zmp:0000000660 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zmp:0000000660
Entrez Gene ID	559690
Full Name	zmp:0000000660
Synonyms	arap2
General protein information	Preferred Names zmp:0000000660 Names arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303564.3	XP_009301839.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
XM_017357120.1	XP_017212609.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X2
XM_009303563.2	XP_009301838.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_683053.8	XP_688145.6	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_009303564.2	XP_009301839.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_009303563.3	XP_009301838.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
XM_021478014.1	XP_021333689.1	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARAP2	XP_001135477.1
	ARAP2	XP_001090397.1
Xenopus tropicalis (tropical clawed frog)	arap2	XP_002938697.2
Homo sapiens (human)	ARAP2	NP_056045.2
Canis lupus familiaris (dog)	ARAP2	XP_545958.3
Rattus norvegicus (Norway rat)	Arap2	NP_001100686.1
Gallus gallus (chicken)	ARAP2	XP_426346.4
Bos taurus (cattle)	ARAP2	XP_002688267.2
Danio rerio (zebrafish)	LOC559690	XP_688145.6
Mus musculus (house mouse)	Arap2	NP_848494.2

Gene Ontology

The following zmp:0000000660 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zmp:0000000660 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59801	XM_017357120.1 Latest version!	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa13577	XM_009303563.3 Latest version!	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa13577	XM_009303564.3 Latest version!	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa13577	XM_683053.8 Latest version!	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa13577	XM_021478014.1 Latest version!	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa13577	XM_009303564.2	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa13577	XM_009303563.2	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zmp:0000000660 ( XM_017357120.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212609.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa59801
Clone ID Related Accession (Same CDS sequence)	XM_017357120.1
Accession Version	XM_017357120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGA AGTGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212609.1
CDS	473..4774
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSRSVN

Target ORF information:

RefSeq Version	XM_017357120.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357120.1

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGA AGTGTCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_009303564.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301839.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_009303564.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301839.1
CDS	363..5201
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_009303564.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303564.3

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_009303563.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301838.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.

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CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_009303563.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301838.1
CDS	471..5309
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_009303563.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303563.2

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_683053.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_688145.6 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.

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CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_683053.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683053.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688145.6
CDS	414..5252
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_683053.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683053.8

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_009303564.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301839.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.

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CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_009303564.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301839.1
CDS	370..5208
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_009303564.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303564.2

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_009303563.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301838.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.

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CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_009303563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303563.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301838.1
CDS	464..5302
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_009303563.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303563.3

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zmp:0000000660 ( XM_021478014.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333689.1 Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.

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CloneID	ODa13577
Clone ID Related Accession (Same CDS sequence)	XM_009303564.3 , XM_009303563.2 , XM_683053.8 , XM_009303564.2 , XM_009303563.3 , XM_021478014.1
Accession Version	XM_021478014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333689.1
CDS	134..4972
Translation	MEPNQEITMWLTELHLPQYATSFTLAGYHFLKDLRNLNEEKLLEMDNIPTGHRRRILSSLETLMMEVEGSSVKVPVKRKPIPRPRQVLSKDKQKEASTYQHAQAGNVQMTRQTLPARTTAYSGTMNVGSQTLPRIKCHAASSGSSSTSDNGSSESLENFTEDHVTNKSGGLPADGAALGFQGEMVENEIYEECLFATVPSEPRSTRSFKLRHRPVPEIPEHPFIPSYDRNVAGNNNTDHLTRSEGLILPPSHQKASLERTLSPISPYGELFLFNSAENVLDLSKDEMSNQVKETLEQQKLRHKQTQAKEKVHYNNKQDLSKQHIDDDKNVDENDYDDYSTVQASSFCQQITNEHPTPPTLLSSTGPSAVSAQSQADVNVIYSLAHDDQPGLLKPSVPPRTDLSISPYACFYGICKTATKAGWLDKLSPQGNCVFQRRWVKLEGGNLTYYNSEKEMYSKGLIPLSAVKQIRALGENKFEVVTTLRTFVFRAEKEGDRQDWLEVLQAAVSSQSSVSHQPRKNNTNKSGYVELRGLKGRVYLSLMGTCFRLCKTDQDFNAGLAIAVVDLTAACVKQFERKGLEITTPFKSFCFTVESEHERDEWIEAVQESIVETLCNYEVLEKVWFNKSNRKCADCQAPEPEWASINLCVVICKNCAGQHRFLGPGISKVRSMKLDSSIWTNELIELFLEVGNKNSNSLWVANLPPEDELCKQSSTEQRASFIRRKYKKRKYKKVLNGLNTQEELNKALCAAVVLPDVLQTMALVFSGADVMCATGDPELSTPYLLAQKAGQRLQMEFLYHNKLSDFPKLEAICDSSFAADAPSFMDGFLYCSANAGKATLDRKGKPDMVRRWCTLEGGFLSYYDNEKIATPIGRVDIGDVVSLAINNNQTITETGPVFTFELYLRSQRVLVFGVETSDAHQDWTNAIAKSFVPPRADALLRQDSELIGRLYYKEGHDLYHWRIGWFTLVGSELYFCSGDEDEEEGVLQLKRLQELTVSTHIEGDENIQVLLMVESGRTLYIHCITKQDFALWHSAIQLAAGTDGMALSNQQMNKNDVPIIVDSCIAFVTQYGLCYEGIYQKKGDPGRVAHLIQEFTKDARVVKLRVQEQRLEDVTDTLKSFLSHSEDALLAKELYPFWISALDEQDEKVRVQKYSSYIQSLPKVNRSTLGALLQHLYRIQRCSHINQMSSQRLACVFSSCLFQTEGHTAQETQVVEDLINNYIQLFSVNDEQVRQMEKENSFITRWKDTTFSPAGDLIFEAYLEKKEPEKCCLIKISPNMRSDELAVSTLEMKGMEVKAQDLWTTFEVIENGEMERPLHYKEKVLEQVLEWRSLEDPSSAFLLIKKYSGCKMTDSNSDKLKDFIKGEHLKFKDGSSKLLLGNKFQDRYLVLQDKKLLLYKDIKSTKPEREAPLKAVKCYFGLRRKLKPTSSWGFTVYTEKQQWYFCCKGNDSQQDWVTSIIRMKHGSDLWPRDLKQSTSLPYNLSRRRTSTQSTTQSSNKNQREVKGKSVLTTSTEDSNTQNQMAADEGIRPDSLHKLASAEGTPGVSRLEMVIDDTLQSQRRASLQQDTATLTQISQRKAAIPGRGVQLPQNLINELSTVLNKTGRSQKEAD

Target ORF information:

RefSeq Version	XM_021478014.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zmp:0000000660 (zmp:0000000660), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478014.1

ORF Insert Sequence:

ATGGAGCCCA ACCAGGAGAT CACTATGTGG CTAACTGAAC TGCATTTGCC CCAGTATGCC 
ACCAGCTTTA CCCTGGCCGG CTACCATTTC CTAAAAGACC TCAGGAACCT AAATGAGGAG 
AAGCTTCTGG AGATGGACAA TATCCCCACA GGACATCGGC GGAGGATACT AAGCAGCCTG 
GAGACTCTAA TGATGGAGGT TGAGGGAAGT TCTGTCAAAG TACCTGTAAA GAGAAAGCCA 
ATCCCAAGGC CTAGGCAAGT GCTTTCCAAG GATAAGCAGA AAGAAGCATC AACTTATCAG 
CATGCGCAGG CCGGAAACGT CCAGATGACA AGGCAGACTC TTCCTGCAAG GACTACTGCT 
TATTCAGGGA CGATGAATGT TGGTTCTCAA ACACTTCCCA GGATCAAATG TCACGCTGCT 
TCCTCAGGTT CAAGCTCCAC CTCTGATAAT GGCAGCAGCG AATCACTGGA GAACTTTACA 
GAAGATCATG TTACAAATAA AAGTGGTGGT TTACCTGCAG ATGGAGCTGC TTTAGGGTTT 
CAGGGAGAAA TGGTTGAAAA TGAAATCTAC GAGGAGTGCC TGTTTGCTAC TGTCCCCTCC 
GAGCCGAGGT CCACCCGCTC CTTTAAACTA CGACACAGGC CTGTACCAGA GATCCCTGAA 
CATCCCTTCA TACCTTCTTA TGACCGGAAT GTTGCTGGAA ACAACAACAC TGACCATCTC 
ACAAGATCTG AGGGACTAAT CTTACCCCCA AGCCATCAAA AAGCATCACT GGAAAGGACC 
TTGTCCCCCA TTTCCCCATA TGGAGAACTT TTTCTTTTCA ATTCAGCAGA AAATGTACTG 
GATTTGAGCA AAGATGAAAT GTCCAATCAG GTAAAAGAGA CCCTGGAACA ACAGAAGCTG 
AGACACAAGC AGACACAAGC CAAAGAAAAA GTACATTATA ACAACAAGCA AGACCTTTCC 
AAGCAACACA TCGATGATGA TAAGAATGTT GATGAAAATG ATTATGATGA TTACTCTACT 
GTCCAAGCAT CCAGCTTTTG TCAGCAAATT ACTAATGAAC ATCCCACACC TCCTACTCTT 
CTCTCTAGCA CTGGACCCTC AGCTGTGTCA GCCCAGTCAC AAGCAGATGT AAATGTTATA 
TACAGTTTGG CACACGATGA CCAACCAGGT CTCCTGAAAC CCAGCGTTCC TCCTCGAACT 
GACCTGTCCA TTTCTCCATA CGCCTGCTTC TATGGGATCT GTAAGACTGC AACCAAAGCC 
GGCTGGCTGG ACAAACTCTC CCCTCAAGGG AACTGCGTCT TTCAGAGGAG ATGGGTGAAG 
CTCGAAGGAG GAAATCTCAC CTACTACAAC AGCGAGAAGG AGATGTACTC AAAGGGCCTG 
ATACCCCTTT CAGCAGTTAA ACAAATCCGT GCTTTGGGTG AGAATAAGTT TGAGGTGGTC 
ACTACTTTAA GAACCTTTGT GTTTCGGGCA GAGAAAGAAG GAGATCGTCA GGATTGGCTT 
GAGGTTCTTC AGGCTGCAGT GAGCTCTCAG TCCTCCGTTT CCCATCAGCC TCGCAAAAAT 
AACACAAACA AGAGTGGCTA CGTGGAGCTG CGCGGCCTCA AAGGAAGAGT GTACCTGAGC 
CTCATGGGAA CCTGCTTCAG ACTGTGTAAA ACAGACCAGG ACTTTAATGC CGGACTTGCC 
ATTGCTGTGG TGGATCTGAC GGCTGCGTGT GTAAAGCAGT TTGAGAGGAA AGGATTGGAG 
ATCACTACAC CCTTCAAATC CTTCTGTTTT ACAGTGGAGT CGGAGCATGA GAGAGATGAG 
TGGATCGAGG CTGTTCAGGA GTCCATCGTA GAGACGTTGT GCAATTATGA GGTGCTGGAG 
AAAGTTTGGT TCAACAAGTC CAACAGGAAG TGTGCCGATT GTCAGGCACC TGAGCCTGAA 
TGGGCCTCCA TTAACCTCTG TGTGGTCATC TGCAAGAACT GTGCTGGTCA GCATCGCTTC 
CTGGGTCCAG GTATCTCAAA GGTCCGCAGT ATGAAGCTGG ACAGCAGCAT ATGGACCAAT 
GAGCTCATCG AGCTGTTTTT AGAAGTTGGG AATAAGAATT CCAACAGCCT CTGGGTAGCA 
AATCTTCCCC CAGAGGATGA GCTTTGCAAG CAGTCGTCAA CAGAGCAGCG GGCATCCTTT 
ATTCGCAGGA AGTACAAGAA GAGGAAATAC AAGAAAGTTC TGAATGGTCT AAACACTCAG 
GAGGAACTCA ACAAGGCTCT GTGTGCTGCA GTGGTTCTTC CTGATGTCCT GCAGACCATG 
GCTCTTGTTT TCAGCGGGGC AGATGTGATG TGTGCGACTG GTGACCCTGA GCTTTCCACA 
CCGTATCTAC TAGCTCAGAA AGCAGGCCAG AGGCTGCAGA TGGAGTTCCT CTACCACAAC 
AAGCTCTCAG ATTTTCCTAA GCTGGAAGCA ATATGTGACA GCAGTTTTGC TGCTGATGCT 
CCTTCTTTTA TGGATGGATT TCTCTACTGT TCTGCGAATG CTGGCAAGGC CACACTGGAC 
CGGAAAGGGA AACCAGATAT GGTTCGCCGT TGGTGTACAT TGGAAGGAGG ATTTCTTAGT 
TACTATGACA ACGAGAAGAT CGCCACACCC ATTGGCCGGG TTGACATCGG TGATGTGGTG 
AGCTTGGCTA TCAACAACAA CCAGACCATC ACCGAAACGG GGCCAGTGTT CACCTTTGAG 
TTGTACCTGC GCTCTCAAAG AGTGCTGGTG TTCGGAGTGG AGACCTCGGA CGCACACCAA 
GATTGGACAA ACGCCATAGC CAAGAGTTTT GTCCCTCCAA GGGCAGATGC TCTGCTGAGG 
CAGGACAGTG AGCTGATTGG TCGACTGTAC TATAAAGAGG GTCATGATCT TTATCACTGG 
CGAATCGGAT GGTTTACTCT GGTGGGCTCA GAGCTGTACT TCTGTTCTGG AGATGAAGAT 
GAAGAGGAGG GAGTTCTTCA ACTCAAACGC CTACAGGAGC TGACTGTGTC CACTCATATT 
GAGGGTGACG AGAACATTCA GGTCTTACTT ATGGTGGAGA GTGGAAGGAC TCTGTATATC 
CACTGCATAA CCAAGCAGGA CTTTGCACTG TGGCACTCCG CCATCCAGTT GGCAGCTGGA 
ACGGACGGTA TGGCTCTCAG CAACCAGCAA ATGAATAAAA ATGATGTTCC CATCATTGTG 
GATAGCTGCA TTGCATTTGT CACCCAATAT GGCTTGTGTT ATGAGGGAAT CTACCAGAAG 
AAGGGTGACC CGGGGCGAGT GGCTCACCTC ATCCAGGAAT TTACCAAAGA CGCTCGGGTT 
GTTAAACTTC GGGTTCAAGA GCAGCGACTG GAGGACGTCA CAGACACACT CAAGAGCTTT 
CTGTCCCACA GCGAAGATGC CCTGCTGGCT AAAGAACTAT ACCCTTTTTG GATCTCCGCC 
CTTGATGAAC AGGACGAGAA GGTCAGAGTG CAAAAATATT CCTCCTACAT TCAGAGTTTG 
CCCAAAGTCA ACAGATCAAC ACTCGGTGCC CTGCTGCAGC ATTTGTACAG AATCCAGAGG 
TGCTCACACA TCAACCAGAT GAGCTCACAG AGGCTGGCAT GTGTTTTCTC CTCATGCCTC 
TTTCAGACGG AGGGACACAC AGCTCAAGAG ACTCAAGTGG TGGAAGACCT CATCAACAAT 
TACATCCAGC TTTTTTCTGT CAATGACGAG CAGGTTAGGC AAATGGAGAA AGAAAACAGC 
TTTATCACCA GGTGGAAGGA CACCACATTC TCTCCTGCTG GTGACCTGAT ATTTGAAGCC 
TACCTGGAGA AAAAGGAGCC AGAGAAGTGC TGTTTAATCA AGATCTCTCC GAATATGAGG 
TCAGATGAAC TTGCCGTCAG CACTCTGGAG ATGAAGGGCA TGGAGGTCAA AGCTCAAGAT 
CTCTGGACCA CGTTTGAGGT CATTGAAAAT GGAGAAATGG AGCGGCCCTT ACACTACAAA 
GAGAAGGTTC TGGAGCAAGT TTTGGAGTGG AGATCTCTGG AGGATCCCAG CTCGGCCTTC 
CTGCTCATTA AAAAATATTC TGGGTGTAAA ATGACAGATT CCAACTCAGA TAAACTGAAG 
GATTTCATTA AAGGAGAGCA TCTCAAATTC AAAGACGGTT CTTCCAAACT GCTATTGGGG 
AATAAGTTTC AGGACCGCTA CTTGGTACTA CAAGACAAAA AACTGCTGCT GTACAAAGAC 
ATAAAAAGCA CCAAGCCAGA AAGAGAAGCT CCATTAAAGG CTGTAAAATG CTACTTTGGG 
CTTCGAAGGA AGCTGAAACC AACCAGCAGT TGGGGATTTA CTGTTTATAC TGAAAAGCAG 
CAGTGGTATT TCTGCTGTAA AGGGAATGAT TCTCAGCAGG ACTGGGTCAC CTCCATTATC 
AGAATGAAGC ACGGCAGTGA CTTATGGCCT AGGGACCTAA AGCAGAGCAC ATCTCTACCT 
TATAACCTGA GCAGAAGGAG AACCTCCACT CAGAGCACCA CACAGTCCAG CAATAAAAAC 
CAAAGAGAGG TTAAAGGAAA GTCGGTGCTT ACTACTAGTA CAGAAGATTC AAATACACAA 
AACCAAATGG CCGCAGATGA GGGCATCCGA CCGGACTCGC TGCATAAATT GGCAAGTGCT 
GAAGGAACTC CGGGTGTCTC CAGGCTTGAA ATGGTGATTG ATGACACACT CCAGTCCCAA 
CGAAGGGCAT CACTTCAACA AGACACTGCC ACTCTAACCC AGATTTCACA AAGGAAAGCC 
GCTATTCCCG GCAGAGGAGT GCAGTTGCCT CAAAACCTCA TTAATGAGCT AAGTACTGTT 
CTCAACAAAA CAGGACGCTC TCAGAAAGAA GCCGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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zmp:0000000660 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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