vps35 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps35
Entrez Gene ID	561697
Full Name	vacuolar protein sorting 35 homolog (S. cerevisiae)
Synonyms	bZ34G2.3,si:rp71-34g2.3,wu:fa66e03,wu:fb95c08,zgc:136268
General protein information	Preferred Names vacuolar protein sorting 35 homolog (S. cerevisiae) Names vacuolar protein sorting-associated protein 35
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025517.2	NP_001020688.2	vacuolar protein sorting-associated protein 35

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS35	NP_060676.2
Canis lupus familiaris (dog)	VPS35	XP_532570.3
Eremothecium gossypii	AGOS_AEL107W	NP_984754.1
Arabidopsis thaliana (thale cress)	VPS35B	NP_177713.3
Rattus norvegicus (Norway rat)	Vps35	NP_001099188.2
Danio rerio (zebrafish)	vps35	NP_001020688.2
	VPS35	XP_001108828.2
Gallus gallus (chicken)	VPS35	NP_001005842.1
Caenorhabditis elegans (roundworm)	vps-35	NP_495180.2
Saccharomyces cerevisiae (baker's yeast)	VPS35	NP_012381.1
Kluyveromyces lactis	KLLA0B07535g	XP_451864.1
Magnaporthe oryzae (rice blast fungus)	MGG_05089	XP_003712611.1
Bos taurus (cattle)	VPS35	NP_001039723.1
Drosophila melanogaster (fruit fly)	Vps35	NP_611651.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007683	XP_308188.4
Schizosaccharomyces pombe (fission yeast)	vps35	NP_588260.2
Oryza sativa (rice)	Os03g0801600	NP_001051593.1
Pan troglodytes (chimpanzee)	VPS35	XP_001161536.1
Mus musculus (house mouse)	Vps35	NP_075373.1
Neurospora crassa	NCU06284	XP_962546.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
Seiler C, Gebhart N, Zhang Y, Shinton SA, Li YS, Ross NL, Liu X, Li Q, Bilbee AN, Varshney GK, LaFave MC, Burgess SM, Balciuniene J, Balciunas D, Hardy RR, Kappes DJ, Wiest DL, Rhodes J
PloS one10(7)e0131908(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vps35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03765	NM_001025517.2 Latest version!	Danio rerio VPS35 retromer complex component (vps35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps35 ( NM_001025517.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001020688.2 Danio rerio VPS35 retromer complex component (vps35), mRNA.

Price & Availability↑

CloneID	ODa03765
Clone ID Related Accession (Same CDS sequence)	NM_001025517.2
Accession Version	NM_001025517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC139644.1 and BC117573.1. On Mar 25, 2009 this sequence version replaced NM_001025517.1. ##Evidence-Data-START## Transcript exon combination :: BC139644.1, BC117573.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGCGG GCAGCGCTCG TCATGAGGCG GAACAGCAGC AGTCACATGA TACGACACAT 
TCACAGGGGG GTCATCCAGT ATTTCCTCTA ACATACCTGA TAATCATGCC CACCACACAG 
CAGTCGCCGC AGGATGAGCA GGAGAAACTG CTGGATGAAG CTGTGCAGGC AGTCAAGGTT 
CAGTCATTTC AAATGAAGCG CTGTCTGGAC AAGAACAAGC TGATGGATGC ACTGAAACAT 
GCCTCAAATA TGCTGGGGGA GTTGAGGACG TCCATGCTCT CTCCTAAGAG CTATTATGAA 
CTCTATATGG CCATTTCAGA TGAGCTGCAT TACCTGGAGG TGTATTTGAC TGACGAGTTT 
GCCAAAGGTC GCAAAGTGGC TGATCTCTAT GAGCTTGTTC AGTATGCAGG GAACATCATC 
CCTCGACTGT ACTTGTTGAT AACTGTTGGA GTGGTGTACG TTCGCTCTTT CCCCCAGTCC 
CGCAAAGACA TACTGAAGGA CCTGGTGGAG ATGTGTCGCG GGGTGCAGCA TCCACTGCGT 
GGCCTTTTTC TCAGAAACTA CCTGCTGCAA TGCACGCGCA ACATCCTGCC AGATGACGGA 
GAACAGGCCG AGGAAGAGAT GACCGGAGAC ATTAATGACT CCATTGACTT TGTGCTGCTG 
AATTTTGCGG AGATGAATAA ACTGTGGGTG CGAATGCAGC ATCAGGGACA CAGCAGGGAC 
CGAGAAAAGA GAGAGAAAGA GAGGCAAGAG CTGCGCATCC TCGTTGGAAC TAATCTGGTC 
CGCCTGAGCC AGCTGGAAGG CGTCAATGTG GAAAAGTACA AACAGATCGT GCTGTCTGGG 
GTCTTGGAGC AGGTGGTGAA CTGTAGAGAC TCTCTAGCAC AGGAATACCT CATGGAGTGC 
ATCATACAGG TCTTTCCGGA TGAGTTCCAC CTGCAGACAT TGAACCCGTT CCTTCGTTCC 
TGTGCCGAGC TGCACCAAAA CGTGAATGTC AAGAACATCA TCATCGCTCT TATTGATAGA 
TTGGCCCTGT TTGCTCACAG AGAAGATGGA CCTGGAATCC CTGCTGAAAT CAAGTTATTT 
GATATTTTCT CACAGCAAGT GGCAACTGTC ATACAGTCTC GTCAGGACAT GCCCTCTGAA 
GATGTTGTTT CACTCCAAGT GTCTCTCATC AATCTGGCCA TGAAGTGCTA TCCTGACCGT 
GTGGATTATG TGGATAAAGT GTTGGAAAGC ACTGTGGAGA TTTTCAACAA GCTGAACCTG 
GAACACATTG CCACCAGCAG CGCGGTGTCT AAAGAACTGA CCAGGCTACT AAAGATCCCT 
GTGGACACGT ACAATAATAT CCTAACTGTG CTCCAGCTCA AGCATTTCCC TCCGCTCTTC 
GAGTACTTCG ATTATGAGTC ACGCAAAAGC ATGAGCTGCT ACGTGCTGAG CAACATTCTG 
GACTACAACA CCATAATCGT GGCCCAAGAT CAGGTCGATG CGATCCTGAA CCTGGTGTCT 
ACGCTGATCC AGGACCAGCC TGATCAGCCT GCTGAGGATC CGGACCCAGA GGACTTTGCA 
GAGGAACAGA GCCTAGTGGG CCGCTTCATT CATCTGCTGC ATTCAGACGA CCCTGACCAG 
CAGTATCTGA TTTTAAACAC TGCCAGGAAA CACTTTGGCG CTGGAGGGAA CCAGAGGATT 
CGCCACACTC TTCCCCCGCT GGTGTTCGCT GCGTATCAAC TGGCCTTTCG TTACAAAGAG 
AACTCCTCCT CAGATGATAA ATGGGAAAAG AAATGTCAGA AGATTTTCTC ATTCGCCCAT 
CAGACCATCA GTGCTCTGAT CAAAGCTGAG CTGGCCGAGT TACCCTTAAG GCTCTTCCTC 
CAGGGCGCTC TGGCGGCAGG CGAGATCGGC TTTGAAAACC ATGAAACTGT GGCCTACGAG 
TTCATGTCTC AGGCATTTTC TCTGTATGAA GATGAGATAA GCGATTCCAA GGCACAGCTG 
GCCGCCATTA CGCTGATCAT TGGCACGTTT GAACGCACAC GGTGCTTCAG CGAAGAGAAC 
CATGAGCCCC TTCGTACCCA GTGTGCTCTG GCGGCCTCCA AACTGCTGAA GAAGCCCGAT 
CAGTGCCGTG CAGTCAGCAT CTGTGCCCAC CTTTTCTGGT CAGGACGCAA CACTGACAAA 
GGAGGAGATG AGATTCGTGA TGGACGTAGG GTGATGGAAT GCTTGAAGAA GGCTCTGAAG 
ATTGCCAACC AGTGCATGGA CCCGTCCTTG CAAGTCCAGC TCTTCATCGA GATCCTCAAC 
AGATACATCT GCTTCTATGA GCGAGAGAAT GATGCGGTGA CGGTCCAGGT CCTGAATCAG 
CTGATCCAGA AGATCCGTGA AGATCTCCCG AACCTGGAGG CCAGCGAGGA GACGGAGCAG 
ATCAACAAAC ACTTCCACAA CACACTGGAG CACCTGCGTC TGCAGAGAGA GTCGCCCGAG 
TCTGAGGGAC CTGCTTACGA AGGGCTGGTG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020688.2
CDS	212..2707
Translation	MCAGSARHEAEQQQSHDTTHSQGGHPVFPLTYLIIMPTTQQSPQDEQEKLLDEAVQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVRSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDDGEQAEEEMTGDINDSIDFVLLNFAEMNKLWVRMQHQGHSRDREKREKERQELRILVGTNLVRLSQLEGVNVEKYKQIVLSGVLEQVVNCRDSLAQEYLMECIIQVFPDEFHLQTLNPFLRSCAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLESTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLQLKHFPPLFEYFDYESRKSMSCYVLSNILDYNTIIVAQDQVDAILNLVSTLIQDQPDQPAEDPDPEDFAEEQSLVGRFIHLLHSDDPDQQYLILNTARKHFGAGGNQRIRHTLPPLVFAAYQLAFRYKENSSSDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERTRCFSEENHEPLRTQCALAASKLLKKPDQCRAVSICAHLFWSGRNTDKGGDEIRDGRRVMECLKKALKIANQCMDPSLQVQLFIEILNRYICFYERENDAVTVQVLNQLIQKIREDLPNLEASEETEQINKHFHNTLEHLRLQRESPESEGPAYEGLVL

Target ORF information:

RefSeq Version	NM_001025517.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio VPS35 retromer complex component (vps35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025517.2

ORF Insert Sequence:

ATGTGTGCGG GCAGCGCTCG TCATGAGGCG GAACAGCAGC AGTCACATGA TACGACACAT 
TCACAGGGGG GTCATCCAGT ATTTCCTCTA ACATACCTGA TAATCATGCC CACCACACAG 
CAGTCGCCGC AGGATGAGCA GGAGAAACTG CTGGATGAAG CTGTGCAGGC AGTCAAGGTT 
CAGTCATTTC AAATGAAGCG CTGTCTGGAC AAGAACAAGC TGATGGATGC ACTGAAACAT 
GCCTCAAATA TGCTGGGGGA GTTGAGGACG TCCATGCTCT CTCCTAAGAG CTATTATGAA 
CTCTATATGG CCATTTCAGA TGAGCTGCAT TACCTGGAGG TGTATTTGAC TGACGAGTTT 
GCCAAAGGTC GCAAAGTGGC TGATCTCTAT GAGCTTGTTC AGTATGCAGG GAACATCATC 
CCTCGACTGT ACTTGTTGAT AACTGTTGGA GTGGTGTACG TTCGCTCTTT CCCCCAGTCC 
CGCAAAGACA TACTGAAGGA CCTGGTGGAG ATGTGTCGCG GGGTGCAGCA TCCACTGCGT 
GGCCTTTTTC TCAGAAACTA CCTGCTGCAA TGCACGCGCA ACATCCTGCC AGATGACGGA 
GAACAGGCCG AGGAAGAGAT GACCGGAGAC ATTAATGACT CCATTGACTT TGTGCTGCTG 
AATTTTGCGG AGATGAATAA ACTGTGGGTG CGAATGCAGC ATCAGGGACA CAGCAGGGAC 
CGAGAAAAGA GAGAGAAAGA GAGGCAAGAG CTGCGCATCC TCGTTGGAAC TAATCTGGTC 
CGCCTGAGCC AGCTGGAAGG CGTCAATGTG GAAAAGTACA AACAGATCGT GCTGTCTGGG 
GTCTTGGAGC AGGTGGTGAA CTGTAGAGAC TCTCTAGCAC AGGAATACCT CATGGAGTGC 
ATCATACAGG TCTTTCCGGA TGAGTTCCAC CTGCAGACAT TGAACCCGTT CCTTCGTTCC 
TGTGCCGAGC TGCACCAAAA CGTGAATGTC AAGAACATCA TCATCGCTCT TATTGATAGA 
TTGGCCCTGT TTGCTCACAG AGAAGATGGA CCTGGAATCC CTGCTGAAAT CAAGTTATTT 
GATATTTTCT CACAGCAAGT GGCAACTGTC ATACAGTCTC GTCAGGACAT GCCCTCTGAA 
GATGTTGTTT CACTCCAAGT GTCTCTCATC AATCTGGCCA TGAAGTGCTA TCCTGACCGT 
GTGGATTATG TGGATAAAGT GTTGGAAAGC ACTGTGGAGA TTTTCAACAA GCTGAACCTG 
GAACACATTG CCACCAGCAG CGCGGTGTCT AAAGAACTGA CCAGGCTACT AAAGATCCCT 
GTGGACACGT ACAATAATAT CCTAACTGTG CTCCAGCTCA AGCATTTCCC TCCGCTCTTC 
GAGTACTTCG ATTATGAGTC ACGCAAAAGC ATGAGCTGCT ACGTGCTGAG CAACATTCTG 
GACTACAACA CCATAATCGT GGCCCAAGAT CAGGTCGATG CGATCCTGAA CCTGGTGTCT 
ACGCTGATCC AGGACCAGCC TGATCAGCCT GCTGAGGATC CGGACCCAGA GGACTTTGCA 
GAGGAACAGA GCCTAGTGGG CCGCTTCATT CATCTGCTGC ATTCAGACGA CCCTGACCAG 
CAGTATCTGA TTTTAAACAC TGCCAGGAAA CACTTTGGCG CTGGAGGGAA CCAGAGGATT 
CGCCACACTC TTCCCCCGCT GGTGTTCGCT GCGTATCAAC TGGCCTTTCG TTACAAAGAG 
AACTCCTCCT CAGATGATAA ATGGGAAAAG AAATGTCAGA AGATTTTCTC ATTCGCCCAT 
CAGACCATCA GTGCTCTGAT CAAAGCTGAG CTGGCCGAGT TACCCTTAAG GCTCTTCCTC 
CAGGGCGCTC TGGCGGCAGG CGAGATCGGC TTTGAAAACC ATGAAACTGT GGCCTACGAG 
TTCATGTCTC AGGCATTTTC TCTGTATGAA GATGAGATAA GCGATTCCAA GGCACAGCTG 
GCCGCCATTA CGCTGATCAT TGGCACGTTT GAACGCACAC GGTGCTTCAG CGAAGAGAAC 
CATGAGCCCC TTCGTACCCA GTGTGCTCTG GCGGCCTCCA AACTGCTGAA GAAGCCCGAT 
CAGTGCCGTG CAGTCAGCAT CTGTGCCCAC CTTTTCTGGT CAGGACGCAA CACTGACAAA 
GGAGGAGATG AGATTCGTGA TGGACGTAGG GTGATGGAAT GCTTGAAGAA GGCTCTGAAG 
ATTGCCAACC AGTGCATGGA CCCGTCCTTG CAAGTCCAGC TCTTCATCGA GATCCTCAAC 
AGATACATCT GCTTCTATGA GCGAGAGAAT GATGCGGTGA CGGTCCAGGT CCTGAATCAG 
CTGATCCAGA AGATCCGTGA AGATCTCCCG AACCTGGAGG CCAGCGAGGA GACGGAGCAG 
ATCAACAAAC ACTTCCACAA CACACTGGAG CACCTGCGTC TGCAGAGAGA GTCGCCCGAG 
TCTGAGGGAC CTGCTTACGA AGGGCTGGTG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
PloS one10(7)e0131908(2015)
Seiler C,Gebhart N,Zhang Y,Shinton SA,Li YS,Ross NL,Liu X,Li Q,Bilbee AN,Varshney GK,LaFave MC,Burgess SM,Balciuniene J,Balciunas D,Hardy RR,Kappes DJ,Wiest DL,Rhodes J

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vps35 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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