casr cDNA ORF clone, Danio rerio(zebrafish)

The following casr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the casr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa11241 NM_001351665.1
Latest version!
Danio rerio calcium-sensing receptor (casr), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa11241 XM_684005.5
Latest version!
Danio rerio calcium sensing receptor (casr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa59325 XM_017356412.2
Latest version!
Danio rerio calcium-sensing receptor (casr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
ODa59325 XM_017356412.1 Danio rerio calcium sensing receptor (casr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa11241
    Clone ID Related Accession (Same CDS sequence) XM_684005.5 , NM_001351665.1
    Accession Version NM_001351665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-30
    Organism Danio rerio(zebrafish)
    Product extracellular calcium-sensing receptor precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LO017805.1 and FQ790224.5. On May 18, 2017 this sequence version replaced XM_684005.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01023288.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA4476745, SAMEA4476748 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTACC ATTTTCCAAC TGTAGTGTGG AATGTGAACC TGGAACAAGG
    AAAGGAATTA TTGATGGCGA ACCCACATGT TGCTTTGAGT GTACAGAATG CTCGGATGGG
    GAGTACAGTG ATCATAAAGA TGCCAGTTTT TGCGTTAAAT GCCCAAACAA CTCCTGGTCC
    AACGGCAATC ACACTTCTTG CTTTCTGAAG CAAATCGAGT TTCTGTCCTG GACCGAACCG
    TTCGGGATTG CGCTGGCCTT ATTTGCAGTC CTCGGGGTTC TCCTAACAGC TTTTGTGTTG
    GGTGTTTTTG TGCAATTCCG TGATACTCCA ATCGTGAAGG CATCAAACCG AGAGCTGTCG
    TTTCTTTTGC TTTTCTCGCT TATATGCTGT TTCTCCAGCT CTCTCATATT CATAGGCGAA
    CCACAGGATT GGACGTGCCG GGTACGCCAA CCAGCATTCG GCATCAGCTT TGTGTTATGC
    ATCTCATGCA TTCTAGTCAA AACCAATCGT GTCCTGCTGG TGTTCGAAGC TAAAATCCCT
    ACGAGTCTCC ACCGTAAGTG GTGGGGATTA AACCTGCAGT TCTTGCTAGT CTTCTTGTTC
    ACGTTTGTGC AGGTGATGAT CTGCGTGGTT TGGTTGTACA ACGCTCCGCC TGGAAGTTAC
    AAGAACTACG ACATCGATGA AATCATCTTC ATCACCTGCA ACGAGGGCTC CATGATGGCT
    CTGGGGTTTC TGATTGGCTA CACGTGCTTG TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC
    AAGTCTCGGA AGCTGCCGGA GAATTTCACG GAGGCCAAAT TCATAACGTT CAGCATGCTG
    ATTTTTTTCA TCGTCTGGAT CTCCTTTATT CCGGCGTACT TCAGCACTTA CGGCAAATTC
    GTCTCGGCTG TTGAGGTCAT AGCAATCCTG GCGTCCAGCT TCAGTTTGCT TGCCTGTATC
    TTTTTCAACA AGGTGTATAT CATTCTTCTG AAACCGTCGA GGAATACGAT CGAGGAGGTG
    CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA GCTGCAGCAA AAGCGACTTT ACGACACAGT
    TCAGGCTTTA GAAAGAGATC CAGCAGTGTT GGCGGCTCCT CCGCATCTTC GCCCTCCTCA
    TCCATCAGCA TGAAGACCAA CGGGAATGAA ATGGAGTCAC CGTCTACGCG GAGACACGGC
    TCAAAACCGA GGGTGAGCTT TGGAAGTGGG ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG
    GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001338594.1
    CDS559..3399
    Translation

    Target ORF information:

    RefSeq Version NM_001351665.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium-sensing receptor (casr), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001351665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTACC ATTTTCCAAC TGTAGTGTGG AATGTGAACC TGGAACAAGG
    AAAGGAATTA TTGATGGCGA ACCCACATGT TGCTTTGAGT GTACAGAATG CTCGGATGGG
    GAGTACAGTG ATCATAAAGA TGCCAGTTTT TGCGTTAAAT GCCCAAACAA CTCCTGGTCC
    AACGGCAATC ACACTTCTTG CTTTCTGAAG CAAATCGAGT TTCTGTCCTG GACCGAACCG
    TTCGGGATTG CGCTGGCCTT ATTTGCAGTC CTCGGGGTTC TCCTAACAGC TTTTGTGTTG
    GGTGTTTTTG TGCAATTCCG TGATACTCCA ATCGTGAAGG CATCAAACCG AGAGCTGTCG
    TTTCTTTTGC TTTTCTCGCT TATATGCTGT TTCTCCAGCT CTCTCATATT CATAGGCGAA
    CCACAGGATT GGACGTGCCG GGTACGCCAA CCAGCATTCG GCATCAGCTT TGTGTTATGC
    ATCTCATGCA TTCTAGTCAA AACCAATCGT GTCCTGCTGG TGTTCGAAGC TAAAATCCCT
    ACGAGTCTCC ACCGTAAGTG GTGGGGATTA AACCTGCAGT TCTTGCTAGT CTTCTTGTTC
    ACGTTTGTGC AGGTGATGAT CTGCGTGGTT TGGTTGTACA ACGCTCCGCC TGGAAGTTAC
    AAGAACTACG ACATCGATGA AATCATCTTC ATCACCTGCA ACGAGGGCTC CATGATGGCT
    CTGGGGTTTC TGATTGGCTA CACGTGCTTG TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC
    AAGTCTCGGA AGCTGCCGGA GAATTTCACG GAGGCCAAAT TCATAACGTT CAGCATGCTG
    ATTTTTTTCA TCGTCTGGAT CTCCTTTATT CCGGCGTACT TCAGCACTTA CGGCAAATTC
    GTCTCGGCTG TTGAGGTCAT AGCAATCCTG GCGTCCAGCT TCAGTTTGCT TGCCTGTATC
    TTTTTCAACA AGGTGTATAT CATTCTTCTG AAACCGTCGA GGAATACGAT CGAGGAGGTG
    CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA GCTGCAGCAA AAGCGACTTT ACGACACAGT
    TCAGGCTTTA GAAAGAGATC CAGCAGTGTT GGCGGCTCCT CCGCATCTTC GCCCTCCTCA
    TCCATCAGCA TGAAGACCAA CGGGAATGAA ATGGAGTCAC CGTCTACGCG GAGACACGGC
    TCAAAACCGA GGGTGAGCTT TGGAAGTGGG ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG
    GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa11241
    Clone ID Related Accession (Same CDS sequence) XM_684005.5 , NM_001351665.1
    Accession Version XM_684005.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product extracellular calcium-sensing receptor isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_684005.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTACC ATTTTCCAAC TGTAGTGTGG AATGTGAACC TGGAACAAGG
    AAAGGAATTA TTGATGGCGA ACCCACATGT TGCTTTGAGT GTACAGAATG CTCGGATGGG
    GAGTACAGTG ATCATAAAGA TGCCAGTTTT TGCGTTAAAT GCCCAAACAA CTCCTGGTCC
    AACGGCAATC ACACTTCTTG CTTTCTGAAG CAAATCGAGT TTCTGTCCTG GACCGAACCG
    TTCGGGATTG CGCTGGCCTT ATTTGCAGTC CTCGGGGTTC TCCTAACAGC TTTTGTGTTG
    GGTGTTTTTG TGCAATTCCG TGATACTCCA ATCGTGAAGG CATCAAACCG AGAGCTGTCG
    TTTCTTTTGC TTTTCTCGCT TATATGCTGT TTCTCCAGCT CTCTCATATT CATAGGCGAA
    CCACAGGATT GGACGTGCCG GGTACGCCAA CCAGCATTCG GCATCAGCTT TGTGTTATGC
    ATCTCATGCA TTCTAGTCAA AACCAATCGT GTCCTGCTGG TGTTCGAAGC TAAAATCCCT
    ACGAGTCTCC ACCGTAAGTG GTGGGGATTA AACCTGCAGT TCTTGCTAGT CTTCTTGTTC
    ACGTTTGTGC AGGTGATGAT CTGCGTGGTT TGGTTGTACA ACGCTCCGCC TGGAAGTTAC
    AAGAACTACG ACATCGATGA AATCATCTTC ATCACCTGCA ACGAGGGCTC CATGATGGCT
    CTGGGGTTTC TGATTGGCTA CACGTGCTTG TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC
    AAGTCTCGGA AGCTGCCGGA GAATTTCACG GAGGCCAAAT TCATAACGTT CAGCATGCTG
    ATTTTTTTCA TCGTCTGGAT CTCCTTTATT CCGGCGTACT TCAGCACTTA CGGCAAATTC
    GTCTCGGCTG TTGAGGTCAT AGCAATCCTG GCGTCCAGCT TCAGTTTGCT TGCCTGTATC
    TTTTTCAACA AGGTGTATAT CATTCTTCTG AAACCGTCGA GGAATACGAT CGAGGAGGTG
    CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA GCTGCAGCAA AAGCGACTTT ACGACACAGT
    TCAGGCTTTA GAAAGAGATC CAGCAGTGTT GGCGGCTCCT CCGCATCTTC GCCCTCCTCA
    TCCATCAGCA TGAAGACCAA CGGGAATGAA ATGGAGTCAC CGTCTACGCG GAGACACGGC
    TCAAAACCGA GGGTGAGCTT TGGAAGTGGG ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG
    GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_689097.1
    CDS389..3229
    Translation

    Target ORF information:

    RefSeq Version XM_684005.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium sensing receptor (casr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_684005.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTACC ATTTTCCAAC TGTAGTGTGG AATGTGAACC TGGAACAAGG
    AAAGGAATTA TTGATGGCGA ACCCACATGT TGCTTTGAGT GTACAGAATG CTCGGATGGG
    GAGTACAGTG ATCATAAAGA TGCCAGTTTT TGCGTTAAAT GCCCAAACAA CTCCTGGTCC
    AACGGCAATC ACACTTCTTG CTTTCTGAAG CAAATCGAGT TTCTGTCCTG GACCGAACCG
    TTCGGGATTG CGCTGGCCTT ATTTGCAGTC CTCGGGGTTC TCCTAACAGC TTTTGTGTTG
    GGTGTTTTTG TGCAATTCCG TGATACTCCA ATCGTGAAGG CATCAAACCG AGAGCTGTCG
    TTTCTTTTGC TTTTCTCGCT TATATGCTGT TTCTCCAGCT CTCTCATATT CATAGGCGAA
    CCACAGGATT GGACGTGCCG GGTACGCCAA CCAGCATTCG GCATCAGCTT TGTGTTATGC
    ATCTCATGCA TTCTAGTCAA AACCAATCGT GTCCTGCTGG TGTTCGAAGC TAAAATCCCT
    ACGAGTCTCC ACCGTAAGTG GTGGGGATTA AACCTGCAGT TCTTGCTAGT CTTCTTGTTC
    ACGTTTGTGC AGGTGATGAT CTGCGTGGTT TGGTTGTACA ACGCTCCGCC TGGAAGTTAC
    AAGAACTACG ACATCGATGA AATCATCTTC ATCACCTGCA ACGAGGGCTC CATGATGGCT
    CTGGGGTTTC TGATTGGCTA CACGTGCTTG TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC
    AAGTCTCGGA AGCTGCCGGA GAATTTCACG GAGGCCAAAT TCATAACGTT CAGCATGCTG
    ATTTTTTTCA TCGTCTGGAT CTCCTTTATT CCGGCGTACT TCAGCACTTA CGGCAAATTC
    GTCTCGGCTG TTGAGGTCAT AGCAATCCTG GCGTCCAGCT TCAGTTTGCT TGCCTGTATC
    TTTTTCAACA AGGTGTATAT CATTCTTCTG AAACCGTCGA GGAATACGAT CGAGGAGGTG
    CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA GCTGCAGCAA AAGCGACTTT ACGACACAGT
    TCAGGCTTTA GAAAGAGATC CAGCAGTGTT GGCGGCTCCT CCGCATCTTC GCCCTCCTCA
    TCCATCAGCA TGAAGACCAA CGGGAATGAA ATGGAGTCAC CGTCTACGCG GAGACACGGC
    TCAAAACCGA GGGTGAGCTT TGGAAGTGGG ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG
    GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59325
    Clone ID Related Accession (Same CDS sequence) XM_017356412.2 , XM_017356412.1
    Accession Version XM_017356412.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTAAT GTGTTGTTTC TCTTTTCTCT ACCAGGTACC ATTTTCCAAC
    TGTAGTGTGG AATGTGAACC TGGAACAAGG AAAGGAATTA TTGATGGCGA ACCCACATGT
    TGCTTTGAGT GTACAGAATG CTCGGATGGG GAGTACAGTG ATCATAAAGA TGCCAGTTTT
    TGCGTTAAAT GCCCAAACAA CTCCTGGTCC AACGGCAATC ACACTTCTTG CTTTCTGAAG
    CAAATCGAGT TTCTGTCCTG GACCGAACCG TTCGGGATTG CGCTGGCCTT ATTTGCAGTC
    CTCGGGGTTC TCCTAACAGC TTTTGTGTTG GGTGTTTTTG TGCAATTCCG TGATACTCCA
    ATCGTGAAGG CATCAAACCG AGAGCTGTCG TTTCTTTTGC TTTTCTCGCT TATATGCTGT
    TTCTCCAGCT CTCTCATATT CATAGGCGAA CCACAGGATT GGACGTGCCG GGTACGCCAA
    CCAGCATTCG GCATCAGCTT TGTGTTATGC ATCTCATGCA TTCTAGTCAA AACCAATCGT
    GTCCTGCTGG TGTTCGAAGC TAAAATCCCT ACGAGTCTCC ACCGTAAGTG GTGGGGATTA
    AACCTGCAGT TCTTGCTAGT CTTCTTGTTC ACGTTTGTGC AGGTGATGAT CTGCGTGGTT
    TGGTTGTACA ACGCTCCGCC TGGAAGTTAC AAGAACTACG ACATCGATGA AATCATCTTC
    ATCACCTGCA ACGAGGGCTC CATGATGGCT CTGGGGTTTC TGATTGGCTA CACGTGCTTG
    TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC AAGTCTCGGA AGCTGCCGGA GAATTTCACG
    GAGGCCAAAT TCATAACGTT CAGCATGCTG ATTTTTTTCA TCGTCTGGAT CTCCTTTATT
    CCGGCGTACT TCAGCACTTA CGGCAAATTC GTCTCGGCTG TTGAGGTCAT AGCAATCCTG
    GCGTCCAGCT TCAGTTTGCT TGCCTGTATC TTTTTCAACA AGGTGTATAT CATTCTTCTG
    AAACCGTCGA GGAATACGAT CGAGGAGGTG CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA
    GCTGCAGCAA AAGCGACTTT ACGACACAGT TCAGGCTTTA GAAAGAGATC CAGCAGTGTT
    GGCGGCTCCT CCGCATCTTC GCCCTCCTCA TCCATCAGCA TGAAGACCAA CGGGAATGAA
    ATGGAGTCAC CGTCTACGCG GAGACACGGC TCAAAACCGA GGGTGAGCTT TGGAAGTGGG
    ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211901.1
    CDS474..3344
    Translation

    Target ORF information:

    RefSeq Version XM_017356412.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium-sensing receptor (casr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTAAT GTGTTGTTTC TCTTTTCTCT ACCAGGTACC ATTTTCCAAC
    TGTAGTGTGG AATGTGAACC TGGAACAAGG AAAGGAATTA TTGATGGCGA ACCCACATGT
    TGCTTTGAGT GTACAGAATG CTCGGATGGG GAGTACAGTG ATCATAAAGA TGCCAGTTTT
    TGCGTTAAAT GCCCAAACAA CTCCTGGTCC AACGGCAATC ACACTTCTTG CTTTCTGAAG
    CAAATCGAGT TTCTGTCCTG GACCGAACCG TTCGGGATTG CGCTGGCCTT ATTTGCAGTC
    CTCGGGGTTC TCCTAACAGC TTTTGTGTTG GGTGTTTTTG TGCAATTCCG TGATACTCCA
    ATCGTGAAGG CATCAAACCG AGAGCTGTCG TTTCTTTTGC TTTTCTCGCT TATATGCTGT
    TTCTCCAGCT CTCTCATATT CATAGGCGAA CCACAGGATT GGACGTGCCG GGTACGCCAA
    CCAGCATTCG GCATCAGCTT TGTGTTATGC ATCTCATGCA TTCTAGTCAA AACCAATCGT
    GTCCTGCTGG TGTTCGAAGC TAAAATCCCT ACGAGTCTCC ACCGTAAGTG GTGGGGATTA
    AACCTGCAGT TCTTGCTAGT CTTCTTGTTC ACGTTTGTGC AGGTGATGAT CTGCGTGGTT
    TGGTTGTACA ACGCTCCGCC TGGAAGTTAC AAGAACTACG ACATCGATGA AATCATCTTC
    ATCACCTGCA ACGAGGGCTC CATGATGGCT CTGGGGTTTC TGATTGGCTA CACGTGCTTG
    TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC AAGTCTCGGA AGCTGCCGGA GAATTTCACG
    GAGGCCAAAT TCATAACGTT CAGCATGCTG ATTTTTTTCA TCGTCTGGAT CTCCTTTATT
    CCGGCGTACT TCAGCACTTA CGGCAAATTC GTCTCGGCTG TTGAGGTCAT AGCAATCCTG
    GCGTCCAGCT TCAGTTTGCT TGCCTGTATC TTTTTCAACA AGGTGTATAT CATTCTTCTG
    AAACCGTCGA GGAATACGAT CGAGGAGGTG CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA
    GCTGCAGCAA AAGCGACTTT ACGACACAGT TCAGGCTTTA GAAAGAGATC CAGCAGTGTT
    GGCGGCTCCT CCGCATCTTC GCCCTCCTCA TCCATCAGCA TGAAGACCAA CGGGAATGAA
    ATGGAGTCAC CGTCTACGCG GAGACACGGC TCAAAACCGA GGGTGAGCTT TGGAAGTGGG
    ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59325
    Clone ID Related Accession (Same CDS sequence) XM_017356412.2 , XM_017356412.1
    Accession Version XM_017356412.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product extracellular calcium-sensing receptor isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTAAT GTGTTGTTTC TCTTTTCTCT ACCAGGTACC ATTTTCCAAC
    TGTAGTGTGG AATGTGAACC TGGAACAAGG AAAGGAATTA TTGATGGCGA ACCCACATGT
    TGCTTTGAGT GTACAGAATG CTCGGATGGG GAGTACAGTG ATCATAAAGA TGCCAGTTTT
    TGCGTTAAAT GCCCAAACAA CTCCTGGTCC AACGGCAATC ACACTTCTTG CTTTCTGAAG
    CAAATCGAGT TTCTGTCCTG GACCGAACCG TTCGGGATTG CGCTGGCCTT ATTTGCAGTC
    CTCGGGGTTC TCCTAACAGC TTTTGTGTTG GGTGTTTTTG TGCAATTCCG TGATACTCCA
    ATCGTGAAGG CATCAAACCG AGAGCTGTCG TTTCTTTTGC TTTTCTCGCT TATATGCTGT
    TTCTCCAGCT CTCTCATATT CATAGGCGAA CCACAGGATT GGACGTGCCG GGTACGCCAA
    CCAGCATTCG GCATCAGCTT TGTGTTATGC ATCTCATGCA TTCTAGTCAA AACCAATCGT
    GTCCTGCTGG TGTTCGAAGC TAAAATCCCT ACGAGTCTCC ACCGTAAGTG GTGGGGATTA
    AACCTGCAGT TCTTGCTAGT CTTCTTGTTC ACGTTTGTGC AGGTGATGAT CTGCGTGGTT
    TGGTTGTACA ACGCTCCGCC TGGAAGTTAC AAGAACTACG ACATCGATGA AATCATCTTC
    ATCACCTGCA ACGAGGGCTC CATGATGGCT CTGGGGTTTC TGATTGGCTA CACGTGCTTG
    TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC AAGTCTCGGA AGCTGCCGGA GAATTTCACG
    GAGGCCAAAT TCATAACGTT CAGCATGCTG ATTTTTTTCA TCGTCTGGAT CTCCTTTATT
    CCGGCGTACT TCAGCACTTA CGGCAAATTC GTCTCGGCTG TTGAGGTCAT AGCAATCCTG
    GCGTCCAGCT TCAGTTTGCT TGCCTGTATC TTTTTCAACA AGGTGTATAT CATTCTTCTG
    AAACCGTCGA GGAATACGAT CGAGGAGGTG CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA
    GCTGCAGCAA AAGCGACTTT ACGACACAGT TCAGGCTTTA GAAAGAGATC CAGCAGTGTT
    GGCGGCTCCT CCGCATCTTC GCCCTCCTCA TCCATCAGCA TGAAGACCAA CGGGAATGAA
    ATGGAGTCAC CGTCTACGCG GAGACACGGC TCAAAACCGA GGGTGAGCTT TGGAAGTGGG
    ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211901.1
    CDS389..3259
    Translation

    Target ORF information:

    RefSeq Version XM_017356412.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium sensing receptor (casr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGGTTTC ATCTGAAGTT TTACCTGCAT TACCTGGTTC TACTGGGTTC CAGCTGTGTA 
    ATTTCGACCT ATGGCCCAAA CCAGAGGGCC CAGAAGACGG GCGATATCCT GCTTGGAGGA
    CTTTTCCCAA TGCATTTTGG GGTGGCCTCC AAAGACCAGG ATCTTGCAGC ACGGCCAGAG
    TCAACAGAGT GTGTCAGATA TAACTTCCGT GGATTCCGCT GGCTGCAGTC TATGATCTTC
    GCTATAGAGG AGATCAATAA CAGCTCCACT CTTCTTCCAA ACATTACGCT TGGCTACCGT
    ATATTTGACA CGTGCAACAC TGTCTCGAAA GCGTTGGAGG CGTCACTGAG TTTTGTCGCG
    CAGAATAAGA TTGATTCACT GAATCTGGAC GAGTTCTGTA ACTGCACAGG GAACATCCCA
    TCAACCATTG CAGTGGTCGG GGCTTCTGGA TCTGCAGTGT CTACTGCCGT GGCGGACCTG
    CTGGGTCTTT TCTACATCCC TCAGATTAGC TATGCTTCAT CCAGCCGCCT TTTGAGCAAC
    AAAAACCAGT ACAAATCCTT CATGAGGACA ATCCCTACAG ACGAGTACCA GGCGATTGCC
    ATGGCTGCCA TCATCGAGCA CTTCCAGTGG AACTGGGTGA TTGCTATCGC CTCTGACGAC
    GAATACGGCC GGCCAGGCAT TGAAAAGTTT GAGAATGAAA TGTTTCACCG GGACATCTGC
    ATTGACCTCA ATGTTTTAAT TTCACAGTAC GTTGACGAAG CTGAGATTCG TCGCCTGGCA
    GATCGCATCC AGAACTCATC CGCCAAGGTA ATTGTTGTGT TTGCTAGCGG ACCAGATATC
    GAGCCGTTAG TTAAAGAGAT GGTGAGACGA AACATCACAG ACCGCGTCTG GTTGGCGAGC
    GAAGCATGGG CCAGTTCCAG TCTAGTCGCC AAACCAGAGT ATCTTGACGT CATGGGAGGA
    ACCATTGGCT TTGCTCTGAG GGCTGGACAT ATACCCGGCT TTAAAGACTT CTTACAGCAG
    GTCCATCCAA AGAAGTCAAG CCATAATGAA TTTGTGCGAG AATTTTGGGA GGAGACGTTC
    AACTGTTATC TTGAAGACAG CCCAAGGAAT GCTGATAGTG AGAATGGCAG TACGAGTTTC
    AGGCCGCTGT GTACTGGGGA AGAGGACATT GCCAGTGTTG AGACGCCATA TTTGGACTAC
    ACGCACCTCA GGATTTCCTA TAACGTGTAT GTGGCTGTTT ATGCCATAGC ACAGGCACTT
    CAGGACATAC TTACCTGCAC TCCAGGGAAA GGATTGTTTT CTAATGGCTC TTGTGCAGAT
    ATTAGGAAAG TTGAAGCTTG GCAGGTTCTG AAACAACTAA GACACCTCAA CTTCATAGAT
    AGCATGGGGG AGAGAGTGCG CTTTGACAAC GGCAGTGAGC TTTCAGCCAA TTATACCATC
    ATAAACTGGC ATCGATCACC CGAGGATGGG TCTGTCGTAT TCAAGGAGGT TGGCTATTAC
    AGTATACATA ACAAGAACGT GGCCAAGCTT TCCATTGACA AGAGCAAAAT CCTGTGGAAC
    GGACGTCTAA CTGAGGTAAT GTGTTGTTTC TCTTTTCTCT ACCAGGTACC ATTTTCCAAC
    TGTAGTGTGG AATGTGAACC TGGAACAAGG AAAGGAATTA TTGATGGCGA ACCCACATGT
    TGCTTTGAGT GTACAGAATG CTCGGATGGG GAGTACAGTG ATCATAAAGA TGCCAGTTTT
    TGCGTTAAAT GCCCAAACAA CTCCTGGTCC AACGGCAATC ACACTTCTTG CTTTCTGAAG
    CAAATCGAGT TTCTGTCCTG GACCGAACCG TTCGGGATTG CGCTGGCCTT ATTTGCAGTC
    CTCGGGGTTC TCCTAACAGC TTTTGTGTTG GGTGTTTTTG TGCAATTCCG TGATACTCCA
    ATCGTGAAGG CATCAAACCG AGAGCTGTCG TTTCTTTTGC TTTTCTCGCT TATATGCTGT
    TTCTCCAGCT CTCTCATATT CATAGGCGAA CCACAGGATT GGACGTGCCG GGTACGCCAA
    CCAGCATTCG GCATCAGCTT TGTGTTATGC ATCTCATGCA TTCTAGTCAA AACCAATCGT
    GTCCTGCTGG TGTTCGAAGC TAAAATCCCT ACGAGTCTCC ACCGTAAGTG GTGGGGATTA
    AACCTGCAGT TCTTGCTAGT CTTCTTGTTC ACGTTTGTGC AGGTGATGAT CTGCGTGGTT
    TGGTTGTACA ACGCTCCGCC TGGAAGTTAC AAGAACTACG ACATCGATGA AATCATCTTC
    ATCACCTGCA ACGAGGGCTC CATGATGGCT CTGGGGTTTC TGATTGGCTA CACGTGCTTG
    TTGGCCGCCA TCTGTTTTTT CTTCGCGTTC AAGTCTCGGA AGCTGCCGGA GAATTTCACG
    GAGGCCAAAT TCATAACGTT CAGCATGCTG ATTTTTTTCA TCGTCTGGAT CTCCTTTATT
    CCGGCGTACT TCAGCACTTA CGGCAAATTC GTCTCGGCTG TTGAGGTCAT AGCAATCCTG
    GCGTCCAGCT TCAGTTTGCT TGCCTGTATC TTTTTCAACA AGGTGTATAT CATTCTTCTG
    AAACCGTCGA GGAATACGAT CGAGGAGGTG CGCTGTAGCA CTGCGGCTCA CGCCTTTAAA
    GCTGCAGCAA AAGCGACTTT ACGACACAGT TCAGGCTTTA GAAAGAGATC CAGCAGTGTT
    GGCGGCTCCT CCGCATCTTC GCCCTCCTCA TCCATCAGCA TGAAGACCAA CGGGAATGAA
    ATGGAGTCAC CGTCTACGCG GAGACACGGC TCAAAACCGA GGGTGAGCTT TGGAAGTGGG
    ACGGTCAGTC TCTCCTTGAG CTTCGAGGAG GCTAGAAACT CGATGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.