dot1l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dot1l
Entrez Gene ID	559945
Full Name	DOT1-like histone H3K79 methyltransferase
Synonyms	si:dkey-211f22.1
General protein information	Preferred Names DOT1-like histone H3K79 methyltransferase Names histone-lysine N-methyltransferase, H3 lysine-79 specific
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170535.3	XP_005170592.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
XM_017353402.1	XP_017208891.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
XM_009296351.2	XP_009294626.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
XM_009296352.2	XP_009294627.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_005170534.4	XP_005170591.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_009296352.3	XP_009294627.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
XM_005170534.3	XP_005170591.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
XM_009296351.3	XP_009294626.1	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DOT1L	XP_003953365.1
Canis lupus familiaris (dog)	DOT1L	XP_005633296.1
Homo sapiens (human)	DOT1L	NP_115871.1
	DOT1L	XP_001097213.2
Xenopus tropicalis (tropical clawed frog)	dot1l	XP_002937984.2
Bos taurus (cattle)	DOT1L	XP_005209126.1
Mus musculus (house mouse)	Dot1l	NP_955354.1
Rattus norvegicus (Norway rat)	Dot1l	NP_001102203.1
Gallus gallus (chicken)	DOT1L	XP_004948823.1
Danio rerio (zebrafish)	dot1l	XP_005170590.1

Gene Ontology

The following dot1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dot1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26970	XM_005170535.3 Latest version!	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa57246	XM_017353402.1 Latest version!	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa26968	XM_005170534.4 Latest version!	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43204	XM_009296351.3 Latest version!	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43205	XM_009296352.3 Latest version!	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa43205	XM_009296352.2	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa26968	XM_005170534.3	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43204	XM_009296351.2	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dot1l ( XM_005170535.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170592.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa26970
Clone ID Related Accession (Same CDS sequence)	XM_005170535.3
Accession Version	XM_005170535.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGCCGA TAAAGAAAAG AGCCCGACTG CACCCGGCGA CACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170592.1
CDS	254..2641
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRPIKKRARLHPATL

Target ORF information:

RefSeq Version	XM_005170535.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170535.3

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGCCGA TAAAGAAAAG AGCCCGACTG CACCCGGCGA CACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_017353402.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208891.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa57246
Clone ID Related Accession (Same CDS sequence)	XM_017353402.1
Accession Version	XM_017353402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC CACTACCTGC CCATTTCTCC 
AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC AGGCAGCTTG GGCAACCCCT 
GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC CGCAGGCACC TCAACCAGGA 
TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT CGGCCGATAA AGAAAAGAGC 
CCGACTGCAC CCGGCGACAC TATAACCAGT CTACCAATCA GCATCCCTCT CAGCACTGTG 
CATCCCAGCA AACTTCCTGT CAGCATCCCT CTGGCCAGTG TGGTGCTGCC GAACCGCGTC 
GAAAGACTGA GGAGCACTCT TAGTCCGGTA TTGAATCCAG CAGGACAGAG CAATGGCAAG 
CCACCTCTCA CGCCTACCTC AGGGTACTCC TCCAGCTCAG GATTGGTTAA TGGGTCTCAC 
TCCACCATGC TGACCGAGGA CCAAGACTGT GATGTTGCCT CCCCTCCTCC TCACAGCACT 
CCCGTCATGG GTCTTCAGCC CCGCGGCTCT GGCCTCAGTC CTCCACTGGG CACCGGAGGC 
GTTCTCCAGT ACGCCGACGG TCCACCAAGG CTTCCCCCCG AGGATGGCCA TGAGCGCCAA 
GGCCCCGAAT CCGACACAGA GCCCTTAGAT AGTGAGGCTC GCCGGCGCAT TTTTTTCTCT 
TCATCCTCCT CTTCTTCCTC ATCCTCATCC TCCACTGGAG GCTCGAGGCT GCACCATGGG 
TCTGTAAAGC AGGGTCACCA TCATCACAGT AAGCACCATC ACCACCACCA CCACCATCAT 
CACCATCACT CTCCAAGCTC CCACAGCCAA GAGGGCCGAA AACGAGGAAG ACGAAAGCGC 
AGCTCATCTG GAGCCGTGCC CATGAGTGGA TCCCCCAAAA GAAGGGTCTT TCCTGGACTG 
GGCTGCTCAA GTCATTCCTC TGGATCACCG CTTAATATCA ATTCAATGGT AAACAACATA 
AACCAGCCTT TGGAGATCTC CGCCATTTCT TCCCCAGAGC AGTCAGGCAG AAGCCCATGT 
GAGCAAGATC TGGATCAGCC ACCTGTTTTG AAACGAGAGC GTCCTCTTGA GCTCAACAAC 
ACCGGCCGTT ATTCCTCTGC CCCAAGTTCA GATGAAGAAG GCAATGGTTA CGCCCCGGAC 
ACCTCTAGTT CAAGCCGAAT TGAACGAAAA ATTGCTACTA TTTCCTTAGA CCGAGATGGA 
CCAGTTAGAG ACTTGGAAAG AGGTTTACAG GGACGTAAAT CAGGTGCCAG CAGCGTGAGC 
AGCGAGGTAT CCTCTTCCTC CGGAACCAGC AAGTGGAAAT CCACCTTCTC CCCCATTTCT 
GATCCCAAGC CAACTCCTCC AGACATCCGC CAAGGTGGCT CACCATTTAG CGTTGGAGCT 
TCCCGAGGAG GCACAGACTC AGACTCGGAC CACAAACCCC AGAGGAGAGG GGAGCGTGAG 
CGTGAGCCTT ATGGAAATTC AAATCTCTTC CTCAGTCAGG ATGGCAGTGG TCGCTCGGCG 
GTAAACACGT CAGAGCGTTC GCTGCAGAAA CAGAAAGTGC GGGAGTGGGA CCTGAAATCT 
GTAAGTGGAT TGGCCAGTCA AAACCTATTT ATATCTGCAG CAGCCAGTGG AGGCATCCTG 
AGTGGGAAAG TGGGCGGGGC TGCCACTGTG TCATCCGCCT CTGCGGTAGG GCAATATTTC 
CCACTGGGCG GAGCTTCTGT TCTACAACCC TTATTTGGAA CTCAGGCAGC GGGACCAGCC 
ACCAGCGGAG CCCCCCGATT GGTCAACGGA CATTCGGCGC TTGGCAGTTT CTCCAGTGCC 
GGACTGGCAG GGGGCGCTGC AGGAGGGATT TTTCACCACA TGGTGTCTTC AAGCTCCTCA 
CAGCAGTCTG GGCCCAATCT CCCAAGCTCC CATAATTCCC AACAGAGGCA TCTGGACACG 
AACCCCAAAG CAGGGGCCTC GTTCTTGGCC TCTGGCCCTA ACCGCACTCA AACGGTCCTA 
CACGCACCCT CTCCACCCAC GCTCGCTCTT CCCCCGCCCC CACCCCTCCT GAACACTGTC 
CCGTCCCAAC CGTCCTCTCC GCGCTCATCC AGACCGCAGT CGTCCATCCC CGTGGCCACC 
TCGTCCCAAT CCCTGGCTGC CGCGTCCTCT TCCTGCACCT CATCCGCTTC CTCAAGGCCC 
TTCACTGTCC ACTATCCTCC GCATCTGCAG CCTCCGGGCA GCGCTGGTTC AGGTGGGACG 
GGGGCCTCCT GGAGGACTCT GGCATCCTAT ACGGCCTCGC AGATTCCTGG GAATCGGCCC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208891.1
CDS	317..2662
Translation	MHRKCNGQKRTPHPLPAHFSRPRNCAANPGLQNQAAWATPARLHPLFSSKDRPPQAPQPGFSRKPVPGTRKHPSADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR

Target ORF information:

RefSeq Version	XM_017353402.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353402.1

ORF Insert Sequence:

ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC CACTACCTGC CCATTTCTCC 
AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC AGGCAGCTTG GGCAACCCCT 
GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC CGCAGGCACC TCAACCAGGA 
TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT CGGCCGATAA AGAAAAGAGC 
CCGACTGCAC CCGGCGACAC TATAACCAGT CTACCAATCA GCATCCCTCT CAGCACTGTG 
CATCCCAGCA AACTTCCTGT CAGCATCCCT CTGGCCAGTG TGGTGCTGCC GAACCGCGTC 
GAAAGACTGA GGAGCACTCT TAGTCCGGTA TTGAATCCAG CAGGACAGAG CAATGGCAAG 
CCACCTCTCA CGCCTACCTC AGGGTACTCC TCCAGCTCAG GATTGGTTAA TGGGTCTCAC 
TCCACCATGC TGACCGAGGA CCAAGACTGT GATGTTGCCT CCCCTCCTCC TCACAGCACT 
CCCGTCATGG GTCTTCAGCC CCGCGGCTCT GGCCTCAGTC CTCCACTGGG CACCGGAGGC 
GTTCTCCAGT ACGCCGACGG TCCACCAAGG CTTCCCCCCG AGGATGGCCA TGAGCGCCAA 
GGCCCCGAAT CCGACACAGA GCCCTTAGAT AGTGAGGCTC GCCGGCGCAT TTTTTTCTCT 
TCATCCTCCT CTTCTTCCTC ATCCTCATCC TCCACTGGAG GCTCGAGGCT GCACCATGGG 
TCTGTAAAGC AGGGTCACCA TCATCACAGT AAGCACCATC ACCACCACCA CCACCATCAT 
CACCATCACT CTCCAAGCTC CCACAGCCAA GAGGGCCGAA AACGAGGAAG ACGAAAGCGC 
AGCTCATCTG GAGCCGTGCC CATGAGTGGA TCCCCCAAAA GAAGGGTCTT TCCTGGACTG 
GGCTGCTCAA GTCATTCCTC TGGATCACCG CTTAATATCA ATTCAATGGT AAACAACATA 
AACCAGCCTT TGGAGATCTC CGCCATTTCT TCCCCAGAGC AGTCAGGCAG AAGCCCATGT 
GAGCAAGATC TGGATCAGCC ACCTGTTTTG AAACGAGAGC GTCCTCTTGA GCTCAACAAC 
ACCGGCCGTT ATTCCTCTGC CCCAAGTTCA GATGAAGAAG GCAATGGTTA CGCCCCGGAC 
ACCTCTAGTT CAAGCCGAAT TGAACGAAAA ATTGCTACTA TTTCCTTAGA CCGAGATGGA 
CCAGTTAGAG ACTTGGAAAG AGGTTTACAG GGACGTAAAT CAGGTGCCAG CAGCGTGAGC 
AGCGAGGTAT CCTCTTCCTC CGGAACCAGC AAGTGGAAAT CCACCTTCTC CCCCATTTCT 
GATCCCAAGC CAACTCCTCC AGACATCCGC CAAGGTGGCT CACCATTTAG CGTTGGAGCT 
TCCCGAGGAG GCACAGACTC AGACTCGGAC CACAAACCCC AGAGGAGAGG GGAGCGTGAG 
CGTGAGCCTT ATGGAAATTC AAATCTCTTC CTCAGTCAGG ATGGCAGTGG TCGCTCGGCG 
GTAAACACGT CAGAGCGTTC GCTGCAGAAA CAGAAAGTGC GGGAGTGGGA CCTGAAATCT 
GTAAGTGGAT TGGCCAGTCA AAACCTATTT ATATCTGCAG CAGCCAGTGG AGGCATCCTG 
AGTGGGAAAG TGGGCGGGGC TGCCACTGTG TCATCCGCCT CTGCGGTAGG GCAATATTTC 
CCACTGGGCG GAGCTTCTGT TCTACAACCC TTATTTGGAA CTCAGGCAGC GGGACCAGCC 
ACCAGCGGAG CCCCCCGATT GGTCAACGGA CATTCGGCGC TTGGCAGTTT CTCCAGTGCC 
GGACTGGCAG GGGGCGCTGC AGGAGGGATT TTTCACCACA TGGTGTCTTC AAGCTCCTCA 
CAGCAGTCTG GGCCCAATCT CCCAAGCTCC CATAATTCCC AACAGAGGCA TCTGGACACG 
AACCCCAAAG CAGGGGCCTC GTTCTTGGCC TCTGGCCCTA ACCGCACTCA AACGGTCCTA 
CACGCACCCT CTCCACCCAC GCTCGCTCTT CCCCCGCCCC CACCCCTCCT GAACACTGTC 
CCGTCCCAAC CGTCCTCTCC GCGCTCATCC AGACCGCAGT CGTCCATCCC CGTGGCCACC 
TCGTCCCAAT CCCTGGCTGC CGCGTCCTCT TCCTGCACCT CATCCGCTTC CTCAAGGCCC 
TTCACTGTCC ACTATCCTCC GCATCTGCAG CCTCCGGGCA GCGCTGGTTC AGGTGGGACG 
GGGGCCTCCT GGAGGACTCT GGCATCCTAT ACGGCCTCGC AGATTCCTGG GAATCGGCCC 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_005170534.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170591.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26968
Clone ID Related Accession (Same CDS sequence)	XM_005170534.4 , XM_005170534.3
Accession Version	XM_005170534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170534.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGGATTTTTC ACCACATGGT GTCTTCAAGC 
TCCTCACAGC AGTCTGGGCC CAATCTCCCA AGCTCCCATA ATTCCCAACA GAGGCATCTG 
GACACGAACC CCAAAGCAGG GGCCTCGTTC TTGGCCTCTG GCCCTAACCG CACTCAAACG 
GTCCTACACG CACCCTCTCC ACCCACGCTC GCTCTTCCCC CGCCCCCACC CCTCCTGAAC 
ACTGTCCCGT CCCAACCGTC CTCTCCGCGC TCATCCAGAC CGCAGTCGTC CATCCCCGTG 
GCCACCTCGT CCCAATCCCT GGCTGCCGCG TCCTCTTCCT GCACCTCATC CGCTTCCTCA 
AGGCCCTTCA CTGTCCACTA TCCTCCGCAT CTGCAGCCTC CGGGCAGCGC TGGTTCAGGT 
GGGACGGGGG CCTCCTGGAG GACTCTGGCA TCCTATACGG CCTCGCAGAT TCCTGGGAAT 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170591.1
CDS	257..4828
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR

Target ORF information:

RefSeq Version	XM_005170534.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170534.4

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGGATTTTTC ACCACATGGT GTCTTCAAGC 
TCCTCACAGC AGTCTGGGCC CAATCTCCCA AGCTCCCATA ATTCCCAACA GAGGCATCTG 
GACACGAACC CCAAAGCAGG GGCCTCGTTC TTGGCCTCTG GCCCTAACCG CACTCAAACG 
GTCCTACACG CACCCTCTCC ACCCACGCTC GCTCTTCCCC CGCCCCCACC CCTCCTGAAC 
ACTGTCCCGT CCCAACCGTC CTCTCCGCGC TCATCCAGAC CGCAGTCGTC CATCCCCGTG 
GCCACCTCGT CCCAATCCCT GGCTGCCGCG TCCTCTTCCT GCACCTCATC CGCTTCCTCA 
AGGCCCTTCA CTGTCCACTA TCCTCCGCAT CTGCAGCCTC CGGGCAGCGC TGGTTCAGGT 
GGGACGGGGG CCTCCTGGAG GACTCTGGCA TCCTATACGG CCTCGCAGAT TCCTGGGAAT 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_005170534.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170591.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26968
Clone ID Related Accession (Same CDS sequence)	XM_005170534.4 , XM_005170534.3
Accession Version	XM_005170534.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGGATTTTTC ACCACATGGT GTCTTCAAGC 
TCCTCACAGC AGTCTGGGCC CAATCTCCCA AGCTCCCATA ATTCCCAACA GAGGCATCTG 
GACACGAACC CCAAAGCAGG GGCCTCGTTC TTGGCCTCTG GCCCTAACCG CACTCAAACG 
GTCCTACACG CACCCTCTCC ACCCACGCTC GCTCTTCCCC CGCCCCCACC CCTCCTGAAC 
ACTGTCCCGT CCCAACCGTC CTCTCCGCGC TCATCCAGAC CGCAGTCGTC CATCCCCGTG 
GCCACCTCGT CCCAATCCCT GGCTGCCGCG TCCTCTTCCT GCACCTCATC CGCTTCCTCA 
AGGCCCTTCA CTGTCCACTA TCCTCCGCAT CTGCAGCCTC CGGGCAGCGC TGGTTCAGGT 
GGGACGGGGG CCTCCTGGAG GACTCTGGCA TCCTATACGG CCTCGCAGAT TCCTGGGAAT 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170591.1
CDS	254..4825
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR

Target ORF information:

RefSeq Version	XM_005170534.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170534.3

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGGATTTTTC ACCACATGGT GTCTTCAAGC 
TCCTCACAGC AGTCTGGGCC CAATCTCCCA AGCTCCCATA ATTCCCAACA GAGGCATCTG 
GACACGAACC CCAAAGCAGG GGCCTCGTTC TTGGCCTCTG GCCCTAACCG CACTCAAACG 
GTCCTACACG CACCCTCTCC ACCCACGCTC GCTCTTCCCC CGCCCCCACC CCTCCTGAAC 
ACTGTCCCGT CCCAACCGTC CTCTCCGCGC TCATCCAGAC CGCAGTCGTC CATCCCCGTG 
GCCACCTCGT CCCAATCCCT GGCTGCCGCG TCCTCTTCCT GCACCTCATC CGCTTCCTCA 
AGGCCCTTCA CTGTCCACTA TCCTCCGCAT CTGCAGCCTC CGGGCAGCGC TGGTTCAGGT 
GGGACGGGGG CCTCCTGGAG GACTCTGGCA TCCTATACGG CCTCGCAGAT TCCTGGGAAT 
CGGCCCAGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_009296351.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294626.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

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CloneID	ODa43204
Clone ID Related Accession (Same CDS sequence)	XM_009296351.2 , XM_009296351.3
Accession Version	XM_009296351.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGGC GCAGCATGAT TCGGGTGGTG AAGAGGAGCC AGTTGTCCCC 
GTGAAGCCGT CACCCAAACC CCGCCGGGCC AAAATTCTGA CCAGGGGCCG CAAGCTTGGC 
AACAGGAAGC GCGGGCGACC CAAGAAGGCA CCAGCTGCAA CTGCAGCCGA GCGCAAGAAC 
AAGAAGAGCC AGAGCGCCCT CGACCTGCTG CACGCCAAGA CCCTCTCAGC GGCCCCTGCT 
CAGGAACCAT ACCGGTCACC TCAGAGTCCG TTCTATCAGC TACCTCCCAA AGTGCAGCAT 
TACACATCCA GCCAGCTCCT CATGGCTCCC ACTCCACCTG GCCTGCAGAC GCTACTGGAT 
AACATCAAAG TCCAGTACCT GCACTTCATG GCCTACATGA AGACGCCACA GTACCGCACC 
AACCTGCAGC AATCGCTTGA GCAGGAGAAG CTGAGACACA CAGAACTGTC AAGTCAAGCC 
CAGCAGCTGG TCAACGCTTG TCATGCTCAC AAGGAGAGGA TTCATGACCT TTTCCAGTCC 
AAACTAGAAG AGCTGGGTGT GAAGGCGGTG ACGGTGGAGG ATCTGGTTCA GGCTCAGAAG 
GAGATTACAG CCCACAACAT GCAGCTGCGC GAACAGACCA AACAGCTGGA GCATGACATG 
GCCGAGCTCC GAGATCAGAG CCAGCTGCTG CTGAAGGCTC GCTGTGAGGA GCTCAAGCTG 
GACACTCTGT GTTGGGACAC TCTGAAGAAA GAGATAGAAG CTCTGCGGAG ACAGATCTCT 
GAGAAACAGC GCCACTGTCT GGAGCTCCAG ATCAGTATAG TTGAACTCGA GAAGAGCCAG 
CGACAGCAGG AGCTCCTTCA GCTCAAGTCA TCCTACAGCC CTTGTGAAGT GTCCCCCTAC 
AGAAAAGCCC TCCCTGGCCC TGATCCTCGG CCCACACTGG ACCCGGATAC CCCAAAACTC 
ACCCCGCAAT CCTCTATGGG GCTGAACGGC CTCAGTCCTG AACTCTCTAT CAACGGCACT 
GCCTCTCCGG GTTATGAAAG ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC 
CACTACCTGC CCATTTCTCC AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC 
AGGCAGCTTG GGCAACCCCT GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC 
CGCAGGCACC TCAACCAGGA TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT 
CGGGGCGACA CTGGTGCCAT TTCATCTCCA ACACTGGGCT TTAAACAGGC TTCACCATGT 
TCCTCGGAAG TTCAAGCGGT CACCACAGCC AAGAGCACAG ATAGGGCCGA TAAAGAAAAG 
AGCCCGACTG CACCCGGCGA CACTATAACC AGTCTACCAA TCAGCATCCC TCTCAGCACT 
GTGCATCCCA GCAAACTTCC TGTCAGCATC CCTCTGGCCA GTGTGGTGCT GCCGAACCGC 
GTCGAAAGAC TGAGGAGCAC TCTTAGTCCG GTATTGAATC CAGCAGGACA GAGCAATGGC 
AAGCCACCTC TCACGCCTAC CTCAGGGTAC TCCTCCAGCT CAGGATTGGT TAATGGGTCT 
CACTCCACCA TGCTGACCGA GGACCAAGAC TGTGATGTTG CCTCCCCTCC TCCTCACAGC 
ACTCCCGTCA TGGGTCTTCA GCCCCGCGGC TCTGGCCTCA GTCCTCCACT GGGCACCGGA 
GGCGTTCTCC AGTACGCCGA CGGTCCACCA AGGCTTCCCC CCGAGGATGG CCATGAGCGC 
CAAGGCCCCG AATCCGACAC AGAGCCCTTA GATAGTGAGG CTCGCCGGCG CATTTTTTTC 
TCTTCATCCT CCTCTTCTTC CTCATCCTCA TCCTCCACTG GAGGCTCGAG GCTGCACCAT 
GGGTCTGTAA AGCAGGGTCA CCATCATCAC AGTAAGCACC ATCACCACCA CCACCACCAT 
CATCACCATC ACTCTCCAAG CTCCCACAGC CAAGAGGGCC GAAAACGAGG AAGACGAAAG 
CGCAGCTCAT CTGGAGCCGT GCCCATGAGT GGATCCCCCA AAAGAAGGGT CTTTCCTGGA 
CTGGGCTGCT CAAGTCATTC CTCTGGATCA CCGCTTAATA TCAATTCAAT GGTAAACAAC 
ATAAACCAGC CTTTGGAGAT CTCCGCCATT TCTTCCCCAG AGCAGTCAGG CAGAAGCCCA 
TGTGAGCAAG ATCTGGATCA GCCACCTGTT TTGAAACGAG AGCGTCCTCT TGAGCTCAAC 
AACACCGGCC GTTATTCCTC TGCCCCAAGT TCAGATGAAG AAGGCAATGG TTACGCCCCG 
GACACCTCTA GTTCAAGCCG AATTGAACGA AAAATTGCTA CTATTTCCTT AGACCGAGAT 
GGACCAGTTA GAGACTTGGA AAGAGGTTTA CAGGGACGTA AATCAGGTGC CAGCAGCGTG 
AGCAGCGAGG TATCCTCTTC CTCCGGAACC AGCAAGTGGA AATCCACCTT CTCCCCCATT 
TCTGATCCCA AGCCAACTCC TCCAGACATC CGCCAAGGTG GCTCACCATT TAGCGTTGGA 
GCTTCCCGAG GAGGCACAGA CTCAGACTCG GACCACAAAC CCCAGAGGAG AGGGGAGCGT 
GAGCGTGAGC CTTATGGAAA TTCAAATCTC TTCCTCAGTC AGGATGGCAG TGGTCGCTCG 
GCGGTAAACA CGTCAGAGCG TTCGCTGCAG AAACAGAAAG TGCGGGAGTG GGACCTGAAA 
TCTGTAAGTG GATTGGCCAG TCAAAACCTA TTTATATCTG CAGCAGCCAG TGGAGGCATC 
CTGAGTGGGA AAGTGGGCGG GGCTGCCACT GTGTCATCCG CCTCTGCGGT AGGGCAATAT 
TTCCCACTGG GCGGAGCTTC TGTTCTACAA CCCTTATTTG GAACTCAGGC AGCGGGACCA 
GCCACCAGCG GAGCCCCCCG ATTGGTCAAC GGACATTCGG CGCTTGGCAG TTTCTCCAGT 
GCCGGACTGG CAGGGGGCGC TGCAGGAGGG ATTTTTCACC ACATGGTGTC TTCAAGCTCC 
TCACAGCAGT CTGGGCCCAA TCTCCCAAGC TCCCATAATT CCCAACAGAG GCATCTGGAC 
ACGAACCCCA AAGCAGGGGC CTCGTTCTTG GCCTCTGGCC CTAACCGCAC TCAAACGGTC 
CTACACGCAC CCTCTCCACC CACGCTCGCT CTTCCCCCGC CCCCACCCCT CCTGAACACT 
GTCCCGTCCC AACCGTCCTC TCCGCGCTCA TCCAGACCGC AGTCGTCCAT CCCCGTGGCC 
ACCTCGTCCC AATCCCTGGC TGCCGCGTCC TCTTCCTGCA CCTCATCCGC TTCCTCAAGG 
CCCTTCACTG TCCACTATCC TCCGCATCTG CAGCCTCCGG GCAGCGCTGG TTCAGGTGGG 
ACGGGGGCCT CCTGGAGGAC TCTGGCATCC TATACGGCCT CGCAGATTCC TGGGAATCGG 
CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294626.1
CDS	254..4822
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR

Target ORF information:

RefSeq Version	XM_009296351.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296351.2

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGGC GCAGCATGAT TCGGGTGGTG AAGAGGAGCC AGTTGTCCCC 
GTGAAGCCGT CACCCAAACC CCGCCGGGCC AAAATTCTGA CCAGGGGCCG CAAGCTTGGC 
AACAGGAAGC GCGGGCGACC CAAGAAGGCA CCAGCTGCAA CTGCAGCCGA GCGCAAGAAC 
AAGAAGAGCC AGAGCGCCCT CGACCTGCTG CACGCCAAGA CCCTCTCAGC GGCCCCTGCT 
CAGGAACCAT ACCGGTCACC TCAGAGTCCG TTCTATCAGC TACCTCCCAA AGTGCAGCAT 
TACACATCCA GCCAGCTCCT CATGGCTCCC ACTCCACCTG GCCTGCAGAC GCTACTGGAT 
AACATCAAAG TCCAGTACCT GCACTTCATG GCCTACATGA AGACGCCACA GTACCGCACC 
AACCTGCAGC AATCGCTTGA GCAGGAGAAG CTGAGACACA CAGAACTGTC AAGTCAAGCC 
CAGCAGCTGG TCAACGCTTG TCATGCTCAC AAGGAGAGGA TTCATGACCT TTTCCAGTCC 
AAACTAGAAG AGCTGGGTGT GAAGGCGGTG ACGGTGGAGG ATCTGGTTCA GGCTCAGAAG 
GAGATTACAG CCCACAACAT GCAGCTGCGC GAACAGACCA AACAGCTGGA GCATGACATG 
GCCGAGCTCC GAGATCAGAG CCAGCTGCTG CTGAAGGCTC GCTGTGAGGA GCTCAAGCTG 
GACACTCTGT GTTGGGACAC TCTGAAGAAA GAGATAGAAG CTCTGCGGAG ACAGATCTCT 
GAGAAACAGC GCCACTGTCT GGAGCTCCAG ATCAGTATAG TTGAACTCGA GAAGAGCCAG 
CGACAGCAGG AGCTCCTTCA GCTCAAGTCA TCCTACAGCC CTTGTGAAGT GTCCCCCTAC 
AGAAAAGCCC TCCCTGGCCC TGATCCTCGG CCCACACTGG ACCCGGATAC CCCAAAACTC 
ACCCCGCAAT CCTCTATGGG GCTGAACGGC CTCAGTCCTG AACTCTCTAT CAACGGCACT 
GCCTCTCCGG GTTATGAAAG ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC 
CACTACCTGC CCATTTCTCC AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC 
AGGCAGCTTG GGCAACCCCT GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC 
CGCAGGCACC TCAACCAGGA TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT 
CGGGGCGACA CTGGTGCCAT TTCATCTCCA ACACTGGGCT TTAAACAGGC TTCACCATGT 
TCCTCGGAAG TTCAAGCGGT CACCACAGCC AAGAGCACAG ATAGGGCCGA TAAAGAAAAG 
AGCCCGACTG CACCCGGCGA CACTATAACC AGTCTACCAA TCAGCATCCC TCTCAGCACT 
GTGCATCCCA GCAAACTTCC TGTCAGCATC CCTCTGGCCA GTGTGGTGCT GCCGAACCGC 
GTCGAAAGAC TGAGGAGCAC TCTTAGTCCG GTATTGAATC CAGCAGGACA GAGCAATGGC 
AAGCCACCTC TCACGCCTAC CTCAGGGTAC TCCTCCAGCT CAGGATTGGT TAATGGGTCT 
CACTCCACCA TGCTGACCGA GGACCAAGAC TGTGATGTTG CCTCCCCTCC TCCTCACAGC 
ACTCCCGTCA TGGGTCTTCA GCCCCGCGGC TCTGGCCTCA GTCCTCCACT GGGCACCGGA 
GGCGTTCTCC AGTACGCCGA CGGTCCACCA AGGCTTCCCC CCGAGGATGG CCATGAGCGC 
CAAGGCCCCG AATCCGACAC AGAGCCCTTA GATAGTGAGG CTCGCCGGCG CATTTTTTTC 
TCTTCATCCT CCTCTTCTTC CTCATCCTCA TCCTCCACTG GAGGCTCGAG GCTGCACCAT 
GGGTCTGTAA AGCAGGGTCA CCATCATCAC AGTAAGCACC ATCACCACCA CCACCACCAT 
CATCACCATC ACTCTCCAAG CTCCCACAGC CAAGAGGGCC GAAAACGAGG AAGACGAAAG 
CGCAGCTCAT CTGGAGCCGT GCCCATGAGT GGATCCCCCA AAAGAAGGGT CTTTCCTGGA 
CTGGGCTGCT CAAGTCATTC CTCTGGATCA CCGCTTAATA TCAATTCAAT GGTAAACAAC 
ATAAACCAGC CTTTGGAGAT CTCCGCCATT TCTTCCCCAG AGCAGTCAGG CAGAAGCCCA 
TGTGAGCAAG ATCTGGATCA GCCACCTGTT TTGAAACGAG AGCGTCCTCT TGAGCTCAAC 
AACACCGGCC GTTATTCCTC TGCCCCAAGT TCAGATGAAG AAGGCAATGG TTACGCCCCG 
GACACCTCTA GTTCAAGCCG AATTGAACGA AAAATTGCTA CTATTTCCTT AGACCGAGAT 
GGACCAGTTA GAGACTTGGA AAGAGGTTTA CAGGGACGTA AATCAGGTGC CAGCAGCGTG 
AGCAGCGAGG TATCCTCTTC CTCCGGAACC AGCAAGTGGA AATCCACCTT CTCCCCCATT 
TCTGATCCCA AGCCAACTCC TCCAGACATC CGCCAAGGTG GCTCACCATT TAGCGTTGGA 
GCTTCCCGAG GAGGCACAGA CTCAGACTCG GACCACAAAC CCCAGAGGAG AGGGGAGCGT 
GAGCGTGAGC CTTATGGAAA TTCAAATCTC TTCCTCAGTC AGGATGGCAG TGGTCGCTCG 
GCGGTAAACA CGTCAGAGCG TTCGCTGCAG AAACAGAAAG TGCGGGAGTG GGACCTGAAA 
TCTGTAAGTG GATTGGCCAG TCAAAACCTA TTTATATCTG CAGCAGCCAG TGGAGGCATC 
CTGAGTGGGA AAGTGGGCGG GGCTGCCACT GTGTCATCCG CCTCTGCGGT AGGGCAATAT 
TTCCCACTGG GCGGAGCTTC TGTTCTACAA CCCTTATTTG GAACTCAGGC AGCGGGACCA 
GCCACCAGCG GAGCCCCCCG ATTGGTCAAC GGACATTCGG CGCTTGGCAG TTTCTCCAGT 
GCCGGACTGG CAGGGGGCGC TGCAGGAGGG ATTTTTCACC ACATGGTGTC TTCAAGCTCC 
TCACAGCAGT CTGGGCCCAA TCTCCCAAGC TCCCATAATT CCCAACAGAG GCATCTGGAC 
ACGAACCCCA AAGCAGGGGC CTCGTTCTTG GCCTCTGGCC CTAACCGCAC TCAAACGGTC 
CTACACGCAC CCTCTCCACC CACGCTCGCT CTTCCCCCGC CCCCACCCCT CCTGAACACT 
GTCCCGTCCC AACCGTCCTC TCCGCGCTCA TCCAGACCGC AGTCGTCCAT CCCCGTGGCC 
ACCTCGTCCC AATCCCTGGC TGCCGCGTCC TCTTCCTGCA CCTCATCCGC TTCCTCAAGG 
CCCTTCACTG TCCACTATCC TCCGCATCTG CAGCCTCCGG GCAGCGCTGG TTCAGGTGGG 
ACGGGGGCCT CCTGGAGGAC TCTGGCATCC TATACGGCCT CGCAGATTCC TGGGAATCGG 
CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_009296351.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294626.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

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CloneID	ODa43204
Clone ID Related Accession (Same CDS sequence)	XM_009296351.2 , XM_009296351.3
Accession Version	XM_009296351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGGC GCAGCATGAT TCGGGTGGTG AAGAGGAGCC AGTTGTCCCC 
GTGAAGCCGT CACCCAAACC CCGCCGGGCC AAAATTCTGA CCAGGGGCCG CAAGCTTGGC 
AACAGGAAGC GCGGGCGACC CAAGAAGGCA CCAGCTGCAA CTGCAGCCGA GCGCAAGAAC 
AAGAAGAGCC AGAGCGCCCT CGACCTGCTG CACGCCAAGA CCCTCTCAGC GGCCCCTGCT 
CAGGAACCAT ACCGGTCACC TCAGAGTCCG TTCTATCAGC TACCTCCCAA AGTGCAGCAT 
TACACATCCA GCCAGCTCCT CATGGCTCCC ACTCCACCTG GCCTGCAGAC GCTACTGGAT 
AACATCAAAG TCCAGTACCT GCACTTCATG GCCTACATGA AGACGCCACA GTACCGCACC 
AACCTGCAGC AATCGCTTGA GCAGGAGAAG CTGAGACACA CAGAACTGTC AAGTCAAGCC 
CAGCAGCTGG TCAACGCTTG TCATGCTCAC AAGGAGAGGA TTCATGACCT TTTCCAGTCC 
AAACTAGAAG AGCTGGGTGT GAAGGCGGTG ACGGTGGAGG ATCTGGTTCA GGCTCAGAAG 
GAGATTACAG CCCACAACAT GCAGCTGCGC GAACAGACCA AACAGCTGGA GCATGACATG 
GCCGAGCTCC GAGATCAGAG CCAGCTGCTG CTGAAGGCTC GCTGTGAGGA GCTCAAGCTG 
GACACTCTGT GTTGGGACAC TCTGAAGAAA GAGATAGAAG CTCTGCGGAG ACAGATCTCT 
GAGAAACAGC GCCACTGTCT GGAGCTCCAG ATCAGTATAG TTGAACTCGA GAAGAGCCAG 
CGACAGCAGG AGCTCCTTCA GCTCAAGTCA TCCTACAGCC CTTGTGAAGT GTCCCCCTAC 
AGAAAAGCCC TCCCTGGCCC TGATCCTCGG CCCACACTGG ACCCGGATAC CCCAAAACTC 
ACCCCGCAAT CCTCTATGGG GCTGAACGGC CTCAGTCCTG AACTCTCTAT CAACGGCACT 
GCCTCTCCGG GTTATGAAAG ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC 
CACTACCTGC CCATTTCTCC AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC 
AGGCAGCTTG GGCAACCCCT GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC 
CGCAGGCACC TCAACCAGGA TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT 
CGGGGCGACA CTGGTGCCAT TTCATCTCCA ACACTGGGCT TTAAACAGGC TTCACCATGT 
TCCTCGGAAG TTCAAGCGGT CACCACAGCC AAGAGCACAG ATAGGGCCGA TAAAGAAAAG 
AGCCCGACTG CACCCGGCGA CACTATAACC AGTCTACCAA TCAGCATCCC TCTCAGCACT 
GTGCATCCCA GCAAACTTCC TGTCAGCATC CCTCTGGCCA GTGTGGTGCT GCCGAACCGC 
GTCGAAAGAC TGAGGAGCAC TCTTAGTCCG GTATTGAATC CAGCAGGACA GAGCAATGGC 
AAGCCACCTC TCACGCCTAC CTCAGGGTAC TCCTCCAGCT CAGGATTGGT TAATGGGTCT 
CACTCCACCA TGCTGACCGA GGACCAAGAC TGTGATGTTG CCTCCCCTCC TCCTCACAGC 
ACTCCCGTCA TGGGTCTTCA GCCCCGCGGC TCTGGCCTCA GTCCTCCACT GGGCACCGGA 
GGCGTTCTCC AGTACGCCGA CGGTCCACCA AGGCTTCCCC CCGAGGATGG CCATGAGCGC 
CAAGGCCCCG AATCCGACAC AGAGCCCTTA GATAGTGAGG CTCGCCGGCG CATTTTTTTC 
TCTTCATCCT CCTCTTCTTC CTCATCCTCA TCCTCCACTG GAGGCTCGAG GCTGCACCAT 
GGGTCTGTAA AGCAGGGTCA CCATCATCAC AGTAAGCACC ATCACCACCA CCACCACCAT 
CATCACCATC ACTCTCCAAG CTCCCACAGC CAAGAGGGCC GAAAACGAGG AAGACGAAAG 
CGCAGCTCAT CTGGAGCCGT GCCCATGAGT GGATCCCCCA AAAGAAGGGT CTTTCCTGGA 
CTGGGCTGCT CAAGTCATTC CTCTGGATCA CCGCTTAATA TCAATTCAAT GGTAAACAAC 
ATAAACCAGC CTTTGGAGAT CTCCGCCATT TCTTCCCCAG AGCAGTCAGG CAGAAGCCCA 
TGTGAGCAAG ATCTGGATCA GCCACCTGTT TTGAAACGAG AGCGTCCTCT TGAGCTCAAC 
AACACCGGCC GTTATTCCTC TGCCCCAAGT TCAGATGAAG AAGGCAATGG TTACGCCCCG 
GACACCTCTA GTTCAAGCCG AATTGAACGA AAAATTGCTA CTATTTCCTT AGACCGAGAT 
GGACCAGTTA GAGACTTGGA AAGAGGTTTA CAGGGACGTA AATCAGGTGC CAGCAGCGTG 
AGCAGCGAGG TATCCTCTTC CTCCGGAACC AGCAAGTGGA AATCCACCTT CTCCCCCATT 
TCTGATCCCA AGCCAACTCC TCCAGACATC CGCCAAGGTG GCTCACCATT TAGCGTTGGA 
GCTTCCCGAG GAGGCACAGA CTCAGACTCG GACCACAAAC CCCAGAGGAG AGGGGAGCGT 
GAGCGTGAGC CTTATGGAAA TTCAAATCTC TTCCTCAGTC AGGATGGCAG TGGTCGCTCG 
GCGGTAAACA CGTCAGAGCG TTCGCTGCAG AAACAGAAAG TGCGGGAGTG GGACCTGAAA 
TCTGTAAGTG GATTGGCCAG TCAAAACCTA TTTATATCTG CAGCAGCCAG TGGAGGCATC 
CTGAGTGGGA AAGTGGGCGG GGCTGCCACT GTGTCATCCG CCTCTGCGGT AGGGCAATAT 
TTCCCACTGG GCGGAGCTTC TGTTCTACAA CCCTTATTTG GAACTCAGGC AGCGGGACCA 
GCCACCAGCG GAGCCCCCCG ATTGGTCAAC GGACATTCGG CGCTTGGCAG TTTCTCCAGT 
GCCGGACTGG CAGGGGGCGC TGCAGGAGGG ATTTTTCACC ACATGGTGTC TTCAAGCTCC 
TCACAGCAGT CTGGGCCCAA TCTCCCAAGC TCCCATAATT CCCAACAGAG GCATCTGGAC 
ACGAACCCCA AAGCAGGGGC CTCGTTCTTG GCCTCTGGCC CTAACCGCAC TCAAACGGTC 
CTACACGCAC CCTCTCCACC CACGCTCGCT CTTCCCCCGC CCCCACCCCT CCTGAACACT 
GTCCCGTCCC AACCGTCCTC TCCGCGCTCA TCCAGACCGC AGTCGTCCAT CCCCGTGGCC 
ACCTCGTCCC AATCCCTGGC TGCCGCGTCC TCTTCCTGCA CCTCATCCGC TTCCTCAAGG 
CCCTTCACTG TCCACTATCC TCCGCATCTG CAGCCTCCGG GCAGCGCTGG TTCAGGTGGG 
ACGGGGGCCT CCTGGAGGAC TCTGGCATCC TATACGGCCT CGCAGATTCC TGGGAATCGG 
CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294626.1
CDS	257..4825
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGIFHHMVSSSSSQQSGPNLPSSHNSQQRHLDTNPKAGASFLASGPNRTQTVLHAPSPPTLALPPPPPLLNTVPSQPSSPRSSRPQSSIPVATSSQSLAAASSSCTSSASSRPFTVHYPPHLQPPGSAGSGGTGASWRTLASYTASQIPGNRPR

Target ORF information:

RefSeq Version	XM_009296351.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296351.3

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGGC GCAGCATGAT TCGGGTGGTG AAGAGGAGCC AGTTGTCCCC 
GTGAAGCCGT CACCCAAACC CCGCCGGGCC AAAATTCTGA CCAGGGGCCG CAAGCTTGGC 
AACAGGAAGC GCGGGCGACC CAAGAAGGCA CCAGCTGCAA CTGCAGCCGA GCGCAAGAAC 
AAGAAGAGCC AGAGCGCCCT CGACCTGCTG CACGCCAAGA CCCTCTCAGC GGCCCCTGCT 
CAGGAACCAT ACCGGTCACC TCAGAGTCCG TTCTATCAGC TACCTCCCAA AGTGCAGCAT 
TACACATCCA GCCAGCTCCT CATGGCTCCC ACTCCACCTG GCCTGCAGAC GCTACTGGAT 
AACATCAAAG TCCAGTACCT GCACTTCATG GCCTACATGA AGACGCCACA GTACCGCACC 
AACCTGCAGC AATCGCTTGA GCAGGAGAAG CTGAGACACA CAGAACTGTC AAGTCAAGCC 
CAGCAGCTGG TCAACGCTTG TCATGCTCAC AAGGAGAGGA TTCATGACCT TTTCCAGTCC 
AAACTAGAAG AGCTGGGTGT GAAGGCGGTG ACGGTGGAGG ATCTGGTTCA GGCTCAGAAG 
GAGATTACAG CCCACAACAT GCAGCTGCGC GAACAGACCA AACAGCTGGA GCATGACATG 
GCCGAGCTCC GAGATCAGAG CCAGCTGCTG CTGAAGGCTC GCTGTGAGGA GCTCAAGCTG 
GACACTCTGT GTTGGGACAC TCTGAAGAAA GAGATAGAAG CTCTGCGGAG ACAGATCTCT 
GAGAAACAGC GCCACTGTCT GGAGCTCCAG ATCAGTATAG TTGAACTCGA GAAGAGCCAG 
CGACAGCAGG AGCTCCTTCA GCTCAAGTCA TCCTACAGCC CTTGTGAAGT GTCCCCCTAC 
AGAAAAGCCC TCCCTGGCCC TGATCCTCGG CCCACACTGG ACCCGGATAC CCCAAAACTC 
ACCCCGCAAT CCTCTATGGG GCTGAACGGC CTCAGTCCTG AACTCTCTAT CAACGGCACT 
GCCTCTCCGG GTTATGAAAG ATGCACCGGA AGTGCAATGG GCAAAAACGA ACTCCTCACC 
CACTACCTGC CCATTTCTCC AGACCACGAA ATTGTGCCGC CAACCCCGGA CTCCAGAACC 
AGGCAGCTTG GGCAACCCCT GCCCGACTAC ACCCGCTTTT CTCCAGCAAA GATCGCCCTC 
CGCAGGCACC TCAACCAGGA TTCAGCCGCA AACCAGTTCC GGGCACTCGG AAACATCCAT 
CGGGGCGACA CTGGTGCCAT TTCATCTCCA ACACTGGGCT TTAAACAGGC TTCACCATGT 
TCCTCGGAAG TTCAAGCGGT CACCACAGCC AAGAGCACAG ATAGGGCCGA TAAAGAAAAG 
AGCCCGACTG CACCCGGCGA CACTATAACC AGTCTACCAA TCAGCATCCC TCTCAGCACT 
GTGCATCCCA GCAAACTTCC TGTCAGCATC CCTCTGGCCA GTGTGGTGCT GCCGAACCGC 
GTCGAAAGAC TGAGGAGCAC TCTTAGTCCG GTATTGAATC CAGCAGGACA GAGCAATGGC 
AAGCCACCTC TCACGCCTAC CTCAGGGTAC TCCTCCAGCT CAGGATTGGT TAATGGGTCT 
CACTCCACCA TGCTGACCGA GGACCAAGAC TGTGATGTTG CCTCCCCTCC TCCTCACAGC 
ACTCCCGTCA TGGGTCTTCA GCCCCGCGGC TCTGGCCTCA GTCCTCCACT GGGCACCGGA 
GGCGTTCTCC AGTACGCCGA CGGTCCACCA AGGCTTCCCC CCGAGGATGG CCATGAGCGC 
CAAGGCCCCG AATCCGACAC AGAGCCCTTA GATAGTGAGG CTCGCCGGCG CATTTTTTTC 
TCTTCATCCT CCTCTTCTTC CTCATCCTCA TCCTCCACTG GAGGCTCGAG GCTGCACCAT 
GGGTCTGTAA AGCAGGGTCA CCATCATCAC AGTAAGCACC ATCACCACCA CCACCACCAT 
CATCACCATC ACTCTCCAAG CTCCCACAGC CAAGAGGGCC GAAAACGAGG AAGACGAAAG 
CGCAGCTCAT CTGGAGCCGT GCCCATGAGT GGATCCCCCA AAAGAAGGGT CTTTCCTGGA 
CTGGGCTGCT CAAGTCATTC CTCTGGATCA CCGCTTAATA TCAATTCAAT GGTAAACAAC 
ATAAACCAGC CTTTGGAGAT CTCCGCCATT TCTTCCCCAG AGCAGTCAGG CAGAAGCCCA 
TGTGAGCAAG ATCTGGATCA GCCACCTGTT TTGAAACGAG AGCGTCCTCT TGAGCTCAAC 
AACACCGGCC GTTATTCCTC TGCCCCAAGT TCAGATGAAG AAGGCAATGG TTACGCCCCG 
GACACCTCTA GTTCAAGCCG AATTGAACGA AAAATTGCTA CTATTTCCTT AGACCGAGAT 
GGACCAGTTA GAGACTTGGA AAGAGGTTTA CAGGGACGTA AATCAGGTGC CAGCAGCGTG 
AGCAGCGAGG TATCCTCTTC CTCCGGAACC AGCAAGTGGA AATCCACCTT CTCCCCCATT 
TCTGATCCCA AGCCAACTCC TCCAGACATC CGCCAAGGTG GCTCACCATT TAGCGTTGGA 
GCTTCCCGAG GAGGCACAGA CTCAGACTCG GACCACAAAC CCCAGAGGAG AGGGGAGCGT 
GAGCGTGAGC CTTATGGAAA TTCAAATCTC TTCCTCAGTC AGGATGGCAG TGGTCGCTCG 
GCGGTAAACA CGTCAGAGCG TTCGCTGCAG AAACAGAAAG TGCGGGAGTG GGACCTGAAA 
TCTGTAAGTG GATTGGCCAG TCAAAACCTA TTTATATCTG CAGCAGCCAG TGGAGGCATC 
CTGAGTGGGA AAGTGGGCGG GGCTGCCACT GTGTCATCCG CCTCTGCGGT AGGGCAATAT 
TTCCCACTGG GCGGAGCTTC TGTTCTACAA CCCTTATTTG GAACTCAGGC AGCGGGACCA 
GCCACCAGCG GAGCCCCCCG ATTGGTCAAC GGACATTCGG CGCTTGGCAG TTTCTCCAGT 
GCCGGACTGG CAGGGGGCGC TGCAGGAGGG ATTTTTCACC ACATGGTGTC TTCAAGCTCC 
TCACAGCAGT CTGGGCCCAA TCTCCCAAGC TCCCATAATT CCCAACAGAG GCATCTGGAC 
ACGAACCCCA AAGCAGGGGC CTCGTTCTTG GCCTCTGGCC CTAACCGCAC TCAAACGGTC 
CTACACGCAC CCTCTCCACC CACGCTCGCT CTTCCCCCGC CCCCACCCCT CCTGAACACT 
GTCCCGTCCC AACCGTCCTC TCCGCGCTCA TCCAGACCGC AGTCGTCCAT CCCCGTGGCC 
ACCTCGTCCC AATCCCTGGC TGCCGCGTCC TCTTCCTGCA CCTCATCCGC TTCCTCAAGG 
CCCTTCACTG TCCACTATCC TCCGCATCTG CAGCCTCCGG GCAGCGCTGG TTCAGGTGGG 
ACGGGGGCCT CCTGGAGGAC TCTGGCATCC TATACGGCCT CGCAGATTCC TGGGAATCGG 
CCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_009296352.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294627.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

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CloneID	ODa43205
Clone ID Related Accession (Same CDS sequence)	XM_009296352.2 , XM_009296352.3
Accession Version	XM_009296352.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294627.1
CDS	254..4372
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGN

Target ORF information:

RefSeq Version	XM_009296352.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296352.2

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dot1l ( XM_009296352.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294627.1 Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43205
Clone ID Related Accession (Same CDS sequence)	XM_009296352.2 , XM_009296352.3
Accession Version	XM_009296352.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294627.1
CDS	257..4375
Translation	MGEKLELKLKSPVGAEAAGYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNKRPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQIIDEMEMMEEDTFVDLGSGVGQVVLQVAAATNCKHYYGVEKADIPATYAESMDKEFKRWMKWYGKKHGEYTLERGDFLSEEWKERIASTSVIFVNNFAFGPEVDHQLKERFANMKEGGKIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLRGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEVARRRPQKDSKENKSNTTTPTKPKEHKQAQHDSGGEEEPVVPVKPSPKPRRAKILTRGRKLGNRKRGRPKKAPAATAAERKNKKSQSALDLLHAKTLSAAPAQEPYRSPQSPFYQLPPKVQHYTSSQLLMAPTPPGLQTLLDNIKVQYLHFMAYMKTPQYRTNLQQSLEQEKLRHTELSSQAQQLVNACHAHKERIHDLFQSKLEELGVKAVTVEDLVQAQKEITAHNMQLREQTKQLEHDMAELRDQSQLLLKARCEELKLDTLCWDTLKKEIEALRRQISEKQRHCLELQISIVELEKSQRQQELLQLKSSYSPCEVSPYRKALPGPDPRPTLDPDTPKLTPQSSMGLNGLSPELSINGTASPGYERCTGSAMGKNELLTHYLPISPDHEIVPPTPDSRTRQLGQPLPDYTRFSPAKIALRRHLNQDSAANQFRALGNIHRGDTGAISSPTLGFKQASPCSSEVQAVTTAKSTDRADKEKSPTAPGDTITSLPISIPLSTVHPSKLPVSIPLASVVLPNRVERLRSTLSPVLNPAGQSNGKPPLTPTSGYSSSSGLVNGSHSTMLTEDQDCDVASPPPHSTPVMGLQPRGSGLSPPLGTGGVLQYADGPPRLPPEDGHERQGPESDTEPLDSEARRRIFFSSSSSSSSSSSSTGGSRLHHGSVKQGHHHHSKHHHHHHHHHHHHSPSSHSQEGRKRGRRKRSSSGAVPMSGSPKRRVFPGLGCSSHSSGSPLNINSMVNNINQPLEISAISSPEQSGRSPCEQDLDQPPVLKRERPLELNNTGRYSSAPSSDEEGNGYAPDTSSSSRIERKIATISLDRDGPVRDLERGLQGRKSGASSVSSEVSSSSGTSKWKSTFSPISDPKPTPPDIRQGGSPFSVGASRGGTDSDSDHKPQRRGEREREPYGNSNLFLSQDGSGRSAVNTSERSLQKQKVREWDLKSVSGLASQNLFISAAASGGILSGKVGGAATVSSASAVGQYFPLGGASVLQPLFGTQAAGPATSGAPRLVNGHSALGSFSSAGLAGGAAGGN

Target ORF information:

RefSeq Version	XM_009296352.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DOT1-like histone H3K79 methyltransferase (dot1l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296352.3

ORF Insert Sequence:

ATGGGAGAAA AGCTGGAGTT AAAACTGAAG TCGCCGGTCG GAGCCGAAGC TGCAGGCTAT 
CCGTGGCCCT TACCTGTATA TGATAAACAT CATGATGCTG CACACGAAAT CATCGAAACC 
ATTCGCTGGG TGTGTGAAGA GATCCCAGAT CTTAAGCTGG CCATGGAGAA CTACGTTCTT 
ATTGACTATG ACACCAAGAG CTTCGAGAGT ATGCAGAGAC TATGTGATAA GTACAACAGA 
GCCATCGACA GCATTCACCA ACTGTGGAAA GGGACCACCC AGCCTATGAA ACTGAACAAG 
CGGCCTTCCA ATGGCTTGCT GCGTCACATC CTGCAGCAGG TCTACAACCA CTCTGTCACC 
GACCCGGAAA AACTCAACAA CTACGAGCCA TTTTCTCCAG AGGTATACGG AGAGACCTCC 
TTCGACCTGG TGGCTCAAAT CATCGACGAG ATGGAGATGA TGGAGGAAGA CACCTTTGTG 
GATCTGGGCA GTGGGGTCGG TCAGGTGGTC CTGCAAGTTG CTGCGGCCAC CAATTGTAAA 
CACTACTATG GCGTGGAGAA AGCAGACATT CCAGCTACCT ATGCAGAATC AATGGACAAA 
GAGTTTAAGA GGTGGATGAA GTGGTATGGC AAAAAACATG GCGAGTACAC GCTGGAAAGG 
GGAGATTTCC TTTCAGAAGA ATGGAAGGAA CGAATAGCCA GTACAAGTGT TATTTTTGTG 
AATAACTTTG CCTTTGGTCC TGAGGTGGAC CACCAGTTGA AGGAACGATT CGCCAACATG 
AAGGAAGGTG GAAAAATTGT TTCCTCTAAA CCATTTGCAC CTCTTAACTT CAGAATAAAC 
AGCCGAAACT TGAGTGATAT TGGCACAATA ATGCGAGTTG TGGAGCTGTC CCCTCTGAGG 
GGTTCGGTGT CCTGGACAGG GAAGCCAGTC TCGTACTATC TGCATACAAT AGACCGCACC 
ATACTTGAAA ACTATTTTTC TAGTCTCAAA AATCCTAAAC TCAGGGAGGA ACAGGAGGTT 
GCCAGAAGGC GCCCACAGAA GGACAGTAAA GAGAACAAGA GCAACACCAC CACCCCGACC 
AAGCCTAAAG AGCACAAGCA GGCGCAGCAT GATTCGGGTG GTGAAGAGGA GCCAGTTGTC 
CCCGTGAAGC CGTCACCCAA ACCCCGCCGG GCCAAAATTC TGACCAGGGG CCGCAAGCTT 
GGCAACAGGA AGCGCGGGCG ACCCAAGAAG GCACCAGCTG CAACTGCAGC CGAGCGCAAG 
AACAAGAAGA GCCAGAGCGC CCTCGACCTG CTGCACGCCA AGACCCTCTC AGCGGCCCCT 
GCTCAGGAAC CATACCGGTC ACCTCAGAGT CCGTTCTATC AGCTACCTCC CAAAGTGCAG 
CATTACACAT CCAGCCAGCT CCTCATGGCT CCCACTCCAC CTGGCCTGCA GACGCTACTG 
GATAACATCA AAGTCCAGTA CCTGCACTTC ATGGCCTACA TGAAGACGCC ACAGTACCGC 
ACCAACCTGC AGCAATCGCT TGAGCAGGAG AAGCTGAGAC ACACAGAACT GTCAAGTCAA 
GCCCAGCAGC TGGTCAACGC TTGTCATGCT CACAAGGAGA GGATTCATGA CCTTTTCCAG 
TCCAAACTAG AAGAGCTGGG TGTGAAGGCG GTGACGGTGG AGGATCTGGT TCAGGCTCAG 
AAGGAGATTA CAGCCCACAA CATGCAGCTG CGCGAACAGA CCAAACAGCT GGAGCATGAC 
ATGGCCGAGC TCCGAGATCA GAGCCAGCTG CTGCTGAAGG CTCGCTGTGA GGAGCTCAAG 
CTGGACACTC TGTGTTGGGA CACTCTGAAG AAAGAGATAG AAGCTCTGCG GAGACAGATC 
TCTGAGAAAC AGCGCCACTG TCTGGAGCTC CAGATCAGTA TAGTTGAACT CGAGAAGAGC 
CAGCGACAGC AGGAGCTCCT TCAGCTCAAG TCATCCTACA GCCCTTGTGA AGTGTCCCCC 
TACAGAAAAG CCCTCCCTGG CCCTGATCCT CGGCCCACAC TGGACCCGGA TACCCCAAAA 
CTCACCCCGC AATCCTCTAT GGGGCTGAAC GGCCTCAGTC CTGAACTCTC TATCAACGGC 
ACTGCCTCTC CGGGTTATGA AAGATGCACC GGAAGTGCAA TGGGCAAAAA CGAACTCCTC 
ACCCACTACC TGCCCATTTC TCCAGACCAC GAAATTGTGC CGCCAACCCC GGACTCCAGA 
ACCAGGCAGC TTGGGCAACC CCTGCCCGAC TACACCCGCT TTTCTCCAGC AAAGATCGCC 
CTCCGCAGGC ACCTCAACCA GGATTCAGCC GCAAACCAGT TCCGGGCACT CGGAAACATC 
CATCGGGGCG ACACTGGTGC CATTTCATCT CCAACACTGG GCTTTAAACA GGCTTCACCA 
TGTTCCTCGG AAGTTCAAGC GGTCACCACA GCCAAGAGCA CAGATAGGGC CGATAAAGAA 
AAGAGCCCGA CTGCACCCGG CGACACTATA ACCAGTCTAC CAATCAGCAT CCCTCTCAGC 
ACTGTGCATC CCAGCAAACT TCCTGTCAGC ATCCCTCTGG CCAGTGTGGT GCTGCCGAAC 
CGCGTCGAAA GACTGAGGAG CACTCTTAGT CCGGTATTGA ATCCAGCAGG ACAGAGCAAT 
GGCAAGCCAC CTCTCACGCC TACCTCAGGG TACTCCTCCA GCTCAGGATT GGTTAATGGG 
TCTCACTCCA CCATGCTGAC CGAGGACCAA GACTGTGATG TTGCCTCCCC TCCTCCTCAC 
AGCACTCCCG TCATGGGTCT TCAGCCCCGC GGCTCTGGCC TCAGTCCTCC ACTGGGCACC 
GGAGGCGTTC TCCAGTACGC CGACGGTCCA CCAAGGCTTC CCCCCGAGGA TGGCCATGAG 
CGCCAAGGCC CCGAATCCGA CACAGAGCCC TTAGATAGTG AGGCTCGCCG GCGCATTTTT 
TTCTCTTCAT CCTCCTCTTC TTCCTCATCC TCATCCTCCA CTGGAGGCTC GAGGCTGCAC 
CATGGGTCTG TAAAGCAGGG TCACCATCAT CACAGTAAGC ACCATCACCA CCACCACCAC 
CATCATCACC ATCACTCTCC AAGCTCCCAC AGCCAAGAGG GCCGAAAACG AGGAAGACGA 
AAGCGCAGCT CATCTGGAGC CGTGCCCATG AGTGGATCCC CCAAAAGAAG GGTCTTTCCT 
GGACTGGGCT GCTCAAGTCA TTCCTCTGGA TCACCGCTTA ATATCAATTC AATGGTAAAC 
AACATAAACC AGCCTTTGGA GATCTCCGCC ATTTCTTCCC CAGAGCAGTC AGGCAGAAGC 
CCATGTGAGC AAGATCTGGA TCAGCCACCT GTTTTGAAAC GAGAGCGTCC TCTTGAGCTC 
AACAACACCG GCCGTTATTC CTCTGCCCCA AGTTCAGATG AAGAAGGCAA TGGTTACGCC 
CCGGACACCT CTAGTTCAAG CCGAATTGAA CGAAAAATTG CTACTATTTC CTTAGACCGA 
GATGGACCAG TTAGAGACTT GGAAAGAGGT TTACAGGGAC GTAAATCAGG TGCCAGCAGC 
GTGAGCAGCG AGGTATCCTC TTCCTCCGGA ACCAGCAAGT GGAAATCCAC CTTCTCCCCC 
ATTTCTGATC CCAAGCCAAC TCCTCCAGAC ATCCGCCAAG GTGGCTCACC ATTTAGCGTT 
GGAGCTTCCC GAGGAGGCAC AGACTCAGAC TCGGACCACA AACCCCAGAG GAGAGGGGAG 
CGTGAGCGTG AGCCTTATGG AAATTCAAAT CTCTTCCTCA GTCAGGATGG CAGTGGTCGC 
TCGGCGGTAA ACACGTCAGA GCGTTCGCTG CAGAAACAGA AAGTGCGGGA GTGGGACCTG 
AAATCTGTAA GTGGATTGGC CAGTCAAAAC CTATTTATAT CTGCAGCAGC CAGTGGAGGC 
ATCCTGAGTG GGAAAGTGGG CGGGGCTGCC ACTGTGTCAT CCGCCTCTGC GGTAGGGCAA 
TATTTCCCAC TGGGCGGAGC TTCTGTTCTA CAACCCTTAT TTGGAACTCA GGCAGCGGGA 
CCAGCCACCA GCGGAGCCCC CCGATTGGTC AACGGACATT CGGCGCTTGG CAGTTTCTCC 
AGTGCCGGAC TGGCAGGGGG CGCTGCAGGA GGTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dot1l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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