ralgapb gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009302762.3	XP_009301037.1	ral GTPase-activating protein subunit beta isoform X2
XM_009302765.2	XP_009301040.1	ral GTPase-activating protein subunit beta isoform X3
XM_009302766.3	XP_009301041.1	ral GTPase-activating protein subunit beta isoform X7
XM_009302765.3	XP_009301040.1	ral GTPase-activating protein subunit beta isoform X8
XM_009302763.2	XP_009301038.1	ral GTPase-activating protein subunit beta isoform X2
XM_009302762.2	XP_009301037.1	ral GTPase-activating protein subunit beta isoform X1
XM_009302766.2	XP_009301041.1	ral GTPase-activating protein subunit beta isoform X4
XM_021477330.1	XP_021333005.1	ral GTPase-activating protein subunit beta isoform X4
XM_021477331.1	XP_021333006.1	ral GTPase-activating protein subunit beta isoform X5
XM_021477332.1	XP_021333007.1	ral GTPase-activating protein subunit beta isoform X6
XM_021477328.1	XP_021333003.1	ral GTPase-activating protein subunit beta isoform X1
XM_009302763.3	XP_009301038.1	ral GTPase-activating protein subunit beta isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143153	XM_021477332.1 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa143150	XM_021477328.1 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa143151	XM_021477330.1 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa143152	XM_021477331.1 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa43042	XM_009302762.3 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43043	XM_009302763.3 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43045	XM_009302765.3 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa43046	XM_009302766.3 Latest version!	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa43045	XM_009302765.2	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa43042	XM_009302762.2	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43046	XM_009302766.2	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa43043	XM_009302763.2	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ralgapb ( XM_021477332.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333007.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa143153
Clone ID Related Accession (Same CDS sequence)	XM_021477332.1
Accession Version	XM_021477332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCGC CCCCAACAAC AACCAACACA ACACCGCCTC ACAACCGCAG GCACCGCCCC 
ACTGCTGTTG CCAAGACAAC CAGCAAATCC TCAACGACTG GGCAGACCAG AGTATCGCAT 
CAGCCTTCGT CCACATCTCC TCTCTCCAGC CCAAACCAGA CGAGCTCCGA GCCCCGTCCG 
CTCCCGGCTC CGACCCGGCC CAAAGTCAAC AGCGTGTTAA ATCTGTTCGG CCAGTGGCTG 
TTTGACGCTG CGCTGGTCCA CTGTAAACTG CACTCCGGCC TGAGCAGAGA CAGCAGCATG 
ACTGCAACAT TTTTCCAAAT TCTTCTTTCT TATAAATCTT CACTGGCCAC TCAAGCTGGA 
GTTGAACTGA GGCGTAAGGG CTCTCAGATG TCCACAGACA CCATGGTCTC CAACCCCATG 
TTTGACGCCA ATGAATTTCC TGATAATTAT GAGTCGGGTC GAGCTGAAGC ATGCGGGACG 
CTCTGCAGGA TCTTCTGCAG TAAGAAAACA GGAGAGGAGA TTCTTCCCGT CTACCTGTCC 
AGGTTCTACA TGGTGCTGAT CCAGGGTCTT CAGATTTCAG ACTTCATCTG CAGGCCGGTC 
TTAGCCAGCA TTATCCTGAA CTCTTCTGCT CTGTTCTGCT CCGATCTCAA GGGAATCAAT 
GTAGTGGTTC CCTACTTCAT ATCTGCACTG GAGACCATCC TGCCGGACCG AGAGCTGTCG 
AAGTTTAAGT CTTATGTGAA TCCTACTGAG CTGAGAAGAG CCTCCATCCA CATCCTGCTG 
TCCATGCTTC CGCTTCCTCA CCACTTCGGC AACGTCAAAT CAGAGGTTCT GTTGGAAGGG 
AAATTCAGCA ATGATGACAA CTCTCTCCAC GACAAGGCGG TCACCTTCCT CTCCCTGAAG 
CTGCGATTGG TCAATATTCT GATCGGCGCC CTGCAAACAG AGACAGACCC GATCAATACA 
CAGATGATAC TGGCGGCGAT GCTGAATATC GTGCAGGATT CTGCTCTGCT GGAGTCTGTT 
GGTGCTCAGG CTGAAGTGGG CAGTGTGGAT GGCAGCCATT CTCTGGCTAA GAGTCACAGT 
CGTAATAACA GTGGCATCAG CAATGCTAGC GCAGGCAGTT CAGAAGCCAC CACTCCAGAC 
AGCGAACGCC CGGCACAGGC CCTGCTGAGA GACTACGCTC TCCACACAGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333007.1
CDS	146..3553
Translation	MPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTATFFQILLSYKSSLATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_021477332.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477332.1

ORF Insert Sequence:

ATGCCACCGC CCCCAACAAC AACCAACACA ACACCGCCTC ACAACCGCAG GCACCGCCCC 
ACTGCTGTTG CCAAGACAAC CAGCAAATCC TCAACGACTG GGCAGACCAG AGTATCGCAT 
CAGCCTTCGT CCACATCTCC TCTCTCCAGC CCAAACCAGA CGAGCTCCGA GCCCCGTCCG 
CTCCCGGCTC CGACCCGGCC CAAAGTCAAC AGCGTGTTAA ATCTGTTCGG CCAGTGGCTG 
TTTGACGCTG CGCTGGTCCA CTGTAAACTG CACTCCGGCC TGAGCAGAGA CAGCAGCATG 
ACTGCAACAT TTTTCCAAAT TCTTCTTTCT TATAAATCTT CACTGGCCAC TCAAGCTGGA 
GTTGAACTGA GGCGTAAGGG CTCTCAGATG TCCACAGACA CCATGGTCTC CAACCCCATG 
TTTGACGCCA ATGAATTTCC TGATAATTAT GAGTCGGGTC GAGCTGAAGC ATGCGGGACG 
CTCTGCAGGA TCTTCTGCAG TAAGAAAACA GGAGAGGAGA TTCTTCCCGT CTACCTGTCC 
AGGTTCTACA TGGTGCTGAT CCAGGGTCTT CAGATTTCAG ACTTCATCTG CAGGCCGGTC 
TTAGCCAGCA TTATCCTGAA CTCTTCTGCT CTGTTCTGCT CCGATCTCAA GGGAATCAAT 
GTAGTGGTTC CCTACTTCAT ATCTGCACTG GAGACCATCC TGCCGGACCG AGAGCTGTCG 
AAGTTTAAGT CTTATGTGAA TCCTACTGAG CTGAGAAGAG CCTCCATCCA CATCCTGCTG 
TCCATGCTTC CGCTTCCTCA CCACTTCGGC AACGTCAAAT CAGAGGTTCT GTTGGAAGGG 
AAATTCAGCA ATGATGACAA CTCTCTCCAC GACAAGGCGG TCACCTTCCT CTCCCTGAAG 
CTGCGATTGG TCAATATTCT GATCGGCGCC CTGCAAACAG AGACAGACCC GATCAATACA 
CAGATGATAC TGGCGGCGAT GCTGAATATC GTGCAGGATT CTGCTCTGCT GGAGTCTGTT 
GGTGCTCAGG CTGAAGTGGG CAGTGTGGAT GGCAGCCATT CTCTGGCTAA GAGTCACAGT 
CGTAATAACA GTGGCATCAG CAATGCTAGC GCAGGCAGTT CAGAAGCCAC CACTCCAGAC 
AGCGAACGCC CGGCACAGGC CCTGCTGAGA GACTACGCTC TCCACACAGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_021477328.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333003.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143150
Clone ID Related Accession (Same CDS sequence)	XM_021477328.1
Accession Version	XM_021477328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCAACATTT 
TTCCAAATTC TTCTTTCTTA TAAATCTTCA CTGGCCACTC AAGCTGGAGT TGAACTGAGG 
CGTAAGGGCT CTCAGATGTC CACAGACACC ATGGTCTCCA ACCCCATGTT TGACGCCAAT 
GAATTTCCTG ATAATTATGA GTCGGGTCGA GCTGAAGCAT GCGGGACGCT CTGCAGGATC 
TTCTGCAGTA AGAAAACAGG AGAGGAGATT CTTCCCGTCT ACCTGTCCAG GTTCTACATG 
GTGCTGATCC AGGGTCTTCA GATTTCAGAC TTCATCTGCA GGCCGGTCTT AGCCAGCATT 
ATCCTGAACT CTTCTGCTCT GTTCTGCTCC GATCTCAAGG GAATCAATGT AGTGGTTCCC 
TACTTCATAT CTGCACTGGA GACCATCCTG CCGGACCGAG AGCTGTCGAA GTTTAAGTCT 
TATGTGAATC CTACTGAGCT GAGAAGAGCC TCCATCCACA TCCTGCTGTC CATGCTTCCG 
CTTCCTCACC ACTTCGGCAA CGTCAAATCA GAGGTTCTGT TGGAAGGGAA ATTCAGCAAT 
GATGACAACT CTCTCCACGA CAAGGCGGTC ACCTTCCTCT CCCTGAAGCT GCGATTGGTC 
AATATTCTGA TCGGCGCCCT GCAAACAGAG ACAGACCCGA TCAATACACA GATGATACTG 
GCGGCGATGC TGAATATCGT GCAGGATTCT GCTCTGCTGG AGTCTGTTGG TGCTCAGGCT 
GAAGTGGGCA GTGTGGATGG CAGCCATTCT CTGGCTAAGA GTCACAGTCG TAATAACAGT 
GGCATCAGCA ATGCTAGCGC AGGCAGTTCA GAAGCCACCA CTCCAGACAG CGAACGCCCG 
GCACAGGCCC TGCTGAGAGA CTACGCTCTC CACACAGACA CTGCGGCCGG GCTGTTGATC 
CGCAGCATTC ACCTGCTCAC CCAGAGACTC AACTCTCAGT GGAGACTCGA CATGAGCATC 
TCTCTGGCGG CGCTCGAGCT GCTCGCGGGA CTCGCCAAGG TGAAGGTGAG CGTGGAGTCG 
TCTGACCGCA AGCGTGCCGT CAGCTCGGTC TGCAGTTATA TTGTGTATCA ATGCAGTCGT 
CCAGCTCCGC TTCATTCCAG AGATCTGCAC TCCATGATCG TCGCTGCTTT CCACTGTCTG 
TGTGTGTGGC TTACTGAACA CCCCGACATG CTCAATGAGA AGGATTGTCT GATTGAAGTG 
TTGGAGATCG TGGAGCTCGG CATCTCCGGC AGCAAGTCCA AAACAGGCGA ACAGGAAGTG 
AAGTATAAAG GAGACAAAGA GCACAACCCT GCATCTATGA GGGTCAAAGA TGCCGCAGAG 
GCCACGCTAT CATGTATAAT GCAGGTTTTG GGCGCGTTCC CATCTCCCAG CGGTCCCGCA 
TCGCCCTGCA GTCTGCTGAA CGAGGACACG CTCATCAAAT ACTCCAGACT CACCTCCACT 
TCTCACAGCA ACTTCAGATA CTTCGTCCTG GATAACTCCG TCATCCTCGC CATGCTGGAG 
CAGCCGCTGG GGAACGAACA GAATCCCTGT CCGTCTGTCA CCATGTTAAT TAGAGGTATG 
TCTGGCCGAC ATGCATGGAC TATGCAGCTT TTCCATCAGC CTCGAGGAGC AAGAGCCAAT 
CAGAAGCAAG TGTTTGTTCC TGAAAGTCGA CCCACTCCCA AAAATGATGT TGGGATTCGG 
TTTAACGTCA AGCACAGGCC GTTCCCAGAA GAAGTAGATA AGATTCCCTT TGTGAAAGCC 
GACCTGAGCA TCCCAGACCT CCATGACATT GTGGACAAAG AGCTGGAGCA GCAGCATGAT 
AAGCTGCGAG TGGTGATGGG GAAGCAGATC GATTACGAGA CCAAACTAGA GCACCATACT 
GAGGACCTGT GGAGGAATAA AGCCTTCCCA GACCCACTGA CCGACTGTAA ACCGCCAGCT 
CCTGCTCAGG AGTTCCAGAC TGCTCGCCTT TTCCTGTCAC ACTTTGGCTT CCTGTCACTA 
GAGGCGCTCA AGGAGCCTGG AAACAGTCGT CTTCCTCCTC ACCTCATCGC TCTGGACTCT 
TCAGTGCCGG GTTTTTTCGA TGATGTGGGA TATCTGGACC TGCTGCCGTC CCGGCCGTGT 
GACACCGTCT TCATCTTCTA CATGAGAGCA GGACAGAAGA GCAGCCAGGA GATCTTGAAG 
AACGTGGAGT CCTCCGCAAA CGTGCAGCCA CATTTCCTGG AGCTGCTGCT GTCACTGGGC 
TGGCCCGTGG AGGTCGCTCA GCATCCAGGC TGGACGGGTA GTGTGTTCAC CAGCTGGACC 
ATCAACACAT CCAACGGCGC TGACACACCA GATGACTCTG TGACTCTGGG TGACACTGGC 
GGTGGTGTGT TTAACGGAGA GAAGCAGGTT CTCTATTACG CAGACGCTCT GACAGAAATC 
GCATTTGTGG TGCCGTCTCT CAGCGACTCC TCTGCGGAGT CATCAGAGCA CAGCTTCCCC 
ACCGCAGACT CCGACTCTCA GATGGAGCTG CTGCCCAGTT TACTCAAACA ACCCAACCTC 
ACGCTGGAGC TCTTCCCAAA CCACTCTGAC AACATGGGCC CTGCGCAACG GTCGCCCACA 
GCGAAAAACA AGAAGTTGCC GCCCGGAAGG ACGATACCGC CGCTGGGACC CGACACCAAA 
GTGTTGGTGG TGTGGGTTGA GCGATACGAC GACATTGAAA ACTTCCCTGT GTCAGAGTTA 
CTAGCAGAGA CGAGCACCGG TGTGGAGACG GCCGTCAATA GCAGCGCATC CAGGTCCAGC 
TCCTCAGAGA AGGACGTCCC CATAGTGTTC ATCCATCCAC TGAAAACCGG CCTGTTCCGC 
ATCAAACTGC AGGGCGCTCT GGGAAAATTC AACATGGTCA TTCCTCTGGT GGACAACATG 
GTGGTCAGCA GGAGATCACT GGGTTTTCTG GTGCGTCAGA CTGTAATCAA CGCGTGTCGG 
CGGAAGCGTC TGGAGAGCGA CTCCTACAGT CCTCCACACG TGCGGCGGAA ACAGAAGATC 
GCAGAAATCG TGAACCGTTA CCGAAACAAG CAGCTGGAGC CCGAGTTTTA CACCTCGCTC 
TTCCTGGAGG TCAACGAGAC CAGCCTCAAA CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333003.1
CDS	210..4865
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGVTVSRSNIRDRLPSIGVAVSRNNLRERLPSYGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTATFFQILLSYKSSLATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_021477328.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477328.1

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCAACATTT 
TTCCAAATTC TTCTTTCTTA TAAATCTTCA CTGGCCACTC AAGCTGGAGT TGAACTGAGG 
CGTAAGGGCT CTCAGATGTC CACAGACACC ATGGTCTCCA ACCCCATGTT TGACGCCAAT 
GAATTTCCTG ATAATTATGA GTCGGGTCGA GCTGAAGCAT GCGGGACGCT CTGCAGGATC 
TTCTGCAGTA AGAAAACAGG AGAGGAGATT CTTCCCGTCT ACCTGTCCAG GTTCTACATG 
GTGCTGATCC AGGGTCTTCA GATTTCAGAC TTCATCTGCA GGCCGGTCTT AGCCAGCATT 
ATCCTGAACT CTTCTGCTCT GTTCTGCTCC GATCTCAAGG GAATCAATGT AGTGGTTCCC 
TACTTCATAT CTGCACTGGA GACCATCCTG CCGGACCGAG AGCTGTCGAA GTTTAAGTCT 
TATGTGAATC CTACTGAGCT GAGAAGAGCC TCCATCCACA TCCTGCTGTC CATGCTTCCG 
CTTCCTCACC ACTTCGGCAA CGTCAAATCA GAGGTTCTGT TGGAAGGGAA ATTCAGCAAT 
GATGACAACT CTCTCCACGA CAAGGCGGTC ACCTTCCTCT CCCTGAAGCT GCGATTGGTC 
AATATTCTGA TCGGCGCCCT GCAAACAGAG ACAGACCCGA TCAATACACA GATGATACTG 
GCGGCGATGC TGAATATCGT GCAGGATTCT GCTCTGCTGG AGTCTGTTGG TGCTCAGGCT 
GAAGTGGGCA GTGTGGATGG CAGCCATTCT CTGGCTAAGA GTCACAGTCG TAATAACAGT 
GGCATCAGCA ATGCTAGCGC AGGCAGTTCA GAAGCCACCA CTCCAGACAG CGAACGCCCG 
GCACAGGCCC TGCTGAGAGA CTACGCTCTC CACACAGACA CTGCGGCCGG GCTGTTGATC 
CGCAGCATTC ACCTGCTCAC CCAGAGACTC AACTCTCAGT GGAGACTCGA CATGAGCATC 
TCTCTGGCGG CGCTCGAGCT GCTCGCGGGA CTCGCCAAGG TGAAGGTGAG CGTGGAGTCG 
TCTGACCGCA AGCGTGCCGT CAGCTCGGTC TGCAGTTATA TTGTGTATCA ATGCAGTCGT 
CCAGCTCCGC TTCATTCCAG AGATCTGCAC TCCATGATCG TCGCTGCTTT CCACTGTCTG 
TGTGTGTGGC TTACTGAACA CCCCGACATG CTCAATGAGA AGGATTGTCT GATTGAAGTG 
TTGGAGATCG TGGAGCTCGG CATCTCCGGC AGCAAGTCCA AAACAGGCGA ACAGGAAGTG 
AAGTATAAAG GAGACAAAGA GCACAACCCT GCATCTATGA GGGTCAAAGA TGCCGCAGAG 
GCCACGCTAT CATGTATAAT GCAGGTTTTG GGCGCGTTCC CATCTCCCAG CGGTCCCGCA 
TCGCCCTGCA GTCTGCTGAA CGAGGACACG CTCATCAAAT ACTCCAGACT CACCTCCACT 
TCTCACAGCA ACTTCAGATA CTTCGTCCTG GATAACTCCG TCATCCTCGC CATGCTGGAG 
CAGCCGCTGG GGAACGAACA GAATCCCTGT CCGTCTGTCA CCATGTTAAT TAGAGGTATG 
TCTGGCCGAC ATGCATGGAC TATGCAGCTT TTCCATCAGC CTCGAGGAGC AAGAGCCAAT 
CAGAAGCAAG TGTTTGTTCC TGAAAGTCGA CCCACTCCCA AAAATGATGT TGGGATTCGG 
TTTAACGTCA AGCACAGGCC GTTCCCAGAA GAAGTAGATA AGATTCCCTT TGTGAAAGCC 
GACCTGAGCA TCCCAGACCT CCATGACATT GTGGACAAAG AGCTGGAGCA GCAGCATGAT 
AAGCTGCGAG TGGTGATGGG GAAGCAGATC GATTACGAGA CCAAACTAGA GCACCATACT 
GAGGACCTGT GGAGGAATAA AGCCTTCCCA GACCCACTGA CCGACTGTAA ACCGCCAGCT 
CCTGCTCAGG AGTTCCAGAC TGCTCGCCTT TTCCTGTCAC ACTTTGGCTT CCTGTCACTA 
GAGGCGCTCA AGGAGCCTGG AAACAGTCGT CTTCCTCCTC ACCTCATCGC TCTGGACTCT 
TCAGTGCCGG GTTTTTTCGA TGATGTGGGA TATCTGGACC TGCTGCCGTC CCGGCCGTGT 
GACACCGTCT TCATCTTCTA CATGAGAGCA GGACAGAAGA GCAGCCAGGA GATCTTGAAG 
AACGTGGAGT CCTCCGCAAA CGTGCAGCCA CATTTCCTGG AGCTGCTGCT GTCACTGGGC 
TGGCCCGTGG AGGTCGCTCA GCATCCAGGC TGGACGGGTA GTGTGTTCAC CAGCTGGACC 
ATCAACACAT CCAACGGCGC TGACACACCA GATGACTCTG TGACTCTGGG TGACACTGGC 
GGTGGTGTGT TTAACGGAGA GAAGCAGGTT CTCTATTACG CAGACGCTCT GACAGAAATC 
GCATTTGTGG TGCCGTCTCT CAGCGACTCC TCTGCGGAGT CATCAGAGCA CAGCTTCCCC 
ACCGCAGACT CCGACTCTCA GATGGAGCTG CTGCCCAGTT TACTCAAACA ACCCAACCTC 
ACGCTGGAGC TCTTCCCAAA CCACTCTGAC AACATGGGCC CTGCGCAACG GTCGCCCACA 
GCGAAAAACA AGAAGTTGCC GCCCGGAAGG ACGATACCGC CGCTGGGACC CGACACCAAA 
GTGTTGGTGG TGTGGGTTGA GCGATACGAC GACATTGAAA ACTTCCCTGT GTCAGAGTTA 
CTAGCAGAGA CGAGCACCGG TGTGGAGACG GCCGTCAATA GCAGCGCATC CAGGTCCAGC 
TCCTCAGAGA AGGACGTCCC CATAGTGTTC ATCCATCCAC TGAAAACCGG CCTGTTCCGC 
ATCAAACTGC AGGGCGCTCT GGGAAAATTC AACATGGTCA TTCCTCTGGT GGACAACATG 
GTGGTCAGCA GGAGATCACT GGGTTTTCTG GTGCGTCAGA CTGTAATCAA CGCGTGTCGG 
CGGAAGCGTC TGGAGAGCGA CTCCTACAGT CCTCCACACG TGCGGCGGAA ACAGAAGATC 
GCAGAAATCG TGAACCGTTA CCGAAACAAG CAGCTGGAGC CCGAGTTTTA CACCTCGCTC 
TTCCTGGAGG TCAACGAGAC CAGCCTCAAA CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_021477330.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333005.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

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CloneID	ODa143151
Clone ID Related Accession (Same CDS sequence)	XM_021477330.1
Accession Version	XM_021477330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGTAT ATCCCGCCCC CGCTCTGACA GTGCTCCGCC CACTCCTGTC 
AATAGGCTCA GTATGCCACC GCCCCCAACA ACAACCAACA CAACACCGCC TCACAACCGC 
AGGCACCGCC CCACTGCTGT TGCCAAGACA ACCAGCAAAT CCTCAACGAC TGGGCAGACC 
AGAGTATCGC ATCAGCCTTC GTCCACATCT CCTCTCTCCA GCCCAAACCA GACGAGCTCC 
GAGCCCCGTC CGCTCCCGGC TCCGACCCGG CCCAAAGTCA ACAGCGTGTT AAATCTGTTC 
GGCCAGTGGC TGTTTGACGC TGCGCTGGTC CACTGTAAAC TGCACTCCGG CCTGAGCAGA 
GACAGCAGCA TGACTGCAAC ATTTTTCCAA ATTCTTCTTT CTTATAAATC TTCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333005.1
CDS	210..4769
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTATFFQILLSYKSSLATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_021477330.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477330.1

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGTAT ATCCCGCCCC CGCTCTGACA GTGCTCCGCC CACTCCTGTC 
AATAGGCTCA GTATGCCACC GCCCCCAACA ACAACCAACA CAACACCGCC TCACAACCGC 
AGGCACCGCC CCACTGCTGT TGCCAAGACA ACCAGCAAAT CCTCAACGAC TGGGCAGACC 
AGAGTATCGC ATCAGCCTTC GTCCACATCT CCTCTCTCCA GCCCAAACCA GACGAGCTCC 
GAGCCCCGTC CGCTCCCGGC TCCGACCCGG CCCAAAGTCA ACAGCGTGTT AAATCTGTTC 
GGCCAGTGGC TGTTTGACGC TGCGCTGGTC CACTGTAAAC TGCACTCCGG CCTGAGCAGA 
GACAGCAGCA TGACTGCAAC ATTTTTCCAA ATTCTTCTTT CTTATAAATC TTCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_021477331.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333006.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

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CloneID	ODa143152
Clone ID Related Accession (Same CDS sequence)	XM_021477331.1
Accession Version	XM_021477331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCAACA TTTTTCCAAA TTCTTCTTTC TTATAAATCT 
TCACTGGCCA CTCAAGCTGG AGTTGAACTG AGGCGTAAGG GCTCTCAGAT GTCCACAGAC 
ACCATGGTCT CCAACCCCAT GTTTGACGCC AATGAATTTC CTGATAATTA TGAGTCGGGT 
CGAGCTGAAG CATGCGGGAC GCTCTGCAGG ATCTTCTGCA GTAAGAAAAC AGGAGAGGAG 
ATTCTTCCCG TCTACCTGTC CAGGTTCTAC ATGGTGCTGA TCCAGGGTCT TCAGATTTCA 
GACTTCATCT GCAGGCCGGT CTTAGCCAGC ATTATCCTGA ACTCTTCTGC TCTGTTCTGC 
TCCGATCTCA AGGGAATCAA TGTAGTGGTT CCCTACTTCA TATCTGCACT GGAGACCATC 
CTGCCGGACC GAGAGCTGTC GAAGTTTAAG TCTTATGTGA ATCCTACTGA GCTGAGAAGA 
GCCTCCATCC ACATCCTGCT GTCCATGCTT CCGCTTCCTC ACCACTTCGG CAACGTCAAA 
TCAGAGGTTC TGTTGGAAGG GAAATTCAGC AATGATGACA ACTCTCTCCA CGACAAGGCG 
GTCACCTTCC TCTCCCTGAA GCTGCGATTG GTCAATATTC TGATCGGCGC CCTGCAAACA 
GAGACAGACC CGATCAATAC ACAGATGATA CTGGCGGCGA TGCTGAATAT CGTGCAGGAT 
TCTGCTCTGC TGGAGTCTGT TGGTGCTCAG GCTGAAGTGG GCAGTGTGGA TGGCAGCCAT 
TCTCTGGCTA AGAGTCACAG TCGTAATAAC AGTGGCATCA GCAATGCTAG CGCAGGCAGT 
TCAGAAGCCA CCACTCCAGA CAGCGAACGC CCGGCACAGG CCCTGCTGAG AGACTACGCT 
CTCCACACAG ACACTGCGGC CGGGCTGTTG ATCCGCAGCA TTCACCTGCT CACCCAGAGA 
CTCAACTCTC AGTGGAGACT CGACATGAGC ATCTCTCTGG CGGCGCTCGA GCTGCTCGCG 
GGACTCGCCA AGGTGAAGGT GAGCGTGGAG TCGTCTGACC GCAAGCGTGC CGTCAGCTCG 
GTCTGCAGTT ATATTGTGTA TCAATGCAGT CGTCCAGCTC CGCTTCATTC CAGAGATCTG 
CACTCCATGA TCGTCGCTGC TTTCCACTGT CTGTGTGTGT GGCTTACTGA ACACCCCGAC 
ATGCTCAATG AGAAGGATTG TCTGATTGAA GTGTTGGAGA TCGTGGAGCT CGGCATCTCC 
GGCAGCAAGT CCAAAACAGG CGAACAGGAA GTGAAGTATA AAGGAGACAA AGAGCACAAC 
CCTGCATCTA TGAGGGTCAA AGATGCCGCA GAGGCCACGC TATCATGTAT AATGCAGGTT 
TTGGGCGCGT TCCCATCTCC CAGCGGTCCC GCATCGCCCT GCAGTCTGCT GAACGAGGAC 
ACGCTCATCA AATACTCCAG ACTCACCTCC ACTTCTCACA GCAACTTCAG ATACTTCGTC 
CTGGATAACT CCGTCATCCT CGCCATGCTG GAGCAGCCGC TGGGGAACGA ACAGAATCCC 
TGTCCGTCTG TCACCATGTT AATTAGAGGT ATGTCTGGCC GACATGCATG GACTATGCAG 
CTTTTCCATC AGCCTCGAGG AGCAAGAGCC AATCAGAAGC AAGTGTTTGT TCCTGAAAGT 
CGACCCACTC CCAAAAATGA TGTTGGGATT CGGTTTAACG TCAAGCACAG GCCGTTCCCA 
GAAGAAGTAG ATAAGATTCC CTTTGTGAAA GCCGACCTGA GCATCCCAGA CCTCCATGAC 
ATTGTGGACA AAGAGCTGGA GCAGCAGCAT GATAAGCTGC GAGTGGTGAT GGGGAAGCAG 
ATCGATTACG AGACCAAACT AGAGCACCAT ACTGAGGACC TGTGGAGGAA TAAAGCCTTC 
CCAGACCCAC TGACCGACTG TAAACCGCCA GCTCCTGCTC AGGAGTTCCA GACTGCTCGC 
CTTTTCCTGT CACACTTTGG CTTCCTGTCA CTAGAGGCGC TCAAGGAGCC TGGAAACAGT 
CGTCTTCCTC CTCACCTCAT CGCTCTGGAC TCTTCAGTGC CGGGTTTTTT CGATGATGTG 
GGATATCTGG ACCTGCTGCC GTCCCGGCCG TGTGACACCG TCTTCATCTT CTACATGAGA 
GCAGGACAGA AGAGCAGCCA GGAGATCTTG AAGAACGTGG AGTCCTCCGC AAACGTGCAG 
CCACATTTCC TGGAGCTGCT GCTGTCACTG GGCTGGCCCG TGGAGGTCGC TCAGCATCCA 
GGCTGGACGG GTAGTGTGTT CACCAGCTGG ACCATCAACA CATCCAACGG CGCTGACACA 
CCAGATGACT CTGTGACTCT GGGTGACACT GGCGGTGGTG TGTTTAACGG AGAGAAGCAG 
GTTCTCTATT ACGCAGACGC TCTGACAGAA ATCGCATTTG TGGTGCCGTC TCTCAGCGAC 
TCCTCTGCGG AGTCATCAGA GCACAGCTTC CCCACCGCAG ACTCCGACTC TCAGATGGAG 
CTGCTGCCCA GTTTACTCAA ACAACCCAAC CTCACGCTGG AGCTCTTCCC AAACCACTCT 
GACAACATGG GCCCTGCGCA ACGGTCGCCC ACAGCGAAAA ACAAGAAGTT GCCGCCCGGA 
AGGACGATAC CGCCGCTGGG ACCCGACACC AAAGTGTTGG TGGTGTGGGT TGAGCGATAC 
GACGACATTG AAAACTTCCC TGTGTCAGAG TTACTAGCAG AGACGAGCAC CGGTGTGGAG 
ACGGCCGTCA ATAGCAGCGC ATCCAGGTCC AGCTCCTCAG AGAAGGACGT CCCCATAGTG 
TTCATCCATC CACTGAAAAC CGGCCTGTTC CGCATCAAAC TGCAGGGCGC TCTGGGAAAA 
TTCAACATGG TCATTCCTCT GGTGGACAAC ATGGTGGTCA GCAGGAGATC ACTGGGTTTT 
CTGGTGCGTC AGACTGTAAT CAACGCGTGT CGGCGGAAGC GTCTGGAGAG CGACTCCTAC 
AGTCCTCCAC ACGTGCGGCG GAAACAGAAG ATCGCAGAAA TCGTGAACCG TTACCGAAAC 
AAGCAGCTGG AGCCCGAGTT TTACACCTCG CTCTTCCTGG AGGTCAACGA GACCAGCCTC 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333006.1
CDS	209..4717
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTATFFQILLSYKSSLATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_021477331.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477331.1

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCAACA TTTTTCCAAA TTCTTCTTTC TTATAAATCT 
TCACTGGCCA CTCAAGCTGG AGTTGAACTG AGGCGTAAGG GCTCTCAGAT GTCCACAGAC 
ACCATGGTCT CCAACCCCAT GTTTGACGCC AATGAATTTC CTGATAATTA TGAGTCGGGT 
CGAGCTGAAG CATGCGGGAC GCTCTGCAGG ATCTTCTGCA GTAAGAAAAC AGGAGAGGAG 
ATTCTTCCCG TCTACCTGTC CAGGTTCTAC ATGGTGCTGA TCCAGGGTCT TCAGATTTCA 
GACTTCATCT GCAGGCCGGT CTTAGCCAGC ATTATCCTGA ACTCTTCTGC TCTGTTCTGC 
TCCGATCTCA AGGGAATCAA TGTAGTGGTT CCCTACTTCA TATCTGCACT GGAGACCATC 
CTGCCGGACC GAGAGCTGTC GAAGTTTAAG TCTTATGTGA ATCCTACTGA GCTGAGAAGA 
GCCTCCATCC ACATCCTGCT GTCCATGCTT CCGCTTCCTC ACCACTTCGG CAACGTCAAA 
TCAGAGGTTC TGTTGGAAGG GAAATTCAGC AATGATGACA ACTCTCTCCA CGACAAGGCG 
GTCACCTTCC TCTCCCTGAA GCTGCGATTG GTCAATATTC TGATCGGCGC CCTGCAAACA 
GAGACAGACC CGATCAATAC ACAGATGATA CTGGCGGCGA TGCTGAATAT CGTGCAGGAT 
TCTGCTCTGC TGGAGTCTGT TGGTGCTCAG GCTGAAGTGG GCAGTGTGGA TGGCAGCCAT 
TCTCTGGCTA AGAGTCACAG TCGTAATAAC AGTGGCATCA GCAATGCTAG CGCAGGCAGT 
TCAGAAGCCA CCACTCCAGA CAGCGAACGC CCGGCACAGG CCCTGCTGAG AGACTACGCT 
CTCCACACAG ACACTGCGGC CGGGCTGTTG ATCCGCAGCA TTCACCTGCT CACCCAGAGA 
CTCAACTCTC AGTGGAGACT CGACATGAGC ATCTCTCTGG CGGCGCTCGA GCTGCTCGCG 
GGACTCGCCA AGGTGAAGGT GAGCGTGGAG TCGTCTGACC GCAAGCGTGC CGTCAGCTCG 
GTCTGCAGTT ATATTGTGTA TCAATGCAGT CGTCCAGCTC CGCTTCATTC CAGAGATCTG 
CACTCCATGA TCGTCGCTGC TTTCCACTGT CTGTGTGTGT GGCTTACTGA ACACCCCGAC 
ATGCTCAATG AGAAGGATTG TCTGATTGAA GTGTTGGAGA TCGTGGAGCT CGGCATCTCC 
GGCAGCAAGT CCAAAACAGG CGAACAGGAA GTGAAGTATA AAGGAGACAA AGAGCACAAC 
CCTGCATCTA TGAGGGTCAA AGATGCCGCA GAGGCCACGC TATCATGTAT AATGCAGGTT 
TTGGGCGCGT TCCCATCTCC CAGCGGTCCC GCATCGCCCT GCAGTCTGCT GAACGAGGAC 
ACGCTCATCA AATACTCCAG ACTCACCTCC ACTTCTCACA GCAACTTCAG ATACTTCGTC 
CTGGATAACT CCGTCATCCT CGCCATGCTG GAGCAGCCGC TGGGGAACGA ACAGAATCCC 
TGTCCGTCTG TCACCATGTT AATTAGAGGT ATGTCTGGCC GACATGCATG GACTATGCAG 
CTTTTCCATC AGCCTCGAGG AGCAAGAGCC AATCAGAAGC AAGTGTTTGT TCCTGAAAGT 
CGACCCACTC CCAAAAATGA TGTTGGGATT CGGTTTAACG TCAAGCACAG GCCGTTCCCA 
GAAGAAGTAG ATAAGATTCC CTTTGTGAAA GCCGACCTGA GCATCCCAGA CCTCCATGAC 
ATTGTGGACA AAGAGCTGGA GCAGCAGCAT GATAAGCTGC GAGTGGTGAT GGGGAAGCAG 
ATCGATTACG AGACCAAACT AGAGCACCAT ACTGAGGACC TGTGGAGGAA TAAAGCCTTC 
CCAGACCCAC TGACCGACTG TAAACCGCCA GCTCCTGCTC AGGAGTTCCA GACTGCTCGC 
CTTTTCCTGT CACACTTTGG CTTCCTGTCA CTAGAGGCGC TCAAGGAGCC TGGAAACAGT 
CGTCTTCCTC CTCACCTCAT CGCTCTGGAC TCTTCAGTGC CGGGTTTTTT CGATGATGTG 
GGATATCTGG ACCTGCTGCC GTCCCGGCCG TGTGACACCG TCTTCATCTT CTACATGAGA 
GCAGGACAGA AGAGCAGCCA GGAGATCTTG AAGAACGTGG AGTCCTCCGC AAACGTGCAG 
CCACATTTCC TGGAGCTGCT GCTGTCACTG GGCTGGCCCG TGGAGGTCGC TCAGCATCCA 
GGCTGGACGG GTAGTGTGTT CACCAGCTGG ACCATCAACA CATCCAACGG CGCTGACACA 
CCAGATGACT CTGTGACTCT GGGTGACACT GGCGGTGGTG TGTTTAACGG AGAGAAGCAG 
GTTCTCTATT ACGCAGACGC TCTGACAGAA ATCGCATTTG TGGTGCCGTC TCTCAGCGAC 
TCCTCTGCGG AGTCATCAGA GCACAGCTTC CCCACCGCAG ACTCCGACTC TCAGATGGAG 
CTGCTGCCCA GTTTACTCAA ACAACCCAAC CTCACGCTGG AGCTCTTCCC AAACCACTCT 
GACAACATGG GCCCTGCGCA ACGGTCGCCC ACAGCGAAAA ACAAGAAGTT GCCGCCCGGA 
AGGACGATAC CGCCGCTGGG ACCCGACACC AAAGTGTTGG TGGTGTGGGT TGAGCGATAC 
GACGACATTG AAAACTTCCC TGTGTCAGAG TTACTAGCAG AGACGAGCAC CGGTGTGGAG 
ACGGCCGTCA ATAGCAGCGC ATCCAGGTCC AGCTCCTCAG AGAAGGACGT CCCCATAGTG 
TTCATCCATC CACTGAAAAC CGGCCTGTTC CGCATCAAAC TGCAGGGCGC TCTGGGAAAA 
TTCAACATGG TCATTCCTCT GGTGGACAAC ATGGTGGTCA GCAGGAGATC ACTGGGTTTT 
CTGGTGCGTC AGACTGTAAT CAACGCGTGT CGGCGGAAGC GTCTGGAGAG CGACTCCTAC 
AGTCCTCCAC ACGTGCGGCG GAAACAGAAG ATCGCAGAAA TCGTGAACCG TTACCGAAAC 
AAGCAGCTGG AGCCCGAGTT TTACACCTCG CTCTTCCTGG AGGTCAACGA GACCAGCCTC 
AAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302762.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301037.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43042
Clone ID Related Accession (Same CDS sequence)	XM_009302762.3 , XM_009302762.2
Accession Version	XM_009302762.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302762.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301037.1
CDS	209..4828
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGVTVSRSNIRDRLPSIGVAVSRNNLRERLPSYGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302762.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302762.3

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302762.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301037.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43042
Clone ID Related Accession (Same CDS sequence)	XM_009302762.3 , XM_009302762.2
Accession Version	XM_009302762.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301037.1
CDS	185..4804
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGVTVSRSNIRDRLPSIGVAVSRNNLRERLPSYGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302762.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302762.2

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGC TCTCCACACA 
GACACTGCGG CCGGGCTGTT GATCCGCAGC ATTCACCTGC TCACCCAGAG ACTCAACTCT 
CAGTGGAGAC TCGACATGAG CATCTCTCTG GCGGCGCTCG AGCTGCTCGC GGGACTCGCC 
AAGGTGAAGG TGAGCGTGGA GTCGTCTGAC CGCAAGCGTG CCGTCAGCTC GGTCTGCAGT 
TATATTGTGT ATCAATGCAG TCGTCCAGCT CCGCTTCATT CCAGAGATCT GCACTCCATG 
ATCGTCGCTG CTTTCCACTG TCTGTGTGTG TGGCTTACTG AACACCCCGA CATGCTCAAT 
GAGAAGGATT GTCTGATTGA AGTGTTGGAG ATCGTGGAGC TCGGCATCTC CGGCAGCAAG 
TCCAAAACAG GCGAACAGGA AGTGAAGTAT AAAGGAGACA AAGAGCACAA CCCTGCATCT 
ATGAGGGTCA AAGATGCCGC AGAGGCCACG CTATCATGTA TAATGCAGGT TTTGGGCGCG 
TTCCCATCTC CCAGCGGTCC CGCATCGCCC TGCAGTCTGC TGAACGAGGA CACGCTCATC 
AAATACTCCA GACTCACCTC CACTTCTCAC AGCAACTTCA GATACTTCGT CCTGGATAAC 
TCCGTCATCC TCGCCATGCT GGAGCAGCCG CTGGGGAACG AACAGAATCC CTGTCCGTCT 
GTCACCATGT TAATTAGAGG TATGTCTGGC CGACATGCAT GGACTATGCA GCTTTTCCAT 
CAGCCTCGAG GAGCAAGAGC CAATCAGAAG CAAGTGTTTG TTCCTGAAAG TCGACCCACT 
CCCAAAAATG ATGTTGGGAT TCGGTTTAAC GTCAAGCACA GGCCGTTCCC AGAAGAAGTA 
GATAAGATTC CCTTTGTGAA AGCCGACCTG AGCATCCCAG ACCTCCATGA CATTGTGGAC 
AAAGAGCTGG AGCAGCAGCA TGATAAGCTG CGAGTGGTGA TGGGGAAGCA GATCGATTAC 
GAGACCAAAC TAGAGCACCA TACTGAGGAC CTGTGGAGGA ATAAAGCCTT CCCAGACCCA 
CTGACCGACT GTAAACCGCC AGCTCCTGCT CAGGAGTTCC AGACTGCTCG CCTTTTCCTG 
TCACACTTTG GCTTCCTGTC ACTAGAGGCG CTCAAGGAGC CTGGAAACAG TCGTCTTCCT 
CCTCACCTCA TCGCTCTGGA CTCTTCAGTG CCGGGTTTTT TCGATGATGT GGGATATCTG 
GACCTGCTGC CGTCCCGGCC GTGTGACACC GTCTTCATCT TCTACATGAG AGCAGGACAG 
AAGAGCAGCC AGGAGATCTT GAAGAACGTG GAGTCCTCCG CAAACGTGCA GCCACATTTC 
CTGGAGCTGC TGCTGTCACT GGGCTGGCCC GTGGAGGTCG CTCAGCATCC AGGCTGGACG 
GGTAGTGTGT TCACCAGCTG GACCATCAAC ACATCCAACG GCGCTGACAC ACCAGATGAC 
TCTGTGACTC TGGGTGACAC TGGCGGTGGT GTGTTTAACG GAGAGAAGCA GGTTCTCTAT 
TACGCAGACG CTCTGACAGA AATCGCATTT GTGGTGCCGT CTCTCAGCGA CTCCTCTGCG 
GAGTCATCAG AGCACAGCTT CCCCACCGCA GACTCCGACT CTCAGATGGA GCTGCTGCCC 
AGTTTACTCA AACAACCCAA CCTCACGCTG GAGCTCTTCC CAAACCACTC TGACAACATG 
GGCCCTGCGC AACGGTCGCC CACAGCGAAA AACAAGAAGT TGCCGCCCGG AAGGACGATA 
CCGCCGCTGG GACCCGACAC CAAAGTGTTG GTGGTGTGGG TTGAGCGATA CGACGACATT 
GAAAACTTCC CTGTGTCAGA GTTACTAGCA GAGACGAGCA CCGGTGTGGA GACGGCCGTC 
AATAGCAGCG CATCCAGGTC CAGCTCCTCA GAGAAGGACG TCCCCATAGT GTTCATCCAT 
CCACTGAAAA CCGGCCTGTT CCGCATCAAA CTGCAGGGCG CTCTGGGAAA ATTCAACATG 
GTCATTCCTC TGGTGGACAA CATGGTGGTC AGCAGGAGAT CACTGGGTTT TCTGGTGCGT 
CAGACTGTAA TCAACGCGTG TCGGCGGAAG CGTCTGGAGA GCGACTCCTA CAGTCCTCCA 
CACGTGCGGC GGAAACAGAA GATCGCAGAA ATCGTGAACC GTTACCGAAA CAAGCAGCTG 
GAGCCCGAGT TTTACACCTC GCTCTTCCTG GAGGTCAACG AGACCAGCCT CAAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302763.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301038.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

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CloneID	ODa43043
Clone ID Related Accession (Same CDS sequence)	XM_009302763.2 , XM_009302763.3
Accession Version	XM_009302763.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301038.1
CDS	185..4792
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGVTVSRSNIRDRLPSIGVAVSRNNLRERLPSYGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302763.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302763.2

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302763.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301038.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

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CloneID	ODa43043
Clone ID Related Accession (Same CDS sequence)	XM_009302763.2 , XM_009302763.3
Accession Version	XM_009302763.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302763.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301038.1
CDS	210..4817
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGVAVTQKLECRETLPSSGVTVSRSNIRDRLPSIGVAVSRNNLRERLPSYGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302763.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302763.3

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTGTC GCTGTTACTC AGAAACTTGA GTGTCGTGAA 
ACTCTGCCCT CTTCTGGAGT CACAGTGTCT AGGAGCAATA TTCGAGACAG ACTTCCCTCC 
ATAGGTGTTG CAGTATCTCG GAATAATCTC CGAGAAAGGC TCCCGTCTTA TGGTATATCC 
CGCCCCCGCT CTGACAGTGC TCCGCCCACT CCTGTCAATA GGCTCAGTAT GCCACCGCCC 
CCAACAACAA CCAACACAAC ACCGCCTCAC AACCGCAGGC ACCGCCCCAC TGCTGTTGCC 
AAGACAACCA GCAAATCCTC AACGACTGGG CAGACCAGAG TATCGCATCA GCCTTCGTCC 
ACATCTCCTC TCTCCAGCCC AAACCAGACG AGCTCCGAGC CCCGTCCGCT CCCGGCTCCG 
ACCCGGCCCA AAGTCAACAG CGTGTTAAAT CTGTTCGGCC AGTGGCTGTT TGACGCTGCG 
CTGGTCCACT GTAAACTGCA CTCCGGCCTG AGCAGAGACA GCAGCATGAC TGCACTGGCC 
ACTCAAGCTG GAGTTGAACT GAGGCGTAAG GGCTCTCAGA TGTCCACAGA CACCATGGTC 
TCCAACCCCA TGTTTGACGC CAATGAATTT CCTGATAATT ATGAGTCGGG TCGAGCTGAA 
GCATGCGGGA CGCTCTGCAG GATCTTCTGC AGTAAGAAAA CAGGAGAGGA GATTCTTCCC 
GTCTACCTGT CCAGGTTCTA CATGGTGCTG ATCCAGGGTC TTCAGATTTC AGACTTCATC 
TGCAGGCCGG TCTTAGCCAG CATTATCCTG AACTCTTCTG CTCTGTTCTG CTCCGATCTC 
AAGGGAATCA ATGTAGTGGT TCCCTACTTC ATATCTGCAC TGGAGACCAT CCTGCCGGAC 
CGAGAGCTGT CGAAGTTTAA GTCTTATGTG AATCCTACTG AGCTGAGAAG AGCCTCCATC 
CACATCCTGC TGTCCATGCT TCCGCTTCCT CACCACTTCG GCAACGTCAA ATCAGAGGTT 
CTGTTGGAAG GGAAATTCAG CAATGATGAC AACTCTCTCC ACGACAAGGC GGTCACCTTC 
CTCTCCCTGA AGCTGCGATT GGTCAATATT CTGATCGGCG CCCTGCAAAC AGAGACAGAC 
CCGATCAATA CACAGATGAT ACTGGCGGCG ATGCTGAATA TCGTGCAGGA TTCTGCTCTG 
CTGGAGTCTG TTGGTGCTCA GGCTGAAGTG GGCAGTGTGG ATGGCAGCCA TTCTCTGGCT 
AAGAGTCACA GTCGTAATAA CAGTGGCATC AGCAATGCTA GCGCAGGCAG TTCAGAAGCC 
ACCACTCCAG ACAGCGAACG CCCGGCACAG GCCCTGCTGA GAGACTACGA CACTGCGGCC 
GGGCTGTTGA TCCGCAGCAT TCACCTGCTC ACCCAGAGAC TCAACTCTCA GTGGAGACTC 
GACATGAGCA TCTCTCTGGC GGCGCTCGAG CTGCTCGCGG GACTCGCCAA GGTGAAGGTG 
AGCGTGGAGT CGTCTGACCG CAAGCGTGCC GTCAGCTCGG TCTGCAGTTA TATTGTGTAT 
CAATGCAGTC GTCCAGCTCC GCTTCATTCC AGAGATCTGC ACTCCATGAT CGTCGCTGCT 
TTCCACTGTC TGTGTGTGTG GCTTACTGAA CACCCCGACA TGCTCAATGA GAAGGATTGT 
CTGATTGAAG TGTTGGAGAT CGTGGAGCTC GGCATCTCCG GCAGCAAGTC CAAAACAGGC 
GAACAGGAAG TGAAGTATAA AGGAGACAAA GAGCACAACC CTGCATCTAT GAGGGTCAAA 
GATGCCGCAG AGGCCACGCT ATCATGTATA ATGCAGGTTT TGGGCGCGTT CCCATCTCCC 
AGCGGTCCCG CATCGCCCTG CAGTCTGCTG AACGAGGACA CGCTCATCAA ATACTCCAGA 
CTCACCTCCA CTTCTCACAG CAACTTCAGA TACTTCGTCC TGGATAACTC CGTCATCCTC 
GCCATGCTGG AGCAGCCGCT GGGGAACGAA CAGAATCCCT GTCCGTCTGT CACCATGTTA 
ATTAGAGGTA TGTCTGGCCG ACATGCATGG ACTATGCAGC TTTTCCATCA GCCTCGAGGA 
GCAAGAGCCA ATCAGAAGCA AGTGTTTGTT CCTGAAAGTC GACCCACTCC CAAAAATGAT 
GTTGGGATTC GGTTTAACGT CAAGCACAGG CCGTTCCCAG AAGAAGTAGA TAAGATTCCC 
TTTGTGAAAG CCGACCTGAG CATCCCAGAC CTCCATGACA TTGTGGACAA AGAGCTGGAG 
CAGCAGCATG ATAAGCTGCG AGTGGTGATG GGGAAGCAGA TCGATTACGA GACCAAACTA 
GAGCACCATA CTGAGGACCT GTGGAGGAAT AAAGCCTTCC CAGACCCACT GACCGACTGT 
AAACCGCCAG CTCCTGCTCA GGAGTTCCAG ACTGCTCGCC TTTTCCTGTC ACACTTTGGC 
TTCCTGTCAC TAGAGGCGCT CAAGGAGCCT GGAAACAGTC GTCTTCCTCC TCACCTCATC 
GCTCTGGACT CTTCAGTGCC GGGTTTTTTC GATGATGTGG GATATCTGGA CCTGCTGCCG 
TCCCGGCCGT GTGACACCGT CTTCATCTTC TACATGAGAG CAGGACAGAA GAGCAGCCAG 
GAGATCTTGA AGAACGTGGA GTCCTCCGCA AACGTGCAGC CACATTTCCT GGAGCTGCTG 
CTGTCACTGG GCTGGCCCGT GGAGGTCGCT CAGCATCCAG GCTGGACGGG TAGTGTGTTC 
ACCAGCTGGA CCATCAACAC ATCCAACGGC GCTGACACAC CAGATGACTC TGTGACTCTG 
GGTGACACTG GCGGTGGTGT GTTTAACGGA GAGAAGCAGG TTCTCTATTA CGCAGACGCT 
CTGACAGAAA TCGCATTTGT GGTGCCGTCT CTCAGCGACT CCTCTGCGGA GTCATCAGAG 
CACAGCTTCC CCACCGCAGA CTCCGACTCT CAGATGGAGC TGCTGCCCAG TTTACTCAAA 
CAACCCAACC TCACGCTGGA GCTCTTCCCA AACCACTCTG ACAACATGGG CCCTGCGCAA 
CGGTCGCCCA CAGCGAAAAA CAAGAAGTTG CCGCCCGGAA GGACGATACC GCCGCTGGGA 
CCCGACACCA AAGTGTTGGT GGTGTGGGTT GAGCGATACG ACGACATTGA AAACTTCCCT 
GTGTCAGAGT TACTAGCAGA GACGAGCACC GGTGTGGAGA CGGCCGTCAA TAGCAGCGCA 
TCCAGGTCCA GCTCCTCAGA GAAGGACGTC CCCATAGTGT TCATCCATCC ACTGAAAACC 
GGCCTGTTCC GCATCAAACT GCAGGGCGCT CTGGGAAAAT TCAACATGGT CATTCCTCTG 
GTGGACAACA TGGTGGTCAG CAGGAGATCA CTGGGTTTTC TGGTGCGTCA GACTGTAATC 
AACGCGTGTC GGCGGAAGCG TCTGGAGAGC GACTCCTACA GTCCTCCACA CGTGCGGCGG 
AAACAGAAGA TCGCAGAAAT CGTGAACCGT TACCGAAACA AGCAGCTGGA GCCCGAGTTT 
TACACCTCGC TCTTCCTGGA GGTCAACGAG ACCAGCCTCA AACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302765.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301040.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

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CloneID	ODa43045
Clone ID Related Accession (Same CDS sequence)	XM_009302765.2 , XM_009302765.3
Accession Version	XM_009302765.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302765.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGCTCTCCAC ACAGACACTG CGGCCGGGCT GTTGATCCGC 
AGCATTCACC TGCTCACCCA GAGACTCAAC TCTCAGTGGA GACTCGACAT GAGCATCTCT 
CTGGCGGCGC TCGAGCTGCT CGCGGGACTC GCCAAGGTGA AGGTGAGCGT GGAGTCGTCT 
GACCGCAAGC GTGCCGTCAG CTCGGTCTGC AGTTATATTG TGTATCAATG CAGTCGTCCA 
GCTCCGCTTC ATTCCAGAGA TCTGCACTCC ATGATCGTCG CTGCTTTCCA CTGTCTGTGT 
GTGTGGCTTA CTGAACACCC CGACATGCTC AATGAGAAGG ATTGTCTGAT TGAAGTGTTG 
GAGATCGTGG AGCTCGGCAT CTCCGGCAGC AAGTCCAAAA CAGGCGAACA GGAAGTGAAG 
TATAAAGGAG ACAAAGAGCA CAACCCTGCA TCTATGAGGG TCAAAGATGC CGCAGAGGCC 
ACGCTATCAT GTATAATGCA GGTTTTGGGC GCGTTCCCAT CTCCCAGCGG TCCCGCATCG 
CCCTGCAGTC TGCTGAACGA GGACACGCTC ATCAAATACT CCAGACTCAC CTCCACTTCT 
CACAGCAACT TCAGATACTT CGTCCTGGAT AACTCCGTCA TCCTCGCCAT GCTGGAGCAG 
CCGCTGGGGA ACGAACAGAA TCCCTGTCCG TCTGTCACCA TGTTAATTAG AGGTATGTCT 
GGCCGACATG CATGGACTAT GCAGCTTTTC CATCAGCCTC GAGGAGCAAG AGCCAATCAG 
AAGCAAGTGT TTGTTCCTGA AAGTCGACCC ACTCCCAAAA ATGATGTTGG GATTCGGTTT 
AACGTCAAGC ACAGGCCGTT CCCAGAAGAA GTAGATAAGA TTCCCTTTGT GAAAGCCGAC 
CTGAGCATCC CAGACCTCCA TGACATTGTG GACAAAGAGC TGGAGCAGCA GCATGATAAG 
CTGCGAGTGG TGATGGGGAA GCAGATCGAT TACGAGACCA AACTAGAGCA CCATACTGAG 
GACCTGTGGA GGAATAAAGC CTTCCCAGAC CCACTGACCG ACTGTAAACC GCCAGCTCCT 
GCTCAGGAGT TCCAGACTGC TCGCCTTTTC CTGTCACACT TTGGCTTCCT GTCACTAGAG 
GCGCTCAAGG AGCCTGGAAA CAGTCGTCTT CCTCCTCACC TCATCGCTCT GGACTCTTCA 
GTGCCGGGTT TTTTCGATGA TGTGGGATAT CTGGACCTGC TGCCGTCCCG GCCGTGTGAC 
ACCGTCTTCA TCTTCTACAT GAGAGCAGGA CAGAAGAGCA GCCAGGAGAT CTTGAAGAAC 
GTGGAGTCCT CCGCAAACGT GCAGCCACAT TTCCTGGAGC TGCTGCTGTC ACTGGGCTGG 
CCCGTGGAGG TCGCTCAGCA TCCAGGCTGG ACGGGTAGTG TGTTCACCAG CTGGACCATC 
AACACATCCA ACGGCGCTGA CACACCAGAT GACTCTGTGA CTCTGGGTGA CACTGGCGGT 
GGTGTGTTTA ACGGAGAGAA GCAGGTTCTC TATTACGCAG ACGCTCTGAC AGAAATCGCA 
TTTGTGGTGC CGTCTCTCAG CGACTCCTCT GCGGAGTCAT CAGAGCACAG CTTCCCCACC 
GCAGACTCCG ACTCTCAGAT GGAGCTGCTG CCCAGTTTAC TCAAACAACC CAACCTCACG 
CTGGAGCTCT TCCCAAACCA CTCTGACAAC ATGGGCCCTG CGCAACGGTC GCCCACAGCG 
AAAAACAAGA AGTTGCCGCC CGGAAGGACG ATACCGCCGC TGGGACCCGA CACCAAAGTG 
TTGGTGGTGT GGGTTGAGCG ATACGACGAC ATTGAAAACT TCCCTGTGTC AGAGTTACTA 
GCAGAGACGA GCACCGGTGT GGAGACGGCC GTCAATAGCA GCGCATCCAG GTCCAGCTCC 
TCAGAGAAGG ACGTCCCCAT AGTGTTCATC CATCCACTGA AAACCGGCCT GTTCCGCATC 
AAACTGCAGG GCGCTCTGGG AAAATTCAAC ATGGTCATTC CTCTGGTGGA CAACATGGTG 
GTCAGCAGGA GATCACTGGG TTTTCTGGTG CGTCAGACTG TAATCAACGC GTGTCGGCGG 
AAGCGTCTGG AGAGCGACTC CTACAGTCCT CCACACGTGC GGCGGAAACA GAAGATCGCA 
GAAATCGTGA ACCGTTACCG AAACAAGCAG CTGGAGCCCG AGTTTTACAC CTCGCTCTTC 
CTGGAGGTCA ACGAGACCAG CCTCAAACCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301040.1
CDS	185..4657
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302765.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302765.2

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGCTCTCCAC ACAGACACTG CGGCCGGGCT GTTGATCCGC 
AGCATTCACC TGCTCACCCA GAGACTCAAC TCTCAGTGGA GACTCGACAT GAGCATCTCT 
CTGGCGGCGC TCGAGCTGCT CGCGGGACTC GCCAAGGTGA AGGTGAGCGT GGAGTCGTCT 
GACCGCAAGC GTGCCGTCAG CTCGGTCTGC AGTTATATTG TGTATCAATG CAGTCGTCCA 
GCTCCGCTTC ATTCCAGAGA TCTGCACTCC ATGATCGTCG CTGCTTTCCA CTGTCTGTGT 
GTGTGGCTTA CTGAACACCC CGACATGCTC AATGAGAAGG ATTGTCTGAT TGAAGTGTTG 
GAGATCGTGG AGCTCGGCAT CTCCGGCAGC AAGTCCAAAA CAGGCGAACA GGAAGTGAAG 
TATAAAGGAG ACAAAGAGCA CAACCCTGCA TCTATGAGGG TCAAAGATGC CGCAGAGGCC 
ACGCTATCAT GTATAATGCA GGTTTTGGGC GCGTTCCCAT CTCCCAGCGG TCCCGCATCG 
CCCTGCAGTC TGCTGAACGA GGACACGCTC ATCAAATACT CCAGACTCAC CTCCACTTCT 
CACAGCAACT TCAGATACTT CGTCCTGGAT AACTCCGTCA TCCTCGCCAT GCTGGAGCAG 
CCGCTGGGGA ACGAACAGAA TCCCTGTCCG TCTGTCACCA TGTTAATTAG AGGTATGTCT 
GGCCGACATG CATGGACTAT GCAGCTTTTC CATCAGCCTC GAGGAGCAAG AGCCAATCAG 
AAGCAAGTGT TTGTTCCTGA AAGTCGACCC ACTCCCAAAA ATGATGTTGG GATTCGGTTT 
AACGTCAAGC ACAGGCCGTT CCCAGAAGAA GTAGATAAGA TTCCCTTTGT GAAAGCCGAC 
CTGAGCATCC CAGACCTCCA TGACATTGTG GACAAAGAGC TGGAGCAGCA GCATGATAAG 
CTGCGAGTGG TGATGGGGAA GCAGATCGAT TACGAGACCA AACTAGAGCA CCATACTGAG 
GACCTGTGGA GGAATAAAGC CTTCCCAGAC CCACTGACCG ACTGTAAACC GCCAGCTCCT 
GCTCAGGAGT TCCAGACTGC TCGCCTTTTC CTGTCACACT TTGGCTTCCT GTCACTAGAG 
GCGCTCAAGG AGCCTGGAAA CAGTCGTCTT CCTCCTCACC TCATCGCTCT GGACTCTTCA 
GTGCCGGGTT TTTTCGATGA TGTGGGATAT CTGGACCTGC TGCCGTCCCG GCCGTGTGAC 
ACCGTCTTCA TCTTCTACAT GAGAGCAGGA CAGAAGAGCA GCCAGGAGAT CTTGAAGAAC 
GTGGAGTCCT CCGCAAACGT GCAGCCACAT TTCCTGGAGC TGCTGCTGTC ACTGGGCTGG 
CCCGTGGAGG TCGCTCAGCA TCCAGGCTGG ACGGGTAGTG TGTTCACCAG CTGGACCATC 
AACACATCCA ACGGCGCTGA CACACCAGAT GACTCTGTGA CTCTGGGTGA CACTGGCGGT 
GGTGTGTTTA ACGGAGAGAA GCAGGTTCTC TATTACGCAG ACGCTCTGAC AGAAATCGCA 
TTTGTGGTGC CGTCTCTCAG CGACTCCTCT GCGGAGTCAT CAGAGCACAG CTTCCCCACC 
GCAGACTCCG ACTCTCAGAT GGAGCTGCTG CCCAGTTTAC TCAAACAACC CAACCTCACG 
CTGGAGCTCT TCCCAAACCA CTCTGACAAC ATGGGCCCTG CGCAACGGTC GCCCACAGCG 
AAAAACAAGA AGTTGCCGCC CGGAAGGACG ATACCGCCGC TGGGACCCGA CACCAAAGTG 
TTGGTGGTGT GGGTTGAGCG ATACGACGAC ATTGAAAACT TCCCTGTGTC AGAGTTACTA 
GCAGAGACGA GCACCGGTGT GGAGACGGCC GTCAATAGCA GCGCATCCAG GTCCAGCTCC 
TCAGAGAAGG ACGTCCCCAT AGTGTTCATC CATCCACTGA AAACCGGCCT GTTCCGCATC 
AAACTGCAGG GCGCTCTGGG AAAATTCAAC ATGGTCATTC CTCTGGTGGA CAACATGGTG 
GTCAGCAGGA GATCACTGGG TTTTCTGGTG CGTCAGACTG TAATCAACGC GTGTCGGCGG 
AAGCGTCTGG AGAGCGACTC CTACAGTCCT CCACACGTGC GGCGGAAACA GAAGATCGCA 
GAAATCGTGA ACCGTTACCG AAACAAGCAG CTGGAGCCCG AGTTTTACAC CTCGCTCTTC 
CTGGAGGTCA ACGAGACCAG CCTCAAACCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302765.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301040.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa43045
Clone ID Related Accession (Same CDS sequence)	XM_009302765.2 , XM_009302765.3
Accession Version	XM_009302765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGCTCTCCAC ACAGACACTG CGGCCGGGCT GTTGATCCGC 
AGCATTCACC TGCTCACCCA GAGACTCAAC TCTCAGTGGA GACTCGACAT GAGCATCTCT 
CTGGCGGCGC TCGAGCTGCT CGCGGGACTC GCCAAGGTGA AGGTGAGCGT GGAGTCGTCT 
GACCGCAAGC GTGCCGTCAG CTCGGTCTGC AGTTATATTG TGTATCAATG CAGTCGTCCA 
GCTCCGCTTC ATTCCAGAGA TCTGCACTCC ATGATCGTCG CTGCTTTCCA CTGTCTGTGT 
GTGTGGCTTA CTGAACACCC CGACATGCTC AATGAGAAGG ATTGTCTGAT TGAAGTGTTG 
GAGATCGTGG AGCTCGGCAT CTCCGGCAGC AAGTCCAAAA CAGGCGAACA GGAAGTGAAG 
TATAAAGGAG ACAAAGAGCA CAACCCTGCA TCTATGAGGG TCAAAGATGC CGCAGAGGCC 
ACGCTATCAT GTATAATGCA GGTTTTGGGC GCGTTCCCAT CTCCCAGCGG TCCCGCATCG 
CCCTGCAGTC TGCTGAACGA GGACACGCTC ATCAAATACT CCAGACTCAC CTCCACTTCT 
CACAGCAACT TCAGATACTT CGTCCTGGAT AACTCCGTCA TCCTCGCCAT GCTGGAGCAG 
CCGCTGGGGA ACGAACAGAA TCCCTGTCCG TCTGTCACCA TGTTAATTAG AGGTATGTCT 
GGCCGACATG CATGGACTAT GCAGCTTTTC CATCAGCCTC GAGGAGCAAG AGCCAATCAG 
AAGCAAGTGT TTGTTCCTGA AAGTCGACCC ACTCCCAAAA ATGATGTTGG GATTCGGTTT 
AACGTCAAGC ACAGGCCGTT CCCAGAAGAA GTAGATAAGA TTCCCTTTGT GAAAGCCGAC 
CTGAGCATCC CAGACCTCCA TGACATTGTG GACAAAGAGC TGGAGCAGCA GCATGATAAG 
CTGCGAGTGG TGATGGGGAA GCAGATCGAT TACGAGACCA AACTAGAGCA CCATACTGAG 
GACCTGTGGA GGAATAAAGC CTTCCCAGAC CCACTGACCG ACTGTAAACC GCCAGCTCCT 
GCTCAGGAGT TCCAGACTGC TCGCCTTTTC CTGTCACACT TTGGCTTCCT GTCACTAGAG 
GCGCTCAAGG AGCCTGGAAA CAGTCGTCTT CCTCCTCACC TCATCGCTCT GGACTCTTCA 
GTGCCGGGTT TTTTCGATGA TGTGGGATAT CTGGACCTGC TGCCGTCCCG GCCGTGTGAC 
ACCGTCTTCA TCTTCTACAT GAGAGCAGGA CAGAAGAGCA GCCAGGAGAT CTTGAAGAAC 
GTGGAGTCCT CCGCAAACGT GCAGCCACAT TTCCTGGAGC TGCTGCTGTC ACTGGGCTGG 
CCCGTGGAGG TCGCTCAGCA TCCAGGCTGG ACGGGTAGTG TGTTCACCAG CTGGACCATC 
AACACATCCA ACGGCGCTGA CACACCAGAT GACTCTGTGA CTCTGGGTGA CACTGGCGGT 
GGTGTGTTTA ACGGAGAGAA GCAGGTTCTC TATTACGCAG ACGCTCTGAC AGAAATCGCA 
TTTGTGGTGC CGTCTCTCAG CGACTCCTCT GCGGAGTCAT CAGAGCACAG CTTCCCCACC 
GCAGACTCCG ACTCTCAGAT GGAGCTGCTG CCCAGTTTAC TCAAACAACC CAACCTCACG 
CTGGAGCTCT TCCCAAACCA CTCTGACAAC ATGGGCCCTG CGCAACGGTC GCCCACAGCG 
AAAAACAAGA AGTTGCCGCC CGGAAGGACG ATACCGCCGC TGGGACCCGA CACCAAAGTG 
TTGGTGGTGT GGGTTGAGCG ATACGACGAC ATTGAAAACT TCCCTGTGTC AGAGTTACTA 
GCAGAGACGA GCACCGGTGT GGAGACGGCC GTCAATAGCA GCGCATCCAG GTCCAGCTCC 
TCAGAGAAGG ACGTCCCCAT AGTGTTCATC CATCCACTGA AAACCGGCCT GTTCCGCATC 
AAACTGCAGG GCGCTCTGGG AAAATTCAAC ATGGTCATTC CTCTGGTGGA CAACATGGTG 
GTCAGCAGGA GATCACTGGG TTTTCTGGTG CGTCAGACTG TAATCAACGC GTGTCGGCGG 
AAGCGTCTGG AGAGCGACTC CTACAGTCCT CCACACGTGC GGCGGAAACA GAAGATCGCA 
GAAATCGTGA ACCGTTACCG AAACAAGCAG CTGGAGCCCG AGTTTTACAC CTCGCTCTTC 
CTGGAGGTCA ACGAGACCAG CCTCAAACCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301040.1
CDS	209..4681
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYALHTDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302765.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302765.3

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGCTCTCCAC ACAGACACTG CGGCCGGGCT GTTGATCCGC 
AGCATTCACC TGCTCACCCA GAGACTCAAC TCTCAGTGGA GACTCGACAT GAGCATCTCT 
CTGGCGGCGC TCGAGCTGCT CGCGGGACTC GCCAAGGTGA AGGTGAGCGT GGAGTCGTCT 
GACCGCAAGC GTGCCGTCAG CTCGGTCTGC AGTTATATTG TGTATCAATG CAGTCGTCCA 
GCTCCGCTTC ATTCCAGAGA TCTGCACTCC ATGATCGTCG CTGCTTTCCA CTGTCTGTGT 
GTGTGGCTTA CTGAACACCC CGACATGCTC AATGAGAAGG ATTGTCTGAT TGAAGTGTTG 
GAGATCGTGG AGCTCGGCAT CTCCGGCAGC AAGTCCAAAA CAGGCGAACA GGAAGTGAAG 
TATAAAGGAG ACAAAGAGCA CAACCCTGCA TCTATGAGGG TCAAAGATGC CGCAGAGGCC 
ACGCTATCAT GTATAATGCA GGTTTTGGGC GCGTTCCCAT CTCCCAGCGG TCCCGCATCG 
CCCTGCAGTC TGCTGAACGA GGACACGCTC ATCAAATACT CCAGACTCAC CTCCACTTCT 
CACAGCAACT TCAGATACTT CGTCCTGGAT AACTCCGTCA TCCTCGCCAT GCTGGAGCAG 
CCGCTGGGGA ACGAACAGAA TCCCTGTCCG TCTGTCACCA TGTTAATTAG AGGTATGTCT 
GGCCGACATG CATGGACTAT GCAGCTTTTC CATCAGCCTC GAGGAGCAAG AGCCAATCAG 
AAGCAAGTGT TTGTTCCTGA AAGTCGACCC ACTCCCAAAA ATGATGTTGG GATTCGGTTT 
AACGTCAAGC ACAGGCCGTT CCCAGAAGAA GTAGATAAGA TTCCCTTTGT GAAAGCCGAC 
CTGAGCATCC CAGACCTCCA TGACATTGTG GACAAAGAGC TGGAGCAGCA GCATGATAAG 
CTGCGAGTGG TGATGGGGAA GCAGATCGAT TACGAGACCA AACTAGAGCA CCATACTGAG 
GACCTGTGGA GGAATAAAGC CTTCCCAGAC CCACTGACCG ACTGTAAACC GCCAGCTCCT 
GCTCAGGAGT TCCAGACTGC TCGCCTTTTC CTGTCACACT TTGGCTTCCT GTCACTAGAG 
GCGCTCAAGG AGCCTGGAAA CAGTCGTCTT CCTCCTCACC TCATCGCTCT GGACTCTTCA 
GTGCCGGGTT TTTTCGATGA TGTGGGATAT CTGGACCTGC TGCCGTCCCG GCCGTGTGAC 
ACCGTCTTCA TCTTCTACAT GAGAGCAGGA CAGAAGAGCA GCCAGGAGAT CTTGAAGAAC 
GTGGAGTCCT CCGCAAACGT GCAGCCACAT TTCCTGGAGC TGCTGCTGTC ACTGGGCTGG 
CCCGTGGAGG TCGCTCAGCA TCCAGGCTGG ACGGGTAGTG TGTTCACCAG CTGGACCATC 
AACACATCCA ACGGCGCTGA CACACCAGAT GACTCTGTGA CTCTGGGTGA CACTGGCGGT 
GGTGTGTTTA ACGGAGAGAA GCAGGTTCTC TATTACGCAG ACGCTCTGAC AGAAATCGCA 
TTTGTGGTGC CGTCTCTCAG CGACTCCTCT GCGGAGTCAT CAGAGCACAG CTTCCCCACC 
GCAGACTCCG ACTCTCAGAT GGAGCTGCTG CCCAGTTTAC TCAAACAACC CAACCTCACG 
CTGGAGCTCT TCCCAAACCA CTCTGACAAC ATGGGCCCTG CGCAACGGTC GCCCACAGCG 
AAAAACAAGA AGTTGCCGCC CGGAAGGACG ATACCGCCGC TGGGACCCGA CACCAAAGTG 
TTGGTGGTGT GGGTTGAGCG ATACGACGAC ATTGAAAACT TCCCTGTGTC AGAGTTACTA 
GCAGAGACGA GCACCGGTGT GGAGACGGCC GTCAATAGCA GCGCATCCAG GTCCAGCTCC 
TCAGAGAAGG ACGTCCCCAT AGTGTTCATC CATCCACTGA AAACCGGCCT GTTCCGCATC 
AAACTGCAGG GCGCTCTGGG AAAATTCAAC ATGGTCATTC CTCTGGTGGA CAACATGGTG 
GTCAGCAGGA GATCACTGGG TTTTCTGGTG CGTCAGACTG TAATCAACGC GTGTCGGCGG 
AAGCGTCTGG AGAGCGACTC CTACAGTCCT CCACACGTGC GGCGGAAACA GAAGATCGCA 
GAAATCGTGA ACCGTTACCG AAACAAGCAG CTGGAGCCCG AGTTTTACAC CTCGCTCTTC 
CTGGAGGTCA ACGAGACCAG CCTCAAACCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302766.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301041.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa43046
Clone ID Related Accession (Same CDS sequence)	XM_009302766.3 , XM_009302766.2
Accession Version	XM_009302766.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGACACTGCG GCCGGGCTGT TGATCCGCAG CATTCACCTG 
CTCACCCAGA GACTCAACTC TCAGTGGAGA CTCGACATGA GCATCTCTCT GGCGGCGCTC 
GAGCTGCTCG CGGGACTCGC CAAGGTGAAG GTGAGCGTGG AGTCGTCTGA CCGCAAGCGT 
GCCGTCAGCT CGGTCTGCAG TTATATTGTG TATCAATGCA GTCGTCCAGC TCCGCTTCAT 
TCCAGAGATC TGCACTCCAT GATCGTCGCT GCTTTCCACT GTCTGTGTGT GTGGCTTACT 
GAACACCCCG ACATGCTCAA TGAGAAGGAT TGTCTGATTG AAGTGTTGGA GATCGTGGAG 
CTCGGCATCT CCGGCAGCAA GTCCAAAACA GGCGAACAGG AAGTGAAGTA TAAAGGAGAC 
AAAGAGCACA ACCCTGCATC TATGAGGGTC AAAGATGCCG CAGAGGCCAC GCTATCATGT 
ATAATGCAGG TTTTGGGCGC GTTCCCATCT CCCAGCGGTC CCGCATCGCC CTGCAGTCTG 
CTGAACGAGG ACACGCTCAT CAAATACTCC AGACTCACCT CCACTTCTCA CAGCAACTTC 
AGATACTTCG TCCTGGATAA CTCCGTCATC CTCGCCATGC TGGAGCAGCC GCTGGGGAAC 
GAACAGAATC CCTGTCCGTC TGTCACCATG TTAATTAGAG GTATGTCTGG CCGACATGCA 
TGGACTATGC AGCTTTTCCA TCAGCCTCGA GGAGCAAGAG CCAATCAGAA GCAAGTGTTT 
GTTCCTGAAA GTCGACCCAC TCCCAAAAAT GATGTTGGGA TTCGGTTTAA CGTCAAGCAC 
AGGCCGTTCC CAGAAGAAGT AGATAAGATT CCCTTTGTGA AAGCCGACCT GAGCATCCCA 
GACCTCCATG ACATTGTGGA CAAAGAGCTG GAGCAGCAGC ATGATAAGCT GCGAGTGGTG 
ATGGGGAAGC AGATCGATTA CGAGACCAAA CTAGAGCACC ATACTGAGGA CCTGTGGAGG 
AATAAAGCCT TCCCAGACCC ACTGACCGAC TGTAAACCGC CAGCTCCTGC TCAGGAGTTC 
CAGACTGCTC GCCTTTTCCT GTCACACTTT GGCTTCCTGT CACTAGAGGC GCTCAAGGAG 
CCTGGAAACA GTCGTCTTCC TCCTCACCTC ATCGCTCTGG ACTCTTCAGT GCCGGGTTTT 
TTCGATGATG TGGGATATCT GGACCTGCTG CCGTCCCGGC CGTGTGACAC CGTCTTCATC 
TTCTACATGA GAGCAGGACA GAAGAGCAGC CAGGAGATCT TGAAGAACGT GGAGTCCTCC 
GCAAACGTGC AGCCACATTT CCTGGAGCTG CTGCTGTCAC TGGGCTGGCC CGTGGAGGTC 
GCTCAGCATC CAGGCTGGAC GGGTAGTGTG TTCACCAGCT GGACCATCAA CACATCCAAC 
GGCGCTGACA CACCAGATGA CTCTGTGACT CTGGGTGACA CTGGCGGTGG TGTGTTTAAC 
GGAGAGAAGC AGGTTCTCTA TTACGCAGAC GCTCTGACAG AAATCGCATT TGTGGTGCCG 
TCTCTCAGCG ACTCCTCTGC GGAGTCATCA GAGCACAGCT TCCCCACCGC AGACTCCGAC 
TCTCAGATGG AGCTGCTGCC CAGTTTACTC AAACAACCCA ACCTCACGCT GGAGCTCTTC 
CCAAACCACT CTGACAACAT GGGCCCTGCG CAACGGTCGC CCACAGCGAA AAACAAGAAG 
TTGCCGCCCG GAAGGACGAT ACCGCCGCTG GGACCCGACA CCAAAGTGTT GGTGGTGTGG 
GTTGAGCGAT ACGACGACAT TGAAAACTTC CCTGTGTCAG AGTTACTAGC AGAGACGAGC 
ACCGGTGTGG AGACGGCCGT CAATAGCAGC GCATCCAGGT CCAGCTCCTC AGAGAAGGAC 
GTCCCCATAG TGTTCATCCA TCCACTGAAA ACCGGCCTGT TCCGCATCAA ACTGCAGGGC 
GCTCTGGGAA AATTCAACAT GGTCATTCCT CTGGTGGACA ACATGGTGGT CAGCAGGAGA 
TCACTGGGTT TTCTGGTGCG TCAGACTGTA ATCAACGCGT GTCGGCGGAA GCGTCTGGAG 
AGCGACTCCT ACAGTCCTCC ACACGTGCGG CGGAAACAGA AGATCGCAGA AATCGTGAAC 
CGTTACCGAA ACAAGCAGCT GGAGCCCGAG TTTTACACCT CGCTCTTCCT GGAGGTCAAC 
GAGACCAGCC TCAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301041.1
CDS	209..4669
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302766.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302766.3

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGACACTGCG GCCGGGCTGT TGATCCGCAG CATTCACCTG 
CTCACCCAGA GACTCAACTC TCAGTGGAGA CTCGACATGA GCATCTCTCT GGCGGCGCTC 
GAGCTGCTCG CGGGACTCGC CAAGGTGAAG GTGAGCGTGG AGTCGTCTGA CCGCAAGCGT 
GCCGTCAGCT CGGTCTGCAG TTATATTGTG TATCAATGCA GTCGTCCAGC TCCGCTTCAT 
TCCAGAGATC TGCACTCCAT GATCGTCGCT GCTTTCCACT GTCTGTGTGT GTGGCTTACT 
GAACACCCCG ACATGCTCAA TGAGAAGGAT TGTCTGATTG AAGTGTTGGA GATCGTGGAG 
CTCGGCATCT CCGGCAGCAA GTCCAAAACA GGCGAACAGG AAGTGAAGTA TAAAGGAGAC 
AAAGAGCACA ACCCTGCATC TATGAGGGTC AAAGATGCCG CAGAGGCCAC GCTATCATGT 
ATAATGCAGG TTTTGGGCGC GTTCCCATCT CCCAGCGGTC CCGCATCGCC CTGCAGTCTG 
CTGAACGAGG ACACGCTCAT CAAATACTCC AGACTCACCT CCACTTCTCA CAGCAACTTC 
AGATACTTCG TCCTGGATAA CTCCGTCATC CTCGCCATGC TGGAGCAGCC GCTGGGGAAC 
GAACAGAATC CCTGTCCGTC TGTCACCATG TTAATTAGAG GTATGTCTGG CCGACATGCA 
TGGACTATGC AGCTTTTCCA TCAGCCTCGA GGAGCAAGAG CCAATCAGAA GCAAGTGTTT 
GTTCCTGAAA GTCGACCCAC TCCCAAAAAT GATGTTGGGA TTCGGTTTAA CGTCAAGCAC 
AGGCCGTTCC CAGAAGAAGT AGATAAGATT CCCTTTGTGA AAGCCGACCT GAGCATCCCA 
GACCTCCATG ACATTGTGGA CAAAGAGCTG GAGCAGCAGC ATGATAAGCT GCGAGTGGTG 
ATGGGGAAGC AGATCGATTA CGAGACCAAA CTAGAGCACC ATACTGAGGA CCTGTGGAGG 
AATAAAGCCT TCCCAGACCC ACTGACCGAC TGTAAACCGC CAGCTCCTGC TCAGGAGTTC 
CAGACTGCTC GCCTTTTCCT GTCACACTTT GGCTTCCTGT CACTAGAGGC GCTCAAGGAG 
CCTGGAAACA GTCGTCTTCC TCCTCACCTC ATCGCTCTGG ACTCTTCAGT GCCGGGTTTT 
TTCGATGATG TGGGATATCT GGACCTGCTG CCGTCCCGGC CGTGTGACAC CGTCTTCATC 
TTCTACATGA GAGCAGGACA GAAGAGCAGC CAGGAGATCT TGAAGAACGT GGAGTCCTCC 
GCAAACGTGC AGCCACATTT CCTGGAGCTG CTGCTGTCAC TGGGCTGGCC CGTGGAGGTC 
GCTCAGCATC CAGGCTGGAC GGGTAGTGTG TTCACCAGCT GGACCATCAA CACATCCAAC 
GGCGCTGACA CACCAGATGA CTCTGTGACT CTGGGTGACA CTGGCGGTGG TGTGTTTAAC 
GGAGAGAAGC AGGTTCTCTA TTACGCAGAC GCTCTGACAG AAATCGCATT TGTGGTGCCG 
TCTCTCAGCG ACTCCTCTGC GGAGTCATCA GAGCACAGCT TCCCCACCGC AGACTCCGAC 
TCTCAGATGG AGCTGCTGCC CAGTTTACTC AAACAACCCA ACCTCACGCT GGAGCTCTTC 
CCAAACCACT CTGACAACAT GGGCCCTGCG CAACGGTCGC CCACAGCGAA AAACAAGAAG 
TTGCCGCCCG GAAGGACGAT ACCGCCGCTG GGACCCGACA CCAAAGTGTT GGTGGTGTGG 
GTTGAGCGAT ACGACGACAT TGAAAACTTC CCTGTGTCAG AGTTACTAGC AGAGACGAGC 
ACCGGTGTGG AGACGGCCGT CAATAGCAGC GCATCCAGGT CCAGCTCCTC AGAGAAGGAC 
GTCCCCATAG TGTTCATCCA TCCACTGAAA ACCGGCCTGT TCCGCATCAA ACTGCAGGGC 
GCTCTGGGAA AATTCAACAT GGTCATTCCT CTGGTGGACA ACATGGTGGT CAGCAGGAGA 
TCACTGGGTT TTCTGGTGCG TCAGACTGTA ATCAACGCGT GTCGGCGGAA GCGTCTGGAG 
AGCGACTCCT ACAGTCCTCC ACACGTGCGG CGGAAACAGA AGATCGCAGA AATCGTGAAC 
CGTTACCGAA ACAAGCAGCT GGAGCCCGAG TTTTACACCT CGCTCTTCCT GGAGGTCAAC 
GAGACCAGCC TCAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_009302766.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301041.1 Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

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CloneID	ODa43046
Clone ID Related Accession (Same CDS sequence)	XM_009302766.3 , XM_009302766.2
Accession Version	XM_009302766.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ral GTPase-activating protein subunit beta isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGACACTGCG GCCGGGCTGT TGATCCGCAG CATTCACCTG 
CTCACCCAGA GACTCAACTC TCAGTGGAGA CTCGACATGA GCATCTCTCT GGCGGCGCTC 
GAGCTGCTCG CGGGACTCGC CAAGGTGAAG GTGAGCGTGG AGTCGTCTGA CCGCAAGCGT 
GCCGTCAGCT CGGTCTGCAG TTATATTGTG TATCAATGCA GTCGTCCAGC TCCGCTTCAT 
TCCAGAGATC TGCACTCCAT GATCGTCGCT GCTTTCCACT GTCTGTGTGT GTGGCTTACT 
GAACACCCCG ACATGCTCAA TGAGAAGGAT TGTCTGATTG AAGTGTTGGA GATCGTGGAG 
CTCGGCATCT CCGGCAGCAA GTCCAAAACA GGCGAACAGG AAGTGAAGTA TAAAGGAGAC 
AAAGAGCACA ACCCTGCATC TATGAGGGTC AAAGATGCCG CAGAGGCCAC GCTATCATGT 
ATAATGCAGG TTTTGGGCGC GTTCCCATCT CCCAGCGGTC CCGCATCGCC CTGCAGTCTG 
CTGAACGAGG ACACGCTCAT CAAATACTCC AGACTCACCT CCACTTCTCA CAGCAACTTC 
AGATACTTCG TCCTGGATAA CTCCGTCATC CTCGCCATGC TGGAGCAGCC GCTGGGGAAC 
GAACAGAATC CCTGTCCGTC TGTCACCATG TTAATTAGAG GTATGTCTGG CCGACATGCA 
TGGACTATGC AGCTTTTCCA TCAGCCTCGA GGAGCAAGAG CCAATCAGAA GCAAGTGTTT 
GTTCCTGAAA GTCGACCCAC TCCCAAAAAT GATGTTGGGA TTCGGTTTAA CGTCAAGCAC 
AGGCCGTTCC CAGAAGAAGT AGATAAGATT CCCTTTGTGA AAGCCGACCT GAGCATCCCA 
GACCTCCATG ACATTGTGGA CAAAGAGCTG GAGCAGCAGC ATGATAAGCT GCGAGTGGTG 
ATGGGGAAGC AGATCGATTA CGAGACCAAA CTAGAGCACC ATACTGAGGA CCTGTGGAGG 
AATAAAGCCT TCCCAGACCC ACTGACCGAC TGTAAACCGC CAGCTCCTGC TCAGGAGTTC 
CAGACTGCTC GCCTTTTCCT GTCACACTTT GGCTTCCTGT CACTAGAGGC GCTCAAGGAG 
CCTGGAAACA GTCGTCTTCC TCCTCACCTC ATCGCTCTGG ACTCTTCAGT GCCGGGTTTT 
TTCGATGATG TGGGATATCT GGACCTGCTG CCGTCCCGGC CGTGTGACAC CGTCTTCATC 
TTCTACATGA GAGCAGGACA GAAGAGCAGC CAGGAGATCT TGAAGAACGT GGAGTCCTCC 
GCAAACGTGC AGCCACATTT CCTGGAGCTG CTGCTGTCAC TGGGCTGGCC CGTGGAGGTC 
GCTCAGCATC CAGGCTGGAC GGGTAGTGTG TTCACCAGCT GGACCATCAA CACATCCAAC 
GGCGCTGACA CACCAGATGA CTCTGTGACT CTGGGTGACA CTGGCGGTGG TGTGTTTAAC 
GGAGAGAAGC AGGTTCTCTA TTACGCAGAC GCTCTGACAG AAATCGCATT TGTGGTGCCG 
TCTCTCAGCG ACTCCTCTGC GGAGTCATCA GAGCACAGCT TCCCCACCGC AGACTCCGAC 
TCTCAGATGG AGCTGCTGCC CAGTTTACTC AAACAACCCA ACCTCACGCT GGAGCTCTTC 
CCAAACCACT CTGACAACAT GGGCCCTGCG CAACGGTCGC CCACAGCGAA AAACAAGAAG 
TTGCCGCCCG GAAGGACGAT ACCGCCGCTG GGACCCGACA CCAAAGTGTT GGTGGTGTGG 
GTTGAGCGAT ACGACGACAT TGAAAACTTC CCTGTGTCAG AGTTACTAGC AGAGACGAGC 
ACCGGTGTGG AGACGGCCGT CAATAGCAGC GCATCCAGGT CCAGCTCCTC AGAGAAGGAC 
GTCCCCATAG TGTTCATCCA TCCACTGAAA ACCGGCCTGT TCCGCATCAA ACTGCAGGGC 
GCTCTGGGAA AATTCAACAT GGTCATTCCT CTGGTGGACA ACATGGTGGT CAGCAGGAGA 
TCACTGGGTT TTCTGGTGCG TCAGACTGTA ATCAACGCGT GTCGGCGGAA GCGTCTGGAG 
AGCGACTCCT ACAGTCCTCC ACACGTGCGG CGGAAACAGA AGATCGCAGA AATCGTGAAC 
CGTTACCGAA ACAAGCAGCT GGAGCCCGAG TTTTACACCT CGCTCTTCCT GGAGGTCAAC 
GAGACCAGCC TCAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301041.1
CDS	185..4645
Translation	MYSEWRSLHLVVQSDQGHLSVLHTYPAGVGRDVANAVVRPLGNSLGGVPSECLLKTDKEVKWTMEVLCYGLTLPLDGDTVKLCVDVYTDWIMALVSPRDSIPQPIIKEPDLYVQTILKHLHNLFLPRPENFSLVHFKLCQQVLSAVQKLARDSSSMARETWEVLLLFLLRINDTLLAPPTVGGGLAEKLAEKLISVLFEVWFLACTRCFPTPPYWKTAREMLANWRHHPPVVEQWSKVIAALTSRLLRFMYGPSFPPFKVPEEDASLIPAEMDNDCVAQTWYRFLHMLSNPVDLSNPVIVSSTPKFQEQLLGVNSVPHEVIQHPCLKQLPQIFFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPPPPTTTNTTPPHNRRHRPTAVAKTTSKSSTTGQTRVSHQPSSTSPLSSPNQTSSEPRPLPAPTRPKVNSVLNLFGQWLFDAALVHCKLHSGLSRDSSMTALATQAGVELRRKGSQMSTDTMVSNPMFDANEFPDNYESGRAEACGTLCRIFCSKKTGEEILPVYLSRFYMVLIQGLQISDFICRPVLASIILNSSALFCSDLKGINVVVPYFISALETILPDRELSKFKSYVNPTELRRASIHILLSMLPLPHHFGNVKSEVLLEGKFSNDDNSLHDKAVTFLSLKLRLVNILIGALQTETDPINTQMILAAMLNIVQDSALLESVGAQAEVGSVDGSHSLAKSHSRNNSGISNASAGSSEATTPDSERPAQALLRDYDTAAGLLIRSIHLLTQRLNSQWRLDMSISLAALELLAGLAKVKVSVESSDRKRAVSSVCSYIVYQCSRPAPLHSRDLHSMIVAAFHCLCVWLTEHPDMLNEKDCLIEVLEIVELGISGSKSKTGEQEVKYKGDKEHNPASMRVKDAAEATLSCIMQVLGAFPSPSGPASPCSLLNEDTLIKYSRLTSTSHSNFRYFVLDNSVILAMLEQPLGNEQNPCPSVTMLIRGMSGRHAWTMQLFHQPRGARANQKQVFVPESRPTPKNDVGIRFNVKHRPFPEEVDKIPFVKADLSIPDLHDIVDKELEQQHDKLRVVMGKQIDYETKLEHHTEDLWRNKAFPDPLTDCKPPAPAQEFQTARLFLSHFGFLSLEALKEPGNSRLPPHLIALDSSVPGFFDDVGYLDLLPSRPCDTVFIFYMRAGQKSSQEILKNVESSANVQPHFLELLLSLGWPVEVAQHPGWTGSVFTSWTINTSNGADTPDDSVTLGDTGGGVFNGEKQVLYYADALTEIAFVVPSLSDSSAESSEHSFPTADSDSQMELLPSLLKQPNLTLELFPNHSDNMGPAQRSPTAKNKKLPPGRTIPPLGPDTKVLVVWVERYDDIENFPVSELLAETSTGVETAVNSSASRSSSSEKDVPIVFIHPLKTGLFRIKLQGALGKFNMVIPLVDNMVVSRRSLGFLVRQTVINACRRKRLESDSYSPPHVRRKQKIAEIVNRYRNKQLEPEFYTSLFLEVNETSLKP

Target ORF information:

RefSeq Version	XM_009302766.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302766.2

ORF Insert Sequence:

ATGTACTCTG AGTGGCGGTC TTTACACCTG GTGGTCCAGA GCGATCAGGG TCACCTGAGT 
GTTTTGCACA CTTACCCAGC TGGTGTGGGT CGAGACGTGG CAAACGCTGT GGTTCGGCCC 
CTGGGAAACA GCCTTGGAGG AGTGCCGTCT GAGTGCCTTC TCAAGACTGA CAAGGAGGTG 
AAATGGACCA TGGAGGTGCT GTGTTACGGC CTGACGCTCC CTCTGGACGG AGACACAGTT 
AAGTTGTGTG TGGACGTTTA CACTGACTGG ATCATGGCAC TAGTGTCTCC AAGAGACTCC 
ATACCACAGC CTATAATCAA AGAGCCCGAC CTCTACGTCC AAACCATCCT CAAACACTTG 
CACAACCTAT TCCTTCCCAG ACCAGAGAAC TTCAGCCTGG TGCACTTTAA ACTGTGTCAG 
CAAGTGTTGT CGGCGGTGCA GAAACTAGCG CGAGACTCAT CCAGCATGGC CAGAGAGACC 
TGGGAGGTGC TGCTGCTCTT CCTGCTGCGC ATCAATGACA CGCTTCTGGC CCCGCCCACT 
GTGGGAGGAG GTCTAGCTGA GAAGCTGGCA GAGAAGTTGA TCAGTGTGCT GTTTGAGGTT 
TGGTTTCTGG CCTGCACACG CTGTTTCCCC ACGCCGCCCT ACTGGAAAAC CGCCCGAGAG 
ATGCTGGCCA ACTGGAGGCA TCATCCTCCG GTGGTGGAGC AGTGGAGTAA AGTCATCGCC 
GCGCTCACGT CCAGGCTGCT GCGGTTCATG TACGGTCCGT CGTTTCCTCC GTTTAAAGTG 
CCAGAAGAGG ACGCCAGTCT CATCCCAGCA GAGATGGACA ATGACTGCGT GGCTCAGACG 
TGGTATCGCT TTCTACACAT GCTCAGTAAT CCTGTGGACC TGAGCAATCC CGTCATTGTG 
AGTTCCACGC CGAAGTTTCA GGAGCAGTTG TTGGGTGTGA ACAGTGTTCC TCACGAGGTG 
ATCCAGCACC CTTGCCTCAA ACAGCTGCCT CAGATCTTCT TCAGAGCCAT GAGGGGCATC 
AGCTGCCTCG TCGACGCTTT CCTCGGTATA TCCCGCCCCC GCTCTGACAG TGCTCCGCCC 
ACTCCTGTCA ATAGGCTCAG TATGCCACCG CCCCCAACAA CAACCAACAC AACACCGCCT 
CACAACCGCA GGCACCGCCC CACTGCTGTT GCCAAGACAA CCAGCAAATC CTCAACGACT 
GGGCAGACCA GAGTATCGCA TCAGCCTTCG TCCACATCTC CTCTCTCCAG CCCAAACCAG 
ACGAGCTCCG AGCCCCGTCC GCTCCCGGCT CCGACCCGGC CCAAAGTCAA CAGCGTGTTA 
AATCTGTTCG GCCAGTGGCT GTTTGACGCT GCGCTGGTCC ACTGTAAACT GCACTCCGGC 
CTGAGCAGAG ACAGCAGCAT GACTGCACTG GCCACTCAAG CTGGAGTTGA ACTGAGGCGT 
AAGGGCTCTC AGATGTCCAC AGACACCATG GTCTCCAACC CCATGTTTGA CGCCAATGAA 
TTTCCTGATA ATTATGAGTC GGGTCGAGCT GAAGCATGCG GGACGCTCTG CAGGATCTTC 
TGCAGTAAGA AAACAGGAGA GGAGATTCTT CCCGTCTACC TGTCCAGGTT CTACATGGTG 
CTGATCCAGG GTCTTCAGAT TTCAGACTTC ATCTGCAGGC CGGTCTTAGC CAGCATTATC 
CTGAACTCTT CTGCTCTGTT CTGCTCCGAT CTCAAGGGAA TCAATGTAGT GGTTCCCTAC 
TTCATATCTG CACTGGAGAC CATCCTGCCG GACCGAGAGC TGTCGAAGTT TAAGTCTTAT 
GTGAATCCTA CTGAGCTGAG AAGAGCCTCC ATCCACATCC TGCTGTCCAT GCTTCCGCTT 
CCTCACCACT TCGGCAACGT CAAATCAGAG GTTCTGTTGG AAGGGAAATT CAGCAATGAT 
GACAACTCTC TCCACGACAA GGCGGTCACC TTCCTCTCCC TGAAGCTGCG ATTGGTCAAT 
ATTCTGATCG GCGCCCTGCA AACAGAGACA GACCCGATCA ATACACAGAT GATACTGGCG 
GCGATGCTGA ATATCGTGCA GGATTCTGCT CTGCTGGAGT CTGTTGGTGC TCAGGCTGAA 
GTGGGCAGTG TGGATGGCAG CCATTCTCTG GCTAAGAGTC ACAGTCGTAA TAACAGTGGC 
ATCAGCAATG CTAGCGCAGG CAGTTCAGAA GCCACCACTC CAGACAGCGA ACGCCCGGCA 
CAGGCCCTGC TGAGAGACTA CGACACTGCG GCCGGGCTGT TGATCCGCAG CATTCACCTG 
CTCACCCAGA GACTCAACTC TCAGTGGAGA CTCGACATGA GCATCTCTCT GGCGGCGCTC 
GAGCTGCTCG CGGGACTCGC CAAGGTGAAG GTGAGCGTGG AGTCGTCTGA CCGCAAGCGT 
GCCGTCAGCT CGGTCTGCAG TTATATTGTG TATCAATGCA GTCGTCCAGC TCCGCTTCAT 
TCCAGAGATC TGCACTCCAT GATCGTCGCT GCTTTCCACT GTCTGTGTGT GTGGCTTACT 
GAACACCCCG ACATGCTCAA TGAGAAGGAT TGTCTGATTG AAGTGTTGGA GATCGTGGAG 
CTCGGCATCT CCGGCAGCAA GTCCAAAACA GGCGAACAGG AAGTGAAGTA TAAAGGAGAC 
AAAGAGCACA ACCCTGCATC TATGAGGGTC AAAGATGCCG CAGAGGCCAC GCTATCATGT 
ATAATGCAGG TTTTGGGCGC GTTCCCATCT CCCAGCGGTC CCGCATCGCC CTGCAGTCTG 
CTGAACGAGG ACACGCTCAT CAAATACTCC AGACTCACCT CCACTTCTCA CAGCAACTTC 
AGATACTTCG TCCTGGATAA CTCCGTCATC CTCGCCATGC TGGAGCAGCC GCTGGGGAAC 
GAACAGAATC CCTGTCCGTC TGTCACCATG TTAATTAGAG GTATGTCTGG CCGACATGCA 
TGGACTATGC AGCTTTTCCA TCAGCCTCGA GGAGCAAGAG CCAATCAGAA GCAAGTGTTT 
GTTCCTGAAA GTCGACCCAC TCCCAAAAAT GATGTTGGGA TTCGGTTTAA CGTCAAGCAC 
AGGCCGTTCC CAGAAGAAGT AGATAAGATT CCCTTTGTGA AAGCCGACCT GAGCATCCCA 
GACCTCCATG ACATTGTGGA CAAAGAGCTG GAGCAGCAGC ATGATAAGCT GCGAGTGGTG 
ATGGGGAAGC AGATCGATTA CGAGACCAAA CTAGAGCACC ATACTGAGGA CCTGTGGAGG 
AATAAAGCCT TCCCAGACCC ACTGACCGAC TGTAAACCGC CAGCTCCTGC TCAGGAGTTC 
CAGACTGCTC GCCTTTTCCT GTCACACTTT GGCTTCCTGT CACTAGAGGC GCTCAAGGAG 
CCTGGAAACA GTCGTCTTCC TCCTCACCTC ATCGCTCTGG ACTCTTCAGT GCCGGGTTTT 
TTCGATGATG TGGGATATCT GGACCTGCTG CCGTCCCGGC CGTGTGACAC CGTCTTCATC 
TTCTACATGA GAGCAGGACA GAAGAGCAGC CAGGAGATCT TGAAGAACGT GGAGTCCTCC 
GCAAACGTGC AGCCACATTT CCTGGAGCTG CTGCTGTCAC TGGGCTGGCC CGTGGAGGTC 
GCTCAGCATC CAGGCTGGAC GGGTAGTGTG TTCACCAGCT GGACCATCAA CACATCCAAC 
GGCGCTGACA CACCAGATGA CTCTGTGACT CTGGGTGACA CTGGCGGTGG TGTGTTTAAC 
GGAGAGAAGC AGGTTCTCTA TTACGCAGAC GCTCTGACAG AAATCGCATT TGTGGTGCCG 
TCTCTCAGCG ACTCCTCTGC GGAGTCATCA GAGCACAGCT TCCCCACCGC AGACTCCGAC 
TCTCAGATGG AGCTGCTGCC CAGTTTACTC AAACAACCCA ACCTCACGCT GGAGCTCTTC 
CCAAACCACT CTGACAACAT GGGCCCTGCG CAACGGTCGC CCACAGCGAA AAACAAGAAG 
TTGCCGCCCG GAAGGACGAT ACCGCCGCTG GGACCCGACA CCAAAGTGTT GGTGGTGTGG 
GTTGAGCGAT ACGACGACAT TGAAAACTTC CCTGTGTCAG AGTTACTAGC AGAGACGAGC 
ACCGGTGTGG AGACGGCCGT CAATAGCAGC GCATCCAGGT CCAGCTCCTC AGAGAAGGAC 
GTCCCCATAG TGTTCATCCA TCCACTGAAA ACCGGCCTGT TCCGCATCAA ACTGCAGGGC 
GCTCTGGGAA AATTCAACAT GGTCATTCCT CTGGTGGACA ACATGGTGGT CAGCAGGAGA 
TCACTGGGTT TTCTGGTGCG TCAGACTGTA ATCAACGCGT GTCGGCGGAA GCGTCTGGAG 
AGCGACTCCT ACAGTCCTCC ACACGTGCGG CGGAAACAGA AGATCGCAGA AATCGTGAAC 
CGTTACCGAA ACAAGCAGCT GGAGCCCGAG TTTTACACCT CGCTCTTCCT GGAGGTCAAC 
GAGACCAGCC TCAAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ralgapb
Entrez Gene ID	559303
Full Name	Ral GTPase activating protein, beta subunit (non-catalytic)
General protein information	Preferred Names Ral GTPase activating protein, beta subunit (non-catalytic) Names ral GTPase-activating protein subunit beta zmp:0000000942
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	RALGAPB	XP_003316982.1
Bos taurus (cattle)	RALGAPB	XP_003586895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007925	XP_317557.4
	RALGAPB	XP_001094087.2
Mus musculus (house mouse)	Ralgapb	NP_808326.2
Gallus gallus (chicken)	RALGAPB	NP_001026017.1
Drosophila melanogaster (fruit fly)	CG34408	NP_572613.2
Homo sapiens (human)	RALGAPB	NP_065069.1
Canis lupus familiaris (dog)	RALGAPB	XP_005635111.1
Rattus norvegicus (Norway rat)	Ralgapb	NP_001258139.1
Danio rerio (zebrafish)	ralgapb	XP_002662611.2
Caenorhabditis elegans (roundworm)	D2085.5	NP_001022054.1
Xenopus tropicalis (tropical clawed frog)	ralgapb	XP_002932905.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ralgapb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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