LRRK2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRK2
Entrez Gene ID	559366
Full Name	leucine-rich repeat kinase 2
General protein information	Preferred Names leucine-rich repeat kinase 2 Names leucine-rich repeat serine/threonine-protein kinase 2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470500.1	XP_021326175.1	leucine-rich repeat serine/threonine-protein kinase 2 isoform X2
XM_009298197.3	XP_009296472.2	leucine-rich repeat serine/threonine-protein kinase 2 isoform X1
NM_001201456.2	NP_001188385.2	leucine-rich repeat serine/threonine-protein kinase 2
XM_009298197.2	XP_009296472.2	leucine-rich repeat serine/threonine-protein kinase 2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LRRK2	XP_003640388.2
Xenopus tropicalis (tropical clawed frog)	lrrk2	XP_002932250.2
	LRRK2	XP_002798616.1
Mus musculus (house mouse)	Lrrk2	NP_080006.3
Rattus norvegicus (Norway rat)	Lrrk2	NP_001178718.1
Danio rerio (zebrafish)	lrrk2	NP_001188385.1
Homo sapiens (human)	LRRK2	NP_940980.3
Pan troglodytes (chimpanzee)	LRRK2	XP_001168494.1
Canis lupus familiaris (dog)	LRRK2	XP_543734.3
Bos taurus (cattle)	LRRK2	NP_001193015.1

Gene Ontology
Bibliography

Related articles in PubMed

LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation.
Prabhudesai S, Bensabeur FZ, Abdullah R, Basak I, Baez S, Alves G, Holtzman NG, Larsen JP, M?ller SG
Journal of neuroscience research94(8)717-35(2016 08)

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Sarath Babu N, Murthy ChL, Kakara S, Sharma R, Brahmendra Swamy CV, Idris MM
Proteomics16(9)1407-20(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chronic exposure to low benzo[a]pyrene level causes neurodegenerative disease-like syndromes in zebrafish (Danio rerio).
Gao D, Wu M, Wang C, Wang Y, Zuo Z
Aquatic toxicology (Amsterdam, Netherlands)167200-8(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LRRK2 has multifaceted roles in zebrafish and that zebrafish represent a complementary model to further our understanding of this central protein
Title: LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation.

our similar early study and recent confirming experiments using the same reagents reported by Sheng et al. failed to reproduce the phenotype of the loss of dopaminergic neurons, although the mRNA of LRRK2 was molecularly disrupted
Title: Disruption of LRRK2 does not cause specific loss of dopaminergic neurons in zebrafish.

results demonstrate that zLRRK2 is an ortholog of hLRRK2 and that the deletion of WD40 domain of zLRRK2 provides a disease model for PD
Title: Deletion of the WD40 domain of LRRK2 in Zebrafish causes Parkinsonism-like loss of neurons and locomotive defect.

The following LRRK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53194	NM_001201456.2 Latest version!	Danio rerio leucine-rich repeat kinase 2 (lrrk2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa141331	XM_021470500.1 Latest version!	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa57801	XM_009298197.3 Latest version!	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
View Old Accession Versions >>
ODa57801	XM_009298197.2	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRK2 ( NM_001201456.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001188385.2 Danio rerio leucine-rich repeat kinase 2 (lrrk2), mRNA.

Price & Availability↑

CloneID	ODa53194
Clone ID Related Accession (Same CDS sequence)	NM_001201456.2
Accession Version	NM_001201456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat serine/threonine-protein kinase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU855815.10. On Sep 2, 2015 this sequence version replaced NM_001201456.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: HM143897.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGC GGAGGACTCT 
GTGCTGACGC TCTGGAGCAG TTCTCTTCCA CTGGAGGCTC GAGATCTGAT GCGACACTGT 
GAGCTCCGAG ACAAAACCAC CCGCAGAATG AGAGAAACGC TGCTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188385.2
CDS	148..7818
Translation	MLMCQHGGSESAFKFKFDVRNSEIMAEIEELSIRLKKLLVRLNLQDGKQLGTMVQIIEDLLFLSHTEHCVELFADQNVHVPVLLKCSESASIKVQQVGWSLLCRLMEICPNTLDNLARPVDYEFIDAHKQILKVLHEYHNKDAKMMMVALRALALMLKSGEIKMQVLDEEEWDVFYSILEAMKSFSDREEVQLQGCTALQPLLQTVSEYHLAEFIEKKDHEVVLNALGCFMDSENVVLEALKVLIPLADPASNVETLMSKTVKCHSLTCRAMNTWLDSEAIQEAGCCLLWKFTSKGYYDMLVLNGVHKVAVKACVSYPDNATLQTAALSCLSALAECIVQNGGLDEEWNEEDEEEQKVLVKKEAAQAEEMLIWREACYTAFERHAEDVKVQEAACWALNSLLLHCNTSNNVELEGRPPLHTLIMAAMLLHSSSVKVFQAASSTLRTLIQRHCRIRSPLLSNGIHFNIVELMRKHPNSSAVCESACKLIHTLFQGARASLDDGFLILSQILIALKTHNFLPEVQLEGLRASLVLLNPDRSLREHGVSVADPDMVDVSLQVLKNQCVLEGAHTVYLQALNRFISSESIQECGLGVLAALADSSGAVDLMCQQGAIDTVLHTLQMFPQERDIHYWGLSLLFHLISKKKLSRMMVPVLASVLVSSVRKHKEDSVMLLKGLQVVWKLLDTCSSAAVWLQKEAFEKEIFQILRENTADQRRDPLQGMSCLCLSKMVTDGEILYALLERACEDGDVDMAECLIQLGADVNKKTKSDSLLYQACDRGAPLALLELLVSSGVHEQHLRGALSVCVRRSDDPAVTLILRRLGLDHTNSALCLGSVRIGHMKASWISALLSERRSQSTNTHYSSKGQRLARQISQLQRKKGIVGVSRLSSDVSTSGYFTDEESDDSHISLEDSLVFMFDELESDGSDGPPHGFMLISDSLEVSRRPVWRRRSHSRRTSSEGYHGEADPSVPVQKHANSHNIRGQGFSESFSSPVVLDKDPVRLLDLSGNELNDLSCLTDLNSLKKLIENLHRLDLSGNNLSQFPSILCQSLRSLTRLDLQGNHLQCLPSELLSLPALHTLNVSRNCIGPLLQLEPGVCSPALRRLNLSFNQITVCPFQLRSATQRLEELSLEGNQISELSLPLCLAELKVLDVSKNQVKIVSDNFLAECLKMETFIASVNQISSLPHLPSKITTVKLSHNTFTRVPEIVINLPCLRSVDMRNNSVGVLPGPSVWLSVNLRELMFSHNLISALDLSGPVYKWARLEKLHLSFNRLTEIPPQIGMLEDLTSLDVSHNEGLRSFPDEMGKLVHLWDLPLDGLQLQLDLKHIGSKTKDIIRFLQQRLKKAVPYHRMKLMVLGGSGSGKSSLIQQLMRLRRSQWRSDPGVSIRDWPVRSKDKRNMMLNVWEFSGGEECSGIHPHFMSSRAVYLVLYDLSKGASEIHAIKPWLFNIKAVAGQCPVIVVGTHADLCEERHLQECLLKLQKELQSQPGFPAIRENHVLSACEESESLGRLRKAIYRELIGFKIQGQPVMGQLVPDCYVELEKRLLQERSCAPADFPVLRHSRLMEILQETQLQLEEGELPHAIHFLSEAGVLLHFDPVLQLKDLYFIDLQWFCRIISQTLSLKSSGPWDSTKGVVQRSTVEKFVEKSRCFPKDHMIQYFKLLEKFQIALPFDHDQLLIPSSLSDHRPVIELPHCENSEVIIRLYEMPYFPMGYWPRQISRLLEVSAFLLYGREKALKPNRIYWRKGIYLSWSAEAYCLVEALTLEENPASFIKITVPCSRKGRVLFGQVVDHIDSLLEEWFPGLLTTDIHGTGETLLKKWALYSFSDGQNCQKMLLEDLLSNTNADGLLVNPEDPSCTLPISQISPDLVLSDQPSSTILDPEQLEMELTAEYMLGDGGFGSVYKAVYKNEEVAVKIFNKHASALYVHRLVRQELAVLGRLCHPSLVGLLAAGCNPHILVMELAPYGSLDSLFERENGSLSRKLQHRIALHVADGLKYLHSSMIIYRDLKPHNVLLFNLKTDAEIVAKITDYGIAQYCCSMGVRSSEGTPGFRAPEVARGNVIYNVQADVYSFGLLLYDLLTYGERISDGMKFPSEFDEVAVQGKLPDPVKDYGCSPWPEIESLMRECMRENPQDRPTSAQVFDRLNSAEMLCLMRELNVVGFPGECFVVSNSGGAANGGKNPHVWIGGGSSSQKLGCVTAVDLETGGSLNQELDRSPILCMVIIRAADSCSDWLVAGSESGSLFIMDTINAKVLHRLKSVKDSVTSLYFHTELQHRCLKSYLLVGTADGTLVIYEDSALKVENGGPVKTLEVGDVNTPLMCLGPSSHPQERRSLWAACGTRIILFTVEFDVCRSIDTKPKPLFSLQARVSGEACISRLAVDKHVYVSKTGGHTVEVWDKKTERMVNLIDCMQLLGLSSTRKPKVHSEDQSRPMVPSLVVKALLVQHSGTLWIGTRAGHILLVEVSSCHLLQTINPHCHSIRCMGSILLDTLNRKNVILVLGRRQRIHLEQLKTQSAEDSVLTLWSSSLPLEARDLMRHCELRDKTTRRMRETLLN

Target ORF information:

RefSeq Version	NM_001201456.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeat kinase 2 (lrrk2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201456.2

ORF Insert Sequence:

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGC GGAGGACTCT 
GTGCTGACGC TCTGGAGCAG TTCTCTTCCA CTGGAGGCTC GAGATCTGAT GCGACACTGT 
GAGCTCCGAG ACAAAACCAC CCGCAGAATG AGAGAAACGC TGCTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK2 ( XM_021470500.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021326175.1 Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X3, mRNA.

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CloneID	ODa141331
Clone ID Related Accession (Same CDS sequence)	XM_021470500.1
Accession Version	XM_021470500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat serine/threonine-protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT AAAAACCCTC 
ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326175.1
CDS	119..6646
Translation	MLMCQHGGSESAFKFKFDVRNSEIMAEIEELSIRLKKLLVRLNLQDGKQLGTMVQIIEDLLFLSHTEHCVELFADQNVHVPVLLKCSESASIKVQQVGWSLLCRLMEICPNTLDNLARPVDYEFIDAHKQILKVLHEYHNKDAKMMMVALRALALMLKSGEIKMQVLDEEEWDVFYSILEAMKSFSDREEVQLQGCTALQPLLQTVSEYHLAEFIEKKDHEVVLNALGCFMDSENVVLEALKVLIPLADPASNVETLMSKTVKCHSLTCRAMNTWLDSEAIQEAGCCLLWKFTSKGYYDMLVLNGVHKVAVKACVSYPDNATLQTAALSCLSALAECIVQNGGLDEEWNEEDEEEQKVLVKKEAAQAEEMLIWREACYTAFERHAEDVKVQEAACWALNSLLLHCNTSNNVELEGRPPLHTLIMAAMLLHSSSVKVFQAASSTLRTLIQRHCRIRSPLLSNGIHFNIVELMRKHPNSSAVCESACKLIHTLFQGARASLDDGFLILSQILIALKTHNFLPEVQLEGLRASLVLLNPDRSLREHGVSVADPDMVDVSLQVLKNQCVLEGAHTVYLQALNRFISSESIQECGLGVLAALADSSGAVDLMCQQGAIDTVLHTLQMFPQERDIHYWGLSLLFHLISKKKLSRMMVPVLASVLVSSVRKHKEDSVMLLKGLQVVWKLLDTCSSAAVWLQKEAFEKEIFQILRENTADQRRDPLQGMSCLCLSKMVTDGEILYALLERACEDGDVDMAECLIQLGADVNKKTKSDSLLYQACDRGAPLALLELLVSSGVHEQHLRGALSVCVRRSDDPAVTLILRRLGLDHTNSALCLGSVRIGHMKASWISALLSERRSQSTNTHYSSKGQRLARQISQLQRKKGIVGVSRLSSDVSTSGYFTDEESDDSHISLEDSLVFMFDELESDGSDGPPHGFMLISDSLEVSRRPVWRRRSHSRRTSSEGYHGEADPSVPVQKHANSHNIRGQGFSESFSSPVVLDKDPVRLLDLSGNELNDLSCLTDLNSLKKLIENLHRLDLSGNNLSQFPSILCQSLRSLTRLDLQGNHLQCLPSELLSLPALHTLNVSRNCIGPLLQLEPGVCSPALRRLNLSFNQITVCPFQLRSATQRLEELSLEGNQISELSLPLCLAELKVLDVSKNQVKIVSDNFLAECLKMETFIASVNQISSLPHLPSKITTVKLSHNTFTRVPEIVINLPCLRSVDMRNNSVGVLPGPSVWLSVNLRELMFSHNLISALDLSGPVYKWARLEKLHLSFNRLTEIPPQIGMLEDLTSLDVSHNEGLRSFPDEMGKLVHLWDLPLDGLQLQLDLKHIGSKTKDIIRFLQQRLKKAVPYHRMKLMVLGGSGSGKSSLIQQLMRLRRSQWRSDPGVSIRDWPVRSKDKRNMMLNVWEFSGGEECSGIHPHFMSSRAVYLVLYDLSKGASEIHAIKPWLFNIKAVAGQCPVIVVGTHADLCEERHLQECLLKLQKELQSQPGFPAIRENHVLSACEESESLGRLRKAIYRELIGFKIQGQPVMGQLVPDCYVELEKRLLQERSCAPADFPVLRHSRLMEILQETQLQLEEGELPHAIHFLSEAGVLLHFDPVLQLKDLYFIDLQWFCRIISQTLSLKSSGPWDSTKGVVQRSTVEKFVEKSRCFPKDHMIQYFKLLEKFQIALPFDHDQLLIPSSLSDHRPVIELPHCENSEVIIRLYEMPYFPMGYWPRQISRLLEVSAFLLYGREKALKPNRIYWRKGIYLSWSAEAYCLVEALTLEENPASFIKITVPCSRKGRVLFGQVVDHIDSLLEEWFPGLLTTDIHGTGETLLKKWALYSFSDGQNCQKMLLEDLLSNTNADGLLVNPEDPSCTLPISQISPDLVLSDQPSSTILDPEQLEMELTAEYMLGDGGFGSVYKAVYKNEEVAVKIFNKHASALYVHRLVRQELAVLGRLCHPSLVGLLAAGCNPHILVMELAPYGSLDSLFERENGSLSRKLQHRIALHVADGLKYLHSSMIIYRDLKPHNVLLFNLKTDAEIVAKITDYGIAQYCCSMGVRSSEGTPGFRAPEVARGNVIYNVQADVYSFGLLLYDLLTYGERISDGMKFPSEFDEVAVQGKLPDPVKDYGCSPWPEIESLMRECMRENPQDRPTSAQASVLSCPIQAELQTEVKTLMCGSVVAAAARNWAV

Target ORF information:

RefSeq Version	XM_021470500.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470500.1

ORF Insert Sequence:

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT AAAAACCCTC 
ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK2 ( XM_009298197.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296472.2 Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.

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CloneID	ODa57801
Clone ID Related Accession (Same CDS sequence)	XM_009298197.3 , XM_009298197.2
Accession Version	XM_009298197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat serine/threonine-protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGA GGGTTTTTTG 
GTATCCACTT TAAGTCATCA AAACAGGACC TGGACAATCC ATCCTCATAT CCACAGACTT 
TTTCCATCCA CTTTTCTACT GATTCCGTGG CCAGCCATTG ATGAACTGTT TGCTGAACTT 
GATCTTTCAT GTGATTCACT TAAGGACTGT TGGTATCTTT TTTGGATTGC TATAAATACT 
GGATTCAATG AGAGACACTT ATCGAATGCT TATTTCTGTC TAATAGTGAT CATTAATGAG 
TTGACTGCTG ATCATTTCAT ATCCCCAGTG TGTAACAATA ATTATTCATA CCTGCTGTCA 
TATACATTTC TAAATACACA TGAATCTCTG AAAGTTGTTT TGTGGTCTCT CAATTTCTTT 
AATGTAGCTT CAAAAAAAAG TAGTTGTTAC ATATTTGGCG CCCGAACAAA AGACAAAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296472.2
CDS	119..8101
Translation	MLMCQHGGSESAFKFKFDVRNSEIMAEIEELSIRLKKLLVRLNLQDGKQLGTMVQIIEDLLFLSHTEHCVELFADQNVHVPVLLKCSESASIKVQQVGWSLLCRLMEICPNTLDNLARPVDYEFIDAHKQILKVLHEYHNKDAKMMMVALRALALMLKSGEIKMQVLDEEEWDVFYSILEAMKSFSDREEVQLQGCTALQPLLQTVSEYHLAEFIEKKDHEVVLNALGCFMDSENVVLEALKVLIPLADPASNVETLMSKTVKCHSLTCRAMNTWLDSEAIQEAGCCLLWKFTSKGYYDMLVLNGVHKVAVKACVSYPDNATLQTAALSCLSALAECIVQNGGLDEEWNEEDEEEQKVLVKKEAAQAEEMLIWREACYTAFERHAEDVKVQEAACWALNSLLLHCNTSNNVELEGRPPLHTLIMAAMLLHSSSVKVFQAASSTLRTLIQRHCRIRSPLLSNGIHFNIVELMRKHPNSSAVCESACKLIHTLFQGARASLDDGFLILSQILIALKTHNFLPEVQLEGLRASLVLLNPDRSLREHGVSVADPDMVDVSLQVLKNQCVLEGAHTVYLQALNRFISSESIQECGLGVLAALADSSGAVDLMCQQGAIDTVLHTLQMFPQERDIHYWGLSLLFHLISKKKLSRMMVPVLASVLVSSVRKHKEDSVMLLKGLQVVWKLLDTCSSAAVWLQKEAFEKEIFQILRENTADQRRDPLQGMSCLCLSKMVTDGEILYALLERACEDGDVDMAECLIQLGADVNKKTKSDSLLYQACDRGAPLALLELLVSSGVHEQHLRGALSVCVRRSDDPAVTLILRRLGLDHTNSALCLGSVRIGHMKASWISALLSERRSQSTNTHYSSKGQRLARQISQLQRKKGIVGVSRLSSDVSTSGYFTDEESDDSHISLEDSLVFMFDELESDGSDGPPHGFMLISDSLEVSRRPVWRRRSHSRRTSSEGYHGEADPSVPVQKHANSHNIRGQGFSESFSSPVVLDKDPVRLLDLSGNELNDLSCLTDLNSLKKLIENLHRLDLSGNNLSQFPSILCQSLRSLTRLDLQGNHLQCLPSELLSLPALHTLNVSRNCIGPLLQLEPGVCSPALRRLNLSFNQITVCPFQLRSATQRLEELSLEGNQISELSLPLCLAELKVLDVSKNQVKIVSDNFLAECLKMETFIASVNQISSLPHLPSKITTVKLSHNTFTRVPEIVINLPCLRSVDMRNNSVGVLPGPSVWLSVNLRELMFSHNLISALDLSGPVYKWARLEKLHLSFNRLTEIPPQIGMLEDLTSLDVSHNEGLRSFPDEMGKLVHLWDLPLDGLQLQLDLKHIGSKTKDIIRFLQQRLKKAVPYHRMKLMVLGGSGSGKSSLIQQLMRLRRSQWRSDPGVSIRDWPVRSKDKRNMMLNVWEFSGGEECSGIHPHFMSSRAVYLVLYDLSKGASEIHAIKPWLFNIKAVAGQCPVIVVGTHADLCEERHLQECLLKLQKELQSQPGFPAIRENHVLSACEESESLGRLRKAIYRELIGFKIQGQPVMGQLVPDCYVELEKRLLQERSCAPADFPVLRHSRLMEILQETQLQLEEGELPHAIHFLSEAGVLLHFDPVLQLKDLYFIDLQWFCRIISQTLSLKSSGPWDSTKGVVQRSTVEKFVEKSRCFPKDHMIQYFKLLEKFQIALPFDHDQLLIPSSLSDHRPVIELPHCENSEVIIRLYEMPYFPMGYWPRQISRLLEVSAFLLYGREKALKPNRIYWRKGIYLSWSAEAYCLVEALTLEENPASFIKITVPCSRKGRVLFGQVVDHIDSLLEEWFPGLLTTDIHGTGETLLKKWALYSFSDGQNCQKMLLEDLLSNTNADGLLVNPEDPSCTLPISQISPDLVLSDQPSSTILDPEQLEMELTAEYMLGDGGFGSVYKAVYKNEEVAVKIFNKHASALYVHRLVRQELAVLGRLCHPSLVGLLAAGCNPHILVMELAPYGSLDSLFERENGSLSRKLQHRIALHVADGLKYLHSSMIIYRDLKPHNVLLFNLKTDAEIVAKITDYGIAQYCCSMGVRSSEGTPGFRAPEVARGNVIYNVQADVYSFGLLLYDLLTYGERISDGMKFPSEFDEVAVQGKLPDPVKDYGCSPWPEIESLMRECMRENPQDRPTSAQVFDRLNSAEMLCLMRELNVVGFPGECFVVSNSGGAANGGKNPHVWIGGGSSSQKLGCVTAVDLETGGSLNQELDRSPILCMVIIRAADSCSDWLVAGSESGSLFIMDTINAKVLHRLKSVKDSVTSLYFHTELQHRCLKSYLLVGTADGTLVIYEDSALKVENGGPVKTLEVGDVNTPLMCLGPSSHPQERRSLWAACGTRIILFTVEFDVCRSIDTKPKPLFSLQARVSGEACISRLAVDKHVYVSKTGGHTVEVWDKKTERMVNLIDCMQLLGLSSTRKPKVHSEDQSRPMVPSLVVKALLVQHSGTLWIGTRAGHILLVEVSSCHLLQTINPHCHSIRCMGSILLDTLNRKNVILVLGRRQRIHLEQLKTQSEGFLVSTLSHQNRTWTIHPHIHRLFPSTFLLIPWPAIDELFAELDLSCDSLKDCWYLFWIAINTGFNERHLSNAYFCLIVIINELTADHFISPVCNNNYSYLLSYTFLNTHESLKVVLWSLNFFNVASKKSSCYIFGARTKDKI

Target ORF information:

RefSeq Version	XM_009298197.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298197.3

ORF Insert Sequence:

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGA GGGTTTTTTG 
GTATCCACTT TAAGTCATCA AAACAGGACC TGGACAATCC ATCCTCATAT CCACAGACTT 
TTTCCATCCA CTTTTCTACT GATTCCGTGG CCAGCCATTG ATGAACTGTT TGCTGAACTT 
GATCTTTCAT GTGATTCACT TAAGGACTGT TGGTATCTTT TTTGGATTGC TATAAATACT 
GGATTCAATG AGAGACACTT ATCGAATGCT TATTTCTGTC TAATAGTGAT CATTAATGAG 
TTGACTGCTG ATCATTTCAT ATCCCCAGTG TGTAACAATA ATTATTCATA CCTGCTGTCA 
TATACATTTC TAAATACACA TGAATCTCTG AAAGTTGTTT TGTGGTCTCT CAATTTCTTT 
AATGTAGCTT CAAAAAAAAG TAGTTGTTAC ATATTTGGCG CCCGAACAAA AGACAAAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRK2 ( XM_009298197.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009296472.2 Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.

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CloneID	ODa57801
Clone ID Related Accession (Same CDS sequence)	XM_009298197.3 , XM_009298197.2
Accession Version	XM_009298197.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat serine/threonine-protein kinase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298197.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGA GGGTTTTTTG 
GTATCCACTT TAAGTCATCA AAACAGGACC TGGACAATCC ATCCTCATAT CCACAGACTT 
TTTCCATCCA CTTTTCTACT GATTCCGTGG CCAGCCATTG ATGAACTGTT TGCTGAACTT 
GATCTTTCAT GTGATTCACT TAAGGACTGT TGGTATCTTT TTTGGATTGC TATAAATACT 
GGATTCAATG AGAGACACTT ATCGAATGCT TATTTCTGTC TAATAGTGAT CATTAATGAG 
TTGACTGCTG ATCATTTCAT ATCCCCAGTG TGTAACAATA ATTATTCATA CCTGCTGTCA 
TATACATTTC TAAATACACA TGAATCTCTG AAAGTTGTTT TGTGGTCTCT CAATTTCTTT 
AATGTAGCTT CAAAAAAAAG TAGTTGTTAC ATATTTGGCG CCCGAACAAA AGACAAAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296472.2
CDS	119..8101
Translation	MLMCQHGGSESAFKFKFDVRNSEIMAEIEELSIRLKKLLVRLNLQDGKQLGTMVQIIEDLLFLSHTEHCVELFADQNVHVPVLLKCSESASIKVQQVGWSLLCRLMEICPNTLDNLARPVDYEFIDAHKQILKVLHEYHNKDAKMMMVALRALALMLKSGEIKMQVLDEEEWDVFYSILEAMKSFSDREEVQLQGCTALQPLLQTVSEYHLAEFIEKKDHEVVLNALGCFMDSENVVLEALKVLIPLADPASNVETLMSKTVKCHSLTCRAMNTWLDSEAIQEAGCCLLWKFTSKGYYDMLVLNGVHKVAVKACVSYPDNATLQTAALSCLSALAECIVQNGGLDEEWNEEDEEEQKVLVKKEAAQAEEMLIWREACYTAFERHAEDVKVQEAACWALNSLLLHCNTSNNVELEGRPPLHTLIMAAMLLHSSSVKVFQAASSTLRTLIQRHCRIRSPLLSNGIHFNIVELMRKHPNSSAVCESACKLIHTLFQGARASLDDGFLILSQILIALKTHNFLPEVQLEGLRASLVLLNPDRSLREHGVSVADPDMVDVSLQVLKNQCVLEGAHTVYLQALNRFISSESIQECGLGVLAALADSSGAVDLMCQQGAIDTVLHTLQMFPQERDIHYWGLSLLFHLISKKKLSRMMVPVLASVLVSSVRKHKEDSVMLLKGLQVVWKLLDTCSSAAVWLQKEAFEKEIFQILRENTADQRRDPLQGMSCLCLSKMVTDGEILYALLERACEDGDVDMAECLIQLGADVNKKTKSDSLLYQACDRGAPLALLELLVSSGVHEQHLRGALSVCVRRSDDPAVTLILRRLGLDHTNSALCLGSVRIGHMKASWISALLSERRSQSTNTHYSSKGQRLARQISQLQRKKGIVGVSRLSSDVSTSGYFTDEESDDSHISLEDSLVFMFDELESDGSDGPPHGFMLISDSLEVSRRPVWRRRSHSRRTSSEGYHGEADPSVPVQKHANSHNIRGQGFSESFSSPVVLDKDPVRLLDLSGNELNDLSCLTDLNSLKKLIENLHRLDLSGNNLSQFPSILCQSLRSLTRLDLQGNHLQCLPSELLSLPALHTLNVSRNCIGPLLQLEPGVCSPALRRLNLSFNQITVCPFQLRSATQRLEELSLEGNQISELSLPLCLAELKVLDVSKNQVKIVSDNFLAECLKMETFIASVNQISSLPHLPSKITTVKLSHNTFTRVPEIVINLPCLRSVDMRNNSVGVLPGPSVWLSVNLRELMFSHNLISALDLSGPVYKWARLEKLHLSFNRLTEIPPQIGMLEDLTSLDVSHNEGLRSFPDEMGKLVHLWDLPLDGLQLQLDLKHIGSKTKDIIRFLQQRLKKAVPYHRMKLMVLGGSGSGKSSLIQQLMRLRRSQWRSDPGVSIRDWPVRSKDKRNMMLNVWEFSGGEECSGIHPHFMSSRAVYLVLYDLSKGASEIHAIKPWLFNIKAVAGQCPVIVVGTHADLCEERHLQECLLKLQKELQSQPGFPAIRENHVLSACEESESLGRLRKAIYRELIGFKIQGQPVMGQLVPDCYVELEKRLLQERSCAPADFPVLRHSRLMEILQETQLQLEEGELPHAIHFLSEAGVLLHFDPVLQLKDLYFIDLQWFCRIISQTLSLKSSGPWDSTKGVVQRSTVEKFVEKSRCFPKDHMIQYFKLLEKFQIALPFDHDQLLIPSSLSDHRPVIELPHCENSEVIIRLYEMPYFPMGYWPRQISRLLEVSAFLLYGREKALKPNRIYWRKGIYLSWSAEAYCLVEALTLEENPASFIKITVPCSRKGRVLFGQVVDHIDSLLEEWFPGLLTTDIHGTGETLLKKWALYSFSDGQNCQKMLLEDLLSNTNADGLLVNPEDPSCTLPISQISPDLVLSDQPSSTILDPEQLEMELTAEYMLGDGGFGSVYKAVYKNEEVAVKIFNKHASALYVHRLVRQELAVLGRLCHPSLVGLLAAGCNPHILVMELAPYGSLDSLFERENGSLSRKLQHRIALHVADGLKYLHSSMIIYRDLKPHNVLLFNLKTDAEIVAKITDYGIAQYCCSMGVRSSEGTPGFRAPEVARGNVIYNVQADVYSFGLLLYDLLTYGERISDGMKFPSEFDEVAVQGKLPDPVKDYGCSPWPEIESLMRECMRENPQDRPTSAQVFDRLNSAEMLCLMRELNVVGFPGECFVVSNSGGAANGGKNPHVWIGGGSSSQKLGCVTAVDLETGGSLNQELDRSPILCMVIIRAADSCSDWLVAGSESGSLFIMDTINAKVLHRLKSVKDSVTSLYFHTELQHRCLKSYLLVGTADGTLVIYEDSALKVENGGPVKTLEVGDVNTPLMCLGPSSHPQERRSLWAACGTRIILFTVEFDVCRSIDTKPKPLFSLQARVSGEACISRLAVDKHVYVSKTGGHTVEVWDKKTERMVNLIDCMQLLGLSSTRKPKVHSEDQSRPMVPSLVVKALLVQHSGTLWIGTRAGHILLVEVSSCHLLQTINPHCHSIRCMGSILLDTLNRKNVILVLGRRQRIHLEQLKTQSEGFLVSTLSHQNRTWTIHPHIHRLFPSTFLLIPWPAIDELFAELDLSCDSLKDCWYLFWIAINTGFNERHLSNAYFCLIVIINELTADHFISPVCNNNYSYLLSYTFLNTHESLKVVLWSLNFFNVASKKSSCYIFGARTKDKI

Target ORF information:

RefSeq Version	XM_009298197.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeat kinase 2 (lrrk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298197.2

ORF Insert Sequence:

ATGTTAATGT GTCAACATGG AGGATCAGAA AGTGCCTTTA AGTTCAAGTT TGATGTCAGA 
AACTCGGAGA TCATGGCAGA AATAGAGGAG TTGTCCATCA GACTGAAGAA GCTACTGGTG 
AGGTTGAATC TTCAGGATGG GAAGCAGCTG GGGACAATGG TGCAGATCAT AGAGGATCTC 
CTGTTCCTGT CCCATACTGA GCACTGTGTT GAGCTGTTTG CGGATCAAAA TGTCCATGTG 
CCTGTGTTGC TGAAATGCAG CGAAAGCGCC AGCATCAAAG TTCAGCAGGT TGGCTGGTCT 
CTGCTGTGTC GGCTGATGGA AATCTGTCCC AACACTCTGG ATAATCTGGC CAGACCGGTG 
GACTATGAAT TTATAGATGC ACACAAGCAG ATTCTGAAGG TGCTTCACGA GTACCATAAT 
AAAGACGCCA AGATGATGAT GGTGGCCCTG AGAGCTCTGG CTCTGATGCT GAAGTCAGGT 
GAGATCAAGA TGCAGGTTCT GGATGAGGAG GAATGGGATG TGTTTTACTC GATTCTGGAG 
GCCATGAAGT CATTCAGTGA CAGAGAAGAA GTTCAGCTAC AGGGATGTAC AGCTTTACAA 
CCACTGCTGC AGACAGTTTC TGAGTATCAC CTGGCGGAGT TCATCGAGAA GAAGGACCAT 
GAGGTGGTTT TGAACGCGCT CGGATGCTTT ATGGACAGTG AAAATGTGGT ACTTGAAGCT 
CTGAAGGTCC TCATACCACT GGCTGATCCA GCCAGCAATG TTGAGACCCT GATGTCCAAA 
ACTGTGAAAT GCCATAGTCT AACATGTCGG GCTATGAACA CGTGGCTGGA CTCTGAGGCG 
ATACAGGAGG CTGGTTGCTG TTTGCTATGG AAATTTACAT CAAAGGGTTA CTATGATATG 
CTGGTGCTGA ACGGTGTCCA TAAAGTGGCC GTGAAGGCAT GCGTCTCTTA TCCTGACAAT 
GCCACATTGC AGACCGCAGC TCTTTCCTGT CTGAGTGCCC TTGCGGAGTG TATTGTACAA 
AATGGAGGAC TGGATGAAGA GTGGAATGAG GAGGACGAGG AGGAACAGAA AGTTCTGGTA 
AAGAAAGAAG CCGCTCAGGC GGAGGAGATG CTAATCTGGA GAGAAGCCTG TTATACAGCT 
TTTGAAAGAC ATGCTGAAGA CGTCAAAGTA CAGGAAGCTG CATGCTGGGC TTTAAATAGT 
TTGCTTCTCC ATTGCAACAC ATCCAACAAT GTGGAACTGG AAGGAAGGCC TCCTCTTCAT 
ACGCTCATCA TGGCTGCGAT GCTTTTGCAC TCATCCAGCG TGAAGGTTTT CCAAGCTGCT 
TCCTCTACAC TGCGCACGCT AATACAGCGC CACTGCAGGA TAAGATCTCC ACTACTCTCA 
AATGGCATTC ATTTCAACAT CGTGGAGCTG ATGCGGAAAC ACCCTAACTC CAGCGCTGTG 
TGTGAAAGTG CCTGCAAACT AATTCACACA CTCTTCCAGG GAGCGAGGGC CAGTCTGGAC 
GACGGGTTCC TGATTCTCAG TCAGATCCTC ATCGCTCTGA AGACTCACAA CTTCCTTCCT 
GAAGTTCAGC TGGAGGGTCT GCGAGCAAGT TTAGTTCTTC TTAATCCAGA CAGGAGTTTG 
AGAGAGCATG GCGTCTCGGT GGCAGATCCT GATATGGTGG ACGTTTCTCT ACAGGTGTTG 
AAGAACCAGT GTGTCCTAGA GGGAGCTCAC ACTGTCTATC TGCAAGCACT TAATAGGTTT 
ATCAGCAGCG AGTCGATTCA GGAGTGTGGT CTCGGCGTCC TGGCAGCGCT AGCCGACAGT 
TCGGGAGCTG TAGATCTGAT GTGCCAGCAG GGGGCGATAG ACACCGTTCT GCACACGCTA 
CAGATGTTTC CACAGGAGCG CGATATTCAC TACTGGGGTC TGAGTCTCCT CTTCCACCTG 
ATCTCTAAGA AGAAACTGTC TCGTATGATG GTTCCTGTGC TGGCGTCTGT GTTGGTCAGC 
TCTGTGCGGA AACACAAAGA GGACTCAGTC ATGCTGCTAA AGGGTCTACA GGTGGTGTGG 
AAGTTGTTGG ACACCTGCTC GAGTGCTGCT GTGTGGCTGC AGAAAGAAGC GTTCGAGAAG 
GAGATCTTCC AGATCCTGCG GGAGAACACG GCCGATCAGC GCAGAGATCC GCTGCAGGGA 
ATGAGCTGCC TGTGCTTGTC TAAGATGGTG ACGGATGGAG AGATCCTGTA TGCTCTGCTG 
GAGAGAGCGT GTGAGGATGG AGACGTGGAC ATGGCAGAGT GTCTGATTCA GCTGGGCGCC 
GACGTCAACA AGAAAACCAA GAGCGATTCA CTCCTATATC AGGCGTGTGA TCGTGGGGCT 
CCCCTGGCTC TGCTGGAGCT TTTGGTTTCT TCAGGCGTTC ATGAGCAGCA TCTGCGTGGA 
GCTCTGAGTG TGTGCGTGCG GAGGTCGGAT GATCCTGCTG TCACTCTGAT CCTCCGCCGT 
CTGGGACTTG ACCACACCAA CAGCGCCCTG TGTCTCGGCA GCGTCCGCAT CGGACACATG 
AAGGCCTCGT GGATCAGCGC TCTTCTGTCT GAGCGCAGGA GTCAATCTAC AAACACGCAC 
TACAGCAGTA AAGGTCAGCG TTTGGCCAGA CAGATCTCGC AGCTCCAGAG GAAGAAGGGG 
ATTGTGGGAG TTAGCAGATT ATCGAGTGAT GTCAGCACAT CAGGATATTT CACCGATGAG 
GAAAGTGACG ACTCGCATAT TTCCTTAGAG GACAGCTTGG TCTTTATGTT CGATGAGTTG 
GAGAGCGACG GCAGTGACGG CCCTCCACAT GGATTCATGC TAATCAGTGA TTCTCTGGAA 
GTTAGCAGGA GACCCGTCTG GAGGCGTCGC AGCCACAGCC GACGTACCAG TTCAGAGGGC 
TACCATGGAG AGGCGGATCC AAGTGTCCCT GTGCAGAAGC ACGCCAACTC CCACAACATC 
AGAGGTCAGG GTTTCTCTGA GAGTTTCTCC AGTCCTGTGG TCCTGGATAA AGACCCGGTG 
CGTCTGCTGG ATCTGTCTGG GAATGAGCTG AACGATCTCT CCTGTCTGAC GGATCTGAAC 
TCTCTCAAGA AACTGATTGA AAACCTTCAT CGGCTCGATT TGAGCGGCAA CAATCTGTCA 
CAGTTTCCCA GCATTCTCTG TCAGAGTTTG CGCAGTCTGA CTCGTCTGGA TCTGCAGGGA 
AACCATCTGC AGTGTTTGCC ATCTGAACTG CTCAGTTTAC CAGCGCTCCA CACACTGAAC 
GTGTCCCGCA ACTGCATCGG TCCGCTCCTG CAGCTGGAGC CCGGCGTCTG CAGTCCAGCC 
CTGCGGCGCC TCAACCTGTC CTTCAACCAG ATCACCGTCT GCCCATTCCA GCTGCGCAGC 
GCCACCCAGA GGCTGGAGGA GCTCTCACTG GAGGGAAATC AGATCTCCGA ACTCTCCCTC 
CCGCTCTGTC TGGCTGAGCT GAAGGTTTTA GACGTCAGTA AGAATCAGGT TAAAATAGTT 
TCAGACAACT TCCTTGCGGA ATGCCTCAAG ATGGAAACTT TCATCGCTTC TGTTAACCAG 
ATCAGTTCAC TGCCACACCT TCCCTCCAAA ATAACCACCG TGAAATTATC ACACAACACT 
TTCACCAGGG TTCCTGAAAT AGTCATCAAC TTGCCATGCT TGCGTTCGGT GGACATGAGG 
AATAACAGTG TTGGCGTTCT GCCGGGTCCA TCTGTCTGGC TGAGTGTGAA CCTCAGAGAG 
CTGATGTTCA GCCATAATCT CATCTCCGCA CTCGACCTCA GCGGCCCTGT GTACAAGTGG 
GCCCGACTGG AGAAACTGCA CTTAAGCTTC AACAGACTCA CTGAGATTCC TCCTCAGATC 
GGGATGCTGG AGGACCTGAC GTCTCTGGAT GTGAGCCATA ATGAGGGTCT GCGCTCGTTC 
CCGGATGAGA TGGGGAAGCT GGTGCATCTG TGGGATCTGC CTCTGGATGG CCTGCAGCTC 
CAGCTGGACC TCAAACACAT CGGGAGCAAA ACCAAAGACA TCATCAGGTT CCTGCAGCAG 
CGTCTGAAGA AGGCCGTCCC GTATCACCGC ATGAAGCTGA TGGTGTTGGG GGGCTCCGGC 
AGCGGAAAGA GCTCTTTAAT TCAGCAGCTG ATGAGACTCA GGCGCTCACA ATGGAGATCA 
GATCCAGGCG TCAGCATTCG GGACTGGCCC GTCAGGAGCA AAGACAAGAG GAACATGATG 
CTGAACGTCT GGGAGTTCTC AGGTGGTGAA GAGTGCAGTG GGATTCACCC TCACTTCATG 
AGCTCCAGAG CTGTCTATCT AGTGCTCTAC GACCTCAGTA AAGGAGCCAG CGAGATCCAC 
GCCATCAAAC CCTGGCTCTT TAACATTAAG GCTGTAGCGG GTCAATGTCC AGTGATTGTG 
GTGGGAACTC ATGCAGATTT GTGTGAAGAG CGCCACCTAC AGGAGTGTTT GCTGAAGCTG 
CAGAAAGAGC TTCAGTCTCA GCCCGGGTTC CCGGCGATCA GAGAAAACCA CGTGTTGAGT 
GCCTGCGAGG AGTCCGAGTC ACTCGGCCGG CTACGCAAAG CCATCTACAG AGAACTCATC 
GGGTTCAAGA TCCAGGGCCA GCCGGTGATG GGTCAGCTAG TTCCAGACTG TTATGTGGAG 
CTGGAGAAGA GACTCCTGCA GGAGAGATCA TGCGCTCCGG CTGATTTCCC AGTGCTCAGA 
CACAGCAGAC TGATGGAGAT CCTGCAGGAG ACGCAGTTAC AGCTGGAGGA GGGGGAGTTA 
CCTCACGCTA TACACTTCCT CAGCGAAGCC GGTGTCTTGC TGCACTTTGA TCCAGTTCTT 
CAGCTGAAGG ATCTGTACTT CATTGACCTA CAGTGGTTTT GTAGGATCAT CTCACAGACG 
CTGTCTCTGA AAAGCAGCGG TCCATGGGAC AGCACGAAAG GAGTGGTGCA GCGCTCCACC 
GTGGAGAAAT TTGTGGAGAA AAGCCGCTGT TTCCCAAAGG ATCACATGAT TCAATACTTC 
AAACTGTTGG AGAAGTTTCA GATAGCCCTG CCATTTGACC ATGACCAGCT ACTCATTCCT 
AGCAGTCTGT CAGACCACAG GCCAGTGATT GAGCTTCCTC ACTGTGAGAA TTCAGAAGTG 
ATCATTCGAC TATATGAGAT GCCATATTTT CCGATGGGCT ACTGGCCCAG ACAGATCAGC 
CGTCTGCTGG AAGTGTCTGC ATTCCTGCTC TATGGCAGAG AAAAAGCATT AAAACCAAAC 
CGGATCTACT GGAGGAAGGG CATCTATTTG AGCTGGTCTG CTGAAGCTTA CTGTCTGGTT 
GAGGCTTTGA CTTTAGAAGA AAACCCAGCT AGTTTCATCA AGATCACCGT TCCATGCTCT 
CGCAAAGGCC GTGTGTTGTT TGGGCAGGTG GTGGACCACA TTGACTCTCT GCTGGAGGAA 
TGGTTTCCAG GCCTTCTCAC CACCGACATT CATGGCACTG GAGAGACGCT GCTGAAGAAA 
TGGGCTCTGT ACAGCTTCAG CGATGGACAA AACTGCCAGA AGATGCTACT GGAAGACCTG 
CTCTCCAACA CCAATGCAGA TGGTCTACTG GTGAACCCCG AGGACCCCAG CTGTACTTTA 
CCCATCTCTC AGATTTCTCC AGACCTGGTA CTGAGTGACC AGCCATCCAG CACCATACTG 
GACCCAGAGC AGCTGGAGAT GGAGCTCACT GCAGAGTATA TGTTGGGGGA TGGAGGTTTT 
GGCTCGGTTT ATAAAGCTGT CTACAAGAAT GAAGAAGTTG CGGTCAAGAT TTTTAATAAA 
CATGCGTCTG CGCTGTACGT TCACCGGCTG GTACGACAGG AACTGGCAGT ACTCGGCAGA 
CTGTGTCATC CTAGTTTGGT GGGTCTTTTG GCTGCTGGTT GTAACCCACA TATACTTGTG 
ATGGAGTTGG CTCCGTATGG CTCTCTGGAC TCGCTGTTTG AGCGTGAAAA TGGCAGCTTA 
AGTCGCAAAT TACAGCACAG GATTGCACTA CATGTGGCTG ATGGTCTCAA ATATTTGCAC 
TCATCTATGA TCATCTACCG AGACCTGAAG CCGCACAACG TTCTGCTGTT CAACCTGAAG 
ACGGACGCTG AGATCGTCGC TAAAATCACA GACTATGGCA TCGCGCAGTA CTGCTGCAGC 
ATGGGCGTCC GCAGCTCTGA AGGAACACCA GGTTTTCGAG CCCCGGAGGT GGCTCGAGGA 
AACGTCATCT ACAACGTCCA GGCGGATGTT TATTCGTTCG GTCTGCTGCT GTATGATCTG 
TTAACATACG GCGAGCGCAT CTCAGATGGC ATGAAGTTTC CCAGTGAGTT CGATGAAGTG 
GCCGTGCAGG GCAAACTACC AGATCCAGTG AAGGATTACG GCTGTTCTCC ATGGCCTGAG 
ATTGAGTCTC TCATGAGGGA ATGTATGAGA GAAAACCCCC AGGACAGGCC GACGTCTGCT 
CAGGTGTTTG ATCGTCTGAA CTCTGCAGAG ATGTTGTGTC TGATGCGAGA GCTGAATGTT 
GTGGGTTTTC CAGGCGAGTG TTTTGTCGTG TCCAATTCAG GCGGAGCTGC AAACGGAGGT 
AAAAACCCTC ATGTGTGGAT CGGTGGTGGC AGCAGCAGCC AGAAACTGGG CTGTGTGACT 
GCGGTGGATC TGGAGACCGG CGGGAGTTTG AACCAGGAGC TGGACCGCAG CCCTATCCTC 
TGCATGGTGA TCATCAGAGC TGCAGACTCG TGCAGTGACT GGCTAGTGGC TGGTTCAGAG 
TCCGGTTCGC TCTTTATCAT GGACACCATT AATGCGAAAG TCCTGCACCG TCTGAAGAGT 
GTGAAGGACT CTGTGACGTC TCTGTACTTT CACACCGAGC TCCAGCACAG ATGTCTGAAG 
AGTTATCTTT TGGTGGGCAC AGCAGACGGG ACACTGGTCA TTTATGAAGA TTCAGCCCTG 
AAGGTGGAGA ACGGTGGTCC AGTGAAGACG CTGGAGGTTG GAGATGTGAA CACTCCGCTG 
ATGTGTCTCG GTCCATCCAG TCATCCTCAG GAGCGCAGAT CGCTGTGGGC CGCCTGTGGG 
ACCAGGATCA TCTTATTCAC TGTTGAATTT GACGTCTGTA GAAGCATTGA CACCAAACCC 
AAACCGCTCT TCTCACTGCA GGCTCGGGTC AGCGGGGAGG CTTGTATATC ACGGTTAGCG 
GTGGACAAAC ACGTGTATGT GAGTAAAACC GGAGGCCACA CTGTTGAGGT CTGGGACAAG 
AAGACTGAGA GGATGGTGAA CCTCATCGAC TGCATGCAGT TACTCGGATT AAGTTCCACC 
AGAAAGCCCA AAGTTCATTC AGAGGACCAG TCTAGACCGA TGGTGCCGTC GTTGGTTGTG 
AAAGCCTTAT TGGTCCAGCA CAGTGGGACT CTGTGGATCG GGACGAGAGC GGGACACATT 
TTGTTGGTGG AGGTTTCCAG TTGTCATCTT TTACAGACCA TCAACCCTCA CTGCCACTCC 
ATACGCTGCA TGGGCTCCAT ACTTCTAGAC ACGCTAAACC GAAAGAATGT CATTCTAGTA 
TTGGGTAGAC GGCAGCGCAT ACATCTAGAG CAGCTCAAAA CACAATCAGA GGGTTTTTTG 
GTATCCACTT TAAGTCATCA AAACAGGACC TGGACAATCC ATCCTCATAT CCACAGACTT 
TTTCCATCCA CTTTTCTACT GATTCCGTGG CCAGCCATTG ATGAACTGTT TGCTGAACTT 
GATCTTTCAT GTGATTCACT TAAGGACTGT TGGTATCTTT TTTGGATTGC TATAAATACT 
GGATTCAATG AGAGACACTT ATCGAATGCT TATTTCTGTC TAATAGTGAT CATTAATGAG 
TTGACTGCTG ATCATTTCAT ATCCCCAGTG TGTAACAATA ATTATTCATA CCTGCTGTCA 
TATACATTTC TAAATACACA TGAATCTCTG AAAGTTGTTT TGTGGTCTCT CAATTTCTTT 
AATGTAGCTT CAAAAAAAAG TAGTTGTTAC ATATTTGGCG CCCGAACAAA AGACAAAATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation.
Journal of neuroscience research94(8)717-35(2016 08)
Prabhudesai S,Bensabeur FZ,Abdullah R,Basak I,Baez S,Alves G,Holtzman NG,Larsen JP,M?ller SG

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced Parkinson's disease in zebrafish.
Proteomics16(9)1407-20(2016 05)
Sarath Babu N,Murthy ChL,Kakara S,Sharma R,Brahmendra Swamy CV,Idris MM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chronic exposure to low benzo[a]pyrene level causes neurodegenerative disease-like syndromes in zebrafish (Danio rerio).
Aquatic toxicology (Amsterdam, Netherlands)167200-8(2015 Oct)
Gao D,Wu M,Wang C,Wang Y,Zuo Z

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LRRK2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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