UBR2 gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_017358925.2	XP_017214414.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_005156498.4	XP_005156555.1	E3 ubiquitin-protein ligase UBR2 isoform X5
XM_017358926.1	XP_017214415.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_005156498.3	XP_005156555.1	E3 ubiquitin-protein ligase UBR2 isoform X6
XM_021472602.1	XP_021328277.1	E3 ubiquitin-protein ligase UBR2 isoform X8
XM_021472603.1	XP_021328278.1	E3 ubiquitin-protein ligase UBR2 isoform X9
XM_001921555.7	XP_001921590.3	E3 ubiquitin-protein ligase UBR2 isoform X6
XM_017358924.1	XP_017214413.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_017358927.1	XP_017214416.1	E3 ubiquitin-protein ligase UBR2 isoform X4
XM_001921555.6	XP_001921590.3	E3 ubiquitin-protein ligase UBR2 isoform X7
XM_017358928.1	XP_017214417.1	E3 ubiquitin-protein ligase UBR2 isoform X8
XM_017358924.2	XP_017214413.1	E3 ubiquitin-protein ligase UBR2 isoform X1
XM_017358926.2	XP_017214415.1	E3 ubiquitin-protein ligase UBR2 isoform X3
XM_005156497.4	XP_005156554.1	E3 ubiquitin-protein ligase UBR2 isoform X4
XM_017358925.1	XP_017214414.1	E3 ubiquitin-protein ligase UBR2 isoform X2
XM_017358928.2	XP_017214417.1	E3 ubiquitin-protein ligase UBR2 isoform X7
XM_005156497.3	XP_005156554.1	E3 ubiquitin-protein ligase UBR2 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145429	XM_021472602.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa145430	XM_021472603.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54844	XM_017358928.2 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa54840	XM_017358924.2 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54841	XM_017358925.2 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54842	XM_017358926.2 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54843	XM_017358927.1 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa18374	XM_005156497.4 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18375	XM_005156498.4 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18376	XM_001921555.7 Latest version!	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa18375	XM_005156498.3	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18376	XM_001921555.6	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa54840	XM_017358924.1	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54842	XM_017358926.1	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54841	XM_017358925.1	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa54844	XM_017358928.1	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa18374	XM_005156497.3	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

UBR2 ( XM_021472602.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328277.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa145429
Clone ID Related Accession (Same CDS sequence)	XM_021472602.1
Accession Version	XM_021472602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC 
AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA 
GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG 
AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG 
GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG 
ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC 
AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC 
ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG 
ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT 
GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG 
GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG 
ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG 
GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT 
GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG 
GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC 
CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC 
AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG 
GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG 
GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC 
GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA 
CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC 
TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA 
GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT 
CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA 
GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC 
ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGATGAA 
GAGAAGCCTT TGTTTGGAAG TTTACCATGC AGACAGGACG ACTGTTTGAG TTCTCTGACG 
CGGTTTGGCT CTGCCTGCTG GACAGTCTCC TCTCATACCA CTGTACAGAA TCACTTCATC 
AGGCTCTTAG CAGCCCTTGT TCCTGATCCC CGGGTGGAGA ATACCCCTTG TATTCTGGAT 
GTAGACATGT TTCACTTGCT GGTGAGTTTG GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG 
GATTCCTCAG GGCTCAGCGC AGATGCTGCA CAACTGCACT TCTTGCACCT GATCACGGTC 
GCTCACGTTG TCCAGATCCT CCTGACCTCT TTACCAGAGG AGGTGCGGAT GGAGCAGGAG 
ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG AGTGTGTGTT TGCTCTACAA TACTCTCAGG 
AGTCTGCTGG GCCGTGGCTT GCGGGAGGTG AGCTCAGGCT GGCATCTGTG GCTCTGTGTG 
AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC GCCGCTCTCT TCTTCCACTA TCTCAACGGA 
GCTCCAGCAC CACCGGAGTT ACACGCTCTA GGTGCGGGTG AATGGGAGGC TCTGTGTGGA 
TACCTGTGTC TGCCTTCCAA CCTGCTACAG CTCTACTTCA ACAATCTAGA CATCATGGAG 
CCTCTGTTGC ATGGGTGGTG TTCTCATCCA GCTCTTCAGG CCCTTCAGAG CAGCGTGACT 
CTCATCAGGT TTCCCAGAGA GTCCAATCAT CTCATCGATC TGCCGGGCGA CTACAGCGCG 
TTGATCAACC AGGCCTCCAG CTTCACGTGT CCTAAATCTG GAGGAGATAA GTCTCGCGTT 
CCTACTCTGT GTCTGGTGTG CGGTGCCATG CTCTGCTCTC AGAGCTACTG CTGTCAGACG 
GAGCTGGAAG GAGAAGACGT GGGGGCCTGT ACTGCACACA CTTTCACCTG CGGAGCCGGA 
GTCGGCATCT TCCTCAGGGT GAGGGAAAGT CAGGTCCTGT TCCTGGCTGG CAAAACTAAG 
GGCTGTTTTT ATGCTCCCCC ATACCTGGAT GACTATGGAG AAACAGACCA AGGGCTAAGA 
CGGGGGAACC CATTACACTT GTGCCATGAG CGCTACAGAA AGATCCAGAA GCTGTGGCGT 
CAGCACAGCA TCACGGAGGA GATCGGCCAC GCTCAGGAGG CCAATCAGAC TTTGGTGGGC 
ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328277.1
CDS	23..2863
Translation	MERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWLCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_021472602.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472602.1

ORF Insert Sequence:

ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC 
AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA 
GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG 
AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG 
GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG 
ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC 
AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC 
ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG 
ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT 
GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG 
GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG 
ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG 
GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT 
GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG 
GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC 
CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC 
AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG 
GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG 
GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC 
GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA 
CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC 
TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA 
GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT 
CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA 
GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC 
ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGATGAA 
GAGAAGCCTT TGTTTGGAAG TTTACCATGC AGACAGGACG ACTGTTTGAG TTCTCTGACG 
CGGTTTGGCT CTGCCTGCTG GACAGTCTCC TCTCATACCA CTGTACAGAA TCACTTCATC 
AGGCTCTTAG CAGCCCTTGT TCCTGATCCC CGGGTGGAGA ATACCCCTTG TATTCTGGAT 
GTAGACATGT TTCACTTGCT GGTGAGTTTG GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG 
GATTCCTCAG GGCTCAGCGC AGATGCTGCA CAACTGCACT TCTTGCACCT GATCACGGTC 
GCTCACGTTG TCCAGATCCT CCTGACCTCT TTACCAGAGG AGGTGCGGAT GGAGCAGGAG 
ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG AGTGTGTGTT TGCTCTACAA TACTCTCAGG 
AGTCTGCTGG GCCGTGGCTT GCGGGAGGTG AGCTCAGGCT GGCATCTGTG GCTCTGTGTG 
AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC GCCGCTCTCT TCTTCCACTA TCTCAACGGA 
GCTCCAGCAC CACCGGAGTT ACACGCTCTA GGTGCGGGTG AATGGGAGGC TCTGTGTGGA 
TACCTGTGTC TGCCTTCCAA CCTGCTACAG CTCTACTTCA ACAATCTAGA CATCATGGAG 
CCTCTGTTGC ATGGGTGGTG TTCTCATCCA GCTCTTCAGG CCCTTCAGAG CAGCGTGACT 
CTCATCAGGT TTCCCAGAGA GTCCAATCAT CTCATCGATC TGCCGGGCGA CTACAGCGCG 
TTGATCAACC AGGCCTCCAG CTTCACGTGT CCTAAATCTG GAGGAGATAA GTCTCGCGTT 
CCTACTCTGT GTCTGGTGTG CGGTGCCATG CTCTGCTCTC AGAGCTACTG CTGTCAGACG 
GAGCTGGAAG GAGAAGACGT GGGGGCCTGT ACTGCACACA CTTTCACCTG CGGAGCCGGA 
GTCGGCATCT TCCTCAGGGT GAGGGAAAGT CAGGTCCTGT TCCTGGCTGG CAAAACTAAG 
GGCTGTTTTT ATGCTCCCCC ATACCTGGAT GACTATGGAG AAACAGACCA AGGGCTAAGA 
CGGGGGAACC CATTACACTT GTGCCATGAG CGCTACAGAA AGATCCAGAA GCTGTGGCGT 
CAGCACAGCA TCACGGAGGA GATCGGCCAC GCTCAGGAGG CCAATCAGAC TTTGGTGGGC 
ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_021472603.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021328278.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa145430
Clone ID Related Accession (Same CDS sequence)	XM_021472603.1
Accession Version	XM_021472603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394861.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC 
AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA 
GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG 
AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG 
GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG 
ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC 
AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC 
ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG 
ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT 
GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG 
GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG 
ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG 
GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT 
GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG 
GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC 
CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC 
AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG 
GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG 
GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC 
GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA 
CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC 
TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA 
GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT 
CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA 
GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC 
ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGTTCCT 
TGTTTTCTGC CACTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328278.1
CDS	23..1663
Translation	MERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVVPCFLPLK

Target ORF information:

RefSeq Version	XM_021472603.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021472603.1

ORF Insert Sequence:

ATGGAGAGGG TCATCGATAG TGTGGCTTTG TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA 
CTCTATAAAC TCAGACCAGA GTGTGCCAAG ATGTTCAATC TTTACTTCCA CCATTACTCC 
AGAGCTGACC AGTCTAAGGC TGAAGAAGCT CAAAGAAAAA TAAAGAGGCA AAATGGGGAA 
GATTCAGTTC TGCCTCCACC AGTCCTGCCT CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG 
AACATCCTGC AGTGTGACGT GCTGCTGGGC ATTCTAGGAG CTGTTCTGCA GTGGGCAATG 
GAGCCTAGTG GAGGACACTG GTCTGAGTCC ATGCTTCAGA GGGTGCTGCA TCTCATTGGG 
ATGGGGCTGG TGGAAGAACA GCAGCATCTC ATCAGCAGCG CAGAAGACTA TAATGATACC 
AACTTCAATT TCACTCTCAA AATCTCCAGA CCCGGCGAGG CTCCTGCTAA CACTGCCAGC 
ATCTTGGCTT TACTGGAGAC GCTGCAGAAC GCTCCTCATC TGGAGGTGCA CAAGGACATG 
ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC ATCAAAACCA CGCGTGAGTG CACAGCCAGT 
GCGCCCCCCA GTGACAGCTC AGGGCACAGT CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG 
GCGGAGCGGA AGAGGAAAGC GGAGATGGCG CGACTCAGGA GAGAGAAAAT CATGGCTCAG 
ATGTCAGAGA TGCAGAGACA CTTCATCAAC GAGAACAAAG AGTTGTTCCA GCAGAGCCTG 
GAGGACCTGG ACCCCTCCAC ATCCTCCACG CTGGAGCACA GCCCCAGCTC GTGTGACAGT 
GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG CGAGCAGGTG GCAGCGACCG CAGGCAGGTG 
GTGACATGTA TCCTGTGCCA GGAGGAGCAG GAGATCCGTA GCGACGGCAA AGCCATGGTC 
CTCGCTGCAT TCGTCCAGCG CTCCACTGTC ATGTCCAAAA ACCGCAAGAG ACCACCACAC 
AACCCAGATA AATATGACCC CCTGTTCATG CACCCCGACC TGTCGTTCGG CACACACACG 
GGCAGCTGTG GACACATCAT GCACTCGCAC TGCTGGCAGA GGTATTTTGA GGCGGTGCAG 
GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC CGTGTGCACA CTAGTTATGA CGTGGAGAAC 
GGAGAGTTTT TGTGTCCACT CTGCGAATGT CTGAGTAACA CAGTCATTCC TCTGCTGCCA 
CTCACTAAGA CCTGCTGCAG CTCTGAACAG CCTGATCTGA GCCAGTGGTT AAACATCACC 
TCTCATCAGA TGAAAGCCCT GATCTCTGCT CACAGGAAGT CCAAAACCAG CAGTGCTGAA 
GCTGCTGATG AAACAGACGG TGTCAATGAC TGTCCACCAC CTGATGGCTT CAGACTAGAT 
CACACTCCAA AAAATCCATA TTCAAGCACC ATAAAGGAGA TGCTGACTAC GTTTGGGACA 
GCCACGTATA AAGTGGGTCT GAAAGTGCAC CCGAATGAGC AGGACCCCCG AGTGCCAATC 
ATGTGCTGGG GCAGCTGTGC CTACACCATC CAGTCTATAG AGAGATTACT GGTGGTTCCT 
TGTTTTCTGC CACTTAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358928.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214417.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

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CloneID	ODa54844
Clone ID Related Accession (Same CDS sequence)	XM_017358928.1 , XM_017358928.2
Accession Version	XM_017358928.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214417.1
CDS	78..4193
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVVPCFLPLK

Target ORF information:

RefSeq Version	XM_017358928.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358928.1

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358928.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214417.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa54844
Clone ID Related Accession (Same CDS sequence)	XM_017358928.1 , XM_017358928.2
Accession Version	XM_017358928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214417.1
CDS	90..4205
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVVPCFLPLK

Target ORF information:

RefSeq Version	XM_017358928.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358928.2

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG TTCCTTGTTT TCTGCCACTT AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358924.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214413.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

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CloneID	ODa54840
Clone ID Related Accession (Same CDS sequence)	XM_017358924.1 , XM_017358924.2
Accession Version	XM_017358924.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214413.1
CDS	67..5382
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358924.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358924.1

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358924.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214413.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

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CloneID	ODa54840
Clone ID Related Accession (Same CDS sequence)	XM_017358924.1 , XM_017358924.2
Accession Version	XM_017358924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214413.1
CDS	71..5386
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358924.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358924.2

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358925.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214414.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

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CloneID	ODa54841
Clone ID Related Accession (Same CDS sequence)	XM_017358925.2 , XM_017358925.1
Accession Version	XM_017358925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG 
GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG 
CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214414.1
CDS	72..5387
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARSYQRLQTDFMEDDHERVVSVTSLSVQLFTAPTVARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358925.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358925.2

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG 
GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG 
CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358925.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214414.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

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CloneID	ODa54841
Clone ID Related Accession (Same CDS sequence)	XM_017358925.2 , XM_017358925.1
Accession Version	XM_017358925.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG 
GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG 
CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214414.1
CDS	68..5383
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARSYQRLQTDFMEDDHERVVSVTSLSVQLFTAPTVARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358925.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358925.1

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AGTTACCAGC GCCTTCAGAC AGATTTTATG 
GAGGACGATC ACGAGCGTGT AGTGTCAGTG ACCTCTCTGT CAGTGCAGCT CTTCACCGCG 
CCTACTGTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGT GGCTTGCGCG AGGTGAGCTC AGGCTGGCAT 
CTGTGGCGCT GTGTGAAAGC TGGAATCCTG CCTTATCTGA GAGCCGCCGC TCTCTTCTTC 
CACTATCTCA ACGGAGCTCC AGCACCACCG GAGTTACACG CTCTAGGTGC GGGTGAATGG 
GAGGCTCTGT GTGGATACCT GTGTCTGCCT TCCAACCTGC TACAGCTCTA CTTCAACAAT 
CTAGACATCA TGGAGCCTCT GTTGCATGGG TGGTGTTCTC ATCCAGCTCT TCAGGCTCTT 
CAGAGCAGCG TGACTCTCAT CAGGTTTCCC AGAGAGTCCA ATCATCTCAT CGATCTGCCG 
GGCGACTACA GCGCGTTGAT CAACCAGGCC TCCAGCTTCA CGTGTCCTAA ATCTGGAGGA 
GATAAGTCTC GCGTTCCTAC TCTGTGTCTG GTGTGCGGTG CCATGCTCTG CTCTCAGAGC 
TACTGCTGTC AGACGGAGCT GGAAGGAGAA GACGTGGGGG CCTGTACCGC ACACACTTTC 
ACCTGCGGAG CCGGAGTCGG CATCTTCCTC AGGGTGAGGG AAAGTCAGGT CCTGTTCCTG 
GCTGGCAAAA CTAAGGGCTG TTTTTATGCT CCCCCATACC TGGATGACTA TGGAGAAACA 
GACCAAGGGC TAAGACGGGG GAACCCATTA CACTTGTGCC ATGAGCGCTA CAGAAAGATC 
CAGAAGCTGT GGCGTCAGCA CAGCATCACG GAGGAGATCG GCCACGCTCA GGAGGCCAAT 
CAGACTTTGG TGGGCATCGA CTGGCAGCAT CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358926.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214415.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

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CloneID	ODa54842
Clone ID Related Accession (Same CDS sequence)	XM_017358926.1 , XM_017358926.2
Accession Version	XM_017358926.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG 
GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA 
GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG 
AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG 
GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT 
TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC 
GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA 
CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG 
ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT 
GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG 
CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA 
CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG 
TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG 
CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG 
GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG 
AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA 
CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG 
ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG 
TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG 
GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC 
TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA 
AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC 
ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG 
CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC 
AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG 
ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC 
CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG 
CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT 
GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG 
CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG 
CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC 
TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT 
AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG 
CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG 
TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG 
GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA 
ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC 
CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC 
TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC 
CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC 
AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG 
AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC 
GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT 
GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT 
GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT 
CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG 
TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC 
AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC 
TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT 
ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC 
CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA 
CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG 
AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG 
AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT 
TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA 
GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC 
CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG 
ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC 
AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214415.1
CDS	67..5379
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358926.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358926.1

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG 
GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA 
GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG 
AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG 
GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT 
TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC 
GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA 
CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG 
ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT 
GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG 
CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA 
CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG 
TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG 
CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG 
GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG 
AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA 
CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG 
ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG 
TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG 
GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC 
TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA 
AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC 
ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG 
CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC 
AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG 
ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC 
CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG 
CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT 
GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG 
CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG 
CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC 
TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT 
AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG 
CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG 
TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG 
GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA 
ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC 
CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC 
TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC 
CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC 
AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG 
AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC 
GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT 
GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT 
GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT 
CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG 
TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC 
AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC 
TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT 
ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC 
CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA 
CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG 
AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG 
AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT 
TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA 
GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC 
CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG 
ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC 
AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358926.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214415.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

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CloneID	ODa54842
Clone ID Related Accession (Same CDS sequence)	XM_017358926.1 , XM_017358926.2
Accession Version	XM_017358926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG 
GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA 
GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG 
AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG 
GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT 
TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC 
GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA 
CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG 
ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT 
GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG 
CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA 
CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG 
TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG 
CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG 
GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG 
AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA 
CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG 
ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG 
TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG 
GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC 
TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA 
AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC 
ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG 
CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC 
AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG 
ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC 
CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG 
CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT 
GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG 
CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG 
CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC 
TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT 
AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG 
CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG 
TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG 
GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA 
ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC 
CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC 
TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC 
CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC 
AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG 
AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC 
GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT 
GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT 
GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT 
CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG 
TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC 
AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC 
TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT 
ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC 
CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA 
CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG 
AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG 
AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT 
TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA 
GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC 
CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG 
ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC 
AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214415.1
CDS	70..5382
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358926.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358926.2

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG ATGCTCTGAG GAGGATCTTG TGTCAGGTGG GTCTGCAGAG AGGACCGGAG 
GGAGAAAACT CCTCTCTAGT GGACAAACTC ATGCTCTGTG ACTCCAAGAT GTGGAAAGGA 
GCGAGAAATG TTTACCATCA GCTCTTTATG AGCAGTCTAC TCATGGATCC GAAGTACAAG 
AAACTTTTTG CTATCCAGTT TGCGAGGAAC TATGAGCGCT TGCAGAGCGA CTATGTGAAG 
GACGACCACG ACAGGGAGTT CTCCGTCACT GATCTTTCTG TGCAAATGTT CACCGTGCCT 
TCTCTGGCGC GGATGCTGAT CACAGATGAG AATCTGATGA CCACCATCAT CCGCACGTTC 
GTGGATCACC TCAGACACCG AGACCTGCAG GGCCGCTTCC AGTTTGAACG CTACACCGCA 
CAACAGGCCT TCAAATTCCG CCGAGTCCAG AGCCTTATCG GAGACCTCAA GTATGTCTTG 
ATCAGCCGTC CAACAGAGTG GACCGACCAA CTCAGGGAGA AGTATCTGGA AGGTTTTGAT 
GCTTTCTTGG AGTTGCTTAA ATGCATGCAG GGAATGGACC CGGTTGTGCG CCAGGTGGGG 
CAGCACATAG AGATGGAGCC AGAATGGGAG GCAGCGTTTA CCTTACAGAT GAAGCTCACA 
CATATCATCT CCATGATGCA GGAGTGGTGT GCCAGTGACG AGAGAGTGCT GATTGAAGCG 
TATAAGAAGA GCCTGAGTGC ACTGACTCAC TGCCACAGCG GTTTCACAGA CGGAGAGCAG 
CCTATAACCC TCAGCATGTG CGGTCACTCC GTCGACACCA TCCGCTACTG CGTCTCTCAG 
GAGAAAGTGA GCATACACCT GCCTGTGTCC CGGCTTCTAG CAGGTTTACA TGCTCTGCTG 
AGCAAAATTG AGGTTGCCTA TCGATTCCCC GAGCAGCTTC CTCTGAGTGA ACTAAGTCCA 
CCCATGCTGA TCGAACACCC GCTCCGCTGC CTTGTACTCT GTGCCCAGGT GCATGCTGGG 
ATGTGGAGAA GAAACGGCTT CTCTTTGGTT AATCAGATAT ATTATTATCA CAATGTGAAG 
TGTAGAGTGG AGATGTTTGA TAAAGACCTC ATCATGCTTC AGGCTGGAGC TTCCATGATG 
GATCCAAACC ACTTCCTGAT GATCATGTTG AGTCGTTTTG AGCTCTATCA TATCTTCAGC 
TCAGCAGACT GCAGAAAGAG ATACAGCCGA GAGAACGCAA ATAAGGATGT GGTTCAGCAA 
AATAACACCC TAATAGAGGA AATGCTGCAC TTGATCATTA TGATCGTGGG TGAGCGCTAC 
ACAGCAGGTG TCGGTCAGGT GGAGATCACG GATGAGATCA GACGAGAGAT CGTTCACCAG 
CTCTGCATTC GACCCATGGC CCACAGCGAG CTGGTCAAAG CACTGCCTGA GAATGAGAAC 
AAGGAGACAG GAATGGAGAG GGTCATCGAT AGTGTGGCTT TGTTTAAGAA GCCTGGCGTG 
ACGGGTAGAG GACTCTATAA ACTCAGACCA GAGTGTGCCA AGATGTTCAA TCTTTACTTC 
CACCATTACT CCAGAGCTGA CCAGTCTAAG GCTGAAGAAG CTCAAAGAAA AATAAAGAGG 
CAAAATGGGG AAGATTCAGT TCTGCCTCCA CCAGTCCTGC CTCGCTTCTG TCCGCTGTTT 
GCCAGTCTGG TGAACATCCT GCAGTGTGAC GTGCTGCTGG GCATTCTAGG AGCTGTTCTG 
CAGTGGGCAA TGGAGCCTAG TGGAGGACAC TGGTCTGAGT CCATGCTTCA GAGGGTGCTG 
CATCTCATTG GGATGGGGCT GGTGGAAGAA CAGCAGCATC TCATCAGCAG CGCAGAAGAC 
TATAATGATA CCAACTTCAA TTTCACTCTC AAAATCTCCA GACCCGGCGA GGCTCCTGCT 
AACACTGCCA GCATCTTGGC TTTACTGGAG ACGCTGCAGA ACGCTCCTCA TCTGGAGGTG 
CACAAGGACA TGATCCGCTG GATCCTCAAG ACTGTGGCCT ACATCAAAAC CACGCGTGAG 
TGCACAGCCA GTGCGCCCCC CAGTGACAGC TCAGGGCACA GTCAAGAGGA GACGGTGCGG 
GATAAGGAAA AGGCGGAGCG GAAGAGGAAA GCGGAGATGG CGCGACTCAG GAGAGAGAAA 
ATCATGGCTC AGATGTCAGA GATGCAGAGA CACTTCATCA ACGAGAACAA AGAGTTGTTC 
CAGCAGAGCC TGGAGGACCT GGACCCCTCC ACATCCTCCA CGCTGGAGCA CAGCCCCAGC 
TCGTGTGACA GTGCTCTGGT GTGTGTGGGC CCGCGGCGCT GGCGAGCAGG TGGCAGCGAC 
CGCAGGCAGG TGGTGACATG TATCCTGTGC CAGGAGGAGC AGGAGATCCG TAGCGACGGC 
AAAGCCATGG TCCTCGCTGC ATTCGTCCAG CGCTCCACTG TCATGTCCAA AAACCGCAAG 
AGACCACCAC ACAACCCAGA TAAATATGAC CCCCTGTTCA TGCACCCCGA CCTGTCGTTC 
GGCACACACA CGGGCAGCTG TGGACACATC ATGCACTCGC ACTGCTGGCA GAGGTATTTT 
GAGGCGGTGC AGGCTAAAGA GCAGCGCAGG CAGCAGAGGC TCCGTGTGCA CACTAGTTAT 
GACGTGGAGA ACGGAGAGTT TTTGTGTCCA CTCTGCGAAT GTCTGAGTAA CACAGTCATT 
CCTCTGCTGC CACTCACTAA GACCTGCTGC AGCTCTGAAC AGCCTGATCT GAGCCAGTGG 
TTAAACATCA CCTCTCATCA GATGAAAGCC CTGATCTCTG CTCACAGGAA GTCCAAAACC 
AGCAGTGCTG AAGCTGCTGA TGAAACAGAC GGTGTCAATG ACTGTCCACC ACCTGATGGC 
TTCAGACTAG ATCACACTCC AAAAAATCCA TATTCAAGCA CCATAAAGGA GATGCTGACT 
ACGTTTGGGA CAGCCACGTA TAAAGTGGGT CTGAAAGTGC ACCCGAATGA GCAGGACCCC 
CGAGTGCCAA TCATGTGCTG GGGCAGCTGT GCCTACACCA TCCAGTCTAT AGAGAGATTA 
CTGGTGGATG AAGAGAAGCC TTTGTTTGGA AGTTTACCAT GCAGACAGGA CGACTGTTTG 
AGTTCTCTGA CGCGGTTTGG CTCTGCCTGC TGGACAGTCT CCTCTCATAC CACTGTACAG 
AATCACTTCA TCAGGCTCTT AGCAGCCCTT GTTCCTGATC CCCGGGTGGA GAATACCCCT 
TGTATTCTGG ATGTAGACAT GTTTCACTTG CTGGTGAGTT TGGTGCTGTC CCATCCAGCA 
GTTCTCTGTC TGGATTCCTC AGGGCTCAGC GCAGATGCTG CACAACTGCA CTTCTTGCAC 
CTGATCACAG TCGCTCACGT TGTCCAGATC CTCCTGACCT CTTTACCTGA GGAGGTGCGG 
ATGGAGCAGG AGACTGGAGG AGCCGAGAGA GAAGAGGAGG AGAGTGTGTG TTTGCTCTAC 
AATACTCTCA GGAGTCTGCT GGGCCGTGGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_017358927.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017214416.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

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CloneID	ODa54843
Clone ID Related Accession (Same CDS sequence)	XM_017358927.1
Accession Version	XM_017358927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214416.1
CDS	68..5380
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVVNTLQSTSTKPLAWRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_017358927.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358927.1

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGGTA AATACTCTGC AATCAACATC TACAAAACCA 
CTTGCCTGGA GAAACACGAG TCGGCAGACG AAACCTCTGC GGGTTCAGGT CATGCACTCT 
TCTGTAGTGG CTCATCAGTG TTTTGCACTC AAAGCTCTGA ACTGGCTTGG CCAAACCATC 
AGATACTCAG CAGATGCTCT GAGGAGGATC TTGTGTCAGG TGGGTCTGCA GAGAGGACCG 
GAGGGAGAAA ACTCCTCTCT AGTGGACAAA CTCATGCTCT GTGACTCCAA GATGTGGAAA 
GGAGCGAGAA ATGTTTACCA TCAGCTCTTT ATGAGCAGTC TACTCATGGA TCCGAAGTAC 
AAGAAACTTT TTGCTATCCA GTTTGCGAGG AACTATGAGC GCTTGCAGAG CGACTATGTG 
AAGGACGACC ACGACAGGGA GTTCTCCGTC ACTGATCTTT CTGTGCAAAT GTTCACCGTG 
CCTTCTCTGG CGCGGATGCT GATCACAGAT GAGAATCTGA TGACCACCAT CATCCGCACG 
TTCGTGGATC ACCTCAGACA CCGAGACCTG CAGGGCCGCT TCCAGTTTGA ACGCTACACC 
GCACAACAGG CCTTCAAATT CCGCCGAGTC CAGAGCCTTA TCGGAGACCT CAAGTATGTC 
TTGATCAGCC GTCCAACAGA GTGGACCGAC CAACTCAGGG AGAAGTATCT GGAAGGTTTT 
GATGCTTTCT TGGAGTTGCT TAAATGCATG CAGGGAATGG ACCCGGTTGT GCGCCAGGTG 
GGGCAGCACA TAGAGATGGA GCCAGAATGG GAGGCAGCGT TTACCTTACA GATGAAGCTC 
ACACATATCA TCTCCATGAT GCAGGAGTGG TGTGCCAGTG ACGAGAGAGT GCTGATTGAA 
GCGTATAAGA AGAGCCTGAG TGCACTGACT CACTGCCACA GCGGTTTCAC AGACGGAGAG 
CAGCCTATAA CCCTCAGCAT GTGCGGTCAC TCCGTCGACA CCATCCGCTA CTGCGTCTCT 
CAGGAGAAAG TGAGCATACA CCTGCCTGTG TCCCGGCTTC TAGCAGGTTT ACATGCTCTG 
CTGAGCAAAA TTGAGGTTGC CTATCGATTC CCCGAGCAGC TTCCTCTGAG TGAACTAAGT 
CCACCCATGC TGATCGAACA CCCGCTCCGC TGCCTTGTAC TCTGTGCCCA GGTGCATGCT 
GGGATGTGGA GAAGAAACGG CTTCTCTTTG GTTAATCAGA TATATTATTA TCACAATGTG 
AAGTGTAGAG TGGAGATGTT TGATAAAGAC CTCATCATGC TTCAGGCTGG AGCTTCCATG 
ATGGATCCAA ACCACTTCCT GATGATCATG TTGAGTCGTT TTGAGCTCTA TCATATCTTC 
AGCTCAGCAG ACTGCAGAAA GAGATACAGC CGAGAGAACG CAAATAAGGA TGTGGTTCAG 
CAAAATAACA CCCTAATAGA GGAAATGCTG CACTTGATCA TTATGATCGT GGGTGAGCGC 
TACACAGCAG GTGTCGGTCA GGTGGAGATC ACGGATGAGA TCAGACGAGA GATCGTTCAC 
CAGCTCTGCA TTCGACCCAT GGCCCACAGC GAGCTGGTCA AAGCACTGCC TGAGAATGAG 
AACAAGGAGA CAGGAATGGA GAGGGTCATC GATAGTGTGG CTTTGTTTAA GAAGCCTGGC 
GTGACGGGTA GAGGACTCTA TAAACTCAGA CCAGAGTGTG CCAAGATGTT CAATCTTTAC 
TTCCACCATT ACTCCAGAGC TGACCAGTCT AAGGCTGAAG AAGCTCAAAG AAAAATAAAG 
AGGCAAAATG GGGAAGATTC AGTTCTGCCT CCACCAGTCC TGCCTCGCTT CTGTCCGCTG 
TTTGCCAGTC TGGTGAACAT CCTGCAGTGT GACGTGCTGC TGGGCATTCT AGGAGCTGTT 
CTGCAGTGGG CAATGGAGCC TAGTGGAGGA CACTGGTCTG AGTCCATGCT TCAGAGGGTG 
CTGCATCTCA TTGGGATGGG GCTGGTGGAA GAACAGCAGC ATCTCATCAG CAGCGCAGAA 
GACTATAATG ATACCAACTT CAATTTCACT CTCAAAATCT CCAGACCCGG CGAGGCTCCT 
GCTAACACTG CCAGCATCTT GGCTTTACTG GAGACGCTGC AGAACGCTCC TCATCTGGAG 
GTGCACAAGG ACATGATCCG CTGGATCCTC AAGACTGTGG CCTACATCAA AACCACGCGT 
GAGTGCACAG CCAGTGCGCC CCCCAGTGAC AGCTCAGGGC ACAGTCAAGA GGAGACGGTG 
CGGGATAAGG AAAAGGCGGA GCGGAAGAGG AAAGCGGAGA TGGCGCGACT CAGGAGAGAG 
AAAATCATGG CTCAGATGTC AGAGATGCAG AGACACTTCA TCAACGAGAA CAAAGAGTTG 
TTCCAGCAGA GCCTGGAGGA CCTGGACCCC TCCACATCCT CCACGCTGGA GCACAGCCCC 
AGCTCGTGTG ACAGTGCTCT GGTGTGTGTG GGCCCGCGGC GCTGGCGAGC AGGTGGCAGC 
GACCGCAGGC AGGTGGTGAC ATGTATCCTG TGCCAGGAGG AGCAGGAGAT CCGTAGCGAC 
GGCAAAGCCA TGGTCCTCGC TGCATTCGTC CAGCGCTCCA CTGTCATGTC CAAAAACCGC 
AAGAGACCAC CACACAACCC AGATAAATAT GACCCCCTGT TCATGCACCC CGACCTGTCG 
TTCGGCACAC ACACGGGCAG CTGTGGACAC ATCATGCACT CGCACTGCTG GCAGAGGTAT 
TTTGAGGCGG TGCAGGCTAA AGAGCAGCGC AGGCAGCAGA GGCTCCGTGT GCACACTAGT 
TATGACGTGG AGAACGGAGA GTTTTTGTGT CCACTCTGCG AATGTCTGAG TAACACAGTC 
ATTCCTCTGC TGCCACTCAC TAAGACCTGC TGCAGCTCTG AACAGCCTGA TCTGAGCCAG 
TGGTTAAACA TCACCTCTCA TCAGATGAAA GCCCTGATCT CTGCTCACAG GAAGTCCAAA 
ACCAGCAGTG CTGAAGCTGC TGATGAAACA GACGGTGTCA ATGACTGTCC ACCACCTGAT 
GGCTTCAGAC TAGATCACAC TCCAAAAAAT CCATATTCAA GCACCATAAA GGAGATGCTG 
ACTACGTTTG GGACAGCCAC GTATAAAGTG GGTCTGAAAG TGCACCCGAA TGAGCAGGAC 
CCCCGAGTGC CAATCATGTG CTGGGGCAGC TGTGCCTACA CCATCCAGTC TATAGAGAGA 
TTACTGGTGG ATGAAGAGAA GCCTTTGTTT GGAAGTTTAC CATGCAGACA GGACGACTGT 
TTGAGTTCTC TGACGCGGTT TGGCTCTGCC TGCTGGACAG TCTCCTCTCA TACCACTGTA 
CAGAATCACT TCATCAGGCT CTTAGCAGCC CTTGTTCCTG ATCCCCGGGT GGAGAATACC 
CCTTGTATTC TGGATGTAGA CATGTTTCAC TTGCTGGTGA GTTTGGTGCT GTCCCATCCA 
GCAGTTCTCT GTCTGGATTC CTCAGGGCTC AGCGCAGATG CTGCACAACT GCACTTCTTG 
CACCTGATCA CAGTCGCTCA CGTTGTCCAG ATCCTCCTGA CCTCTTTACC TGAGGAGGTG 
CGGATGGAGC AGGAGACTGG AGGAGCCGAG AGAGAAGAGG AGGAGAGTGT GTGTTTGCTC 
TACAATACTC TCAGGAGTCT GCTGGGCCGC TTGCGCGAGG TGAGCTCAGG CTGGCATCTG 
TGGCGCTGTG TGAAAGCTGG AATCCTGCCT TATCTGAGAG CCGCCGCTCT CTTCTTCCAC 
TATCTCAACG GAGCTCCAGC ACCACCGGAG TTACACGCTC TAGGTGCGGG TGAATGGGAG 
GCTCTGTGTG GATACCTGTG TCTGCCTTCC AACCTGCTAC AGCTCTACTT CAACAATCTA 
GACATCATGG AGCCTCTGTT GCATGGGTGG TGTTCTCATC CAGCTCTTCA GGCTCTTCAG 
AGCAGCGTGA CTCTCATCAG GTTTCCCAGA GAGTCCAATC ATCTCATCGA TCTGCCGGGC 
GACTACAGCG CGTTGATCAA CCAGGCCTCC AGCTTCACGT GTCCTAAATC TGGAGGAGAT 
AAGTCTCGCG TTCCTACTCT GTGTCTGGTG TGCGGTGCCA TGCTCTGCTC TCAGAGCTAC 
TGCTGTCAGA CGGAGCTGGA AGGAGAAGAC GTGGGGGCCT GTACCGCACA CACTTTCACC 
TGCGGAGCCG GAGTCGGCAT CTTCCTCAGG GTGAGGGAAA GTCAGGTCCT GTTCCTGGCT 
GGCAAAACTA AGGGCTGTTT TTATGCTCCC CCATACCTGG ATGACTATGG AGAAACAGAC 
CAAGGGCTAA GACGGGGGAA CCCATTACAC TTGTGCCATG AGCGCTACAG AAAGATCCAG 
AAGCTGTGGC GTCAGCACAG CATCACGGAG GAGATCGGCC ACGCTCAGGA GGCCAATCAG 
ACTTTGGTGG GCATCGACTG GCAGCATCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005156497.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156554.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

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CloneID	ODa18374
Clone ID Related Accession (Same CDS sequence)	XM_005156497.4 , XM_005156497.3
Accession Version	XM_005156497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG 
GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT 
GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA 
CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC 
TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT 
GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG 
ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC 
CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC 
GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG 
AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC 
CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT 
ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC 
GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC 
GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC 
ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA 
GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT 
CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC 
TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA 
TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT 
CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT 
GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA 
AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT 
ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC 
AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA 
GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT 
TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC 
AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA 
GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG 
CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG 
GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG 
TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT 
CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC 
AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG 
AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC 
TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC 
AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG 
GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC 
AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC 
ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC 
TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG 
CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT 
GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC 
ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG 
CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG 
CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA 
TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA 
CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT 
GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT 
GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC 
ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG 
CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC 
ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA 
TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC 
TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT 
CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT 
TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT 
GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC 
TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG 
GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG 
GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA 
GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT 
CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA 
CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT 
CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT 
CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG 
TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC 
ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC 
TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA 
AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG 
GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT 
GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC 
CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156554.1
CDS	70..5343
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005156497.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156497.4

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG 
GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT 
GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA 
CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC 
TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT 
GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG 
ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC 
CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC 
GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG 
AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC 
CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT 
ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC 
GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC 
GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC 
ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA 
GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT 
CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC 
TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA 
TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT 
CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT 
GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA 
AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT 
ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC 
AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA 
GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT 
TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC 
AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA 
GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG 
CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG 
GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG 
TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT 
CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC 
AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG 
AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC 
TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC 
AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG 
GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC 
AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC 
ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC 
TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG 
CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT 
GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC 
ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG 
CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG 
CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA 
TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA 
CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT 
GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT 
GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC 
ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG 
CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC 
ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA 
TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC 
TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT 
CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT 
TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT 
GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC 
TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG 
GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG 
GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA 
GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT 
CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA 
CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT 
CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT 
CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG 
TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC 
ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC 
TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA 
AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG 
GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT 
GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC 
CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005156497.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156554.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

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CloneID	ODa18374
Clone ID Related Accession (Same CDS sequence)	XM_005156497.4 , XM_005156497.3
Accession Version	XM_005156497.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG 
GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT 
GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA 
CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC 
TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT 
GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG 
ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC 
CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC 
GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG 
AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC 
CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT 
ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC 
GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC 
GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC 
ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA 
GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT 
CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC 
TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA 
TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT 
CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT 
GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA 
AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT 
ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC 
AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA 
GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT 
TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC 
AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA 
GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG 
CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG 
GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG 
TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT 
CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC 
AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG 
AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC 
TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC 
AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG 
GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC 
AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC 
ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC 
TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG 
CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT 
GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC 
ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG 
CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG 
CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA 
TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA 
CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT 
GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT 
GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC 
ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG 
CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC 
ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA 
TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC 
TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT 
CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT 
TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT 
GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC 
TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG 
GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG 
GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA 
GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT 
CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA 
CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT 
CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT 
CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG 
TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC 
ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC 
TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA 
AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG 
GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT 
GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC 
CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156554.1
CDS	67..5340
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSADALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005156497.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156497.3

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGCA GATGCTCTGA GGAGGATCTT GTGTCAGGTG 
GGTCTGCAGA GAGGACCGGA GGGAGAAAAC TCCTCTCTAG TGGACAAACT CATGCTCTGT 
GACTCCAAGA TGTGGAAAGG AGCGAGAAAT GTTTACCATC AGCTCTTTAT GAGCAGTCTA 
CTCATGGATC CGAAGTACAA GAAACTTTTT GCTATCCAGT TTGCGAGGAA CTATGAGCGC 
TTGCAGAGCG ACTATGTGAA GGACGACCAC GACAGGGAGT TCTCCGTCAC TGATCTTTCT 
GTGCAAATGT TCACCGTGCC TTCTCTGGCG CGGATGCTGA TCACAGATGA GAATCTGATG 
ACCACCATCA TCCGCACGTT CGTGGATCAC CTCAGACACC GAGACCTGCA GGGCCGCTTC 
CAGTTTGAAC GCTACACCGC ACAACAGGCC TTCAAATTCC GCCGAGTCCA GAGCCTTATC 
GGAGACCTCA AGTATGTCTT GATCAGCCGT CCAACAGAGT GGACCGACCA ACTCAGGGAG 
AAGTATCTGG AAGGTTTTGA TGCTTTCTTG GAGTTGCTTA AATGCATGCA GGGAATGGAC 
CCGGTTGTGC GCCAGGTGGG GCAGCACATA GAGATGGAGC CAGAATGGGA GGCAGCGTTT 
ACCTTACAGA TGAAGCTCAC ACATATCATC TCCATGATGC AGGAGTGGTG TGCCAGTGAC 
GAGAGAGTGC TGATTGAAGC GTATAAGAAG AGCCTGAGTG CACTGACTCA CTGCCACAGC 
GGTTTCACAG ACGGAGAGCA GCCTATAACC CTCAGCATGT GCGGTCACTC CGTCGACACC 
ATCCGCTACT GCGTCTCTCA GGAGAAAGTG AGCATACACC TGCCTGTGTC CCGGCTTCTA 
GCAGGTTTAC ATGCTCTGCT GAGCAAAATT GAGGTTGCCT ATCGATTCCC CGAGCAGCTT 
CCTCTGAGTG AACTAAGTCC ACCCATGCTG ATCGAACACC CGCTCCGCTG CCTTGTACTC 
TGTGCCCAGG TGCATGCTGG GATGTGGAGA AGAAACGGCT TCTCTTTGGT TAATCAGATA 
TATTATTATC ACAATGTGAA GTGTAGAGTG GAGATGTTTG ATAAAGACCT CATCATGCTT 
CAGGCTGGAG CTTCCATGAT GGATCCAAAC CACTTCCTGA TGATCATGTT GAGTCGTTTT 
GAGCTCTATC ATATCTTCAG CTCAGCAGAC TGCAGAAAGA GATACAGCCG AGAGAACGCA 
AATAAGGATG TGGTTCAGCA AAATAACACC CTAATAGAGG AAATGCTGCA CTTGATCATT 
ATGATCGTGG GTGAGCGCTA CACAGCAGGT GTCGGTCAGG TGGAGATCAC GGATGAGATC 
AGACGAGAGA TCGTTCACCA GCTCTGCATT CGACCCATGG CCCACAGCGA GCTGGTCAAA 
GCACTGCCTG AGAATGAGAA CAAGGAGACA GGAATGGAGA GGGTCATCGA TAGTGTGGCT 
TTGTTTAAGA AGCCTGGCGT GACGGGTAGA GGACTCTATA AACTCAGACC AGAGTGTGCC 
AAGATGTTCA ATCTTTACTT CCACCATTAC TCCAGAGCTG ACCAGTCTAA GGCTGAAGAA 
GCTCAAAGAA AAATAAAGAG GCAAAATGGG GAAGATTCAG TTCTGCCTCC ACCAGTCCTG 
CCTCGCTTCT GTCCGCTGTT TGCCAGTCTG GTGAACATCC TGCAGTGTGA CGTGCTGCTG 
GGCATTCTAG GAGCTGTTCT GCAGTGGGCA ATGGAGCCTA GTGGAGGACA CTGGTCTGAG 
TCCATGCTTC AGAGGGTGCT GCATCTCATT GGGATGGGGC TGGTGGAAGA ACAGCAGCAT 
CTCATCAGCA GCGCAGAAGA CTATAATGAT ACCAACTTCA ATTTCACTCT CAAAATCTCC 
AGACCCGGCG AGGCTCCTGC TAACACTGCC AGCATCTTGG CTTTACTGGA GACGCTGCAG 
AACGCTCCTC ATCTGGAGGT GCACAAGGAC ATGATCCGCT GGATCCTCAA GACTGTGGCC 
TACATCAAAA CCACGCGTGA GTGCACAGCC AGTGCGCCCC CCAGTGACAG CTCAGGGCAC 
AGTCAAGAGG AGACGGTGCG GGATAAGGAA AAGGCGGAGC GGAAGAGGAA AGCGGAGATG 
GCGCGACTCA GGAGAGAGAA AATCATGGCT CAGATGTCAG AGATGCAGAG ACACTTCATC 
AACGAGAACA AAGAGTTGTT CCAGCAGAGC CTGGAGGACC TGGACCCCTC CACATCCTCC 
ACGCTGGAGC ACAGCCCCAG CTCGTGTGAC AGTGCTCTGG TGTGTGTGGG CCCGCGGCGC 
TGGCGAGCAG GTGGCAGCGA CCGCAGGCAG GTGGTGACAT GTATCCTGTG CCAGGAGGAG 
CAGGAGATCC GTAGCGACGG CAAAGCCATG GTCCTCGCTG CATTCGTCCA GCGCTCCACT 
GTCATGTCCA AAAACCGCAA GAGACCACCA CACAACCCAG ATAAATATGA CCCCCTGTTC 
ATGCACCCCG ACCTGTCGTT CGGCACACAC ACGGGCAGCT GTGGACACAT CATGCACTCG 
CACTGCTGGC AGAGGTATTT TGAGGCGGTG CAGGCTAAAG AGCAGCGCAG GCAGCAGAGG 
CTCCGTGTGC ACACTAGTTA TGACGTGGAG AACGGAGAGT TTTTGTGTCC ACTCTGCGAA 
TGTCTGAGTA ACACAGTCAT TCCTCTGCTG CCACTCACTA AGACCTGCTG CAGCTCTGAA 
CAGCCTGATC TGAGCCAGTG GTTAAACATC ACCTCTCATC AGATGAAAGC CCTGATCTCT 
GCTCACAGGA AGTCCAAAAC CAGCAGTGCT GAAGCTGCTG ATGAAACAGA CGGTGTCAAT 
GACTGTCCAC CACCTGATGG CTTCAGACTA GATCACACTC CAAAAAATCC ATATTCAAGC 
ACCATAAAGG AGATGCTGAC TACGTTTGGG ACAGCCACGT ATAAAGTGGG TCTGAAAGTG 
CACCCGAATG AGCAGGACCC CCGAGTGCCA ATCATGTGCT GGGGCAGCTG TGCCTACACC 
ATCCAGTCTA TAGAGAGATT ACTGGTGGAT GAAGAGAAGC CTTTGTTTGG AAGTTTACCA 
TGCAGACAGG ACGACTGTTT GAGTTCTCTG ACGCGGTTTG GCTCTGCCTG CTGGACAGTC 
TCCTCTCATA CCACTGTACA GAATCACTTC ATCAGGCTCT TAGCAGCCCT TGTTCCTGAT 
CCCCGGGTGG AGAATACCCC TTGTATTCTG GATGTAGACA TGTTTCACTT GCTGGTGAGT 
TTGGTGCTGT CCCATCCAGC AGTTCTCTGT CTGGATTCCT CAGGGCTCAG CGCAGATGCT 
GCACAACTGC ACTTCTTGCA CCTGATCACA GTCGCTCACG TTGTCCAGAT CCTCCTGACC 
TCTTTACCTG AGGAGGTGCG GATGGAGCAG GAGACTGGAG GAGCCGAGAG AGAAGAGGAG 
GAGAGTGTGT GTTTGCTCTA CAATACTCTC AGGAGTCTGC TGGGCCGTGG CTTGCGCGAG 
GTGAGCTCAG GCTGGCATCT GTGGCGCTGT GTGAAAGCTG GAATCCTGCC TTATCTGAGA 
GCCGCCGCTC TCTTCTTCCA CTATCTCAAC GGAGCTCCAG CACCACCGGA GTTACACGCT 
CTAGGTGCGG GTGAATGGGA GGCTCTGTGT GGATACCTGT GTCTGCCTTC CAACCTGCTA 
CAGCTCTACT TCAACAATCT AGACATCATG GAGCCTCTGT TGCATGGGTG GTGTTCTCAT 
CCAGCTCTTC AGGCTCTTCA GAGCAGCGTG ACTCTCATCA GGTTTCCCAG AGAGTCCAAT 
CATCTCATCG ATCTGCCGGG CGACTACAGC GCGTTGATCA ACCAGGCCTC CAGCTTCACG 
TGTCCTAAAT CTGGAGGAGA TAAGTCTCGC GTTCCTACTC TGTGTCTGGT GTGCGGTGCC 
ATGCTCTGCT CTCAGAGCTA CTGCTGTCAG ACGGAGCTGG AAGGAGAAGA CGTGGGGGCC 
TGTACCGCAC ACACTTTCAC CTGCGGAGCC GGAGTCGGCA TCTTCCTCAG GGTGAGGGAA 
AGTCAGGTCC TGTTCCTGGC TGGCAAAACT AAGGGCTGTT TTTATGCTCC CCCATACCTG 
GATGACTATG GAGAAACAGA CCAAGGGCTA AGACGGGGGA ACCCATTACA CTTGTGCCAT 
GAGCGCTACA GAAAGATCCA GAAGCTGTGG CGTCAGCACA GCATCACGGA GGAGATCGGC 
CACGCTCAGG AGGCCAATCA GACTTTGGTG GGCATCGACT GGCAGCATCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005156498.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156555.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

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CloneID	ODa18375
Clone ID Related Accession (Same CDS sequence)	XM_005156498.4 , XM_005156498.3
Accession Version	XM_005156498.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG 
CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG 
CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156555.1
CDS	69..5339
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005156498.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156498.4

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG 
CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG 
CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_005156498.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156555.1 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

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CloneID	ODa18375
Clone ID Related Accession (Same CDS sequence)	XM_005156498.4 , XM_005156498.3
Accession Version	XM_005156498.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG 
CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG 
CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156555.1
CDS	67..5337
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARNYERLQSDYVKDDHDREFSVTDLSVQMFTVPSLARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_005156498.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156498.3

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAACTA TGAGCGCTTG 
CAGAGCGACT ATGTGAAGGA CGACCACGAC AGGGAGTTCT CCGTCACTGA TCTTTCTGTG 
CAAATGTTCA CCGTGCCTTC TCTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_001921555.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001921590.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

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CloneID	ODa18376
Clone ID Related Accession (Same CDS sequence)	XM_001921555.7 , XM_001921555.6
Accession Version	XM_001921555.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921555.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT 
CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG 
CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921590.3
CDS	70..5340
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARSYQRLQTDFMEDDHERVVSVTSLSVQLFTAPTVARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_001921555.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921555.7

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT 
CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG 
CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR2 ( XM_001921555.6 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001921590.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

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CloneID	ODa18376
Clone ID Related Accession (Same CDS sequence)	XM_001921555.7 , XM_001921555.6
Accession Version	XM_001921555.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase UBR2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921555.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT 
CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG 
CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921590.3
CDS	67..5337
Translation	MAAAETDRDAPSALCSEFLNFSAKDTAAKWLQVSNLPKEVYQHLACYVPKIYCLGPNLNPQNEDLLAQLLLQGPLEWYLCGEEPSVGLSKLEQNNQPSQLCGHVFKVGEPTYSCRECAADPTCVLCMQCFLGSVHKEHRYRMTTSGGGGFCDCGDTEAWKKGPYCQKHEPNISDSSQKDPLANLSAEMIARTYNVFSIILKYAVDMLTWDKEDELPEGLEPPARGDMYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAVSFATTVDRDGRKSVRFGDFQFCEQAKSVIVRNTSRQTKPLRVQVMHSSVVAHQCFALKALNWLGQTIRYSDALRRILCQVGLQRGPEGENSSLVDKLMLCDSKMWKGARNVYHQLFMSSLLMDPKYKKLFAIQFARSYQRLQTDFMEDDHERVVSVTSLSVQLFTAPTVARMLITDENLMTTIIRTFVDHLRHRDLQGRFQFERYTAQQAFKFRRVQSLIGDLKYVLISRPTEWTDQLREKYLEGFDAFLELLKCMQGMDPVVRQVGQHIEMEPEWEAAFTLQMKLTHIISMMQEWCASDERVLIEAYKKSLSALTHCHSGFTDGEQPITLSMCGHSVDTIRYCVSQEKVSIHLPVSRLLAGLHALLSKIEVAYRFPEQLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRVEMFDKDLIMLQAGASMMDPNHFLMIMLSRFELYHIFSSADCRKRYSRENANKDVVQQNNTLIEEMLHLIIMIVGERYTAGVGQVEITDEIRREIVHQLCIRPMAHSELVKALPENENKETGMERVIDSVALFKKPGVTGRGLYKLRPECAKMFNLYFHHYSRADQSKAEEAQRKIKRQNGEDSVLPPPVLPRFCPLFASLVNILQCDVLLGILGAVLQWAMEPSGGHWSESMLQRVLHLIGMGLVEEQQHLISSAEDYNDTNFNFTLKISRPGEAPANTASILALLETLQNAPHLEVHKDMIRWILKTVAYIKTTRECTASAPPSDSSGHSQEETVRDKEKAERKRKAEMARLRREKIMAQMSEMQRHFINENKELFQQSLEDLDPSTSSTLEHSPSSCDSALVCVGPRRWRAGGSDRRQVVTCILCQEEQEIRSDGKAMVLAAFVQRSTVMSKNRKRPPHNPDKYDPLFMHPDLSFGTHTGSCGHIMHSHCWQRYFEAVQAKEQRRQQRLRVHTSYDVENGEFLCPLCECLSNTVIPLLPLTKTCCSSEQPDLSQWLNITSHQMKALISAHRKSKTSSAEAADETDGVNDCPPPDGFRLDHTPKNPYSSTIKEMLTTFGTATYKVGLKVHPNEQDPRVPIMCWGSCAYTIQSIERLLVDEEKPLFGSLPCRQDDCLSSLTRFGSACWTVSSHTTVQNHFIRLLAALVPDPRVENTPCILDVDMFHLLVSLVLSHPAVLCLDSSGLSADAAQLHFLHLITVAHVVQILLTSLPEEVRMEQETGGAEREEEESVCLLYNTLRSLLGRGLREVSSGWHLWRCVKAGILPYLRAAALFFHYLNGAPAPPELHALGAGEWEALCGYLCLPSNLLQLYFNNLDIMEPLLHGWCSHPALQALQSSVTLIRFPRESNHLIDLPGDYSALINQASSFTCPKSGGDKSRVPTLCLVCGAMLCSQSYCCQTELEGEDVGACTAHTFTCGAGVGIFLRVRESQVLFLAGKTKGCFYAPPYLDDYGETDQGLRRGNPLHLCHERYRKIQKLWRQHSITEEIGHAQEANQTLVGIDWQHL

Target ORF information:

RefSeq Version	XM_001921555.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin protein ligase E3 component n-recognin 2 (ubr2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921555.6

ORF Insert Sequence:

ATGGCGGCGG CCGAGACCGA TCGTGATGCA CCGTCCGCTC TGTGTAGCGA ATTCCTCAAT 
TTCTCCGCTA AGGACACCGC TGCGAAATGG CTACAGGTGA GCAACCTACC CAAAGAGGTG 
TACCAGCACT TGGCCTGCTA TGTCCCCAAG ATCTACTGCC TGGGTCCCAA CCTCAACCCT 
CAGAACGAGG ACCTGCTTGC TCAGCTGCTG CTTCAGGGCC CGCTGGAGTG GTATCTGTGT 
GGGGAAGAGC CGTCCGTGGG CTTGTCCAAA CTCGAACAGA ACAACCAGCC GTCACAACTT 
TGTGGCCATG TGTTTAAAGT GGGAGAGCCA ACATACTCCT GCAGGGAGTG TGCAGCCGAT 
CCGACCTGTG TGCTGTGTAT GCAGTGCTTC TTAGGCAGCG TCCACAAAGA GCATCGCTAC 
AGGATGACAA CCTCAGGAGG AGGAGGCTTC TGCGACTGTG GAGACACAGA GGCCTGGAAG 
AAGGGTCCTT ACTGTCAAAA ACACGAGCCT AATATCAGTG ACTCCTCCCA GAAGGATCCT 
TTAGCGAATC TGTCTGCTGA GATGATCGCT CGCACGTATA ATGTTTTCTC CATCATCCTC 
AAATATGCAG TGGACATGCT CACCTGGGAC AAAGAGGACG AGCTGCCTGA AGGCCTCGAG 
CCACCGGCAC GTGGAGATAT GTACTACTGC ATGCTGTTCA ACGATGAGGT CCACACGTAC 
GAGCAGGTCA TCTATACACT ACAGAAAGCT GTGAACTGCA CCCAGAAAGA GGCCGTCAGT 
TTCGCCACCA CCGTGGACAG AGATGGCAGG AAGTCTGTTC GCTTTGGGGA CTTCCAGTTT 
TGCGAGCAGG CCAAATCTGT TATTGTGAGA AACACGAGTC GGCAGACGAA ACCTCTGCGG 
GTTCAGGTCA TGCACTCTTC TGTAGTGGCT CATCAGTGTT TTGCACTCAA AGCTCTGAAC 
TGGCTTGGCC AAACCATCAG ATACTCAGAT GCTCTGAGGA GGATCTTGTG TCAGGTGGGT 
CTGCAGAGAG GACCGGAGGG AGAAAACTCC TCTCTAGTGG ACAAACTCAT GCTCTGTGAC 
TCCAAGATGT GGAAAGGAGC GAGAAATGTT TACCATCAGC TCTTTATGAG CAGTCTACTC 
ATGGATCCGA AGTACAAGAA ACTTTTTGCT ATCCAGTTTG CGAGGAGTTA CCAGCGCCTT 
CAGACAGATT TTATGGAGGA CGATCACGAG CGTGTAGTGT CAGTGACCTC TCTGTCAGTG 
CAGCTCTTCA CCGCGCCTAC TGTGGCGCGG ATGCTGATCA CAGATGAGAA TCTGATGACC 
ACCATCATCC GCACGTTCGT GGATCACCTC AGACACCGAG ACCTGCAGGG CCGCTTCCAG 
TTTGAACGCT ACACCGCACA ACAGGCCTTC AAATTCCGCC GAGTCCAGAG CCTTATCGGA 
GACCTCAAGT ATGTCTTGAT CAGCCGTCCA ACAGAGTGGA CCGACCAACT CAGGGAGAAG 
TATCTGGAAG GTTTTGATGC TTTCTTGGAG TTGCTTAAAT GCATGCAGGG AATGGACCCG 
GTTGTGCGCC AGGTGGGGCA GCACATAGAG ATGGAGCCAG AATGGGAGGC AGCGTTTACC 
TTACAGATGA AGCTCACACA TATCATCTCC ATGATGCAGG AGTGGTGTGC CAGTGACGAG 
AGAGTGCTGA TTGAAGCGTA TAAGAAGAGC CTGAGTGCAC TGACTCACTG CCACAGCGGT 
TTCACAGACG GAGAGCAGCC TATAACCCTC AGCATGTGCG GTCACTCCGT CGACACCATC 
CGCTACTGCG TCTCTCAGGA GAAAGTGAGC ATACACCTGC CTGTGTCCCG GCTTCTAGCA 
GGTTTACATG CTCTGCTGAG CAAAATTGAG GTTGCCTATC GATTCCCCGA GCAGCTTCCT 
CTGAGTGAAC TAAGTCCACC CATGCTGATC GAACACCCGC TCCGCTGCCT TGTACTCTGT 
GCCCAGGTGC ATGCTGGGAT GTGGAGAAGA AACGGCTTCT CTTTGGTTAA TCAGATATAT 
TATTATCACA ATGTGAAGTG TAGAGTGGAG ATGTTTGATA AAGACCTCAT CATGCTTCAG 
GCTGGAGCTT CCATGATGGA TCCAAACCAC TTCCTGATGA TCATGTTGAG TCGTTTTGAG 
CTCTATCATA TCTTCAGCTC AGCAGACTGC AGAAAGAGAT ACAGCCGAGA GAACGCAAAT 
AAGGATGTGG TTCAGCAAAA TAACACCCTA ATAGAGGAAA TGCTGCACTT GATCATTATG 
ATCGTGGGTG AGCGCTACAC AGCAGGTGTC GGTCAGGTGG AGATCACGGA TGAGATCAGA 
CGAGAGATCG TTCACCAGCT CTGCATTCGA CCCATGGCCC ACAGCGAGCT GGTCAAAGCA 
CTGCCTGAGA ATGAGAACAA GGAGACAGGA ATGGAGAGGG TCATCGATAG TGTGGCTTTG 
TTTAAGAAGC CTGGCGTGAC GGGTAGAGGA CTCTATAAAC TCAGACCAGA GTGTGCCAAG 
ATGTTCAATC TTTACTTCCA CCATTACTCC AGAGCTGACC AGTCTAAGGC TGAAGAAGCT 
CAAAGAAAAA TAAAGAGGCA AAATGGGGAA GATTCAGTTC TGCCTCCACC AGTCCTGCCT 
CGCTTCTGTC CGCTGTTTGC CAGTCTGGTG AACATCCTGC AGTGTGACGT GCTGCTGGGC 
ATTCTAGGAG CTGTTCTGCA GTGGGCAATG GAGCCTAGTG GAGGACACTG GTCTGAGTCC 
ATGCTTCAGA GGGTGCTGCA TCTCATTGGG ATGGGGCTGG TGGAAGAACA GCAGCATCTC 
ATCAGCAGCG CAGAAGACTA TAATGATACC AACTTCAATT TCACTCTCAA AATCTCCAGA 
CCCGGCGAGG CTCCTGCTAA CACTGCCAGC ATCTTGGCTT TACTGGAGAC GCTGCAGAAC 
GCTCCTCATC TGGAGGTGCA CAAGGACATG ATCCGCTGGA TCCTCAAGAC TGTGGCCTAC 
ATCAAAACCA CGCGTGAGTG CACAGCCAGT GCGCCCCCCA GTGACAGCTC AGGGCACAGT 
CAAGAGGAGA CGGTGCGGGA TAAGGAAAAG GCGGAGCGGA AGAGGAAAGC GGAGATGGCG 
CGACTCAGGA GAGAGAAAAT CATGGCTCAG ATGTCAGAGA TGCAGAGACA CTTCATCAAC 
GAGAACAAAG AGTTGTTCCA GCAGAGCCTG GAGGACCTGG ACCCCTCCAC ATCCTCCACG 
CTGGAGCACA GCCCCAGCTC GTGTGACAGT GCTCTGGTGT GTGTGGGCCC GCGGCGCTGG 
CGAGCAGGTG GCAGCGACCG CAGGCAGGTG GTGACATGTA TCCTGTGCCA GGAGGAGCAG 
GAGATCCGTA GCGACGGCAA AGCCATGGTC CTCGCTGCAT TCGTCCAGCG CTCCACTGTC 
ATGTCCAAAA ACCGCAAGAG ACCACCACAC AACCCAGATA AATATGACCC CCTGTTCATG 
CACCCCGACC TGTCGTTCGG CACACACACG GGCAGCTGTG GACACATCAT GCACTCGCAC 
TGCTGGCAGA GGTATTTTGA GGCGGTGCAG GCTAAAGAGC AGCGCAGGCA GCAGAGGCTC 
CGTGTGCACA CTAGTTATGA CGTGGAGAAC GGAGAGTTTT TGTGTCCACT CTGCGAATGT 
CTGAGTAACA CAGTCATTCC TCTGCTGCCA CTCACTAAGA CCTGCTGCAG CTCTGAACAG 
CCTGATCTGA GCCAGTGGTT AAACATCACC TCTCATCAGA TGAAAGCCCT GATCTCTGCT 
CACAGGAAGT CCAAAACCAG CAGTGCTGAA GCTGCTGATG AAACAGACGG TGTCAATGAC 
TGTCCACCAC CTGATGGCTT CAGACTAGAT CACACTCCAA AAAATCCATA TTCAAGCACC 
ATAAAGGAGA TGCTGACTAC GTTTGGGACA GCCACGTATA AAGTGGGTCT GAAAGTGCAC 
CCGAATGAGC AGGACCCCCG AGTGCCAATC ATGTGCTGGG GCAGCTGTGC CTACACCATC 
CAGTCTATAG AGAGATTACT GGTGGATGAA GAGAAGCCTT TGTTTGGAAG TTTACCATGC 
AGACAGGACG ACTGTTTGAG TTCTCTGACG CGGTTTGGCT CTGCCTGCTG GACAGTCTCC 
TCTCATACCA CTGTACAGAA TCACTTCATC AGGCTCTTAG CAGCCCTTGT TCCTGATCCC 
CGGGTGGAGA ATACCCCTTG TATTCTGGAT GTAGACATGT TTCACTTGCT GGTGAGTTTG 
GTGCTGTCCC ATCCAGCAGT TCTCTGTCTG GATTCCTCAG GGCTCAGCGC AGATGCTGCA 
CAACTGCACT TCTTGCACCT GATCACAGTC GCTCACGTTG TCCAGATCCT CCTGACCTCT 
TTACCTGAGG AGGTGCGGAT GGAGCAGGAG ACTGGAGGAG CCGAGAGAGA AGAGGAGGAG 
AGTGTGTGTT TGCTCTACAA TACTCTCAGG AGTCTGCTGG GCCGTGGCTT GCGCGAGGTG 
AGCTCAGGCT GGCATCTGTG GCGCTGTGTG AAAGCTGGAA TCCTGCCTTA TCTGAGAGCC 
GCCGCTCTCT TCTTCCACTA TCTCAACGGA GCTCCAGCAC CACCGGAGTT ACACGCTCTA 
GGTGCGGGTG AATGGGAGGC TCTGTGTGGA TACCTGTGTC TGCCTTCCAA CCTGCTACAG 
CTCTACTTCA ACAATCTAGA CATCATGGAG CCTCTGTTGC ATGGGTGGTG TTCTCATCCA 
GCTCTTCAGG CTCTTCAGAG CAGCGTGACT CTCATCAGGT TTCCCAGAGA GTCCAATCAT 
CTCATCGATC TGCCGGGCGA CTACAGCGCG TTGATCAACC AGGCCTCCAG CTTCACGTGT 
CCTAAATCTG GAGGAGATAA GTCTCGCGTT CCTACTCTGT GTCTGGTGTG CGGTGCCATG 
CTCTGCTCTC AGAGCTACTG CTGTCAGACG GAGCTGGAAG GAGAAGACGT GGGGGCCTGT 
ACCGCACACA CTTTCACCTG CGGAGCCGGA GTCGGCATCT TCCTCAGGGT GAGGGAAAGT 
CAGGTCCTGT TCCTGGCTGG CAAAACTAAG GGCTGTTTTT ATGCTCCCCC ATACCTGGAT 
GACTATGGAG AAACAGACCA AGGGCTAAGA CGGGGGAACC CATTACACTT GTGCCATGAG 
CGCTACAGAA AGATCCAGAA GCTGTGGCGT CAGCACAGCA TCACGGAGGA GATCGGCCAC 
GCTCAGGAGG CCAATCAGAC TTTGGTGGGC ATCGACTGGC AGCATCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UBR2
Entrez Gene ID	559136
Full Name	ubiquitin protein ligase E3 component n-recognin 2
Synonyms	si:ch211-239i4.2
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 2 Names E3 ubiquitin-protein ligase UBR2
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Rattus norvegicus (Norway rat)	Ubr2	NP_001171542.1
Gallus gallus (chicken)	UBR2	XP_004935398.1
Drosophila melanogaster (fruit fly)	CG9086	NP_001245723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000171	XP_310967.5
Caenorhabditis elegans (roundworm)	ubr-1	NP_491231.2
Xenopus tropicalis (tropical clawed frog)	ubr2	XP_002934736.1
	UBR2	XP_001088074.2
Bos taurus (cattle)	UBR2	NP_001179567.1
Danio rerio (zebrafish)	ubr2	XP_005156554.1
Mus musculus (house mouse)	Ubr2	NP_001170845.1
Homo sapiens (human)	UBR2	NP_056070.1
Pan troglodytes (chimpanzee)	UBR2	XP_001134707.1
Canis lupus familiaris (dog)	UBR2	XP_005627416.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UBR2 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

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