SMAD4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMAD4A
Entrez Gene ID	559111
Full Name	SMAD family member 4a
Synonyms	madh4,smad4,zmp:0000000768
General protein information	Preferred Names SMAD family member 4a Names mothers against decapentaplegic homolog 4 MAD (mothers against decapentaplegic, Drosophila) homolog 4 MAD homolog 4 SMA- and MAD-related protein 4 SMAD family member 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122700.1	NP_001116172.1	mothers against decapentaplegic homolog 4
XM_017357976.2	XP_017213465.1	mothers against decapentaplegic homolog 4 isoform X1
XM_017357977.2	XP_017213466.1	mothers against decapentaplegic homolog 4 isoform X2
XM_017357978.1	XP_017213467.1	mothers against decapentaplegic homolog 4 isoform X3
XM_017357978.2	XP_017213467.1	mothers against decapentaplegic homolog 4 isoform X3
XM_017357976.1	XP_017213465.1	mothers against decapentaplegic homolog 4 isoform X1
XM_017357977.1	XP_017213466.1	mothers against decapentaplegic homolog 4 isoform X2

Pathways from BioSystems

Homologies

	SMAD4	XP_001096658.1
Mus musculus (house mouse)	Smad4	NP_032566.2
Drosophila melanogaster (fruit fly)	Med	NP_524610.1
Xenopus tropicalis (tropical clawed frog)	smad4.1	XP_002934485.1
Bos taurus (cattle)	SMAD4	NP_001069677.1
Danio rerio (zebrafish)	zmp:0000000768	NP_001116172.1
Homo sapiens (human)	SMAD4	NP_005350.1
Canis lupus familiaris (dog)	SMAD4	XP_005615451.1
Rattus norvegicus (Norway rat)	Smad4	NP_062148.1
Pan troglodytes (chimpanzee)	SMAD4	XP_001155601.1

Gene Ontology
Bibliography

Related articles in PubMed

Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification.
Hozumi S, Aoki S, Kikuchi Y
Development (Cambridge, England)144(21)4015-4025(2017 11)

Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-? signaling.
Liu JX, Xu QH, Li S, Yu X, Liu W, Ouyang G, Zhang T, Chen LL
Biochimica et biophysica acta1860(10)1103-1116(2017 10)

Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo.
Bian SS, Zheng XL, Sun HQ, Chen JH, Lu YL, Liu YQ, Tao DC, Ma YX
The Journal of biological chemistry292(34)14165-14175(2017 08)

USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.
Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, van Dinther M, Sixma TK, Huang H, Zhang L, Ten Dijke P
The EMBO journal36(11)1623-1639(2017 06)

Transforming Growth Factor ? Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells.
Monteiro R, Pinheiro P, Joseph N, Peterkin T, Koth J, Repapi E, Bonkhofer F, Kirmizitas A, Patient R
Developmental cell38(4)358-70(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a notable increase in apoptosis was apparent in the smad4 knockdown embryos, while no obvious reduction in cell proliferation was observed. Collectively, these data suggest that smad4 plays an important role in heart and skeletal muscle development.
Title: Smad4 is required for the development of cardiac and skeletal muscle in zebrafish.

Dkk3r regulates p38a phosphorylation to maintain Smad4 stability, in turn enabling the Smad2.Smad3a.Smad4 complex to form and activate the myf5 promoter.
Title: dickkopf-3-related gene regulates the expression of zebrafish myf5 gene through phosphorylated p38a-dependent Smad4 activity.

data presented show that Zili suppresses TGF-beta signaling by physically associating with Smad4 and preventing the formation of Smad2/3/4 and Smad1/5/9/4 complexes
Title: Zili inhibits transforming growth factor-beta signaling by interacting with Smad4.

The following SMAD4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMAD4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35853	NM_001122700.1 Latest version!	Danio rerio SMAD family member 4a (smad4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54027	XM_017357976.2 Latest version!	Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54028	XM_017357977.2 Latest version!	Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54029	XM_017357978.2 Latest version!	Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa54029	XM_017357978.1	Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54027	XM_017357976.1	Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa54028	XM_017357977.1	Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMAD4A ( NM_001122700.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001116172.1 Danio rerio SMAD family member 4a (smad4a), mRNA.

Price & Availability↑

CloneID	ODa35853
Clone ID Related Accession (Same CDS sequence)	NM_001122700.1
Accession Version	NM_001122700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU489481.1. On Mar 19, 2008 this sequence version replaced XR_030070.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU489481.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CTCCTGAATA CTGGTGCTCC 
ATTGCCTACT TCGAGATGGA CGTTCAGGTG GGCGAGACCT TCAAAGTGCC CTCGTCCTGC 
CCCATCGTGA CGGTGGATGG GTACGTGGAT CCGTCTGGAG GAGACAGATT CTGCCTTGGC 
CAGCTCAGCA ACGTCCACAG AACAGAGGCC ATCGAGAGAG CGAGACTGCA CATCGGGAAG 
GGCGTCCAGC TGGAGTGTAA AGGTGAAGGA GACGTGTGGG TTCGCTGTCT GAGCGATCAC 
GCTGTCTTCG TCCAGAGCTA CTACCTGGAC CGTGAGGCGG GACGCGCACC CGGGGACGCC 
GTGCACAAGA TTTACCCCAG CGCATACATC AAGGTGTTTG ACTTGCGTCA GTGCCACAGG 
CAGATGCAGC AGCAGGCAGC GACGGCTCAG GCAGCAGCAG CCGCTCAGGC AGCAGCAGTG 
GCTGGAAACA TCCCCGGACC CGGATCTGTC GGAGGAATCG CTCCAGCTAT CAGTTTGTCT 
GCAGCCGCTG GTATCGGTGT GGATGACCTG CGCCGGCTCT GTATCCTGCG CATGAGCTTC 
GTGAAGGGCT GGGGTCCCGA TTACCCCCGG CAGAGCATTA AAGAGACGCC CTGCTGGATC 
GAGATCCATA TGCACCGTGC GCTGCAGCTG CTGGACGAGG TTCTGCACAC CATGCCCATC 
GCAGACCCCA CACCGTTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116172.1
CDS	411..2054
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTTQPGSVLSGSHSSDSLLQIASGTGQGSQQNGFPPGQTSTYHHNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHMHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	NM_001122700.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122700.1

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CTCCTGAATA CTGGTGCTCC 
ATTGCCTACT TCGAGATGGA CGTTCAGGTG GGCGAGACCT TCAAAGTGCC CTCGTCCTGC 
CCCATCGTGA CGGTGGATGG GTACGTGGAT CCGTCTGGAG GAGACAGATT CTGCCTTGGC 
CAGCTCAGCA ACGTCCACAG AACAGAGGCC ATCGAGAGAG CGAGACTGCA CATCGGGAAG 
GGCGTCCAGC TGGAGTGTAA AGGTGAAGGA GACGTGTGGG TTCGCTGTCT GAGCGATCAC 
GCTGTCTTCG TCCAGAGCTA CTACCTGGAC CGTGAGGCGG GACGCGCACC CGGGGACGCC 
GTGCACAAGA TTTACCCCAG CGCATACATC AAGGTGTTTG ACTTGCGTCA GTGCCACAGG 
CAGATGCAGC AGCAGGCAGC GACGGCTCAG GCAGCAGCAG CCGCTCAGGC AGCAGCAGTG 
GCTGGAAACA TCCCCGGACC CGGATCTGTC GGAGGAATCG CTCCAGCTAT CAGTTTGTCT 
GCAGCCGCTG GTATCGGTGT GGATGACCTG CGCCGGCTCT GTATCCTGCG CATGAGCTTC 
GTGAAGGGCT GGGGTCCCGA TTACCCCCGG CAGAGCATTA AAGAGACGCC CTGCTGGATC 
GAGATCCATA TGCACCGTGC GCTGCAGCTG CTGGACGAGG TTCTGCACAC CATGCCCATC 
GCAGACCCCA CACCGTTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357976.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213465.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54027
Clone ID Related Accession (Same CDS sequence)	XM_017357976.2 , XM_017357976.1
Accession Version	XM_017357976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CAGCTCCTGA ATACTGGTGC 
TCCATTGCCT ACTTCGAGAT GGACGTTCAG GTGGGCGAGA CCTTCAAAGT GCCCTCGTCC 
TGCCCCATCG TGACGGTGGA TGGGTACGTG GACCCGTCTG GAGGAGACAG ATTCTGCCTC 
GGCCAGCTCA GCAACGTCCA CAGAACAGAG GCCATCGAGA GAGCGAGACT GCACATCGGG 
AAGGGCGTCC AGCTGGAGTG TAAAGGTGAA GGAGACGTGT GGGTTCGCTG TCTGAGCGAT 
CACGCGGTCT TCGTCCAGAG CTACTACCTG GACCGTGAGG CGGGACGCGC ACCCGGGGAC 
GCCGTGCACA AGATTTACCC CAGCGCATAC ATCAAGGTGT TTGACTTGCG TCAGTGCCAC 
CGGCAGATGC AGCAGCAGGC AGCGACGGCT CAGGCAGCGG CAGCCGCTCA GGCAGCAGCA 
GTGGCCGGAA ACATCCCCGG ACCCGGATCT GTCGGAGGAA TCGCTCCAGC TATCAGTTTG 
TCTGCGGCCG CTGGTATCGG TGTGGATGAC CTGCGCCGGC TCTGTATCCT GCGCATGAGC 
TTCGTGAAGG GCTGGGGTCC CGATTACCCC CGGCAGAGCA TTAAAGAGAC GCCCTGCTGG 
ATCGAGATCC ATCTGCACCG TGCGCTGCAG CTGCTGGACG AGGTTCTGCA CACCATGCCC 
ATCGCAGACC CCACACCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213465.1
CDS	391..2037
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTTQPGSVLSGSHSSDSLLQIASGTGQGSQQNGFPPGQTSTYHHNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357976.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357976.2

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CAGCTCCTGA ATACTGGTGC 
TCCATTGCCT ACTTCGAGAT GGACGTTCAG GTGGGCGAGA CCTTCAAAGT GCCCTCGTCC 
TGCCCCATCG TGACGGTGGA TGGGTACGTG GACCCGTCTG GAGGAGACAG ATTCTGCCTC 
GGCCAGCTCA GCAACGTCCA CAGAACAGAG GCCATCGAGA GAGCGAGACT GCACATCGGG 
AAGGGCGTCC AGCTGGAGTG TAAAGGTGAA GGAGACGTGT GGGTTCGCTG TCTGAGCGAT 
CACGCGGTCT TCGTCCAGAG CTACTACCTG GACCGTGAGG CGGGACGCGC ACCCGGGGAC 
GCCGTGCACA AGATTTACCC CAGCGCATAC ATCAAGGTGT TTGACTTGCG TCAGTGCCAC 
CGGCAGATGC AGCAGCAGGC AGCGACGGCT CAGGCAGCGG CAGCCGCTCA GGCAGCAGCA 
GTGGCCGGAA ACATCCCCGG ACCCGGATCT GTCGGAGGAA TCGCTCCAGC TATCAGTTTG 
TCTGCGGCCG CTGGTATCGG TGTGGATGAC CTGCGCCGGC TCTGTATCCT GCGCATGAGC 
TTCGTGAAGG GCTGGGGTCC CGATTACCCC CGGCAGAGCA TTAAAGAGAC GCCCTGCTGG 
ATCGAGATCC ATCTGCACCG TGCGCTGCAG CTGCTGGACG AGGTTCTGCA CACCATGCCC 
ATCGCAGACC CCACACCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357976.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213465.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54027
Clone ID Related Accession (Same CDS sequence)	XM_017357976.2 , XM_017357976.1
Accession Version	XM_017357976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CAGCTCCTGA ATACTGGTGC 
TCCATTGCCT ACTTCGAGAT GGACGTTCAG GTGGGCGAGA CCTTCAAAGT GCCCTCGTCC 
TGCCCCATCG TGACGGTGGA TGGGTACGTG GACCCGTCTG GAGGAGACAG ATTCTGCCTC 
GGCCAGCTCA GCAACGTCCA CAGAACAGAG GCCATCGAGA GAGCGAGACT GCACATCGGG 
AAGGGCGTCC AGCTGGAGTG TAAAGGTGAA GGAGACGTGT GGGTTCGCTG TCTGAGCGAT 
CACGCGGTCT TCGTCCAGAG CTACTACCTG GACCGTGAGG CGGGACGCGC ACCCGGGGAC 
GCCGTGCACA AGATTTACCC CAGCGCATAC ATCAAGGTGT TTGACTTGCG TCAGTGCCAC 
CGGCAGATGC AGCAGCAGGC AGCGACGGCT CAGGCAGCGG CAGCCGCTCA GGCAGCAGCA 
GTGGCCGGAA ACATCCCCGG ACCCGGATCT GTCGGAGGAA TCGCTCCAGC TATCAGTTTG 
TCTGCGGCCG CTGGTATCGG TGTGGATGAC CTGCGCCGGC TCTGTATCCT GCGCATGAGC 
TTCGTGAAGG GCTGGGGTCC CGATTACCCC CGGCAGAGCA TTAAAGAGAC GCCCTGCTGG 
ATCGAGATCC ATCTGCACCG TGCGCTGCAG CTGCTGGACG AGGTTCTGCA CACCATGCCC 
ATCGCAGACC CCACACCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213465.1
CDS	393..2039
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTTQPGSVLSGSHSSDSLLQIASGTGQGSQQNGFPPGQTSTYHHNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357976.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357976.1

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAACTCAG 
CCCGGTAGTG TGCTCTCAGG AAGCCACAGC AGTGATTCTC TGCTGCAGAT TGCGTCAGGC 
ACAGGGCAGG GCTCACAGCA GAATGGCTTT CCTCCAGGCC AGACCTCCAC ATACCACCAC 
AATCCCACCT CCTCCTGGAG CAGGAACAGT AACTTCACCC CCAGCGTGCC TCATCATCAG 
AACGGGCATC TGCAGCATCA CCCGCCCATG GCTCATCCGG CACACTACTG GCCTGTTCAC 
AATGAAATCG CATTTCAGCC ACCGATATCC AACCACCCTG CAGCTCCTGA ATACTGGTGC 
TCCATTGCCT ACTTCGAGAT GGACGTTCAG GTGGGCGAGA CCTTCAAAGT GCCCTCGTCC 
TGCCCCATCG TGACGGTGGA TGGGTACGTG GACCCGTCTG GAGGAGACAG ATTCTGCCTC 
GGCCAGCTCA GCAACGTCCA CAGAACAGAG GCCATCGAGA GAGCGAGACT GCACATCGGG 
AAGGGCGTCC AGCTGGAGTG TAAAGGTGAA GGAGACGTGT GGGTTCGCTG TCTGAGCGAT 
CACGCGGTCT TCGTCCAGAG CTACTACCTG GACCGTGAGG CGGGACGCGC ACCCGGGGAC 
GCCGTGCACA AGATTTACCC CAGCGCATAC ATCAAGGTGT TTGACTTGCG TCAGTGCCAC 
CGGCAGATGC AGCAGCAGGC AGCGACGGCT CAGGCAGCGG CAGCCGCTCA GGCAGCAGCA 
GTGGCCGGAA ACATCCCCGG ACCCGGATCT GTCGGAGGAA TCGCTCCAGC TATCAGTTTG 
TCTGCGGCCG CTGGTATCGG TGTGGATGAC CTGCGCCGGC TCTGTATCCT GCGCATGAGC 
TTCGTGAAGG GCTGGGGTCC CGATTACCCC CGGCAGAGCA TTAAAGAGAC GCCCTGCTGG 
ATCGAGATCC ATCTGCACCG TGCGCTGCAG CTGCTGGACG AGGTTCTGCA CACCATGCCC 
ATCGCAGACC CCACACCGTT AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357977.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213466.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54028
Clone ID Related Accession (Same CDS sequence)	XM_017357977.2 , XM_017357977.1
Accession Version	XM_017357977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCAGCTC CTGAATACTG GTGCTCCATT 
GCCTACTTCG AGATGGACGT TCAGGTGGGC GAGACCTTCA AAGTGCCCTC GTCCTGCCCC 
ATCGTGACGG TGGATGGGTA CGTGGACCCG TCTGGAGGAG ACAGATTCTG CCTCGGCCAG 
CTCAGCAACG TCCACAGAAC AGAGGCCATC GAGAGAGCGA GACTGCACAT CGGGAAGGGC 
GTCCAGCTGG AGTGTAAAGG TGAAGGAGAC GTGTGGGTTC GCTGTCTGAG CGATCACGCG 
GTCTTCGTCC AGAGCTACTA CCTGGACCGT GAGGCGGGAC GCGCACCCGG GGACGCCGTG 
CACAAGATTT ACCCCAGCGC ATACATCAAG GTGTTTGACT TGCGTCAGTG CCACCGGCAG 
ATGCAGCAGC AGGCAGCGAC GGCTCAGGCA GCGGCAGCCG CTCAGGCAGC AGCAGTGGCC 
GGAAACATCC CCGGACCCGG ATCTGTCGGA GGAATCGCTC CAGCTATCAG TTTGTCTGCG 
GCCGCTGGTA TCGGTGTGGA TGACCTGCGC CGGCTCTGTA TCCTGCGCAT GAGCTTCGTG 
AAGGGCTGGG GTCCCGATTA CCCCCGGCAG AGCATTAAAG AGACGCCCTG CTGGATCGAG 
ATCCATCTGC ACCGTGCGCT GCAGCTGCTG GACGAGGTTC TGCACACCAT GCCCATCGCA 
GACCCCACAC CGTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213466.1
CDS	391..1911
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357977.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357977.2

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCAGCTC CTGAATACTG GTGCTCCATT 
GCCTACTTCG AGATGGACGT TCAGGTGGGC GAGACCTTCA AAGTGCCCTC GTCCTGCCCC 
ATCGTGACGG TGGATGGGTA CGTGGACCCG TCTGGAGGAG ACAGATTCTG CCTCGGCCAG 
CTCAGCAACG TCCACAGAAC AGAGGCCATC GAGAGAGCGA GACTGCACAT CGGGAAGGGC 
GTCCAGCTGG AGTGTAAAGG TGAAGGAGAC GTGTGGGTTC GCTGTCTGAG CGATCACGCG 
GTCTTCGTCC AGAGCTACTA CCTGGACCGT GAGGCGGGAC GCGCACCCGG GGACGCCGTG 
CACAAGATTT ACCCCAGCGC ATACATCAAG GTGTTTGACT TGCGTCAGTG CCACCGGCAG 
ATGCAGCAGC AGGCAGCGAC GGCTCAGGCA GCGGCAGCCG CTCAGGCAGC AGCAGTGGCC 
GGAAACATCC CCGGACCCGG ATCTGTCGGA GGAATCGCTC CAGCTATCAG TTTGTCTGCG 
GCCGCTGGTA TCGGTGTGGA TGACCTGCGC CGGCTCTGTA TCCTGCGCAT GAGCTTCGTG 
AAGGGCTGGG GTCCCGATTA CCCCCGGCAG AGCATTAAAG AGACGCCCTG CTGGATCGAG 
ATCCATCTGC ACCGTGCGCT GCAGCTGCTG GACGAGGTTC TGCACACCAT GCCCATCGCA 
GACCCCACAC CGTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357977.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213466.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54028
Clone ID Related Accession (Same CDS sequence)	XM_017357977.2 , XM_017357977.1
Accession Version	XM_017357977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCAGCTC CTGAATACTG GTGCTCCATT 
GCCTACTTCG AGATGGACGT TCAGGTGGGC GAGACCTTCA AAGTGCCCTC GTCCTGCCCC 
ATCGTGACGG TGGATGGGTA CGTGGACCCG TCTGGAGGAG ACAGATTCTG CCTCGGCCAG 
CTCAGCAACG TCCACAGAAC AGAGGCCATC GAGAGAGCGA GACTGCACAT CGGGAAGGGC 
GTCCAGCTGG AGTGTAAAGG TGAAGGAGAC GTGTGGGTTC GCTGTCTGAG CGATCACGCG 
GTCTTCGTCC AGAGCTACTA CCTGGACCGT GAGGCGGGAC GCGCACCCGG GGACGCCGTG 
CACAAGATTT ACCCCAGCGC ATACATCAAG GTGTTTGACT TGCGTCAGTG CCACCGGCAG 
ATGCAGCAGC AGGCAGCGAC GGCTCAGGCA GCGGCAGCCG CTCAGGCAGC AGCAGTGGCC 
GGAAACATCC CCGGACCCGG ATCTGTCGGA GGAATCGCTC CAGCTATCAG TTTGTCTGCG 
GCCGCTGGTA TCGGTGTGGA TGACCTGCGC CGGCTCTGTA TCCTGCGCAT GAGCTTCGTG 
AAGGGCTGGG GTCCCGATTA CCCCCGGCAG AGCATTAAAG AGACGCCCTG CTGGATCGAG 
ATCCATCTGC ACCGTGCGCT GCAGCTGCTG GACGAGGTTC TGCACACCAT GCCCATCGCA 
GACCCCACAC CGTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213466.1
CDS	393..1913
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357977.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357977.1

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCAGCTC CTGAATACTG GTGCTCCATT 
GCCTACTTCG AGATGGACGT TCAGGTGGGC GAGACCTTCA AAGTGCCCTC GTCCTGCCCC 
ATCGTGACGG TGGATGGGTA CGTGGACCCG TCTGGAGGAG ACAGATTCTG CCTCGGCCAG 
CTCAGCAACG TCCACAGAAC AGAGGCCATC GAGAGAGCGA GACTGCACAT CGGGAAGGGC 
GTCCAGCTGG AGTGTAAAGG TGAAGGAGAC GTGTGGGTTC GCTGTCTGAG CGATCACGCG 
GTCTTCGTCC AGAGCTACTA CCTGGACCGT GAGGCGGGAC GCGCACCCGG GGACGCCGTG 
CACAAGATTT ACCCCAGCGC ATACATCAAG GTGTTTGACT TGCGTCAGTG CCACCGGCAG 
ATGCAGCAGC AGGCAGCGAC GGCTCAGGCA GCGGCAGCCG CTCAGGCAGC AGCAGTGGCC 
GGAAACATCC CCGGACCCGG ATCTGTCGGA GGAATCGCTC CAGCTATCAG TTTGTCTGCG 
GCCGCTGGTA TCGGTGTGGA TGACCTGCGC CGGCTCTGTA TCCTGCGCAT GAGCTTCGTG 
AAGGGCTGGG GTCCCGATTA CCCCCGGCAG AGCATTAAAG AGACGCCCTG CTGGATCGAG 
ATCCATCTGC ACCGTGCGCT GCAGCTGCTG GACGAGGTTC TGCACACCAT GCCCATCGCA 
GACCCCACAC CGTTAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357978.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213467.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa54029
Clone ID Related Accession (Same CDS sequence)	XM_017357978.1 , XM_017357978.2
Accession Version	XM_017357978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCTCCTG AATACTGGTG CTCCATTGCC 
TACTTCGAGA TGGACGTTCA GGTGGGCGAG ACCTTCAAAG TGCCCTCGTC CTGCCCCATC 
GTGACGGTGG ATGGGTACGT GGACCCGTCT GGAGGAGACA GATTCTGCCT CGGCCAGCTC 
AGCAACGTCC ACAGAACAGA GGCCATCGAG AGAGCGAGAC TGCACATCGG GAAGGGCGTC 
CAGCTGGAGT GTAAAGGTGA AGGAGACGTG TGGGTTCGCT GTCTGAGCGA TCACGCGGTC 
TTCGTCCAGA GCTACTACCT GGACCGTGAG GCGGGACGCG CACCCGGGGA CGCCGTGCAC 
AAGATTTACC CCAGCGCATA CATCAAGGTG TTTGACTTGC GTCAGTGCCA CCGGCAGATG 
CAGCAGCAGG CAGCGACGGC TCAGGCAGCG GCAGCCGCTC AGGCAGCAGC AGTGGCCGGA 
AACATCCCCG GACCCGGATC TGTCGGAGGA ATCGCTCCAG CTATCAGTTT GTCTGCGGCC 
GCTGGTATCG GTGTGGATGA CCTGCGCCGG CTCTGTATCC TGCGCATGAG CTTCGTGAAG 
GGCTGGGGTC CCGATTACCC CCGGCAGAGC ATTAAAGAGA CGCCCTGCTG GATCGAGATC 
CATCTGCACC GTGCGCTGCA GCTGCTGGAC GAGGTTCTGC ACACCATGCC CATCGCAGAC 
CCCACACCGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213467.1
CDS	392..1909
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357978.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357978.1

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCTCCTG AATACTGGTG CTCCATTGCC 
TACTTCGAGA TGGACGTTCA GGTGGGCGAG ACCTTCAAAG TGCCCTCGTC CTGCCCCATC 
GTGACGGTGG ATGGGTACGT GGACCCGTCT GGAGGAGACA GATTCTGCCT CGGCCAGCTC 
AGCAACGTCC ACAGAACAGA GGCCATCGAG AGAGCGAGAC TGCACATCGG GAAGGGCGTC 
CAGCTGGAGT GTAAAGGTGA AGGAGACGTG TGGGTTCGCT GTCTGAGCGA TCACGCGGTC 
TTCGTCCAGA GCTACTACCT GGACCGTGAG GCGGGACGCG CACCCGGGGA CGCCGTGCAC 
AAGATTTACC CCAGCGCATA CATCAAGGTG TTTGACTTGC GTCAGTGCCA CCGGCAGATG 
CAGCAGCAGG CAGCGACGGC TCAGGCAGCG GCAGCCGCTC AGGCAGCAGC AGTGGCCGGA 
AACATCCCCG GACCCGGATC TGTCGGAGGA ATCGCTCCAG CTATCAGTTT GTCTGCGGCC 
GCTGGTATCG GTGTGGATGA CCTGCGCCGG CTCTGTATCC TGCGCATGAG CTTCGTGAAG 
GGCTGGGGTC CCGATTACCC CCGGCAGAGC ATTAAAGAGA CGCCCTGCTG GATCGAGATC 
CATCTGCACC GTGCGCTGCA GCTGCTGGAC GAGGTTCTGC ACACCATGCC CATCGCAGAC 
CCCACACCGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMAD4A ( XM_017357978.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213467.1 Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.

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CloneID	ODa54029
Clone ID Related Accession (Same CDS sequence)	XM_017357978.1 , XM_017357978.2
Accession Version	XM_017357978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mothers against decapentaplegic homolog 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCTCCTG AATACTGGTG CTCCATTGCC 
TACTTCGAGA TGGACGTTCA GGTGGGCGAG ACCTTCAAAG TGCCCTCGTC CTGCCCCATC 
GTGACGGTGG ATGGGTACGT GGACCCGTCT GGAGGAGACA GATTCTGCCT CGGCCAGCTC 
AGCAACGTCC ACAGAACAGA GGCCATCGAG AGAGCGAGAC TGCACATCGG GAAGGGCGTC 
CAGCTGGAGT GTAAAGGTGA AGGAGACGTG TGGGTTCGCT GTCTGAGCGA TCACGCGGTC 
TTCGTCCAGA GCTACTACCT GGACCGTGAG GCGGGACGCG CACCCGGGGA CGCCGTGCAC 
AAGATTTACC CCAGCGCATA CATCAAGGTG TTTGACTTGC GTCAGTGCCA CCGGCAGATG 
CAGCAGCAGG CAGCGACGGC TCAGGCAGCG GCAGCCGCTC AGGCAGCAGC AGTGGCCGGA 
AACATCCCCG GACCCGGATC TGTCGGAGGA ATCGCTCCAG CTATCAGTTT GTCTGCGGCC 
GCTGGTATCG GTGTGGATGA CCTGCGCCGG CTCTGTATCC TGCGCATGAG CTTCGTGAAG 
GGCTGGGGTC CCGATTACCC CCGGCAGAGC ATTAAAGAGA CGCCCTGCTG GATCGAGATC 
CATCTGCACC GTGCGCTGCA GCTGCTGGAC GAGGTTCTGC ACACCATGCC CATCGCAGAC 
CCCACACCGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213467.1
CDS	390..1907
Translation	MSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQFAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLSGSGPSGLMVKDEYDYDGPPSLPSTEGHMQTIQHPPSRAVTQEPFSTPALLPPAEGSSSASSSAFSSIAVGSTNPTSSWSRNSNFTPSVPHHQNGHLQHHPPMAHPAHYWPVHNEIAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPTPLD

Target ORF information:

RefSeq Version	XM_017357978.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SMAD family member 4a (smad4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357978.2

ORF Insert Sequence:

ATGTCCATCA CAAACACTCC CACCAGTAAT GACGCGTGCC TGAGCATCGT GCACAGTCTG 
ATGTGCCACC GGCAGGGCGG CGAGAGCGAG ACCTTCGCCA AACGCGCCAT CGAGAGCCTG 
GTGAAGAAGC TAAAGGAGAA GAAAGATGAG CTGGATTCTC TGATCACCGC TATCACCACT 
AACGGAGCGC ATCCCAGCAA ATGTGTGACC ATACAGAGAA CGCTGGACGG ACGGCTGCAG 
GTGGCGGGCC GGAAAGGATT CCCTCATGTG ATTTATGCAC GGTTATGGCG ATGGCCTGAT 
CTTCATAAGA ACGAGCTGAA GCATGTCAAA TACTGCCAGT TCGCCTTCGA CCTGAAGTGT 
GACAGCGTGT GCGTCAACCC CTACCACTAC GAGAGAGTGG TGTCTCCAGG AATAGATCTG 
TCTGGCCTCA CACTGTCAGG CTCTGGTCCG TCAGGGCTGA TGGTGAAGGA TGAGTATGAT 
TATGATGGGC CACCGTCTCT GCCTAGCACT GAGGGACACA TGCAGACCAT CCAGCACCCG 
CCGTCCCGCG CCGTCACCCA GGAGCCCTTC AGCACGCCCG CCCTGCTCCC GCCAGCAGAA 
GGCAGCAGCT CCGCCTCCAG CTCCGCCTTC TCCAGCATCG CCGTCGGATC CACAAATCCC 
ACCTCCTCCT GGAGCAGGAA CAGTAACTTC ACCCCCAGCG TGCCTCATCA TCAGAACGGG 
CATCTGCAGC ATCACCCGCC CATGGCTCAT CCGGCACACT ACTGGCCTGT TCACAATGAA 
ATCGCATTTC AGCCACCGAT ATCCAACCAC CCTGCTCCTG AATACTGGTG CTCCATTGCC 
TACTTCGAGA TGGACGTTCA GGTGGGCGAG ACCTTCAAAG TGCCCTCGTC CTGCCCCATC 
GTGACGGTGG ATGGGTACGT GGACCCGTCT GGAGGAGACA GATTCTGCCT CGGCCAGCTC 
AGCAACGTCC ACAGAACAGA GGCCATCGAG AGAGCGAGAC TGCACATCGG GAAGGGCGTC 
CAGCTGGAGT GTAAAGGTGA AGGAGACGTG TGGGTTCGCT GTCTGAGCGA TCACGCGGTC 
TTCGTCCAGA GCTACTACCT GGACCGTGAG GCGGGACGCG CACCCGGGGA CGCCGTGCAC 
AAGATTTACC CCAGCGCATA CATCAAGGTG TTTGACTTGC GTCAGTGCCA CCGGCAGATG 
CAGCAGCAGG CAGCGACGGC TCAGGCAGCG GCAGCCGCTC AGGCAGCAGC AGTGGCCGGA 
AACATCCCCG GACCCGGATC TGTCGGAGGA ATCGCTCCAG CTATCAGTTT GTCTGCGGCC 
GCTGGTATCG GTGTGGATGA CCTGCGCCGG CTCTGTATCC TGCGCATGAG CTTCGTGAAG 
GGCTGGGGTC CCGATTACCC CCGGCAGAGC ATTAAAGAGA CGCCCTGCTG GATCGAGATC 
CATCTGCACC GTGCGCTGCA GCTGCTGGAC GAGGTTCTGC ACACCATGCC CATCGCAGAC 
CCCACACCGT TAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nuclear movement regulated by non-Smad Nodal signaling via JNK is associated with Smad signaling during zebrafish endoderm specification.
Development (Cambridge, England)144(21)4015-4025(2017 11)
Hozumi S,Aoki S,Kikuchi Y

Transcriptional factors Eaf1/2 inhibit endoderm and mesoderm formation via suppressing TGF-? signaling.
Biochimica et biophysica acta1860(10)1103-1116(2017 10)
Liu JX,Xu QH,Li S,Yu X,Liu W,Ouyang G,Zhang T,Chen LL

Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo.
The Journal of biological chemistry292(34)14165-14175(2017 08)
Bian SS,Zheng XL,Sun HQ,Chen JH,Lu YL,Liu YQ,Tao DC,Ma YX

USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling.
The EMBO journal36(11)1623-1639(2017 06)
Zhou F,Xie F,Jin K,Zhang Z,Clerici M,Gao R,van Dinther M,Sixma TK,Huang H,Zhang L,Ten Dijke P

Transforming Growth Factor ? Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells.
Developmental cell38(4)358-70(2016 08)
Monteiro R,Pinheiro P,Joseph N,Peterkin T,Koth J,Repapi E,Bonkhofer F,Kirmizitas A,Patient R

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SMAD4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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