VAV3B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAV3B
Entrez Gene ID	559145
Full Name	vav 3 guanine nucleotide exchange factor b
Synonyms	fk86g11,si:ch211-255p13.2,wu:fk86g11
General protein information	Preferred Names vav 3 guanine nucleotide exchange factor b Names guanine nucleotide exchange factor VAV3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126393.1	NP_001119865.1	guanine nucleotide exchange factor VAV3
XM_005163323.4	XP_005163380.1	guanine nucleotide exchange factor VAV3 isoform X1
XM_021480557.1	XP_021336232.1	guanine nucleotide exchange factor VAV3 isoform X2
XM_005163323.3	XP_005163380.1	guanine nucleotide exchange factor VAV3 isoform X1

Pathways from BioSystems

Homologies

	VAV3	XP_002801727.1
Mus musculus (house mouse)	Vav3	NP_065251.2
Canis lupus familiaris (dog)	VAV3	XP_537047.3
Bos taurus (cattle)	VAV3	XP_002686208.1
Gallus gallus (chicken)	VAV3	NP_996745.1
Homo sapiens (human)	VAV3	NP_006104.4
Rattus norvegicus (Norway rat)	Vav3	NP_001178643.1
Xenopus tropicalis (tropical clawed frog)	vav3	XP_002932225.2
Danio rerio (zebrafish)	vav3b	NP_001119865.1
Caenorhabditis elegans (roundworm)	vav-1	NP_001041222.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following VAV3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAV3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30850	NM_001126393.1 Latest version!	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa06840	XM_005163323.4 Latest version!	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa42982	XM_021480557.1 Latest version!	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa06840	XM_005163323.3	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAV3B ( NM_001126393.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001119865.1 Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), mRNA.

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CloneID	ODa30850
Clone ID Related Accession (Same CDS sequence)	NM_001126393.1
Accession Version	NM_001126393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor VAV3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR788310.10, CT027695.16 and BX510321.9. On Jul 31, 2008 this sequence version replaced XM_682461.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTAAA 
CAGGCCGAGG TGGACATCAG AAGCTGCTGT CTTTTAGAGA TCAAACAGAC AGAAGCCAAA 
TACACAGAGA CTCTAGAGAC CATTGAGAAG TATTTTATGA TTCCACTGAT GAAATTCATC 
CCTGCAACAG ATATTGATAA GGTTTTCATC AATATACCGG ACCTGGTTAA AGTTCACAAA 
GGTCTAATGG GTGAGATCCA AGACTGTGTT CAGAATAAAA ATGCAGAGAA TCTTCATCAG 
ATCTTCATCA CTTACAAAGA CAGGCTTCTG CTTTATGGAA AATACTGCAG TCATGTAGAA 
TCAGCCATTT CTCTCTTAGA CTTCATCTGT AAGGAAAAAG AGGGCGTTCG ACTGAAACTG 
GAGGAATGTT CAAAAAGAGC CAACAATGGG AAATTCACTT TGAGAGACTT GCTTGTGGTG 
CCAATGCAGC GAGTGCTGAA ATACCCACTT CTGCTTCAGG AACTGGTCAA ATATACAAAT 
GACGGCCCCG AAAAGAGCAA CCTACGAATA GCCCTTGATG CTATGAGAGA CTTAGCACAA 
TATGTAAATG AGGTGAAAAG AGATGTTGAG ACCCTGAGGG AGATTGATCA GTATCAGAGA 
TCCATTGAGA ATTTGAATCA GTCTTTACTT TCTTATGGAA GACCGAAGGG TGACGGTGAA 
GTGCGTTTGG TGTCAAATTT GGACAAGCGC AGACAGGACA GACATATTTT TTTGTTCGAC 
GCTGCTGTAA TCATCTGCAA GAGGCGAGGG GACAACTATG AGATGAAGGA AGTGATCGAC 
CTCAGCCATT TCAAGATCAC CAATAATCTC GCTGATAAGG ACTGCAGAAA GTGGTCCAGC 
GGGTTCTACC TCACCCACCA ACAGGGACAG AGTGGATTTG AGTTCCTATT CAAGACGAAA 
GAGCTGAAGA AGAAATGGCT GGACCAGTTT GAGATGGCTA TATCTAATAT TCGACCAGAA 
AACGCCACGT TTAACTCTCA CCACTTCAGA ATGCACACTT TCGAGAGGGC CACCACCTGC 
AGCTCCTGTC GTATGCTACT CAGGGGGATT TTTAACCAAG GCTACCTTTG TCCTAAATGT 
GGAAACGGGG CTCATAAGGG CTGTCTGGGT AAACTGGGAG TCTGTGGAAG GACAGGTGCT 
GCAGTTCAGA AACCACAGAG AAAGACAACA CCTAGAAAAC AAGAAGACCC AGGTTTGCCA 
AAAATGCTGG TCATCAGAGA TTATTTTGGG GTTTCCAAAC CTCAAGGCGG ACAAGCGTTG 
AATGCTCAGA TTGGTGATGT CATAGAGGTC GTCTACGCTG ATCCTCACAG CTCCTGGTGG 
CAGGGCAAAA TCCTGACGAC GCAAGAGACC GGTTTCTTTC CAAGTGATGC CGTCAAGCCC 
TGCCCATGTG TGCCCAGAAC AGTCGACTAT TCCTCCCAAC CTTGGTTCGC AGGCCCCATG 
GAGAGGCATC ACGCAGAGTC TGAGCTCATG GAGAGAGAGA ACAGCACATA CCTGGTGCGA 
TACCGGAGCC GTGAGAGCAG AGAGTACGCC ATCAGCATCA AGTACAACAA TGATGTGAAG 
CACCTTAAGG TCCTCACTAA GGAGGGCTGT TTCTACATTG CCGAAAGCAG GACGTTCAAG 
AATGTGTTGG GGCTTGTTGA ATATTACAAA CAGCACTCTC TGAAAGAGGG TTTCCGTACT 
CTGGACACTA CTCTGCAGGT TCCCTTTAAG GAACTTGGTA CAGGACTCCG TACTGCTGTT 
TTGACCCCAA GAGTGCTTGG TATTGCGCTG GCGCGATATG ACTTCAGCTC GAGGGACACC 
CGTGAACTCT CACTGCAGGT GGGCGACCTG GTGAAGATCT ATATCAAGTG TACCAATGGA 
TGGTGGAAGG GTGAAGTCAA TGGCCGGGTT GGCTGGTTTC CTTCAACATA TGTGGAAGAG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119865.1
CDS	1..2469
Translation	MEYWRQCSLWLINCKVLPRNHRVTADSAQVFDLAQTLRDGVLLCQLLNNLRPDTINLKEINLRPQMSQFLCLKNIRTFLSACCEEFGMKKSDLFEAFDLFDVRDFGKVIDTLSKLSYTPVAQQSGIRSFPTEESTENEDIYIHLADLIDEELEEDVYDPVYGDIEGGEVYEDLMRTEAAKQAEVDIRSCCLLEIKQTEAKYTETLETIEKYFMIPLMKFIPATDIDKVFINIPDLVKVHKGLMGEIQDCVQNKNAENLHQIFITYKDRLLLYGKYCSHVESAISLLDFICKEKEGVRLKLEECSKRANNGKFTLRDLLVVPMQRVLKYPLLLQELVKYTNDGPEKSNLRIALDAMRDLAQYVNEVKRDVETLREIDQYQRSIENLNQSLLSYGRPKGDGEVRLVSNLDKRRQDRHIFLFDAAVIICKRRGDNYEMKEVIDLSHFKITNNLADKDCRKWSSGFYLTHQQGQSGFEFLFKTKELKKKWLDQFEMAISNIRPENATFNSHHFRMHTFERATTCSSCRMLLRGIFNQGYLCPKCGNGAHKGCLGKLGVCGRTGAAVQKPQRKTTPRKQEDPGLPKMLVIRDYFGVSKPQGGQALNAQIGDVIEVVYADPHSSWWQGKILTTQETGFFPSDAVKPCPCVPRTVDYSSQPWFAGPMERHHAESELMERENSTYLVRYRSRESREYAISIKYNNDVKHLKVLTKEGCFYIAESRTFKNVLGLVEYYKQHSLKEGFRTLDTTLQVPFKELGTGLRTAVLTPRVLGIALARYDFSSRDTRELSLQVGDLVKIYIKCTNGWWKGEVNGRVGWFPSTYVEEEE

Target ORF information:

RefSeq Version	NM_001126393.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126393.1

ORF Insert Sequence:

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTAAA 
CAGGCCGAGG TGGACATCAG AAGCTGCTGT CTTTTAGAGA TCAAACAGAC AGAAGCCAAA 
TACACAGAGA CTCTAGAGAC CATTGAGAAG TATTTTATGA TTCCACTGAT GAAATTCATC 
CCTGCAACAG ATATTGATAA GGTTTTCATC AATATACCGG ACCTGGTTAA AGTTCACAAA 
GGTCTAATGG GTGAGATCCA AGACTGTGTT CAGAATAAAA ATGCAGAGAA TCTTCATCAG 
ATCTTCATCA CTTACAAAGA CAGGCTTCTG CTTTATGGAA AATACTGCAG TCATGTAGAA 
TCAGCCATTT CTCTCTTAGA CTTCATCTGT AAGGAAAAAG AGGGCGTTCG ACTGAAACTG 
GAGGAATGTT CAAAAAGAGC CAACAATGGG AAATTCACTT TGAGAGACTT GCTTGTGGTG 
CCAATGCAGC GAGTGCTGAA ATACCCACTT CTGCTTCAGG AACTGGTCAA ATATACAAAT 
GACGGCCCCG AAAAGAGCAA CCTACGAATA GCCCTTGATG CTATGAGAGA CTTAGCACAA 
TATGTAAATG AGGTGAAAAG AGATGTTGAG ACCCTGAGGG AGATTGATCA GTATCAGAGA 
TCCATTGAGA ATTTGAATCA GTCTTTACTT TCTTATGGAA GACCGAAGGG TGACGGTGAA 
GTGCGTTTGG TGTCAAATTT GGACAAGCGC AGACAGGACA GACATATTTT TTTGTTCGAC 
GCTGCTGTAA TCATCTGCAA GAGGCGAGGG GACAACTATG AGATGAAGGA AGTGATCGAC 
CTCAGCCATT TCAAGATCAC CAATAATCTC GCTGATAAGG ACTGCAGAAA GTGGTCCAGC 
GGGTTCTACC TCACCCACCA ACAGGGACAG AGTGGATTTG AGTTCCTATT CAAGACGAAA 
GAGCTGAAGA AGAAATGGCT GGACCAGTTT GAGATGGCTA TATCTAATAT TCGACCAGAA 
AACGCCACGT TTAACTCTCA CCACTTCAGA ATGCACACTT TCGAGAGGGC CACCACCTGC 
AGCTCCTGTC GTATGCTACT CAGGGGGATT TTTAACCAAG GCTACCTTTG TCCTAAATGT 
GGAAACGGGG CTCATAAGGG CTGTCTGGGT AAACTGGGAG TCTGTGGAAG GACAGGTGCT 
GCAGTTCAGA AACCACAGAG AAAGACAACA CCTAGAAAAC AAGAAGACCC AGGTTTGCCA 
AAAATGCTGG TCATCAGAGA TTATTTTGGG GTTTCCAAAC CTCAAGGCGG ACAAGCGTTG 
AATGCTCAGA TTGGTGATGT CATAGAGGTC GTCTACGCTG ATCCTCACAG CTCCTGGTGG 
CAGGGCAAAA TCCTGACGAC GCAAGAGACC GGTTTCTTTC CAAGTGATGC CGTCAAGCCC 
TGCCCATGTG TGCCCAGAAC AGTCGACTAT TCCTCCCAAC CTTGGTTCGC AGGCCCCATG 
GAGAGGCATC ACGCAGAGTC TGAGCTCATG GAGAGAGAGA ACAGCACATA CCTGGTGCGA 
TACCGGAGCC GTGAGAGCAG AGAGTACGCC ATCAGCATCA AGTACAACAA TGATGTGAAG 
CACCTTAAGG TCCTCACTAA GGAGGGCTGT TTCTACATTG CCGAAAGCAG GACGTTCAAG 
AATGTGTTGG GGCTTGTTGA ATATTACAAA CAGCACTCTC TGAAAGAGGG TTTCCGTACT 
CTGGACACTA CTCTGCAGGT TCCCTTTAAG GAACTTGGTA CAGGACTCCG TACTGCTGTT 
TTGACCCCAA GAGTGCTTGG TATTGCGCTG GCGCGATATG ACTTCAGCTC GAGGGACACC 
CGTGAACTCT CACTGCAGGT GGGCGACCTG GTGAAGATCT ATATCAAGTG TACCAATGGA 
TGGTGGAAGG GTGAAGTCAA TGGCCGGGTT GGCTGGTTTC CTTCAACATA TGTGGAAGAG 
GAGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAV3B ( XM_005163323.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163380.1 Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06840
Clone ID Related Accession (Same CDS sequence)	XM_005163323.4 , XM_005163323.3
Accession Version	XM_005163323.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor VAV3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005163323.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG CTCCAGTGCT GTGTGGGCTT TCCGTTTTGA GTCCCACCAG CTACAATTTT 
GTTCCTCCCT CCCCCTCACC CTTCTGGTCA GTTTTGACCC CAAGAGTGCT TGGTATTGCG 
CTGGCGCGAT ATGACTTCAG CTCGAGGGAC ACCCGTGAAC TCTCACTGCA GGTGGGCGAC 
CTGGTGAAGA TCTATATCAA GTGTACCAAT GGATGGTGGA AGGGTGAAGT CAATGGCCGG 
GTTGGCTGGT TTCCTTCAAC ATATGTGGAA GAGGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163380.1
CDS	283..2844
Translation	MEYWRQCSLWLINCKVLPRNHRVTADSAQVFDLAQTLRDGVLLCQLLNNLRPDTINLKEINLRPQMSQFLCLKNIRTFLSACCEEFGMKKSDLFEAFDLFDVRDFGKVIDTLSKLSYTPVAQQSGIRSFPTEESTENEDIYIHLADLIDEELEEDVYDPVYGDIEGGEVYEDLMRTEAAVSQAKQAEVDIRSCCLLEIKQTEAKYTETLETIEKYFMIPLMKFIPATDIDKVFINIPDLVKVHKGLMGEIQDCVQNKNAENLHQIFITYKDRLLLYGKYCSHVESAISLLDFICKEKEGVRLKLEECSKRANNGKFTLRDLLVVPMQRVLKYPLLLQELVKYTNDGPEKSNLRIALDAMRDLAQYVNEVKRDVETLREIDQYQRSIENLNQSLLSYGRPKGDGEVRLVSNLDKRRQDRHIFLFDAAVIICKRRGDNYEMKEVIDLSHFKITNNLADKDCRKWSSGFYLTHQQGQSGFEFLFKTKELKKKWLDQFEMAISNIRPENATFNSHHFRMHTFERATTCSSCRMLLRGIFNQGYLCPKCGNGAHKGCLGKLGVCGRTGAAVQKPQRKTTPRKQEDPGLPKMLVIRDYFGVSKPQGGQALNAQIGDVIEVVYADPHSSWWQGKILTTQETGFFPSDAVKPCPCVPRTVDYSSQPWFAGPMERHHAESELMERENSTYLVRYRSRESREYAISIKYNNDVKHLKVLTKEGCFYIAESRTFKNVLGLVEYYKQHSLKEGFRTLDTTLQVPFKELGTGLRTAAPVLCGLSVLSPTSYNFVPPSPSPFWSVLTPRVLGIALARYDFSSRDTRELSLQVGDLVKIYIKCTNGWWKGEVNGRVGWFPSTYVEEEE

Target ORF information:

RefSeq Version	XM_005163323.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163323.4

ORF Insert Sequence:

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG CTCCAGTGCT GTGTGGGCTT TCCGTTTTGA GTCCCACCAG CTACAATTTT 
GTTCCTCCCT CCCCCTCACC CTTCTGGTCA GTTTTGACCC CAAGAGTGCT TGGTATTGCG 
CTGGCGCGAT ATGACTTCAG CTCGAGGGAC ACCCGTGAAC TCTCACTGCA GGTGGGCGAC 
CTGGTGAAGA TCTATATCAA GTGTACCAAT GGATGGTGGA AGGGTGAAGT CAATGGCCGG 
GTTGGCTGGT TTCCTTCAAC ATATGTGGAA GAGGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAV3B ( XM_005163323.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163380.1 Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.

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CloneID	ODa06840
Clone ID Related Accession (Same CDS sequence)	XM_005163323.4 , XM_005163323.3
Accession Version	XM_005163323.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	guanine nucleotide exchange factor VAV3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005163323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG CTCCAGTGCT GTGTGGGCTT TCCGTTTTGA GTCCCACCAG CTACAATTTT 
GTTCCTCCCT CCCCCTCACC CTTCTGGTCA GTTTTGACCC CAAGAGTGCT TGGTATTGCG 
CTGGCGCGAT ATGACTTCAG CTCGAGGGAC ACCCGTGAAC TCTCACTGCA GGTGGGCGAC 
CTGGTGAAGA TCTATATCAA GTGTACCAAT GGATGGTGGA AGGGTGAAGT CAATGGCCGG 
GTTGGCTGGT TTCCTTCAAC ATATGTGGAA GAGGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163380.1
CDS	283..2844
Translation	MEYWRQCSLWLINCKVLPRNHRVTADSAQVFDLAQTLRDGVLLCQLLNNLRPDTINLKEINLRPQMSQFLCLKNIRTFLSACCEEFGMKKSDLFEAFDLFDVRDFGKVIDTLSKLSYTPVAQQSGIRSFPTEESTENEDIYIHLADLIDEELEEDVYDPVYGDIEGGEVYEDLMRTEAAVSQAKQAEVDIRSCCLLEIKQTEAKYTETLETIEKYFMIPLMKFIPATDIDKVFINIPDLVKVHKGLMGEIQDCVQNKNAENLHQIFITYKDRLLLYGKYCSHVESAISLLDFICKEKEGVRLKLEECSKRANNGKFTLRDLLVVPMQRVLKYPLLLQELVKYTNDGPEKSNLRIALDAMRDLAQYVNEVKRDVETLREIDQYQRSIENLNQSLLSYGRPKGDGEVRLVSNLDKRRQDRHIFLFDAAVIICKRRGDNYEMKEVIDLSHFKITNNLADKDCRKWSSGFYLTHQQGQSGFEFLFKTKELKKKWLDQFEMAISNIRPENATFNSHHFRMHTFERATTCSSCRMLLRGIFNQGYLCPKCGNGAHKGCLGKLGVCGRTGAAVQKPQRKTTPRKQEDPGLPKMLVIRDYFGVSKPQGGQALNAQIGDVIEVVYADPHSSWWQGKILTTQETGFFPSDAVKPCPCVPRTVDYSSQPWFAGPMERHHAESELMERENSTYLVRYRSRESREYAISIKYNNDVKHLKVLTKEGCFYIAESRTFKNVLGLVEYYKQHSLKEGFRTLDTTLQVPFKELGTGLRTAAPVLCGLSVLSPTSYNFVPPSPSPFWSVLTPRVLGIALARYDFSSRDTRELSLQVGDLVKIYIKCTNGWWKGEVNGRVGWFPSTYVEEEE

Target ORF information:

RefSeq Version	XM_005163323.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005163323.3

ORF Insert Sequence:

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG CTCCAGTGCT GTGTGGGCTT TCCGTTTTGA GTCCCACCAG CTACAATTTT 
GTTCCTCCCT CCCCCTCACC CTTCTGGTCA GTTTTGACCC CAAGAGTGCT TGGTATTGCG 
CTGGCGCGAT ATGACTTCAG CTCGAGGGAC ACCCGTGAAC TCTCACTGCA GGTGGGCGAC 
CTGGTGAAGA TCTATATCAA GTGTACCAAT GGATGGTGGA AGGGTGAAGT CAATGGCCGG 
GTTGGCTGGT TTCCTTCAAC ATATGTGGAA GAGGAGGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAV3B ( XM_021480557.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336232.1 Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42982
Clone ID Related Accession (Same CDS sequence)	XM_021480557.1
Accession Version	XM_021480557.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	guanine nucleotide exchange factor VAV3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG TTTTGACCCC AAGAGTGCTT GGTATTGCGC TGGCGCGATA TGACTTCAGC 
TCGAGGGACA CCCGTGAACT CTCACTGCAG GTGGGCGACC TGGTGAAGAT CTATATCAAG 
TGTACCAATG GATGGTGGAA GGGTGAAGTC AATGGCCGGG TTGGCTGGTT TCCTTCAACA 
TATGTGGAAG AGGAGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336232.1
CDS	283..2763
Translation	MEYWRQCSLWLINCKVLPRNHRVTADSAQVFDLAQTLRDGVLLCQLLNNLRPDTINLKEINLRPQMSQFLCLKNIRTFLSACCEEFGMKKSDLFEAFDLFDVRDFGKVIDTLSKLSYTPVAQQSGIRSFPTEESTENEDIYIHLADLIDEELEEDVYDPVYGDIEGGEVYEDLMRTEAAVSQAKQAEVDIRSCCLLEIKQTEAKYTETLETIEKYFMIPLMKFIPATDIDKVFINIPDLVKVHKGLMGEIQDCVQNKNAENLHQIFITYKDRLLLYGKYCSHVESAISLLDFICKEKEGVRLKLEECSKRANNGKFTLRDLLVVPMQRVLKYPLLLQELVKYTNDGPEKSNLRIALDAMRDLAQYVNEVKRDVETLREIDQYQRSIENLNQSLLSYGRPKGDGEVRLVSNLDKRRQDRHIFLFDAAVIICKRRGDNYEMKEVIDLSHFKITNNLADKDCRKWSSGFYLTHQQGQSGFEFLFKTKELKKKWLDQFEMAISNIRPENATFNSHHFRMHTFERATTCSSCRMLLRGIFNQGYLCPKCGNGAHKGCLGKLGVCGRTGAAVQKPQRKTTPRKQEDPGLPKMLVIRDYFGVSKPQGGQALNAQIGDVIEVVYADPHSSWWQGKILTTQETGFFPSDAVKPCPCVPRTVDYSSQPWFAGPMERHHAESELMERENSTYLVRYRSRESREYAISIKYNNDVKHLKVLTKEGCFYIAESRTFKNVLGLVEYYKQHSLKEGFRTLDTTLQVPFKELGTGLRTAVLTPRVLGIALARYDFSSRDTRELSLQVGDLVKIYIKCTNGWWKGEVNGRVGWFPSTYVEEEE

Target ORF information:

RefSeq Version	XM_021480557.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vav 3 guanine nucleotide exchange factor b (vav3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480557.1

ORF Insert Sequence:

ATGGAGTACT GGAGGCAGTG CAGTCTGTGG CTGATCAATT GTAAAGTTTT GCCCCGGAAT 
CATCGGGTCA CGGCGGACTC GGCGCAGGTT TTTGATTTGG CCCAAACTCT TCGGGATGGG 
GTGCTTCTGT GCCAGCTGCT GAACAATCTC AGACCAGACA CCATTAACCT GAAGGAGATC 
AATCTCAGAC CACAGATGTC ACAGTTTCTC TGTCTGAAGA ATATCCGTAC ATTCCTGTCT 
GCATGCTGTG AAGAATTTGG GATGAAGAAG AGTGACTTGT TTGAGGCCTT CGATCTCTTT 
GATGTCCGTG ATTTTGGAAA GGTCATCGAC ACACTATCAA AACTCTCATA TACGCCTGTT 
GCTCAACAGA GTGGCATCAG GTCGTTTCCC ACAGAGGAAA GCACTGAAAA TGAAGACATA 
TACATCCATT TGGCAGATCT CATCGACGAG GAGCTGGAAG AAGATGTTTA TGACCCTGTG 
TATGGAGACA TTGAAGGAGG AGAGGTGTAT GAGGACCTGA TGAGGACTGA AGCTGCTGTG 
TCTCAAGCGA AACAGGCCGA GGTGGACATC AGAAGCTGCT GTCTTTTAGA GATCAAACAG 
ACAGAAGCCA AATACACAGA GACTCTAGAG ACCATTGAGA AGTATTTTAT GATTCCACTG 
ATGAAATTCA TCCCTGCAAC AGATATTGAT AAGGTTTTCA TCAATATACC GGACCTGGTT 
AAAGTTCACA AAGGTCTAAT GGGTGAGATC CAAGACTGTG TTCAGAATAA AAATGCAGAG 
AATCTTCATC AGATCTTCAT CACTTACAAA GACAGGCTTC TGCTTTATGG AAAATACTGC 
AGTCATGTAG AATCAGCCAT TTCTCTCTTA GACTTCATCT GTAAGGAAAA AGAGGGCGTT 
CGACTGAAAC TGGAGGAATG TTCAAAAAGA GCCAACAATG GGAAATTCAC TTTGAGAGAC 
TTGCTTGTGG TGCCAATGCA GCGAGTGCTG AAATACCCAC TTCTGCTTCA GGAACTGGTC 
AAATATACAA ATGACGGCCC CGAAAAGAGC AACCTACGAA TAGCCCTTGA TGCTATGAGA 
GACTTAGCAC AATATGTAAA TGAGGTGAAA AGAGATGTTG AGACCCTGAG GGAGATTGAT 
CAGTATCAGA GATCCATTGA GAATTTGAAT CAGTCTTTAC TTTCTTATGG AAGACCGAAG 
GGTGACGGTG AAGTGCGTTT GGTGTCAAAT TTGGACAAGC GCAGACAGGA CAGACATATT 
TTTTTGTTCG ACGCTGCTGT AATCATCTGC AAGAGGCGAG GGGACAACTA TGAGATGAAG 
GAAGTGATCG ACCTCAGCCA TTTCAAGATC ACCAATAATC TCGCTGATAA GGACTGCAGA 
AAGTGGTCCA GCGGGTTCTA CCTCACCCAC CAACAGGGAC AGAGTGGATT TGAGTTCCTA 
TTCAAGACGA AAGAGCTGAA GAAGAAATGG CTGGACCAGT TTGAGATGGC TATATCTAAT 
ATTCGACCAG AAAACGCCAC GTTTAACTCT CACCACTTCA GAATGCACAC TTTCGAGAGG 
GCCACCACCT GCAGCTCCTG TCGTATGCTA CTCAGGGGGA TTTTTAACCA AGGCTACCTT 
TGTCCTAAAT GTGGAAACGG GGCTCATAAG GGCTGTCTGG GTAAACTGGG AGTCTGTGGA 
AGGACAGGTG CTGCAGTTCA GAAACCACAG AGAAAGACAA CACCTAGAAA ACAAGAAGAC 
CCAGGTTTGC CAAAAATGCT GGTCATCAGA GATTATTTTG GGGTTTCCAA ACCTCAAGGC 
GGACAAGCGT TGAATGCTCA GATTGGTGAT GTCATAGAGG TCGTCTACGC TGATCCTCAC 
AGCTCCTGGT GGCAGGGCAA AATCCTGACG ACGCAAGAGA CCGGTTTCTT TCCAAGTGAT 
GCCGTCAAGC CCTGCCCATG TGTGCCCAGA ACAGTCGACT ATTCCTCCCA ACCTTGGTTC 
GCAGGCCCCA TGGAGAGGCA TCACGCAGAG TCTGAGCTCA TGGAGAGAGA GAACAGCACA 
TACCTGGTGC GATACCGGAG CCGTGAGAGC AGAGAGTACG CCATCAGCAT CAAGTACAAC 
AATGATGTGA AGCACCTTAA GGTCCTCACT AAGGAGGGCT GTTTCTACAT TGCCGAAAGC 
AGGACGTTCA AGAATGTGTT GGGGCTTGTT GAATATTACA AACAGCACTC TCTGAAAGAG 
GGTTTCCGTA CTCTGGACAC TACTCTGCAG GTTCCCTTTA AGGAACTTGG TACAGGACTC 
CGTACTGCTG TTTTGACCCC AAGAGTGCTT GGTATTGCGC TGGCGCGATA TGACTTCAGC 
TCGAGGGACA CCCGTGAACT CTCACTGCAG GTGGGCGACC TGGTGAAGAT CTATATCAAG 
TGTACCAATG GATGGTGGAA GGGTGAAGTC AATGGCCGGG TTGGCTGGTT TCCTTCAACA 
TATGTGGAAG AGGAGGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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VAV3B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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