tshr cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tshr
Entrez Gene ID	560609
Full Name	thyroid stimulating hormone receptor
General protein information	Preferred Names thyroid stimulating hormone receptor Names thyrotropin receptor TSH receptor manet opallus
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145763.2	NP_001139235.1	thyrotropin receptor precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSHR	NP_000360.2
Mus musculus (house mouse)	Tshr	NP_035778.3
Gallus gallus (chicken)	TSHR	NP_001180517.1
	TSHR	XP_001104839.1
Canis lupus familiaris (dog)	TSHR	NP_001003285.1
Bos taurus (cattle)	TSHR	NP_776631.1
Rattus norvegicus (Norway rat)	Tshr	NP_037020.1
Danio rerio (zebrafish)	tshr	NP_001139235.1
Pan troglodytes (chimpanzee)	TSHR	XP_001166989.1
Xenopus tropicalis (tropical clawed frog)	tshr	XP_002938419.2

Gene Ontology
Bibliography

Related articles in PubMed

Multiple bioanalytical method to reveal developmental biological responses in zebrafish embryos exposed to triclocarban.
Dong X, Xu H, Wu X, Yang L
Chemosphere193251-258(2018 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Functional thyroid differentiation in zebrafish was examined and the role of TSHR signaling during thyroid organogenesis, was characterized.
Title: TSH receptor function is required for normal thyroid differentiation in zebrafish.

The following tshr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tshr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37265	NM_001145763.2 Latest version!	Danio rerio thyroid stimulating hormone receptor (tshr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tshr ( NM_001145763.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139235.1 Danio rerio thyroid stimulating hormone receptor (tshr), mRNA.

Price & Availability↑

CloneID	ODa37265
Clone ID Related Accession (Same CDS sequence)	NM_001145763.2
Accession Version	NM_001145763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	thyrotropin receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF487539.1. On Aug 31, 2012 this sequence version replaced NM_001145763.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF487539.1, CU638786.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505372 [ECO:0000350] ##Evidence-Data-END##

ATGAAGACAC ATGTGATGTT AGTATTGATG CTGCCACTTG TGAGTGTGAT CGGATGGACG 
AATTCATGCC CGCACGGTTG TGAGTGCTCC CAATGGAAAG CCTTAGTTGT CTCATGCATG 
GATGTAGATT CTATTCCAAC GTTACCCGAG AGAACAGAAG TCTTAAGTCT TCACGAGACA 
AGGCTAACAA GAGTTCCCAA AGATGCCTTT GCAAATATGG TCAACATCTC AAGAATCTAT 
CTATCAGTGG ATATCTTTCT GAGCAGGTTA GAAATGCATT CCTTCCACAG CCTGAGAGAG 
CTTACACATA TAGAAATAAG GAACACACGA AAGCTGACAT TCATCCACCC AGAGGCTTTT 
AAGAATCTCC CTAATTTACA GTATCTAGGA TTATTCAACA CAGGCCTCAC CATCTTCCCT 
GACCTGACCA AAATCCAGGC TGAGAATTCA TACTTCATAC TAGAAATTAG TGATAATCCT 
TATATTGCTG AAATTCCAGC CAATGCTTTT TTTGGCATTA CCAACGATCT ACTATCACTG 
AGGCTCTACA GCAACGGCTT TACGAAAGTC CAACCACATG CTTTCAATGG CACAAAGCTG 
GACTCAGTGT ACTTGCATAG AAATAGGGAA CTGACCAGGC TAGATGAGAA AATGTTTGCT 
GGGACAAGTA GTGGGCCAAT GCTCCTTGAT GTGTCCGAAT CAGGAGTTAC TTCTCTCCCA 
GCCAGAGGGT TGGATTCACT CCGGGAACTC TGTGCTCGTA ATGTTTGGGC CCTCAAAAAG 
CTGCCACCAA TCAAGACTTT TAGACACCTA GTTACAGCAG ACTTGACCTA CCCCAGCCAC 
TGCTGTGCGT TCAAGAATTT GAAGAAAAAA CGGGGGTTCA TAGAGGAAAT CATTTGTAAC 
CTGACTGCCC GAAAGAATCA AGTTCAAAAG AGATCTGTGA GAGCTTTCAC AATTCACGGA 
CTGCAGGGCA GCATGGAACC TGGCATCGAC TTGAGTGATA ACCTCTTCAG AGACACCTAT 
GATCACCAGG AGTTTTTCGG CAACGGACAT TATCCCACTT ATTTTGACAG ACATGCAGAT 
GAGAACATGG GTTTCGGTGA AACACTGAAA AACCCCCAGG AGGACACCAG CCGTGAGTTT 
GACAGTCGCT ACGACTACAT TGTGTGCGAG AAGGGAGAGG AGGTTGCTTG TTTCCCTGCT 
CCCGATGAGT TCAACCCTTG CGAGGACATC ATGGGTTTCA GCTTTCTACG AGTGTCTGTG 
TGGTTTGTCA GCTTGCTTGC AGTGTTGGGA AACCTGCTTG TACTTTTGGT GCTTCTGACC 
AGCCACTACA AACTGTCTGT CTCAAGGTTT TTAATGTGCC ACCTGGCGGT AGCTGACCTG 
TGCATGGGCA TTTACCTGCT GCTGATCGCT TCTGTGGACC TCCACACGCA ATCCGAATAT 
TATAATCACG CTATTGATTG GCAGACCGGT CCCGGATGCA GCCTAGCTGG ATTCTTTTCT 
GTCTTCGCTA GTGAACTATC AGTCTACACC CTGACAACTA TCACCCTGGA GCGCTGGTAT 
GCCATCACGT ATGCCATGCG GCTGGACCGT AAGCTGCGTC TCTCCCATGC CTCAGTCATA 
ATGCTAATCG GCTGGATCTT CTGCCTGCTA CTGGGTCTCA TGCCTCTCGT CGGTGTCAGC 
AGCTACCAGA AGGTCAGCAT TTGTCTGCCC ATGGACACTC AGACACTGGT GGACCAGATC 
TACATCATAT GTGTACTCGT TCTCAACATC CTTGCTTTCA TAGTCATTTG TGTCTGTTAC 
ATCAAGATCT ACTGCGCGGT TCACAATCCC AGTCATCAGT CTAGCAACAA AGACACCAAC 
ATTGCCAAGC GTATGGCCGT GCTCATATTC ACTGACTTCC TCTGCATGGC ACCAATTTCA 
TTGTACGCCA TGACTGCCGT TTTAGACCAC CCGCTGATCA CCGTGTCAAA CTCCAAGATC 
CTGCTAGTGC TCTTTTACCC TCTGAATTCC TGCGCCAACC CTTTTCTGTA TGCCATCTTC 
ACCAAGGCCT TCCAAGGGGA TGTCTTTATA CTGCTCAGCA AAGTGGGTCT CTGTCAACGC 
CAGGCTCAGC TCTTTCGAGG ACAGACGGTA TCTTCAAAGG CAAGCAGCAG AGGGATCACC 
AGACACGAGA GGGAGCAAAC AGGAGCAAAC GAGACTCCTA TTTCTCTTCT GGATTTTCCA 
GGCAAAATGG CTGCCGCACA GCAGCAGGAG GACAGTGATG GACAGGGCGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139235.1
CDS	199..2472
Translation	MKTHVMLVLMLPLVSVIGWTNSCPHGCECSQWKALVVSCMDVDSIPTLPERTEVLSLHETRLTRVPKDAFANMVNISRIYLSVDIFLSRLEMHSFHSLRELTHIEIRNTRKLTFIHPEAFKNLPNLQYLGLFNTGLTIFPDLTKIQAENSYFILEISDNPYIAEIPANAFFGITNDLLSLRLYSNGFTKVQPHAFNGTKLDSVYLHRNRELTRLDEKMFAGTSSGPMLLDVSESGVTSLPARGLDSLRELCARNVWALKKLPPIKTFRHLVTADLTYPSHCCAFKNLKKKRGFIEEIICNLTARKNQVQKRSVRAFTIHGLQGSMEPGIDLSDNLFRDTYDHQEFFGNGHYPTYFDRHADENMGFGETLKNPQEDTSREFDSRYDYIVCEKGEEVACFPAPDEFNPCEDIMGFSFLRVSVWFVSLLAVLGNLLVLLVLLTSHYKLSVSRFLMCHLAVADLCMGIYLLLIASVDLHTQSEYYNHAIDWQTGPGCSLAGFFSVFASELSVYTLTTITLERWYAITYAMRLDRKLRLSHASVIMLIGWIFCLLLGLMPLVGVSSYQKVSICLPMDTQTLVDQIYIICVLVLNILAFIVICVCYIKIYCAVHNPSHQSSNKDTNIAKRMAVLIFTDFLCMAPISLYAMTAVLDHPLITVSNSKILLVLFYPLNSCANPFLYAIFTKAFQGDVFILLSKVGLCQRQAQLFRGQTVSSKASSRGITRHEREQTGANETPISLLDFPGKMAAAQQQEDSDGQGA

Target ORF information:

RefSeq Version	NM_001145763.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thyroid stimulating hormone receptor (tshr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145763.2

ORF Insert Sequence:

ATGAAGACAC ATGTGATGTT AGTATTGATG CTGCCACTTG TGAGTGTGAT CGGATGGACG 
AATTCATGCC CGCACGGTTG TGAGTGCTCC CAATGGAAAG CCTTAGTTGT CTCATGCATG 
GATGTAGATT CTATTCCAAC GTTACCCGAG AGAACAGAAG TCTTAAGTCT TCACGAGACA 
AGGCTAACAA GAGTTCCCAA AGATGCCTTT GCAAATATGG TCAACATCTC AAGAATCTAT 
CTATCAGTGG ATATCTTTCT GAGCAGGTTA GAAATGCATT CCTTCCACAG CCTGAGAGAG 
CTTACACATA TAGAAATAAG GAACACACGA AAGCTGACAT TCATCCACCC AGAGGCTTTT 
AAGAATCTCC CTAATTTACA GTATCTAGGA TTATTCAACA CAGGCCTCAC CATCTTCCCT 
GACCTGACCA AAATCCAGGC TGAGAATTCA TACTTCATAC TAGAAATTAG TGATAATCCT 
TATATTGCTG AAATTCCAGC CAATGCTTTT TTTGGCATTA CCAACGATCT ACTATCACTG 
AGGCTCTACA GCAACGGCTT TACGAAAGTC CAACCACATG CTTTCAATGG CACAAAGCTG 
GACTCAGTGT ACTTGCATAG AAATAGGGAA CTGACCAGGC TAGATGAGAA AATGTTTGCT 
GGGACAAGTA GTGGGCCAAT GCTCCTTGAT GTGTCCGAAT CAGGAGTTAC TTCTCTCCCA 
GCCAGAGGGT TGGATTCACT CCGGGAACTC TGTGCTCGTA ATGTTTGGGC CCTCAAAAAG 
CTGCCACCAA TCAAGACTTT TAGACACCTA GTTACAGCAG ACTTGACCTA CCCCAGCCAC 
TGCTGTGCGT TCAAGAATTT GAAGAAAAAA CGGGGGTTCA TAGAGGAAAT CATTTGTAAC 
CTGACTGCCC GAAAGAATCA AGTTCAAAAG AGATCTGTGA GAGCTTTCAC AATTCACGGA 
CTGCAGGGCA GCATGGAACC TGGCATCGAC TTGAGTGATA ACCTCTTCAG AGACACCTAT 
GATCACCAGG AGTTTTTCGG CAACGGACAT TATCCCACTT ATTTTGACAG ACATGCAGAT 
GAGAACATGG GTTTCGGTGA AACACTGAAA AACCCCCAGG AGGACACCAG CCGTGAGTTT 
GACAGTCGCT ACGACTACAT TGTGTGCGAG AAGGGAGAGG AGGTTGCTTG TTTCCCTGCT 
CCCGATGAGT TCAACCCTTG CGAGGACATC ATGGGTTTCA GCTTTCTACG AGTGTCTGTG 
TGGTTTGTCA GCTTGCTTGC AGTGTTGGGA AACCTGCTTG TACTTTTGGT GCTTCTGACC 
AGCCACTACA AACTGTCTGT CTCAAGGTTT TTAATGTGCC ACCTGGCGGT AGCTGACCTG 
TGCATGGGCA TTTACCTGCT GCTGATCGCT TCTGTGGACC TCCACACGCA ATCCGAATAT 
TATAATCACG CTATTGATTG GCAGACCGGT CCCGGATGCA GCCTAGCTGG ATTCTTTTCT 
GTCTTCGCTA GTGAACTATC AGTCTACACC CTGACAACTA TCACCCTGGA GCGCTGGTAT 
GCCATCACGT ATGCCATGCG GCTGGACCGT AAGCTGCGTC TCTCCCATGC CTCAGTCATA 
ATGCTAATCG GCTGGATCTT CTGCCTGCTA CTGGGTCTCA TGCCTCTCGT CGGTGTCAGC 
AGCTACCAGA AGGTCAGCAT TTGTCTGCCC ATGGACACTC AGACACTGGT GGACCAGATC 
TACATCATAT GTGTACTCGT TCTCAACATC CTTGCTTTCA TAGTCATTTG TGTCTGTTAC 
ATCAAGATCT ACTGCGCGGT TCACAATCCC AGTCATCAGT CTAGCAACAA AGACACCAAC 
ATTGCCAAGC GTATGGCCGT GCTCATATTC ACTGACTTCC TCTGCATGGC ACCAATTTCA 
TTGTACGCCA TGACTGCCGT TTTAGACCAC CCGCTGATCA CCGTGTCAAA CTCCAAGATC 
CTGCTAGTGC TCTTTTACCC TCTGAATTCC TGCGCCAACC CTTTTCTGTA TGCCATCTTC 
ACCAAGGCCT TCCAAGGGGA TGTCTTTATA CTGCTCAGCA AAGTGGGTCT CTGTCAACGC 
CAGGCTCAGC TCTTTCGAGG ACAGACGGTA TCTTCAAAGG CAAGCAGCAG AGGGATCACC 
AGACACGAGA GGGAGCAAAC AGGAGCAAAC GAGACTCCTA TTTCTCTTCT GGATTTTCCA 
GGCAAAATGG CTGCCGCACA GCAGCAGGAG GACAGTGATG GACAGGGCGC ATGA