PIK3CA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIK3CA
Entrez Gene ID	561737
Full Name	phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
General protein information	Preferred Names phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha Names phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306176.2	XP_009304451.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_009306176.3	XP_009304451.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_009306177.3	XP_009304452.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_009306177.2	XP_009304452.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_021479874.1	XP_021335549.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_005155854.3	XP_005155911.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
XM_017358348.1	XP_017213837.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC561737	XP_690235.2
Xenopus tropicalis (tropical clawed frog)	pik3ca	NP_001119972.1
Canis lupus familiaris (dog)	PIK3CA	XP_545208.2
	PIK3CA	XP_001109162.1
Bos taurus (cattle)	PIK3CA	NP_776999.1
Mus musculus (house mouse)	Pik3ca	NP_032865.2
Rattus norvegicus (Norway rat)	Pik3ca	NP_596890.2
Pan troglodytes (chimpanzee)	PIK3CA	XP_001167506.1
Danio rerio (zebrafish)	LOC100334726	XP_005174483.1
Homo sapiens (human)	PIK3CA	NP_006209.2
Gallus gallus (chicken)	PIK3CA	NP_001004410.1
Caenorhabditis elegans (roundworm)	age-1	NP_496462.2

Gene Ontology

The following PIK3CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIK3CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17243	XM_021479874.1 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17243	XM_009306177.3 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17243	XM_005155854.3 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17243	XM_009306176.3 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17243	XM_017358348.1 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa17243	XM_009306176.2	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17243	XM_009306177.2	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIK3CA ( XM_009306176.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304451.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_009306176.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304451.1
CDS	576..3785
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_009306176.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306176.2

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_009306176.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304451.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_009306176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306176.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304451.1
CDS	520..3729
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_009306176.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306176.3

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_009306177.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304452.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_009306177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304452.1
CDS	647..3856
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_009306177.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306177.3

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_009306177.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304452.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_009306177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304452.1
CDS	826..4035
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_009306177.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306177.2

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_021479874.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335549.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.

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CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_021479874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335549.1
CDS	747..3956
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_021479874.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479874.1

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_005155854.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155911.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_005155854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155911.1
CDS	523..3732
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_005155854.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155854.3

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIK3CA ( XM_017358348.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213837.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17243
Clone ID Related Accession (Same CDS sequence)	XM_009306176.2 , XM_009306176.3 , XM_009306177.3 , XM_009306177.2 , XM_021479874.1 , XM_005155854.3 , XM_017358348.1
Accession Version	XM_017358348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213837.1
CDS	573..3782
Translation	MPPRPSSGELWGIHLMPPRILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGFAIGMPVCEFDLVKDPEVQDFRRNILNVCKDSVELRDASGAHSRALYVYPPNVESSPELPKHIYGKLDKGQIIVVIWVIVSPNNDKQKYTLKINHDCVPEQVIAEAIRKKTRSMLLSAEQLKMCVQEYQGKYILKVCGCDEYLLEKYPISQYKYVRSCIMLGRLPNLMLMSKDSLYSQLPMDNFTMPSYSRRISTATPYMNGEASTKSLWTINSTLRIRVLCATYVNVNIRDIDKIYVRTGIYHGGEQLCDNVNTQRVPCSNPRWNEWLTYDMYIPDIPRAARLCLSICSVKGRKGAKEEHCPLAWGNVNLFDYTHTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDYFSSPVKFPDMATIEDHANWIISREMGYNYCQSGQSSRVARDHAMTEADIEQLRQLGNRDPLSEITEQEKDFLWRHRQYCVNIPEILPKILLAVKWNSRDEVAQMYCLLKDWPAIKPEQAMELLDCNYPDPMIRDFAVRCLEKYLTDDKLSQYLIQLVQVLKYEQYLDNPLVRFLLKKALTNQRIGHFFFWHLKSEMHNKTVSQRFGLLLESYCRACGMYLKHLSRQVEAMEKLINLTDILKQEKKDETQKVQMKFLVEQMRRPDYMDALQNFTSPLNPAHQLGNLRLEDCRIMSSAKRPLWLNWENPDIMSELLFQNNEIIFKNGDDLRQDMLTLQIIRIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVRSSHTIMQIQCKGGLKGALQFNSTTLHQWLKDKNKGEMYDMAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKDDGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGGTQECTKTREFERFQEMCYKAYLAIRQHANLFINLFSMMLGSGMPELQSFDDIAYIRKTLALDKTEQEALDYFMKQMNDAHHGGWTTKMDWIFHTIRHHAQN

Target ORF information:

RefSeq Version	XM_017358348.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (pik3ca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358348.1

ORF Insert Sequence:

ATGCCTCCAA GACCCTCTTC GGGAGAGTTA TGGGGCATCC ATTTGATGCC CCCCAGGATT 
TTGGTGGACT GTCTGCTGCC CAATGGCATG ATCCTGACAC TAGAGTGCCT CCGGGAGGCT 
ACGCTCATAA CAATCAAACA TGAACTGTTT AAGGAGGCTA GGAAATACCC CCTACACCAT 
CTGCTGCAGG AGGAGACCTC TTATATTTTC GTCAGTGTAA CGCAAGAGGC AGAGCGAGAG 
GAGTTCTACG ATGAGACCAG AAGACTCTGC GACCTTCGGC TTTTCCAGCC ATTCCTAAAA 
GTTATCGAAC CTGTAGGGAA CAGAGAGGAA AAGATCCTCA ACAGAGAAAT TGGTTTTGCC 
ATTGGCATGC CAGTGTGTGA GTTTGATCTA GTGAAGGATC CTGAGGTGCA GGACTTCAGG 
AGAAATATCC TTAATGTCTG CAAGGACTCA GTAGAGTTGC GGGACGCCAG CGGGGCACAC 
AGTCGAGCAC TATACGTTTA TCCTCCTAAC GTGGAGTCTT CACCAGAGCT TCCAAAGCAT 
ATATATGGCA AATTAGACAA AGGTCAAATC ATTGTGGTCA TCTGGGTGAT CGTCTCGCCC 
AACAACGACA AGCAGAAGTA CACACTAAAG ATCAATCACG ACTGCGTACC TGAGCAGGTG 
ATCGCCGAAG CCATCAGGAA GAAAACCAGA AGCATGCTGC TGTCAGCCGA GCAGCTAAAG 
ATGTGCGTGC AGGAATATCA GGGCAAATAC ATCCTGAAGG TCTGCGGCTG TGACGAGTAT 
CTTTTGGAAA AGTACCCCAT CAGTCAGTAC AAGTATGTAC GGAGTTGCAT TATGCTGGGT 
CGCTTGCCAA ACCTCATGCT TATGTCCAAG GACAGTCTTT ACTCTCAGCT GCCGATGGAC 
AACTTCACCA TGCCCTCCTA TTCCCGCCGC ATCTCCACCG CCACGCCTTA CATGAACGGA 
GAGGCCTCCA CCAAATCTCT CTGGACCATT AACAGCACCC TTAGGATACG GGTCCTCTGC 
GCAACATACG TTAATGTGAA CATTCGGGAC ATTGATAAGA TTTATGTGCG GACGGGGATC 
TACCATGGTG GAGAACAGTT GTGTGATAAT GTCAACACGC AGCGTGTCCC GTGTTCAAAT 
CCGAGGTGGA ACGAGTGGCT GACTTATGAC ATGTACATTC CTGACATCCC CCGGGCGGCT 
CGTCTCTGTC TCTCCATCTG TTCTGTGAAA GGAAGGAAGG GGGCAAAAGA GGAGCACTGT 
CCACTGGCCT GGGGAAACGT CAACCTGTTC GACTACACAC ACACTCTGGT CTCAGGAAAG 
ATGGCACTGA ATCTGTGGCC TGTACCACAC GGCCTAGAAG ACCTGCTTAA CCCCATCGGT 
GTCACAGGCT CCAACCCTAA CAAGGAAACC CCATGTTTAG AGCTGGAGTT TGACTATTTC 
AGCTCACCTG TTAAGTTCCC TGACATGGCT ACAATTGAGG ATCACGCTAA CTGGATAATA 
TCCAGAGAGA TGGGATACAA TTACTGCCAG TCTGGACAGA GCAGTAGAGT GGCACGAGAC 
CATGCAATGA CAGAGGCAGA TATAGAGCAA CTTCGGCAGC TGGGAAACAG GGACCCGCTT 
TCTGAGATCA CTGAGCAGGA AAAAGACTTC CTATGGAGGC ACAGGCAGTA TTGTGTAAAT 
ATACCAGAAA TCCTGCCCAA GATTCTGCTG GCGGTGAAGT GGAATTCCAG AGATGAAGTT 
GCACAGATGT ATTGCCTTCT GAAAGATTGG CCTGCAATAA AGCCGGAACA AGCCATGGAG 
TTGCTTGACT GTAACTACCC TGATCCAATG ATCCGAGACT TTGCGGTTCG GTGCCTTGAG 
AAGTATCTAA CCGATGACAA ACTCTCGCAG TACCTCATTC AATTGGTTCA GGTTTTGAAG 
TACGAGCAGT ATCTTGATAA CCCACTGGTG CGATTTCTGC TAAAAAAAGC TCTCACCAAC 
CAGAGGATTG GGCACTTCTT CTTTTGGCAT TTGAAGTCAG AGATGCACAA TAAAACTGTG 
AGTCAGCGGT TTGGCCTACT GCTGGAGTCG TACTGCCGCG CGTGTGGCAT GTACCTGAAG 
CACCTGAGCA GACAAGTGGA GGCCATGGAG AAGCTCATCA ACCTCACAGA CATTCTCAAA 
CAGGAGAAGA AGGATGAAAC TCAGAAGGTG CAAATGAAGT TTCTAGTGGA GCAGATGAGG 
CGACCAGACT ACATGGATGC CCTCCAAAAT TTCACCTCTC CACTAAACCC TGCCCACCAG 
CTGGGGAACC TACGACTTGA AGACTGCAGA ATCATGTCCT CTGCCAAGAG ACCGCTCTGG 
CTGAACTGGG AAAATCCTGA CATCATGTCC GAACTTCTCT TCCAAAACAA TGAGATCATC 
TTCAAGAACG GAGATGATCT GCGGCAAGAC ATGTTGACGC TTCAGATCAT CAGAATAATG 
GAGAACATTT GGCAAAATCA GGGTCTGGAC CTGAGAATGC TCCCCTATGG CTGCTTGTCT 
ATTGGAGATT GTGTGGGTCT CATCGAGGTG GTAAGAAGTT CTCACACCAT CATGCAGATT 
CAATGTAAAG GAGGCCTGAA GGGTGCGCTG CAGTTTAACA GTACAACGCT GCATCAGTGG 
CTAAAAGACA AGAACAAGGG TGAAATGTAC GACATGGCTA TAGACTTGTT TACGCGATCC 
TGTGCTGGCT ATTGTGTGGC CACTTTCATT CTGGGTATTG GTGACAGGCA CAACAGTAAC 
ATCATGGTGA AGGATGATGG GCAGCTGTTT CATATAGATT TTGGTCACTT TTTGGACCAC 
AAGAAGAAGA AGTTTGGCTA CAAGCGAGAG CGAGTGCCGT TCGTCTTGAC TCAAGACTTC 
CTAATTGTGA TCAGCAAAGG AGGAACCCAG GAGTGCACTA AAACCAGAGA GTTTGAGAGG 
TTTCAAGAGA TGTGCTACAA GGCATACCTG GCCATCCGAC AGCATGCCAA CCTCTTCATC 
AACCTCTTCT CCATGATGCT GGGCTCAGGA ATGCCGGAGC TGCAGTCCTT CGACGACATC 
GCTTACATCC GCAAGACTCT GGCATTAGAC AAAACCGAGC AGGAAGCCCT GGACTATTTC 
ATGAAGCAGA TGAATGACGC TCACCATGGC GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCC GTCACCATGC CCAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PIK3CA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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