wnk1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnk1b
Entrez Gene ID	561159
Full Name	WNK lysine deficient protein kinase 1b
Synonyms	fc14f06,si:ch211-240l19.1,wnk1,wu:fc14f06
General protein information	Preferred Names WNK lysine deficient protein kinase 1b Names serine/threonine-protein kinase WNK1 WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009300083.3	XP_009298358.1	serine/threonine-protein kinase WNK1 isoform X1
XM_009300085.3	XP_009298360.1	serine/threonine-protein kinase WNK1 isoform X2
XM_009300087.3	XP_009298362.1	serine/threonine-protein kinase WNK1 isoform X5
XM_009300088.3	XP_009298363.1	serine/threonine-protein kinase WNK1 isoform X7
XM_009300091.3	XP_009298366.1	serine/threonine-protein kinase WNK1 isoform X9
XM_009300092.3	XP_009298367.1	serine/threonine-protein kinase WNK1 isoform X10
XM_009300093.3	XP_009298368.1	serine/threonine-protein kinase WNK1 isoform X11
XM_009300094.3	XP_009298369.1	serine/threonine-protein kinase WNK1 isoform X14
XM_021471501.1	XP_021327176.1	serine/threonine-protein kinase WNK1 isoform X1
XM_021471510.1	XP_021327185.1	serine/threonine-protein kinase WNK1 isoform X9
XM_017355253.2	XP_017210742.2	serine/threonine-protein kinase WNK1 isoform X12
XM_021471513.1	XP_021327188.1	serine/threonine-protein kinase WNK1 isoform X13
XM_009300084.2	XP_009298359.1	serine/threonine-protein kinase WNK1 isoform X1
XM_009300091.2	XP_009298366.1	serine/threonine-protein kinase WNK1 isoform X9
XM_009300092.2	XP_009298367.1	serine/threonine-protein kinase WNK1 isoform X10
XM_009300094.2	XP_009298369.1	serine/threonine-protein kinase WNK1 isoform X14
XM_009300083.2	XP_009298358.1	serine/threonine-protein kinase WNK1 isoform X1
XM_009300088.2	XP_009298363.1	serine/threonine-protein kinase WNK1 isoform X6
XM_009300093.2	XP_009298368.1	serine/threonine-protein kinase WNK1 isoform X11
XM_021471509.1	XP_021327184.1	serine/threonine-protein kinase WNK1 isoform X17
XM_021475204.1	XP_021330879.1	serine/threonine-protein kinase WNK1 isoform X6
XM_009300085.2	XP_009298360.1	serine/threonine-protein kinase WNK1 isoform X2
XM_005164548.3	XP_005164605.1	serine/threonine-protein kinase WNK1 isoform X3
XM_009300086.2	XP_009298361.1	serine/threonine-protein kinase WNK1 isoform X4
XM_017355253.1	XP_017210742.1	serine/threonine-protein kinase WNK1 isoform X12
XM_021471506.1	XP_021327181.1	serine/threonine-protein kinase WNK1 isoform X6
XM_021471508.1	XP_021327183.1	serine/threonine-protein kinase WNK1 isoform X8
XM_021471515.1	XP_021327190.1	serine/threonine-protein kinase WNK1 isoform X15
XM_009300086.3	XP_009298361.1	serine/threonine-protein kinase WNK1 isoform X4
XM_009300089.3	XP_009298364.1	serine/threonine-protein kinase WNK1 isoform X8
XM_021475205.1	XP_021330880.1	serine/threonine-protein kinase WNK1 isoform X12
XM_021475206.1	XP_021330881.1	serine/threonine-protein kinase WNK1 isoform X13
XM_021475208.1	XP_021330883.1	serine/threonine-protein kinase WNK1 isoform X16
XM_009300089.2	XP_009298364.1	serine/threonine-protein kinase WNK1 isoform X7
XM_009300087.2	XP_009298362.1	serine/threonine-protein kinase WNK1 isoform X5
XM_021471502.1	XP_021327177.1	serine/threonine-protein kinase WNK1 isoform X2
XM_021471503.1	XP_021327178.1	serine/threonine-protein kinase WNK1 isoform X3
XM_021471505.1	XP_021327180.1	serine/threonine-protein kinase WNK1 isoform X5
XM_021471507.1	XP_021327182.1	serine/threonine-protein kinase WNK1 isoform X7
XM_021471511.1	XP_021327186.1	serine/threonine-protein kinase WNK1 isoform X10
XM_009300090.2	XP_009298365.1	serine/threonine-protein kinase WNK1 isoform X8
XM_017355254.1	XP_017210743.1	serine/threonine-protein kinase WNK1 isoform X13
XM_005164548.4	XP_005164605.1	serine/threonine-protein kinase WNK1 isoform X3
XM_021475207.1	XP_021330882.1	serine/threonine-protein kinase WNK1 isoform X15
XM_021471504.1	XP_021327179.1	serine/threonine-protein kinase WNK1 isoform X4
XM_021471512.1	XP_021327187.1	serine/threonine-protein kinase WNK1 isoform X11
XM_021471514.1	XP_021327189.1	serine/threonine-protein kinase WNK1 isoform X14
XM_021471516.1	XP_021327191.1	serine/threonine-protein kinase WNK1 isoform X16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WNK1	NP_001171977.1
Danio rerio (zebrafish)	wnk1b	XP_005164610.1
Gallus gallus (chicken)	WNK1	XP_004937984.1
	WNK1	XP_001095845.1
Xenopus tropicalis (tropical clawed frog)	wnk1	NP_001090703.1

Gene Ontology

The following wnk1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142113	XM_021475208.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa144496	XM_021471516.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142110	XM_021475204.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142111	XM_021475206.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142112	XM_021475207.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa144480	XM_021471501.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144481	XM_021471502.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144482	XM_021471503.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144483	XM_021471504.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144484	XM_021471505.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144485	XM_021471506.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144486	XM_021471507.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144487	XM_021471508.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144488	XM_021471509.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144489	XM_021471510.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144490	XM_021471511.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144491	XM_021471512.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144492	XM_017355253.2 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144493	XM_021471513.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa144494	XM_021471514.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa144495	XM_021471515.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa58533	XM_021475205.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa58533	XM_017355253.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa58534	XM_017355254.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa09175	XM_005164548.4 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43442	XM_009300084.2 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43442	XM_009300083.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43443	XM_009300085.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43444	XM_009300086.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43445	XM_009300087.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43446	XM_009300088.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43447	XM_009300089.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43448	XM_009300090.2 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43449	XM_009300091.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43450	XM_009300092.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43451	XM_009300093.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43452	XM_009300094.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa43449	XM_009300091.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43450	XM_009300092.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43452	XM_009300094.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa43442	XM_009300083.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43446	XM_009300088.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43451	XM_009300093.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43443	XM_009300085.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43444	XM_009300086.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43447	XM_009300089.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43445	XM_009300087.2	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa09175	XM_005164548.3	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnk1b ( XM_021475208.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330883.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

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CloneID	ODa142113
Clone ID Related Accession (Same CDS sequence)	XM_021475208.1
Accession Version	XM_021475208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTT GCGTGCCCTG CTTTTCTACC ACCTCTGCTC TGTCGTGCCC TCCCAAGCCA 
CCTTCCACCC CTCCTCCAGT CGCATCAGCC CCGCTCTCAC CCCGGGTGGG GAGGCCAGAT 
GGGGAGACGT TCGCAGGCCG ACTGTCCAAA GCACTGGAGA GCGTCCTGCC CCTTCACTCG 
GCCCCGCCCC GTCGTCGTGC CAGCCTCCCA GCAATCTTTG CCAGTCCTCC GCAATCAGTG 
AGTCAGCCGT ACGGCGGAGG AGGCTCAGGT CTGGTCGAGC CCCCACTCTT TTTCCCCACC 
ATCCCGGAAC GGCCCGTCTC ATTCTCCCCT CCACCATCTT GCCCACCTAA AGCTTTCAAC 
GTGCAGCGCC GCAAGAGCAC CTCCATCCTG GAGGCCCACA CGCGTCACTT CCAGCCCGCC 
TACCACCGGA GCTATGGTAG CAGCCTGCAC CCATTCTCTG GCGTGGAGGC ACTTGGTGCC 
ACAGTCGAAC CTTCTATGTT TATGATGCCC GGGATGCCGG CTCAAAGATT GGCGGATCCA 
CTTCACCTAC AGCCTCCAAC AGAATCACTA TATGGATATA AAGATGTAAG AGCAGAGCAA 
GAAGAAACAG TACGCAGGCT CAGCCTCAAC CAGGCTGCAT TAATGGACCA TCTTGAAGCC 
ATGGCCTACA GCGGCTACCC TATGACTGCG CATCAGCTAA GCCACATCAG CTTCCATCAG 
CAAATGCAAG CCGCTGCTGC TAGCTTAGCC TATGAGACTC AGTCTCAGCA ACCTGGGCCA 
GCCTACCTAC ACCCTCACCT ACAGAGCCAC ATGCGCTTGA CCCACAGCCC AATCCCCCAA 
CTACCTCCAA TGAGTGTCCC TAGCTCTGCT TCTGCACCGC AGCAAGCCAC AGTTGCAGAT 
CCAAGCTATC AGTCCCACTC GTTTCCCCAT TCTGCACCAC CTGTGTTGGC CCATGCATCA 
GAGCCGAACA CAGCCTTTGA ATTCCACATG CACAATCTCC AGGCAGACCC AGCCATGTTG 
GCATCTAGAC TCTACCGAGC CCGTCGGGGC TCCATGGACC TTAACCTGGA GGAAGCAGGT 
GGCAACCCGG GATTGTGTAG CCGACTCCAG CCAGTCACAG AAGAACTGTA TAGTTATGTG 
AGCCCAGAGC TCCCTCTTCC ACCAGGCAGC CTGCTTCTTC ATGGACCTAA AGAGCTCAGT 
CCTGAACCAT CAAGTGACTC CATGACCTCC TCAGATGCGG GGGAGTTTCA CTCACCTCCG 
CCACATCAAT TGTACCAAAC TCAAGAGTGT TCTGCTGCTC AATCCATCCC ACAAGCAGCT 
TACTACAAAA CAGATGGAGG GAGTAATGTC AGCGATGGAC ACCCACCCAG TCAACCTACG 
TTTCTGTTCG TTCCTCACTC TGAATCATCC ACAATTTCGT CTTCACACAT CAATGCCTGG 
GCCCGTCAAC CATCCCTTCC ACCGGACCTC ACATTCCACG AGCCTGCCTT GTCCCATCAG 
GGCGCTACTT TGGTTCAAGT CCCCATTCCT CAGTCTACAA GTGGCATGTC TCTGGCTTCC 
TGCAGCACCC CAGCCGCCTC TGCCTCCCAA CAGTATGGAG TTTACTACGT CCAAGCACTC 
CCTACGCAGG CTCCTGTTCA GCAGCCGGCC TCTCTAGGTC CCACTTCCCA ACCCTGTGCC 
TCGCTACCCC AACAACAAAC ACCCCAACAG CCTTCCAGCA CTGTAGCTCA GAGCATCTCC 
CAAACGCAAA TCACGCCAAC ACAGCTTTGC AGTCCGGCTC TCTGCGTTCC AACTATAGCA 
GTCACCCCGT TTCAGCAGCA ACCTGTGCCT GAAGCGCTGA CGTCCCTGAA AACTGCGCCA 
GCCACCTCCC CTTCAATGCT GCCGCCCCAT GTACCCCTAT TGGTAACAAC ACCTGTGCCA 
ACCATCCATG TGGCCACAGT CCCTTCCCCC ACCGAACATC CGTGTTTGCC AGCAGACGCC 
TTGGCGCAGA CGTCTCACAG CGCATCGCAG ACTTTGACAA CAGAGCTGTC ACAACTCCAA 
ACTCCTGCTG TGGAGAGCAG TCATTCTGAT GTAGCTTCTG GGCTCAGTGA TGGCAATGAG 
GGCGTAACAG GTGGTAGACA TGAGGGTCGT CCTGCCAAAA GACACCAGCG CAGATCGGTG 
CGCAGCCGTT CACGCAATGA AAAGACCTCG AAGGTCAAGC TCAGTGTGCT TAATATCTCC 
AGCATGGGTG ACAGAGTTGC AGAGTGTCAA TTAGAAACAC ACAACAGGAA AATGGTGACT 
TTCAAGTTCG ATCTTGATGG TGATAACCCA GAGGAAATTG CACAGATAAT GGTACAAAGT 
GAATTCATTC TTGAAAGCGA GCGTGAATCG TTCATCGATC GGATCCGAGA AGTGATCGAA 
ACAGCTGATG AGAAGGGGGT GGAGAGAGAG CGCAGTCAGA TGGCCAGTGA TCATGAGCAG 
CTTATGCCCA CAACATCTGC GCCTCTTGAA CATGGTAGAA CTTACAATCT CAGTGTACCA 
CCTAGTTCAG CTGCACAGGT GGTTCATTCT GCAGGCCGCA GGTTTATCGT GAGTCCAGTG 
CCTGAATCCA GACTGAAGGA TCAGTTCTTT AGCCCATCCC AACCTACAAT CCCACTTCAT 
GAAGAACCTG CCCCTGTGCC AGTTGCATTG CCTGCCTTGG GGCTCTCTGT GTCTGCGTCT 
GCTGTCAGCC TGCAGCAGGC GTTCACTGAG ATGCGTCAAG CCCACTTTGA CCCAGGTCCC 
AGCACTGCAC CCCCTATGCT CCACAACTCC ATGCCCCCTC TGCTTCCTGC CACAACACCC 
TTGCCCTCTA GTGCCACCCC AGTGGTCTCT CCCCCAGTGT CTTCAGAAGT GACCTCTTCG 
CCATCTCTAC CTTCTATAAA CCCTCCAGTG TCATCATCTC CTCCTCCAAC CGTCCCGGTC 
AATTCCGTAA CCTCCCCTCC AGCACAACTC ACCCTGCCTC AAAGTCAAGT TGCGCCTGCC 
GTCATCAGTG TTATTCCCAC CCCTTCCACT CTGCCAATGC CAGCGGTGCC AACTGCCACA 
TCCCTTCCAC AAACTTCAGT CACCGTACCT TCCAGCACAC CAATCGCTCC AATGGGATCA 
GTGTCCGGAG GCAGTGGAGA ACAACCATCC ATCCCTGCTA CTTCTTCGCC CACTAGCGCT 
ACAATCCAAA CGCCACAGCT GGCACCATCC ACCATCCCCA CCACCACGGC TCAGTCACAA 
GTGCCCCCTC TCCAGCCGGT GACCACAACC ATGCCAGTCG TGCAGCCCAC ACCAGTGCAC 
AGCCAACCAC AGACCACCAC CGTGCCCAAT CAGAGCCATG CGCATAGTGT TGAATGCGAC 
AGTGACCAGC AGGGCAAGGT TGATGACATC CAGGCTCTAG ATAAGAAGCT GCGCTCGCTC 
TTTATGGACT TGGGCTCCGG TCCCCCATCT GCCCAGTCTG ATGTCACATC CGACCCGACG 
GCGACTCCAA GCGTTCCTGG CACCTCGTCT CCAACCACAT GTGCCACCCC CAGCGGGACT 
CCACTCCCTC CCTCTAGTTT ACCCCTGAAC TCCTCCACTC AGTCTGCGGG GTCTCCCATG 
ACCTCCATGG GCAATGCCTC CACCCCGGTC GGCTACAGTC AGACCACTCC ATCCAAAGCT 
CCCTTAACAC GATTACCGGT GTTGCCTGTC GGCCCAGATC AGGCTGGCAC GCCGCCCACA 
GAGCACCTTC CACCCTTCCC AGGACCTGGT TTAATTCAGT CCCAGCAGCC CCTGGAGGAT 
CTGGATGCCC AGCTGAGACG GGCACTGAGT CCGGAGACCG TCCCAATCAG CAGTGTCACG 
CACCAGTCAT CTCTAACCGG GATGCCACCT GGAGGACAGC CAGTTCCGTT TTCTCTGGAT 
GATGAGCAAG CAGCCGCACC TGCAGCTGGA GGATACAAAC TGGGGCGATT CCAGGTGTCG 
GTGGCCACTG ATGAGAACAC CACACAGACT CCAACCAGCT CATCCTCTTC CACATCATCT 
TCCTCCACCA CCTCCTCTTC CTCCTCCTCC TGTAGTCTGT CCAGCCCTGA AAACACACTG 
CACAGAACCT CCTCTCCCCT CATAGACACA GGCGCAGATG TGGTCGATGG TCTCCCTGCT 
CTCCCCAACC AGACAACGAC CCAGCCTCAG CCCACCACCA TCGGCCGCTT CCAGGTCACG 
ACCAAAACAG ACACGCAGGT GGGACGCTTC TCTGTTAGCA AAGCACAGGA CGAGGTGACT 
ACATCCACCT CACAACCTCA GCCCACACCT CAAACCCATT CTGCTGTGGG CAACGGTCCA 
GCACAGAGTC CCGGGAGCTT AAACAACTCC ATCAGCTCCT ATTTCAGCAG CGATAATGAC 
TCGGAGTTTG AAGACGAGGA CTTTAAGAGA GAAGTCAGCA GGCTGAGAGA CAAGCACATG 
ATAGAGAGTC AGGCTCTATA TTCTCGTCAG AAAGCAGAGA TTGAGGCTCT GTTTGCCCGT 
TTGGGGAAAG TTCCACCTGC TATGGTTAAC CCACCTGTGG TGAACCCAGC CGGCCGCAGG 
AGACGCCCCA CCAAGAGCAA AAGCAGCAAA TCCAGCCGCG GGAGCTCGCA GGCTAGCAAG 
AGTCCCGTAC AGCCAGCAAC CAGCACTTTA TCAGCACAGA GCGCCCCTTC TGTGTACCCA 
GCCCAGCAGG CCTTCCTGGC CCCCGGGGGG ATGATGGATG GGGGGAGCAG CCCTCTGCTG 
CAGTCTTTCA AACCCTCGCC CTCCAATGAC AACCTGTGCT CCACTTTCAC CAGTGACGCC 
ACGCTCTCTG CGCCCAGTCT CTGTGTAACC TCGCAAGGCT GTGTAAAGTT CAGCTGTGGC 
TCGGAGAGAG TGACCTTCAA ACCAGGTGGC AGGAGGACGC GTTTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCCTGCA ACCAACTCTG TAGGACGAGC 
AGCACCAACA CGGTGGCAGG ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT 
CAGAGCTGCA AGGGCATGTT CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG 
GACGCCATGA ACCTATCACA GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT 
CAGGGCCACA GCTACGAAAT GGTTCCTCCA GTAAACATGG GCCGTAAGTA CTCAGCTCCG 
GGTCAGCTGT GCCCAAGCAT CGCTTCAACT CTGAGCGGCC ATCCACCAAT GCCCAACACT 
CCCGCTACCT CTCTAGGAGC TCGGAAGGGC TCCTTGTGTC CCACGCCGCA GTACGGCTAC 
CCCTCCGCAC CTTACAGCGC CCAGTGGGCT GGAGCGGCCA CGCACACCCA GGCCGGTCTG 
CTGGCCACCT CACAACCCCT GGGCCAATAT CCCCAGGTGC CCACCCCTTT ACAGACCTTC 
CACATCAGCA CTTTGCAAAA GTCAGTCAGC CACCCGGGCG GACCCAATCT GAAGAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330883.1
CDS	179..5701
Translation	MSVCVPCFSTTSALSCPPKPPSTPPPVASAPLSPRVGRPDGETFAGRLSKALESVLPLHSAPPRRRASLPAIFASPPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021475208.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475208.1

ORF Insert Sequence:

ATGTCTGTTT GCGTGCCCTG CTTTTCTACC ACCTCTGCTC TGTCGTGCCC TCCCAAGCCA 
CCTTCCACCC CTCCTCCAGT CGCATCAGCC CCGCTCTCAC CCCGGGTGGG GAGGCCAGAT 
GGGGAGACGT TCGCAGGCCG ACTGTCCAAA GCACTGGAGA GCGTCCTGCC CCTTCACTCG 
GCCCCGCCCC GTCGTCGTGC CAGCCTCCCA GCAATCTTTG CCAGTCCTCC GCAATCAGTG 
AGTCAGCCGT ACGGCGGAGG AGGCTCAGGT CTGGTCGAGC CCCCACTCTT TTTCCCCACC 
ATCCCGGAAC GGCCCGTCTC ATTCTCCCCT CCACCATCTT GCCCACCTAA AGCTTTCAAC 
GTGCAGCGCC GCAAGAGCAC CTCCATCCTG GAGGCCCACA CGCGTCACTT CCAGCCCGCC 
TACCACCGGA GCTATGGTAG CAGCCTGCAC CCATTCTCTG GCGTGGAGGC ACTTGGTGCC 
ACAGTCGAAC CTTCTATGTT TATGATGCCC GGGATGCCGG CTCAAAGATT GGCGGATCCA 
CTTCACCTAC AGCCTCCAAC AGAATCACTA TATGGATATA AAGATGTAAG AGCAGAGCAA 
GAAGAAACAG TACGCAGGCT CAGCCTCAAC CAGGCTGCAT TAATGGACCA TCTTGAAGCC 
ATGGCCTACA GCGGCTACCC TATGACTGCG CATCAGCTAA GCCACATCAG CTTCCATCAG 
CAAATGCAAG CCGCTGCTGC TAGCTTAGCC TATGAGACTC AGTCTCAGCA ACCTGGGCCA 
GCCTACCTAC ACCCTCACCT ACAGAGCCAC ATGCGCTTGA CCCACAGCCC AATCCCCCAA 
CTACCTCCAA TGAGTGTCCC TAGCTCTGCT TCTGCACCGC AGCAAGCCAC AGTTGCAGAT 
CCAAGCTATC AGTCCCACTC GTTTCCCCAT TCTGCACCAC CTGTGTTGGC CCATGCATCA 
GAGCCGAACA CAGCCTTTGA ATTCCACATG CACAATCTCC AGGCAGACCC AGCCATGTTG 
GCATCTAGAC TCTACCGAGC CCGTCGGGGC TCCATGGACC TTAACCTGGA GGAAGCAGGT 
GGCAACCCGG GATTGTGTAG CCGACTCCAG CCAGTCACAG AAGAACTGTA TAGTTATGTG 
AGCCCAGAGC TCCCTCTTCC ACCAGGCAGC CTGCTTCTTC ATGGACCTAA AGAGCTCAGT 
CCTGAACCAT CAAGTGACTC CATGACCTCC TCAGATGCGG GGGAGTTTCA CTCACCTCCG 
CCACATCAAT TGTACCAAAC TCAAGAGTGT TCTGCTGCTC AATCCATCCC ACAAGCAGCT 
TACTACAAAA CAGATGGAGG GAGTAATGTC AGCGATGGAC ACCCACCCAG TCAACCTACG 
TTTCTGTTCG TTCCTCACTC TGAATCATCC ACAATTTCGT CTTCACACAT CAATGCCTGG 
GCCCGTCAAC CATCCCTTCC ACCGGACCTC ACATTCCACG AGCCTGCCTT GTCCCATCAG 
GGCGCTACTT TGGTTCAAGT CCCCATTCCT CAGTCTACAA GTGGCATGTC TCTGGCTTCC 
TGCAGCACCC CAGCCGCCTC TGCCTCCCAA CAGTATGGAG TTTACTACGT CCAAGCACTC 
CCTACGCAGG CTCCTGTTCA GCAGCCGGCC TCTCTAGGTC CCACTTCCCA ACCCTGTGCC 
TCGCTACCCC AACAACAAAC ACCCCAACAG CCTTCCAGCA CTGTAGCTCA GAGCATCTCC 
CAAACGCAAA TCACGCCAAC ACAGCTTTGC AGTCCGGCTC TCTGCGTTCC AACTATAGCA 
GTCACCCCGT TTCAGCAGCA ACCTGTGCCT GAAGCGCTGA CGTCCCTGAA AACTGCGCCA 
GCCACCTCCC CTTCAATGCT GCCGCCCCAT GTACCCCTAT TGGTAACAAC ACCTGTGCCA 
ACCATCCATG TGGCCACAGT CCCTTCCCCC ACCGAACATC CGTGTTTGCC AGCAGACGCC 
TTGGCGCAGA CGTCTCACAG CGCATCGCAG ACTTTGACAA CAGAGCTGTC ACAACTCCAA 
ACTCCTGCTG TGGAGAGCAG TCATTCTGAT GTAGCTTCTG GGCTCAGTGA TGGCAATGAG 
GGCGTAACAG GTGGTAGACA TGAGGGTCGT CCTGCCAAAA GACACCAGCG CAGATCGGTG 
CGCAGCCGTT CACGCAATGA AAAGACCTCG AAGGTCAAGC TCAGTGTGCT TAATATCTCC 
AGCATGGGTG ACAGAGTTGC AGAGTGTCAA TTAGAAACAC ACAACAGGAA AATGGTGACT 
TTCAAGTTCG ATCTTGATGG TGATAACCCA GAGGAAATTG CACAGATAAT GGTACAAAGT 
GAATTCATTC TTGAAAGCGA GCGTGAATCG TTCATCGATC GGATCCGAGA AGTGATCGAA 
ACAGCTGATG AGAAGGGGGT GGAGAGAGAG CGCAGTCAGA TGGCCAGTGA TCATGAGCAG 
CTTATGCCCA CAACATCTGC GCCTCTTGAA CATGGTAGAA CTTACAATCT CAGTGTACCA 
CCTAGTTCAG CTGCACAGGT GGTTCATTCT GCAGGCCGCA GGTTTATCGT GAGTCCAGTG 
CCTGAATCCA GACTGAAGGA TCAGTTCTTT AGCCCATCCC AACCTACAAT CCCACTTCAT 
GAAGAACCTG CCCCTGTGCC AGTTGCATTG CCTGCCTTGG GGCTCTCTGT GTCTGCGTCT 
GCTGTCAGCC TGCAGCAGGC GTTCACTGAG ATGCGTCAAG CCCACTTTGA CCCAGGTCCC 
AGCACTGCAC CCCCTATGCT CCACAACTCC ATGCCCCCTC TGCTTCCTGC CACAACACCC 
TTGCCCTCTA GTGCCACCCC AGTGGTCTCT CCCCCAGTGT CTTCAGAAGT GACCTCTTCG 
CCATCTCTAC CTTCTATAAA CCCTCCAGTG TCATCATCTC CTCCTCCAAC CGTCCCGGTC 
AATTCCGTAA CCTCCCCTCC AGCACAACTC ACCCTGCCTC AAAGTCAAGT TGCGCCTGCC 
GTCATCAGTG TTATTCCCAC CCCTTCCACT CTGCCAATGC CAGCGGTGCC AACTGCCACA 
TCCCTTCCAC AAACTTCAGT CACCGTACCT TCCAGCACAC CAATCGCTCC AATGGGATCA 
GTGTCCGGAG GCAGTGGAGA ACAACCATCC ATCCCTGCTA CTTCTTCGCC CACTAGCGCT 
ACAATCCAAA CGCCACAGCT GGCACCATCC ACCATCCCCA CCACCACGGC TCAGTCACAA 
GTGCCCCCTC TCCAGCCGGT GACCACAACC ATGCCAGTCG TGCAGCCCAC ACCAGTGCAC 
AGCCAACCAC AGACCACCAC CGTGCCCAAT CAGAGCCATG CGCATAGTGT TGAATGCGAC 
AGTGACCAGC AGGGCAAGGT TGATGACATC CAGGCTCTAG ATAAGAAGCT GCGCTCGCTC 
TTTATGGACT TGGGCTCCGG TCCCCCATCT GCCCAGTCTG ATGTCACATC CGACCCGACG 
GCGACTCCAA GCGTTCCTGG CACCTCGTCT CCAACCACAT GTGCCACCCC CAGCGGGACT 
CCACTCCCTC CCTCTAGTTT ACCCCTGAAC TCCTCCACTC AGTCTGCGGG GTCTCCCATG 
ACCTCCATGG GCAATGCCTC CACCCCGGTC GGCTACAGTC AGACCACTCC ATCCAAAGCT 
CCCTTAACAC GATTACCGGT GTTGCCTGTC GGCCCAGATC AGGCTGGCAC GCCGCCCACA 
GAGCACCTTC CACCCTTCCC AGGACCTGGT TTAATTCAGT CCCAGCAGCC CCTGGAGGAT 
CTGGATGCCC AGCTGAGACG GGCACTGAGT CCGGAGACCG TCCCAATCAG CAGTGTCACG 
CACCAGTCAT CTCTAACCGG GATGCCACCT GGAGGACAGC CAGTTCCGTT TTCTCTGGAT 
GATGAGCAAG CAGCCGCACC TGCAGCTGGA GGATACAAAC TGGGGCGATT CCAGGTGTCG 
GTGGCCACTG ATGAGAACAC CACACAGACT CCAACCAGCT CATCCTCTTC CACATCATCT 
TCCTCCACCA CCTCCTCTTC CTCCTCCTCC TGTAGTCTGT CCAGCCCTGA AAACACACTG 
CACAGAACCT CCTCTCCCCT CATAGACACA GGCGCAGATG TGGTCGATGG TCTCCCTGCT 
CTCCCCAACC AGACAACGAC CCAGCCTCAG CCCACCACCA TCGGCCGCTT CCAGGTCACG 
ACCAAAACAG ACACGCAGGT GGGACGCTTC TCTGTTAGCA AAGCACAGGA CGAGGTGACT 
ACATCCACCT CACAACCTCA GCCCACACCT CAAACCCATT CTGCTGTGGG CAACGGTCCA 
GCACAGAGTC CCGGGAGCTT AAACAACTCC ATCAGCTCCT ATTTCAGCAG CGATAATGAC 
TCGGAGTTTG AAGACGAGGA CTTTAAGAGA GAAGTCAGCA GGCTGAGAGA CAAGCACATG 
ATAGAGAGTC AGGCTCTATA TTCTCGTCAG AAAGCAGAGA TTGAGGCTCT GTTTGCCCGT 
TTGGGGAAAG TTCCACCTGC TATGGTTAAC CCACCTGTGG TGAACCCAGC CGGCCGCAGG 
AGACGCCCCA CCAAGAGCAA AAGCAGCAAA TCCAGCCGCG GGAGCTCGCA GGCTAGCAAG 
AGTCCCGTAC AGCCAGCAAC CAGCACTTTA TCAGCACAGA GCGCCCCTTC TGTGTACCCA 
GCCCAGCAGG CCTTCCTGGC CCCCGGGGGG ATGATGGATG GGGGGAGCAG CCCTCTGCTG 
CAGTCTTTCA AACCCTCGCC CTCCAATGAC AACCTGTGCT CCACTTTCAC CAGTGACGCC 
ACGCTCTCTG CGCCCAGTCT CTGTGTAACC TCGCAAGGCT GTGTAAAGTT CAGCTGTGGC 
TCGGAGAGAG TGACCTTCAA ACCAGGTGGC AGGAGGACGC GTTTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCCTGCA ACCAACTCTG TAGGACGAGC 
AGCACCAACA CGGTGGCAGG ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT 
CAGAGCTGCA AGGGCATGTT CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG 
GACGCCATGA ACCTATCACA GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT 
CAGGGCCACA GCTACGAAAT GGTTCCTCCA GTAAACATGG GCCGTAAGTA CTCAGCTCCG 
GGTCAGCTGT GCCCAAGCAT CGCTTCAACT CTGAGCGGCC ATCCACCAAT GCCCAACACT 
CCCGCTACCT CTCTAGGAGC TCGGAAGGGC TCCTTGTGTC CCACGCCGCA GTACGGCTAC 
CCCTCCGCAC CTTACAGCGC CCAGTGGGCT GGAGCGGCCA CGCACACCCA GGCCGGTCTG 
CTGGCCACCT CACAACCCCT GGGCCAATAT CCCCAGGTGC CCACCCCTTT ACAGACCTTC 
CACATCAGCA CTTTGCAAAA GTCAGTCAGC CACCCGGGCG GACCCAATCT GAAGAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471516.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327191.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

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CloneID	ODa144496
Clone ID Related Accession (Same CDS sequence)	XM_021471516.1
Accession Version	XM_021471516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTTT GCGTGCCCTG CTTTTCTACC ACCTCTGCTC TGTCGTGCCC TCCCAAGCCA 
CCTTCCACCC CTCCTCCAGT CGCATCAGCC CCGCTCTCAC CTCGGGTGGG GAGGCCAGAT 
GGGGAGACGT TCGCAGGCCG ACTGTCCAAA GCACTGGAGA GCGTCCTGCC CCTTCACTCG 
GCCCCGCCCC GTCGTCGTGC CAGCCTCCCA GCAATCTTTG CCAGTCCTCC GCAATCAGTG 
AGTCAGCCGT ACGGCGGAGG AGGCTCAGGT CTGGTCGAGC CCCCACTCTT TTTCCCCACC 
ATCCCGGAAC GGCCCGTCTC ATTCTCCCCT CCACCATCTT GCCCACCTAA AGCTTTCAAC 
GTGCAGCGCC GCAAGAGCAC CTCCATCCTG GAGGCCCACA CGCGTCACTT CCAGCCCGCC 
TACCACCGGA GCTATGGTAG CAGCCTGCAC CCATTCTCTG GCGTGGAGGC ACTTGGTGCC 
ACAGTCGAAC CTTCTATGTT TATGATGCCC GGGATGCCGG CTCAAAGATT GGCGGATCCA 
CTTCACCTAC AGCCTCCAAC AGAATCACTA TATGGATATA AAGATGTAAG AGCAGAGCAA 
GAAGAAACAG TACGCAGGCT CAGCCTCAAC CAGGCTGCAT TAATGGACCA TCTTGAAGCC 
ATGGCCTACA GCGGCTACCC TATGACTGCG CATCAGCTAA GCCACATCAG CTTCCATCAG 
CAAATGCAAG CCGCTGCTGC TAGCTTAGCC TATGAGACTC AGTCTCAGCA ACCTGGGCCA 
GCCTACCTAC ACCCTCACCT ACAGAGCCAC ATGCGCTTGA CCCACAGCCC AATCCCCCAA 
CTACCTCCAA TGAGTGTCCC TAGCTCTGCT TCTGCACCGC AGCAAGCCAC AGTTGCAGAT 
CCAAGCTATC AGTCCCACTC GTTTCCCCAT TCTGCACCAC CTGTGTTGGC CCATGCATCA 
GAGCCGAACA CAGCCTTTGA ATTCCACATG CACAATCTCC AGGCAGACCC AGCCATGTTG 
GCATCTAGAC TCTACCGAGC CCGTCGGGGC TCCATGGACC TTAACCTGGA GGAAGCAGGT 
GGCAACCCGG GATTGTGTAG CCGACTCCAG CCAGTCACTG AAGAACTGTA TAGTTATGTG 
AGCCCAGAGC TGCCTCTTCC TCCAGGCAGC CTGCTTCTTC ATGGACCTAA AGAGCTCAGT 
CCTGAACCAT CAAGTGACTC CATGACCTCC TCAGATGCCG GGGAGTTTCA CTCACCTCCG 
CCACATCAAT TGTACCAAAC TCAAGAGTGT TCTGCTGCTC AATCCATCCC ACAAGCAGCT 
TACTACAAAA CAGATGGAGG GAGTAATGTC AGCGATGGAC ACCCACCCAG TCAACCTACG 
TTTCTGTTCG TTCCACACTC TGAATCATCC ACAATTTCGT CTTCACACAT CAATGCCTGG 
GCCCGTCAAC CATCCCTTCC ACCGGACCTC ACATTCCACG AGCCTGCCTT GTCCCATCAG 
GGCGCTACTT TGGTTCAAGT CCCCATTCCT CAGTCTACAA GTGGCATGTC TCTGGCTTCC 
TGCAGCACCC CAGCCGCCTC TGCCTCCCAA CAGTATGGAG TTTACTACGT CCAAGCACTC 
CCTACGCAGG CTCCTGTTCA GCAGCCGGCC TCTCTAGGTC CCACTTCCCA ACCCTGTGCC 
TCGCTACCCC AACAACAAAC ACCCCAACAG CCTTCCAGCA CTGTAGCTCA GAGCATCTCC 
CAAACGCAAA TCACGCCAAC ACAGCTTTGC AGTCCGGCTC TCTGCGTTCC AACTATAGCA 
GTCACCCCGT TTCAGCAGCA ACCTGTGCCT GAAGCGCTGA CGTCCCTGAA AACTGCGCCA 
GCCACCTCCC CTTCAATGCT GCCGCCCCAT GTACCCCTAT TGGTAACAAC ACCTGTGCCA 
ACCATCCATG TGGCCACAGT CCCTTCCCCC ACCGAACATC CGTGTTTGCC AGCAGACGCC 
TTGGCGCAGA CGTCTCACAG TGCATCGCAG ACTTTGACAA CAGAGCTGTC ACAACTCCAA 
ACTCCTGCTG TGGAGAGCAG TCATTCTGAT GTAGCTTCTG GGCTCAGTGA TGGCAATGAG 
GGCGTAACAG GTGGTAGACA TGAGGGTCGT CCTGCCAAAA GACACCAGCG CAGATCGGTG 
CGCAGCCGTT CACGCAATGA AAAGACCTCG AAGGTCAAGC TCAGTGTGCT TAATATCTCC 
AGCATGGGTG ACAGAGTTGC AGAGTGTCAA TTAGAAACAC ACAACAGGAA AATGGTGACT 
TTCAAGTTCG ATCTTGATGG TGATAACCCA GAGGAAATTG CACAGATAAT GGTACAAAGT 
GAATTCATTC TTGAAAGCGA GCGCGAATCG TTCATCGATC GGATCCGAGA AGTGATCGAA 
ACAGCTGATG AGAAGGGGGT GGAGAGAGAG CGCAGCCAGA TGGCCAGTGA TCATGAGCAG 
CTTATGCCCA CAACATCTGC GCCTCTTGAA CACGGTAGAA CTTACAATCT CAGTGTTCCA 
CCTAGTTCAG CTGCACAGGT GGTTCATTCT GCAGGCCGCA GGTTTATCGT GAGTCCAGTG 
CCTGAATCCA GACTGAAGGA TCAGTTCTTT AGCCCATCCC AACCTACAAT CCCACTTCAT 
GAAGAACCTG CCCCTGTGCC AGTTGCATTG CCTGCCTTGG GGCTCTCTGT GTCTGCGTCT 
GCTGTCAGCC TGCAGCAGGC GTTCACTGAG ATGCGTCAAG CCCACTTTGA CCCAGGTCCC 
AGCACTGCAC CCCCTATGCT CCACAACTCC ATGCCCCCTC TGCTTCCTGC CACAACACCC 
TTGCCCTCTA GTGCCACCCC GGTGGTCTCT CCCCCAGTGT CTTCAGAAGT GACCTCTTCG 
CCATCTCTAC CTTCTATAAA CCCTCCAGTG TCATCATCTC CTCCTCCAAC CGTCCCGGTC 
AATTCCGTAA CCTCCCCTCC AGCACAACTC ACCCTGCCTC AAAGTCAAGT TGCGCCTGCC 
GTCATCAGTG TTATTCCCAC CCCTTCCACT CTGCCAATGC CAGCGGTGCC AACTGCCACA 
TCCTTTCCAC AAACTTCAGT CACCGTACCT TCCAGCACAC CAATCGCTCC AATGGGATCA 
GTGTCCGGAG GCAGTGGAGA ACAACCATCC ATCCCTGCTA CTTCTTCGCC CACTAGCGCT 
ACAATCCAAA CGCCACAGCT GGCACCATCC ACCATCCCCA CCACCACGGC TCAGTCACAA 
GTGCCCCCTC TCCAGCCGGT GACCACAACC ATGCCAGTCG TGCAGCCCAC ACCAGTGCAC 
AGCCAACCAC AGACCACCAC CGTGCCCAAT CAGAGCCATG CGCATAGTGT TGAATGCGAC 
AGTGACCAGC AGGGCAAGGT TGATGACATC CAGGCTCTAG ATAAGAAGCT GCGCTCGCTC 
TTTATGGACT TGGGCTCCGG TCCCCCATCT GCCCAGTCTG ATGTCACATC CGACCCGACG 
GCGACTCCAA GCGTTCCTGG CACCTCGTCT CCAACCACAT GTGCCACCCC CAGCGGGACT 
CCACTCCCTC CCTCTAGTTT ACCCCTGAAC TCCTCCACTC AGTCTGCAGG GTCTCCCATG 
ACCTCCATGG GCAATGCCTC CACCCCGGTC GGCTACAGTC AGACCACTCC ATCCAAAGCT 
CCCTTAACAC GATTACCGGT GTTGCCTGTC GGCCCAGATC AGGCTGGCAC GCCGCCCACA 
GAGCACCTTC CACCCTTCCC AGGACCTGGT TTAATTCAGT CCCAGCAGCC CCTGGAGGAT 
CTGGATGCCC AGCTGAGACG GGCACTGAGT CCGGAGACCG TCCCAATCAG CAGTGTCACG 
CACCAGTCAT CTCTAACCGG GATGCCACCT GGAGGACAGC CAGTTCCGTT TTCTCTGGAT 
GATGAGCAAG CAGCCGCACC TGCAGCTGGA GGATACAAAC TGGGGCGATT CCAGGTGTCG 
GTGGCCACTG ATGAGAACAC CACACAGACT CCAACCAGCT CATCCTCTTC CACATCATCT 
TCCTCCACCA CCTCCTCTTC CTCCTCCTCC TGTAGTCTGT CCAGCCCTGA AAACACACTG 
CACAGAACCT CCTCTCCCCT CATAGACACA GGCGCAGATG TGGTCGATGG TCTCCCTGCT 
CTCCCCAACC AGACAACGAC CCAGCCTCAG CCCACCACCA TCGGCCGCTT CCAGGTCACG 
ACCAAAACAG ACACGCAGGT GGGACGCTTC TCTGTTAGCA AAGCACAGGA CGAGGTGACT 
ACATCCACCT TACAACCTCA GCCCACACCT CAAACCCATT CTGCTGTGGG CAACGGTCCA 
GCACAGAGTC CCGGGAGCTT AAACAACTCC ATCAGCTCCT ATTTCAGCAG CGATAATGAC 
TCGGAGTTTG AAGACGAGGA CTTCAAGAGA GAAGTCAGCA GGCTGAGAGA CAAGCACATG 
ATAGAGAGTC AGGCTCTATA TTCTCGTCAG AAAGCAGAGA TTGAGGCTCT GTTTGCCCGT 
TTGGGGAAAG TTCCACCTGC TATGGTTAAC CCACCTGTGG TGAACCCAGC CGGCCGCAGG 
AGACGCCCCA CCAAGAGCAA AAGCAGCAAA TCCAGCCGCG GGAGCTCGCA GGCTAGCAAG 
AGTCCCGTAC AGCCAGCAAC CAGCACTTTA TCAGCACAGA GCGCCCCCTC TGTGTACCCA 
GCCCAGCAGG CTTTCCTGGC CCCCGGGGGG ATGATGGATG GGGGGAGCAG CCCTCTGCTG 
CAGTCTTTCA AACCCTCGCC CTCCAATGAC AACCTGTGCT CCACTTTCAC CAGTGACGCC 
ACGCTCTCTG CGCCCAGTCT CTGTGTAACC TCGCAAGGCT GTGTAAAGTT CAGCTGTGGC 
TCGGAGAGAG TGACCTTCAA ACCAGGTGGC AGGAGGACGC GTTTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCCTGCA ACCAACTCTG TAGGACGAGC 
AGCACCAACA CGGTGGCAGG ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT 
CAGAGCTGCA AGGGCATGTT CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG 
GACGCCATGA ATCTATCACA GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT 
CAGGGCCACA GCTACGAAAT GGTTCCTCCA GTAAACATGG GCCGTAAGTA CTCAGCTCCG 
GGTCAGCTGT GCCCAAGCAT CGCTTCAACT CTGAGCGGCC ATCCACCAAT GCCCAACACT 
CCCGCTACCT CTCTAGGAGC TCGGAAGGGC TCCTTGTGTC CCACGCCGCA GTACGGCTAC 
CCCTCCGCAC CTTACAGCGC CCAGTGGGCT GGAGCGGCCA CGCACACCCA GGCCGGTCTG 
CTGGCCACCT CACAACCCCT GGGCCAATAT CCCCAGGTGC CCACCCCTTT ACAGACCTTC 
CACATCAGCA CTTTGCAAAA GTCAGTCAGC CACCCGGGTG GACCCAATCT AAAGAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327191.1
CDS	723..6245
Translation	MSVCVPCFSTTSALSCPPKPPSTPPPVASAPLSPRVGRPDGETFAGRLSKALESVLPLHSAPPRRRASLPAIFASPPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471516.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471516.1

ORF Insert Sequence:

ATGTCTGTTT GCGTGCCCTG CTTTTCTACC ACCTCTGCTC TGTCGTGCCC TCCCAAGCCA 
CCTTCCACCC CTCCTCCAGT CGCATCAGCC CCGCTCTCAC CTCGGGTGGG GAGGCCAGAT 
GGGGAGACGT TCGCAGGCCG ACTGTCCAAA GCACTGGAGA GCGTCCTGCC CCTTCACTCG 
GCCCCGCCCC GTCGTCGTGC CAGCCTCCCA GCAATCTTTG CCAGTCCTCC GCAATCAGTG 
AGTCAGCCGT ACGGCGGAGG AGGCTCAGGT CTGGTCGAGC CCCCACTCTT TTTCCCCACC 
ATCCCGGAAC GGCCCGTCTC ATTCTCCCCT CCACCATCTT GCCCACCTAA AGCTTTCAAC 
GTGCAGCGCC GCAAGAGCAC CTCCATCCTG GAGGCCCACA CGCGTCACTT CCAGCCCGCC 
TACCACCGGA GCTATGGTAG CAGCCTGCAC CCATTCTCTG GCGTGGAGGC ACTTGGTGCC 
ACAGTCGAAC CTTCTATGTT TATGATGCCC GGGATGCCGG CTCAAAGATT GGCGGATCCA 
CTTCACCTAC AGCCTCCAAC AGAATCACTA TATGGATATA AAGATGTAAG AGCAGAGCAA 
GAAGAAACAG TACGCAGGCT CAGCCTCAAC CAGGCTGCAT TAATGGACCA TCTTGAAGCC 
ATGGCCTACA GCGGCTACCC TATGACTGCG CATCAGCTAA GCCACATCAG CTTCCATCAG 
CAAATGCAAG CCGCTGCTGC TAGCTTAGCC TATGAGACTC AGTCTCAGCA ACCTGGGCCA 
GCCTACCTAC ACCCTCACCT ACAGAGCCAC ATGCGCTTGA CCCACAGCCC AATCCCCCAA 
CTACCTCCAA TGAGTGTCCC TAGCTCTGCT TCTGCACCGC AGCAAGCCAC AGTTGCAGAT 
CCAAGCTATC AGTCCCACTC GTTTCCCCAT TCTGCACCAC CTGTGTTGGC CCATGCATCA 
GAGCCGAACA CAGCCTTTGA ATTCCACATG CACAATCTCC AGGCAGACCC AGCCATGTTG 
GCATCTAGAC TCTACCGAGC CCGTCGGGGC TCCATGGACC TTAACCTGGA GGAAGCAGGT 
GGCAACCCGG GATTGTGTAG CCGACTCCAG CCAGTCACTG AAGAACTGTA TAGTTATGTG 
AGCCCAGAGC TGCCTCTTCC TCCAGGCAGC CTGCTTCTTC ATGGACCTAA AGAGCTCAGT 
CCTGAACCAT CAAGTGACTC CATGACCTCC TCAGATGCCG GGGAGTTTCA CTCACCTCCG 
CCACATCAAT TGTACCAAAC TCAAGAGTGT TCTGCTGCTC AATCCATCCC ACAAGCAGCT 
TACTACAAAA CAGATGGAGG GAGTAATGTC AGCGATGGAC ACCCACCCAG TCAACCTACG 
TTTCTGTTCG TTCCACACTC TGAATCATCC ACAATTTCGT CTTCACACAT CAATGCCTGG 
GCCCGTCAAC CATCCCTTCC ACCGGACCTC ACATTCCACG AGCCTGCCTT GTCCCATCAG 
GGCGCTACTT TGGTTCAAGT CCCCATTCCT CAGTCTACAA GTGGCATGTC TCTGGCTTCC 
TGCAGCACCC CAGCCGCCTC TGCCTCCCAA CAGTATGGAG TTTACTACGT CCAAGCACTC 
CCTACGCAGG CTCCTGTTCA GCAGCCGGCC TCTCTAGGTC CCACTTCCCA ACCCTGTGCC 
TCGCTACCCC AACAACAAAC ACCCCAACAG CCTTCCAGCA CTGTAGCTCA GAGCATCTCC 
CAAACGCAAA TCACGCCAAC ACAGCTTTGC AGTCCGGCTC TCTGCGTTCC AACTATAGCA 
GTCACCCCGT TTCAGCAGCA ACCTGTGCCT GAAGCGCTGA CGTCCCTGAA AACTGCGCCA 
GCCACCTCCC CTTCAATGCT GCCGCCCCAT GTACCCCTAT TGGTAACAAC ACCTGTGCCA 
ACCATCCATG TGGCCACAGT CCCTTCCCCC ACCGAACATC CGTGTTTGCC AGCAGACGCC 
TTGGCGCAGA CGTCTCACAG TGCATCGCAG ACTTTGACAA CAGAGCTGTC ACAACTCCAA 
ACTCCTGCTG TGGAGAGCAG TCATTCTGAT GTAGCTTCTG GGCTCAGTGA TGGCAATGAG 
GGCGTAACAG GTGGTAGACA TGAGGGTCGT CCTGCCAAAA GACACCAGCG CAGATCGGTG 
CGCAGCCGTT CACGCAATGA AAAGACCTCG AAGGTCAAGC TCAGTGTGCT TAATATCTCC 
AGCATGGGTG ACAGAGTTGC AGAGTGTCAA TTAGAAACAC ACAACAGGAA AATGGTGACT 
TTCAAGTTCG ATCTTGATGG TGATAACCCA GAGGAAATTG CACAGATAAT GGTACAAAGT 
GAATTCATTC TTGAAAGCGA GCGCGAATCG TTCATCGATC GGATCCGAGA AGTGATCGAA 
ACAGCTGATG AGAAGGGGGT GGAGAGAGAG CGCAGCCAGA TGGCCAGTGA TCATGAGCAG 
CTTATGCCCA CAACATCTGC GCCTCTTGAA CACGGTAGAA CTTACAATCT CAGTGTTCCA 
CCTAGTTCAG CTGCACAGGT GGTTCATTCT GCAGGCCGCA GGTTTATCGT GAGTCCAGTG 
CCTGAATCCA GACTGAAGGA TCAGTTCTTT AGCCCATCCC AACCTACAAT CCCACTTCAT 
GAAGAACCTG CCCCTGTGCC AGTTGCATTG CCTGCCTTGG GGCTCTCTGT GTCTGCGTCT 
GCTGTCAGCC TGCAGCAGGC GTTCACTGAG ATGCGTCAAG CCCACTTTGA CCCAGGTCCC 
AGCACTGCAC CCCCTATGCT CCACAACTCC ATGCCCCCTC TGCTTCCTGC CACAACACCC 
TTGCCCTCTA GTGCCACCCC GGTGGTCTCT CCCCCAGTGT CTTCAGAAGT GACCTCTTCG 
CCATCTCTAC CTTCTATAAA CCCTCCAGTG TCATCATCTC CTCCTCCAAC CGTCCCGGTC 
AATTCCGTAA CCTCCCCTCC AGCACAACTC ACCCTGCCTC AAAGTCAAGT TGCGCCTGCC 
GTCATCAGTG TTATTCCCAC CCCTTCCACT CTGCCAATGC CAGCGGTGCC AACTGCCACA 
TCCTTTCCAC AAACTTCAGT CACCGTACCT TCCAGCACAC CAATCGCTCC AATGGGATCA 
GTGTCCGGAG GCAGTGGAGA ACAACCATCC ATCCCTGCTA CTTCTTCGCC CACTAGCGCT 
ACAATCCAAA CGCCACAGCT GGCACCATCC ACCATCCCCA CCACCACGGC TCAGTCACAA 
GTGCCCCCTC TCCAGCCGGT GACCACAACC ATGCCAGTCG TGCAGCCCAC ACCAGTGCAC 
AGCCAACCAC AGACCACCAC CGTGCCCAAT CAGAGCCATG CGCATAGTGT TGAATGCGAC 
AGTGACCAGC AGGGCAAGGT TGATGACATC CAGGCTCTAG ATAAGAAGCT GCGCTCGCTC 
TTTATGGACT TGGGCTCCGG TCCCCCATCT GCCCAGTCTG ATGTCACATC CGACCCGACG 
GCGACTCCAA GCGTTCCTGG CACCTCGTCT CCAACCACAT GTGCCACCCC CAGCGGGACT 
CCACTCCCTC CCTCTAGTTT ACCCCTGAAC TCCTCCACTC AGTCTGCAGG GTCTCCCATG 
ACCTCCATGG GCAATGCCTC CACCCCGGTC GGCTACAGTC AGACCACTCC ATCCAAAGCT 
CCCTTAACAC GATTACCGGT GTTGCCTGTC GGCCCAGATC AGGCTGGCAC GCCGCCCACA 
GAGCACCTTC CACCCTTCCC AGGACCTGGT TTAATTCAGT CCCAGCAGCC CCTGGAGGAT 
CTGGATGCCC AGCTGAGACG GGCACTGAGT CCGGAGACCG TCCCAATCAG CAGTGTCACG 
CACCAGTCAT CTCTAACCGG GATGCCACCT GGAGGACAGC CAGTTCCGTT TTCTCTGGAT 
GATGAGCAAG CAGCCGCACC TGCAGCTGGA GGATACAAAC TGGGGCGATT CCAGGTGTCG 
GTGGCCACTG ATGAGAACAC CACACAGACT CCAACCAGCT CATCCTCTTC CACATCATCT 
TCCTCCACCA CCTCCTCTTC CTCCTCCTCC TGTAGTCTGT CCAGCCCTGA AAACACACTG 
CACAGAACCT CCTCTCCCCT CATAGACACA GGCGCAGATG TGGTCGATGG TCTCCCTGCT 
CTCCCCAACC AGACAACGAC CCAGCCTCAG CCCACCACCA TCGGCCGCTT CCAGGTCACG 
ACCAAAACAG ACACGCAGGT GGGACGCTTC TCTGTTAGCA AAGCACAGGA CGAGGTGACT 
ACATCCACCT TACAACCTCA GCCCACACCT CAAACCCATT CTGCTGTGGG CAACGGTCCA 
GCACAGAGTC CCGGGAGCTT AAACAACTCC ATCAGCTCCT ATTTCAGCAG CGATAATGAC 
TCGGAGTTTG AAGACGAGGA CTTCAAGAGA GAAGTCAGCA GGCTGAGAGA CAAGCACATG 
ATAGAGAGTC AGGCTCTATA TTCTCGTCAG AAAGCAGAGA TTGAGGCTCT GTTTGCCCGT 
TTGGGGAAAG TTCCACCTGC TATGGTTAAC CCACCTGTGG TGAACCCAGC CGGCCGCAGG 
AGACGCCCCA CCAAGAGCAA AAGCAGCAAA TCCAGCCGCG GGAGCTCGCA GGCTAGCAAG 
AGTCCCGTAC AGCCAGCAAC CAGCACTTTA TCAGCACAGA GCGCCCCCTC TGTGTACCCA 
GCCCAGCAGG CTTTCCTGGC CCCCGGGGGG ATGATGGATG GGGGGAGCAG CCCTCTGCTG 
CAGTCTTTCA AACCCTCGCC CTCCAATGAC AACCTGTGCT CCACTTTCAC CAGTGACGCC 
ACGCTCTCTG CGCCCAGTCT CTGTGTAACC TCGCAAGGCT GTGTAAAGTT CAGCTGTGGC 
TCGGAGAGAG TGACCTTCAA ACCAGGTGGC AGGAGGACGC GTTTTCTGAG TACGCCCTGC 
TTGGCTCTTT GGAAGATGGT GAAAAAAGTC TGCCCCTGCA ACCAACTCTG TAGGACGAGC 
AGCACCAACA CGGTGGCAGG ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT 
CAGAGCTGCA AGGGCATGTT CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG 
GACGCCATGA ATCTATCACA GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT 
CAGGGCCACA GCTACGAAAT GGTTCCTCCA GTAAACATGG GCCGTAAGTA CTCAGCTCCG 
GGTCAGCTGT GCCCAAGCAT CGCTTCAACT CTGAGCGGCC ATCCACCAAT GCCCAACACT 
CCCGCTACCT CTCTAGGAGC TCGGAAGGGC TCCTTGTGTC CCACGCCGCA GTACGGCTAC 
CCCTCCGCAC CTTACAGCGC CCAGTGGGCT GGAGCGGCCA CGCACACCCA GGCCGGTCTG 
CTGGCCACCT CACAACCCCT GGGCCAATAT CCCCAGGTGC CCACCCCTTT ACAGACCTTC 
CACATCAGCA CTTTGCAAAA GTCAGTCAGC CACCCGGGTG GACCCAATCT AAAGAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021475204.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330879.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

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CloneID	ODa142110
Clone ID Related Accession (Same CDS sequence)	XM_021475204.1
Accession Version	XM_021475204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GACGAGCAGC ACCAACACGG TGGCAGGACC CGGTCAGAAC 
CAAAGCCAGC CACCCGTCTC TCAGTCTCAG AGCTGCAAGG GCATGTTCAC AGACGAGCTG 
CACAAACTGG TGGACAACTG GGCCCGGGAC GCCATGAACC TATCACAGGG CAAGAGAGGC 
TCCAAACACC AACAGCAAGT GGTTCCTCAG GGCCACAGCT ACGAAATGGT TCCTCCAGTA 
AACATGGGCC GTAAGTACTC AGCTCCGGGT CAGCTGTGCC CAAGCATCGC TTCAACTCTG 
AGCGGCCATC CACCAATGCC CAACACTCCC GCTACCTCTC TAGGAGCTCG GAAGGGCTCC 
TTGTGTCCCA CGCCGCAGTA CGGCTACCCC TCCGCACCTT ACAGCGCCCA GTGGGCTGGA 
GCGGCCACGC ACACCCAGGC CGGTCTGCTG GCCACCTCAC AACCCCTGGG CCAATATCCC 
CAGGTGCCCA CCCCTTTACA GACCTTCCAC ATCAGCACTT TGCAAAAGTC AGTCAGCCAC 
CCGGGCGGAC CCAATCTGAA GAGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330879.1
CDS	1100..8449
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021475204.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475204.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GACGAGCAGC ACCAACACGG TGGCAGGACC CGGTCAGAAC 
CAAAGCCAGC CACCCGTCTC TCAGTCTCAG AGCTGCAAGG GCATGTTCAC AGACGAGCTG 
CACAAACTGG TGGACAACTG GGCCCGGGAC GCCATGAACC TATCACAGGG CAAGAGAGGC 
TCCAAACACC AACAGCAAGT GGTTCCTCAG GGCCACAGCT ACGAAATGGT TCCTCCAGTA 
AACATGGGCC GTAAGTACTC AGCTCCGGGT CAGCTGTGCC CAAGCATCGC TTCAACTCTG 
AGCGGCCATC CACCAATGCC CAACACTCCC GCTACCTCTC TAGGAGCTCG GAAGGGCTCC 
TTGTGTCCCA CGCCGCAGTA CGGCTACCCC TCCGCACCTT ACAGCGCCCA GTGGGCTGGA 
GCGGCCACGC ACACCCAGGC CGGTCTGCTG GCCACCTCAC AACCCCTGGG CCAATATCCC 
CAGGTGCCCA CCCCTTTACA GACCTTCCAC ATCAGCACTT TGCAAAAGTC AGTCAGCCAC 
CCGGGCGGAC CCAATCTGAA GAGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021475206.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330881.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

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CloneID	ODa142111
Clone ID Related Accession (Same CDS sequence)	XM_021475206.1
Accession Version	XM_021475206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GTTGGAGTCA GAGTGATAAA 
AGTGTCACTT TAGCCTCAGA GCGGACGCCC AGGGACGAGC AGCACCAACA CGGTGGCAGG 
ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT CAGAGCTGCA AGGGCATGTT 
CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG GACGCCATGA ACCTATCACA 
GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT CAGGGCCACA GCTACGAAAT 
GGTTCCTCCA GTAAACATGG GCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330881.1
CDS	1101..8207
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRKMVKKVCPCNQLCSWSQSDKSVTLASERTPRDEQHQHGGRTRSEPKPATRLSVSELQGHVHRRAAQTGGQLGPGRHEPITGQERLQTPTASGSSGPQLRNGSSSKHGP

Target ORF information:

RefSeq Version	XM_021475206.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475206.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GTTGGAGTCA GAGTGATAAA 
AGTGTCACTT TAGCCTCAGA GCGGACGCCC AGGGACGAGC AGCACCAACA CGGTGGCAGG 
ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT CAGAGCTGCA AGGGCATGTT 
CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG GACGCCATGA ACCTATCACA 
GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT CAGGGCCACA GCTACGAAAT 
GGTTCCTCCA GTAAACATGG GCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021475207.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330882.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

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CloneID	ODa142112
Clone ID Related Accession (Same CDS sequence)	XM_021475207.1
Accession Version	XM_021475207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330882.1
CDS	1099..7170
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMVQVPIPQSTSGMSLASCSTPAASASQQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021475207.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475207.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471501.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327176.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

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CloneID	ODa144480
Clone ID Related Accession (Same CDS sequence)	XM_021471501.1
Accession Version	XM_021471501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA TCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGTGG ACCCAATCTA 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327176.1
CDS	1098..8489
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471501.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471501.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA TCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGTGG ACCCAATCTA 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471502.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327177.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

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CloneID	ODa144481
Clone ID Related Accession (Same CDS sequence)	XM_021471502.1
Accession Version	XM_021471502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGC GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GCCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACACG GTAGAACTTA CAATCTCAGT 
GTTCCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCGGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCT TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCAGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTTACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTC AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCCTCTGTG 
TACCCAGCCC AGCAGGCTTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAATCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGTGGACC CAATCTAAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327177.1
CDS	1098..8486
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471502.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471502.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGC GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GCCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACACG GTAGAACTTA CAATCTCAGT 
GTTCCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCGGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCT TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCAGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTTACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTC AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCCTCTGTG 
TACCCAGCCC AGCAGGCTTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAATCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGTGGACC CAATCTAAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471503.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327178.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

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CloneID	ODa144482
Clone ID Related Accession (Same CDS sequence)	XM_021471503.1
Accession Version	XM_021471503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTGTTCC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CGGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCTTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCAG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TTACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTCAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCCT CTGTGTACCC AGCCCAGCAG 
GCTTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AATCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGT GGACCCAATC TAAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327178.1
CDS	1098..8471
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471503.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471503.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTGTTCC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CGGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCTTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCAG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TTACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTCAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCCT CTGTGTACCC AGCCCAGCAG 
GCTTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AATCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGT GGACCCAATC TAAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471504.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327179.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

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CloneID	ODa144483
Clone ID Related Accession (Same CDS sequence)	XM_021471504.1
Accession Version	XM_021471504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAT 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGTGGA CCCAATCTAA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327179.1
CDS	1098..8468
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471504.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471504.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAT 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGTGGA CCCAATCTAA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471505.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327180.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

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CloneID	ODa144484
Clone ID Related Accession (Same CDS sequence)	XM_021471505.1
Accession Version	XM_021471505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGTGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGCGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGCC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACACGGTA GAACTTACAA TCTCAGTGTT CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCGGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCTTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC AGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTTACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTCAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC CTCTGTGTAC CCAGCCCAGC AGGCTTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAATCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GTGGACCCAA TCTAAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327180.1
CDS	1098..8453
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471505.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471505.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGTGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGCGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGCC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACACGGTA GAACTTACAA TCTCAGTGTT CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCGGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCTTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC AGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTTACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTCAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC CTCTGTGTAC CCAGCCCAGC AGGCTTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAATCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GTGGACCCAA TCTAAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471506.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327181.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

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CloneID	ODa144485
Clone ID Related Accession (Same CDS sequence)	XM_021471506.1
Accession Version	XM_021471506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GACGAGCAGC ACCAACACGG TGGCAGGACC CGGTCAGAAC 
CAAAGCCAGC CACCCGTCTC TCAGTCTCAG AGCTGCAAGG GCATGTTCAC AGACGAGCTG 
CACAAACTGG TGGACAACTG GGCCCGGGAC GCCATGAATC TATCACAGGG CAAGAGAGGC 
TCCAAACACC AACAGCAAGT GGTTCCTCAG GGCCACAGCT ACGAAATGGT TCCTCCAGTA 
AACATGGGCC GTAAGTACTC AGCTCCGGGT CAGCTGTGCC CAAGCATCGC TTCAACTCTG 
AGCGGCCATC CACCAATGCC CAACACTCCC GCTACCTCTC TAGGAGCTCG GAAGGGCTCC 
TTGTGTCCCA CGCCGCAGTA CGGCTACCCC TCCGCACCTT ACAGCGCCCA GTGGGCTGGA 
GCGGCCACGC ACACCCAGGC CGGTCTGCTG GCCACCTCAC AACCCCTGGG CCAATATCCC 
CAGGTGCCCA CCCCTTTACA GACCTTCCAC ATCAGCACTT TGCAAAAGTC AGTCAGCCAC 
CCGGGTGGAC CCAATCTAAA GAGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327181.1
CDS	1098..8447
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471506.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471506.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GACGAGCAGC ACCAACACGG TGGCAGGACC CGGTCAGAAC 
CAAAGCCAGC CACCCGTCTC TCAGTCTCAG AGCTGCAAGG GCATGTTCAC AGACGAGCTG 
CACAAACTGG TGGACAACTG GGCCCGGGAC GCCATGAATC TATCACAGGG CAAGAGAGGC 
TCCAAACACC AACAGCAAGT GGTTCCTCAG GGCCACAGCT ACGAAATGGT TCCTCCAGTA 
AACATGGGCC GTAAGTACTC AGCTCCGGGT CAGCTGTGCC CAAGCATCGC TTCAACTCTG 
AGCGGCCATC CACCAATGCC CAACACTCCC GCTACCTCTC TAGGAGCTCG GAAGGGCTCC 
TTGTGTCCCA CGCCGCAGTA CGGCTACCCC TCCGCACCTT ACAGCGCCCA GTGGGCTGGA 
GCGGCCACGC ACACCCAGGC CGGTCTGCTG GCCACCTCAC AACCCCTGGG CCAATATCCC 
CAGGTGCCCA CCCCTTTACA GACCTTCCAC ATCAGCACTT TGCAAAAGTC AGTCAGCCAC 
CCGGGTGGAC CCAATCTAAA GAGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471507.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327182.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

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CloneID	ODa144486
Clone ID Related Accession (Same CDS sequence)	XM_021471507.1
Accession Version	XM_021471507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAATCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGTGGACC CAATCTAAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327182.1
CDS	1098..8426
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471507.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471507.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAATCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGTGGACC CAATCTAAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471508.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327183.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

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CloneID	ODa144487
Clone ID Related Accession (Same CDS sequence)	XM_021471508.1
Accession Version	XM_021471508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTTA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TCAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCCTCTG TGTACCCAGC CCAGCAGGCT 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAT 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGTGGA CCCAATCTAA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327183.1
CDS	1098..8408
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471508.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471508.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTTA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TCAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCCTCTG TGTACCCAGC CCAGCAGGCT 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAT 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGTGGA CCCAATCTAA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471509.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327184.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X17, mRNA.

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CloneID	ODa144488
Clone ID Related Accession (Same CDS sequence)	XM_021471509.1
Accession Version	XM_021471509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAA GATGGTGAAA 
AAAGTCTGCC CCTGCAACCA ACTCTGTAGG ACGAGCAGCA CCAACACGGT GGCAGGACCC 
GGTCAGAACC AAAGCCAGCC ACCCGTCTCT CAGTCTCAGA GCTGCAAGGG CATGTTCACA 
GACGAGCTGC ACAAACTGGT GGACAACTGG GCCCGGGACG CCATGAATCT ATCACAGGGC 
AAGAGAGGCT CCAAACACCA ACAGCAAGTG GTTCCTCAGG GCCACAGCTA CGAAATGGTT 
CCTCCAGTAA ACATGGGCCG TAAGTACTCA GCTCCGGGTC AGCTGTGCCC AAGCATCGCT 
TCAACTCTGA GCGGCCATCC ACCAATGCCC AACACTCCCG CTACCTCTCT AGGAGCTCGG 
AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC GGCTACCCCT CCGCACCTTA CAGCGCCCAG 
TGGGCTGGAG CGGCCACGCA CACCCAGGCC GGTCTGCTGG CCACCTCACA ACCCCTGGGC 
CAATATCCCC AGGTGCCCAC CCCTTTACAG ACCTTCCACA TCAGCACTTT GCAAAAGTCA 
GTCAGCCACC CGGGTGGACC CAATCTAAAG AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327184.1
CDS	1098..8396
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471509.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471509.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAA GATGGTGAAA 
AAAGTCTGCC CCTGCAACCA ACTCTGTAGG ACGAGCAGCA CCAACACGGT GGCAGGACCC 
GGTCAGAACC AAAGCCAGCC ACCCGTCTCT CAGTCTCAGA GCTGCAAGGG CATGTTCACA 
GACGAGCTGC ACAAACTGGT GGACAACTGG GCCCGGGACG CCATGAATCT ATCACAGGGC 
AAGAGAGGCT CCAAACACCA ACAGCAAGTG GTTCCTCAGG GCCACAGCTA CGAAATGGTT 
CCTCCAGTAA ACATGGGCCG TAAGTACTCA GCTCCGGGTC AGCTGTGCCC AAGCATCGCT 
TCAACTCTGA GCGGCCATCC ACCAATGCCC AACACTCCCG CTACCTCTCT AGGAGCTCGG 
AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC GGCTACCCCT CCGCACCTTA CAGCGCCCAG 
TGGGCTGGAG CGGCCACGCA CACCCAGGCC GGTCTGCTGG CCACCTCACA ACCCCTGGGC 
CAATATCCCC AGGTGCCCAC CCCTTTACAG ACCTTCCACA TCAGCACTTT GCAAAAGTCA 
GTCAGCCACC CGGGTGGACC CAATCTAAAG AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471510.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327185.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

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CloneID	ODa144489
Clone ID Related Accession (Same CDS sequence)	XM_021471510.1
Accession Version	XM_021471510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAATCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGTGGACCCA ATCTAAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327185.1
CDS	1098..8354
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471510.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471510.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAATCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGTGGACCCA ATCTAAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471511.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327186.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

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CloneID	ODa144490
Clone ID Related Accession (Same CDS sequence)	XM_021471511.1
Accession Version	XM_021471511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGTGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGCGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGC 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACACGGT 
AGAACTTACA ATCTCAGTGT TCCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCGGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCTTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CAGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTTACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTCAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CCTCTGTGTA CCCAGCCCAG CAGGCTTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAATCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGTGGACCCA ATCTAAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327186.1
CDS	1098..8264
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471511.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471511.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGTGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGCGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGC 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACACGGT 
AGAACTTACA ATCTCAGTGT TCCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCGGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCTTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CAGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTTACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTCAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CCTCTGTGTA CCCAGCCCAG CAGGCTTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAATCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGTGGACCCA ATCTAAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471512.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327187.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

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CloneID	ODa144491
Clone ID Related Accession (Same CDS sequence)	XM_021471512.1
Accession Version	XM_021471512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGTGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGCGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGCCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACACGGTAGA 
ACTTACAATC TCAGTGTTCC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CGGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCTTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCAG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TTACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTCAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCCT CTGTGTACCC AGCCCAGCAG GCTTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AATCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGT 
GGACCCAATC TAAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327187.1
CDS	1098..8261
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471512.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471512.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGTGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGCGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGCCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACACGGTAGA 
ACTTACAATC TCAGTGTTCC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CGGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCTTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCAG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TTACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTCAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCCT CTGTGTACCC AGCCCAGCAG GCTTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AATCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGT 
GGACCCAATC TAAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_017355253.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210742.2 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

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CloneID	ODa144492
Clone ID Related Accession (Same CDS sequence)	XM_017355253.2
Accession Version	XM_017355253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AATCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210742.2
CDS	1099..8226
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCSWSQSDKSVTLASERTPRDEQHQHGGRTRSEPKPATRLSVSELQGHVHRRAAQTGGQLGPGRHESITGQERLQTPTASGSSGPQLRNGSSSKHGP

Target ORF information:

RefSeq Version	XM_017355253.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355253.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AATCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471513.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327188.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

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CloneID	ODa144493
Clone ID Related Accession (Same CDS sequence)	XM_021471513.1
Accession Version	XM_021471513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GTTGGAGTCA GAGTGATAAA 
AGTGTCACTT TAGCCTCAGA GCGGACGCCC AGGGACGAGC AGCACCAACA CGGTGGCAGG 
ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT CAGAGCTGCA AGGGCATGTT 
CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG GACGCCATGA ATCTATCACA 
GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT CAGGGCCACA GCTACGAAAT 
GGTTCCTCCA GTAAACATGG GCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327188.1
CDS	1099..8205
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRKMVKKVCPCNQLCSWSQSDKSVTLASERTPRDEQHQHGGRTRSEPKPATRLSVSELQGHVHRRAAQTGGQLGPGRHESITGQERLQTPTASGSSGPQLRNGSSSKHGP

Target ORF information:

RefSeq Version	XM_021471513.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471513.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACTGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT GCCTCTTCCT CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCCGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCACACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GTTGGAGTCA GAGTGATAAA 
AGTGTCACTT TAGCCTCAGA GCGGACGCCC AGGGACGAGC AGCACCAACA CGGTGGCAGG 
ACCCGGTCAG AACCAAAGCC AGCCACCCGT CTCTCAGTCT CAGAGCTGCA AGGGCATGTT 
CACAGACGAG CTGCACAAAC TGGTGGACAA CTGGGCCCGG GACGCCATGA ATCTATCACA 
GGGCAAGAGA GGCTCCAAAC ACCAACAGCA AGTGGTTCCT CAGGGCCACA GCTACGAAAT 
GGTTCCTCCA GTAAACATGG GCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471514.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327189.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.

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CloneID	ODa144494
Clone ID Related Accession (Same CDS sequence)	XM_021471514.1
Accession Version	XM_021471514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGTGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGCG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG CCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACACGG TAGAACTTAC AATCTCAGTG TTCCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCGGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCTT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCAGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTTACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTCA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCCTCTGTGT ACCCAGCCCA GCAGGCTTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAATCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGTGGACCC AATCTAAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327189.1
CDS	1097..7204
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471514.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471514.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGTGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGCG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG CCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACACGG TAGAACTTAC AATCTCAGTG TTCCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCGGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCTT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCAGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTTACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTCA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCCTCTGTGT ACCCAGCCCA GCAGGCTTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAATCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGTGGACCC AATCTAAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021471515.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021327190.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

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CloneID	ODa144495
Clone ID Related Accession (Same CDS sequence)	XM_021471515.1
Accession Version	XM_021471515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394549.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA TCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGTGG ACCCAATCTA 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021327190.1
CDS	1097..7168
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMVQVPIPQSTSGMSLASCSTPAASASQQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSFPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTLQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_021471515.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471515.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGCG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGACGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCTCT GATCTCCCGC 
AAACGAGAAC AACGACAACA AGTGCGAGCC GAGCAGGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCTCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGT GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGCGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGCCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ACGGTAGAAC TTACAATCTC 
AGTGTTCCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCG GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCTTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCAGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTT ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTCAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCCTCT 
GTGTACCCAG CCCAGCAGGC TTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA TCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGTGG ACCCAATCTA 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_017355253.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210742.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

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CloneID	ODa58533
Clone ID Related Accession (Same CDS sequence)	XM_017355253.1 , XM_021475205.1
Accession Version	XM_017355253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210742.1
CDS	1164..8291
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCSWSQSDKSVTLASERTPRDEQHQHGGRTRSEPKPATRLSVSELQGHVHRRAAQTGGQLGPGRHEPITGQERLQTPTASGSSGPQLRNGSSSKHGP

Target ORF information:

RefSeq Version	XM_017355253.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355253.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_021475205.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330880.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

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CloneID	ODa58533
Clone ID Related Accession (Same CDS sequence)	XM_017355253.1 , XM_021475205.1
Accession Version	XM_021475205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330880.1
CDS	1101..8228
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCSWSQSDKSVTLASERTPRDEQHQHGGRTRSEPKPATRLSVSELQGHVHRRAAQTGGQLGPGRHEPITGQERLQTPTASGSSGPQLRNGSSSKHGP

Target ORF information:

RefSeq Version	XM_021475205.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475205.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGTTGGAGTC AGAGTGATAA AAGTGTCACT TTAGCCTCAG AGCGGACGCC CAGGGACGAG 
CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC 
TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG 
GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC 
TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_017355254.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210743.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

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CloneID	ODa58534
Clone ID Related Accession (Same CDS sequence)	XM_017355254.1
Accession Version	XM_017355254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTA TGGTGAAAAA AGTCTGCCCC TGCAACCAAC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210743.1
CDS	1164..8000
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALYGEKSLPLQPTL

Target ORF information:

RefSeq Version	XM_017355254.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355254.1

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTA TGGTGAAAAA AGTCTGCCCC TGCAACCAAC TCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_005164548.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164605.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

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CloneID	ODa09175
Clone ID Related Accession (Same CDS sequence)	XM_005164548.3 , XM_005164548.4
Accession Version	XM_005164548.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164548.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTGTACC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CAGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCCTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCGG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TCACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTTAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCTT CTGTGTACCC AGCCCAGCAG 
GCCTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGC GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164605.1
CDS	1163..8536
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_005164548.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164548.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTGTACC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CAGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCCTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCGG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TCACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTTAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCTT CTGTGTACCC AGCCCAGCAG 
GCCTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGC GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_005164548.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164605.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

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CloneID	ODa09175
Clone ID Related Accession (Same CDS sequence)	XM_005164548.3 , XM_005164548.4
Accession Version	XM_005164548.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164548.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTGTACC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CAGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCCTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCGG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TCACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTTAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCTT CTGTGTACCC AGCCCAGCAG 
GCCTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGC GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164605.1
CDS	1100..8473
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_005164548.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164548.4

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTGTACC ACCTAGTTCA 
GCTGCACAGG TGGTTCATTC TGCAGGCCGC AGGTTTATCG TGAGTCCAGT GCCTGAATCC 
AGACTGAAGG ATCAGTTCTT TAGCCCATCC CAACCTACAA TCCCACTTCA TGAAGAACCT 
GCCCCTGTGC CAGTTGCATT GCCTGCCTTG GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC 
CTGCAGCAGG CGTTCACTGA GATGCGTCAA GCCCACTTTG ACCCAGGTCC CAGCACTGCA 
CCCCCTATGC TCCACAACTC CATGCCCCCT CTGCTTCCTG CCACAACACC CTTGCCCTCT 
AGTGCCACCC CAGTGGTCTC TCCCCCAGTG TCTTCAGAAG TGACCTCTTC GCCATCTCTA 
CCTTCTATAA ACCCTCCAGT GTCATCATCT CCTCCTCCAA CCGTCCCGGT CAATTCCGTA 
ACCTCCCCTC CAGCACAACT CACCCTGCCT CAAAGTCAAG TTGCGCCTGC CGTCATCAGT 
GTTATTCCCA CCCCTTCCAC TCTGCCAATG CCAGCGGTGC CAACTGCCAC ATCCCTTCCA 
CAAACTTCAG TCACCGTACC TTCCAGCACA CCAATCGCTC CAATGGGATC AGTGTCCGGA 
GGCAGTGGAG AACAACCATC CATCCCTGCT ACTTCTTCGC CCACTAGCGC TACAATCCAA 
ACGCCACAGC TGGCACCATC CACCATCCCC ACCACCACGG CTCAGTCACA AGTGCCCCCT 
CTCCAGCCGG TGACCACAAC CATGCCAGTC GTGCAGCCCA CACCAGTGCA CAGCCAACCA 
CAGACCACCA CCGTGCCCAA TCAGAGCCAT GCGCATAGTG TTGAATGCGA CAGTGACCAG 
CAGGGCAAGG TTGATGACAT CCAGGCTCTA GATAAGAAGC TGCGCTCGCT CTTTATGGAC 
TTGGGCTCCG GTCCCCCATC TGCCCAGTCT GATGTCACAT CCGACCCGAC GGCGACTCCA 
AGCGTTCCTG GCACCTCGTC TCCAACCACA TGTGCCACCC CCAGCGGGAC TCCACTCCCT 
CCCTCTAGTT TACCCCTGAA CTCCTCCACT CAGTCTGCGG GGTCTCCCAT GACCTCCATG 
GGCAATGCCT CCACCCCGGT CGGCTACAGT CAGACCACTC CATCCAAAGC TCCCTTAACA 
CGATTACCGG TGTTGCCTGT CGGCCCAGAT CAGGCTGGCA CGCCGCCCAC AGAGCACCTT 
CCACCCTTCC CAGGACCTGG TTTAATTCAG TCCCAGCAGC CCCTGGAGGA TCTGGATGCC 
CAGCTGAGAC GGGCACTGAG TCCGGAGACC GTCCCAATCA GCAGTGTCAC GCACCAGTCA 
TCTCTAACCG GGATGCCACC TGGAGGACAG CCAGTTCCGT TTTCTCTGGA TGATGAGCAA 
GCAGCCGCAC CTGCAGCTGG AGGATACAAA CTGGGGCGAT TCCAGGTGTC GGTGGCCACT 
GATGAGAACA CCACACAGAC TCCAACCAGC TCATCCTCTT CCACATCATC TTCCTCCACC 
ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG TCCAGCCCTG AAAACACACT GCACAGAACC 
TCCTCTCCCC TCATAGACAC AGGCGCAGAT GTGGTCGATG GTCTCCCTGC TCTCCCCAAC 
CAGACAACGA CCCAGCCTCA GCCCACCACC ATCGGCCGCT TCCAGGTCAC GACCAAAACA 
GACACGCAGG TGGGACGCTT CTCTGTTAGC AAAGCACAGG ACGAGGTGAC TACATCCACC 
TCACAACCTC AGCCCACACC TCAAACCCAT TCTGCTGTGG GCAACGGTCC AGCACAGAGT 
CCCGGGAGCT TAAACAACTC CATCAGCTCC TATTTCAGCA GCGATAATGA CTCGGAGTTT 
GAAGACGAGG ACTTTAAGAG AGAAGTCAGC AGGCTGAGAG ACAAGCACAT GATAGAGAGT 
CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA 
GTTCCACCTG CTATGGTTAA CCCACCTGTG GTGAACCCAG CCGGCCGCAG GAGACGCCCC 
ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA 
CAGCCAGCAA CCAGCACTTT ATCAGCACAG AGCGCCCCTT CTGTGTACCC AGCCCAGCAG 
GCCTTCCTGG CCCCCGGGGG GATGATGGAT GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC 
AAACCCTCGC CCTCCAATGA CAACCTGTGC TCCACTTTCA CCAGTGACGC CACGCTCTCT 
GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA 
GTGACCTTCA AACCAGGTGG CAGGAGGACG CGTTTTCTGA GTACGCCCTG CTTGGCTCTT 
TGGAAGATGG TGAAAAAAGT CTGCCCCTGC AACCAACTCT GTAGGACGAG CAGCACCAAC 
ACGGTGGCAG GACCCGGTCA GAACCAAAGC CAGCCACCCG TCTCTCAGTC TCAGAGCTGC 
AAGGGCATGT TCACAGACGA GCTGCACAAA CTGGTGGACA ACTGGGCCCG GGACGCCATG 
AACCTATCAC AGGGCAAGAG AGGCTCCAAA CACCAACAGC AAGTGGTTCC TCAGGGCCAC 
AGCTACGAAA TGGTTCCTCC AGTAAACATG GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG 
TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC CATCCACCAA TGCCCAACAC TCCCGCTACC 
TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT CCCACGCCGC AGTACGGCTA CCCCTCCGCA 
CCTTACAGCG CCCAGTGGGC TGGAGCGGCC ACGCACACCC AGGCCGGTCT GCTGGCCACC 
TCACAACCCC TGGGCCAATA TCCCCAGGTG CCCACCCCTT TACAGACCTT CCACATCAGC 
ACTTTGCAAA AGTCAGTCAG CCACCCGGGC GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300083.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298358.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

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CloneID	ODa43442
Clone ID Related Accession (Same CDS sequence)	XM_009300083.3 , XM_009300084.2 , XM_009300083.2
Accession Version	XM_009300083.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298358.1
CDS	1100..8491
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300083.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300083.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300084.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298359.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

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CloneID	ODa43442
Clone ID Related Accession (Same CDS sequence)	XM_009300083.3 , XM_009300084.2 , XM_009300083.2
Accession Version	XM_009300084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298359.1
CDS	545..7936
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300084.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300084.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300083.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298358.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

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CloneID	ODa43442
Clone ID Related Accession (Same CDS sequence)	XM_009300083.3 , XM_009300084.2 , XM_009300083.2
Accession Version	XM_009300083.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298358.1
CDS	1164..8555
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300083.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300083.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGT 
ACGCCCTGCT TGGCTCTTTG GAAGATGGTG AAAAAAGTCT GCCCCTGCAA CCAACTCTGT 
AGGACGAGCA GCACCAACAC GGTGGCAGGA CCCGGTCAGA ACCAAAGCCA GCCACCCGTC 
TCTCAGTCTC AGAGCTGCAA GGGCATGTTC ACAGACGAGC TGCACAAACT GGTGGACAAC 
TGGGCCCGGG ACGCCATGAA CCTATCACAG GGCAAGAGAG GCTCCAAACA CCAACAGCAA 
GTGGTTCCTC AGGGCCACAG CTACGAAATG GTTCCTCCAG TAAACATGGG CCGTAAGTAC 
TCAGCTCCGG GTCAGCTGTG CCCAAGCATC GCTTCAACTC TGAGCGGCCA TCCACCAATG 
CCCAACACTC CCGCTACCTC TCTAGGAGCT CGGAAGGGCT CCTTGTGTCC CACGCCGCAG 
TACGGCTACC CCTCCGCACC TTACAGCGCC CAGTGGGCTG GAGCGGCCAC GCACACCCAG 
GCCGGTCTGC TGGCCACCTC ACAACCCCTG GGCCAATATC CCCAGGTGCC CACCCCTTTA 
CAGACCTTCC ACATCAGCAC TTTGCAAAAG TCAGTCAGCC ACCCGGGCGG ACCCAATCTG 
AAGAGCACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300085.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298360.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

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CloneID	ODa43443
Clone ID Related Accession (Same CDS sequence)	XM_009300085.3 , XM_009300085.2
Accession Version	XM_009300085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGT GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GTCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACATG GTAGAACTTA CAATCTCAGT 
GTACCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCAGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCC TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCGGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTCACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTT AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCTTCTGTG 
TACCCAGCCC AGCAGGCCTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298360.1
CDS	1100..8488
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300085.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300085.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGT GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GTCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACATG GTAGAACTTA CAATCTCAGT 
GTACCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCAGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCC TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCGGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTCACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTT AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCTTCTGTG 
TACCCAGCCC AGCAGGCCTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300085.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298360.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

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CloneID	ODa43443
Clone ID Related Accession (Same CDS sequence)	XM_009300085.3 , XM_009300085.2
Accession Version	XM_009300085.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGT GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GTCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACATG GTAGAACTTA CAATCTCAGT 
GTACCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCAGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCC TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCGGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTCACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTT AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCTTCTGTG 
TACCCAGCCC AGCAGGCCTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298360.1
CDS	1164..8552
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300085.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300085.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGTCAT TCTGATGTAG CTTCTGGGCT CAGTGATGGC 
AATGAGGGCG TAACAGGTGG TAGACATGAG GGTCGTCCTG CCAAAAGACA CCAGCGCAGA 
TCGGTGCGCA GCCGTTCACG CAATGAAAAG ACCTCGAAGG TCAAGCTCAG TGTGCTTAAT 
ATCTCCAGCA TGGGTGACAG AGTTGCAGAG TGTCAATTAG AAACACACAA CAGGAAAATG 
GTGACTTTCA AGTTCGATCT TGATGGTGAT AACCCAGAGG AAATTGCACA GATAATGGTA 
CAAAGTGAAT TCATTCTTGA AAGCGAGCGT GAATCGTTCA TCGATCGGAT CCGAGAAGTG 
ATCGAAACAG CTGATGAGAA GGGGGTGGAG AGAGAGCGCA GTCAGATGGC CAGTGATCAT 
GAGCAGCTTA TGCCCACAAC ATCTGCGCCT CTTGAACATG GTAGAACTTA CAATCTCAGT 
GTACCACCTA GTTCAGCTGC ACAGGTGGTT CATTCTGCAG GCCGCAGGTT TATCGTGAGT 
CCAGTGCCTG AATCCAGACT GAAGGATCAG TTCTTTAGCC CATCCCAACC TACAATCCCA 
CTTCATGAAG AACCTGCCCC TGTGCCAGTT GCATTGCCTG CCTTGGGGCT CTCTGTGTCT 
GCGTCTGCTG TCAGCCTGCA GCAGGCGTTC ACTGAGATGC GTCAAGCCCA CTTTGACCCA 
GGTCCCAGCA CTGCACCCCC TATGCTCCAC AACTCCATGC CCCCTCTGCT TCCTGCCACA 
ACACCCTTGC CCTCTAGTGC CACCCCAGTG GTCTCTCCCC CAGTGTCTTC AGAAGTGACC 
TCTTCGCCAT CTCTACCTTC TATAAACCCT CCAGTGTCAT CATCTCCTCC TCCAACCGTC 
CCGGTCAATT CCGTAACCTC CCCTCCAGCA CAACTCACCC TGCCTCAAAG TCAAGTTGCG 
CCTGCCGTCA TCAGTGTTAT TCCCACCCCT TCCACTCTGC CAATGCCAGC GGTGCCAACT 
GCCACATCCC TTCCACAAAC TTCAGTCACC GTACCTTCCA GCACACCAAT CGCTCCAATG 
GGATCAGTGT CCGGAGGCAG TGGAGAACAA CCATCCATCC CTGCTACTTC TTCGCCCACT 
AGCGCTACAA TCCAAACGCC ACAGCTGGCA CCATCCACCA TCCCCACCAC CACGGCTCAG 
TCACAAGTGC CCCCTCTCCA GCCGGTGACC ACAACCATGC CAGTCGTGCA GCCCACACCA 
GTGCACAGCC AACCACAGAC CACCACCGTG CCCAATCAGA GCCATGCGCA TAGTGTTGAA 
TGCGACAGTG ACCAGCAGGG CAAGGTTGAT GACATCCAGG CTCTAGATAA GAAGCTGCGC 
TCGCTCTTTA TGGACTTGGG CTCCGGTCCC CCATCTGCCC AGTCTGATGT CACATCCGAC 
CCGACGGCGA CTCCAAGCGT TCCTGGCACC TCGTCTCCAA CCACATGTGC CACCCCCAGC 
GGGACTCCAC TCCCTCCCTC TAGTTTACCC CTGAACTCCT CCACTCAGTC TGCGGGGTCT 
CCCATGACCT CCATGGGCAA TGCCTCCACC CCGGTCGGCT ACAGTCAGAC CACTCCATCC 
AAAGCTCCCT TAACACGATT ACCGGTGTTG CCTGTCGGCC CAGATCAGGC TGGCACGCCG 
CCCACAGAGC ACCTTCCACC CTTCCCAGGA CCTGGTTTAA TTCAGTCCCA GCAGCCCCTG 
GAGGATCTGG ATGCCCAGCT GAGACGGGCA CTGAGTCCGG AGACCGTCCC AATCAGCAGT 
GTCACGCACC AGTCATCTCT AACCGGGATG CCACCTGGAG GACAGCCAGT TCCGTTTTCT 
CTGGATGATG AGCAAGCAGC CGCACCTGCA GCTGGAGGAT ACAAACTGGG GCGATTCCAG 
GTGTCGGTGG CCACTGATGA GAACACCACA CAGACTCCAA CCAGCTCATC CTCTTCCACA 
TCATCTTCCT CCACCACCTC CTCTTCCTCC TCCTCCTGTA GTCTGTCCAG CCCTGAAAAC 
ACACTGCACA GAACCTCCTC TCCCCTCATA GACACAGGCG CAGATGTGGT CGATGGTCTC 
CCTGCTCTCC CCAACCAGAC AACGACCCAG CCTCAGCCCA CCACCATCGG CCGCTTCCAG 
GTCACGACCA AAACAGACAC GCAGGTGGGA CGCTTCTCTG TTAGCAAAGC ACAGGACGAG 
GTGACTACAT CCACCTCACA ACCTCAGCCC ACACCTCAAA CCCATTCTGC TGTGGGCAAC 
GGTCCAGCAC AGAGTCCCGG GAGCTTAAAC AACTCCATCA GCTCCTATTT CAGCAGCGAT 
AATGACTCGG AGTTTGAAGA CGAGGACTTT AAGAGAGAAG TCAGCAGGCT GAGAGACAAG 
CACATGATAG AGAGTCAGGC TCTATATTCT CGTCAGAAAG CAGAGATTGA GGCTCTGTTT 
GCCCGTTTGG GGAAAGTTCC ACCTGCTATG GTTAACCCAC CTGTGGTGAA CCCAGCCGGC 
CGCAGGAGAC GCCCCACCAA GAGCAAAAGC AGCAAATCCA GCCGCGGGAG CTCGCAGGCT 
AGCAAGAGTC CCGTACAGCC AGCAACCAGC ACTTTATCAG CACAGAGCGC CCCTTCTGTG 
TACCCAGCCC AGCAGGCCTT CCTGGCCCCC GGGGGGATGA TGGATGGGGG GAGCAGCCCT 
CTGCTGCAGT CTTTCAAACC CTCGCCCTCC AATGACAACC TGTGCTCCAC TTTCACCAGT 
GACGCCACGC TCTCTGCGCC CAGTCTCTGT GTAACCTCGC AAGGCTGTGT AAAGTTCAGC 
TGTGGCTCGG AGAGAGTGAC CTTCAAACCA GGTGGCAGGA GGACGCGTTT TCTGAGTACG 
CCCTGCTTGG CTCTTTGGAA GATGGTGAAA AAAGTCTGCC CCTGCAACCA ACTCTGTAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300086.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298361.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

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CloneID	ODa43444
Clone ID Related Accession (Same CDS sequence)	XM_009300086.2 , XM_009300086.3
Accession Version	XM_009300086.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298361.1
CDS	1163..8533
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300086.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300086.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300086.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298361.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

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CloneID	ODa43444
Clone ID Related Accession (Same CDS sequence)	XM_009300086.2 , XM_009300086.3
Accession Version	XM_009300086.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298361.1
CDS	1100..8470
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300086.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300086.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300087.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298362.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

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CloneID	ODa43445
Clone ID Related Accession (Same CDS sequence)	XM_009300087.3 , XM_009300087.2
Accession Version	XM_009300087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGCGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGTGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGTC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACATGGTA GAACTTACAA TCTCAGTGTA CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCAGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCCTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC GGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTCACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTTAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC TTCTGTGTAC CCAGCCCAGC AGGCCTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAACCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GCGGACCCAA TCTGAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298362.1
CDS	1100..8455
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300087.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300087.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGCGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGTGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGTC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACATGGTA GAACTTACAA TCTCAGTGTA CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCAGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCCTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC GGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTCACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTTAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC TTCTGTGTAC CCAGCCCAGC AGGCCTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAACCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GCGGACCCAA TCTGAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300087.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298362.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

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CloneID	ODa43445
Clone ID Related Accession (Same CDS sequence)	XM_009300087.3 , XM_009300087.2
Accession Version	XM_009300087.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGCGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGTGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGTC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACATGGTA GAACTTACAA TCTCAGTGTA CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCAGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCCTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC GGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTCACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTTAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC TTCTGTGTAC CCAGCCCAGC AGGCCTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAACCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GCGGACCCAA TCTGAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298362.1
CDS	1164..8519
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300087.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300087.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGGCTCCTGT TCAGCAGCCG 
GCCTCTCTAG GTCCCACTTC CCAACCCTGT GCCTCGCTAC CCCAACAACA AACACCCCAA 
CAGCCTTCCA GCACTGTAGC TCAGAGCATC TCCCAAACGC AAATCACGCC AACACAGCTT 
TGCAGTCCGG CTCTCTGCGT TCCAACTATA GCAGTCACCC CGTTTCAGCA GCAACCTGTG 
CCTGAAGCGC TGACGTCCCT GAAAACTGCG CCAGCCACCT CCCCTTCAAT GCTGCCGCCC 
CATGTACCCC TATTGGTAAC AACACCTGTG CCAACCATCC ATGTGGCCAC AGTCCCTTCC 
CCCACCGAAC ATCCGTGTTT GCCAGCAGAC GCCTTGGCGC AGACGTCTCA CAGCGCATCG 
CAGACTTTGA CAACAGAGCT GTCACAACTC CAAACTCCTG CTGTGGAGAG CAGTCATTCT 
GATGTAGCTT CTGGGCTCAG TGATGGCAAT GAGGGCGTAA CAGGTGGTAG ACATGAGGGT 
CGTCCTGCCA AAAGACACCA GCGCAGATCG GTGCGCAGCC GTTCACGCAA TGAAAAGACC 
TCGAAGGTCA AGCTCAGTGT GCTTAATATC TCCAGCATGG GTGACAGAGT TGCAGAGTGT 
CAATTAGAAA CACACAACAG GAAAATGGTG ACTTTCAAGT TCGATCTTGA TGGTGATAAC 
CCAGAGGAAA TTGCACAGAT AATGGTACAA AGTGAATTCA TTCTTGAAAG CGAGCGTGAA 
TCGTTCATCG ATCGGATCCG AGAAGTGATC GAAACAGCTG ATGAGAAGGG GGTGGAGAGA 
GAGCGCAGTC AGATGGCCAG TGATCATGAG CAGCTTATGC CCACAACATC TGCGCCTCTT 
GAACATGGTA GAACTTACAA TCTCAGTGTA CCACCTAGTT CAGCTGCACA GGTGGTTCAT 
TCTGCAGGCC GCAGGTTTAT CGTGAGTCCA GTGCCTGAAT CCAGACTGAA GGATCAGTTC 
TTTAGCCCAT CCCAACCTAC AATCCCACTT CATGAAGAAC CTGCCCCTGT GCCAGTTGCA 
TTGCCTGCCT TGGGGCTCTC TGTGTCTGCG TCTGCTGTCA GCCTGCAGCA GGCGTTCACT 
GAGATGCGTC AAGCCCACTT TGACCCAGGT CCCAGCACTG CACCCCCTAT GCTCCACAAC 
TCCATGCCCC CTCTGCTTCC TGCCACAACA CCCTTGCCCT CTAGTGCCAC CCCAGTGGTC 
TCTCCCCCAG TGTCTTCAGA AGTGACCTCT TCGCCATCTC TACCTTCTAT AAACCCTCCA 
GTGTCATCAT CTCCTCCTCC AACCGTCCCG GTCAATTCCG TAACCTCCCC TCCAGCACAA 
CTCACCCTGC CTCAAAGTCA AGTTGCGCCT GCCGTCATCA GTGTTATTCC CACCCCTTCC 
ACTCTGCCAA TGCCAGCGGT GCCAACTGCC ACATCCCTTC CACAAACTTC AGTCACCGTA 
CCTTCCAGCA CACCAATCGC TCCAATGGGA TCAGTGTCCG GAGGCAGTGG AGAACAACCA 
TCCATCCCTG CTACTTCTTC GCCCACTAGC GCTACAATCC AAACGCCACA GCTGGCACCA 
TCCACCATCC CCACCACCAC GGCTCAGTCA CAAGTGCCCC CTCTCCAGCC GGTGACCACA 
ACCATGCCAG TCGTGCAGCC CACACCAGTG CACAGCCAAC CACAGACCAC CACCGTGCCC 
AATCAGAGCC ATGCGCATAG TGTTGAATGC GACAGTGACC AGCAGGGCAA GGTTGATGAC 
ATCCAGGCTC TAGATAAGAA GCTGCGCTCG CTCTTTATGG ACTTGGGCTC CGGTCCCCCA 
TCTGCCCAGT CTGATGTCAC ATCCGACCCG ACGGCGACTC CAAGCGTTCC TGGCACCTCG 
TCTCCAACCA CATGTGCCAC CCCCAGCGGG ACTCCACTCC CTCCCTCTAG TTTACCCCTG 
AACTCCTCCA CTCAGTCTGC GGGGTCTCCC ATGACCTCCA TGGGCAATGC CTCCACCCCG 
GTCGGCTACA GTCAGACCAC TCCATCCAAA GCTCCCTTAA CACGATTACC GGTGTTGCCT 
GTCGGCCCAG ATCAGGCTGG CACGCCGCCC ACAGAGCACC TTCCACCCTT CCCAGGACCT 
GGTTTAATTC AGTCCCAGCA GCCCCTGGAG GATCTGGATG CCCAGCTGAG ACGGGCACTG 
AGTCCGGAGA CCGTCCCAAT CAGCAGTGTC ACGCACCAGT CATCTCTAAC CGGGATGCCA 
CCTGGAGGAC AGCCAGTTCC GTTTTCTCTG GATGATGAGC AAGCAGCCGC ACCTGCAGCT 
GGAGGATACA AACTGGGGCG ATTCCAGGTG TCGGTGGCCA CTGATGAGAA CACCACACAG 
ACTCCAACCA GCTCATCCTC TTCCACATCA TCTTCCTCCA CCACCTCCTC TTCCTCCTCC 
TCCTGTAGTC TGTCCAGCCC TGAAAACACA CTGCACAGAA CCTCCTCTCC CCTCATAGAC 
ACAGGCGCAG ATGTGGTCGA TGGTCTCCCT GCTCTCCCCA ACCAGACAAC GACCCAGCCT 
CAGCCCACCA CCATCGGCCG CTTCCAGGTC ACGACCAAAA CAGACACGCA GGTGGGACGC 
TTCTCTGTTA GCAAAGCACA GGACGAGGTG ACTACATCCA CCTCACAACC TCAGCCCACA 
CCTCAAACCC ATTCTGCTGT GGGCAACGGT CCAGCACAGA GTCCCGGGAG CTTAAACAAC 
TCCATCAGCT CCTATTTCAG CAGCGATAAT GACTCGGAGT TTGAAGACGA GGACTTTAAG 
AGAGAAGTCA GCAGGCTGAG AGACAAGCAC ATGATAGAGA GTCAGGCTCT ATATTCTCGT 
CAGAAAGCAG AGATTGAGGC TCTGTTTGCC CGTTTGGGGA AAGTTCCACC TGCTATGGTT 
AACCCACCTG TGGTGAACCC AGCCGGCCGC AGGAGACGCC CCACCAAGAG CAAAAGCAGC 
AAATCCAGCC GCGGGAGCTC GCAGGCTAGC AAGAGTCCCG TACAGCCAGC AACCAGCACT 
TTATCAGCAC AGAGCGCCCC TTCTGTGTAC CCAGCCCAGC AGGCCTTCCT GGCCCCCGGG 
GGGATGATGG ATGGGGGGAG CAGCCCTCTG CTGCAGTCTT TCAAACCCTC GCCCTCCAAT 
GACAACCTGT GCTCCACTTT CACCAGTGAC GCCACGCTCT CTGCGCCCAG TCTCTGTGTA 
ACCTCGCAAG GCTGTGTAAA GTTCAGCTGT GGCTCGGAGA GAGTGACCTT CAAACCAGGT 
GGCAGGAGGA CGCGTTTTCT GAGTACGCCC TGCTTGGCTC TTTGGAAGAT GGTGAAAAAA 
GTCTGCCCCT GCAACCAACT CTGTAGGACG AGCAGCACCA ACACGGTGGC AGGACCCGGT 
CAGAACCAAA GCCAGCCACC CGTCTCTCAG TCTCAGAGCT GCAAGGGCAT GTTCACAGAC 
GAGCTGCACA AACTGGTGGA CAACTGGGCC CGGGACGCCA TGAACCTATC ACAGGGCAAG 
AGAGGCTCCA AACACCAACA GCAAGTGGTT CCTCAGGGCC ACAGCTACGA AATGGTTCCT 
CCAGTAAACA TGGGCCGTAA GTACTCAGCT CCGGGTCAGC TGTGCCCAAG CATCGCTTCA 
ACTCTGAGCG GCCATCCACC AATGCCCAAC ACTCCCGCTA CCTCTCTAGG AGCTCGGAAG 
GGCTCCTTGT GTCCCACGCC GCAGTACGGC TACCCCTCCG CACCTTACAG CGCCCAGTGG 
GCTGGAGCGG CCACGCACAC CCAGGCCGGT CTGCTGGCCA CCTCACAACC CCTGGGCCAA 
TATCCCCAGG TGCCCACCCC TTTACAGACC TTCCACATCA GCACTTTGCA AAAGTCAGTC 
AGCCACCCGG GCGGACCCAA TCTGAAGAGC ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300088.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298363.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

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CloneID	ODa43446
Clone ID Related Accession (Same CDS sequence)	XM_009300088.3 , XM_009300088.2
Accession Version	XM_009300088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298363.1
CDS	1100..8428
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300088.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300088.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300088.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298363.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

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CloneID	ODa43446
Clone ID Related Accession (Same CDS sequence)	XM_009300088.3 , XM_009300088.2
Accession Version	XM_009300088.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298363.1
CDS	1163..8491
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300088.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300088.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGCTG TGTAAAGTTC 
AGCTGTGGCT CGGAGAGAGT GACCTTCAAA CCAGGTGGCA GGAGGACGCG TTTTCTGAGG 
ACGAGCAGCA CCAACACGGT GGCAGGACCC GGTCAGAACC AAAGCCAGCC ACCCGTCTCT 
CAGTCTCAGA GCTGCAAGGG CATGTTCACA GACGAGCTGC ACAAACTGGT GGACAACTGG 
GCCCGGGACG CCATGAACCT ATCACAGGGC AAGAGAGGCT CCAAACACCA ACAGCAAGTG 
GTTCCTCAGG GCCACAGCTA CGAAATGGTT CCTCCAGTAA ACATGGGCCG TAAGTACTCA 
GCTCCGGGTC AGCTGTGCCC AAGCATCGCT TCAACTCTGA GCGGCCATCC ACCAATGCCC 
AACACTCCCG CTACCTCTCT AGGAGCTCGG AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC 
GGCTACCCCT CCGCACCTTA CAGCGCCCAG TGGGCTGGAG CGGCCACGCA CACCCAGGCC 
GGTCTGCTGG CCACCTCACA ACCCCTGGGC CAATATCCCC AGGTGCCCAC CCCTTTACAG 
ACCTTCCACA TCAGCACTTT GCAAAAGTCA GTCAGCCACC CGGGCGGACC CAATCTGAAG 
AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300089.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298364.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

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CloneID	ODa43447
Clone ID Related Accession (Same CDS sequence)	XM_009300089.3 , XM_009300089.2
Accession Version	XM_009300089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTCA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TTAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCTTCTG TGTACCCAGC CCAGCAGGCC 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298364.1
CDS	1100..8410
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300089.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300089.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTCA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TTAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCTTCTG TGTACCCAGC CCAGCAGGCC 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300089.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298364.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

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CloneID	ODa43447
Clone ID Related Accession (Same CDS sequence)	XM_009300089.3 , XM_009300089.2
Accession Version	XM_009300089.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTCA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TTAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCTTCTG TGTACCCAGC CCAGCAGGCC 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298364.1
CDS	1164..8474
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300089.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300089.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGTCC CAGCAGCCCC TGGAGGATCT GGATGCCCAG 
CTGAGACGGG CACTGAGTCC GGAGACCGTC CCAATCAGCA GTGTCACGCA CCAGTCATCT 
CTAACCGGGA TGCCACCTGG AGGACAGCCA GTTCCGTTTT CTCTGGATGA TGAGCAAGCA 
GCCGCACCTG CAGCTGGAGG ATACAAACTG GGGCGATTCC AGGTGTCGGT GGCCACTGAT 
GAGAACACCA CACAGACTCC AACCAGCTCA TCCTCTTCCA CATCATCTTC CTCCACCACC 
TCCTCTTCCT CCTCCTCCTG TAGTCTGTCC AGCCCTGAAA ACACACTGCA CAGAACCTCC 
TCTCCCCTCA TAGACACAGG CGCAGATGTG GTCGATGGTC TCCCTGCTCT CCCCAACCAG 
ACAACGACCC AGCCTCAGCC CACCACCATC GGCCGCTTCC AGGTCACGAC CAAAACAGAC 
ACGCAGGTGG GACGCTTCTC TGTTAGCAAA GCACAGGACG AGGTGACTAC ATCCACCTCA 
CAACCTCAGC CCACACCTCA AACCCATTCT GCTGTGGGCA ACGGTCCAGC ACAGAGTCCC 
GGGAGCTTAA ACAACTCCAT CAGCTCCTAT TTCAGCAGCG ATAATGACTC GGAGTTTGAA 
GACGAGGACT TTAAGAGAGA AGTCAGCAGG CTGAGAGACA AGCACATGAT AGAGAGTCAG 
GCTCTATATT CTCGTCAGAA AGCAGAGATT GAGGCTCTGT TTGCCCGTTT GGGGAAAGTT 
CCACCTGCTA TGGTTAACCC ACCTGTGGTG AACCCAGCCG GCCGCAGGAG ACGCCCCACC 
AAGAGCAAAA GCAGCAAATC CAGCCGCGGG AGCTCGCAGG CTAGCAAGAG TCCCGTACAG 
CCAGCAACCA GCACTTTATC AGCACAGAGC GCCCCTTCTG TGTACCCAGC CCAGCAGGCC 
TTCCTGGCCC CCGGGGGGAT GATGGATGGG GGGAGCAGCC CTCTGCTGCA GTCTTTCAAA 
CCCTCGCCCT CCAATGACAA CCTGTGCTCC ACTTTCACCA GTGACGCCAC GCTCTCTGCG 
CCCAGTCTCT GTGTAACCTC GCAAGGCTGT GTAAAGTTCA GCTGTGGCTC GGAGAGAGTG 
ACCTTCAAAC CAGGTGGCAG GAGGACGCGT TTTCTGAGTA CGCCCTGCTT GGCTCTTTGG 
AAGATGGTGA AAAAAGTCTG CCCCTGCAAC CAACTCTGTA GGACGAGCAG CACCAACACG 
GTGGCAGGAC CCGGTCAGAA CCAAAGCCAG CCACCCGTCT CTCAGTCTCA GAGCTGCAAG 
GGCATGTTCA CAGACGAGCT GCACAAACTG GTGGACAACT GGGCCCGGGA CGCCATGAAC 
CTATCACAGG GCAAGAGAGG CTCCAAACAC CAACAGCAAG TGGTTCCTCA GGGCCACAGC 
TACGAAATGG TTCCTCCAGT AAACATGGGC CGTAAGTACT CAGCTCCGGG TCAGCTGTGC 
CCAAGCATCG CTTCAACTCT GAGCGGCCAT CCACCAATGC CCAACACTCC CGCTACCTCT 
CTAGGAGCTC GGAAGGGCTC CTTGTGTCCC ACGCCGCAGT ACGGCTACCC CTCCGCACCT 
TACAGCGCCC AGTGGGCTGG AGCGGCCACG CACACCCAGG CCGGTCTGCT GGCCACCTCA 
CAACCCCTGG GCCAATATCC CCAGGTGCCC ACCCCTTTAC AGACCTTCCA CATCAGCACT 
TTGCAAAAGT CAGTCAGCCA CCCGGGCGGA CCCAATCTGA AGAGCACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300090.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298365.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

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CloneID	ODa43448
Clone ID Related Accession (Same CDS sequence)	XM_009300090.2
Accession Version	XM_009300090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAA GATGGTGAAA 
AAAGTCTGCC CCTGCAACCA ACTCTGTAGG ACGAGCAGCA CCAACACGGT GGCAGGACCC 
GGTCAGAACC AAAGCCAGCC ACCCGTCTCT CAGTCTCAGA GCTGCAAGGG CATGTTCACA 
GACGAGCTGC ACAAACTGGT GGACAACTGG GCCCGGGACG CCATGAACCT ATCACAGGGC 
AAGAGAGGCT CCAAACACCA ACAGCAAGTG GTTCCTCAGG GCCACAGCTA CGAAATGGTT 
CCTCCAGTAA ACATGGGCCG TAAGTACTCA GCTCCGGGTC AGCTGTGCCC AAGCATCGCT 
TCAACTCTGA GCGGCCATCC ACCAATGCCC AACACTCCCG CTACCTCTCT AGGAGCTCGG 
AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC GGCTACCCCT CCGCACCTTA CAGCGCCCAG 
TGGGCTGGAG CGGCCACGCA CACCCAGGCC GGTCTGCTGG CCACCTCACA ACCCCTGGGC 
CAATATCCCC AGGTGCCCAC CCCTTTACAG ACCTTCCACA TCAGCACTTT GCAAAAGTCA 
GTCAGCCACC CGGGCGGACC CAATCTGAAG AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298365.1
CDS	1163..8461
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300090.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300090.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAA GATGGTGAAA 
AAAGTCTGCC CCTGCAACCA ACTCTGTAGG ACGAGCAGCA CCAACACGGT GGCAGGACCC 
GGTCAGAACC AAAGCCAGCC ACCCGTCTCT CAGTCTCAGA GCTGCAAGGG CATGTTCACA 
GACGAGCTGC ACAAACTGGT GGACAACTGG GCCCGGGACG CCATGAACCT ATCACAGGGC 
AAGAGAGGCT CCAAACACCA ACAGCAAGTG GTTCCTCAGG GCCACAGCTA CGAAATGGTT 
CCTCCAGTAA ACATGGGCCG TAAGTACTCA GCTCCGGGTC AGCTGTGCCC AAGCATCGCT 
TCAACTCTGA GCGGCCATCC ACCAATGCCC AACACTCCCG CTACCTCTCT AGGAGCTCGG 
AAGGGCTCCT TGTGTCCCAC GCCGCAGTAC GGCTACCCCT CCGCACCTTA CAGCGCCCAG 
TGGGCTGGAG CGGCCACGCA CACCCAGGCC GGTCTGCTGG CCACCTCACA ACCCCTGGGC 
CAATATCCCC AGGTGCCCAC CCCTTTACAG ACCTTCCACA TCAGCACTTT GCAAAAGTCA 
GTCAGCCACC CGGGCGGACC CAATCTGAAG AGCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300091.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298366.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

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CloneID	ODa43449
Clone ID Related Accession (Same CDS sequence)	XM_009300091.3 , XM_009300091.2
Accession Version	XM_009300091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAACCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298366.1
CDS	1100..8356
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300091.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300091.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAACCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300091.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298366.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

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CloneID	ODa43449
Clone ID Related Accession (Same CDS sequence)	XM_009300091.3 , XM_009300091.2
Accession Version	XM_009300091.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAACCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298366.1
CDS	1163..8419
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300091.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300091.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGCTTTGCA GTCCGGCTCT CTGCGTTCCA 
ACTATAGCAG TCACCCCGTT TCAGCAGCAA CCTGTGCCTG AAGCGCTGAC GTCCCTGAAA 
ACTGCGCCAG CCACCTCCCC TTCAATGCTG CCGCCCCATG TACCCCTATT GGTAACAACA 
CCTGTGCCAA CCATCCATGT GGCCACAGTC CCTTCCCCCA CCGAACATCC GTGTTTGCCA 
GCAGACGCCT TGGCGCAGAC GTCTCACAGC GCATCGCAGA CTTTGACAAC AGAGCTGTCA 
CAACTCCAAA CTCCTGCTGT GGAGAGCAGT CATTCTGATG TAGCTTCTGG GCTCAGTGAT 
GGCAATGAGG GCGTAACAGG TGGTAGACAT GAGGGTCGTC CTGCCAAAAG ACACCAGCGC 
AGATCGGTGC GCAGCCGTTC ACGCAATGAA AAGACCTCGA AGGTCAAGCT CAGTGTGCTT 
AATATCTCCA GCATGGGTGA CAGAGTTGCA GAGTGTCAAT TAGAAACACA CAACAGGAAA 
ATGGTGACTT TCAAGTTCGA TCTTGATGGT GATAACCCAG AGGAAATTGC ACAGATAATG 
GTACAAAGTG AATTCATTCT TGAAAGCGAG CGTGAATCGT TCATCGATCG GATCCGAGAA 
GTGATCGAAA CAGCTGATGA GAAGGGGGTG GAGAGAGAGC GCAGTCAGAT GGCCAGTGAT 
CATGAGCAGC TTATGCCCAC AACATCTGCG CCTCTTGAAC ATGGTAGAAC TTACAATCTC 
AGTGTACCAC CTAGTTCAGC TGCACAGGTG GTTCATTCTG CAGGCCGCAG GTTTATCGTG 
AGTCCAGTGC CTGAATCCAG ACTGAAGGAT CAGTTCTTTA GCCCATCCCA ACCTACAATC 
CCACTTCATG AAGAACCTGC CCCTGTGCCA GTTGCATTGC CTGCCTTGGG GCTCTCTGTG 
TCTGCGTCTG CTGTCAGCCT GCAGCAGGCG TTCACTGAGA TGCGTCAAGC CCACTTTGAC 
CCAGGTCCCA GCACTGCACC CCCTATGCTC CACAACTCCA TGCCCCCTCT GCTTCCTGCC 
ACAACACCCT TGCCCTCTAG TGCCACCCCA GTGGTCTCTC CCCCAGTGTC TTCAGAAGTG 
ACCTCTTCGC CATCTCTACC TTCTATAAAC CCTCCAGTGT CATCATCTCC TCCTCCAACC 
GTCCCGGTCA ATTCCGTAAC CTCCCCTCCA GCACAACTCA CCCTGCCTCA AAGTCAAGTT 
GCGCCTGCCG TCATCAGTGT TATTCCCACC CCTTCCACTC TGCCAATGCC AGCGGTGCCA 
ACTGCCACAT CCCTTCCACA AACTTCAGTC ACCGTACCTT CCAGCACACC AATCGCTCCA 
ATGGGATCAG TGTCCGGAGG CAGTGGAGAA CAACCATCCA TCCCTGCTAC TTCTTCGCCC 
ACTAGCGCTA CAATCCAAAC GCCACAGCTG GCACCATCCA CCATCCCCAC CACCACGGCT 
CAGTCACAAG TGCCCCCTCT CCAGCCGGTG ACCACAACCA TGCCAGTCGT GCAGCCCACA 
CCAGTGCACA GCCAACCACA GACCACCACC GTGCCCAATC AGAGCCATGC GCATAGTGTT 
GAATGCGACA GTGACCAGCA GGGCAAGGTT GATGACATCC AGGCTCTAGA TAAGAAGCTG 
CGCTCGCTCT TTATGGACTT GGGCTCCGGT CCCCCATCTG CCCAGTCTGA TGTCACATCC 
GACCCGACGG CGACTCCAAG CGTTCCTGGC ACCTCGTCTC CAACCACATG TGCCACCCCC 
AGCGGGACTC CACTCCCTCC CTCTAGTTTA CCCCTGAACT CCTCCACTCA GTCTGCGGGG 
TCTCCCATGA CCTCCATGGG CAATGCCTCC ACCCCGGTCG GCTACAGTCA GACCACTCCA 
TCCAAAGCTC CCTTAACACG ATTACCGGTG TTGCCTGTCG GCCCAGATCA GGCTGGCACG 
CCGCCCACAG AGCACCTTCC ACCCTTCCCA GGACCTGGTT TAATTCAGTC CCAGCAGCCC 
CTGGAGGATC TGGATGCCCA GCTGAGACGG GCACTGAGTC CGGAGACCGT CCCAATCAGC 
AGTGTCACGC ACCAGTCATC TCTAACCGGG ATGCCACCTG GAGGACAGCC AGTTCCGTTT 
TCTCTGGATG ATGAGCAAGC AGCCGCACCT GCAGCTGGAG GATACAAACT GGGGCGATTC 
CAGGTGTCGG TGGCCACTGA TGAGAACACC ACACAGACTC CAACCAGCTC ATCCTCTTCC 
ACATCATCTT CCTCCACCAC CTCCTCTTCC TCCTCCTCCT GTAGTCTGTC CAGCCCTGAA 
AACACACTGC ACAGAACCTC CTCTCCCCTC ATAGACACAG GCGCAGATGT GGTCGATGGT 
CTCCCTGCTC TCCCCAACCA GACAACGACC CAGCCTCAGC CCACCACCAT CGGCCGCTTC 
CAGGTCACGA CCAAAACAGA CACGCAGGTG GGACGCTTCT CTGTTAGCAA AGCACAGGAC 
GAGGTGACTA CATCCACCTC ACAACCTCAG CCCACACCTC AAACCCATTC TGCTGTGGGC 
AACGGTCCAG CACAGAGTCC CGGGAGCTTA AACAACTCCA TCAGCTCCTA TTTCAGCAGC 
GATAATGACT CGGAGTTTGA AGACGAGGAC TTTAAGAGAG AAGTCAGCAG GCTGAGAGAC 
AAGCACATGA TAGAGAGTCA GGCTCTATAT TCTCGTCAGA AAGCAGAGAT TGAGGCTCTG 
TTTGCCCGTT TGGGGAAAGT TCCACCTGCT ATGGTTAACC CACCTGTGGT GAACCCAGCC 
GGCCGCAGGA GACGCCCCAC CAAGAGCAAA AGCAGCAAAT CCAGCCGCGG GAGCTCGCAG 
GCTAGCAAGA GTCCCGTACA GCCAGCAACC AGCACTTTAT CAGCACAGAG CGCCCCTTCT 
GTGTACCCAG CCCAGCAGGC CTTCCTGGCC CCCGGGGGGA TGATGGATGG GGGGAGCAGC 
CCTCTGCTGC AGTCTTTCAA ACCCTCGCCC TCCAATGACA ACCTGTGCTC CACTTTCACC 
AGTGACGCCA CGCTCTCTGC GCCCAGTCTC TGTGTAACCT CGCAAGGGAC GAGCAGCACC 
AACACGGTGG CAGGACCCGG TCAGAACCAA AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC 
TGCAAGGGCA TGTTCACAGA CGAGCTGCAC AAACTGGTGG ACAACTGGGC CCGGGACGCC 
ATGAACCTAT CACAGGGCAA GAGAGGCTCC AAACACCAAC AGCAAGTGGT TCCTCAGGGC 
CACAGCTACG AAATGGTTCC TCCAGTAAAC ATGGGCCGTA AGTACTCAGC TCCGGGTCAG 
CTGTGCCCAA GCATCGCTTC AACTCTGAGC GGCCATCCAC CAATGCCCAA CACTCCCGCT 
ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG TGTCCCACGC CGCAGTACGG CTACCCCTCC 
GCACCTTACA GCGCCCAGTG GGCTGGAGCG GCCACGCACA CCCAGGCCGG TCTGCTGGCC 
ACCTCACAAC CCCTGGGCCA ATATCCCCAG GTGCCCACCC CTTTACAGAC CTTCCACATC 
AGCACTTTGC AAAAGTCAGT CAGCCACCCG GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300092.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298367.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

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CloneID	ODa43450
Clone ID Related Accession (Same CDS sequence)	XM_009300092.3 , XM_009300092.2
Accession Version	XM_009300092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGTGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGT 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACATGGT 
AGAACTTACA ATCTCAGTGT ACCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCAGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCCTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CGGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTCACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTTAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CTTCTGTGTA CCCAGCCCAG CAGGCCTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAACCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298367.1
CDS	1100..8266
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300092.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300092.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGTGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGT 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACATGGT 
AGAACTTACA ATCTCAGTGT ACCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCAGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCCTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CGGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTCACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTTAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CTTCTGTGTA CCCAGCCCAG CAGGCCTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAACCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300092.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298367.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

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CloneID	ODa43450
Clone ID Related Accession (Same CDS sequence)	XM_009300092.3 , XM_009300092.2
Accession Version	XM_009300092.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGTGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGT 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACATGGT 
AGAACTTACA ATCTCAGTGT ACCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCAGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCCTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CGGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTCACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTTAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CTTCTGTGTA CCCAGCCCAG CAGGCCTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAACCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298367.1
CDS	1163..8329
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300092.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300092.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GCAGTCATTC TGATGTAGCT 
TCTGGGCTCA GTGATGGCAA TGAGGGCGTA ACAGGTGGTA GACATGAGGG TCGTCCTGCC 
AAAAGACACC AGCGCAGATC GGTGCGCAGC CGTTCACGCA ATGAAAAGAC CTCGAAGGTC 
AAGCTCAGTG TGCTTAATAT CTCCAGCATG GGTGACAGAG TTGCAGAGTG TCAATTAGAA 
ACACACAACA GGAAAATGGT GACTTTCAAG TTCGATCTTG ATGGTGATAA CCCAGAGGAA 
ATTGCACAGA TAATGGTACA AAGTGAATTC ATTCTTGAAA GCGAGCGTGA ATCGTTCATC 
GATCGGATCC GAGAAGTGAT CGAAACAGCT GATGAGAAGG GGGTGGAGAG AGAGCGCAGT 
CAGATGGCCA GTGATCATGA GCAGCTTATG CCCACAACAT CTGCGCCTCT TGAACATGGT 
AGAACTTACA ATCTCAGTGT ACCACCTAGT TCAGCTGCAC AGGTGGTTCA TTCTGCAGGC 
CGCAGGTTTA TCGTGAGTCC AGTGCCTGAA TCCAGACTGA AGGATCAGTT CTTTAGCCCA 
TCCCAACCTA CAATCCCACT TCATGAAGAA CCTGCCCCTG TGCCAGTTGC ATTGCCTGCC 
TTGGGGCTCT CTGTGTCTGC GTCTGCTGTC AGCCTGCAGC AGGCGTTCAC TGAGATGCGT 
CAAGCCCACT TTGACCCAGG TCCCAGCACT GCACCCCCTA TGCTCCACAA CTCCATGCCC 
CCTCTGCTTC CTGCCACAAC ACCCTTGCCC TCTAGTGCCA CCCCAGTGGT CTCTCCCCCA 
GTGTCTTCAG AAGTGACCTC TTCGCCATCT CTACCTTCTA TAAACCCTCC AGTGTCATCA 
TCTCCTCCTC CAACCGTCCC GGTCAATTCC GTAACCTCCC CTCCAGCACA ACTCACCCTG 
CCTCAAAGTC AAGTTGCGCC TGCCGTCATC AGTGTTATTC CCACCCCTTC CACTCTGCCA 
ATGCCAGCGG TGCCAACTGC CACATCCCTT CCACAAACTT CAGTCACCGT ACCTTCCAGC 
ACACCAATCG CTCCAATGGG ATCAGTGTCC GGAGGCAGTG GAGAACAACC ATCCATCCCT 
GCTACTTCTT CGCCCACTAG CGCTACAATC CAAACGCCAC AGCTGGCACC ATCCACCATC 
CCCACCACCA CGGCTCAGTC ACAAGTGCCC CCTCTCCAGC CGGTGACCAC AACCATGCCA 
GTCGTGCAGC CCACACCAGT GCACAGCCAA CCACAGACCA CCACCGTGCC CAATCAGAGC 
CATGCGCATA GTGTTGAATG CGACAGTGAC CAGCAGGGCA AGGTTGATGA CATCCAGGCT 
CTAGATAAGA AGCTGCGCTC GCTCTTTATG GACTTGGGCT CCGGTCCCCC ATCTGCCCAG 
TCTGATGTCA CATCCGACCC GACGGCGACT CCAAGCGTTC CTGGCACCTC GTCTCCAACC 
ACATGTGCCA CCCCCAGCGG GACTCCACTC CCTCCCTCTA GTTTACCCCT GAACTCCTCC 
ACTCAGTCTG CGGGGTCTCC CATGACCTCC ATGGGCAATG CCTCCACCCC GGTCGGCTAC 
AGTCAGACCA CTCCATCCAA AGCTCCCTTA ACACGATTAC CGGTGTTGCC TGTCGGCCCA 
GATCAGGCTG GCACGCCGCC CACAGAGCAC CTTCCACCCT TCCCAGGACC TGGTTTAATT 
CAGTCCCAGC AGCCCCTGGA GGATCTGGAT GCCCAGCTGA GACGGGCACT GAGTCCGGAG 
ACCGTCCCAA TCAGCAGTGT CACGCACCAG TCATCTCTAA CCGGGATGCC ACCTGGAGGA 
CAGCCAGTTC CGTTTTCTCT GGATGATGAG CAAGCAGCCG CACCTGCAGC TGGAGGATAC 
AAACTGGGGC GATTCCAGGT GTCGGTGGCC ACTGATGAGA ACACCACACA GACTCCAACC 
AGCTCATCCT CTTCCACATC ATCTTCCTCC ACCACCTCCT CTTCCTCCTC CTCCTGTAGT 
CTGTCCAGCC CTGAAAACAC ACTGCACAGA ACCTCCTCTC CCCTCATAGA CACAGGCGCA 
GATGTGGTCG ATGGTCTCCC TGCTCTCCCC AACCAGACAA CGACCCAGCC TCAGCCCACC 
ACCATCGGCC GCTTCCAGGT CACGACCAAA ACAGACACGC AGGTGGGACG CTTCTCTGTT 
AGCAAAGCAC AGGACGAGGT GACTACATCC ACCTCACAAC CTCAGCCCAC ACCTCAAACC 
CATTCTGCTG TGGGCAACGG TCCAGCACAG AGTCCCGGGA GCTTAAACAA CTCCATCAGC 
TCCTATTTCA GCAGCGATAA TGACTCGGAG TTTGAAGACG AGGACTTTAA GAGAGAAGTC 
AGCAGGCTGA GAGACAAGCA CATGATAGAG AGTCAGGCTC TATATTCTCG TCAGAAAGCA 
GAGATTGAGG CTCTGTTTGC CCGTTTGGGG AAAGTTCCAC CTGCTATGGT TAACCCACCT 
GTGGTGAACC CAGCCGGCCG CAGGAGACGC CCCACCAAGA GCAAAAGCAG CAAATCCAGC 
CGCGGGAGCT CGCAGGCTAG CAAGAGTCCC GTACAGCCAG CAACCAGCAC TTTATCAGCA 
CAGAGCGCCC CTTCTGTGTA CCCAGCCCAG CAGGCCTTCC TGGCCCCCGG GGGGATGATG 
GATGGGGGGA GCAGCCCTCT GCTGCAGTCT TTCAAACCCT CGCCCTCCAA TGACAACCTG 
TGCTCCACTT TCACCAGTGA CGCCACGCTC TCTGCGCCCA GTCTCTGTGT AACCTCGCAA 
GGCTGTGTAA AGTTCAGCTG TGGCTCGGAG AGAGTGACCT TCAAACCAGG TGGCAGGAGG 
ACGCGTTTTC TGAGTACGCC CTGCTTGGCT CTTTGGAAGA TGGTGAAAAA AGTCTGCCCC 
TGCAACCAAC TCTGTAGGAC GAGCAGCACC AACACGGTGG CAGGACCCGG TCAGAACCAA 
AGCCAGCCAC CCGTCTCTCA GTCTCAGAGC TGCAAGGGCA TGTTCACAGA CGAGCTGCAC 
AAACTGGTGG ACAACTGGGC CCGGGACGCC ATGAACCTAT CACAGGGCAA GAGAGGCTCC 
AAACACCAAC AGCAAGTGGT TCCTCAGGGC CACAGCTACG AAATGGTTCC TCCAGTAAAC 
ATGGGCCGTA AGTACTCAGC TCCGGGTCAG CTGTGCCCAA GCATCGCTTC AACTCTGAGC 
GGCCATCCAC CAATGCCCAA CACTCCCGCT ACCTCTCTAG GAGCTCGGAA GGGCTCCTTG 
TGTCCCACGC CGCAGTACGG CTACCCCTCC GCACCTTACA GCGCCCAGTG GGCTGGAGCG 
GCCACGCACA CCCAGGCCGG TCTGCTGGCC ACCTCACAAC CCCTGGGCCA ATATCCCCAG 
GTGCCCACCC CTTTACAGAC CTTCCACATC AGCACTTTGC AAAAGTCAGT CAGCCACCCG 
GGCGGACCCA ATCTGAAGAG CACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300093.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298368.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

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CloneID	ODa43451
Clone ID Related Accession (Same CDS sequence)	XM_009300093.3 , XM_009300093.2
Accession Version	XM_009300093.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGTGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGTCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACATGGTAGA 
ACTTACAATC TCAGTGTACC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CAGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCCTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCGG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TCACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTTAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCTT CTGTGTACCC AGCCCAGCAG GCCTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGC 
GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298368.1
CDS	1100..8263
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300093.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300093.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGTGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGTCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACATGGTAGA 
ACTTACAATC TCAGTGTACC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CAGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCCTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCGG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TCACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTTAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCTT CTGTGTACCC AGCCCAGCAG GCCTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGC 
GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300093.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298368.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

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CloneID	ODa43451
Clone ID Related Accession (Same CDS sequence)	XM_009300093.3 , XM_009300093.2
Accession Version	XM_009300093.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGTGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGTCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACATGGTAGA 
ACTTACAATC TCAGTGTACC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CAGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCCTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCGG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TCACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTTAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCTT CTGTGTACCC AGCCCAGCAG GCCTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGC 
GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298368.1
CDS	1163..8326
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMPQSVSQPYGGGGSGLVEPPLFFPTIPERPVSFSPPPSCPPKAFNVQRRKSTSILEAHTRHFQPAYHRSYGSSLHPFSGVEALGATVEPSMFMMPGMPAQRLADPLHLQPPTESLYGYKDVRAEQEETVRRLSLNQAALMDHLEAMAYSGYPMTAHQLSHISFHQQMQAAAASLAYETQSQQPGPAYLHPHLQSHMRLTHSPIPQLPPMSVPSSASAPQQATVADPSYQSHSFPHSAPPVLAHASEPNTAFEFHMHNLQADPAMLASRLYRARRGSMDLNLEEAGGNPGLCSRLQPVTEELYSYVSPELPLPPGSLLLHGPKELSPEPSSDSMTSSDAGEFHSPPPHQLYQTQECSAAQSIPQAAYYKTDGGSNVSDGHPPSQPTFLFVPHSESSTISSSHINAWARQPSLPPDLTFHEPALSHQGATLVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQTSHSASQTLTTELSQLQTPAVESHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300093.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300093.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGCCG 
CAATCAGTGA GTCAGCCGTA CGGCGGAGGA GGCTCAGGTC TGGTCGAGCC CCCACTCTTT 
TTCCCCACCA TCCCGGAACG GCCCGTCTCA TTCTCCCCTC CACCATCTTG CCCACCTAAA 
GCTTTCAACG TGCAGCGCCG CAAGAGCACC TCCATCCTGG AGGCCCACAC GCGTCACTTC 
CAGCCCGCCT ACCACCGGAG CTATGGTAGC AGCCTGCACC CATTCTCTGG CGTGGAGGCA 
CTTGGTGCCA CAGTCGAACC TTCTATGTTT ATGATGCCCG GGATGCCGGC TCAAAGATTG 
GCGGATCCAC TTCACCTACA GCCTCCAACA GAATCACTAT ATGGATATAA AGATGTAAGA 
GCAGAGCAAG AAGAAACAGT ACGCAGGCTC AGCCTCAACC AGGCTGCATT AATGGACCAT 
CTTGAAGCCA TGGCCTACAG CGGCTACCCT ATGACTGCGC ATCAGCTAAG CCACATCAGC 
TTCCATCAGC AAATGCAAGC CGCTGCTGCT AGCTTAGCCT ATGAGACTCA GTCTCAGCAA 
CCTGGGCCAG CCTACCTACA CCCTCACCTA CAGAGCCACA TGCGCTTGAC CCACAGCCCA 
ATCCCCCAAC TACCTCCAAT GAGTGTCCCT AGCTCTGCTT CTGCACCGCA GCAAGCCACA 
GTTGCAGATC CAAGCTATCA GTCCCACTCG TTTCCCCATT CTGCACCACC TGTGTTGGCC 
CATGCATCAG AGCCGAACAC AGCCTTTGAA TTCCACATGC ACAATCTCCA GGCAGACCCA 
GCCATGTTGG CATCTAGACT CTACCGAGCC CGTCGGGGCT CCATGGACCT TAACCTGGAG 
GAAGCAGGTG GCAACCCGGG ATTGTGTAGC CGACTCCAGC CAGTCACAGA AGAACTGTAT 
AGTTATGTGA GCCCAGAGCT CCCTCTTCCA CCAGGCAGCC TGCTTCTTCA TGGACCTAAA 
GAGCTCAGTC CTGAACCATC AAGTGACTCC ATGACCTCCT CAGATGCGGG GGAGTTTCAC 
TCACCTCCGC CACATCAATT GTACCAAACT CAAGAGTGTT CTGCTGCTCA ATCCATCCCA 
CAAGCAGCTT ACTACAAAAC AGATGGAGGG AGTAATGTCA GCGATGGACA CCCACCCAGT 
CAACCTACGT TTCTGTTCGT TCCTCACTCT GAATCATCCA CAATTTCGTC TTCACACATC 
AATGCCTGGG CCCGTCAACC ATCCCTTCCA CCGGACCTCA CATTCCACGA GCCTGCCTTG 
TCCCATCAGG GCGCTACTTT GGTTCAAGTC CCCATTCCTC AGTCTACAAG TGGCATGTCT 
CTGGCTTCCT GCAGCACCCC AGCCGCCTCT GCCTCCCAAC AGTATGGAGT TTACTACGTC 
CAAGCACTCC CTACGCAGGC TCCTGTTCAG CAGCCGGCCT CTCTAGGTCC CACTTCCCAA 
CCCTGTGCCT CGCTACCCCA ACAACAAACA CCCCAACAGC CTTCCAGCAC TGTAGCTCAG 
AGCATCTCCC AAACGCAAAT CACGCCAACA CAGACGTCTC ACAGCGCATC GCAGACTTTG 
ACAACAGAGC TGTCACAACT CCAAACTCCT GCTGTGGAGA GTCATTCTGA TGTAGCTTCT 
GGGCTCAGTG ATGGCAATGA GGGCGTAACA GGTGGTAGAC ATGAGGGTCG TCCTGCCAAA 
AGACACCAGC GCAGATCGGT GCGCAGCCGT TCACGCAATG AAAAGACCTC GAAGGTCAAG 
CTCAGTGTGC TTAATATCTC CAGCATGGGT GACAGAGTTG CAGAGTGTCA ATTAGAAACA 
CACAACAGGA AAATGGTGAC TTTCAAGTTC GATCTTGATG GTGATAACCC AGAGGAAATT 
GCACAGATAA TGGTACAAAG TGAATTCATT CTTGAAAGCG AGCGTGAATC GTTCATCGAT 
CGGATCCGAG AAGTGATCGA AACAGCTGAT GAGAAGGGGG TGGAGAGAGA GCGCAGTCAG 
ATGGCCAGTG ATCATGAGCA GCTTATGCCC ACAACATCTG CGCCTCTTGA ACATGGTAGA 
ACTTACAATC TCAGTGTACC ACCTAGTTCA GCTGCACAGG TGGTTCATTC TGCAGGCCGC 
AGGTTTATCG TGAGTCCAGT GCCTGAATCC AGACTGAAGG ATCAGTTCTT TAGCCCATCC 
CAACCTACAA TCCCACTTCA TGAAGAACCT GCCCCTGTGC CAGTTGCATT GCCTGCCTTG 
GGGCTCTCTG TGTCTGCGTC TGCTGTCAGC CTGCAGCAGG CGTTCACTGA GATGCGTCAA 
GCCCACTTTG ACCCAGGTCC CAGCACTGCA CCCCCTATGC TCCACAACTC CATGCCCCCT 
CTGCTTCCTG CCACAACACC CTTGCCCTCT AGTGCCACCC CAGTGGTCTC TCCCCCAGTG 
TCTTCAGAAG TGACCTCTTC GCCATCTCTA CCTTCTATAA ACCCTCCAGT GTCATCATCT 
CCTCCTCCAA CCGTCCCGGT CAATTCCGTA ACCTCCCCTC CAGCACAACT CACCCTGCCT 
CAAAGTCAAG TTGCGCCTGC CGTCATCAGT GTTATTCCCA CCCCTTCCAC TCTGCCAATG 
CCAGCGGTGC CAACTGCCAC ATCCCTTCCA CAAACTTCAG TCACCGTACC TTCCAGCACA 
CCAATCGCTC CAATGGGATC AGTGTCCGGA GGCAGTGGAG AACAACCATC CATCCCTGCT 
ACTTCTTCGC CCACTAGCGC TACAATCCAA ACGCCACAGC TGGCACCATC CACCATCCCC 
ACCACCACGG CTCAGTCACA AGTGCCCCCT CTCCAGCCGG TGACCACAAC CATGCCAGTC 
GTGCAGCCCA CACCAGTGCA CAGCCAACCA CAGACCACCA CCGTGCCCAA TCAGAGCCAT 
GCGCATAGTG TTGAATGCGA CAGTGACCAG CAGGGCAAGG TTGATGACAT CCAGGCTCTA 
GATAAGAAGC TGCGCTCGCT CTTTATGGAC TTGGGCTCCG GTCCCCCATC TGCCCAGTCT 
GATGTCACAT CCGACCCGAC GGCGACTCCA AGCGTTCCTG GCACCTCGTC TCCAACCACA 
TGTGCCACCC CCAGCGGGAC TCCACTCCCT CCCTCTAGTT TACCCCTGAA CTCCTCCACT 
CAGTCTGCGG GGTCTCCCAT GACCTCCATG GGCAATGCCT CCACCCCGGT CGGCTACAGT 
CAGACCACTC CATCCAAAGC TCCCTTAACA CGATTACCGG TGTTGCCTGT CGGCCCAGAT 
CAGGCTGGCA CGCCGCCCAC AGAGCACCTT CCACCCTTCC CAGGACCTGG TTTAATTCAG 
TCCCAGCAGC CCCTGGAGGA TCTGGATGCC CAGCTGAGAC GGGCACTGAG TCCGGAGACC 
GTCCCAATCA GCAGTGTCAC GCACCAGTCA TCTCTAACCG GGATGCCACC TGGAGGACAG 
CCAGTTCCGT TTTCTCTGGA TGATGAGCAA GCAGCCGCAC CTGCAGCTGG AGGATACAAA 
CTGGGGCGAT TCCAGGTGTC GGTGGCCACT GATGAGAACA CCACACAGAC TCCAACCAGC 
TCATCCTCTT CCACATCATC TTCCTCCACC ACCTCCTCTT CCTCCTCCTC CTGTAGTCTG 
TCCAGCCCTG AAAACACACT GCACAGAACC TCCTCTCCCC TCATAGACAC AGGCGCAGAT 
GTGGTCGATG GTCTCCCTGC TCTCCCCAAC CAGACAACGA CCCAGCCTCA GCCCACCACC 
ATCGGCCGCT TCCAGGTCAC GACCAAAACA GACACGCAGG TGGGACGCTT CTCTGTTAGC 
AAAGCACAGG ACGAGGTGAC TACATCCACC TCACAACCTC AGCCCACACC TCAAACCCAT 
TCTGCTGTGG GCAACGGTCC AGCACAGAGT CCCGGGAGCT TAAACAACTC CATCAGCTCC 
TATTTCAGCA GCGATAATGA CTCGGAGTTT GAAGACGAGG ACTTTAAGAG AGAAGTCAGC 
AGGCTGAGAG ACAAGCACAT GATAGAGAGT CAGGCTCTAT ATTCTCGTCA GAAAGCAGAG 
ATTGAGGCTC TGTTTGCCCG TTTGGGGAAA GTTCCACCTG CTATGGTTAA CCCACCTGTG 
GTGAACCCAG CCGGCCGCAG GAGACGCCCC ACCAAGAGCA AAAGCAGCAA ATCCAGCCGC 
GGGAGCTCGC AGGCTAGCAA GAGTCCCGTA CAGCCAGCAA CCAGCACTTT ATCAGCACAG 
AGCGCCCCTT CTGTGTACCC AGCCCAGCAG GCCTTCCTGG CCCCCGGGGG GATGATGGAT 
GGGGGGAGCA GCCCTCTGCT GCAGTCTTTC AAACCCTCGC CCTCCAATGA CAACCTGTGC 
TCCACTTTCA CCAGTGACGC CACGCTCTCT GCGCCCAGTC TCTGTGTAAC CTCGCAAGGC 
TGTGTAAAGT TCAGCTGTGG CTCGGAGAGA GTGACCTTCA AACCAGGTGG CAGGAGGACG 
CGTTTTCTGA GTACGCCCTG CTTGGCTCTT TGGAAGATGG TGAAAAAAGT CTGCCCCTGC 
AACCAACTCT GTAGGACGAG CAGCACCAAC ACGGTGGCAG GACCCGGTCA GAACCAAAGC 
CAGCCACCCG TCTCTCAGTC TCAGAGCTGC AAGGGCATGT TCACAGACGA GCTGCACAAA 
CTGGTGGACA ACTGGGCCCG GGACGCCATG AACCTATCAC AGGGCAAGAG AGGCTCCAAA 
CACCAACAGC AAGTGGTTCC TCAGGGCCAC AGCTACGAAA TGGTTCCTCC AGTAAACATG 
GGCCGTAAGT ACTCAGCTCC GGGTCAGCTG TGCCCAAGCA TCGCTTCAAC TCTGAGCGGC 
CATCCACCAA TGCCCAACAC TCCCGCTACC TCTCTAGGAG CTCGGAAGGG CTCCTTGTGT 
CCCACGCCGC AGTACGGCTA CCCCTCCGCA CCTTACAGCG CCCAGTGGGC TGGAGCGGCC 
ACGCACACCC AGGCCGGTCT GCTGGCCACC TCACAACCCC TGGGCCAATA TCCCCAGGTG 
CCCACCCCTT TACAGACCTT CCACATCAGC ACTTTGCAAA AGTCAGTCAG CCACCCGGGC 
GGACCCAATC TGAAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300094.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298369.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.

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CloneID	ODa43452
Clone ID Related Accession (Same CDS sequence)	XM_009300094.3 , XM_009300094.2
Accession Version	XM_009300094.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGCGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGTG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG TCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACATGG TAGAACTTAC AATCTCAGTG TACCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCAGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCCT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCGGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTCACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTTA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCTTCTGTGT ACCCAGCCCA GCAGGCCTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAACCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGCGGACCC AATCTGAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298369.1
CDS	1099..7206
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300094.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300094.3

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGCGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGTG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG TCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACATGG TAGAACTTAC AATCTCAGTG TACCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCAGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCCT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCGGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTCACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTTA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCTTCTGTGT ACCCAGCCCA GCAGGCCTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAACCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGCGGACCC AATCTGAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1b ( XM_009300094.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298369.1 Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

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CloneID	ODa43452
Clone ID Related Accession (Same CDS sequence)	XM_009300094.3 , XM_009300094.2
Accession Version	XM_009300094.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGCGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGTG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG TCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACATGG TAGAACTTAC AATCTCAGTG TACCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCAGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCCT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCGGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTCACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTTA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCTTCTGTGT ACCCAGCCCA GCAGGCCTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAACCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGCGGACCC AATCTGAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298369.1
CDS	1161..7268
Translation	MSSENPNKVVTFLAPPPPKNVNGSGSDSLVGEKLDTEVRKRRHTMDKDLKTAEHRFFRRSVICDSNATALDLPSKACILTSPPDCEQFVIPTVLSSTPDVTLDTQTVVSAAVVEERSEDERRAGVEGIVEVQSPSVQDAGSRQSEVLGKVETELSDVGDKEQESRVTDKQEEEERGVAKARAEAELREAVKKEQDEIEEVETKAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSRSERQRFKEEAGMLKGLQHPNIVRFYDSWESPSKGRKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLHFLHTRAPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRSSFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNPAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYAIKDLLNHAFFQEETGVRVELAEEDDGEMIAIKLWLRIEDVKKLKGKYKDNEAIEFSFDLHRDVPDDVAQEMVESGYVCEGDHKTMAKAIRDRVSLISRKREQRQQVRAEQEKRKQEEEQKLLSSESIKNVAGAQGTQGSLQSQCSSQPPTPGLVQPECEEPDADPQHQYMQSGITLADGTFDSGQGSSVFSEPHLSQLSMSYSSPGTSQQQQVPGQGTYTPSSQAPQQHTAYPQQPMVQVPIPQSTSGMSLASCSTPAASASQQYGVYYVQALPTQAPVQQPASLGPTSQPCASLPQQQTPQQPSSTVAQSISQTQITPTQLCSPALCVPTIAVTPFQQQPVPEALTSLKTAPATSPSMLPPHVPLLVTTPVPTIHVATVPSPTEHPCLPADALAQTSHSASQTLTTELSQLQTPAVESSHSDVASGLSDGNEGVTGGRHEGRPAKRHQRRSVRSRSRNEKTSKVKLSVLNISSMGDRVAECQLETHNRKMVTFKFDLDGDNPEEIAQIMVQSEFILESERESFIDRIREVIETADEKGVERERSQMASDHEQLMPTTSAPLEHGRTYNLSVPPSSAAQVVHSAGRRFIVSPVPESRLKDQFFSPSQPTIPLHEEPAPVPVALPALGLSVSASAVSLQQAFTEMRQAHFDPGPSTAPPMLHNSMPPLLPATTPLPSSATPVVSPPVSSEVTSSPSLPSINPPVSSSPPPTVPVNSVTSPPAQLTLPQSQVAPAVISVIPTPSTLPMPAVPTATSLPQTSVTVPSSTPIAPMGSVSGGSGEQPSIPATSSPTSATIQTPQLAPSTIPTTTAQSQVPPLQPVTTTMPVVQPTPVHSQPQTTTVPNQSHAHSVECDSDQQGKVDDIQALDKKLRSLFMDLGSGPPSAQSDVTSDPTATPSVPGTSSPTTCATPSGTPLPPSSLPLNSSTQSAGSPMTSMGNASTPVGYSQTTPSKAPLTRLPVLPVGPDQAGTPPTEHLPPFPGPGLIQSQQPLEDLDAQLRRALSPETVPISSVTHQSSLTGMPPGGQPVPFSLDDEQAAAPAAGGYKLGRFQVSVATDENTTQTPTSSSSSTSSSSTTSSSSSSCSLSSPENTLHRTSSPLIDTGADVVDGLPALPNQTTTQPQPTTIGRFQVTTKTDTQVGRFSVSKAQDEVTTSTSQPQPTPQTHSAVGNGPAQSPGSLNNSISSYFSSDNDSEFEDEDFKREVSRLRDKHMIESQALYSRQKAEIEALFARLGKVPPAMVNPPVVNPAGRRRRPTKSKSSKSSRGSSQASKSPVQPATSTLSAQSAPSVYPAQQAFLAPGGMMDGGSSPLLQSFKPSPSNDNLCSTFTSDATLSAPSLCVTSQGCVKFSCGSERVTFKPGGRRTRFLSTPCLALWKMVKKVCPCNQLCRTSSTNTVAGPGQNQSQPPVSQSQSCKGMFTDELHKLVDNWARDAMNLSQGKRGSKHQQQVVPQGHSYEMVPPVNMGRKYSAPGQLCPSIASTLSGHPPMPNTPATSLGARKGSLCPTPQYGYPSAPYSAQWAGAATHTQAGLLATSQPLGQYPQVPTPLQTFHISTLQKSVSHPGGPNLKST

Target ORF information:

RefSeq Version	XM_009300094.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1b (wnk1b), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300094.2

ORF Insert Sequence:

ATGTCATCGG AAAATCCCAA CAAGGTGGTG ACCTTCTTGG CCCCGCCACC TCCAAAGAAT 
GTGAATGGCT CCGGTTCAGA CTCGCTGGTT GGTGAGAAGC TAGACACGGA GGTCAGAAAG 
CGACGCCACA CCATGGATAA AGATCTGAAG ACGGCCGAGC ATAGATTTTT CAGACGTAGC 
GTCATCTGTG ACTCCAATGC CACAGCCCTT GACTTGCCCA GCAAGGCCTG CATCCTCACC 
TCACCTCCAG ACTGCGAGCA GTTTGTTATA CCCACGGTGC TGAGTTCGAC CCCTGATGTA 
ACTCTTGACA CCCAAACAGT AGTTTCGGCG GCCGTAGTGG AAGAACGTTC AGAGGATGAA 
AGACGTGCTG GGGTTGAAGG GATTGTGGAG GTACAGAGTC CTTCTGTGCA GGATGCAGGC 
TCAAGGCAGA GTGAAGTCCT TGGGAAGGTG GAGACCGAGC TCAGTGATGT TGGTGACAAA 
GAGCAAGAGA GTCGAGTGAC AGACAAGCAG GAGGAGGAGG AGAGAGGGGT GGCGAAGGCC 
CGTGCTGAAG CTGAGCTGAG GGAGGCTGTG AAAAAGGAGC AGGACGAAAT TGAAGAAGTC 
GAGACCAAAG CTGTGGGCAC CTCTCCTGAT GGCCGCTTCC TCAAATTTGA CATCGAAATT 
GGACGTGGAT CATTCAAAAC GGTCTACAAG GGCTTGGACA CAGAGACAAC AGTGGAGGTG 
GCGTGGTGTG AACTTCAGGA TCGGAAGCTG TCCAGATCAG AGCGCCAGAG ATTCAAGGAA 
GAGGCCGGCA TGCTGAAAGG CCTGCAGCAC CCAAACATTG TGCGTTTTTA CGATTCATGG 
GAGTCTCCTT CCAAAGGGAG GAAGTGCATA GTGCTGGTGA CGGAGCTCAT GACATCAGGA 
ACCCTGAAAA CATATCTGAA ACGCTTCAAG GTGATGAAGA TAAAGGTGTT GCGAAGCTGG 
TGCCGACAAA TCCTGAAGGG CCTGCACTTC CTGCACACCA GGGCTCCACC AATCATCCAC 
CGGGACCTGA AGTGTGACAA CATCTTCATC ACCGGACCCA CCGGCTCAGT AAAGATCGGT 
GATCTGGGCC TGGCCACCCT CAAGCGTTCT TCGTTCGCTA AAAGCGTCAT AGGTACCCCT 
GAGTTCATGG CGCCTGAGAT GTACGAGGAA AAATACGACG AGTCGGTGGA CGTGTACGCC 
TTCGGGATGT GCATGCTGGA GATGGCTACC TCAGAGTACC CGTACTCCGA GTGTCAAAAC 
CCTGCTCAAA TCTATCGCAG AGTCACCAGC GGAGTTAAAC CTGGCAGTTT CGACAAGGTA 
GCCATTCCAG AAGTGAAAGA GATCATCGAG GGATGTATTC GGCAAAACAA AGATGAAAGA 
TACGCCATTA AAGACCTTCT GAACCATGCC TTCTTCCAAG AGGAGACCGG GGTGCGGGTG 
GAACTGGCTG AGGAGGATGA TGGGGAGATG ATCGCCATCA AGCTCTGGTT GCGCATTGAA 
GACGTCAAGA AACTGAAAGG CAAATACAAA GACAACGAAG CCATCGAGTT CTCCTTCGAC 
CTCCATCGGG ACGTGCCAGA CGATGTGGCT CAGGAAATGG TCGAGTCGGG TTATGTTTGC 
GAGGGTGACC ACAAAACCAT GGCCAAGGCC ATCAGGGACC GTGTGTCGCT GATCTCCCGC 
AAACGAGAAC AACGACAACA GGTGCGAGCC GAGCAAGAAA AACGCAAGCA GGAGGAAGAG 
CAGAAACTGT TATCCAGTGA GTCTATTAAG AACGTGGCCG GAGCTCAAGG AACGCAAGGT 
TCTCTACAGA GTCAGTGTAG CTCCCAACCT CCAACTCCCG GCCTTGTGCA GCCCGAGTGT 
GAGGAGCCTG ATGCAGATCC GCAACATCAA TACATGCAAA GTGGAATAAC CTTGGCGGAC 
GGGACATTTG ACAGTGGTCA GGGTTCATCA GTGTTCTCAG AACCTCACCT CAGCCAGCTA 
AGCATGTCGT ATAGTTCTCC TGGAACATCC CAACAACAGC AGGTCCCAGG ACAGGGCACC 
TACACTCCCA GCTCTCAAGC TCCCCAACAG CACACCGCAT ATCCCCAGCA GCCCATGGTT 
CAAGTCCCCA TTCCTCAGTC TACAAGTGGC ATGTCTCTGG CTTCCTGCAG CACCCCAGCC 
GCCTCTGCCT CCCAACAGTA TGGAGTTTAC TACGTCCAAG CACTCCCTAC GCAGGCTCCT 
GTTCAGCAGC CGGCCTCTCT AGGTCCCACT TCCCAACCCT GTGCCTCGCT ACCCCAACAA 
CAAACACCCC AACAGCCTTC CAGCACTGTA GCTCAGAGCA TCTCCCAAAC GCAAATCACG 
CCAACACAGC TTTGCAGTCC GGCTCTCTGC GTTCCAACTA TAGCAGTCAC CCCGTTTCAG 
CAGCAACCTG TGCCTGAAGC GCTGACGTCC CTGAAAACTG CGCCAGCCAC CTCCCCTTCA 
ATGCTGCCGC CCCATGTACC CCTATTGGTA ACAACACCTG TGCCAACCAT CCATGTGGCC 
ACAGTCCCTT CCCCCACCGA ACATCCGTGT TTGCCAGCAG ACGCCTTGGC GCAGACGTCT 
CACAGCGCAT CGCAGACTTT GACAACAGAG CTGTCACAAC TCCAAACTCC TGCTGTGGAG 
AGCAGTCATT CTGATGTAGC TTCTGGGCTC AGTGATGGCA ATGAGGGCGT AACAGGTGGT 
AGACATGAGG GTCGTCCTGC CAAAAGACAC CAGCGCAGAT CGGTGCGCAG CCGTTCACGC 
AATGAAAAGA CCTCGAAGGT CAAGCTCAGT GTGCTTAATA TCTCCAGCAT GGGTGACAGA 
GTTGCAGAGT GTCAATTAGA AACACACAAC AGGAAAATGG TGACTTTCAA GTTCGATCTT 
GATGGTGATA ACCCAGAGGA AATTGCACAG ATAATGGTAC AAAGTGAATT CATTCTTGAA 
AGCGAGCGTG AATCGTTCAT CGATCGGATC CGAGAAGTGA TCGAAACAGC TGATGAGAAG 
GGGGTGGAGA GAGAGCGCAG TCAGATGGCC AGTGATCATG AGCAGCTTAT GCCCACAACA 
TCTGCGCCTC TTGAACATGG TAGAACTTAC AATCTCAGTG TACCACCTAG TTCAGCTGCA 
CAGGTGGTTC ATTCTGCAGG CCGCAGGTTT ATCGTGAGTC CAGTGCCTGA ATCCAGACTG 
AAGGATCAGT TCTTTAGCCC ATCCCAACCT ACAATCCCAC TTCATGAAGA ACCTGCCCCT 
GTGCCAGTTG CATTGCCTGC CTTGGGGCTC TCTGTGTCTG CGTCTGCTGT CAGCCTGCAG 
CAGGCGTTCA CTGAGATGCG TCAAGCCCAC TTTGACCCAG GTCCCAGCAC TGCACCCCCT 
ATGCTCCACA ACTCCATGCC CCCTCTGCTT CCTGCCACAA CACCCTTGCC CTCTAGTGCC 
ACCCCAGTGG TCTCTCCCCC AGTGTCTTCA GAAGTGACCT CTTCGCCATC TCTACCTTCT 
ATAAACCCTC CAGTGTCATC ATCTCCTCCT CCAACCGTCC CGGTCAATTC CGTAACCTCC 
CCTCCAGCAC AACTCACCCT GCCTCAAAGT CAAGTTGCGC CTGCCGTCAT CAGTGTTATT 
CCCACCCCTT CCACTCTGCC AATGCCAGCG GTGCCAACTG CCACATCCCT TCCACAAACT 
TCAGTCACCG TACCTTCCAG CACACCAATC GCTCCAATGG GATCAGTGTC CGGAGGCAGT 
GGAGAACAAC CATCCATCCC TGCTACTTCT TCGCCCACTA GCGCTACAAT CCAAACGCCA 
CAGCTGGCAC CATCCACCAT CCCCACCACC ACGGCTCAGT CACAAGTGCC CCCTCTCCAG 
CCGGTGACCA CAACCATGCC AGTCGTGCAG CCCACACCAG TGCACAGCCA ACCACAGACC 
ACCACCGTGC CCAATCAGAG CCATGCGCAT AGTGTTGAAT GCGACAGTGA CCAGCAGGGC 
AAGGTTGATG ACATCCAGGC TCTAGATAAG AAGCTGCGCT CGCTCTTTAT GGACTTGGGC 
TCCGGTCCCC CATCTGCCCA GTCTGATGTC ACATCCGACC CGACGGCGAC TCCAAGCGTT 
CCTGGCACCT CGTCTCCAAC CACATGTGCC ACCCCCAGCG GGACTCCACT CCCTCCCTCT 
AGTTTACCCC TGAACTCCTC CACTCAGTCT GCGGGGTCTC CCATGACCTC CATGGGCAAT 
GCCTCCACCC CGGTCGGCTA CAGTCAGACC ACTCCATCCA AAGCTCCCTT AACACGATTA 
CCGGTGTTGC CTGTCGGCCC AGATCAGGCT GGCACGCCGC CCACAGAGCA CCTTCCACCC 
TTCCCAGGAC CTGGTTTAAT TCAGTCCCAG CAGCCCCTGG AGGATCTGGA TGCCCAGCTG 
AGACGGGCAC TGAGTCCGGA GACCGTCCCA ATCAGCAGTG TCACGCACCA GTCATCTCTA 
ACCGGGATGC CACCTGGAGG ACAGCCAGTT CCGTTTTCTC TGGATGATGA GCAAGCAGCC 
GCACCTGCAG CTGGAGGATA CAAACTGGGG CGATTCCAGG TGTCGGTGGC CACTGATGAG 
AACACCACAC AGACTCCAAC CAGCTCATCC TCTTCCACAT CATCTTCCTC CACCACCTCC 
TCTTCCTCCT CCTCCTGTAG TCTGTCCAGC CCTGAAAACA CACTGCACAG AACCTCCTCT 
CCCCTCATAG ACACAGGCGC AGATGTGGTC GATGGTCTCC CTGCTCTCCC CAACCAGACA 
ACGACCCAGC CTCAGCCCAC CACCATCGGC CGCTTCCAGG TCACGACCAA AACAGACACG 
CAGGTGGGAC GCTTCTCTGT TAGCAAAGCA CAGGACGAGG TGACTACATC CACCTCACAA 
CCTCAGCCCA CACCTCAAAC CCATTCTGCT GTGGGCAACG GTCCAGCACA GAGTCCCGGG 
AGCTTAAACA ACTCCATCAG CTCCTATTTC AGCAGCGATA ATGACTCGGA GTTTGAAGAC 
GAGGACTTTA AGAGAGAAGT CAGCAGGCTG AGAGACAAGC ACATGATAGA GAGTCAGGCT 
CTATATTCTC GTCAGAAAGC AGAGATTGAG GCTCTGTTTG CCCGTTTGGG GAAAGTTCCA 
CCTGCTATGG TTAACCCACC TGTGGTGAAC CCAGCCGGCC GCAGGAGACG CCCCACCAAG 
AGCAAAAGCA GCAAATCCAG CCGCGGGAGC TCGCAGGCTA GCAAGAGTCC CGTACAGCCA 
GCAACCAGCA CTTTATCAGC ACAGAGCGCC CCTTCTGTGT ACCCAGCCCA GCAGGCCTTC 
CTGGCCCCCG GGGGGATGAT GGATGGGGGG AGCAGCCCTC TGCTGCAGTC TTTCAAACCC 
TCGCCCTCCA ATGACAACCT GTGCTCCACT TTCACCAGTG ACGCCACGCT CTCTGCGCCC 
AGTCTCTGTG TAACCTCGCA AGGCTGTGTA AAGTTCAGCT GTGGCTCGGA GAGAGTGACC 
TTCAAACCAG GTGGCAGGAG GACGCGTTTT CTGAGTACGC CCTGCTTGGC TCTTTGGAAG 
ATGGTGAAAA AAGTCTGCCC CTGCAACCAA CTCTGTAGGA CGAGCAGCAC CAACACGGTG 
GCAGGACCCG GTCAGAACCA AAGCCAGCCA CCCGTCTCTC AGTCTCAGAG CTGCAAGGGC 
ATGTTCACAG ACGAGCTGCA CAAACTGGTG GACAACTGGG CCCGGGACGC CATGAACCTA 
TCACAGGGCA AGAGAGGCTC CAAACACCAA CAGCAAGTGG TTCCTCAGGG CCACAGCTAC 
GAAATGGTTC CTCCAGTAAA CATGGGCCGT AAGTACTCAG CTCCGGGTCA GCTGTGCCCA 
AGCATCGCTT CAACTCTGAG CGGCCATCCA CCAATGCCCA ACACTCCCGC TACCTCTCTA 
GGAGCTCGGA AGGGCTCCTT GTGTCCCACG CCGCAGTACG GCTACCCCTC CGCACCTTAC 
AGCGCCCAGT GGGCTGGAGC GGCCACGCAC ACCCAGGCCG GTCTGCTGGC CACCTCACAA 
CCCCTGGGCC AATATCCCCA GGTGCCCACC CCTTTACAGA CCTTCCACAT CAGCACTTTG 
CAAAAGTCAG TCAGCCACCC GGGCGGACCC AATCTGAAGA GCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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wnk1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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