top3a cDNA ORF clone, Danio rerio(zebrafish)

The following top3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa08651 XM_683603.9
Latest version!
Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
ODa08651 XM_683603.8 Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa08651
    Clone ID Related Accession (Same CDS sequence) XM_683603.8 , XM_683603.9
    Accession Version XM_683603.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product DNA topoisomerase 3-alpha
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683603.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
    TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC
    GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC
    TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT
    GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG
    AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT
    GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG
    ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC
    TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA
    AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG
    GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT
    TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC
    TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC
    CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA
    TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG
    TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG
    CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA
    AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT
    CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA
    CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC
    ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC
    ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG
    GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA
    GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT
    TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC
    ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT
    TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT
    GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA
    GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA
    GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA
    GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG
    AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA
    GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC
    CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA
    TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC
    GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG
    AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT
    GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC
    CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA
    CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT
    GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC
    GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT
    AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT
    GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT
    GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT
    ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG
    GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG
    GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT
    GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC
    TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_688695.4
    CDS650..3646
    Translation

    Target ORF information:

    RefSeq Version XM_683603.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_683603.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
    TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC
    GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC
    TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT
    GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG
    AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT
    GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG
    ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC
    TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA
    AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG
    GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT
    TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC
    TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC
    CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA
    TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG
    TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG
    CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA
    AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT
    CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA
    CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC
    ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC
    ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG
    GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA
    GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT
    TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC
    ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT
    TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT
    GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA
    GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA
    GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA
    GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG
    AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA
    GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC
    CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA
    TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC
    GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG
    AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT
    GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC
    CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA
    CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT
    GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC
    GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT
    AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT
    GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT
    GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT
    ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG
    GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG
    GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT
    GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC
    TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa08651
    Clone ID Related Accession (Same CDS sequence) XM_683603.8 , XM_683603.9
    Accession Version XM_683603.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product DNA topoisomerase 3-alpha
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683603.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
    TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC
    GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC
    TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT
    GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG
    AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT
    GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG
    ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC
    TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA
    AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG
    GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT
    TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC
    TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC
    CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA
    TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG
    TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG
    CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA
    AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT
    CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA
    CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC
    ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC
    ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG
    GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA
    GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT
    TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC
    ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT
    TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT
    GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA
    GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA
    GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA
    GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG
    AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA
    GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC
    CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA
    TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC
    GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG
    AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT
    GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC
    CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA
    CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT
    GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC
    GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT
    AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT
    GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT
    GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT
    ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG
    GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG
    GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT
    GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC
    TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_688695.4
    CDS24..3020
    Translation

    Target ORF information:

    RefSeq Version XM_683603.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio topoisomerase (DNA) III alpha (top3a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_683603.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACGTTT TGTTTAGGGC ACTAAGAAAA ACTGTCAACA GTCGATTTTA TAGACATATA 
    TCTGTAAGCA TGATAAGACG CACTCAGATT AAGAAAGTGC TGTGTGTGGC CGAGAAAAAC
    GATGCAGCTA AAGGCATAGC AGAGATCATG TCAAATGGCA GATCCAGAAG AAGAGAAGGC
    TGCTCTGTTT ACAACAAAAT ATATGAGTAT GAATACAACC TGTTCGGCCA AAATGTTACT
    GTAAACATGA CGTCAGTATC AGGGCATCTC TTGGCTCTTG AATTCAAAGC TCCTTTTCAG
    AAATGGCATA GTTGTAGTCC AGTCCTATTG TTTGATGCTG AGGTGGAAAA GTACTGCCCT
    GAGAACTTTA TACCGATTAA GAGGACTTTG GAGAGAGAGG TGAGGCAGTG TCAAGCTCTG
    ATTGTTTGGA CTGATTGTGA CAGGGAAGGA GAGAATATCG GCTTCGAGAT CATTGATGTC
    TGCAAAGCAG TGAAGCCAAA CATCCAGGTC TTCCGTGCAC GGTTCTCTGA GATCACCCCA
    AACTCCATTA GGAGAGCCTG TGAGACCCTC ACGGAGCCTG ATATCAATGT TAGCGATGCG
    GTCGACGTGA GACAGGAGCT AGATCTTCGC ATTGGGGCAT CGTTTACCAG ATTCCAGACT
    TTGCGCCTGC AGAAGATATT CCCAGAATCT CTCTCTGATC AGCTCATCAG TTATGGCAGC
    TGTCAGTTCC CCACTCTTGG CTTTGTGGTA GAACGATTTA AAGCCATTCA AGCCTTCATC
    CCTGAAACAT TCTTCAAAAT CAAAGTTGTC CATGAACCTA ATGAAGAGGA GTCAGTGGAA
    TTCAACTGGA AAAGACATCG ACTGTTCAAT CACACCGCAT GCCTTGTGCT GTATCAGATG
    TGCATGGAGG AACCCATGGC GAAAGTTATA TCTGTAACAA GTAAACCAAA AAGCAAATGG
    CGACCTTTAC CACTGGACAC TGTGGAACTT GAAAAACTGG CCTCCAGAAA ATTGAGGATA
    AGTGCCAAAG AAACCATGAA GATTGCAGAA AAGCTGTATA CTCAAGGTTT TATCAGTTAT
    CCTCGCACGG AGACCAACAT GTTTCCACAG AACCTGAACC TTACCCGTCT GGTTGAGCAA
    CAGACACAGG ACCAAGAATG GGGAAATTTT GCCCAGCGTA TTTTGGAAAG CGGTGGGCCC
    ACTCCTCGAA ACGGAAACAA ATCAGATCAG GCCCATCCCC CCATTCACCC CACCAAATAC
    ACCAACGGTT TACAGGGAAA TGAAAAGAGA CTTTATGAGT TCATTGTACG TCACTTCCTG
    GCCTGTTGCT CCAAAGATGC TCAGGGTCAA GAGGTCACAG TCGAAATTGA TATAGCGGAA
    GAGAGATTCT CTGCTAGCGG ACTGACGATC ATCGCTCGCA ATTATCTGGA AGTGTACCCT
    TATGACAAGT GGTACAATAA GGTGATTCCA TCATACAGCC CTAACACCAC ATTCCAGCCC
    ACAGCGATTG AGATGGTGGA AGGACAGACC AGTCCACCAC AGCTTCTGAC AGAGGCAGAT
    TTGATATCAC TCATGGAGAA ACATGGCATT GGTACCGATG CAACACATGC TGAACACATT
    GAGACCATTA AGAGTCGCAT GTATGTGGGG CTGACGGCAG ACCAGAGGTT CCTTCCCGGA
    GAGCTCGGCA TGGGTCTCGT TGAAGGATAT AATTCTATGG GATACGAGAT GTCTAAACCA
    GACCTGAGAG CTGAGCTGGA GGCAGACCTT AAACTTGTGT CAGAGGGCCG GAAGAATAAA
    GCATCTGTCC TCAGTTATCA TGTGGCAAAA TACAAAGCCG TTTTCATCGA GTCTGTGAGG
    AAGGCAAAGA AATTAGATGA GGCTTTGTCC AATTATCTGG GTCCGGCTCA GGAGATTGCA
    GAACAGGAGC AAGTCGAGTT GGAGATACCA TTACCTGTGC GAAAGTGTCC TCAGTGTAAC
    CGAGACATGG TGCTGAAGAA GAAGAAAGAC AGCACAGGGA TGCTTCTTTC ATGCTTGGGA
    TATCCAGCGT GTAAAGCAGC CGTGTGGTTT CCTGACACTG TCCTGGAAGT CAGCAGAGAC
    GAGAGCATCT GTCCAACTTG CCGTCCACAT CCGGTTCATA TGCTGAAGTT TAAATTCAAG
    AGGGGAAGTT TGCCACCCAT GATGCCTCTT GAATTTGTTG GATGCATCGG AGGATGTGAT
    GACACTCTCA GAGAGGTTTT AAACTTGAAA TATCTCAGAG GACGTGATGC AGAATCTGCC
    CCTCAGGCCA ATAACAGCTC CAGATCCAGT CAACACCACA TTCATAGAAC CAGCTCGGCA
    CCTCCTCCAT CCAGGGTAGA GAACATTAGA CCTCCAGCAC CCAGACCTCG AGCTGATCCT
    GGTCTGCAAC CTCCACCTTC AACTCAGGGC GGTGCCATTG TGTGTAACTG TGGTCAGGAC
    GCCTTACTTC TCACCGTGCG CAAAGAGGGT CCCAATCAAG GGCGTCAGTT TTACAAGTGT
    AACACAGGAG ACTGCAAGTT CTTCCTGTGG GCCGATCAGC CACCTGAACA GGGGGTGCCT
    GAAAGAAACA GAGGGCCTTT AAGTCAAAAC GTCCAGCCTC CCAGGCCTTC CAATGGGTTT
    GGGAATGTCT CACAGCAGAG GCAGAACCAC AGAGGGTCTG GAGGCGATGA AGGGGAAACT
    ATGTGTAACT GTAATGAGCC TTCAGTCACC AGGACTGTCA TGAAGGAAGG GCCGAACAAG
    GGCAGAATGT TTCACACCTG CGGGAAACCA CGAGATCAGC AGTGTGGCTT CTTCCAGTGG
    GCCAATGAAA ATGTGGCACC AGCCACAGCG TTTTCTGGAG GAAATGGTGG TAAACCATCT
    GGGAACAAAA AGGCCAGTAC TACCTCAGTC AAGCCTCCCA CTGCCAAAAG GCAGAGGACC
    TGTGGAATAT GTCATGAGCC AGGACACACC AGAGTCACCT GTCCTCAGGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.