTGATCCG GGGTTTGAAG GACATTAAGG TGCAGCCTGT TGTCACTTTG
    TATCATTGGG ACCTGCCTGA CAGTTTGCAG ACGCTCTTTG GAGGCTGGAG TAATTCGGTT
    ATGGTGGAGC TGTTCAGAGA CTATGCCGAT TTTTGCTTTA AGACATTTGG ATCGGATGTG
    AAATTTTGGA TCACTATCGA CAATCCTTTT GTCGTGGCCT GGCATGGATA TGGAACTGGG
    GTTGTGGCTC CGGGTATCAA GAATGACTCT GATTTGCCTT TCAGAGTCGG ACACAATCTG
    CTGAAGGCTC ACGCTGCAGC CTGGCACCTG TATGACGAGC GATATCGTGC CGCTCAGGGT
    GGGAGAGTGT CCATGGCTCT GGGATCTCAC TGGATCAAAC CCAGCAGAAC CAGACAGGAG
    AGCCGCAAAG CCTGCCAGCG CTCTCTAAAC TTTGTGCTTG GCTGGTTCGC CCGTCCCTTG
    TTTGTAGATG GAGATTATCC ACCCTGCATG AAGGACAATC TGACCCACAG ACTGCCGTCC
    TTCACCGAGG CCGAGAGCGC GTATGTGAAC GGAACCGCCG ACTTCTTTGC TCTGTCTCAT
    GGACCTGCTC TTAGTTTTCA GCTGATCAAT GACAGTCTGC GCTTCGGCCA GACTGAAGAT
    TTGGGTCTGA GAATGCTTTT GTACTGGGTC CGTGCAGAGT ACAACAATCC TCCCATCTTT
    GTGGTGGAGA GCGGCTGGTA TGGAAGTGGC AACACCAAAA CAAAGGATGC CAAGCATATG
    TACTATTTAA AGCGCTTCAT TATGGAGACG CTAAAAGCAA TCCATGTTGA CAGGGTGAAT
    GTGATTGGCT ACACGGCCTG GTCTCTGCTG GATGGATATG AGTGGTACCG TGAATATGCG
    ATACGCAGAG GGCTGTTCTA TGTGGATTTC AACACTCCTG ATCTGAAGAG AGAGCCGAAG
    GCATCTGCCA CTTTCTATAG CAAACTCATA GAGAAGAATG GCTTCCCTCA GCTGCCAGAG
    AACCGCCCTG CACACGGTGC ATTTCCCTGT GACTTTGCTT GGGGAGTGGC TGCCAACTCC
    ATACAGGTTG ACACTACCCC TACGCAGTTC ACCGACACTA ATGTTTACAT CTGGAACATT
    TCTGGAAACG GAGAGCTGAA AAAGCTCCCT GGGCTGCAGG CGCCCCATCT GCGCAGGACA
    CCCCACTGCG CCGACTACGG CAGCATCCGT CAGCAGGTGT CTGATCTACT CCGAATGCAA
    GTCTCCCATT TCCATTTCTC CCTCAACTGG TCCTCCATCG TCCCCACAGG CCACGTGTCT
    GATGCTAACG AAACATTGCT AAGATACTAT TATTGTTTTG TCAGCGAGCT ACAAAAAGTG
    AACATAACCC CTGTGGTGAC TCTTTGGCAC CATACAGGGA AACTGTCCAG CCTTCCAGCA
    CCAATGGAGG CCAGTGATGG CTGGCAGAGC GAGAAGACTG TCCAGGCCTT TGTGGACTAT
    GCTAGGTTGT GTTTCCATCG ACTAGGAGCT CATGTCAAGC TCTGGATCAC ACTCAATGAG
    CCCAACGATG AGGATCTTGA ATACACTGTA GGTCACCAGC TGCTACGTGC ACATGCTCTA
    GCTTGGCACG TCTACGACAG AGAATTCCGC AAGGCCCAAG GTGGCAAAGC ATCACTGGTT
    CTTCATATGG ATTGGGTCGA ACCTGCATTT TCATTCAACA GGGAGGACGT GGCCCCTGCA
    GACCGAGTCC TGGACTTTCG AGTGGGATGG TTTGCGGAGC CTATTTTCGG TAAAGGCGAT
    TACCCGGCGG TGATGCGTAG CTGGCTCCAA CAGAGGAACA CTATTGATCT TTTCAACTAC
    CACTTACCCA CGTTCAGCGA AGAGGATCGG CTGTTGGTGA AAGGAACCTA CGATTTCTTC
    GCCATCAGCC ATTTTACTAC CTCGATGGTG TACGATGGAG TGGAGGATAA ATACACCTTT
    AAGGACAAGC TGCAGGTTCA GCTTATATCT GACGTGACCT GGATCATGTC CCCAAGACGC
    AACTCTCCTG TGGTTCCCTG GGGTCTACGC AAGGCATTGA ACTGGGTGAA CTCGCGATAC
    AAAGGTGTTC CCATTTACGT GATGGCCAAT GGTGTTCAAG AGGATACCGC TAGGTTTAAG
    GATAGCTTGC GCAGCTATTA CCTTTACAAC TATGTGAATG AGGCCTTGAA AGCATACATG
    CTGGATGCAG TGAATCTAAA AGGCTATTTT GCATACGCAT TCAGTGACCA GCGGGACCCA
    GGCTTTGGCA TGTATGGTCA CGTGCAGGAA GAGGTGATTT CAAAATCCTC ACTGGGCCAT
    TACAAAAACA TTATCCGCCA CAATGGCTTC CCAGCCCCAG GCACATCTCA GCATCAGTGT
    CCCCATGCTC CAGCTCAAGG ATCGGGCCGA TATGTCCTCA CCAAGAAGCC TGTGGTCGGC
    TTTCTCTCTC TGGTGAGCTC CTGCATGCTC ATCACTATGT GCCTTGTTAT CTACTATGCA
    TTTAAGAGGC ACAAGTTAAC CACCAAAAAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.