USP30 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP30
Entrez Gene ID	559099
Full Name	ubiquitin specific peptidase 30
Synonyms	si:dkey-72n1.2
General protein information	Preferred Names ubiquitin specific peptidase 30 Names ubiquitin carboxyl-terminal hydrolase 30 Deubiquitinating enzyme 30 Ub-specific protease 30 Ubiquitin thioesterase 30 Ubiquitin-specific protease 30 ubiquitin thiolesterase 30 ubiquitin-specific-processing protease 30
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001162484.1	NP_001155956.1	ubiquitin carboxyl-terminal hydrolase 30
XM_005165156.4	XP_005165213.1	ubiquitin carboxyl-terminal hydrolase 30 isoform X1
XM_005165156.3	XP_005165213.1	ubiquitin carboxyl-terminal hydrolase 30 isoform X1

Pathways from BioSystems

Homologies

	USP30	XP_001104096.1
Mus musculus (house mouse)	Usp30	NP_001028374.1
Drosophila melanogaster (fruit fly)	CG3016	NP_572274.1
Xenopus tropicalis (tropical clawed frog)	usp30	NP_001039085.1
Gallus gallus (chicken)	USP30	XP_415187.3
Danio rerio (zebrafish)	usp30	NP_001155956.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000269	XP_310850.4
Caenorhabditis elegans (roundworm)	Y67D2.2	NP_497422.3
Rattus norvegicus (Norway rat)	Usp30	NP_001100623.1
Bos taurus (cattle)	USP30	NP_001178290.1
Danio rerio (zebrafish)	LOC100537262	XP_003201670.2
Homo sapiens (human)	USP30	NP_116052.2
Pan troglodytes (chimpanzee)	USP30	XP_001135207.1
Canis lupus familiaris (dog)	USP30	XP_005636387.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis for specific cleavage of Lys6-linked polyubiquitin chains by USP30.
Sato Y, Okatsu K, Saeki Y, Yamano K, Matsuda N, Kaiho A, Yamagata A, Goto-Ito S, Ishikawa M, Hashimoto Y, Tanaka K, Fukai S
Nature structural & molecular biology24(11)911-919(2017 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The distal ubiquitin-recognition mechanism of USP30 is similar to those of other USP family members, whereas Phe4 and Thr12 of the proximal ubiquitin are recognized by a USP30-specific surface.
Title: Structural basis for specific cleavage of Lys6-linked polyubiquitin chains by USP30.

The following USP30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00054	NM_001162484.1 Latest version!	Danio rerio ubiquitin specific peptidase 30 (usp30), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa10277	XM_005165156.4 Latest version!	Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa10277	XM_005165156.3	Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP30 ( NM_001162484.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001155956.1 Danio rerio ubiquitin specific peptidase 30 (usp30), mRNA.

Price & Availability↑

CloneID	ODa00054
Clone ID Related Accession (Same CDS sequence)	NM_001162484.1
Accession Version	NM_001162484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 30
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX511005.6. On Jun 26, 2009 this sequence version replaced XM_682406.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGAGAC TGACGTGGTC TAAAGAGGGC 
AGTCCCATAA AGAGACAGGA GCATGTTCAG TTTACAGAAT ATTTATCTCT GGATCGATAC 
AAACACTGTA GTGCTGCTCA GAGCCAGCAG AAGACCAGCA GAACAAATAA GGCCAAAGCT 
TCCGCAGACC CAAAGGACAA AGCCATTGCT AATGGTGTTG ATTCAGAGCA CTGTAATAAC 
AACAAGCCAC AGTCCAATGG AACTTTCCCC TCTGTCTTTC TGCACTCACC TGGTCTAAGC 
TCACAACTTA ACCTCACGTA CGACTACAGC ACCTCGGAGT ACCTCTTCCG CTTAACGGCG 
GTGCTGGTTC ATCATGGAGA CATGCATTCG GGTCATTTTA TCACTTACCG CCGCTGTCCT 
GCCGCTCCGC GTGGGACTTC TCCATTCAGC TCGCAGTGGC TGTGGGTGTC TGATGACTCT 
GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC TCCTCCAGTG CATATCTGCT CTTTTATGAG 
CGAATGCAGA GGCCTGGTCT GAGGGTAGAA GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155956.1
CDS	1..1476
Translation	MPWCKQGTTDKLVREFLRTGAAARNKMMKNWGVIGGIAAAMAAGVYVLWGPISDRRKKRKGMVPGLLNLGNTCFMNSLLQGLAACPSFIRWLEDFTSQNSADRERTERETQLSRSLMQLLKALSSHDPGEDDVLDAGGLLEALRLYRWHISSFEEQDAHELFHVLTSSLEEEQERQPRVAHLFDMQTLEKSVESKEKNISCRSGGPLHPIPSLWRTRHPFHGRLTSYMACKRCEQQSPVHYDSFDSLSLSIPSIQWGRPVTLDQCLQHFISSETIKEVECENCTKQQAGELVNGEVLESQRTTFVKQLKLGKRLTWSKEGSPIKRQEHVQFTEYLSLDRYKHCSAAQSQQKTSRTNKAKASADPKDKAIANGVDSEHCNNNKPQSNGTFPSVFLHSPGLSSQLNLTYDYSTSEYLFRLTAVLVHHGDMHSGHFITYRRCPAAPRGTSPFSSQWLWVSDDSVRKASLQEVLSSSAYLLFYERMQRPGLRVEE

Target ORF information:

RefSeq Version	NM_001162484.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 30 (usp30), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001162484.1

ORF Insert Sequence:

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGAGAC TGACGTGGTC TAAAGAGGGC 
AGTCCCATAA AGAGACAGGA GCATGTTCAG TTTACAGAAT ATTTATCTCT GGATCGATAC 
AAACACTGTA GTGCTGCTCA GAGCCAGCAG AAGACCAGCA GAACAAATAA GGCCAAAGCT 
TCCGCAGACC CAAAGGACAA AGCCATTGCT AATGGTGTTG ATTCAGAGCA CTGTAATAAC 
AACAAGCCAC AGTCCAATGG AACTTTCCCC TCTGTCTTTC TGCACTCACC TGGTCTAAGC 
TCACAACTTA ACCTCACGTA CGACTACAGC ACCTCGGAGT ACCTCTTCCG CTTAACGGCG 
GTGCTGGTTC ATCATGGAGA CATGCATTCG GGTCATTTTA TCACTTACCG CCGCTGTCCT 
GCCGCTCCGC GTGGGACTTC TCCATTCAGC TCGCAGTGGC TGTGGGTGTC TGATGACTCT 
GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC TCCTCCAGTG CATATCTGCT CTTTTATGAG 
CGAATGCAGA GGCCTGGTCT GAGGGTAGAA GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP30 ( XM_005165156.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165213.1 Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10277
Clone ID Related Accession (Same CDS sequence)	XM_005165156.4 , XM_005165156.3
Accession Version	XM_005165156.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGTTGC CTCAGTGCCT CTGCATCCAT 
TTGCAGAGAC TGACGTGGTC TAAAGAGGGC AGTCCCATAA AGAGACAGGA GCATGTTCAG 
TTTACAGAAT ATTTATCTCT GGATCGATAC AAACACTGTA GTGCTGCTCA GAGCCAGCAG 
AAGACCAGCA GAACAAATAA GGCCAAAGCT TCCGCAGACC CAAAGGACAA AGCCATTGCT 
AATGGTGTTG ATTCAGAGCA CTGTAATAAC AACAAGCCAC AGTCCAATGG AACTTTCCCC 
TCTGTCTTTC TGCACTCACC TGGTCTAAGC TCACAACTTA ACCTCACGTA CGACTACAGC 
ACCTCGGAGT ACCTCTTCCG CTTAACGGCG GTGCTGGTTC ATCATGGAGA CATGCATTCG 
GGTCATTTTA TCACTTACCG CCGCTGTCCT GCCGCTCCGC GTGGGACTTC TCCATTCAGC 
TCGCAGTGGC TGTGGGTGTC TGATGACTCT GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC 
TCCTCCAGTG CATATCTGCT CTTTTATGAG CGAATGCAGA GGCCTGGTCT GAGGGTAGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165213.1
CDS	555..2060
Translation	MPWCKQGTTDKLVREFLRTGAAARNKMMKNWGVIGGIAAAMAAGVYVLWGPISDRRKKRKGMVPGLLNLGNTCFMNSLLQGLAACPSFIRWLEDFTSQNSADRERTERETQLSRSLMQLLKALSSHDPGEDDVLDAGGLLEALRLYRWHISSFEEQDAHELFHVLTSSLEEEQERQPRVAHLFDMQTLEKSVESKEKNISCRSGGPLHPIPSLWRTRHPFHGRLTSYMACKRCEQQSPVHYDSFDSLSLSIPSIQWGRPVTLDQCLQHFISSETIKEVECENCTKQQAGELVNGEVLESQRTTFVKQLKLGKLPQCLCIHLQRLTWSKEGSPIKRQEHVQFTEYLSLDRYKHCSAAQSQQKTSRTNKAKASADPKDKAIANGVDSEHCNNNKPQSNGTFPSVFLHSPGLSSQLNLTYDYSTSEYLFRLTAVLVHHGDMHSGHFITYRRCPAAPRGTSPFSSQWLWVSDDSVRKASLQEVLSSSAYLLFYERMQRPGLRVEE

Target ORF information:

RefSeq Version	XM_005165156.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165156.4

ORF Insert Sequence:

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGTTGC CTCAGTGCCT CTGCATCCAT 
TTGCAGAGAC TGACGTGGTC TAAAGAGGGC AGTCCCATAA AGAGACAGGA GCATGTTCAG 
TTTACAGAAT ATTTATCTCT GGATCGATAC AAACACTGTA GTGCTGCTCA GAGCCAGCAG 
AAGACCAGCA GAACAAATAA GGCCAAAGCT TCCGCAGACC CAAAGGACAA AGCCATTGCT 
AATGGTGTTG ATTCAGAGCA CTGTAATAAC AACAAGCCAC AGTCCAATGG AACTTTCCCC 
TCTGTCTTTC TGCACTCACC TGGTCTAAGC TCACAACTTA ACCTCACGTA CGACTACAGC 
ACCTCGGAGT ACCTCTTCCG CTTAACGGCG GTGCTGGTTC ATCATGGAGA CATGCATTCG 
GGTCATTTTA TCACTTACCG CCGCTGTCCT GCCGCTCCGC GTGGGACTTC TCCATTCAGC 
TCGCAGTGGC TGTGGGTGTC TGATGACTCT GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC 
TCCTCCAGTG CATATCTGCT CTTTTATGAG CGAATGCAGA GGCCTGGTCT GAGGGTAGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP30 ( XM_005165156.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165213.1 Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10277
Clone ID Related Accession (Same CDS sequence)	XM_005165156.4 , XM_005165156.3
Accession Version	XM_005165156.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 30 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGTTGC CTCAGTGCCT CTGCATCCAT 
TTGCAGAGAC TGACGTGGTC TAAAGAGGGC AGTCCCATAA AGAGACAGGA GCATGTTCAG 
TTTACAGAAT ATTTATCTCT GGATCGATAC AAACACTGTA GTGCTGCTCA GAGCCAGCAG 
AAGACCAGCA GAACAAATAA GGCCAAAGCT TCCGCAGACC CAAAGGACAA AGCCATTGCT 
AATGGTGTTG ATTCAGAGCA CTGTAATAAC AACAAGCCAC AGTCCAATGG AACTTTCCCC 
TCTGTCTTTC TGCACTCACC TGGTCTAAGC TCACAACTTA ACCTCACGTA CGACTACAGC 
ACCTCGGAGT ACCTCTTCCG CTTAACGGCG GTGCTGGTTC ATCATGGAGA CATGCATTCG 
GGTCATTTTA TCACTTACCG CCGCTGTCCT GCCGCTCCGC GTGGGACTTC TCCATTCAGC 
TCGCAGTGGC TGTGGGTGTC TGATGACTCT GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC 
TCCTCCAGTG CATATCTGCT CTTTTATGAG CGAATGCAGA GGCCTGGTCT GAGGGTAGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165213.1
CDS	33..1538
Translation	MPWCKQGTTDKLVREFLRTGAAARNKMMKNWGVIGGIAAAMAAGVYVLWGPISDRRKKRKGMVPGLLNLGNTCFMNSLLQGLAACPSFIRWLEDFTSQNSADRERTERETQLSRSLMQLLKALSSHDPGEDDVLDAGGLLEALRLYRWHISSFEEQDAHELFHVLTSSLEEEQERQPRVAHLFDMQTLEKSVESKEKNISCRSGGPLHPIPSLWRTRHPFHGRLTSYMACKRCEQQSPVHYDSFDSLSLSIPSIQWGRPVTLDQCLQHFISSETIKEVECENCTKQQAGELVNGEVLESQRTTFVKQLKLGKLPQCLCIHLQRLTWSKEGSPIKRQEHVQFTEYLSLDRYKHCSAAQSQQKTSRTNKAKASADPKDKAIANGVDSEHCNNNKPQSNGTFPSVFLHSPGLSSQLNLTYDYSTSEYLFRLTAVLVHHGDMHSGHFITYRRCPAAPRGTSPFSSQWLWVSDDSVRKASLQEVLSSSAYLLFYERMQRPGLRVEE

Target ORF information:

RefSeq Version	XM_005165156.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 30 (usp30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165156.3

ORF Insert Sequence:

ATGCCTTGGT GCAAGCAAGG AACTACAGAC AAGCTTGTAC GAGAGTTTTT ACGCACTGGG 
GCCGCTGCAA GGAACAAGAT GATGAAGAAC TGGGGCGTGA TTGGTGGAAT AGCTGCTGCC 
ATGGCTGCTG GAGTCTATGT ACTATGGGGG CCAATTTCAG ACAGGAGGAA GAAGAGAAAA 
GGTATGGTTC CTGGTCTGCT TAATCTGGGG AACACGTGTT TTATGAACTC ACTTCTCCAG 
GGTCTGGCAG CGTGTCCTTC CTTCATCCGA TGGCTTGAAG ACTTCACAAG TCAAAACAGC 
GCTGATCGAG AGAGAACGGA AAGAGAAACT CAGCTGTCCA GATCTCTGAT GCAGTTACTT 
AAAGCCCTGT CGAGTCATGA TCCAGGAGAA GATGATGTTT TAGATGCCGG AGGCCTTTTG 
GAAGCGCTCA GACTTTACAG ATGGCACATC AGCTCATTCG AGGAACAGGA TGCTCATGAG 
CTCTTTCATG TTCTCACATC ATCATTAGAG GAAGAACAGG AACGCCAACC CAGAGTCGCT 
CACCTCTTCG ACATGCAAAC ACTGGAGAAA TCTGTGGAGT CCAAAGAGAA AAACATTAGT 
TGTCGAAGTG GAGGCCCACT GCATCCTATT CCTAGTTTAT GGAGAACTAG GCATCCTTTT 
CATGGACGGT TAACAAGTTA TATGGCTTGC AAGCGCTGCG AACAACAGAG TCCTGTGCAT 
TATGACTCTT TCGACAGCTT GTCTCTTTCG ATCCCTTCAA TACAGTGGGG TCGACCTGTT 
ACTTTGGACC AGTGTTTACA GCATTTCATC TCCTCTGAAA CCATCAAAGA AGTGGAATGT 
GAAAACTGCA CCAAGCAGCA GGCTGGAGAG CTAGTAAATG GAGAAGTGCT TGAGAGTCAG 
AGGACGACAT TTGTCAAGCA GCTGAAACTT GGAAAGTTGC CTCAGTGCCT CTGCATCCAT 
TTGCAGAGAC TGACGTGGTC TAAAGAGGGC AGTCCCATAA AGAGACAGGA GCATGTTCAG 
TTTACAGAAT ATTTATCTCT GGATCGATAC AAACACTGTA GTGCTGCTCA GAGCCAGCAG 
AAGACCAGCA GAACAAATAA GGCCAAAGCT TCCGCAGACC CAAAGGACAA AGCCATTGCT 
AATGGTGTTG ATTCAGAGCA CTGTAATAAC AACAAGCCAC AGTCCAATGG AACTTTCCCC 
TCTGTCTTTC TGCACTCACC TGGTCTAAGC TCACAACTTA ACCTCACGTA CGACTACAGC 
ACCTCGGAGT ACCTCTTCCG CTTAACGGCG GTGCTGGTTC ATCATGGAGA CATGCATTCG 
GGTCATTTTA TCACTTACCG CCGCTGTCCT GCCGCTCCGC GTGGGACTTC TCCATTCAGC 
TCGCAGTGGC TGTGGGTGTC TGATGACTCT GTGAGGAAGG CCAGTCTTCA GGAGGTGCTC 
TCCTCCAGTG CATATCTGCT CTTTTATGAG CGAATGCAGA GGCCTGGTCT GAGGGTAGAA 
GAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis for specific cleavage of Lys6-linked polyubiquitin chains by USP30.
Nature structural & molecular biology24(11)911-919(2017 Nov)
Sato Y,Okatsu K,Saeki Y,Yamano K,Matsuda N,Kaiho A,Yamagata A,Goto-Ito S,Ishikawa M,Hashimoto Y,Tanaka K,Fukai S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ

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USP30 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム