grk4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	grk4
Entrez Gene ID	560761
Full Name	G protein-coupled receptor kinase 4
Synonyms	zgc:153020
General protein information	Preferred Names G protein-coupled receptor kinase 4 Names G protein-coupled receptor kinase 4 GRK5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001362619.1	NP_001349548.1	G protein-coupled receptor kinase 4 isoform 1
XM_009306441.2	XP_009304716.1	G protein-coupled receptor kinase 4 isoform X1
NM_001079986.1	NP_001073455.1	G protein-coupled receptor kinase 4
NM_001079986.2	NP_001073455.1	G protein-coupled receptor kinase 4 isoform 2
XM_009306444.2	XP_009304719.1	G protein-coupled receptor kinase 4 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRK4	XP_001152424.1
Rattus norvegicus (Norway rat)	Grk4	NP_075217.1
Xenopus tropicalis (tropical clawed frog)	grk4	NP_001006112.1
Canis lupus familiaris (dog)	GRK4	XP_005618794.1
Mus musculus (house mouse)	Grk4	NP_062370.2
Bos taurus (cattle)	GRK4	XP_005208321.1
Gallus gallus (chicken)	GRK4	NP_001006459.1
Danio rerio (zebrafish)	grk4	NP_001073455.1
Homo sapiens (human)	GRK4	NP_892027.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

An essential role for Grk2 in Hedgehog signalling downstream of Smoothened.
Zhao Z, Lee RT, Pusapati GV, Iyu A, Rohatgi R, Ingham PW
EMBO reports17(5)739-52(2016 05)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genome-wide RNA interference in Drosophila cells identifies G protein-coupled receptor kinase 2 as a conserved regulator of NF-kappaB signaling.
Valanne S, Myllym?ki H, Kallio J, Schmid MR, Kleino A, Murum?gi A, Airaksinen L, Kotipelto T, Kaustio M, Ulvila J, Esfahani SS, Engstr?m Y, Silvennoinen O, Hultmark D, Parikka M, R?met M
Journal of immunology (Baltimore, Md. : 1950)184(11)6188-98(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following grk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05485	NM_001362619.1 Latest version!	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa05486	NM_001079986.2 Latest version!	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa31044	NM_001079986.1 Latest version!	Danio rerio G protein-coupled receptor kinase 4 (grk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa05485	XM_009306441.2 Latest version!	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa05486	XM_009306444.2 Latest version!	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grk4 ( NM_001362619.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001349548.1 Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa05485
Clone ID Related Accession (Same CDS sequence)	NM_001362619.1 , XM_009306441.2
Accession Version	NM_001362619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	G protein-coupled receptor kinase 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX908747.25. On Apr 21, 2018 this sequence version replaced XM_009306441.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01011992.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGTTTGT 
TTTGGGAGTG CCAACAGTGA GGAGGAGGAG AAAGAGGAGA AGGAGGAGGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349548.1
CDS	307..2046
Translation	MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREVCFGSANSEEEEKEEKEEEPS

Target ORF information:

RefSeq Version	NM_001362619.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362619.1

ORF Insert Sequence:

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGTTTGT 
TTTGGGAGTG CCAACAGTGA GGAGGAGGAG AAAGAGGAGA AGGAGGAGGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grk4 ( NM_001079986.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073455.1 Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa05486
Clone ID Related Accession (Same CDS sequence)	NM_001079986.2 , XM_009306444.2
Accession Version	NM_001079986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	G protein-coupled receptor kinase 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX908747.25. On or before Apr 21, 2018 this sequence version replaced XM_009306444.2, NM_001079986.1. Transcript Variant: This variant (2) uses an alternate splice junction in the 3' end compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC124213.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073455.1
CDS	307..2028
Translation	MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREASGSASPAVLRGGGA

Target ORF information:

RefSeq Version	NM_001079986.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079986.2

ORF Insert Sequence:

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grk4 ( NM_001079986.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001073455.1 Danio rerio G protein-coupled receptor kinase 4 (grk4), mRNA.

Price & Availability↑

CloneID	ODa31044
Clone ID Related Accession (Same CDS sequence)	NM_001079986.1
Accession Version	NM_001079986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	G protein-coupled receptor kinase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124213.1. On Mar 11, 2007 this sequence version replaced XM_684159.2. ##Evidence-Data-START## Transcript exon combination :: BC124213.1 [ECO:0000332] ##Evidence-Data-END##

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGGC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTTACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCAAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAAGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCACTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073455.1
CDS	268..1989
Translation	MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREASGSASPAVLRGGGA

Target ORF information:

RefSeq Version	NM_001079986.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase 4 (grk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079986.1

ORF Insert Sequence:

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGGC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTTACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCAAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAAGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCACTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grk4 ( XM_009306441.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304716.1 Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05485
Clone ID Related Accession (Same CDS sequence)	NM_001362619.1 , XM_009306441.2
Accession Version	XM_009306441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	G protein-coupled receptor kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGTTTGT 
TTTGGGAGTG CCAACAGTGA GGAGGAGGAG AAAGAGGAGA AGGAGGAGGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304716.1
CDS	302..2041
Translation	MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREVCFGSANSEEEEKEEKEEEPS

Target ORF information:

RefSeq Version	XM_009306441.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306441.2

ORF Insert Sequence:

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGTTTGT 
TTTGGGAGTG CCAACAGTGA GGAGGAGGAG AAAGAGGAGA AGGAGGAGGA GCCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grk4 ( XM_009306444.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304719.1 Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa05486
Clone ID Related Accession (Same CDS sequence)	NM_001079986.2 , XM_009306444.2
Accession Version	XM_009306444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	G protein-coupled receptor kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304719.1
CDS	302..2023
Translation	MEIENIVANTVLLKAREGGGGKRNGRSKKWKEMLKLPHISQCEELRRTIERDYTSLCEKQPIGRQLFRQFCDTQPKLRRCIEFLDSVAMYQVAPDEKRRDAGLNILDTYFNNGSAAHLPEMAQDVVGECRQKLEQSPCKELFQECTRIVREYLSGAPFSAYQETMYFSRFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRVKKRKGEAMALNEKRILEKVNSSFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNSGFDEQRAIFYAAEICCGLEDLHRERIVYRDLKPENILLDDRGHIRISDLGLAVQIPEGETIRGRVGTVGYMAPEVIQNESYTFSPDWWGLGCLIYEMIQGQSPFRRRKERVKREEVDRRVCEDQEEYTEKFSEDAKDICRQLLCKDPKERLGCRGSGAAEVKLHPIFRNINFKRLEANMLDPPFCPDPRAVYCKDVLDIEQFSTVKGVNLDPTDDDFYHKFVTGSVSIPWQNEMIEMECFKEINVFEMDGALCADLDVNRPNPAPKRGFFYRLFHREASGSASPAVLRGGGA

Target ORF information:

RefSeq Version	XM_009306444.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase 4 (grk4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306444.2

ORF Insert Sequence:

ATGGAGATTG AGAATATCGT GGCCAACACC GTGCTGCTGA AGGCGAGGGA AGGAGGCGGA 
GGGAAGCGTA ATGGACGGAG CAAGAAATGG AAGGAGATGC TGAAATTGCC TCATATCAGT 
CAGTGTGAGG AACTGAGACG CACAATTGAG AGGGATTATA CAAGCCTTTG TGAAAAGCAG 
CCAATTGGAC GTCAGTTGTT TCGTCAATTC TGTGACACTC AGCCCAAATT ACGGCGCTGT 
ATAGAGTTCC TGGATTCTGT GGCAATGTAT CAGGTGGCTC CGGATGAGAA GAGGAGAGAC 
GCCGGTTTAA ATATTTTAGA CACATATTTT AATAACGGGT CAGCCGCTCA TCTACCAGAG 
ATGGCTCAGG ATGTGGTCGG GGAATGCAGG CAGAAACTGG AACAGAGTCC ATGTAAAGAG 
CTGTTCCAGG AGTGTACCAG GATAGTTCGT GAATATTTAA GTGGAGCGCC ATTCTCGGCT 
TATCAGGAGA CTATGTACTT TTCCCGTTTT CTTCAGTGGA AGTGGTTGGA GAGGCAACCA 
GTAACCAAAA ACACATTCAG ACATTACAGG GTATTGGGCA AGGGCGGATT TGGTGAGGTT 
TGTGCCTGCC AGGTTCGTGC CACTGGTAAG ATGTACGCCT GTAAAAAGCT GGAGAAGAAA 
CGTGTGAAGA AAAGAAAAGG TGAAGCAATG GCATTAAATG AAAAACGCAT TTTAGAAAAA 
GTTAACAGTA GTTTTGTAGT TAGTTTGGCA TATGCGTATG AGACTAAGGA CGCTCTATGT 
TTGGTGTTGA CCATAATGAA CGGTGGTGAT CTGAAGTTTC ATATCTATAA CATGGGAAAC 
TCAGGTTTCG ATGAGCAGCG GGCCATCTTT TACGCGGCAG AGATCTGTTG CGGATTAGAG 
GATCTGCATC GAGAGCGGAT CGTCTACAGA GACTTGAAAC CAGAAAATAT TCTGCTAGAT 
GATCGCGGGC ACATCCGTAT TTCTGATCTG GGACTGGCCG TGCAAATTCC AGAGGGCGAG 
ACGATACGAG GGCGTGTTGG CACAGTGGGG TACATGGCAC CAGAGGTCAT CCAGAACGAG 
AGCTACACCT TCAGTCCTGA CTGGTGGGGT TTGGGCTGTC TAATATACGA AATGATTCAG 
GGTCAGTCTC CTTTCCGTCG CCGTAAAGAG CGAGTGAAGA GAGAGGAAGT GGACCGGCGA 
GTTTGTGAGG ACCAGGAGGA ATATACTGAG AAGTTCTCAG AAGACGCTAA AGACATCTGC 
CGTCAGTTGT TATGTAAAGA TCCAAAGGAG CGCTTGGGCT GTCGGGGCAG TGGAGCGGCC 
GAAGTCAAAC TGCATCCCAT TTTCCGCAAC ATCAACTTCA AGCGGCTGGA GGCCAACATG 
CTGGACCCGC CGTTCTGTCC AGACCCGCGA GCAGTTTACT GTAAGGACGT CCTGGACATA 
GAGCAGTTTT CGACAGTCAA AGGTGTAAAC CTGGACCCAA CAGATGACGA TTTCTACCAC 
AAGTTTGTAA CAGGGAGTGT CTCCATCCCA TGGCAAAATG AGATGATTGA GATGGAGTGT 
TTTAAGGAGA TCAACGTGTT CGAGATGGAC GGAGCGCTGT GTGCTGATCT GGATGTGAAC 
AGACCCAATC CAGCACCGAA GCGAGGATTC TTCTACCGTT TATTTCACCG AGAGGCAAGT 
GGTAGTGCAT CGCCCGCCGT GCTCAGAGGG GGAGGGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

An essential role for Grk2 in Hedgehog signalling downstream of Smoothened.
EMBO reports17(5)739-52(2016 05)
Zhao Z,Lee RT,Pusapati GV,Iyu A,Rohatgi R,Ingham PW

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genome-wide RNA interference in Drosophila cells identifies G protein-coupled receptor kinase 2 as a conserved regulator of NF-kappaB signaling.
Journal of immunology (Baltimore, Md. : 1950)184(11)6188-98(2010 Jun)
Valanne S,Myllym?ki H,Kallio J,Schmid MR,Kleino A,Murum?gi A,Airaksinen L,Kotipelto T,Kaustio M,Ulvila J,Esfahani SS,Engstr?m Y,Silvennoinen O,Hultmark D,Parikka M,R?met M

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grk4 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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