frs2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	frs2a
Entrez Gene ID	559479
Full Name	fibroblast growth factor receptor substrate 2a
Synonyms	si:dkey-97m3.4
General protein information	Preferred Names fibroblast growth factor receptor substrate 2a Names fibroblast growth factor receptor substrate 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017355028.2	XP_017210517.1	fibroblast growth factor receptor substrate 2 isoform X1
XM_017355028.1	XP_017210517.1	fibroblast growth factor receptor substrate 2 isoform X1
XM_005164455.3	XP_005164512.1	fibroblast growth factor receptor substrate 2 isoform X2
NM_001044898.1	NP_001038363.1	fibroblast growth factor receptor substrate 2
XM_005164455.4	XP_005164512.1	fibroblast growth factor receptor substrate 2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FRS2	XP_001155588.1
	LOC717117	XP_001108342.2
Canis lupus familiaris (dog)	FRS2	XP_005625670.1
Homo sapiens (human)	FRS2	NP_001265285.1
Mus musculus (house mouse)	Frs2	NP_808466.1
Rattus norvegicus (Norway rat)	Frs2	NP_001101567.2
Gallus gallus (chicken)	FRS2	NP_001186555.1
Danio rerio (zebrafish)	frs2a	NP_001038363.1
Xenopus tropicalis (tropical clawed frog)	frs2	NP_001128281.1
Bos taurus (cattle)	FRS2	XP_002687431.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Trans-Activation between EphA and FGFR Regulates Self-Renewal and Differentiation of Mouse Embryonic Neural Stem/Progenitor Cells via Differential Activation of FRS2?.
Sawada T, Arai D, Jing X, Furushima K, Chen Q, Kawakami K, Yokote H, Miyajima M, Sakaguchi K
PloS one10(5)e0128826(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that the ternary complex of EphA, FGFR and FRS2alpha formed by ligand stimulation regulates self-renewal and differentiation of mouse embryonic neural stem/progenitor cells
Title: Trans-Activation between EphA and FGFR Regulates Self-Renewal and Differentiation of Mouse Embryonic Neural Stem/Progenitor Cells via Differential Activation of FRS2?.

The following frs2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frs2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09029	NM_001044898.1 Latest version!	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa09029	XM_005164455.4 Latest version!	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa58380	XM_017355028.2 Latest version!	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa58380	XM_017355028.1	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa09029	XM_005164455.3	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frs2a ( NM_001044898.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038363.1 Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), mRNA.

Price & Availability↑

CloneID	ODa09029
Clone ID Related Accession (Same CDS sequence)	XM_005164455.3 , NM_001044898.1 , XM_005164455.4
Accession Version	NM_001044898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	fibroblast growth factor receptor substrate 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR749743.13. On or before Sep 1, 2006 this sequence version replaced XM_678460.1, XM_702009.1. ##Evidence-Data-START## CDS exon combination :: GDQH01023536.1, GDQQ01014918.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038363.1
CDS	1..1458
Translation	MGSCCSCPDKEIIPDNHHNKFKVINVDDDGNELGSGVMELTEEELILHTRKRDAVKWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEEIFNMLQDIMHNNSISVVEEPVLEPELPATPHTPTTPGYSVPTVANGISRYPLFDASSRPSSRHPSVGSTRLPSVGEESTHPLLLNDETVHTYVNTTGLQEERRCRSTGHTPLEPETSNHESAVPASPNEQEPCVVLEPESVKFVLGPTPVQRQRQQQETGEEATNSEHTSYTPPVKTVNGNAGLMADCDTGYDSDERKETSCEHHNGISTGARRIRPPVPLPDAHNANNSAQRRTALLNYENLPALPPVWEMRKPEEDELKSPSLNGFHSLDPQHNYVNTENVTVPLSAHKHNSQPSVFNFDLRRLGVGDPLRQLNYIEVEMDKVSDSSGPHTPKTPTTPLPATPTRRTELYAVIDIERTAAMSSLQKAQPRDDGTSRKTRHNSTDLPM

Target ORF information:

RefSeq Version	NM_001044898.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044898.1

ORF Insert Sequence:

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frs2a ( XM_005164455.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164512.1 Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa09029
Clone ID Related Accession (Same CDS sequence)	XM_005164455.3 , NM_001044898.1 , XM_005164455.4
Accession Version	XM_005164455.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	fibroblast growth factor receptor substrate 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164455.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164512.1
CDS	490..1947
Translation	MGSCCSCPDKEIIPDNHHNKFKVINVDDDGNELGSGVMELTEEELILHTRKRDAVKWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEEIFNMLQDIMHNNSISVVEEPVLEPELPATPHTPTTPGYSVPTVANGISRYPLFDASSRPSSRHPSVGSTRLPSVGEESTHPLLLNDETVHTYVNTTGLQEERRCRSTGHTPLEPETSNHESAVPASPNEQEPCVVLEPESVKFVLGPTPVQRQRQQQETGEEATNSEHTSYTPPVKTVNGNAGLMADCDTGYDSDERKETSCEHHNGISTGARRIRPPVPLPDAHNANNSAQRRTALLNYENLPALPPVWEMRKPEEDELKSPSLNGFHSLDPQHNYVNTENVTVPLSAHKHNSQPSVFNFDLRRLGVGDPLRQLNYIEVEMDKVSDSSGPHTPKTPTTPLPATPTRRTELYAVIDIERTAAMSSLQKAQPRDDGTSRKTRHNSTDLPM

Target ORF information:

RefSeq Version	XM_005164455.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164455.3

ORF Insert Sequence:

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frs2a ( XM_005164455.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164512.1 Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa09029
Clone ID Related Accession (Same CDS sequence)	XM_005164455.3 , NM_001044898.1 , XM_005164455.4
Accession Version	XM_005164455.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	fibroblast growth factor receptor substrate 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164455.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164512.1
CDS	499..1956
Translation	MGSCCSCPDKEIIPDNHHNKFKVINVDDDGNELGSGVMELTEEELILHTRKRDAVKWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEEIFNMLQDIMHNNSISVVEEPVLEPELPATPHTPTTPGYSVPTVANGISRYPLFDASSRPSSRHPSVGSTRLPSVGEESTHPLLLNDETVHTYVNTTGLQEERRCRSTGHTPLEPETSNHESAVPASPNEQEPCVVLEPESVKFVLGPTPVQRQRQQQETGEEATNSEHTSYTPPVKTVNGNAGLMADCDTGYDSDERKETSCEHHNGISTGARRIRPPVPLPDAHNANNSAQRRTALLNYENLPALPPVWEMRKPEEDELKSPSLNGFHSLDPQHNYVNTENVTVPLSAHKHNSQPSVFNFDLRRLGVGDPLRQLNYIEVEMDKVSDSSGPHTPKTPTTPLPATPTRRTELYAVIDIERTAAMSSLQKAQPRDDGTSRKTRHNSTDLPM

Target ORF information:

RefSeq Version	XM_005164455.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164455.4

ORF Insert Sequence:

ATGGGGAGCT GCTGTAGCTG TCCCGATAAA GAGATCATCC CTGACAACCA TCACAACAAA 
TTCAAGGTGA TAAACGTGGA TGATGATGGT AATGAGCTGG GCTCAGGTGT GATGGAGTTA 
ACAGAGGAAG AGCTCATTCT GCACACGCGC AAACGAGACG CCGTCAAGTG GCCGTATCTC 
TGTCTGCGCC GCTATGGGTA CGACTCAAAC CTCTTCTCCT TCGAGAGTGG CCGGCGCTGC 
CAGACTGGAC AAGGGATATT TGCGTTCAAA TGCGCTCGGG CCGAGGAGAT CTTCAACATG 
CTGCAGGACA TCATGCACAA CAACAGCATC AGTGTGGTGG AGGAGCCTGT GCTGGAGCCT 
GAGCTTCCTG CGACGCCACA CACACCCACC ACTCCAGGTT ACTCTGTCCC GACGGTGGCC 
AATGGGATCA GCCGCTATCC ATTGTTTGAC GCCTCCTCGC GGCCGTCCAG TCGCCATCCT 
TCTGTGGGCA GCACTAGACT ACCATCAGTA GGAGAAGAGT CGACACATCC ACTGCTGCTC 
AACGACGAAA CGGTACACAC ATACGTCAAC ACTACAGGAC TGCAGGAGGA GCGGCGCTGT 
CGCAGCACAG GCCACACCCC TCTGGAGCCT GAGACGTCCA ATCACGAGAG CGCAGTGCCA 
GCGTCGCCCA ATGAGCAGGA GCCTTGTGTG GTGCTGGAGC CTGAGAGTGT GAAGTTTGTT 
TTAGGCCCGA CGCCTGTGCA GAGACAGAGA CAGCAGCAGG AGACGGGCGA GGAGGCGACT 
AATTCTGAAC ACACTTCATA CACTCCTCCT GTTAAGACAG TAAATGGCAA CGCAGGCCTT 
ATGGCGGACT GTGACACCGG CTACGACAGC GATGAGCGCA AAGAGACATC ATGTGAGCAT 
CATAACGGCA TTTCAACAGG AGCCAGACGC ATCAGACCCC CCGTCCCGCT GCCAGATGCA 
CACAATGCTA ACAATTCAGC CCAGCGGCGG ACAGCGCTAC TAAACTATGA GAATCTGCCT 
GCATTGCCGC CTGTCTGGGA GATGCGCAAA CCCGAGGAGG ATGAACTCAA ATCTCCATCG 
CTGAACGGAT TTCATTCACT CGACCCGCAG CATAATTACG TCAATACTGA GAATGTGACG 
GTGCCGCTTA GTGCACATAA ACACAACTCT CAGCCGAGCG TTTTTAACTT TGACCTGCGG 
CGGCTTGGAG TGGGCGACCC GCTCCGCCAG CTGAACTACA TTGAGGTGGA AATGGACAAA 
GTGTCGGATT CTAGCGGGCC TCATACGCCT AAAACACCCA CTACACCTCT GCCGGCCACT 
CCTACACGGA GAACTGAGCT GTACGCTGTT ATTGATATTG AACGCACTGC TGCCATGTCC 
AGCCTGCAGA AAGCACAGCC ACGGGACGAT GGGACGTCCA GAAAAACACG ACACAACAGC 
ACCGACCTGC CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frs2a ( XM_017355028.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210517.1 Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58380
Clone ID Related Accession (Same CDS sequence)	XM_017355028.2 , XM_017355028.1
Accession Version	XM_017355028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	fibroblast growth factor receptor substrate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT GGGTCCAGTG GTGTGTTAAA GGCGCAATGG GGAGCTGCTG TAGCTGTCCC 
GATAAAGAGA TCATCCCTGA CAACCATCAC AACAAATTCA AGGTGATAAA CGTGGATGAT 
GATGGTAATG AGCTGGGCTC AGGTGTGATG GAGTTAACAG AGGAAGAGCT CATTCTGCAC 
ACGCGCAAAC GAGACGCCGT CAAGTGGCCG TATCTCTGTC TGCGCCGCTA TGGGTACGAC 
TCAAACCTCT TCTCCTTCGA GAGTGGCCGG CGCTGCCAGA CTGGACAAGG GATATTTGCG 
TTCAAATGCG CTCGGGCCGA GGAGATCTTC AACATGCTGC AGGACATCAT GCACAACAAC 
AGCATCAGTG TGGTGGAGGA GCCTGTGCTG GAGCCTGAGC TTCCTGCGAC GCCACACACA 
CCCACCACTC CAGGTTACTC TGTCCCGACG GTGGCCAATG GGATCAGCCG CTATCCATTG 
TTTGACGCCT CCTCGCGGCC GTCCAGTCGC CATCCTTCTG TGGGCAGCAC TAGACTACCA 
TCAGTAGGAG AAGAGTCGAC ACATCCACTG CTGCTCAACG ACGAAACGGT ACACACATAC 
GTCAACACTA CAGGACTGCA GGAGGAGCGG CGCTGTCGCA GCACAGGCCA CACCCCTCTG 
GAGCCTGAGA CGTCCAATCA CGAGAGCGCA GTGCCAGCGT CGCCCAATGA GCAGGAGCCT 
TGTGTGGTGC TGGAGCCTGA GAGTGTGAAG TTTGTTTTAG GCCCGACGCC TGTGCAGAGA 
CAGAGACAGC AGCAGGAGAC GGGCGAGGAG GCGACTAATT CTGAACACAC TTCATACACT 
CCTCCTGTTA AGACAGTAAA TGGCAACGCA GGCCTTATGG CGGACTGTGA CACCGGCTAC 
GACAGCGATG AGCGCAAAGA GACATCATGT GAGCATCATA ACGGCATTTC AACAGGAGCC 
AGACGCATCA GACCCCCCGT CCCGCTGCCA GATGCACACA ATGCTAACAA TTCAGCCCAG 
CGGCGGACAG CGCTACTAAA CTATGAGAAT CTGCCTGCAT TGCCGCCTGT CTGGGAGATG 
CGCAAACCCG AGGAGGATGA ACTCAAATCT CCATCGCTGA ACGGATTTCA TTCACTCGAC 
CCGCAGCATA ATTACGTCAA TACTGAGAAT GTGACGGTGC CGCTTAGTGC ACATAAACAC 
AACTCTCAGC CGAGCGTTTT TAACTTTGAC CTGCGGCGGC TTGGAGTGGG CGACCCGCTC 
CGCCAGCTGA ACTACATTGA GGTGGAAATG GACAAAGTGT CGGATTCTAG CGGGCCTCAT 
ACGCCTAAAA CACCCACTAC ACCTCTGCCG GCCACTCCTA CACGGAGAAC TGAGCTGTAC 
GCTGTTATTG ATATTGAACG CACTGCTGCC ATGTCCAGCC TGCAGAAAGC ACAGCCACGG 
GACGATGGGA CGTCCAGAAA AACACGACAC AACAGCACCG ACCTGCCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210517.1
CDS	460..1953
Translation	MAGWVQWCVKGAMGSCCSCPDKEIIPDNHHNKFKVINVDDDGNELGSGVMELTEEELILHTRKRDAVKWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEEIFNMLQDIMHNNSISVVEEPVLEPELPATPHTPTTPGYSVPTVANGISRYPLFDASSRPSSRHPSVGSTRLPSVGEESTHPLLLNDETVHTYVNTTGLQEERRCRSTGHTPLEPETSNHESAVPASPNEQEPCVVLEPESVKFVLGPTPVQRQRQQQETGEEATNSEHTSYTPPVKTVNGNAGLMADCDTGYDSDERKETSCEHHNGISTGARRIRPPVPLPDAHNANNSAQRRTALLNYENLPALPPVWEMRKPEEDELKSPSLNGFHSLDPQHNYVNTENVTVPLSAHKHNSQPSVFNFDLRRLGVGDPLRQLNYIEVEMDKVSDSSGPHTPKTPTTPLPATPTRRTELYAVIDIERTAAMSSLQKAQPRDDGTSRKTRHNSTDLPM

Target ORF information:

RefSeq Version	XM_017355028.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355028.2

ORF Insert Sequence:

ATGGCTGGAT GGGTCCAGTG GTGTGTTAAA GGCGCAATGG GGAGCTGCTG TAGCTGTCCC 
GATAAAGAGA TCATCCCTGA CAACCATCAC AACAAATTCA AGGTGATAAA CGTGGATGAT 
GATGGTAATG AGCTGGGCTC AGGTGTGATG GAGTTAACAG AGGAAGAGCT CATTCTGCAC 
ACGCGCAAAC GAGACGCCGT CAAGTGGCCG TATCTCTGTC TGCGCCGCTA TGGGTACGAC 
TCAAACCTCT TCTCCTTCGA GAGTGGCCGG CGCTGCCAGA CTGGACAAGG GATATTTGCG 
TTCAAATGCG CTCGGGCCGA GGAGATCTTC AACATGCTGC AGGACATCAT GCACAACAAC 
AGCATCAGTG TGGTGGAGGA GCCTGTGCTG GAGCCTGAGC TTCCTGCGAC GCCACACACA 
CCCACCACTC CAGGTTACTC TGTCCCGACG GTGGCCAATG GGATCAGCCG CTATCCATTG 
TTTGACGCCT CCTCGCGGCC GTCCAGTCGC CATCCTTCTG TGGGCAGCAC TAGACTACCA 
TCAGTAGGAG AAGAGTCGAC ACATCCACTG CTGCTCAACG ACGAAACGGT ACACACATAC 
GTCAACACTA CAGGACTGCA GGAGGAGCGG CGCTGTCGCA GCACAGGCCA CACCCCTCTG 
GAGCCTGAGA CGTCCAATCA CGAGAGCGCA GTGCCAGCGT CGCCCAATGA GCAGGAGCCT 
TGTGTGGTGC TGGAGCCTGA GAGTGTGAAG TTTGTTTTAG GCCCGACGCC TGTGCAGAGA 
CAGAGACAGC AGCAGGAGAC GGGCGAGGAG GCGACTAATT CTGAACACAC TTCATACACT 
CCTCCTGTTA AGACAGTAAA TGGCAACGCA GGCCTTATGG CGGACTGTGA CACCGGCTAC 
GACAGCGATG AGCGCAAAGA GACATCATGT GAGCATCATA ACGGCATTTC AACAGGAGCC 
AGACGCATCA GACCCCCCGT CCCGCTGCCA GATGCACACA ATGCTAACAA TTCAGCCCAG 
CGGCGGACAG CGCTACTAAA CTATGAGAAT CTGCCTGCAT TGCCGCCTGT CTGGGAGATG 
CGCAAACCCG AGGAGGATGA ACTCAAATCT CCATCGCTGA ACGGATTTCA TTCACTCGAC 
CCGCAGCATA ATTACGTCAA TACTGAGAAT GTGACGGTGC CGCTTAGTGC ACATAAACAC 
AACTCTCAGC CGAGCGTTTT TAACTTTGAC CTGCGGCGGC TTGGAGTGGG CGACCCGCTC 
CGCCAGCTGA ACTACATTGA GGTGGAAATG GACAAAGTGT CGGATTCTAG CGGGCCTCAT 
ACGCCTAAAA CACCCACTAC ACCTCTGCCG GCCACTCCTA CACGGAGAAC TGAGCTGTAC 
GCTGTTATTG ATATTGAACG CACTGCTGCC ATGTCCAGCC TGCAGAAAGC ACAGCCACGG 
GACGATGGGA CGTCCAGAAA AACACGACAC AACAGCACCG ACCTGCCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frs2a ( XM_017355028.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210517.1 Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa58380
Clone ID Related Accession (Same CDS sequence)	XM_017355028.2 , XM_017355028.1
Accession Version	XM_017355028.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	fibroblast growth factor receptor substrate 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAT GGGTCCAGTG GTGTGTTAAA GGCGCAATGG GGAGCTGCTG TAGCTGTCCC 
GATAAAGAGA TCATCCCTGA CAACCATCAC AACAAATTCA AGGTGATAAA CGTGGATGAT 
GATGGTAATG AGCTGGGCTC AGGTGTGATG GAGTTAACAG AGGAAGAGCT CATTCTGCAC 
ACGCGCAAAC GAGACGCCGT CAAGTGGCCG TATCTCTGTC TGCGCCGCTA TGGGTACGAC 
TCAAACCTCT TCTCCTTCGA GAGTGGCCGG CGCTGCCAGA CTGGACAAGG GATATTTGCG 
TTCAAATGCG CTCGGGCCGA GGAGATCTTC AACATGCTGC AGGACATCAT GCACAACAAC 
AGCATCAGTG TGGTGGAGGA GCCTGTGCTG GAGCCTGAGC TTCCTGCGAC GCCACACACA 
CCCACCACTC CAGGTTACTC TGTCCCGACG GTGGCCAATG GGATCAGCCG CTATCCATTG 
TTTGACGCCT CCTCGCGGCC GTCCAGTCGC CATCCTTCTG TGGGCAGCAC TAGACTACCA 
TCAGTAGGAG AAGAGTCGAC ACATCCACTG CTGCTCAACG ACGAAACGGT ACACACATAC 
GTCAACACTA CAGGACTGCA GGAGGAGCGG CGCTGTCGCA GCACAGGCCA CACCCCTCTG 
GAGCCTGAGA CGTCCAATCA CGAGAGCGCA GTGCCAGCGT CGCCCAATGA GCAGGAGCCT 
TGTGTGGTGC TGGAGCCTGA GAGTGTGAAG TTTGTTTTAG GCCCGACGCC TGTGCAGAGA 
CAGAGACAGC AGCAGGAGAC GGGCGAGGAG GCGACTAATT CTGAACACAC TTCATACACT 
CCTCCTGTTA AGACAGTAAA TGGCAACGCA GGCCTTATGG CGGACTGTGA CACCGGCTAC 
GACAGCGATG AGCGCAAAGA GACATCATGT GAGCATCATA ACGGCATTTC AACAGGAGCC 
AGACGCATCA GACCCCCCGT CCCGCTGCCA GATGCACACA ATGCTAACAA TTCAGCCCAG 
CGGCGGACAG CGCTACTAAA CTATGAGAAT CTGCCTGCAT TGCCGCCTGT CTGGGAGATG 
CGCAAACCCG AGGAGGATGA ACTCAAATCT CCATCGCTGA ACGGATTTCA TTCACTCGAC 
CCGCAGCATA ATTACGTCAA TACTGAGAAT GTGACGGTGC CGCTTAGTGC ACATAAACAC 
AACTCTCAGC CGAGCGTTTT TAACTTTGAC CTGCGGCGGC TTGGAGTGGG CGACCCGCTC 
CGCCAGCTGA ACTACATTGA GGTGGAAATG GACAAAGTGT CGGATTCTAG CGGGCCTCAT 
ACGCCTAAAA CACCCACTAC ACCTCTGCCG GCCACTCCTA CACGGAGAAC TGAGCTGTAC 
GCTGTTATTG ATATTGAACG CACTGCTGCC ATGTCCAGCC TGCAGAAAGC ACAGCCACGG 
GACGATGGGA CGTCCAGAAA AACACGACAC AACAGCACCG ACCTGCCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210517.1
CDS	450..1943
Translation	MAGWVQWCVKGAMGSCCSCPDKEIIPDNHHNKFKVINVDDDGNELGSGVMELTEEELILHTRKRDAVKWPYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEEIFNMLQDIMHNNSISVVEEPVLEPELPATPHTPTTPGYSVPTVANGISRYPLFDASSRPSSRHPSVGSTRLPSVGEESTHPLLLNDETVHTYVNTTGLQEERRCRSTGHTPLEPETSNHESAVPASPNEQEPCVVLEPESVKFVLGPTPVQRQRQQQETGEEATNSEHTSYTPPVKTVNGNAGLMADCDTGYDSDERKETSCEHHNGISTGARRIRPPVPLPDAHNANNSAQRRTALLNYENLPALPPVWEMRKPEEDELKSPSLNGFHSLDPQHNYVNTENVTVPLSAHKHNSQPSVFNFDLRRLGVGDPLRQLNYIEVEMDKVSDSSGPHTPKTPTTPLPATPTRRTELYAVIDIERTAAMSSLQKAQPRDDGTSRKTRHNSTDLPM

Target ORF information:

RefSeq Version	XM_017355028.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio fibroblast growth factor receptor substrate 2a (frs2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355028.1

ORF Insert Sequence:

ATGGCTGGAT GGGTCCAGTG GTGTGTTAAA GGCGCAATGG GGAGCTGCTG TAGCTGTCCC 
GATAAAGAGA TCATCCCTGA CAACCATCAC AACAAATTCA AGGTGATAAA CGTGGATGAT 
GATGGTAATG AGCTGGGCTC AGGTGTGATG GAGTTAACAG AGGAAGAGCT CATTCTGCAC 
ACGCGCAAAC GAGACGCCGT CAAGTGGCCG TATCTCTGTC TGCGCCGCTA TGGGTACGAC 
TCAAACCTCT TCTCCTTCGA GAGTGGCCGG CGCTGCCAGA CTGGACAAGG GATATTTGCG 
TTCAAATGCG CTCGGGCCGA GGAGATCTTC AACATGCTGC AGGACATCAT GCACAACAAC 
AGCATCAGTG TGGTGGAGGA GCCTGTGCTG GAGCCTGAGC TTCCTGCGAC GCCACACACA 
CCCACCACTC CAGGTTACTC TGTCCCGACG GTGGCCAATG GGATCAGCCG CTATCCATTG 
TTTGACGCCT CCTCGCGGCC GTCCAGTCGC CATCCTTCTG TGGGCAGCAC TAGACTACCA 
TCAGTAGGAG AAGAGTCGAC ACATCCACTG CTGCTCAACG ACGAAACGGT ACACACATAC 
GTCAACACTA CAGGACTGCA GGAGGAGCGG CGCTGTCGCA GCACAGGCCA CACCCCTCTG 
GAGCCTGAGA CGTCCAATCA CGAGAGCGCA GTGCCAGCGT CGCCCAATGA GCAGGAGCCT 
TGTGTGGTGC TGGAGCCTGA GAGTGTGAAG TTTGTTTTAG GCCCGACGCC TGTGCAGAGA 
CAGAGACAGC AGCAGGAGAC GGGCGAGGAG GCGACTAATT CTGAACACAC TTCATACACT 
CCTCCTGTTA AGACAGTAAA TGGCAACGCA GGCCTTATGG CGGACTGTGA CACCGGCTAC 
GACAGCGATG AGCGCAAAGA GACATCATGT GAGCATCATA ACGGCATTTC AACAGGAGCC 
AGACGCATCA GACCCCCCGT CCCGCTGCCA GATGCACACA ATGCTAACAA TTCAGCCCAG 
CGGCGGACAG CGCTACTAAA CTATGAGAAT CTGCCTGCAT TGCCGCCTGT CTGGGAGATG 
CGCAAACCCG AGGAGGATGA ACTCAAATCT CCATCGCTGA ACGGATTTCA TTCACTCGAC 
CCGCAGCATA ATTACGTCAA TACTGAGAAT GTGACGGTGC CGCTTAGTGC ACATAAACAC 
AACTCTCAGC CGAGCGTTTT TAACTTTGAC CTGCGGCGGC TTGGAGTGGG CGACCCGCTC 
CGCCAGCTGA ACTACATTGA GGTGGAAATG GACAAAGTGT CGGATTCTAG CGGGCCTCAT 
ACGCCTAAAA CACCCACTAC ACCTCTGCCG GCCACTCCTA CACGGAGAAC TGAGCTGTAC 
GCTGTTATTG ATATTGAACG CACTGCTGCC ATGTCCAGCC TGCAGAAAGC ACAGCCACGG 
GACGATGGGA CGTCCAGAAA AACACGACAC AACAGCACCG ACCTGCCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Trans-Activation between EphA and FGFR Regulates Self-Renewal and Differentiation of Mouse Embryonic Neural Stem/Progenitor Cells via Differential Activation of FRS2?.
PloS one10(5)e0128826(2015)
Sawada T,Arai D,Jing X,Furushima K,Chen Q,Kawakami K,Yokote H,Miyajima M,Sakaguchi K

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frs2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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