gcfc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gcfc2
Entrez Gene ID	559280
Full Name	GC-rich sequence DNA-binding factor 2
Synonyms	zgc:171819
General protein information	Preferred Names GC-rich sequence DNA-binding factor 2 Names GC-rich sequence DNA-binding factor 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110107.2	NP_001103577.2	GC-rich sequence DNA-binding factor 2
XM_005158731.3	XP_005158788.1	GC-rich sequence DNA-binding factor 2 isoform X1
XM_005158732.3	XP_005158789.1	GC-rich sequence DNA-binding factor 2 isoform X2
XM_005158731.4	XP_005158788.1	GC-rich sequence DNA-binding factor 2 isoform X1
XM_005158732.4	XP_005158789.1	GC-rich sequence DNA-binding factor 2 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC101734739	XP_004918235.1
	C2orf3	XP_001106597.2
Mus musculus (house mouse)	Gcfc2	NP_808552.2
Danio rerio (zebrafish)	gcfc2	NP_001103577.2
Xenopus tropicalis (tropical clawed frog)	gcfc2	XP_002936033.2
Homo sapiens (human)	GCFC2	NP_003194.3
Pan troglodytes (chimpanzee)	GCFC2	XP_001145277.2
Bos taurus (cattle)	GCFC2	XP_003586682.1
Canis lupus familiaris (dog)	GCFC2	XP_540209.3
Rattus norvegicus (Norway rat)	Gcfc2	NP_001128026.1

Gene Ontology
Bibliography

Related articles in PubMed

Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish.
Mishima Y, Tomari Y
Genes to cells : devoted to molecular & cellular mechanisms22(7)670-678(2017 Jul)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gcfc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcfc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35456	NM_001110107.2 Latest version!	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa22689	XM_005158731.4 Latest version!	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa22690	XM_005158732.4 Latest version!	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa22689	XM_005158731.3	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa22690	XM_005158732.3	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcfc2 ( NM_001110107.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103577.2 Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Price & Availability↑

CloneID	ODa35456
Clone ID Related Accession (Same CDS sequence)	NM_001110107.2
Accession Version	NM_001110107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	GC-rich sequence DNA-binding factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR352331.8. On Jan 25, 2011 this sequence version replaced NM_001110107.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01011278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
AAGGAATGGC ATGCTCCGAA AGCAGCCCGA CAGAAGAGAG TGGAAATCCC AGAAACGCTT 
CCAGTAGTTA GTATTGGTGT CATTAAGAAG AGAATCACTG GAAAATTGGA TTCGCTGAGG 
GAGGTACACA GGGCACGGGA GGCGCAGCTA AGGAGGATGC AGCTGGATGT GGAAATGGCC 
AAAACCTCTT TAGAGGGCCT TGAAAACAAC AATGCAGATG AGCAGCTCCG ATTTTACCGC 
ACCATGAACG TTTTCTCACA GAACCTGCTG GAATGCCTAT CTGAGAAGAT GGCTTTAATA 
AACTCAGTTG AGCTGGACAT GCACGGTCTC TATATTGACC AGGCGGAGGC ACTCTTGAGT 
CAGAGAAGAG AAGCATTACA AGAGGAGTCC TCTCGGATTC AGAAGTTAAC TTATAACACT 
GATTCACACA GCAATGGTGA CACAGCCGGA GACTGCAGCC CTACTCAGCA GAGCTTGTCT 
GAAGATGACA TTGGATGTGT GCCGTGTGAC TGGGAACCAA CGGTCGAGCA GAAGGAGGAG 
ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA AAGGCACAGG AAGTGTTTGC TGATGTTCAA 
AATGAGTTCT GGGATGTAAA GAAAATCCTC TCCAGATTTG ATGAGTGGCG CGTCTCCTTT 
AAAGACTCTT ACAATAATGC CTACATTGGG CTGTGTCTCC CAAAACTCCT GGCACCCTTG 
ATCCGACACC AGCTCATTGG CTGGAACCCT CTACAGGCAG AAAGTGAGGA CTTTGAGGCA 
TTGCCATGGT ATTGTGCTGT TGAGAGGTTC TGTCATGGTC AGGGCTACGA GGAATCAGAG 
AACATGGACA AGACCACACT GCCCACCATA ATTGAGAAGA CCATCCTCTC TAAAGTTCAA 
GGTTTTGTAG AGCTGGTCTG GGACCCTCTT TCTGCCCAGC AGACTCGAAC CCTGACAACA 
CTATGCAGAA GAATACAGCA TGACTATTCT GTGTTTAATG GCGAACAAAG CAAACCTGTC 
AAGGCCTTTG TGGAAGCAGT CATTCAGAGA TTAAGGACTG CCGTAGACGA TGATGTCTTT 
GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG GATAAGCGGT CACCGCAGTT CCAGTTTCAG 
AACAAACAGT TTTGGTCGGC GGTGAAGTTG CTAGGTAATA TGGCATTATG GGGTGGTTTG 
ATTCCTGAGC ACATTCTTAA AGAGCTGATG TTGGAGAAAC TTCTGGGCCG TTATTTGATG 
ATAACCATTC TTAATGAATC TGATCCAAAA CACACAGTCC AGAAATGCAA AAAGATTGCA 
GGTTGTTTTC CAGAATCATG GTTTATTGAT TTAAATACTG GATCATCACT ACCTCAGTTG 
CAAAACTTCA GCAAACATCT TCTTCAAACC GCACATCTGA TATTCAAGGA CAACAAGGAC 
TCCAGAGGAC TTTTATCTGA TGTGCTGTTT GTTCTCAAGA TCATCAAGGC TCATGACAGT 
ATTAGGACTA TCACTGAAAA ATATAATTGC AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103577.2
CDS	52..2445
Translation	MFNKKPRRNFRQRKNESSDEDDNGKLAAGEGGKDADLKADLPSKAGISSSSREARECDSSEAEEEASVSALQTKDRKLHTDSKTLSFSDDKDSTEGGFQVKRSVNREVLFQARKKEDSPAPATSSQGKKSKDVSLSDSDSDASAEDIPPDSDEETCSTMSSSSASSLPQRVIIPDARKIHAAKEKRRQARAQQDYISLDSPRTPGSLKEDELSDKEQDSDNDLDDHERRIEFAPKPKTLRERMAEKMGSDSEESFSDSQEEEEQQMWEEQQIGKGVKRHQKEWHAPKAARQKRVEIPETLPVVSIGVIKKRITGKLDSLREVHRAREAQLRRMQLDVEMAKTSLEGLENNNADEQLRFYRTMNVFSQNLLECLSEKMALINSVELDMHGLYIDQAEALLSQRREALQEESSRIQKLTYNTDSHSNGDTAGDCSPTQQSLSEDDIGCVPCDWEPTVEQKEEIESKRAALLKKAQEVFADVQNEFWDVKKILSRFDEWRVSFKDSYNNAYIGLCLPKLLAPLIRHQLIGWNPLQAESEDFEALPWYCAVERFCHGQGYEESENMDKTTLPTIIEKTILSKVQGFVELVWDPLSAQQTRTLTTLCRRIQHDYSVFNGEQSKPVKAFVEAVIQRLRTAVDDDVFVPLYPKKFLEDKRSPQFQFQNKQFWSAVKLLGNMALWGGLIPEHILKELMLEKLLGRYLMITILNESDPKHTVQKCKKIAGCFPESWFIDLNTGSSLPQLQNFSKHLLQTAHLIFKDNKDSRGLLSDVLFVLKIIKAHDSIRTITEKYNCKDLLKAL

Target ORF information:

RefSeq Version	NM_001110107.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110107.2

ORF Insert Sequence:

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
AAGGAATGGC ATGCTCCGAA AGCAGCCCGA CAGAAGAGAG TGGAAATCCC AGAAACGCTT 
CCAGTAGTTA GTATTGGTGT CATTAAGAAG AGAATCACTG GAAAATTGGA TTCGCTGAGG 
GAGGTACACA GGGCACGGGA GGCGCAGCTA AGGAGGATGC AGCTGGATGT GGAAATGGCC 
AAAACCTCTT TAGAGGGCCT TGAAAACAAC AATGCAGATG AGCAGCTCCG ATTTTACCGC 
ACCATGAACG TTTTCTCACA GAACCTGCTG GAATGCCTAT CTGAGAAGAT GGCTTTAATA 
AACTCAGTTG AGCTGGACAT GCACGGTCTC TATATTGACC AGGCGGAGGC ACTCTTGAGT 
CAGAGAAGAG AAGCATTACA AGAGGAGTCC TCTCGGATTC AGAAGTTAAC TTATAACACT 
GATTCACACA GCAATGGTGA CACAGCCGGA GACTGCAGCC CTACTCAGCA GAGCTTGTCT 
GAAGATGACA TTGGATGTGT GCCGTGTGAC TGGGAACCAA CGGTCGAGCA GAAGGAGGAG 
ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA AAGGCACAGG AAGTGTTTGC TGATGTTCAA 
AATGAGTTCT GGGATGTAAA GAAAATCCTC TCCAGATTTG ATGAGTGGCG CGTCTCCTTT 
AAAGACTCTT ACAATAATGC CTACATTGGG CTGTGTCTCC CAAAACTCCT GGCACCCTTG 
ATCCGACACC AGCTCATTGG CTGGAACCCT CTACAGGCAG AAAGTGAGGA CTTTGAGGCA 
TTGCCATGGT ATTGTGCTGT TGAGAGGTTC TGTCATGGTC AGGGCTACGA GGAATCAGAG 
AACATGGACA AGACCACACT GCCCACCATA ATTGAGAAGA CCATCCTCTC TAAAGTTCAA 
GGTTTTGTAG AGCTGGTCTG GGACCCTCTT TCTGCCCAGC AGACTCGAAC CCTGACAACA 
CTATGCAGAA GAATACAGCA TGACTATTCT GTGTTTAATG GCGAACAAAG CAAACCTGTC 
AAGGCCTTTG TGGAAGCAGT CATTCAGAGA TTAAGGACTG CCGTAGACGA TGATGTCTTT 
GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG GATAAGCGGT CACCGCAGTT CCAGTTTCAG 
AACAAACAGT TTTGGTCGGC GGTGAAGTTG CTAGGTAATA TGGCATTATG GGGTGGTTTG 
ATTCCTGAGC ACATTCTTAA AGAGCTGATG TTGGAGAAAC TTCTGGGCCG TTATTTGATG 
ATAACCATTC TTAATGAATC TGATCCAAAA CACACAGTCC AGAAATGCAA AAAGATTGCA 
GGTTGTTTTC CAGAATCATG GTTTATTGAT TTAAATACTG GATCATCACT ACCTCAGTTG 
CAAAACTTCA GCAAACATCT TCTTCAAACC GCACATCTGA TATTCAAGGA CAACAAGGAC 
TCCAGAGGAC TTTTATCTGA TGTGCTGTTT GTTCTCAAGA TCATCAAGGC TCATGACAGT 
ATTAGGACTA TCACTGAAAA ATATAATTGC AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcfc2 ( XM_005158731.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158788.1 Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22689
Clone ID Related Accession (Same CDS sequence)	XM_005158731.3 , XM_005158731.4
Accession Version	XM_005158731.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	GC-rich sequence DNA-binding factor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
TGTTTCAAAG ACCTTTCCTT ATATTCCCAG AAGGAATGGC ATGCTCCGAA AGCAGCCCGA 
CAGAAGAGAG TGGAAATCCC AGAAACGCTT CCAGTAGTTA GTATTGGTGT CATTAAGAAG 
AGAATCACTG GAAAATTGGA TTCGCTGAGG GAGGTACACA GGGCACGGGA GGCGCAGCTA 
AGGAGGATGC AGCTGGATGT GGAAATGGCC AAAACCTCTT TAGAGGGCCT TGAAAACAAC 
AATGCAGATG AGCAGCTCCG ATTTTACCGC ACCATGAACG TTTTCTCACA GAACCTGCTG 
GAATGCCTAT CTGAGAAGAT GGCTTTAATA AACTCAGTTG AGCTGGACAT GCACGGTCTC 
TATATTGACC AGGCGGAGGC ACTCTTGAGT CAGAGAAGAG AAGCATTACA AGAGGAGTCC 
TCTCGGATTC AGAAGTTAAC TTATAACACT GATTCACACA GCAATGGTGA CACAGCCGGA 
GACTGCAGCC CTACTCAGCA GAGCTTGTCT GAAGATGACA TTGGATGTGT GCCGTGTGAC 
TGGGAACCAA CGGTCGAGCA GAAGGAGGAG ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA 
AAGGCACAGG AAGTGTTTGC TGATGTTCAA AATGAGTTCT GGGATGTAAA GAAAATCCTC 
TCCAGATTTG ATGAGTGGCG CGTCTCCTTT AAAGACTCTT ACAATAATGC CTACATTGGG 
CTGTGTCTCC CAAAACTCCT GGCACCCTTG ATCCGACACC AGCTCATTGG CTGGAACCCT 
CTACAGGCAG AAAGTGAGGA CTTTGAGGCA TTGCCATGGT ATTGTGCTGT TGAGAGGTTC 
TGTCATGGTC AGGGCTACGA GGAATCAGAG AACATGGACA AGACCACACT GCCCACCATA 
ATTGAGAAGA CCATCCTCTC TAAAGTTCAA GGTTTTGTAG AGCTGGTCTG GGACCCTCTT 
TCTGCCCAGC AGACTCGAAC CCTGACAACA CTATGCAGAA GAATACAGCA TGACTATTCT 
GTGTTTAATG GCGAACAAAG CAAACCTGTC AAGGCCTTTG TGGAAGCAGT CATTCAGAGA 
TTAAGGACTG CCGTAGACGA TGATGTCTTT GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG 
GATAAGCGGT CACCGCAGTT CCAGTTTCAG AACAAACAGT TTTGGTCGGC GGTGAAGTTG 
CTAGGTAATA TGGCATTATG GGGTGGTTTG ATTCCTGAGC ACATTCTTAA AGAGCTGATG 
TTGGAGAAAC TTCTGGGCCG TTATTTGATG ATAACCATTC TTAATGAATC TGATCCAAAA 
CACACAGTCC AGAAATGCAA AAAGATTGCA GGTTGTTTTC CAGAATCATG GTTTATTGAT 
TTAAATACTG GATCATCACT ACCTCAGTTG CAAAACTTCA GCAAACATCT TCTTCAAACC 
GCACATCTGA TATTCAAGGA CAACAAGGAC TCCAGAGGAC TTTTATCTGA TGTGCTGTTT 
GTTCTCAAGA TCATCAAGGC TCATGACAGT ATTAGGACTA TCACTGAAAA ATATAATTGC 
AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158788.1
CDS	70..2493
Translation	MFNKKPRRNFRQRKNESSDEDDNGKLAAGEGGKDADLKADLPSKAGISSSSREARECDSSEAEEEASVSALQTKDRKLHTDSKTLSFSDDKDSTEGGFQVKRSVNREVLFQARKKEDSPAPATSSQGKKSKDVSLSDSDSDASAEDIPPDSDEETCSTMSSSSASSLPQRVIIPDARKIHAAKEKRRQARAQQDYISLDSPRTPGSLKEDELSDKEQDSDNDLDDHERRIEFAPKPKTLRERMAEKMGSDSEESFSDSQEEEEQQMWEEQQIGKGVKRHQCFKDLSLYSQKEWHAPKAARQKRVEIPETLPVVSIGVIKKRITGKLDSLREVHRAREAQLRRMQLDVEMAKTSLEGLENNNADEQLRFYRTMNVFSQNLLECLSEKMALINSVELDMHGLYIDQAEALLSQRREALQEESSRIQKLTYNTDSHSNGDTAGDCSPTQQSLSEDDIGCVPCDWEPTVEQKEEIESKRAALLKKAQEVFADVQNEFWDVKKILSRFDEWRVSFKDSYNNAYIGLCLPKLLAPLIRHQLIGWNPLQAESEDFEALPWYCAVERFCHGQGYEESENMDKTTLPTIIEKTILSKVQGFVELVWDPLSAQQTRTLTTLCRRIQHDYSVFNGEQSKPVKAFVEAVIQRLRTAVDDDVFVPLYPKKFLEDKRSPQFQFQNKQFWSAVKLLGNMALWGGLIPEHILKELMLEKLLGRYLMITILNESDPKHTVQKCKKIAGCFPESWFIDLNTGSSLPQLQNFSKHLLQTAHLIFKDNKDSRGLLSDVLFVLKIIKAHDSIRTITEKYNCKDLLKAL

Target ORF information:

RefSeq Version	XM_005158731.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158731.3

ORF Insert Sequence:

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
TGTTTCAAAG ACCTTTCCTT ATATTCCCAG AAGGAATGGC ATGCTCCGAA AGCAGCCCGA 
CAGAAGAGAG TGGAAATCCC AGAAACGCTT CCAGTAGTTA GTATTGGTGT CATTAAGAAG 
AGAATCACTG GAAAATTGGA TTCGCTGAGG GAGGTACACA GGGCACGGGA GGCGCAGCTA 
AGGAGGATGC AGCTGGATGT GGAAATGGCC AAAACCTCTT TAGAGGGCCT TGAAAACAAC 
AATGCAGATG AGCAGCTCCG ATTTTACCGC ACCATGAACG TTTTCTCACA GAACCTGCTG 
GAATGCCTAT CTGAGAAGAT GGCTTTAATA AACTCAGTTG AGCTGGACAT GCACGGTCTC 
TATATTGACC AGGCGGAGGC ACTCTTGAGT CAGAGAAGAG AAGCATTACA AGAGGAGTCC 
TCTCGGATTC AGAAGTTAAC TTATAACACT GATTCACACA GCAATGGTGA CACAGCCGGA 
GACTGCAGCC CTACTCAGCA GAGCTTGTCT GAAGATGACA TTGGATGTGT GCCGTGTGAC 
TGGGAACCAA CGGTCGAGCA GAAGGAGGAG ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA 
AAGGCACAGG AAGTGTTTGC TGATGTTCAA AATGAGTTCT GGGATGTAAA GAAAATCCTC 
TCCAGATTTG ATGAGTGGCG CGTCTCCTTT AAAGACTCTT ACAATAATGC CTACATTGGG 
CTGTGTCTCC CAAAACTCCT GGCACCCTTG ATCCGACACC AGCTCATTGG CTGGAACCCT 
CTACAGGCAG AAAGTGAGGA CTTTGAGGCA TTGCCATGGT ATTGTGCTGT TGAGAGGTTC 
TGTCATGGTC AGGGCTACGA GGAATCAGAG AACATGGACA AGACCACACT GCCCACCATA 
ATTGAGAAGA CCATCCTCTC TAAAGTTCAA GGTTTTGTAG AGCTGGTCTG GGACCCTCTT 
TCTGCCCAGC AGACTCGAAC CCTGACAACA CTATGCAGAA GAATACAGCA TGACTATTCT 
GTGTTTAATG GCGAACAAAG CAAACCTGTC AAGGCCTTTG TGGAAGCAGT CATTCAGAGA 
TTAAGGACTG CCGTAGACGA TGATGTCTTT GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG 
GATAAGCGGT CACCGCAGTT CCAGTTTCAG AACAAACAGT TTTGGTCGGC GGTGAAGTTG 
CTAGGTAATA TGGCATTATG GGGTGGTTTG ATTCCTGAGC ACATTCTTAA AGAGCTGATG 
TTGGAGAAAC TTCTGGGCCG TTATTTGATG ATAACCATTC TTAATGAATC TGATCCAAAA 
CACACAGTCC AGAAATGCAA AAAGATTGCA GGTTGTTTTC CAGAATCATG GTTTATTGAT 
TTAAATACTG GATCATCACT ACCTCAGTTG CAAAACTTCA GCAAACATCT TCTTCAAACC 
GCACATCTGA TATTCAAGGA CAACAAGGAC TCCAGAGGAC TTTTATCTGA TGTGCTGTTT 
GTTCTCAAGA TCATCAAGGC TCATGACAGT ATTAGGACTA TCACTGAAAA ATATAATTGC 
AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcfc2 ( XM_005158731.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158788.1 Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22689
Clone ID Related Accession (Same CDS sequence)	XM_005158731.3 , XM_005158731.4
Accession Version	XM_005158731.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GC-rich sequence DNA-binding factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158731.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
TGTTTCAAAG ACCTTTCCTT ATATTCCCAG AAGGAATGGC ATGCTCCGAA AGCAGCCCGA 
CAGAAGAGAG TGGAAATCCC AGAAACGCTT CCAGTAGTTA GTATTGGTGT CATTAAGAAG 
AGAATCACTG GAAAATTGGA TTCGCTGAGG GAGGTACACA GGGCACGGGA GGCGCAGCTA 
AGGAGGATGC AGCTGGATGT GGAAATGGCC AAAACCTCTT TAGAGGGCCT TGAAAACAAC 
AATGCAGATG AGCAGCTCCG ATTTTACCGC ACCATGAACG TTTTCTCACA GAACCTGCTG 
GAATGCCTAT CTGAGAAGAT GGCTTTAATA AACTCAGTTG AGCTGGACAT GCACGGTCTC 
TATATTGACC AGGCGGAGGC ACTCTTGAGT CAGAGAAGAG AAGCATTACA AGAGGAGTCC 
TCTCGGATTC AGAAGTTAAC TTATAACACT GATTCACACA GCAATGGTGA CACAGCCGGA 
GACTGCAGCC CTACTCAGCA GAGCTTGTCT GAAGATGACA TTGGATGTGT GCCGTGTGAC 
TGGGAACCAA CGGTCGAGCA GAAGGAGGAG ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA 
AAGGCACAGG AAGTGTTTGC TGATGTTCAA AATGAGTTCT GGGATGTAAA GAAAATCCTC 
TCCAGATTTG ATGAGTGGCG CGTCTCCTTT AAAGACTCTT ACAATAATGC CTACATTGGG 
CTGTGTCTCC CAAAACTCCT GGCACCCTTG ATCCGACACC AGCTCATTGG CTGGAACCCT 
CTACAGGCAG AAAGTGAGGA CTTTGAGGCA TTGCCATGGT ATTGTGCTGT TGAGAGGTTC 
TGTCATGGTC AGGGCTACGA GGAATCAGAG AACATGGACA AGACCACACT GCCCACCATA 
ATTGAGAAGA CCATCCTCTC TAAAGTTCAA GGTTTTGTAG AGCTGGTCTG GGACCCTCTT 
TCTGCCCAGC AGACTCGAAC CCTGACAACA CTATGCAGAA GAATACAGCA TGACTATTCT 
GTGTTTAATG GCGAACAAAG CAAACCTGTC AAGGCCTTTG TGGAAGCAGT CATTCAGAGA 
TTAAGGACTG CCGTAGACGA TGATGTCTTT GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG 
GATAAGCGGT CACCGCAGTT CCAGTTTCAG AACAAACAGT TTTGGTCGGC GGTGAAGTTG 
CTAGGTAATA TGGCATTATG GGGTGGTTTG ATTCCTGAGC ACATTCTTAA AGAGCTGATG 
TTGGAGAAAC TTCTGGGCCG TTATTTGATG ATAACCATTC TTAATGAATC TGATCCAAAA 
CACACAGTCC AGAAATGCAA AAAGATTGCA GGTTGTTTTC CAGAATCATG GTTTATTGAT 
TTAAATACTG GATCATCACT ACCTCAGTTG CAAAACTTCA GCAAACATCT TCTTCAAACC 
GCACATCTGA TATTCAAGGA CAACAAGGAC TCCAGAGGAC TTTTATCTGA TGTGCTGTTT 
GTTCTCAAGA TCATCAAGGC TCATGACAGT ATTAGGACTA TCACTGAAAA ATATAATTGC 
AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158788.1
CDS	72..2495
Translation	MFNKKPRRNFRQRKNESSDEDDNGKLAAGEGGKDADLKADLPSKAGISSSSREARECDSSEAEEEASVSALQTKDRKLHTDSKTLSFSDDKDSTEGGFQVKRSVNREVLFQARKKEDSPAPATSSQGKKSKDVSLSDSDSDASAEDIPPDSDEETCSTMSSSSASSLPQRVIIPDARKIHAAKEKRRQARAQQDYISLDSPRTPGSLKEDELSDKEQDSDNDLDDHERRIEFAPKPKTLRERMAEKMGSDSEESFSDSQEEEEQQMWEEQQIGKGVKRHQCFKDLSLYSQKEWHAPKAARQKRVEIPETLPVVSIGVIKKRITGKLDSLREVHRAREAQLRRMQLDVEMAKTSLEGLENNNADEQLRFYRTMNVFSQNLLECLSEKMALINSVELDMHGLYIDQAEALLSQRREALQEESSRIQKLTYNTDSHSNGDTAGDCSPTQQSLSEDDIGCVPCDWEPTVEQKEEIESKRAALLKKAQEVFADVQNEFWDVKKILSRFDEWRVSFKDSYNNAYIGLCLPKLLAPLIRHQLIGWNPLQAESEDFEALPWYCAVERFCHGQGYEESENMDKTTLPTIIEKTILSKVQGFVELVWDPLSAQQTRTLTTLCRRIQHDYSVFNGEQSKPVKAFVEAVIQRLRTAVDDDVFVPLYPKKFLEDKRSPQFQFQNKQFWSAVKLLGNMALWGGLIPEHILKELMLEKLLGRYLMITILNESDPKHTVQKCKKIAGCFPESWFIDLNTGSSLPQLQNFSKHLLQTAHLIFKDNKDSRGLLSDVLFVLKIIKAHDSIRTITEKYNCKDLLKAL

Target ORF information:

RefSeq Version	XM_005158731.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158731.4

ORF Insert Sequence:

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
TGTTTCAAAG ACCTTTCCTT ATATTCCCAG AAGGAATGGC ATGCTCCGAA AGCAGCCCGA 
CAGAAGAGAG TGGAAATCCC AGAAACGCTT CCAGTAGTTA GTATTGGTGT CATTAAGAAG 
AGAATCACTG GAAAATTGGA TTCGCTGAGG GAGGTACACA GGGCACGGGA GGCGCAGCTA 
AGGAGGATGC AGCTGGATGT GGAAATGGCC AAAACCTCTT TAGAGGGCCT TGAAAACAAC 
AATGCAGATG AGCAGCTCCG ATTTTACCGC ACCATGAACG TTTTCTCACA GAACCTGCTG 
GAATGCCTAT CTGAGAAGAT GGCTTTAATA AACTCAGTTG AGCTGGACAT GCACGGTCTC 
TATATTGACC AGGCGGAGGC ACTCTTGAGT CAGAGAAGAG AAGCATTACA AGAGGAGTCC 
TCTCGGATTC AGAAGTTAAC TTATAACACT GATTCACACA GCAATGGTGA CACAGCCGGA 
GACTGCAGCC CTACTCAGCA GAGCTTGTCT GAAGATGACA TTGGATGTGT GCCGTGTGAC 
TGGGAACCAA CGGTCGAGCA GAAGGAGGAG ATTGAAAGTA AAAGAGCTGC TCTGCTGAAA 
AAGGCACAGG AAGTGTTTGC TGATGTTCAA AATGAGTTCT GGGATGTAAA GAAAATCCTC 
TCCAGATTTG ATGAGTGGCG CGTCTCCTTT AAAGACTCTT ACAATAATGC CTACATTGGG 
CTGTGTCTCC CAAAACTCCT GGCACCCTTG ATCCGACACC AGCTCATTGG CTGGAACCCT 
CTACAGGCAG AAAGTGAGGA CTTTGAGGCA TTGCCATGGT ATTGTGCTGT TGAGAGGTTC 
TGTCATGGTC AGGGCTACGA GGAATCAGAG AACATGGACA AGACCACACT GCCCACCATA 
ATTGAGAAGA CCATCCTCTC TAAAGTTCAA GGTTTTGTAG AGCTGGTCTG GGACCCTCTT 
TCTGCCCAGC AGACTCGAAC CCTGACAACA CTATGCAGAA GAATACAGCA TGACTATTCT 
GTGTTTAATG GCGAACAAAG CAAACCTGTC AAGGCCTTTG TGGAAGCAGT CATTCAGAGA 
TTAAGGACTG CCGTAGACGA TGATGTCTTT GTCCCTCTGT ACCCCAAAAA GTTTTTAGAG 
GATAAGCGGT CACCGCAGTT CCAGTTTCAG AACAAACAGT TTTGGTCGGC GGTGAAGTTG 
CTAGGTAATA TGGCATTATG GGGTGGTTTG ATTCCTGAGC ACATTCTTAA AGAGCTGATG 
TTGGAGAAAC TTCTGGGCCG TTATTTGATG ATAACCATTC TTAATGAATC TGATCCAAAA 
CACACAGTCC AGAAATGCAA AAAGATTGCA GGTTGTTTTC CAGAATCATG GTTTATTGAT 
TTAAATACTG GATCATCACT ACCTCAGTTG CAAAACTTCA GCAAACATCT TCTTCAAACC 
GCACATCTGA TATTCAAGGA CAACAAGGAC TCCAGAGGAC TTTTATCTGA TGTGCTGTTT 
GTTCTCAAGA TCATCAAGGC TCATGACAGT ATTAGGACTA TCACTGAAAA ATATAATTGC 
AAAGACCTGT TAAAGGCTCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcfc2 ( XM_005158732.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158789.1 Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22690
Clone ID Related Accession (Same CDS sequence)	XM_005158732.3 , XM_005158732.4
Accession Version	XM_005158732.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	GC-rich sequence DNA-binding factor 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158732.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
GAATGGCATG CTCCGAAAGC AGCCCGACAG AAGAGAGTGG AAATCCCAGA AACGCTTCCA 
GTAGTTAGTA TTGGTGTCAT TAAGAAGAGA ATCACTGGAA AATTGGATTC GCTGAGGGAG 
GTACACAGGG CACGGGAGGC GCAGCTAAGG AGGATGCAGC TGGATGTGGA AATGGCCAAA 
ACCTCTTTAG AGGGCCTTGA AAACAACAAT GCAGATGAGC AGCTCCGATT TTACCGCACC 
ATGAACGTTT TCTCACAGAA CCTGCTGGAA TGCCTATCTG AGAAGATGGC TTTAATAAAC 
TCAGTTGAGC TGGACATGCA CGGTCTCTAT ATTGACCAGG CGGAGGCACT CTTGAGTCAG 
AGAAGAGAAG CATTACAAGA GGAGTCCTCT CGGATTCAGA AGTTAACTTA TAACACTGAT 
TCACACAGCA ATGGTGACAC AGCCGGAGAC TGCAGCCCTA CTCAGCAGAG CTTGTCTGAA 
GATGACATTG GATGTGTGCC GTGTGACTGG GAACCAACGG TCGAGCAGAA GGAGGAGATT 
GAAAGTAAAA GAGCTGCTCT GCTGAAAAAG GCACAGGAAG TGTTTGCTGA TGTTCAAAAT 
GAGTTCTGGG ATGTAAAGAA AATCCTCTCC AGATTTGATG AGTGGCGCGT CTCCTTTAAA 
GACTCTTACA ATAATGCCTA CATTGGGCTG TGTCTCCCAA AACTCCTGGC ACCCTTGATC 
CGACACCAGC TCATTGGCTG GAACCCTCTA CAGGCAGAAA GTGAGGACTT TGAGGCATTG 
CCATGGTATT GTGCTGTTGA GAGGTTCTGT CATGGTCAGG GCTACGAGGA ATCAGAGAAC 
ATGGACAAGA CCACACTGCC CACCATAATT GAGAAGACCA TCCTCTCTAA AGTTCAAGGT 
TTTGTAGAGC TGGTCTGGGA CCCTCTTTCT GCCCAGCAGA CTCGAACCCT GACAACACTA 
TGCAGAAGAA TACAGCATGA CTATTCTGTG TTTAATGGCG AACAAAGCAA ACCTGTCAAG 
GCCTTTGTGG AAGCAGTCAT TCAGAGATTA AGGACTGCCG TAGACGATGA TGTCTTTGTC 
CCTCTGTACC CCAAAAAGTT TTTAGAGGAT AAGCGGTCAC CGCAGTTCCA GTTTCAGAAC 
AAACAGTTTT GGTCGGCGGT GAAGTTGCTA GGTAATATGG CATTATGGGG TGGTTTGATT 
CCTGAGCACA TTCTTAAAGA GCTGATGTTG GAGAAACTTC TGGGCCGTTA TTTGATGATA 
ACCATTCTTA ATGAATCTGA TCCAAAACAC ACAGTCCAGA AATGCAAAAA GATTGCAGGT 
TGTTTTCCAG AATCATGGTT TATTGATTTA AATACTGGAT CATCACTACC TCAGTTGCAA 
AACTTCAGCA AACATCTTCT TCAAACCGCA CATCTGATAT TCAAGGACAA CAAGGACTCC 
AGAGGACTTT TATCTGATGT GCTGTTTGTT CTCAAGATCA TCAAGGCTCA TGACAGTATT 
AGGACTATCA CTGAAAAATA TAATTGCAAA GACCTGTTAA AGGCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158789.1
CDS	69..2459
Translation	MFNKKPRRNFRQRKNESSDEDDNGKLAAGEGGKDADLKADLPSKAGISSSSREARECDSSEAEEEASVSALQTKDRKLHTDSKTLSFSDDKDSTEGGFQVKRSVNREVLFQARKKEDSPAPATSSQGKKSKDVSLSDSDSDASAEDIPPDSDEETCSTMSSSSASSLPQRVIIPDARKIHAAKEKRRQARAQQDYISLDSPRTPGSLKEDELSDKEQDSDNDLDDHERRIEFAPKPKTLRERMAEKMGSDSEESFSDSQEEEEQQMWEEQQIGKGVKRHQEWHAPKAARQKRVEIPETLPVVSIGVIKKRITGKLDSLREVHRAREAQLRRMQLDVEMAKTSLEGLENNNADEQLRFYRTMNVFSQNLLECLSEKMALINSVELDMHGLYIDQAEALLSQRREALQEESSRIQKLTYNTDSHSNGDTAGDCSPTQQSLSEDDIGCVPCDWEPTVEQKEEIESKRAALLKKAQEVFADVQNEFWDVKKILSRFDEWRVSFKDSYNNAYIGLCLPKLLAPLIRHQLIGWNPLQAESEDFEALPWYCAVERFCHGQGYEESENMDKTTLPTIIEKTILSKVQGFVELVWDPLSAQQTRTLTTLCRRIQHDYSVFNGEQSKPVKAFVEAVIQRLRTAVDDDVFVPLYPKKFLEDKRSPQFQFQNKQFWSAVKLLGNMALWGGLIPEHILKELMLEKLLGRYLMITILNESDPKHTVQKCKKIAGCFPESWFIDLNTGSSLPQLQNFSKHLLQTAHLIFKDNKDSRGLLSDVLFVLKIIKAHDSIRTITEKYNCKDLLKAL

Target ORF information:

RefSeq Version	XM_005158732.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158732.3

ORF Insert Sequence:

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
GAATGGCATG CTCCGAAAGC AGCCCGACAG AAGAGAGTGG AAATCCCAGA AACGCTTCCA 
GTAGTTAGTA TTGGTGTCAT TAAGAAGAGA ATCACTGGAA AATTGGATTC GCTGAGGGAG 
GTACACAGGG CACGGGAGGC GCAGCTAAGG AGGATGCAGC TGGATGTGGA AATGGCCAAA 
ACCTCTTTAG AGGGCCTTGA AAACAACAAT GCAGATGAGC AGCTCCGATT TTACCGCACC 
ATGAACGTTT TCTCACAGAA CCTGCTGGAA TGCCTATCTG AGAAGATGGC TTTAATAAAC 
TCAGTTGAGC TGGACATGCA CGGTCTCTAT ATTGACCAGG CGGAGGCACT CTTGAGTCAG 
AGAAGAGAAG CATTACAAGA GGAGTCCTCT CGGATTCAGA AGTTAACTTA TAACACTGAT 
TCACACAGCA ATGGTGACAC AGCCGGAGAC TGCAGCCCTA CTCAGCAGAG CTTGTCTGAA 
GATGACATTG GATGTGTGCC GTGTGACTGG GAACCAACGG TCGAGCAGAA GGAGGAGATT 
GAAAGTAAAA GAGCTGCTCT GCTGAAAAAG GCACAGGAAG TGTTTGCTGA TGTTCAAAAT 
GAGTTCTGGG ATGTAAAGAA AATCCTCTCC AGATTTGATG AGTGGCGCGT CTCCTTTAAA 
GACTCTTACA ATAATGCCTA CATTGGGCTG TGTCTCCCAA AACTCCTGGC ACCCTTGATC 
CGACACCAGC TCATTGGCTG GAACCCTCTA CAGGCAGAAA GTGAGGACTT TGAGGCATTG 
CCATGGTATT GTGCTGTTGA GAGGTTCTGT CATGGTCAGG GCTACGAGGA ATCAGAGAAC 
ATGGACAAGA CCACACTGCC CACCATAATT GAGAAGACCA TCCTCTCTAA AGTTCAAGGT 
TTTGTAGAGC TGGTCTGGGA CCCTCTTTCT GCCCAGCAGA CTCGAACCCT GACAACACTA 
TGCAGAAGAA TACAGCATGA CTATTCTGTG TTTAATGGCG AACAAAGCAA ACCTGTCAAG 
GCCTTTGTGG AAGCAGTCAT TCAGAGATTA AGGACTGCCG TAGACGATGA TGTCTTTGTC 
CCTCTGTACC CCAAAAAGTT TTTAGAGGAT AAGCGGTCAC CGCAGTTCCA GTTTCAGAAC 
AAACAGTTTT GGTCGGCGGT GAAGTTGCTA GGTAATATGG CATTATGGGG TGGTTTGATT 
CCTGAGCACA TTCTTAAAGA GCTGATGTTG GAGAAACTTC TGGGCCGTTA TTTGATGATA 
ACCATTCTTA ATGAATCTGA TCCAAAACAC ACAGTCCAGA AATGCAAAAA GATTGCAGGT 
TGTTTTCCAG AATCATGGTT TATTGATTTA AATACTGGAT CATCACTACC TCAGTTGCAA 
AACTTCAGCA AACATCTTCT TCAAACCGCA CATCTGATAT TCAAGGACAA CAAGGACTCC 
AGAGGACTTT TATCTGATGT GCTGTTTGTT CTCAAGATCA TCAAGGCTCA TGACAGTATT 
AGGACTATCA CTGAAAAATA TAATTGCAAA GACCTGTTAA AGGCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gcfc2 ( XM_005158732.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158789.1 Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22690
Clone ID Related Accession (Same CDS sequence)	XM_005158732.3 , XM_005158732.4
Accession Version	XM_005158732.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	GC-rich sequence DNA-binding factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158732.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
GAATGGCATG CTCCGAAAGC AGCCCGACAG AAGAGAGTGG AAATCCCAGA AACGCTTCCA 
GTAGTTAGTA TTGGTGTCAT TAAGAAGAGA ATCACTGGAA AATTGGATTC GCTGAGGGAG 
GTACACAGGG CACGGGAGGC GCAGCTAAGG AGGATGCAGC TGGATGTGGA AATGGCCAAA 
ACCTCTTTAG AGGGCCTTGA AAACAACAAT GCAGATGAGC AGCTCCGATT TTACCGCACC 
ATGAACGTTT TCTCACAGAA CCTGCTGGAA TGCCTATCTG AGAAGATGGC TTTAATAAAC 
TCAGTTGAGC TGGACATGCA CGGTCTCTAT ATTGACCAGG CGGAGGCACT CTTGAGTCAG 
AGAAGAGAAG CATTACAAGA GGAGTCCTCT CGGATTCAGA AGTTAACTTA TAACACTGAT 
TCACACAGCA ATGGTGACAC AGCCGGAGAC TGCAGCCCTA CTCAGCAGAG CTTGTCTGAA 
GATGACATTG GATGTGTGCC GTGTGACTGG GAACCAACGG TCGAGCAGAA GGAGGAGATT 
GAAAGTAAAA GAGCTGCTCT GCTGAAAAAG GCACAGGAAG TGTTTGCTGA TGTTCAAAAT 
GAGTTCTGGG ATGTAAAGAA AATCCTCTCC AGATTTGATG AGTGGCGCGT CTCCTTTAAA 
GACTCTTACA ATAATGCCTA CATTGGGCTG TGTCTCCCAA AACTCCTGGC ACCCTTGATC 
CGACACCAGC TCATTGGCTG GAACCCTCTA CAGGCAGAAA GTGAGGACTT TGAGGCATTG 
CCATGGTATT GTGCTGTTGA GAGGTTCTGT CATGGTCAGG GCTACGAGGA ATCAGAGAAC 
ATGGACAAGA CCACACTGCC CACCATAATT GAGAAGACCA TCCTCTCTAA AGTTCAAGGT 
TTTGTAGAGC TGGTCTGGGA CCCTCTTTCT GCCCAGCAGA CTCGAACCCT GACAACACTA 
TGCAGAAGAA TACAGCATGA CTATTCTGTG TTTAATGGCG AACAAAGCAA ACCTGTCAAG 
GCCTTTGTGG AAGCAGTCAT TCAGAGATTA AGGACTGCCG TAGACGATGA TGTCTTTGTC 
CCTCTGTACC CCAAAAAGTT TTTAGAGGAT AAGCGGTCAC CGCAGTTCCA GTTTCAGAAC 
AAACAGTTTT GGTCGGCGGT GAAGTTGCTA GGTAATATGG CATTATGGGG TGGTTTGATT 
CCTGAGCACA TTCTTAAAGA GCTGATGTTG GAGAAACTTC TGGGCCGTTA TTTGATGATA 
ACCATTCTTA ATGAATCTGA TCCAAAACAC ACAGTCCAGA AATGCAAAAA GATTGCAGGT 
TGTTTTCCAG AATCATGGTT TATTGATTTA AATACTGGAT CATCACTACC TCAGTTGCAA 
AACTTCAGCA AACATCTTCT TCAAACCGCA CATCTGATAT TCAAGGACAA CAAGGACTCC 
AGAGGACTTT TATCTGATGT GCTGTTTGTT CTCAAGATCA TCAAGGCTCA TGACAGTATT 
AGGACTATCA CTGAAAAATA TAATTGCAAA GACCTGTTAA AGGCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158789.1
CDS	72..2462
Translation	MFNKKPRRNFRQRKNESSDEDDNGKLAAGEGGKDADLKADLPSKAGISSSSREARECDSSEAEEEASVSALQTKDRKLHTDSKTLSFSDDKDSTEGGFQVKRSVNREVLFQARKKEDSPAPATSSQGKKSKDVSLSDSDSDASAEDIPPDSDEETCSTMSSSSASSLPQRVIIPDARKIHAAKEKRRQARAQQDYISLDSPRTPGSLKEDELSDKEQDSDNDLDDHERRIEFAPKPKTLRERMAEKMGSDSEESFSDSQEEEEQQMWEEQQIGKGVKRHQEWHAPKAARQKRVEIPETLPVVSIGVIKKRITGKLDSLREVHRAREAQLRRMQLDVEMAKTSLEGLENNNADEQLRFYRTMNVFSQNLLECLSEKMALINSVELDMHGLYIDQAEALLSQRREALQEESSRIQKLTYNTDSHSNGDTAGDCSPTQQSLSEDDIGCVPCDWEPTVEQKEEIESKRAALLKKAQEVFADVQNEFWDVKKILSRFDEWRVSFKDSYNNAYIGLCLPKLLAPLIRHQLIGWNPLQAESEDFEALPWYCAVERFCHGQGYEESENMDKTTLPTIIEKTILSKVQGFVELVWDPLSAQQTRTLTTLCRRIQHDYSVFNGEQSKPVKAFVEAVIQRLRTAVDDDVFVPLYPKKFLEDKRSPQFQFQNKQFWSAVKLLGNMALWGGLIPEHILKELMLEKLLGRYLMITILNESDPKHTVQKCKKIAGCFPESWFIDLNTGSSLPQLQNFSKHLLQTAHLIFKDNKDSRGLLSDVLFVLKIIKAHDSIRTITEKYNCKDLLKAL

Target ORF information:

RefSeq Version	XM_005158732.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GC-rich sequence DNA-binding factor 2 (gcfc2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158732.4

ORF Insert Sequence:

ATGTTTAATA AGAAGCCGCG CCGAAATTTC CGGCAAAGGA AAAATGAATC CAGTGATGAA 
GACGACAATG GAAAATTAGC TGCTGGTGAA GGTGGAAAAG ATGCGGATTT AAAGGCAGAT 
TTACCCTCAA AAGCAGGGAT TTCAAGCAGT TCACGAGAAG CTCGAGAGTG TGACTCGAGT 
GAGGCAGAAG AGGAGGCTTC AGTTTCAGCA CTCCAGACCA AAGACAGAAA ACTGCACACA 
GACAGCAAAA CACTCAGCTT CTCTGATGAT AAAGACTCTA CAGAGGGAGG TTTTCAAGTG 
AAACGTTCAG TCAACAGAGA AGTTTTGTTC CAAGCGCGGA AAAAAGAGGA TTCTCCTGCA 
CCTGCAACCA GTTCTCAAGG AAAGAAGAGT AAGGATGTCA GTCTCTCTGA CAGTGATTCT 
GATGCATCAG CTGAAGACAT TCCTCCTGAC AGTGATGAAG AAACCTGTTC TACCATGTCA 
AGTTCCTCCG CTTCTTCACT ACCACAACGA GTCATCATTC CTGATGCCAG AAAGATTCAT 
GCTGCCAAAG AGAAAAGGAG ACAAGCCAGA GCCCAGCAAG ATTACATCTC TCTGGATTCA 
CCTCGAACTC CTGGAAGCTT GAAGGAGGAC GAGCTGAGCG ATAAGGAACA AGACAGTGAC 
AATGATCTGG ATGATCATGA GCGCAGAATT GAGTTTGCAC CCAAACCCAA AACTCTGAGA 
GAGAGAATGG CTGAGAAGAT GGGGAGTGAT AGTGAGGAGA GCTTTTCTGA CAGCCAGGAG 
GAGGAAGAGC AGCAGATGTG GGAGGAACAA CAGATTGGAA AAGGAGTCAA AAGGCACCAA 
GAATGGCATG CTCCGAAAGC AGCCCGACAG AAGAGAGTGG AAATCCCAGA AACGCTTCCA 
GTAGTTAGTA TTGGTGTCAT TAAGAAGAGA ATCACTGGAA AATTGGATTC GCTGAGGGAG 
GTACACAGGG CACGGGAGGC GCAGCTAAGG AGGATGCAGC TGGATGTGGA AATGGCCAAA 
ACCTCTTTAG AGGGCCTTGA AAACAACAAT GCAGATGAGC AGCTCCGATT TTACCGCACC 
ATGAACGTTT TCTCACAGAA CCTGCTGGAA TGCCTATCTG AGAAGATGGC TTTAATAAAC 
TCAGTTGAGC TGGACATGCA CGGTCTCTAT ATTGACCAGG CGGAGGCACT CTTGAGTCAG 
AGAAGAGAAG CATTACAAGA GGAGTCCTCT CGGATTCAGA AGTTAACTTA TAACACTGAT 
TCACACAGCA ATGGTGACAC AGCCGGAGAC TGCAGCCCTA CTCAGCAGAG CTTGTCTGAA 
GATGACATTG GATGTGTGCC GTGTGACTGG GAACCAACGG TCGAGCAGAA GGAGGAGATT 
GAAAGTAAAA GAGCTGCTCT GCTGAAAAAG GCACAGGAAG TGTTTGCTGA TGTTCAAAAT 
GAGTTCTGGG ATGTAAAGAA AATCCTCTCC AGATTTGATG AGTGGCGCGT CTCCTTTAAA 
GACTCTTACA ATAATGCCTA CATTGGGCTG TGTCTCCCAA AACTCCTGGC ACCCTTGATC 
CGACACCAGC TCATTGGCTG GAACCCTCTA CAGGCAGAAA GTGAGGACTT TGAGGCATTG 
CCATGGTATT GTGCTGTTGA GAGGTTCTGT CATGGTCAGG GCTACGAGGA ATCAGAGAAC 
ATGGACAAGA CCACACTGCC CACCATAATT GAGAAGACCA TCCTCTCTAA AGTTCAAGGT 
TTTGTAGAGC TGGTCTGGGA CCCTCTTTCT GCCCAGCAGA CTCGAACCCT GACAACACTA 
TGCAGAAGAA TACAGCATGA CTATTCTGTG TTTAATGGCG AACAAAGCAA ACCTGTCAAG 
GCCTTTGTGG AAGCAGTCAT TCAGAGATTA AGGACTGCCG TAGACGATGA TGTCTTTGTC 
CCTCTGTACC CCAAAAAGTT TTTAGAGGAT AAGCGGTCAC CGCAGTTCCA GTTTCAGAAC 
AAACAGTTTT GGTCGGCGGT GAAGTTGCTA GGTAATATGG CATTATGGGG TGGTTTGATT 
CCTGAGCACA TTCTTAAAGA GCTGATGTTG GAGAAACTTC TGGGCCGTTA TTTGATGATA 
ACCATTCTTA ATGAATCTGA TCCAAAACAC ACAGTCCAGA AATGCAAAAA GATTGCAGGT 
TGTTTTCCAG AATCATGGTT TATTGATTTA AATACTGGAT CATCACTACC TCAGTTGCAA 
AACTTCAGCA AACATCTTCT TCAAACCGCA CATCTGATAT TCAAGGACAA CAAGGACTCC 
AGAGGACTTT TATCTGATGT GCTGTTTGTT CTCAAGATCA TCAAGGCTCA TGACAGTATT 
AGGACTATCA CTGAAAAATA TAATTGCAAA GACCTGTTAA AGGCTCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pervasive yet nonuniform contributions of Dcp2 and Cnot7 to maternal mRNA clearance in zebrafish.
Genes to cells : devoted to molecular & cellular mechanisms22(7)670-678(2017 Jul)
Mishima Y,Tomari Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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gcfc2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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