flot1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	flot1a
Entrez Gene ID	561345
Full Name	flotillin 1a
Synonyms	flot1,reggie2,si:dz44o19.1,wu:fj45g11
General protein information	Preferred Names flotillin 1a Names flotillin 1a flotillin 1 reggie-2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005159441.4	XP_005159498.1	flotillin 1a isoform X1
XM_005159441.3	XP_005159498.1	flotillin 1a isoform X1
NM_130913.1	NP_570988.1	flotillin 1a

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	flot1b	NP_958864.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007494	XP_308381.4
Homo sapiens (human)	FLOT1	NP_005794.1
	FLOT1	NP_001098638.1
Drosophila melanogaster (fruit fly)	Flo-1	NP_725476.1
Xenopus tropicalis (tropical clawed frog)	flot1	NP_001039106.1
Canis lupus familiaris (dog)	FLOT1	XP_848778.1
Mus musculus (house mouse)	Flot1	NP_032053.1
Rattus norvegicus (Norway rat)	Flot1	NP_073192.2
Danio rerio (zebrafish)	flot1a	NP_570988.1
Pan troglodytes (chimpanzee)	FLOT1	NP_001035840.1
Bos taurus (cattle)	FLOT1	NP_001070355.1

Gene Ontology
Bibliography

Related articles in PubMed

Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion.
Morris EAR, Bodin S, Delaval B, Comunale F, Georget V, Costa ML, Lutfalla G, Gauthier-Rouvi?re C
Biology of the cell109(5)210-221(2017 May)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results provide the first in vivo evidence that Flotillins regulate E-cadherin-mediated cell-cell junctions to allow epiboly progression.
Title: Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion.

Analysis of reggie mRNA and protein expression patterns during early zebrafish development revealed that re-1a mRNA is present at high levels in expression domains that overlap with the combined expression pattern of both re-2 genes except at the somites.
Title: Restricted expression of reggie genes and proteins during early zebrafish development.

The following flot1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the flot1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01701	NM_130913.1 Latest version!	Danio rerio flotillin 1a (flot1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa23888	XM_005159441.4 Latest version!	Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa23888	XM_005159441.3	Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

flot1a ( NM_130913.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_570988.1 Danio rerio flotillin 1a (flot1a), mRNA.

Price & Availability↑

CloneID	ODa01701
Clone ID Related Accession (Same CDS sequence)	NM_130913.1
Accession Version	NM_130913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	flotillin 1a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX927188.11. On May 17, 2009 this sequence version replaced XM_684752.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGTGCATT 
GAAATCAAAA CCTCCAGGGA AGAAAGCTGT TCTTTATATT TTGTAATGCA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570988.1
CDS	66..1382
Translation	MFYTCGPNEAMVVSGFCRSPPVMISGGRVFVFPCVQQIQRISLNTLTLNVKSDKVYTRHGVPISVTGIAQMKIQGQNKQMLAAACQMFLGKSDSEIAHIALETLEGHQRAIIAHLTVEEIYKDRKKFSEQVFKVASSDLVNMGISVVSYTLKDVHDDQDYLHSLGKARTAQVQKDARIGEAKNKRDAVIREAHAMQEKVSAQYMNEIEMAKAQRDYELKKAIYDIEVFTKKAESEMAYQLQVAKTKQRIEEEKMQVLVVERSQQITLQEQEISRKEKELEAKVKKPAEAERYRLEKLAEAERLQLIMEAEAEAESIRVRGEAEAYAVEAKGRAEAEQMAKKAEAFQHYKEGAMVDMLLEKLPMMADEISKPLSATNKVTMVSSGGSEIGAAKLTGEVLDIMTKLPETIEKLTGVNISQCIEIKTSREESCSLYFVMQS

Target ORF information:

RefSeq Version	NM_130913.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio flotillin 1a (flot1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130913.1

ORF Insert Sequence:

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGTGCATT 
GAAATCAAAA CCTCCAGGGA AGAAAGCTGT TCTTTATATT TTGTAATGCA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flot1a ( XM_005159441.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159498.1 Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23888
Clone ID Related Accession (Same CDS sequence)	XM_005159441.4 , XM_005159441.3
Accession Version	XM_005159441.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	flotillin 1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159441.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGGTGGCC 
CGAACCGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159498.1
CDS	161..1432
Translation	MFYTCGPNEAMVVSGFCRSPPVMISGGRVFVFPCVQQIQRISLNTLTLNVKSDKVYTRHGVPISVTGIAQMKIQGQNKQMLAAACQMFLGKSDSEIAHIALETLEGHQRAIIAHLTVEEIYKDRKKFSEQVFKVASSDLVNMGISVVSYTLKDVHDDQDYLHSLGKARTAQVQKDARIGEAKNKRDAVIREAHAMQEKVSAQYMNEIEMAKAQRDYELKKAIYDIEVFTKKAESEMAYQLQVAKTKQRIEEEKMQVLVVERSQQITLQEQEISRKEKELEAKVKKPAEAERYRLEKLAEAERLQLIMEAEAEAESIRVRGEAEAYAVEAKGRAEAEQMAKKAEAFQHYKEGAMVDMLLEKLPMMADEISKPLSATNKVTMVSSGGSEIGAAKLTGEVLDIMTKLPETIEKLTGVNISQVARTG

Target ORF information:

RefSeq Version	XM_005159441.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159441.4

ORF Insert Sequence:

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGGTGGCC 
CGAACCGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flot1a ( XM_005159441.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159498.1 Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23888
Clone ID Related Accession (Same CDS sequence)	XM_005159441.4 , XM_005159441.3
Accession Version	XM_005159441.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	flotillin 1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGGTGGCC 
CGAACCGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159498.1
CDS	152..1423
Translation	MFYTCGPNEAMVVSGFCRSPPVMISGGRVFVFPCVQQIQRISLNTLTLNVKSDKVYTRHGVPISVTGIAQMKIQGQNKQMLAAACQMFLGKSDSEIAHIALETLEGHQRAIIAHLTVEEIYKDRKKFSEQVFKVASSDLVNMGISVVSYTLKDVHDDQDYLHSLGKARTAQVQKDARIGEAKNKRDAVIREAHAMQEKVSAQYMNEIEMAKAQRDYELKKAIYDIEVFTKKAESEMAYQLQVAKTKQRIEEEKMQVLVVERSQQITLQEQEISRKEKELEAKVKKPAEAERYRLEKLAEAERLQLIMEAEAEAESIRVRGEAEAYAVEAKGRAEAEQMAKKAEAFQHYKEGAMVDMLLEKLPMMADEISKPLSATNKVTMVSSGGSEIGAAKLTGEVLDIMTKLPETIEKLTGVNISQVARTG

Target ORF information:

RefSeq Version	XM_005159441.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio flotillin 1a (flot1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159441.3

ORF Insert Sequence:

ATGTTTTATA CCTGCGGTCC CAATGAGGCA ATGGTGGTGT CAGGTTTCTG CCGTTCTCCA 
CCAGTCATGA TCTCTGGTGG GAGAGTGTTT GTTTTTCCAT GCGTCCAGCA GATTCAAAGG 
ATCTCTCTGA ATACTCTGAC GCTGAATGTA AAAAGCGACA AAGTGTACAC ACGTCATGGA 
GTGCCCATCT CCGTCACTGG AATCGCACAG ATGAAGATTC AAGGACAGAA TAAGCAGATG 
TTGGCTGCAG CGTGTCAGAT GTTTTTGGGC AAGTCAGACA GTGAAATTGC CCACATCGCC 
TTGGAAACGC TGGAGGGCCA CCAGAGAGCC ATCATTGCTC ACCTCACTGT GGAGGAGATC 
TACAAAGACC GCAAGAAGTT CTCCGAGCAG GTGTTCAAGG TGGCTTCATC TGACCTGGTG 
AACATGGGCA TCAGTGTGGT CAGCTACACA CTCAAAGACG TTCACGATGA CCAGGATTAC 
CTGCACTCTT TGGGAAAGGC CCGTACTGCA CAAGTGCAGA AGGATGCTCG TATCGGTGAA 
GCTAAGAACA AAAGAGATGC AGTAATCAGG GAGGCTCATG CTATGCAGGA GAAGGTGTCT 
GCTCAGTACA TGAATGAGAT CGAGATGGCT AAAGCGCAGA GAGACTACGA GCTGAAGAAG 
GCCATCTACG ACATTGAGGT CTTCACCAAA AAAGCGGAAT CTGAAATGGC CTATCAGCTC 
CAGGTGGCGA AGACAAAGCA GCGGATCGAG GAGGAGAAGA TGCAGGTGCT GGTGGTGGAG 
CGATCGCAAC AAATCACGCT GCAGGAGCAG GAGATCTCAC GCAAAGAGAA GGAGCTGGAG 
GCCAAAGTCA AGAAGCCCGC CGAGGCCGAG CGATACCGCC TGGAGAAACT GGCTGAAGCC 
GAGCGCCTTC AGCTCATCAT GGAGGCAGAA GCAGAGGCTG AATCCATCAG AGTGAGAGGA 
GAGGCAGAAG CGTACGCTGT GGAGGCTAAA GGTCGTGCTG AAGCAGAGCA GATGGCAAAG 
AAAGCTGAAG CCTTCCAGCA CTACAAAGAA GGTGCAATGG TGGACATGCT GCTGGAGAAA 
CTTCCAATGA TGGCTGATGA GATCAGTAAG CCTCTGTCAG CTACAAATAA GGTGACTATG 
GTCTCCAGCG GAGGTTCAGA GATTGGTGCT GCTAAACTGA CAGGCGAAGT CCTCGACATC 
ATGACCAAAC TGCCTGAAAC CATCGAGAAA CTGACCGGCG TTAACATCTC CCAGGTGGCC 
CGAACCGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Flotillins control zebrafish epiboly through their role in cadherin-mediated cell-cell adhesion.
Biology of the cell109(5)210-221(2017 May)
Morris EAR,Bodin S,Delaval B,Comunale F,Georget V,Costa ML,Lutfalla G,Gauthier-Rouvi?re C

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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flot1a cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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