gsk3aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gsk3aa
Entrez Gene ID	560194
Full Name	glycogen synthase kinase 3 alpha a
Synonyms	si:ch211-39e15.2,zgc:158379
General protein information	Preferred Names glycogen synthase kinase 3 alpha a Names glycogen synthase kinase 3 alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044921.3	NP_001038386.2	glycogen synthase kinase 3 alpha isoform 1
NM_001365534.1	NP_001352463.1	glycogen synthase kinase 3 alpha isoform 2
NM_001044921.2	NP_001038386.2	glycogen synthase kinase 3 alpha
XM_009294003.2	XP_009292278.1	glycogen synthase kinase 3 alpha isoform X1
XM_009294003.3	XP_009292278.1	glycogen synthase kinase 3 alpha isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GSK3A	NP_001095662.1
Eremothecium gossypii	AGOS_ADR253W	NP_984349.2
Magnaporthe oryzae (rice blast fungus)	MGG_12122	XP_003719885.1
Neurospora crassa	NCU04185	XP_961145.1
Arabidopsis thaliana (thale cress)	ATSK12	NP_187235.1
Oryza sativa (rice)	Os01g0252100	NP_001042602.1
Pan troglodytes (chimpanzee)	GSK3A	XP_530044.2
	GSK3A	XP_001104964.1
Mus musculus (house mouse)	Gsk3a	NP_001026837.1
Arabidopsis thaliana (thale cress)	SK 11	NP_568486.1
Homo sapiens (human)	GSK3A	NP_063937.2
Rattus norvegicus (Norway rat)	Gsk3a	NP_059040.1
Danio rerio (zebrafish)	gsk3aa	NP_001038386.2
Danio rerio (zebrafish)	gsk3ab	NP_571465.1
Kluyveromyces lactis	KLLA0F17006g	XP_455844.1
Schizosaccharomyces pombe (fission yeast)	gsk3	NP_593134.1
Xenopus tropicalis (tropical clawed frog)	gsk3a	XP_002936450.1
Canis lupus familiaris (dog)	GSK3A	XP_005616585.1
Saccharomyces cerevisiae (baker's yeast)	RIM11	NP_013859.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-34a Regulates Sperm Motility in Zebrafish.
Guo W, Xie B, Xiong S, Liang X, Gui JF, Mei J
International journal of molecular sciences18(12)(2017 Dec)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Dynamics of zebrafish fin regeneration using a pulsed SILAC approach.
Nolte H, H?lper S, Housley MP, Islam S, Piller T, Konzer A, Stainier DY, Braun T, Kr?ger M
Proteomics15(4)739-51(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gsk3aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gsk3aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32587	NM_001044921.3 Latest version!	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa43247	NM_001365534.1 Latest version!	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa43247	XM_009294003.3 Latest version!	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa32587	NM_001044921.2	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa43247	XM_009294003.2	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gsk3aa ( NM_001044921.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038386.2 Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa32587
Clone ID Related Accession (Same CDS sequence)	NM_001044921.3 , NM_001044921.2
Accession Version	NM_001044921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase 3 alpha isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004874.11. On Sep 1, 2018 this sequence version replaced NM_001044921.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01012105.1, BC129215.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAG 
CTGATGATTC AGCCGCAGCT CAATTCCACT CTCATTCCAC CGCATGCCCG CGCACAAACC 
GTAGCATCAT CCAACGATGG CTCTGAAGGC TCCGTACAGC CCGGATCTAC CCTGACCAAC 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038386.2
CDS	141..1529
Translation	MFHPAPGQVDEGSADRRPTASVNFQSDMSGSGRPRTSSFAEPQGASGTAAASAGTAAVVGSNAGKPGVSQASADSSSACANLKLARDSGKVTTVVATPGQGPDRPQEVSYTDIKVIGNGSFGVVYQARLIDTHEWVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSNIDIWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKPRTPPEAIALCSRLLEYTPVTRLSPLQACAHAFFDELRQPGTRLPSGRELPPLFNFTTTELMIQPQLNSTLIPPHARAQTVASSNDGSEGSVQPGSTLTNST

Target ORF information:

RefSeq Version	NM_001044921.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044921.3

ORF Insert Sequence:

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAG 
CTGATGATTC AGCCGCAGCT CAATTCCACT CTCATTCCAC CGCATGCCCG CGCACAAACC 
GTAGCATCAT CCAACGATGG CTCTGAAGGC TCCGTACAGC CCGGATCTAC CCTGACCAAC 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3aa ( NM_001044921.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038386.2 Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), mRNA.

Price & Availability↑

CloneID	ODa32587
Clone ID Related Accession (Same CDS sequence)	NM_001044921.3 , NM_001044921.2
Accession Version	NM_001044921.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase 3 alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX004874.11. On Oct 5, 2007 this sequence version replaced NM_001044921.1. ##Evidence-Data-START## Transcript exon combination :: BC129215.1, GFIL01012105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAG 
CTGATGATTC AGCCGCAGCT CAATTCCACT CTCATTCCAC CGCATGCCCG CGCACAAACC 
GTAGCATCAT CCAACGATGG CTCTGAAGGC TCCGTACAGC CCGGATCTAC CCTGACCAAC 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038386.2
CDS	97..1485
Translation	MFHPAPGQVDEGSADRRPTASVNFQSDMSGSGRPRTSSFAEPQGASGTAAASAGTAAVVGSNAGKPGVSQASADSSSACANLKLARDSGKVTTVVATPGQGPDRPQEVSYTDIKVIGNGSFGVVYQARLIDTHEWVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSNIDIWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKPRTPPEAIALCSRLLEYTPVTRLSPLQACAHAFFDELRQPGTRLPSGRELPPLFNFTTTELMIQPQLNSTLIPPHARAQTVASSNDGSEGSVQPGSTLTNST

Target ORF information:

RefSeq Version	NM_001044921.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044921.2

ORF Insert Sequence:

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAG 
CTGATGATTC AGCCGCAGCT CAATTCCACT CTCATTCCAC CGCATGCCCG CGCACAAACC 
GTAGCATCAT CCAACGATGG CTCTGAAGGC TCCGTACAGC CCGGATCTAC CCTGACCAAC 
AGCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3aa ( NM_001365534.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352463.1 Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa43247
Clone ID Related Accession (Same CDS sequence)	NM_001365534.1 , XM_009294003.2 , XM_009294003.3
Accession Version	NM_001365534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase 3 alpha isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004874.11. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352463.1
CDS	141..1451
Translation	MFHPAPGQVDEGSADRRPTASVNFQSDMSGSGRPRTSSFAEPQGASGTAAASAGTAAVVGSNAGKPGVSQASADSSSACANLKLARDSGKVTTVVATPGQGPDRPQEVSYTDIKVIGNGSFGVVYQARLIDTHEWVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSNIDIWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKPRTPPEAIALCSRLLEYTPVTRLSPLQACAHAFFDELRQPGTRLPSGRELPPLFNFTTTDGSEGSVQPGSTLTNST

Target ORF information:

RefSeq Version	NM_001365534.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365534.1

ORF Insert Sequence:

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3aa ( XM_009294003.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292278.1 Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.

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CloneID	ODa43247
Clone ID Related Accession (Same CDS sequence)	NM_001365534.1 , XM_009294003.2 , XM_009294003.3
Accession Version	XM_009294003.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycogen synthase kinase 3 alpha isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292278.1
CDS	111..1421
Translation	MFHPAPGQVDEGSADRRPTASVNFQSDMSGSGRPRTSSFAEPQGASGTAAASAGTAAVVGSNAGKPGVSQASADSSSACANLKLARDSGKVTTVVATPGQGPDRPQEVSYTDIKVIGNGSFGVVYQARLIDTHEWVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSNIDIWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKPRTPPEAIALCSRLLEYTPVTRLSPLQACAHAFFDELRQPGTRLPSGRELPPLFNFTTTDGSEGSVQPGSTLTNST

Target ORF information:

RefSeq Version	XM_009294003.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294003.2

ORF Insert Sequence:

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gsk3aa ( XM_009294003.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292278.1 Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43247
Clone ID Related Accession (Same CDS sequence)	NM_001365534.1 , XM_009294003.2 , XM_009294003.3
Accession Version	XM_009294003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycogen synthase kinase 3 alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292278.1
CDS	113..1423
Translation	MFHPAPGQVDEGSADRRPTASVNFQSDMSGSGRPRTSSFAEPQGASGTAAASAGTAAVVGSNAGKPGVSQASADSSSACANLKLARDSGKVTTVVATPGQGPDRPQEVSYTDIKVIGNGSFGVVYQARLIDTHEWVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYCSGEKKDEVYLNLVLDYVPETVYRVARHFSKAKTIIPIFYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSNIDIWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKPRTPPEAIALCSRLLEYTPVTRLSPLQACAHAFFDELRQPGTRLPSGRELPPLFNFTTTDGSEGSVQPGSTLTNST

Target ORF information:

RefSeq Version	XM_009294003.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glycogen synthase kinase 3 alpha a (gsk3aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294003.3

ORF Insert Sequence:

ATGTTTCACC CAGCACCAGG CCAAGTAGAC GAGGGAAGCG CTGATCGTCG ACCCACCGCC 
TCGGTAAACT TTCAGTCAGA TATGAGCGGC AGCGGGCGGC CCAGGACAAG CTCGTTCGCT 
GAGCCTCAGG GGGCGTCCGG AACCGCTGCT GCATCCGCCG GAACCGCCGC TGTCGTGGGG 
AGCAACGCAG GAAAGCCCGG GGTCTCGCAG GCCTCGGCGG ACAGTTCATC TGCCTGCGCG 
AATTTAAAAC TAGCTAGAGA CAGTGGGAAG GTGACCACAG TGGTTGCCAC CCCAGGCCAG 
GGCCCGGATC GCCCGCAGGA AGTGTCCTAC ACTGATATCA AGGTGATCGG TAATGGCTCC 
TTTGGCGTGG TTTATCAGGC ACGTCTCATT GACACTCATG AGTGGGTGGC CATCAAGAAG 
GTTCTTCAGG ACAAGCGATT TAAGAACCGA GAACTTCAGA TCATGAGAAA ACTGGACCAC 
TGCAATATAG TGAGGCTGCG CTATTTCTTC TATTGCAGCG GAGAGAAGAA GGATGAGGTT 
TACCTCAATC TAGTGCTGGA CTACGTGCCT GAGACTGTCT ACAGGGTGGC TCGACACTTT 
AGCAAGGCTA AAACCATTAT TCCCATCTTT TATGTGAAGG TGTACATGTA TCAGCTTTTT 
CGCAGTTTGG CCTACATCCA TTCCCAGGGC GTATGTCACC GAGACATCAA ACCACAGAAC 
CTTCTGGTGG ACCCAGAAAC AGCTGTTCTC AAGCTCTGTG ACTTTGGCAG TGCAAAGCAG 
CTGGTGAGGG GTGAGCCTAA CGTCTCTTAT ATCTGCTCGC GATACTATCG CGCCCCTGAG 
CTTATATTTG GTGCCACGGA CTACACCTCC AACATTGACA TTTGGTCAGC TGGATGTGTG 
CTGGCCGAAC TGCTGCTGGG ACAGCCCATC TTCCCTGGAG ACAGTGGTGT GGACCAGCTA 
GTGGAGATCA TAAAGGTACT TGGAACCCCC ACAAGAGAAC AGATCCGTGA AATGAACCCA 
AACTACACAG AGTTCAAATT CCCACAGATC AAAGCTCATC CATGGACCAA GGTGTTTAAA 
CCCAGAACTC CTCCTGAGGC GATCGCGCTG TGCTCTCGGC TGTTGGAGTA TACGCCAGTG 
ACTCGGCTGT CTCCGCTTCA GGCTTGTGCT CATGCCTTCT TTGACGAGTT GCGCCAACCT 
GGCACCCGTT TGCCCAGCGG CCGAGAGCTG CCTCCGCTTT TCAACTTCAC CACAACAGAT 
GGCTCTGAAG GCTCCGTACA GCCCGGATCT ACCCTGACCA ACAGCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-34a Regulates Sperm Motility in Zebrafish.
International journal of molecular sciences18(12)(2017 Dec)
Guo W,Xie B,Xiong S,Liang X,Gui JF,Mei J

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Dynamics of zebrafish fin regeneration using a pulsed SILAC approach.
Proteomics15(4)739-51(2015 Feb)
Nolte H,H?lper S,Housley MP,Islam S,Piller T,Konzer A,Stainier DY,Braun T,Kr?ger M

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gsk3aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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