dcaf5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dcaf5
Entrez Gene ID	560257
Full Name	ddb1 and cul4 associated factor 5
Synonyms	wdr22,zgc:153934
General protein information	Preferred Names ddb1 and cul4 associated factor 5 Names DDB1- and CUL4-associated factor 5 WD repeat domain 22
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077552.1	NP_001071020.1	DDB1- and CUL4-associated factor 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	DCAF5	NP_001271135.1
	DCAF5	XP_001104318.2
Canis lupus familiaris (dog)	DCAF5	XP_547871.3
Bos taurus (cattle)	DCAF5	XP_002691087.2
Danio rerio (zebrafish)	dcaf5	NP_001071020.1
Xenopus tropicalis (tropical clawed frog)	dcaf5	XP_002935378.2
Pan troglodytes (chimpanzee)	DCAF5	XP_510028.3
Mus musculus (house mouse)	Dcaf5	NP_796241.3
Gallus gallus (chicken)	DCAF5	XP_426432.4
Rattus norvegicus (Norway rat)	Dcaf5	NP_001094188.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates.
Holterhoff CK, Saunders RH, Brito EE, Wagner DS
Developmental dynamics : an official publication of the American Association of Anatomists238(11)2936-47(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dcaf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dcaf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37256	NM_001077552.1 Latest version!	Danio rerio ddb1 and cul4 associated factor 5 (dcaf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dcaf5 ( NM_001077552.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001071020.1 Danio rerio ddb1 and cul4 associated factor 5 (dcaf5), mRNA.

Price & Availability↑

CloneID	ODa37256
Clone ID Related Accession (Same CDS sequence)	NM_001077552.1
Accession Version	NM_001077552.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DDB1- and CUL4-associated factor 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC124494.1. On Nov 9, 2006 this sequence version replaced XM_683652.1. ##Evidence-Data-START## Transcript exon combination :: BC124494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAAGGAGG CGCGGGGCTG CAGCATGCGG AGCGCCGTCG GGTTTCTCTC CCGCAGGACG 
ATCGACGGAC ACCCGCAGCT GAAGGAGGAG TTCCAGCGCC GCAGACTGTC CGGCTGCTCT 
AGCCTCTACA AGAAGGACAT GCTCGGACAC TTCGGCTGCG TCAACGCCAT CGAGTTCTCC 
AACAATGGAG GCCAGTGGCT GGTGTCCGGG GGAGACGACC GCAGGGTGTT GCTGTGGCAC 
ATGGAGAAAG CAATACACTC ACGGGCAAAA CCAATGAAGC TGAAGGGGGA GCACCTGTCC 
AACATCTTCT GCCTGGCGTT TGACAGCACC AACAAGCGTG TTTTCTCAGG AGGAAATGAT 
GAGCAGGTGA TTCTGCATGA CGTGGAACGT GGGGAGACGC TCAATGTCTT CTTGCATGAT 
GACGCCGTGT ATGGTCTGTC CGTCAGTCCG GTTAATGATA ACGTCTTCGC CAGCTCCTCT 
GATGACGGAA GAGTTCTCAT ATGGGATACC AGAGAGCCAC CACATGGAGA ACCGTTCTGC 
TTGGCCAACT ACCCCTCAGC ATTTCACAGT GTGATGTTTA ACCCTGTTGA GCCTCGTCTG 
CTGGCCACTG CAAACTCTAA AGAGGGAGTT GGACTATGGG ATATCCGCAA ACCCCGCAGT 
TCTCTGTTGC GGTACGGAGG CAGTTTGTCT CTTCAGAGTG CCATGAGTGT TCGCTTTAAC 
AGCACGGGGA CGCAGCTGCT GGCTCTGCGC AGACGTCTGC CACCCGTCCT CTATGAGCTC 
CACTCTCGTC TGCCCAGCTT CCAGTTTGAT AACCAGGGCT ACTTCAACTC CTGCACTATG 
AAGAGCTGCT GTTTCGCTGG CGACCGAGAT CAGTACATCT TGTCTGGCTC TGATGATTTT 
AACCTTTACA TGTGGAGAAT TCCCACTGAC CCTGAAGCAG GAGGCGCGGG TCGAGTGGTG 
AATGGGGCCT TTATGGTTTT AAAAGGTCAT CGGTCCATTG TGAATCAGGT CCGCTTTAAC 
CCTCACACGT ACATGATCTG CTCCTCCGGG GTGGAGAAGG TCATCAAGGT TTGGAGTCCG 
TATAAGCAGC CAGACAGTTT AGGAGATCTA GAAGGCCTGG TGGAAGACAA GTCTCGCTCT 
CTCTACACTC ATGAGGAATA CATAAGCCTG GTACTCAACA GCGGGACCGG CCTGTCACAC 
GATTATGTCA GCCAGTCAGT GCAGGAAGAC CCTCGAATGA TGGCCTTTTT TGACTCACTG 
GTTCGGCGTG AGATCGAGGG CTGGAGCTCG GACTCTGACA GCGATTTGAG CGAGGGTGCG 
ATTCTGCAGC TGCATGCCAG AGCCCGGCGG TCGACCCGCT CCACACGATC CACACCACGA 
TCTCCTGCTG CTGTGTCTGC CGCTCATCAT AGCGACTCCG AACACTCGTC TTCATCTTCT 
CTAGCGGGAC CTGATGTGTC CGGGAGCGAA CGGACGGATC AGGATAGAAG TGGGTCACAG 
AGGAACCGGG CCAGACCACG TGCGTCTGCG TTTCTGCTTG ATCTTGTGAA CGAGGATTCA 
GATTCCAGTG GGTTTTGGTT AGACCCGATG CCCCGTCCCA GATCCCCTAG CCCACGTGAA 
AACTCAAGTC TTTCTAGCCA CGCCTCCTCA GCGGGCGTAG CCTCCAGTTC AAGCTCCTCC 
CCCAGCTCAT CTTCTAGCTC CTCCTCCAGC ACTGATGGAG TTGACGAGGA GCTGCGCAGG 
AGCAGGATGC GACAGCGCAA TGCAGCCAGA TGGCGCCGCC ATATTGGCTT TTCTCCAAGA 
GACTCGGCCC AAACACTTCA CAGCGCCACA GACTCCTCCA ACTATCCCAA CATTTCCATC 
CACGATCAGT CGTCCCCCTC TTCGTCCTCA GGTGACGAAG ATCGGCCTTT CAGAAATCGT 
GGAAAACAAA AGCCAAGTCC GTCGCACTTA TCTGACAGGC AATCGCAGTC CAGATGTAGG 
CGCCGCATCA CTTCTTCCTT CTCAGGGGCT GGGGACGGCC CTTTGGAGAG GGCGAAATCC 
CGAGCAGGCT TTCTGGCGGA GTCTGGGGAG GAACTGGAGC GAGGGAGAAC TAGCGGAGAA 
GAGTTGAGTA CTGTGGAGGA TTTAAACCTG ACACTCAGCA GTCGGACCAC AGTGGCTGGT 
GTTAACGGAC GTCCACATAT TGATGAGAAC TGTGGAGACT CTATACACCC AGAATGCTCT 
GGGGTAGAAC TCGGTAAGAG CGCTGGTGAT TCCAGGACCC AGGGGGAGCC CTCTGAGCGG 
GAGATCACCA GTGTTGCTGG GAAACGAACT TGTTCGGACT CAGGAGACGA GGATGATTCT 
CCATCGGAGA AGAGGGCGAA GACGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071020.1
CDS	174..2543
Translation	MKEARGCSMRSAVGFLSRRTIDGHPQLKEEFQRRRLSGCSSLYKKDMLGHFGCVNAIEFSNNGGQWLVSGGDDRRVLLWHMEKAIHSRAKPMKLKGEHLSNIFCLAFDSTNKRVFSGGNDEQVILHDVERGETLNVFLHDDAVYGLSVSPVNDNVFASSSDDGRVLIWDTREPPHGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIRKPRSSLLRYGGSLSLQSAMSVRFNSTGTQLLALRRRLPPVLYELHSRLPSFQFDNQGYFNSCTMKSCCFAGDRDQYILSGSDDFNLYMWRIPTDPEAGGAGRVVNGAFMVLKGHRSIVNQVRFNPHTYMICSSGVEKVIKVWSPYKQPDSLGDLEGLVEDKSRSLYTHEEYISLVLNSGTGLSHDYVSQSVQEDPRMMAFFDSLVRREIEGWSSDSDSDLSEGAILQLHARARRSTRSTRSTPRSPAAVSAAHHSDSEHSSSSSLAGPDVSGSERTDQDRSGSQRNRARPRASAFLLDLVNEDSDSSGFWLDPMPRPRSPSPRENSSLSSHASSAGVASSSSSSPSSSSSSSSSTDGVDEELRRSRMRQRNAARWRRHIGFSPRDSAQTLHSATDSSNYPNISIHDQSSPSSSSGDEDRPFRNRGKQKPSPSHLSDRQSQSRCRRRITSSFSGAGDGPLERAKSRAGFLAESGEELERGRTSGEELSTVEDLNLTLSSRTTVAGVNGRPHIDENCGDSIHPECSGVELGKSAGDSRTQGEPSEREITSVAGKRTCSDSGDEDDSPSEKRAKTW

Target ORF information:

RefSeq Version	NM_001077552.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ddb1 and cul4 associated factor 5 (dcaf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077552.1

ORF Insert Sequence:

ATGAAGGAGG CGCGGGGCTG CAGCATGCGG AGCGCCGTCG GGTTTCTCTC CCGCAGGACG 
ATCGACGGAC ACCCGCAGCT GAAGGAGGAG TTCCAGCGCC GCAGACTGTC CGGCTGCTCT 
AGCCTCTACA AGAAGGACAT GCTCGGACAC TTCGGCTGCG TCAACGCCAT CGAGTTCTCC 
AACAATGGAG GCCAGTGGCT GGTGTCCGGG GGAGACGACC GCAGGGTGTT GCTGTGGCAC 
ATGGAGAAAG CAATACACTC ACGGGCAAAA CCAATGAAGC TGAAGGGGGA GCACCTGTCC 
AACATCTTCT GCCTGGCGTT TGACAGCACC AACAAGCGTG TTTTCTCAGG AGGAAATGAT 
GAGCAGGTGA TTCTGCATGA CGTGGAACGT GGGGAGACGC TCAATGTCTT CTTGCATGAT 
GACGCCGTGT ATGGTCTGTC CGTCAGTCCG GTTAATGATA ACGTCTTCGC CAGCTCCTCT 
GATGACGGAA GAGTTCTCAT ATGGGATACC AGAGAGCCAC CACATGGAGA ACCGTTCTGC 
TTGGCCAACT ACCCCTCAGC ATTTCACAGT GTGATGTTTA ACCCTGTTGA GCCTCGTCTG 
CTGGCCACTG CAAACTCTAA AGAGGGAGTT GGACTATGGG ATATCCGCAA ACCCCGCAGT 
TCTCTGTTGC GGTACGGAGG CAGTTTGTCT CTTCAGAGTG CCATGAGTGT TCGCTTTAAC 
AGCACGGGGA CGCAGCTGCT GGCTCTGCGC AGACGTCTGC CACCCGTCCT CTATGAGCTC 
CACTCTCGTC TGCCCAGCTT CCAGTTTGAT AACCAGGGCT ACTTCAACTC CTGCACTATG 
AAGAGCTGCT GTTTCGCTGG CGACCGAGAT CAGTACATCT TGTCTGGCTC TGATGATTTT 
AACCTTTACA TGTGGAGAAT TCCCACTGAC CCTGAAGCAG GAGGCGCGGG TCGAGTGGTG 
AATGGGGCCT TTATGGTTTT AAAAGGTCAT CGGTCCATTG TGAATCAGGT CCGCTTTAAC 
CCTCACACGT ACATGATCTG CTCCTCCGGG GTGGAGAAGG TCATCAAGGT TTGGAGTCCG 
TATAAGCAGC CAGACAGTTT AGGAGATCTA GAAGGCCTGG TGGAAGACAA GTCTCGCTCT 
CTCTACACTC ATGAGGAATA CATAAGCCTG GTACTCAACA GCGGGACCGG CCTGTCACAC 
GATTATGTCA GCCAGTCAGT GCAGGAAGAC CCTCGAATGA TGGCCTTTTT TGACTCACTG 
GTTCGGCGTG AGATCGAGGG CTGGAGCTCG GACTCTGACA GCGATTTGAG CGAGGGTGCG 
ATTCTGCAGC TGCATGCCAG AGCCCGGCGG TCGACCCGCT CCACACGATC CACACCACGA 
TCTCCTGCTG CTGTGTCTGC CGCTCATCAT AGCGACTCCG AACACTCGTC TTCATCTTCT 
CTAGCGGGAC CTGATGTGTC CGGGAGCGAA CGGACGGATC AGGATAGAAG TGGGTCACAG 
AGGAACCGGG CCAGACCACG TGCGTCTGCG TTTCTGCTTG ATCTTGTGAA CGAGGATTCA 
GATTCCAGTG GGTTTTGGTT AGACCCGATG CCCCGTCCCA GATCCCCTAG CCCACGTGAA 
AACTCAAGTC TTTCTAGCCA CGCCTCCTCA GCGGGCGTAG CCTCCAGTTC AAGCTCCTCC 
CCCAGCTCAT CTTCTAGCTC CTCCTCCAGC ACTGATGGAG TTGACGAGGA GCTGCGCAGG 
AGCAGGATGC GACAGCGCAA TGCAGCCAGA TGGCGCCGCC ATATTGGCTT TTCTCCAAGA 
GACTCGGCCC AAACACTTCA CAGCGCCACA GACTCCTCCA ACTATCCCAA CATTTCCATC 
CACGATCAGT CGTCCCCCTC TTCGTCCTCA GGTGACGAAG ATCGGCCTTT CAGAAATCGT 
GGAAAACAAA AGCCAAGTCC GTCGCACTTA TCTGACAGGC AATCGCAGTC CAGATGTAGG 
CGCCGCATCA CTTCTTCCTT CTCAGGGGCT GGGGACGGCC CTTTGGAGAG GGCGAAATCC 
CGAGCAGGCT TTCTGGCGGA GTCTGGGGAG GAACTGGAGC GAGGGAGAAC TAGCGGAGAA 
GAGTTGAGTA CTGTGGAGGA TTTAAACCTG ACACTCAGCA GTCGGACCAC AGTGGCTGGT 
GTTAACGGAC GTCCACATAT TGATGAGAAC TGTGGAGACT CTATACACCC AGAATGCTCT 
GGGGTAGAAC TCGGTAAGAG CGCTGGTGAT TCCAGGACCC AGGGGGAGCC CTCTGAGCGG 
GAGATCACCA GTGTTGCTGG GAAACGAACT TGTTCGGACT CAGGAGACGA GGATGATTCT 
CCATCGGAGA AGAGGGCGAA GACGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates.
Developmental dynamics : an official publication of the American Association of Anatomists238(11)2936-47(2009 Nov)
Holterhoff CK,Saunders RH,Brito EE,Wagner DS

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dcaf5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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