usp49 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp49
Entrez Gene ID	559473
Full Name	ubiquitin specific peptidase 49
Synonyms	si:rp71-11b17.7
General protein information	Preferred Names ubiquitin specific peptidase 49 Names ubiquitin carboxyl-terminal hydrolase 49
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168272.4	XP_005168329.1	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
XM_005168272.3	XP_005168329.1	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
NM_001044896.1	NP_001038361.1	ubiquitin carboxyl-terminal hydrolase 49
XM_009295758.2	XP_009294033.1	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
XM_009295758.3	XP_009294033.1	ubiquitin carboxyl-terminal hydrolase 49 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP49	NP_001273483.1
Bos taurus (cattle)	USP49	XP_005223589.1
Mus musculus (house mouse)	Usp49	NP_940813.1
Rattus norvegicus (Norway rat)	Usp49	NP_001129942.1
Pan troglodytes (chimpanzee)	USP49	XP_518467.3
Canis lupus familiaris (dog)	USP49	XP_532134.3
Xenopus tropicalis (tropical clawed frog)	usp49	XP_004910733.1
	USP49	XP_001085366.1
Gallus gallus (chicken)	USP49	XP_001231682.1
Danio rerio (zebrafish)	usp49	NP_001038361.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp49 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp49 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa26322	NM_001044896.1 Latest version!	Danio rerio ubiquitin specific peptidase 49 (usp49), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26322	XM_005168272.4 Latest version!	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26322	XM_009295758.3 Latest version!	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa26322	XM_005168272.3	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26322	XM_009295758.2	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp49 ( NM_001044896.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038361.1 Danio rerio ubiquitin specific peptidase 49 (usp49), mRNA.

Price & Availability↑

CloneID	ODa26322
Clone ID Related Accession (Same CDS sequence)	XM_005168272.4 , XM_005168272.3 , NM_001044896.1 , XM_009295758.2 , XM_009295758.3
Accession Version	NM_001044896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 49
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645789.14. On or before Sep 1, 2006 this sequence version replaced XM_681888.1, XM_703518.1, XM_703519.1. ##Evidence-Data-START## Transcript exon combination :: BC171546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038361.1
CDS	1..1950
Translation	MDRCKHVVRFRLGHGHSILNPQMWRCVDCDTTESVWACLKCTHVACGRYMEEHSRSHYQQTQHPLAMDVRELDVFCFACGDYVLNDNVEGDLKLLRGALSTVRSPGQRSLRSSVTDGGLRVCEITRDGAMQTALWHRRKTLLKSALRYWISRQQEEHRKREEKQEQEREEKRRQRKEVKKRLLGELANVPPRKSARLLTQAPRPTIALIPRKFRDPPERLPAAPKQLLTLSAKTLSRKPLRRTARLHRYHASQSARRRKLAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKSNHPQVGALSAGGGGGGGGVPSSLQSAEQKEPRSSSRQQVSLCHELHTLFRVMWSGRWSLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELESDAPRKHFLIPITQRKLSKQVLKVLNTIFHGQLLSQVTCLSCKRKSNTVEPFWDLSLEFPERYHRMEKPSAAACQRSCSLSEMLAKFTETEALEGCIYACNFCNRKRRKSSHKPLSLSEACKQLLICRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIQPYCSCSDAAAREAYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMNVCSVEEVCSTQAYILFYTQRSS

Target ORF information:

RefSeq Version	NM_001044896.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 49 (usp49), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044896.1

ORF Insert Sequence:

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp49 ( XM_005168272.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168329.1 Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26322
Clone ID Related Accession (Same CDS sequence)	XM_005168272.4 , XM_005168272.3 , NM_001044896.1 , XM_009295758.2 , XM_009295758.3
Accession Version	XM_005168272.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168272.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168329.1
CDS	1706..3655
Translation	MDRCKHVVRFRLGHGHSILNPQMWRCVDCDTTESVWACLKCTHVACGRYMEEHSRSHYQQTQHPLAMDVRELDVFCFACGDYVLNDNVEGDLKLLRGALSTVRSPGQRSLRSSVTDGGLRVCEITRDGAMQTALWHRRKTLLKSALRYWISRQQEEHRKREEKQEQEREEKRRQRKEVKKRLLGELANVPPRKSARLLTQAPRPTIALIPRKFRDPPERLPAAPKQLLTLSAKTLSRKPLRRTARLHRYHASQSARRRKLAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKSNHPQVGALSAGGGGGGGGVPSSLQSAEQKEPRSSSRQQVSLCHELHTLFRVMWSGRWSLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELESDAPRKHFLIPITQRKLSKQVLKVLNTIFHGQLLSQVTCLSCKRKSNTVEPFWDLSLEFPERYHRMEKPSAAACQRSCSLSEMLAKFTETEALEGCIYACNFCNRKRRKSSHKPLSLSEACKQLLICRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIQPYCSCSDAAAREAYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMNVCSVEEVCSTQAYILFYTQRSS

Target ORF information:

RefSeq Version	XM_005168272.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168272.4

ORF Insert Sequence:

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp49 ( XM_005168272.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168329.1 Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26322
Clone ID Related Accession (Same CDS sequence)	XM_005168272.4 , XM_005168272.3 , NM_001044896.1 , XM_009295758.2 , XM_009295758.3
Accession Version	XM_005168272.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168272.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168329.1
CDS	1706..3655
Translation	MDRCKHVVRFRLGHGHSILNPQMWRCVDCDTTESVWACLKCTHVACGRYMEEHSRSHYQQTQHPLAMDVRELDVFCFACGDYVLNDNVEGDLKLLRGALSTVRSPGQRSLRSSVTDGGLRVCEITRDGAMQTALWHRRKTLLKSALRYWISRQQEEHRKREEKQEQEREEKRRQRKEVKKRLLGELANVPPRKSARLLTQAPRPTIALIPRKFRDPPERLPAAPKQLLTLSAKTLSRKPLRRTARLHRYHASQSARRRKLAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKSNHPQVGALSAGGGGGGGGVPSSLQSAEQKEPRSSSRQQVSLCHELHTLFRVMWSGRWSLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELESDAPRKHFLIPITQRKLSKQVLKVLNTIFHGQLLSQVTCLSCKRKSNTVEPFWDLSLEFPERYHRMEKPSAAACQRSCSLSEMLAKFTETEALEGCIYACNFCNRKRRKSSHKPLSLSEACKQLLICRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIQPYCSCSDAAAREAYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMNVCSVEEVCSTQAYILFYTQRSS

Target ORF information:

RefSeq Version	XM_005168272.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168272.3

ORF Insert Sequence:

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp49 ( XM_009295758.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294033.1 Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26322
Clone ID Related Accession (Same CDS sequence)	XM_005168272.4 , XM_005168272.3 , NM_001044896.1 , XM_009295758.2 , XM_009295758.3
Accession Version	XM_009295758.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294033.1
CDS	1378..3327
Translation	MDRCKHVVRFRLGHGHSILNPQMWRCVDCDTTESVWACLKCTHVACGRYMEEHSRSHYQQTQHPLAMDVRELDVFCFACGDYVLNDNVEGDLKLLRGALSTVRSPGQRSLRSSVTDGGLRVCEITRDGAMQTALWHRRKTLLKSALRYWISRQQEEHRKREEKQEQEREEKRRQRKEVKKRLLGELANVPPRKSARLLTQAPRPTIALIPRKFRDPPERLPAAPKQLLTLSAKTLSRKPLRRTARLHRYHASQSARRRKLAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKSNHPQVGALSAGGGGGGGGVPSSLQSAEQKEPRSSSRQQVSLCHELHTLFRVMWSGRWSLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELESDAPRKHFLIPITQRKLSKQVLKVLNTIFHGQLLSQVTCLSCKRKSNTVEPFWDLSLEFPERYHRMEKPSAAACQRSCSLSEMLAKFTETEALEGCIYACNFCNRKRRKSSHKPLSLSEACKQLLICRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIQPYCSCSDAAAREAYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMNVCSVEEVCSTQAYILFYTQRSS

Target ORF information:

RefSeq Version	XM_009295758.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295758.2

ORF Insert Sequence:

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp49 ( XM_009295758.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294033.1 Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa26322
Clone ID Related Accession (Same CDS sequence)	XM_005168272.4 , XM_005168272.3 , NM_001044896.1 , XM_009295758.2 , XM_009295758.3
Accession Version	XM_009295758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 49 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295758.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294033.1
CDS	1378..3327
Translation	MDRCKHVVRFRLGHGHSILNPQMWRCVDCDTTESVWACLKCTHVACGRYMEEHSRSHYQQTQHPLAMDVRELDVFCFACGDYVLNDNVEGDLKLLRGALSTVRSPGQRSLRSSVTDGGLRVCEITRDGAMQTALWHRRKTLLKSALRYWISRQQEEHRKREEKQEQEREEKRRQRKEVKKRLLGELANVPPRKSARLLTQAPRPTIALIPRKFRDPPERLPAAPKQLLTLSAKTLSRKPLRRTARLHRYHASQSARRRKLAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKSNHPQVGALSAGGGGGGGGVPSSLQSAEQKEPRSSSRQQVSLCHELHTLFRVMWSGRWSLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELESDAPRKHFLIPITQRKLSKQVLKVLNTIFHGQLLSQVTCLSCKRKSNTVEPFWDLSLEFPERYHRMEKPSAAACQRSCSLSEMLAKFTETEALEGCIYACNFCNRKRRKSSHKPLSLSEACKQLLICRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIQPYCSCSDAAAREAYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMNVCSVEEVCSTQAYILFYTQRSS

Target ORF information:

RefSeq Version	XM_009295758.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 49 (usp49), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295758.3

ORF Insert Sequence:

ATGGATCGCT GTAAGCACGT GGTGCGGTTC CGCCTGGGCC ACGGCCACTC CATCCTCAAC 
CCGCAGATGT GGCGCTGCGT GGACTGCGAT ACCACCGAGT CCGTGTGGGC CTGCCTGAAG 
TGCACGCACG TAGCCTGCGG ACGCTACATG GAGGAGCACT CACGGAGCCA CTACCAGCAG 
ACTCAGCATC CGCTAGCCAT GGATGTACGC GAGCTGGATG TCTTCTGCTT TGCGTGCGGA 
GATTACGTGT TGAACGATAA CGTGGAGGGC GATCTCAAGC TCCTGCGGGG GGCGCTGTCC 
ACCGTCCGCA GCCCTGGCCA GCGTTCCCTG CGTTCGTCAG TGACGGATGG CGGATTGAGA 
GTGTGTGAGA TTACGCGAGA CGGCGCGATG CAGACGGCGT TATGGCACCG GCGGAAAACT 
CTTCTCAAAA GCGCACTTCG CTACTGGATT AGCCGGCAAC AGGAAGAGCA CAGGAAGCGC 
GAGGAAAAAC AAGAACAGGA GCGAGAGGAA AAACGACGCC AGCGTAAAGA AGTCAAAAAA 
AGACTGCTAG GAGAATTAGC CAATGTGCCG CCTAGAAAAA GTGCGCGATT GCTAACCCAG 
GCTCCGCGTC CAACAATCGC GCTAATACCA CGGAAGTTCC GCGACCCTCC AGAGCGACTT 
CCTGCAGCGC CCAAACAGTT ATTGACGCTA TCTGCAAAGA CTCTTTCGCG GAAACCGCTC 
CGCCGTACTG CACGCCTGCA CCGCTATCAT GCTTCACAAT CTGCGCGACG CAGGAAACTA 
GCGCCCGGAG TTACAGGACT GCGCAATCTC GGGAATACAT GCTACATGAA CTCCATCCTA 
CAGGTGCTCA GCCACTTGCA GAAGTTCCGG GAGTGTTTCC TGACGCTGGA CCTGTGCGAG 
ACGGAGGAGC TTCTGGCTAA AAGCAACCAC CCTCAGGTGG GGGCTCTGTC TGCGGGTGGC 
GGCGGCGGCG GCGGCGGTGT CCCATCCAGC CTGCAGTCAG CGGAGCAGAA GGAGCCGCGC 
TCCTCATCCC GCCAGCAGGT GTCGCTGTGC CACGAGCTGC ACACGCTCTT CAGGGTGATG 
TGGTCGGGCC GCTGGTCGCT GGTGTCGCCC TTCGCCATGC TGCACTCCGT CTGGAACCTG 
ATCCCTGCTT TTCGCGGGTA CGACCAGCAG GACGCGCAGG AGTTCCTGTG CGAGCTCCTG 
GATAAAGTGC AGCAGGAACT GGAGTCGGAC GCACCCCGCA AACACTTCCT GATCCCCATC 
ACACAGCGCA AACTCTCCAA ACAGGTCCTC AAAGTGCTCA ACACCATCTT CCACGGACAG 
CTGCTCAGCC AGGTGACGTG TCTGTCCTGC AAGCGGAAGT CCAACACAGT GGAGCCGTTC 
TGGGATTTGT CTCTCGAGTT CCCAGAGCGG TACCACCGCA TGGAGAAGCC CAGCGCCGCC 
GCCTGCCAGA GGAGCTGCTC GCTGTCCGAG ATGCTGGCCA AGTTCACGGA GACCGAAGCT 
CTGGAGGGCT GCATTTACGC CTGTAACTTC TGCAACAGAA AGCGGCGTAA ATCGTCCCAC 
AAGCCGCTGT CACTGTCAGA GGCCTGCAAG CAGCTGCTGA TCTGCAGATT ACCTCAGGTG 
CTGCGGCTAC ACCTCAAACG CTTCCGGTGG TCGGGCAGGA ACCACAGGGA GAAGATCGGT 
GTGCACGTGG CGTTTGATCA GGTGTTGAAC ATCCAGCCGT ACTGCAGCTG CTCAGACGCC 
GCCGCGAGAG AGGCCTACAC ATACGACCTG TCTGCGGTCG TGATGCATCA TGGGAAGGGC 
TTTGGCTCAG GGCACTACAC AGCCTACTGC TACAACACTG AAGGAGGCTT CTGGGTTCAC 
TGTAATGACT CTGAGATGAA CGTGTGCAGC GTGGAGGAGG TGTGCAGCAC TCAGGCCTAC 
ATCCTCTTCT ACACACAGCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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usp49 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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