UBR2 cDNA ORF clone, Danio rerio(Zebrafish)

The following UBR2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145429 XM_021472602.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa145430 XM_021472603.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
ODa54844 XM_017358928.2
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa54840 XM_017358924.2
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54841 XM_017358925.2
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54842 XM_017358926.2
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54843 XM_017358927.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa18374 XM_005156497.4
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa18375 XM_005156498.4
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa18376 XM_001921555.7
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa18375 XM_005156498.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa18376 XM_001921555.6 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa54840 XM_017358924.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54842 XM_017358926.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54841 XM_017358925.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa54844 XM_017358928.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa18374 XM_005156497.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145429
    Clone ID Related Accession (Same CDS sequence) XM_021472602.1
    Accession Version XM_021472602.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2841bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
    CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC
    AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA
    GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG
    AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG
    GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG
    ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC
    AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC
    ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG
    ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT
    GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG
    GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG
    ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG
    GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT
    GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG
    GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC
    CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC
    AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG
    GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG
    GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC
    GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA
    CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC
    TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA
    GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT
    CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA
    GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC
    ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGATGAA
    GAGAAGCCTT TGTTTGGAAG TTTACCATGC AGACAGGACG ACTGTTTGAG TTCTCTGACG
    CGGTTTGGCT CTGCCTGCTG GACAGTCTCC TCTCATACCA CTGTACAGAA TCACTTCATC
    AGGCTCTTAG CAGCCCTTGT TCCTGATCCC CGGGTGGAGA ATACCCCTTG TATTCTGGAT
    GTAGACATGT TTCACTTGCT GGTGAGTTTG GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG
    GATTCCTCAG GGCTCAGCGC AGATGCTGCA CAACTGCACT TCTTGCACCT GATCACGGTC
    GCTCACGTTG TCCAGATCCT CCTGACCTCT TTACCAGAGG AGGTGCGGAT GGAGCAGGAG
    ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG AGTGTGTGTT TGCTCTACAA TACTCTCAGG
    AGTCTGCTGG GCCGTGGCTT GCGGGAGGTG AGCTCAGGCT GGCATCTGTG GCTCTGTGTG
    AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC GCCGCTCTCT TCTTCCACTA TCTCAACGGA
    GCTCCAGCAC CACCGGAGTT ACACGCTCTA GGTGCGGGTG AATGGGAGGC TCTGTGTGGA
    TACCTGTGTC TGCCTTCCAA CCTGCTACAG CTCTACTTCA ACAATCTAGA CATCATGGAG
    CCTCTGTTGC ATGGGTGGTG TTCTCATCCA GCTCTTCAGG CCCTTCAGAG CAGCGTGACT
    CTCATCAGGT TTCCCAGAGA GTCCAATCAT CTCATCGATC TGCCGGGCGA CTACAGCGCG
    TTGATCAACC AGGCCTCCAG CTTCACGTGT CCTAAATCTG GAGGAGATAA GTCTCGCGTT
    CCTACTCTGT GTCTGGTGTG CGGTGCCATG CTCTGCTCTC AGAGCTACTG CTGTCAGACG
    GAGCTGGAAG GAGAAGACGT GGGGGCCTGT ACTGCACACA CTTTCACCTG CGGAGCCGGA
    GTCGGCATCT TCCTCAGGGT GAGGGAAAGT CAGGTCCTGT TCCTGGCTGG CAAAACTAAG
    GGCTGTTTTT ATGCTCCCCC ATACCTGGAT GACTATGGAG AAACAGACCA AGGGCTAAGA
    CGGGGGAACC CATTACACTT GTGCCATGAG CGCTACAGAA AGATCCAGAA GCTGTGGCGT
    CAGCACAGCA TCACGGAGGA GATCGGCCAC GCTCAGGAGG CCAATCAGAC TTTGGTGGGC
    ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328277.1
    CDS23..2863
    Translation

    Target ORF information:

    RefSeq Version XM_021472602.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
    CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC
    AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA
    GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG
    AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG
    GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG
    ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC
    AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC
    ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG
    ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT
    GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG
    GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG
    ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG
    GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT
    GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG
    GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC
    CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC
    AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG
    GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG
    GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC
    GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA
    CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC
    TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA
    GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT
    CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA
    GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC
    ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGATGAA
    GAGAAGCCTT TGTTTGGAAG TTTACCATGC AGACAGGACG ACTGTTTGAG TTCTCTGACG
    CGGTTTGGCT CTGCCTGCTG GACAGTCTCC TCTCATACCA CTGTACAGAA TCACTTCATC
    AGGCTCTTAG CAGCCCTTGT TCCTGATCCC CGGGTGGAGA ATACCCCTTG TATTCTGGAT
    GTAGACATGT TTCACTTGCT GGTGAGTTTG GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG
    GATTCCTCAG GGCTCAGCGC AGATGCTGCA CAACTGCACT TCTTGCACCT GATCACGGTC
    GCTCACGTTG TCCAGATCCT CCTGACCTCT TTACCAGAGG AGGTGCGGAT GGAGCAGGAG
    ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG AGTGTGTGTT TGCTCTACAA TACTCTCAGG
    AGTCTGCTGG GCCGTGGCTT GCGGGAGGTG AGCTCAGGCT GGCATCTGTG GCTCTGTGTG
    AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC GCCGCTCTCT TCTTCCACTA TCTCAACGGA
    GCTCCAGCAC CACCGGAGTT ACACGCTCTA GGTGCGGGTG AATGGGAGGC TCTGTGTGGA
    TACCTGTGTC TGCCTTCCAA CCTGCTACAG CTCTACTTCA ACAATCTAGA CATCATGGAG
    CCTCTGTTGC ATGGGTGGTG TTCTCATCCA GCTCTTCAGG CCCTTCAGAG CAGCGTGACT
    CTCATCAGGT TTCCCAGAGA GTCCAATCAT CTCATCGATC TGCCGGGCGA CTACAGCGCG
    TTGATCAACC AGGCCTCCAG CTTCACGTGT CCTAAATCTG GAGGAGATAA GTCTCGCGTT
    CCTACTCTGT GTCTGGTGTG CGGTGCCATG CTCTGCTCTC AGAGCTACTG CTGTCAGACG
    GAGCTGGAAG GAGAAGACGT GGGGGCCTGT ACTGCACACA CTTTCACCTG CGGAGCCGGA
    GTCGGCATCT TCCTCAGGGT GAGGGAAAGT CAGGTCCTGT TCCTGGCTGG CAAAACTAAG
    GGCTGTTTTT ATGCTCCCCC ATACCTGGAT GACTATGGAG AAACAGACCA AGGGCTAAGA
    CGGGGGAACC CATTACACTT GTGCCATGAG CGCTACAGAA AGATCCAGAA GCTGTGGCGT
    CAGCACAGCA TCACGGAGGA GATCGGCCAC GCTCAGGAGG CCAATCAGAC TTTGGTGGGC
    ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145430
    Clone ID Related Accession (Same CDS sequence) XM_021472603.1
    Accession Version XM_021472603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
    CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC
    AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA
    GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG
    AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG
    GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG
    ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC
    AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC
    ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG
    ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT
    GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG
    GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG
    ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG
    GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT
    GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG
    GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC
    CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC
    AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG
    GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG
    GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC
    GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA
    CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC
    TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA
    GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT
    CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA
    GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC
    ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGTTCCT
    TGTTTTCTGC CACTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328278.1
    CDS23..1663
    Translation

    Target ORF information:

    RefSeq Version XM_021472603.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
    CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC
    AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA
    GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG
    AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG
    GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG
    ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC
    AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC
    ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG
    ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT
    GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG
    GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG
    ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG
    GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT
    GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG
    GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC
    CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC
    AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG
    GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG
    GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC
    GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA
    CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC
    TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA
    GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT
    CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA
    GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC
    ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGTTCCT
    TGTTTTCTGC CACTTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54844
    Clone ID Related Accession (Same CDS sequence) XM_017358928.1 , XM_017358928.2
    Accession Version XM_017358928.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214417.1
    CDS78..4193
    Translation

    Target ORF information:

    RefSeq Version XM_017358928.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54844
    Clone ID Related Accession (Same CDS sequence) XM_017358928.1 , XM_017358928.2
    Accession Version XM_017358928.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214417.1
    CDS90..4205
    Translation

    Target ORF information:

    RefSeq Version XM_017358928.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358928.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54840
    Clone ID Related Accession (Same CDS sequence) XM_017358924.1 , XM_017358924.2
    Accession Version XM_017358924.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214413.1
    CDS67..5382
    Translation

    Target ORF information:

    RefSeq Version XM_017358924.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358924.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54840
    Clone ID Related Accession (Same CDS sequence) XM_017358924.1 , XM_017358924.2
    Accession Version XM_017358924.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214413.1
    CDS71..5386
    Translation

    Target ORF information:

    RefSeq Version XM_017358924.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358924.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54841
    Clone ID Related Accession (Same CDS sequence) XM_017358925.2 , XM_017358925.1
    Accession Version XM_017358925.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG
    GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG
    CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214414.1
    CDS72..5387
    Translation

    Target ORF information:

    RefSeq Version XM_017358925.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358925.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG
    GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG
    CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54841
    Clone ID Related Accession (Same CDS sequence) XM_017358925.2 , XM_017358925.1
    Accession Version XM_017358925.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG
    GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG
    CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214414.1
    CDS68..5383
    Translation

    Target ORF information:

    RefSeq Version XM_017358925.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG
    GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG
    CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT
    CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC
    CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG
    GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT
    CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT
    CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG
    GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA
    GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC
    TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC
    ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG
    GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA
    GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC
    CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT
    CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54842
    Clone ID Related Accession (Same CDS sequence) XM_017358926.1 , XM_017358926.2
    Accession Version XM_017358926.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG
    GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA
    GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG
    AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG
    GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT
    TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC
    GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA
    CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG
    ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT
    GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG
    CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA
    CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG
    TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG
    CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG
    GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG
    AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA
    CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG
    ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG
    TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG
    GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC
    TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA
    AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC
    ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG
    CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC
    AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG
    ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC
    CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG
    CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT
    GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG
    CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG
    CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC
    TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT
    AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG
    CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG
    TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG
    GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA
    ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC
    CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC
    TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC
    CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC
    AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG
    AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC
    GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT
    GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT
    GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT
    CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG
    TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC
    AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC
    TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT
    ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC
    CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA
    CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG
    AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG
    AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT
    TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA
    GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC
    CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG
    ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC
    AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214415.1
    CDS67..5379
    Translation

    Target ORF information:

    RefSeq Version XM_017358926.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG
    GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA
    GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG
    AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG
    GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT
    TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC
    GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA
    CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG
    ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT
    GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG
    CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA
    CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG
    TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG
    CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG
    GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG
    AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA
    CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG
    ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG
    TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG
    GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC
    TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA
    AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC
    ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG
    CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC
    AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG
    ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC
    CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG
    CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT
    GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG
    CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG
    CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC
    TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT
    AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG
    CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG
    TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG
    GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA
    ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC
    CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC
    TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC
    CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC
    AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG
    AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC
    GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT
    GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT
    GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT
    CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG
    TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC
    AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC
    TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT
    ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC
    CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA
    CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG
    AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG
    AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT
    TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA
    GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC
    CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG
    ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC
    AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54842
    Clone ID Related Accession (Same CDS sequence) XM_017358926.1 , XM_017358926.2
    Accession Version XM_017358926.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG
    GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA
    GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG
    AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG
    GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT
    TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC
    GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA
    CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG
    ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT
    GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG
    CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA
    CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG
    TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG
    CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG
    GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG
    AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA
    CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG
    ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG
    TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG
    GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC
    TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA
    AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC
    ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG
    CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC
    AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG
    ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC
    CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG
    CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT
    GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG
    CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG
    CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC
    TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT
    AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG
    CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG
    TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG
    GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA
    ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC
    CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC
    TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC
    CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC
    AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG
    AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC
    GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT
    GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT
    GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT
    CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG
    TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC
    AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC
    TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT
    ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC
    CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA
    CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG
    AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG
    AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT
    TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA
    GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC
    CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG
    ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC
    AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214415.1
    CDS70..5382
    Translation

    Target ORF information:

    RefSeq Version XM_017358926.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358926.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG
    GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA
    GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG
    AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG
    GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT
    TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC
    GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA
    CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG
    ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT
    GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG
    CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA
    CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG
    TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG
    CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG
    GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG
    AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA
    CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG
    ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG
    TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG
    GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC
    TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA
    AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC
    ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG
    CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC
    AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG
    ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC
    CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG
    CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT
    GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG
    CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG
    CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC
    TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT
    AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG
    CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG
    TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG
    GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA
    ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC
    CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC
    TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC
    CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC
    AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG
    AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC
    GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT
    GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT
    GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT
    CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG
    TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC
    AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC
    TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT
    ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC
    CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA
    CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG
    AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG
    AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT
    TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA
    GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC
    CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG
    ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC
    AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54843
    Clone ID Related Accession (Same CDS sequence) XM_017358927.1
    Accession Version XM_017358927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214416.1
    CDS68..5380
    Translation

    Target ORF information:

    RefSeq Version XM_017358927.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA
    CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT
    TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC
    AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG
    GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA
    GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC
    AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG
    AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG
    CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG
    TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC
    GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC
    TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT
    GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG
    GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC
    ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA
    GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG
    CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT
    CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG
    CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT
    CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT
    GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG
    AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG
    ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC
    AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG
    CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC
    TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC
    CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG
    AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC
    GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC
    TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG
    AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG
    TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT
    CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG
    CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA
    GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT
    GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG
    GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT
    GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG
    CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG
    AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG
    TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC
    AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC
    GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC
    GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC
    AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG
    TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT
    TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT
    TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC
    ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG
    TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA
    ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT
    GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG
    ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC
    CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA
    TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT
    TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA
    CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC
    CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA
    GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG
    CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG
    CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC
    TACAATACTC TCAGGAGTCT GCTGGGCCGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG
    TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC
    TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG
    GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA
    GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG
    AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC
    GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT
    AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC
    TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC
    TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT
    GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC
    CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG
    AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG
    ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18374
    Clone ID Related Accession (Same CDS sequence) XM_005156497.4 , XM_005156497.3
    Accession Version XM_005156497.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG
    GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT
    GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA
    CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC
    TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT
    GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG
    ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC
    CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC
    GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG
    AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC
    CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT
    ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC
    GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC
    GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC
    ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA
    GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT
    CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC
    TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA
    TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT
    CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT
    GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA
    AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT
    ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC
    AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA
    GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT
    TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC
    AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA
    GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG
    CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG
    GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG
    TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT
    CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC
    AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG
    AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC
    TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC
    AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG
    GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC
    AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC
    ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC
    TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG
    CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT
    GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC
    ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG
    CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG
    CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA
    TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA
    CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT
    GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT
    GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC
    ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG
    CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC
    ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA
    TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC
    TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT
    CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT
    TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT
    GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC
    TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG
    GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG
    GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA
    GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT
    CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA
    CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT
    CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT
    CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG
    TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC
    ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC
    TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA
    AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG
    GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT
    GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC
    CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156554.1
    CDS70..5343
    Translation

    Target ORF information:

    RefSeq Version XM_005156497.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156497.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG
    GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT
    GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA
    CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC
    TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT
    GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG
    ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC
    CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC
    GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG
    AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC
    CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT
    ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC
    GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC
    GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC
    ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA
    GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT
    CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC
    TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA
    TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT
    CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT
    GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA
    AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT
    ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC
    AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA
    GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT
    TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC
    AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA
    GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG
    CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG
    GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG
    TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT
    CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC
    AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG
    AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC
    TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC
    AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG
    GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC
    AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC
    ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC
    TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG
    CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT
    GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC
    ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG
    CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG
    CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA
    TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA
    CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT
    GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT
    GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC
    ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG
    CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC
    ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA
    TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC
    TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT
    CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT
    TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT
    GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC
    TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG
    GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG
    GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA
    GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT
    CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA
    CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT
    CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT
    CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG
    TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC
    ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC
    TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA
    AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG
    GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT
    GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC
    CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18374
    Clone ID Related Accession (Same CDS sequence) XM_005156497.4 , XM_005156497.3
    Accession Version XM_005156497.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG
    GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT
    GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA
    CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC
    TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT
    GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG
    ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC
    CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC
    GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG
    AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC
    CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT
    ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC
    GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC
    GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC
    ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA
    GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT
    CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC
    TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA
    TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT
    CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT
    GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA
    AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT
    ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC
    AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA
    GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT
    TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC
    AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA
    GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG
    CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG
    GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG
    TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT
    CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC
    AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG
    AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC
    TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC
    AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG
    GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC
    AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC
    ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC
    TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG
    CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT
    GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC
    ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG
    CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG
    CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA
    TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA
    CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT
    GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT
    GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC
    ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG
    CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC
    ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA
    TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC
    TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT
    CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT
    TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT
    GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC
    TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG
    GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG
    GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA
    GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT
    CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA
    CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT
    CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT
    CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG
    TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC
    ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC
    TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA
    AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG
    GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT
    GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC
    CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156554.1
    CDS67..5340
    Translation

    Target ORF information:

    RefSeq Version XM_005156497.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG
    GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT
    GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA
    CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC
    TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT
    GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG
    ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC
    CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC
    GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG
    AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC
    CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT
    ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC
    GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC
    GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC
    ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA
    GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT
    CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC
    TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA
    TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT
    CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT
    GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA
    AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT
    ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC
    AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA
    GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT
    TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC
    AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA
    GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG
    CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG
    GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG
    TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT
    CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC
    AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG
    AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC
    TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC
    AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG
    GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC
    AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC
    ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC
    TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG
    CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT
    GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC
    ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG
    CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG
    CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA
    TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA
    CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT
    GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT
    GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC
    ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG
    CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC
    ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA
    TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC
    TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT
    CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT
    TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT
    GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC
    TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG
    GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG
    GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA
    GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT
    CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA
    CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT
    CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT
    CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG
    TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC
    ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC
    TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA
    AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG
    GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT
    GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC
    CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18375
    Clone ID Related Accession (Same CDS sequence) XM_005156498.4 , XM_005156498.3
    Accession Version XM_005156498.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG
    CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG
    CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156555.1
    CDS69..5339
    Translation

    Target ORF information:

    RefSeq Version XM_005156498.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156498.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG
    CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG
    CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18375
    Clone ID Related Accession (Same CDS sequence) XM_005156498.4 , XM_005156498.3
    Accession Version XM_005156498.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG
    CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG
    CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156555.1
    CDS67..5337
    Translation

    Target ORF information:

    RefSeq Version XM_005156498.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156498.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG
    CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG
    CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18376
    Clone ID Related Accession (Same CDS sequence) XM_001921555.7 , XM_001921555.6
    Accession Version XM_001921555.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921555.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT
    CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG
    CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001921590.3
    CDS70..5340
    Translation

    Target ORF information:

    RefSeq Version XM_001921555.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001921555.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT
    CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG
    CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18376
    Clone ID Related Accession (Same CDS sequence) XM_001921555.7 , XM_001921555.6
    Accession Version XM_001921555.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921555.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT
    CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG
    CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001921590.3
    CDS67..5337
    Translation

    Target ORF information:

    RefSeq Version XM_001921555.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001921555.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
    TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG
    TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT
    CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT
    GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT
    TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT
    CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC
    AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG
    AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT
    TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC
    AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG
    CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC
    GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT
    TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT
    TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG
    GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC
    TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT
    CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC
    TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC
    ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT
    CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG
    CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC
    ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG
    TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA
    GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG
    TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG
    GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC
    TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG
    AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT
    TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC
    CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA
    GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT
    CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT
    GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT
    TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG
    GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG
    CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT
    AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG
    ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA
    CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA
    CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG
    TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG
    ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT
    CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT
    CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC
    ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC
    ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC
    ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA
    CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC
    GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC
    ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT
    CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG
    CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC
    GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG
    CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG
    CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG
    GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC
    ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG
    CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC
    TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC
    CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT
    CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG
    CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT
    CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC
    TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC
    ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC
    CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC
    CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC
    AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC
    TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC
    CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG
    GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA
    CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT
    TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG
    AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG
    AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC
    GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA
    GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG
    CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA
    GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT
    CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT
    CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG
    CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT
    ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT
    CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT
    GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG
    CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC
    GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.