PPEF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPEF1
Entrez Gene ID	559132
Full Name	protein phosphatase, EF-hand calcium binding domain 1
Synonyms	zgc:171580
General protein information	Preferred Names protein phosphatase, EF-hand calcium binding domain 1 Names serine/threonine-protein phosphatase with EF-hands 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114730.1	NP_001108202.1	serine/threonine-protein phosphatase with EF-hands 1
XM_021479632.1	XP_021335307.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X2
XM_017358234.1	XP_017213723.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X2
XM_021479631.1	XP_021335306.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
NM_001114730.2	NP_001108202.2	serine/threonine-protein phosphatase with EF-hands 1
XM_009306114.2	XP_009304389.1	serine/threonine-protein phosphatase with EF-hands 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PPEF1	XP_003953993.1
Caenorhabditis elegans (roundworm)	pef-1	NP_001254836.1
	PPEF1	XP_001083653.1
Bos taurus (cattle)	PPEF1	XP_005228463.1
Gallus gallus (chicken)	PPEF1	XP_003640538.2
Canis lupus familiaris (dog)	PPEF1	XP_003640299.2
Homo sapiens (human)	PPEF1	NP_006231.2
Danio rerio (zebrafish)	ppef1	NP_001108202.1
Mus musculus (house mouse)	Ppef1	NP_035277.1
Rattus norvegicus (Norway rat)	Ppef1	NP_001030107.1
Xenopus tropicalis (tropical clawed frog)	ppef1	XP_002936093.2

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17192	NM_001114730.2 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa35238	NM_001114730.1 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17191	XM_021479631.1 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17191	XM_009306114.2 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17192	XM_021479632.1 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17192	XM_017358234.1 Latest version!	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPEF1 ( NM_001114730.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001108202.2 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.

Price & Availability↑

CloneID	ODa17192
Clone ID Related Accession (Same CDS sequence)	XM_021479632.1 , XM_017358234.1 , NM_001114730.2
Accession Version	NM_001114730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR790368.17. On or before Apr 21, 2018 this sequence version replaced XM_021479632.1, NM_001114730.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC159114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108202.2
CDS	295..2451
Translation	MGCGTSIAIENQTRPSETAIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQINGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTPRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDINNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	NM_001114730.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114730.2

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( NM_001114730.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001108202.1 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.

Price & Availability↑

CloneID	ODa35238
Clone ID Related Accession (Same CDS sequence)	NM_001114730.1
Accession Version	NM_001114730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC159114.1. On Feb 22, 2008 this sequence version replaced XM_682447.2. ##Evidence-Data-START## Transcript exon combination :: BC159114.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATGAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTTCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAGATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAGATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TACACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCGTT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGCG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCGA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATAT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108202.1
CDS	295..2451
Translation	MGCGTSIAIENQTRPSETAIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQMNGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTTRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDIDNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	NM_001114730.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114730.1

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATGAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTTCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAGATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAGATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TACACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCGTT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGCG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCGA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATAT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_021479631.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335306.1 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17191
Clone ID Related Accession (Same CDS sequence)	XM_021479631.1 , XM_009306114.2
Accession Version	XM_021479631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGATGGC 
TCCATCAAGC AGTCCAACCC AGCTCTGACC ATAAAAGCTG CCATACTGAT CCAGCGTTGG 
TACAGACGCT ATGTTGCTCA CCTGGAGATG CGAAGACGCT ACACGTGGAA CATCTTTCAG 
TCCATTGAGT ATGCAGGAGA ACAGGACCAG CTACAGCTCT CAAGTTTCTT CACTTTCATG 
TTGGACAACT TCACTCAGAT TAATGGGAAT GGACCAGACT TGATCTCCCA CCTGCTGGAC 
TCTGTGGTTG ATCCTCTGAC GGAGGAGGTC AGGCAGCTGA AGTACGAGCA GATTATGGTG 
CCGGAGTCGT ACAGCGGCCC TCGTCTCTCC TTCCCGCTGA GCGTGACTGA TACCAATGCC 
CTGCTCAGTG CCTTTAAAGA GGAGCAGACT CTTCATGGGA AGTATGTGCT GCAGTTACTG 
TATGAAACCA AGAAGTTCCT GAAACAAATG CCAAACGTCA TTTATCTGTC AACGTCCTAT 
GCCAAAGAAA TAACGATCTG TGGCGATCTG CATGGAAGAC TGGATGACTT GCTGCTTATA 
TTTTACAAGA ACGGTCTGCC CTCTGCGGAA AACCCCTACA TATTCAATGG AGACTTTGTG 
GATCGAGGAA AGAAATCTAC AGAGATTATC ATCATTCTCT TTGCCTTCGT TTTGCTCTAC 
CCCGACCACA TGCATCTGAA CAGGGGAAAC CATGAGGACC ACATCATGAA CCTCAGATAT 
GGATTCACCA GAGAAGTCAT GCAGAAGTAC AAGACTCATG GCAGGGAAAT CCTGCAGCTC 
CTTCAGGATG TGTTCAGTCT TCTTCCTGTT GCAACTGTAA TAGACAACAA AGTGCTTATT 
GTTCATGGTG GCATATCGGA CAAAACTGAT CTGGACTTTC TGAGCACTGT TGAAAGGCAT 
AAAGTGAAGT CAGCTCTGCG TTTGCCGAAG CGCAGCACTG ATCATCTGAC GGTGCGCTCG 
TGCAGTCGCA GCAGCAGCAG AAACAGGGGC AGAAACAGCA GATCCTCAAG CCGACACTCG 
TCCCGCAGCA GAAGGAAGAA GCTCAGCAAG CAGGGCAGCC GCTCCTCCTC TTCCTCATCC 
TCCTCATCCT CCTCTTCCTC CATGTGTTCG CCACGGGTCT CAGCCAAAAA CACTCCACGC 
CGCCCTCCAC CGAGCCCCAC AGACATGCTC CAAGTGCCCT TTCTGGACTC ATTCGTGTCC 
TTGGAGCCAC CAGAGCTGCC CAGAGAAGAG CTGGAGTGGC AGCAGGTTGT GGATATCCTG 
TGGAGTGACC CTAAACATCA GAATGGCTGC AGTCCCAATT CTTTTCGCGG TGGAGGCTGT 
TACTTCGGGC CAGACGTGAC ACAGACACTG CTGCAGAAAC ACGGCCTCTG TCTGCTCATC 
AGATCACACG AATGCAAACA GGAGGGCTAT GAGCTTTGCC ACAATGGACA GGTTATCACT 
ATATTCTCTG CATCAAACTA CTATGAAGAG GGCAGTAACC GTGGGGCTTA TGTCAAACTG 
GGGCCTGAAC TTGTCCCTCG CTTCTTCCAG TATCAAGTCA GTCGAAGCAC CAGAAAACTC 
ACCCTCCATC AGAGGGTTCG AGTAGCTGAA GGTTCTGCAC TCAGGGCTCT GAAAGAGAAA 
CTTTATGCTC ATCGCTCTGA ACTCATGGCT GCCTTTCAAC AGTATGACAT CAATAACACA 
GGCCGTATCT CCACTAGTGA GTGGGCACAG GTGGTGGAGT CTGTTCTGCG TTTGGATCTG 
CCATGGAGAA CCCTGCGCCC ACGCCTGGCC CGTCTTGGCA CAGATGGCAA TGTGGAATAT 
GAATCCTGTT TCGAGGACAT CAGTCCTGGA GAGCCCATCC CTCCGGTGAC CCCGAACTTA 
GCTGAAACAC TTTATAGATA TAGAACTGAC CTGGAGATCA TTTTTAACAT CATCGATAAG 
GATCACTCAG GTCAGATCTC CATCGAGGAG TTTCGTCAGA CCTGGAAGCT CTTCAGCTCT 
CATTTAGGGG TGGCTGTGAA TGACCAGACC ATAGACGACA TGGCTCGAAG CATAGACTTT 
AACAAAGACG GCAGCATTGA TTTTACTGAG TTCCTGGAGG CCTTCAGGGT GGTGCATAAA 
CTGGATGTCA AAGAGCAACA GCAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335306.1
CDS	465..2654
Translation	MGCGTSIAIENQTRPSETDGSIKQSNPALTIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQINGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTPRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDINNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	XM_021479631.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479631.1

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGATGGC 
TCCATCAAGC AGTCCAACCC AGCTCTGACC ATAAAAGCTG CCATACTGAT CCAGCGTTGG 
TACAGACGCT ATGTTGCTCA CCTGGAGATG CGAAGACGCT ACACGTGGAA CATCTTTCAG 
TCCATTGAGT ATGCAGGAGA ACAGGACCAG CTACAGCTCT CAAGTTTCTT CACTTTCATG 
TTGGACAACT TCACTCAGAT TAATGGGAAT GGACCAGACT TGATCTCCCA CCTGCTGGAC 
TCTGTGGTTG ATCCTCTGAC GGAGGAGGTC AGGCAGCTGA AGTACGAGCA GATTATGGTG 
CCGGAGTCGT ACAGCGGCCC TCGTCTCTCC TTCCCGCTGA GCGTGACTGA TACCAATGCC 
CTGCTCAGTG CCTTTAAAGA GGAGCAGACT CTTCATGGGA AGTATGTGCT GCAGTTACTG 
TATGAAACCA AGAAGTTCCT GAAACAAATG CCAAACGTCA TTTATCTGTC AACGTCCTAT 
GCCAAAGAAA TAACGATCTG TGGCGATCTG CATGGAAGAC TGGATGACTT GCTGCTTATA 
TTTTACAAGA ACGGTCTGCC CTCTGCGGAA AACCCCTACA TATTCAATGG AGACTTTGTG 
GATCGAGGAA AGAAATCTAC AGAGATTATC ATCATTCTCT TTGCCTTCGT TTTGCTCTAC 
CCCGACCACA TGCATCTGAA CAGGGGAAAC CATGAGGACC ACATCATGAA CCTCAGATAT 
GGATTCACCA GAGAAGTCAT GCAGAAGTAC AAGACTCATG GCAGGGAAAT CCTGCAGCTC 
CTTCAGGATG TGTTCAGTCT TCTTCCTGTT GCAACTGTAA TAGACAACAA AGTGCTTATT 
GTTCATGGTG GCATATCGGA CAAAACTGAT CTGGACTTTC TGAGCACTGT TGAAAGGCAT 
AAAGTGAAGT CAGCTCTGCG TTTGCCGAAG CGCAGCACTG ATCATCTGAC GGTGCGCTCG 
TGCAGTCGCA GCAGCAGCAG AAACAGGGGC AGAAACAGCA GATCCTCAAG CCGACACTCG 
TCCCGCAGCA GAAGGAAGAA GCTCAGCAAG CAGGGCAGCC GCTCCTCCTC TTCCTCATCC 
TCCTCATCCT CCTCTTCCTC CATGTGTTCG CCACGGGTCT CAGCCAAAAA CACTCCACGC 
CGCCCTCCAC CGAGCCCCAC AGACATGCTC CAAGTGCCCT TTCTGGACTC ATTCGTGTCC 
TTGGAGCCAC CAGAGCTGCC CAGAGAAGAG CTGGAGTGGC AGCAGGTTGT GGATATCCTG 
TGGAGTGACC CTAAACATCA GAATGGCTGC AGTCCCAATT CTTTTCGCGG TGGAGGCTGT 
TACTTCGGGC CAGACGTGAC ACAGACACTG CTGCAGAAAC ACGGCCTCTG TCTGCTCATC 
AGATCACACG AATGCAAACA GGAGGGCTAT GAGCTTTGCC ACAATGGACA GGTTATCACT 
ATATTCTCTG CATCAAACTA CTATGAAGAG GGCAGTAACC GTGGGGCTTA TGTCAAACTG 
GGGCCTGAAC TTGTCCCTCG CTTCTTCCAG TATCAAGTCA GTCGAAGCAC CAGAAAACTC 
ACCCTCCATC AGAGGGTTCG AGTAGCTGAA GGTTCTGCAC TCAGGGCTCT GAAAGAGAAA 
CTTTATGCTC ATCGCTCTGA ACTCATGGCT GCCTTTCAAC AGTATGACAT CAATAACACA 
GGCCGTATCT CCACTAGTGA GTGGGCACAG GTGGTGGAGT CTGTTCTGCG TTTGGATCTG 
CCATGGAGAA CCCTGCGCCC ACGCCTGGCC CGTCTTGGCA CAGATGGCAA TGTGGAATAT 
GAATCCTGTT TCGAGGACAT CAGTCCTGGA GAGCCCATCC CTCCGGTGAC CCCGAACTTA 
GCTGAAACAC TTTATAGATA TAGAACTGAC CTGGAGATCA TTTTTAACAT CATCGATAAG 
GATCACTCAG GTCAGATCTC CATCGAGGAG TTTCGTCAGA CCTGGAAGCT CTTCAGCTCT 
CATTTAGGGG TGGCTGTGAA TGACCAGACC ATAGACGACA TGGCTCGAAG CATAGACTTT 
AACAAAGACG GCAGCATTGA TTTTACTGAG TTCCTGGAGG CCTTCAGGGT GGTGCATAAA 
CTGGATGTCA AAGAGCAACA GCAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_009306114.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304389.1 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17191
Clone ID Related Accession (Same CDS sequence)	XM_021479631.1 , XM_009306114.2
Accession Version	XM_009306114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGATGGC 
TCCATCAAGC AGTCCAACCC AGCTCTGACC ATAAAAGCTG CCATACTGAT CCAGCGTTGG 
TACAGACGCT ATGTTGCTCA CCTGGAGATG CGAAGACGCT ACACGTGGAA CATCTTTCAG 
TCCATTGAGT ATGCAGGAGA ACAGGACCAG CTACAGCTCT CAAGTTTCTT CACTTTCATG 
TTGGACAACT TCACTCAGAT TAATGGGAAT GGACCAGACT TGATCTCCCA CCTGCTGGAC 
TCTGTGGTTG ATCCTCTGAC GGAGGAGGTC AGGCAGCTGA AGTACGAGCA GATTATGGTG 
CCGGAGTCGT ACAGCGGCCC TCGTCTCTCC TTCCCGCTGA GCGTGACTGA TACCAATGCC 
CTGCTCAGTG CCTTTAAAGA GGAGCAGACT CTTCATGGGA AGTATGTGCT GCAGTTACTG 
TATGAAACCA AGAAGTTCCT GAAACAAATG CCAAACGTCA TTTATCTGTC AACGTCCTAT 
GCCAAAGAAA TAACGATCTG TGGCGATCTG CATGGAAGAC TGGATGACTT GCTGCTTATA 
TTTTACAAGA ACGGTCTGCC CTCTGCGGAA AACCCCTACA TATTCAATGG AGACTTTGTG 
GATCGAGGAA AGAAATCTAC AGAGATTATC ATCATTCTCT TTGCCTTCGT TTTGCTCTAC 
CCCGACCACA TGCATCTGAA CAGGGGAAAC CATGAGGACC ACATCATGAA CCTCAGATAT 
GGATTCACCA GAGAAGTCAT GCAGAAGTAC AAGACTCATG GCAGGGAAAT CCTGCAGCTC 
CTTCAGGATG TGTTCAGTCT TCTTCCTGTT GCAACTGTAA TAGACAACAA AGTGCTTATT 
GTTCATGGTG GCATATCGGA CAAAACTGAT CTGGACTTTC TGAGCACTGT TGAAAGGCAT 
AAAGTGAAGT CAGCTCTGCG TTTGCCGAAG CGCAGCACTG ATCATCTGAC GGTGCGCTCG 
TGCAGTCGCA GCAGCAGCAG AAACAGGGGC AGAAACAGCA GATCCTCAAG CCGACACTCG 
TCCCGCAGCA GAAGGAAGAA GCTCAGCAAG CAGGGCAGCC GCTCCTCCTC TTCCTCATCC 
TCCTCATCCT CCTCTTCCTC CATGTGTTCG CCACGGGTCT CAGCCAAAAA CACTCCACGC 
CGCCCTCCAC CGAGCCCCAC AGACATGCTC CAAGTGCCCT TTCTGGACTC ATTCGTGTCC 
TTGGAGCCAC CAGAGCTGCC CAGAGAAGAG CTGGAGTGGC AGCAGGTTGT GGATATCCTG 
TGGAGTGACC CTAAACATCA GAATGGCTGC AGTCCCAATT CTTTTCGCGG TGGAGGCTGT 
TACTTCGGGC CAGACGTGAC ACAGACACTG CTGCAGAAAC ACGGCCTCTG TCTGCTCATC 
AGATCACACG AATGCAAACA GGAGGGCTAT GAGCTTTGCC ACAATGGACA GGTTATCACT 
ATATTCTCTG CATCAAACTA CTATGAAGAG GGCAGTAACC GTGGGGCTTA TGTCAAACTG 
GGGCCTGAAC TTGTCCCTCG CTTCTTCCAG TATCAAGTCA GTCGAAGCAC CAGAAAACTC 
ACCCTCCATC AGAGGGTTCG AGTAGCTGAA GGTTCTGCAC TCAGGGCTCT GAAAGAGAAA 
CTTTATGCTC ATCGCTCTGA ACTCATGGCT GCCTTTCAAC AGTATGACAT CAATAACACA 
GGCCGTATCT CCACTAGTGA GTGGGCACAG GTGGTGGAGT CTGTTCTGCG TTTGGATCTG 
CCATGGAGAA CCCTGCGCCC ACGCCTGGCC CGTCTTGGCA CAGATGGCAA TGTGGAATAT 
GAATCCTGTT TCGAGGACAT CAGTCCTGGA GAGCCCATCC CTCCGGTGAC CCCGAACTTA 
GCTGAAACAC TTTATAGATA TAGAACTGAC CTGGAGATCA TTTTTAACAT CATCGATAAG 
GATCACTCAG GTCAGATCTC CATCGAGGAG TTTCGTCAGA CCTGGAAGCT CTTCAGCTCT 
CATTTAGGGG TGGCTGTGAA TGACCAGACC ATAGACGACA TGGCTCGAAG CATAGACTTT 
AACAAAGACG GCAGCATTGA TTTTACTGAG TTCCTGGAGG CCTTCAGGGT GGTGCATAAA 
CTGGATGTCA AAGAGCAACA GCAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304389.1
CDS	1268..3457
Translation	MGCGTSIAIENQTRPSETDGSIKQSNPALTIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQINGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTPRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDINNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	XM_009306114.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306114.2

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGATGGC 
TCCATCAAGC AGTCCAACCC AGCTCTGACC ATAAAAGCTG CCATACTGAT CCAGCGTTGG 
TACAGACGCT ATGTTGCTCA CCTGGAGATG CGAAGACGCT ACACGTGGAA CATCTTTCAG 
TCCATTGAGT ATGCAGGAGA ACAGGACCAG CTACAGCTCT CAAGTTTCTT CACTTTCATG 
TTGGACAACT TCACTCAGAT TAATGGGAAT GGACCAGACT TGATCTCCCA CCTGCTGGAC 
TCTGTGGTTG ATCCTCTGAC GGAGGAGGTC AGGCAGCTGA AGTACGAGCA GATTATGGTG 
CCGGAGTCGT ACAGCGGCCC TCGTCTCTCC TTCCCGCTGA GCGTGACTGA TACCAATGCC 
CTGCTCAGTG CCTTTAAAGA GGAGCAGACT CTTCATGGGA AGTATGTGCT GCAGTTACTG 
TATGAAACCA AGAAGTTCCT GAAACAAATG CCAAACGTCA TTTATCTGTC AACGTCCTAT 
GCCAAAGAAA TAACGATCTG TGGCGATCTG CATGGAAGAC TGGATGACTT GCTGCTTATA 
TTTTACAAGA ACGGTCTGCC CTCTGCGGAA AACCCCTACA TATTCAATGG AGACTTTGTG 
GATCGAGGAA AGAAATCTAC AGAGATTATC ATCATTCTCT TTGCCTTCGT TTTGCTCTAC 
CCCGACCACA TGCATCTGAA CAGGGGAAAC CATGAGGACC ACATCATGAA CCTCAGATAT 
GGATTCACCA GAGAAGTCAT GCAGAAGTAC AAGACTCATG GCAGGGAAAT CCTGCAGCTC 
CTTCAGGATG TGTTCAGTCT TCTTCCTGTT GCAACTGTAA TAGACAACAA AGTGCTTATT 
GTTCATGGTG GCATATCGGA CAAAACTGAT CTGGACTTTC TGAGCACTGT TGAAAGGCAT 
AAAGTGAAGT CAGCTCTGCG TTTGCCGAAG CGCAGCACTG ATCATCTGAC GGTGCGCTCG 
TGCAGTCGCA GCAGCAGCAG AAACAGGGGC AGAAACAGCA GATCCTCAAG CCGACACTCG 
TCCCGCAGCA GAAGGAAGAA GCTCAGCAAG CAGGGCAGCC GCTCCTCCTC TTCCTCATCC 
TCCTCATCCT CCTCTTCCTC CATGTGTTCG CCACGGGTCT CAGCCAAAAA CACTCCACGC 
CGCCCTCCAC CGAGCCCCAC AGACATGCTC CAAGTGCCCT TTCTGGACTC ATTCGTGTCC 
TTGGAGCCAC CAGAGCTGCC CAGAGAAGAG CTGGAGTGGC AGCAGGTTGT GGATATCCTG 
TGGAGTGACC CTAAACATCA GAATGGCTGC AGTCCCAATT CTTTTCGCGG TGGAGGCTGT 
TACTTCGGGC CAGACGTGAC ACAGACACTG CTGCAGAAAC ACGGCCTCTG TCTGCTCATC 
AGATCACACG AATGCAAACA GGAGGGCTAT GAGCTTTGCC ACAATGGACA GGTTATCACT 
ATATTCTCTG CATCAAACTA CTATGAAGAG GGCAGTAACC GTGGGGCTTA TGTCAAACTG 
GGGCCTGAAC TTGTCCCTCG CTTCTTCCAG TATCAAGTCA GTCGAAGCAC CAGAAAACTC 
ACCCTCCATC AGAGGGTTCG AGTAGCTGAA GGTTCTGCAC TCAGGGCTCT GAAAGAGAAA 
CTTTATGCTC ATCGCTCTGA ACTCATGGCT GCCTTTCAAC AGTATGACAT CAATAACACA 
GGCCGTATCT CCACTAGTGA GTGGGCACAG GTGGTGGAGT CTGTTCTGCG TTTGGATCTG 
CCATGGAGAA CCCTGCGCCC ACGCCTGGCC CGTCTTGGCA CAGATGGCAA TGTGGAATAT 
GAATCCTGTT TCGAGGACAT CAGTCCTGGA GAGCCCATCC CTCCGGTGAC CCCGAACTTA 
GCTGAAACAC TTTATAGATA TAGAACTGAC CTGGAGATCA TTTTTAACAT CATCGATAAG 
GATCACTCAG GTCAGATCTC CATCGAGGAG TTTCGTCAGA CCTGGAAGCT CTTCAGCTCT 
CATTTAGGGG TGGCTGTGAA TGACCAGACC ATAGACGACA TGGCTCGAAG CATAGACTTT 
AACAAAGACG GCAGCATTGA TTTTACTGAG TTCCTGGAGG CCTTCAGGGT GGTGCATAAA 
CTGGATGTCA AAGAGCAACA GCAGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_021479632.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335307.1 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa17192
Clone ID Related Accession (Same CDS sequence)	XM_021479632.1 , XM_017358234.1 , NM_001114730.2
Accession Version	XM_021479632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335307.1
CDS	443..2599
Translation	MGCGTSIAIENQTRPSETAIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQINGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTPRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDINNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	XM_021479632.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479632.1

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPEF1 ( XM_017358234.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213723.1 Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X5, mRNA.

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CloneID	ODa17192
Clone ID Related Accession (Same CDS sequence)	XM_021479632.1 , XM_017358234.1 , NM_001114730.2
Accession Version	XM_017358234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein phosphatase with EF-hands 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213723.1
CDS	1246..3402
Translation	MGCGTSIAIENQTRPSETAIKAAILIQRWYRRYVAHLEMRRRYTWNIFQSIEYAGEQDQLQLSSFFTFMLDNFTQINGNGPDLISHLLDSVVDPLTEEVRQLKYEQIMVPESYSGPRLSFPLSVTDTNALLSAFKEEQTLHGKYVLQLLYETKKFLKQMPNVIYLSTSYAKEITICGDLHGRLDDLLLIFYKNGLPSAENPYIFNGDFVDRGKKSTEIIIILFAFVLLYPDHMHLNRGNHEDHIMNLRYGFTREVMQKYKTHGREILQLLQDVFSLLPVATVIDNKVLIVHGGISDKTDLDFLSTVERHKVKSALRLPKRSTDHLTVRSCSRSSSRNRGRNSRSSSRHSSRSRRKKLSKQGSRSSSSSSSSSSSSSMCSPRVSAKNTPRRPPPSPTDMLQVPFLDSFVSLEPPELPREELEWQQVVDILWSDPKHQNGCSPNSFRGGGCYFGPDVTQTLLQKHGLCLLIRSHECKQEGYELCHNGQVITIFSASNYYEEGSNRGAYVKLGPELVPRFFQYQVSRSTRKLTLHQRVRVAEGSALRALKEKLYAHRSELMAAFQQYDINNTGRISTSEWAQVVESVLRLDLPWRTLRPRLARLGTDGNVEYESCFEDISPGEPIPPVTPNLAETLYRYRTDLEIIFNIIDKDHSGQISIEEFRQTWKLFSSHLGVAVNDQTIDDMARSIDFNKDGSIDFTEFLEAFRVVHKLDVKEQQQG

Target ORF information:

RefSeq Version	XM_017358234.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein phosphatase, EF-hand calcium binding domain 1 (ppef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358234.1

ORF Insert Sequence:

ATGGGATGCG GCACATCCAT TGCCATTGAG AACCAAACAC GACCCTCAGA AACAGCCATA 
AAAGCTGCCA TACTGATCCA GCGTTGGTAC AGACGCTATG TTGCTCACCT GGAGATGCGA 
AGACGCTACA CGTGGAACAT CTTTCAGTCC ATTGAGTATG CAGGAGAACA GGACCAGCTA 
CAGCTCTCAA GTTTCTTCAC TTTCATGTTG GACAACTTCA CTCAGATTAA TGGGAATGGA 
CCAGACTTGA TCTCCCACCT GCTGGACTCT GTGGTTGATC CTCTGACGGA GGAGGTCAGG 
CAGCTGAAGT ACGAGCAGAT TATGGTGCCG GAGTCGTACA GCGGCCCTCG TCTCTCCTTC 
CCGCTGAGCG TGACTGATAC CAATGCCCTG CTCAGTGCCT TTAAAGAGGA GCAGACTCTT 
CATGGGAAGT ATGTGCTGCA GTTACTGTAT GAAACCAAGA AGTTCCTGAA ACAAATGCCA 
AACGTCATTT ATCTGTCAAC GTCCTATGCC AAAGAAATAA CGATCTGTGG CGATCTGCAT 
GGAAGACTGG ATGACTTGCT GCTTATATTT TACAAGAACG GTCTGCCCTC TGCGGAAAAC 
CCCTACATAT TCAATGGAGA CTTTGTGGAT CGAGGAAAGA AATCTACAGA GATTATCATC 
ATTCTCTTTG CCTTCGTTTT GCTCTACCCC GACCACATGC ATCTGAACAG GGGAAACCAT 
GAGGACCACA TCATGAACCT CAGATATGGA TTCACCAGAG AAGTCATGCA GAAGTACAAG 
ACTCATGGCA GGGAAATCCT GCAGCTCCTT CAGGATGTGT TCAGTCTTCT TCCTGTTGCA 
ACTGTAATAG ACAACAAAGT GCTTATTGTT CATGGTGGCA TATCGGACAA AACTGATCTG 
GACTTTCTGA GCACTGTTGA AAGGCATAAA GTGAAGTCAG CTCTGCGTTT GCCGAAGCGC 
AGCACTGATC ATCTGACGGT GCGCTCGTGC AGTCGCAGCA GCAGCAGAAA CAGGGGCAGA 
AACAGCAGAT CCTCAAGCCG ACACTCGTCC CGCAGCAGAA GGAAGAAGCT CAGCAAGCAG 
GGCAGCCGCT CCTCCTCTTC CTCATCCTCC TCATCCTCCT CTTCCTCCAT GTGTTCGCCA 
CGGGTCTCAG CCAAAAACAC TCCACGCCGC CCTCCACCGA GCCCCACAGA CATGCTCCAA 
GTGCCCTTTC TGGACTCATT CGTGTCCTTG GAGCCACCAG AGCTGCCCAG AGAAGAGCTG 
GAGTGGCAGC AGGTTGTGGA TATCCTGTGG AGTGACCCTA AACATCAGAA TGGCTGCAGT 
CCCAATTCTT TTCGCGGTGG AGGCTGTTAC TTCGGGCCAG ACGTGACACA GACACTGCTG 
CAGAAACACG GCCTCTGTCT GCTCATCAGA TCACACGAAT GCAAACAGGA GGGCTATGAG 
CTTTGCCACA ATGGACAGGT TATCACTATA TTCTCTGCAT CAAACTACTA TGAAGAGGGC 
AGTAACCGTG GGGCTTATGT CAAACTGGGG CCTGAACTTG TCCCTCGCTT CTTCCAGTAT 
CAAGTCAGTC GAAGCACCAG AAAACTCACC CTCCATCAGA GGGTTCGAGT AGCTGAAGGT 
TCTGCACTCA GGGCTCTGAA AGAGAAACTT TATGCTCATC GCTCTGAACT CATGGCTGCC 
TTTCAACAGT ATGACATCAA TAACACAGGC CGTATCTCCA CTAGTGAGTG GGCACAGGTG 
GTGGAGTCTG TTCTGCGTTT GGATCTGCCA TGGAGAACCC TGCGCCCACG CCTGGCCCGT 
CTTGGCACAG ATGGCAATGT GGAATATGAA TCCTGTTTCG AGGACATCAG TCCTGGAGAG 
CCCATCCCTC CGGTGACCCC GAACTTAGCT GAAACACTTT ATAGATATAG AACTGACCTG 
GAGATCATTT TTAACATCAT CGATAAGGAT CACTCAGGTC AGATCTCCAT CGAGGAGTTT 
CGTCAGACCT GGAAGCTCTT CAGCTCTCAT TTAGGGGTGG CTGTGAATGA CCAGACCATA 
GACGACATGG CTCGAAGCAT AGACTTTAAC AAAGACGGCA GCATTGATTT TACTGAGTTC 
CTGGAGGCCT TCAGGGTGGT GCATAAACTG GATGTCAAAG AGCAACAGCA GGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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PPEF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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