LGR4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LGR4
Entrez Gene ID	558825
Full Name	leucine-rich repeat containing G protein-coupled receptor 4
General protein information	Preferred Names leucine-rich repeat containing G protein-coupled receptor 4 Names leucine-rich repeat-containing G-protein coupled receptor 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001353862.1	NP_001340791.1	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
XM_021477865.1	XP_021333540.1	leucine-rich repeat-containing G-protein coupled receptor 4
XM_682092.8	XP_687184.4	leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1
XM_017357060.1	XP_017212549.1	leucine-rich repeat-containing G-protein coupled receptor 4 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LGR4	XP_005633820.1
Pan troglodytes (chimpanzee)	LGR4	XP_003313024.2
Homo sapiens (human)	LGR4	NP_060960.2
Rattus norvegicus (Norway rat)	Lgr4	NP_775450.1
	LGR4	XP_001090038.2
Bos taurus (cattle)	LGR4	NP_001192440.1
Mus musculus (house mouse)	Lgr4	NP_766259.2
Gallus gallus (chicken)	LGR4	XP_426162.3
Xenopus tropicalis (tropical clawed frog)	lgr4	NP_001119970.1
Danio rerio (zebrafish)	lgr4	XP_687184.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

An in?vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations.
Burrows JT, Pearson BJ, Scott IC
Stem cell reports4(4)670-84(2015 Apr)

Expression patterns of lgr4 and lgr6 during zebrafish development.
Hirose K, Shimoda N, Kikuchi Y
Gene expression patterns : GEP11(7)378-83(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lgr4 is expressed in the neural plate border, Kupffer's vesicle, neural tube, otic vesicles, midbrain, eyes, forebrain, and brain ventricular zone by 24h post-fertilization (hpf).
Title: Expression patterns of lgr4 and lgr6 during zebrafish development.

The following LGR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13152	NM_001353862.1 Latest version!	Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13152	XM_682092.8 Latest version!	Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa13152	XM_021477865.1 Latest version!	Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa59766	XM_017357060.1 Latest version!	Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LGR4 ( NM_001353862.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001340791.1 Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), mRNA.

Price & Availability↑

CloneID	ODa13152
Clone ID Related Accession (Same CDS sequence)	NM_001353862.1 , XM_021477865.1 , XM_682092.8
Accession Version	NM_001353862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511109.4. On Jul 27, 2017 this sequence version replaced XM_682092.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01014416.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340791.1
CDS	326..3241
Translation	MALLAVRMLVLGLCVGGQAAAAGEGQSTPATCSPLCRCDEDGGADCSGRGLTSVPTGLSAFTYYLDISMNNITELPANVFRNLPYLEELRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLPKLKELGFHSNNIASIPEGAFCRNSLLRTIHLFDNPLSFVGTTAFQNLSDLHSLMLRGASMMQDFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSLNSLASVPTAGLSALNQLKLTGNMELRNGLMSKTLPKLRSITVPYAYQCCAFVAYDSAVNPAEDDERRNAFGGEEDMERIPMVMHCSPLPGAFKPCEHLLGSWMIRLTVWFICLVALLFNCLVLAATFSPRTSSLSPSRFLVALLASANLLTGVYVAALTLLDTVTWGSFAEYGVWWETGAGCQVVGFLAVFSSEWAVLLLALAAVERCLAVRALMGKAGALRSRGERRERRRRFAIAALLLGLVSVAAACLSLYHGSAMGSPLCLPFSEGSSPGLGFTVALVLMNTLAYLLSAVVYTRLYCRLGRAQLADPEQAGSVRHIAWLIFTNCIFFCPVAAFSFAPLLAGTSNAVGGPEMAKSVTLIFFPLSACLNPVLYVCFSPSFRYDWLHLRGRGRTGGCGRLVAKTVTKGTVAGGSPVSDDGEGLSSDCGMYTKLHGDSRGMCEHCDAALHIRTSSSSGSSSSSACRHLVKSHSCPALMGNVPQCLSSEGYWPDTGTLSAQSEYGDEGDSFVSDSSEQVQACGRACFCQSRGLPLVHYSYNIPRMTD

Target ORF information:

RefSeq Version	NM_001353862.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353862.1

ORF Insert Sequence:

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( XM_021477865.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021333540.1 Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13152
Clone ID Related Accession (Same CDS sequence)	NM_001353862.1 , XM_021477865.1 , XM_682092.8
Accession Version	XM_021477865.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeat-containing G-protein coupled receptor 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333540.1
CDS	289..3204
Translation	MALLAVRMLVLGLCVGGQAAAAGEGQSTPATCSPLCRCDEDGGADCSGRGLTSVPTGLSAFTYYLDISMNNITELPANVFRNLPYLEELRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLPKLKELGFHSNNIASIPEGAFCRNSLLRTIHLFDNPLSFVGTTAFQNLSDLHSLMLRGASMMQDFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSLNSLASVPTAGLSALNQLKLTGNMELRNGLMSKTLPKLRSITVPYAYQCCAFVAYDSAVNPAEDDERRNAFGGEEDMERIPMVMHCSPLPGAFKPCEHLLGSWMIRLTVWFICLVALLFNCLVLAATFSPRTSSLSPSRFLVALLASANLLTGVYVAALTLLDTVTWGSFAEYGVWWETGAGCQVVGFLAVFSSEWAVLLLALAAVERCLAVRALMGKAGALRSRGERRERRRRFAIAALLLGLVSVAAACLSLYHGSAMGSPLCLPFSEGSSPGLGFTVALVLMNTLAYLLSAVVYTRLYCRLGRAQLADPEQAGSVRHIAWLIFTNCIFFCPVAAFSFAPLLAGTSNAVGGPEMAKSVTLIFFPLSACLNPVLYVCFSPSFRYDWLHLRGRGRTGGCGRLVAKTVTKGTVAGGSPVSDDGEGLSSDCGMYTKLHGDSRGMCEHCDAALHIRTSSSSGSSSSSACRHLVKSHSCPALMGNVPQCLSSEGYWPDTGTLSAQSEYGDEGDSFVSDSSEQVQACGRACFCQSRGLPLVHYSYNIPRMTD

Target ORF information:

RefSeq Version	XM_021477865.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477865.1

ORF Insert Sequence:

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( XM_682092.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_687184.4 Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13152
Clone ID Related Accession (Same CDS sequence)	NM_001353862.1 , XM_021477865.1 , XM_682092.8
Accession Version	XM_682092.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682092.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_687184.4
CDS	396..3311
Translation	MALLAVRMLVLGLCVGGQAAAAGEGQSTPATCSPLCRCDEDGGADCSGRGLTSVPTGLSAFTYYLDISMNNITELPANVFRNLPYLEELRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLPKLKELGFHSNNIASIPEGAFCRNSLLRTIHLFDNPLSFVGTTAFQNLSDLHSLMLRGASMMQDFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSLNSLASVPTAGLSALNQLKLTGNMELRNGLMSKTLPKLRSITVPYAYQCCAFVAYDSAVNPAEDDERRNAFGGEEDMERIPMVMHCSPLPGAFKPCEHLLGSWMIRLTVWFICLVALLFNCLVLAATFSPRTSSLSPSRFLVALLASANLLTGVYVAALTLLDTVTWGSFAEYGVWWETGAGCQVVGFLAVFSSEWAVLLLALAAVERCLAVRALMGKAGALRSRGERRERRRRFAIAALLLGLVSVAAACLSLYHGSAMGSPLCLPFSEGSSPGLGFTVALVLMNTLAYLLSAVVYTRLYCRLGRAQLADPEQAGSVRHIAWLIFTNCIFFCPVAAFSFAPLLAGTSNAVGGPEMAKSVTLIFFPLSACLNPVLYVCFSPSFRYDWLHLRGRGRTGGCGRLVAKTVTKGTVAGGSPVSDDGEGLSSDCGMYTKLHGDSRGMCEHCDAALHIRTSSSSGSSSSSACRHLVKSHSCPALMGNVPQCLSSEGYWPDTGTLSAQSEYGDEGDSFVSDSSEQVQACGRACFCQSRGLPLVHYSYNIPRMTD

Target ORF information:

RefSeq Version	XM_682092.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_682092.8

ORF Insert Sequence:

ATGGCATTGC TGGCCGTCCG GATGCTGGTG CTGGGGTTGT GTGTAGGGGG ACAGGCTGCC 
GCTGCGGGAG AGGGACAGTC TACTCCGGCC ACATGCTCTC CGCTGTGCCG CTGTGATGAG 
GACGGAGGAG CGGACTGCTC TGGACGGGGA CTGACCAGCG TCCCCACCGG CCTCAGCGCT 
TTCACCTACT ACCTGGACAT CAGCATGAAC AACATCACTG AGCTGCCTGC TAATGTCTTC 
AGAAACCTGC CGTATCTGGA AGAACTACGT TTGGCTGGTA ATGACCTTGC TTTCATCCAC 
CCAGAGGCTC TGTCTGGCCT CCATCAGCTT AAAGTCCTGA TGCTTCAGAA CAATCAGTTG 
AAGACTGTCC CCAGCGCAGC TCTCAAGAAT CTTAATGCCC TGCAGTCTTT GCGTTTGGAT 
GCTAACCACA TCACATCAGT GCCGGAGGAC AGTTTTGAGG GTCTGCAGCA GCTCAGGCAC 
CTCTGGTTGG ATGACAACAG CCTGACTGAG GTTCCCATCA GCCCCTTGCA GCACCAAAGT 
AACCTGCAGG CCCTGACGCT TGCCCTCAAC CGCATCACAC ACATCCCAGA CAACGCCTTC 
GCCAACCTCT CTAGCCTGGT TGTTTTGCAT CTCCACAATA ATCGGATCCA GGAAATTGGG 
AAAAACTGCT TTAATGGGCT GGACAACCTG GAGACATTGG ATCTAAACTT CAACAATCTG 
AAGATCTTCC CAGAGGCCAT TCAGATGCTG CCCAAGCTGA AAGAACTTGG ATTTCATAGC 
AACAACATTG CATCTATCCC TGAGGGAGCC TTCTGCAGGA ATTCCCTTCT TCGAACAATC 
CATCTTTTTG ACAACCCGCT CTCCTTCGTG GGAACCACTG CTTTCCAGAA CCTGTCAGAC 
TTGCACTCTC TAATGCTGCG TGGTGCCAGT ATGATGCAGG ATTTCCCCAG CCTCACTGGA 
ACAATAAATC TGGAGAGCCT GACTTTAACA GGAACTAAAA TCAGGAGCAT CCCGGCAGAC 
CTGTGTGAGG ATCTAACTGT GCTGCGAACA GTAGACCTTT CCTATAATGA CATTGAAGAC 
CTGCCATCCT TTCAGGGCTG CGTTAGACTG CAGGACATCA ATCTACAGCA TAACCAAATC 
AAGCAGATAG ACAGGGGAAC TTTCCAGGGC ATGACTAGCC TTCGAGTGCT AGACCTGAGC 
AGAAACCAGA TCAAGTTCAT CCACAGAGAT GCTTTCCTTT CTCTCAGCGC TCTCACTAAC 
CTGGATCTCA GTCTCAACTC CCTTGCCAGT GTTCCAACTG CAGGACTGAG CGCACTGAAC 
CAGCTCAAGC TGACTGGAAA CATGGAGCTG AGGAACGGCC TGATGTCTAA GACTCTGCCT 
AAACTCAGGT CCATAACGGT TCCTTATGCC TACCAGTGTT GCGCTTTTGT GGCTTATGAT 
AGTGCGGTTA ACCCAGCAGA GGATGACGAG CGAAGAAATG CATTTGGTGG CGAGGAGGAC 
ATGGAAAGGA TTCCTATGGT CATGCACTGC TCTCCATTAC CGGGGGCATT TAAACCTTGT 
GAACACTTGT TGGGAAGCTG GATGATTCGA CTGACTGTGT GGTTCATCTG CCTGGTTGCA 
CTCCTCTTCA ACTGCCTGGT CCTTGCCGCC ACCTTCTCCC CACGCACCTC CTCTCTATCC 
CCATCCCGCT TCCTGGTGGC TCTTCTGGCC TCTGCTAACC TGCTGACTGG GGTCTACGTG 
GCAGCGCTGA CCTTGCTGGA CACCGTCACC TGGGGCTCAT TTGCAGAGTA TGGTGTGTGG 
TGGGAAACAG GCGCTGGTTG TCAGGTGGTG GGCTTCCTCG CGGTGTTCTC CTCAGAGTGG 
GCCGTCCTGC TGCTGGCGTT AGCTGCTGTG GAGCGATGCT TGGCCGTGAG GGCTCTGATG 
GGGAAGGCGG GAGCTCTGAG GTCCAGGGGT GAAAGAAGGG AAAGGCGAAG GAGGTTTGCT 
ATAGCCGCAC TTCTTTTGGG ATTGGTATCT GTTGCAGCGG CCTGTCTTAG TCTGTACCAT 
GGATCAGCCA TGGGTTCTCC CCTCTGCCTG CCCTTCTCTG AAGGCTCCAG TCCTGGCCTG 
GGGTTTACAG TAGCCCTAGT GCTAATGAAC ACACTAGCAT ACTTGCTAAG CGCTGTGGTT 
TATACAAGGT TATACTGCAG ACTTGGGCGC GCTCAGCTGG CTGACCCTGA GCAGGCTGGA 
TCTGTGCGCC ACATCGCTTG GCTCATCTTC ACCAACTGCA TCTTCTTCTG CCCGGTGGCA 
GCTTTCTCCT TTGCTCCCCT GCTGGCCGGG ACTAGTAATG CAGTGGGCGG ACCAGAGATG 
GCCAAATCAG TGACGCTCAT ATTCTTCCCT CTGTCTGCCT GCTTGAACCC TGTGCTGTAC 
GTCTGCTTCA GTCCTTCCTT CAGGTATGAC TGGCTTCACC TTCGAGGACG GGGCAGGACT 
GGAGGATGCG GCAGGCTGGT TGCTAAAACA GTCACCAAAG GAACTGTGGC AGGAGGATCA 
CCAGTTTCTG ATGATGGAGA AGGCTTGTCA AGTGATTGTG GAATGTACAC AAAGCTTCAC 
GGCGACTCTC GAGGAATGTG CGAACATTGT GACGCCGCTC TCCACATCAG GACGTCTTCC 
TCTTCAGGTT CCTCCTCTTC ATCGGCCTGC AGACATTTAG TAAAGTCCCA CAGCTGTCCT 
GCTTTGATGG GGAACGTGCC TCAGTGTTTG AGTTCAGAGG GCTACTGGCC CGATACCGGG 
ACGCTTTCCG CACAGTCGGA ATATGGAGAC GAGGGGGATT CGTTCGTATC AGACAGTTCA 
GAGCAGGTGC AGGCGTGCGG CCGAGCTTGT TTTTGCCAGA GCCGCGGCCT CCCTTTAGTA 
CACTACTCTT ACAACATCCC CCGCATGACA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGR4 ( XM_017357060.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212549.1 Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59766
Clone ID Related Accession (Same CDS sequence)	XM_017357060.1
Accession Version	XM_017357060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeat-containing G-protein coupled receptor 4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCAG TTTGGCTTTT GAAGCTACAT CATTCATTGT GTATTAATGG CGTTGAAAGA 
ACAAACACGT GCCATTTTAC CAAATTCTGG AGTGCCTCTG TCAGTACTTC TGAAGTCAGT 
TTTGAACTCT GGCTGGGTGT GTCTGATGTT TTTGCATGTT TGTCTTTTCT TCGCTCATCC 
CGCTTCTGCC CATCTGCGGG AGGACCAGAT AACATCTGTC TAAGTGATAT CAGACGTTTG 
GCTGGTAATG ACCTTGCTTT CATCCACCCA GAGGCTCTGT CTGGCCTCCA TCAGCTTAAA 
GTCCTGATGC TTCAGAACAA TCAGTTGAAG ACTGTCCCCA GCGCAGCTCT CAAGAATCTT 
AATGCCCTGC AGTCTTTGCG TTTGGATGCT AACCACATCA CATCAGTGCC GGAGGACAGT 
TTTGAGGGTC TGCAGCAGCT CAGGCACCTC TGGTTGGATG ACAACAGCCT GACTGAGGTT 
CCCATCAGCC CCTTGCAGCA CCAAAGTAAC CTGCAGGCCC TGACGCTTGC CCTCAACCGC 
ATCACACACA TCCCAGACAA CGCCTTCGCC AACCTCTCTA GCCTGGTTGT TTTGCATCTC 
CACAATAATC GGATCCAGGA AATTGGGAAA AACTGCTTTA ATGGGCTGGA CAACCTGGAG 
ACATTGGATC TAAACTTCAA CAATCTGAAG ATCTTCCCAG AGGCCATTCA GATGCTGCCC 
AAGCTGAAAG AACTTGGATT TCATAGCAAC AACATTGCAT CTATCCCTGA GGGAGCCTTC 
TGCAGGAATT CCCTTCTTCG AACAATCCAT CTTTTTGACA ACCCGCTCTC CTTCGTGGGA 
ACCACTGCTT TCCAGAACCT GTCAGACTTG CACTCTCTAA TGCTGCGTGG TGCCAGTATG 
ATGCAGGATT TCCCCAGCCT CACTGGAACA ATAAATCTGG AGAGCCTGAC TTTAACAGGA 
ACTAAAATCA GGAGCATCCC GGCAGACCTG TGTGAGGATC TAACTGTGCT GCGAACAGTA 
GACCTTTCCT ATAATGACAT TGAAGACCTG CCATCCTTTC AGGGCTGCGT TAGACTGCAG 
GACATCAATC TACAGCATAA CCAAATCAAG CAGATAGACA GGGGAACTTT CCAGGGCATG 
ACTAGCCTTC GAGTGCTAGA CCTGAGCAGA AACCAGATCA AGTTCATCCA CAGAGATGCT 
TTCCTTTCTC TCAGCGCTCT CACTAACCTG GATCTCAGTC TCAACTCCCT TGCCAGTGTT 
CCAACTGCAG GACTGAGCGC ACTGAACCAG CTCAAGCTGA CTGGAAACAT GGAGCTGAGG 
AACGGCCTGA TGTCTAAGAC TCTGCCTAAA CTCAGGTCCA TAACGGTTCC TTATGCCTAC 
CAGTGTTGCG CTTTTGTGGC TTATGATAGT GCGGTTAACC CAGCAGAGGA TGACGAGCGA 
AGAAATGCAT TTGGTGGCGA GGAGGACATG GAAAGGATTC CTATGGTCAT GCACTGCTCT 
CCATTACCGG GGGCATTTAA ACCTTGTGAA CACTTGTTGG GAAGCTGGAT GATTCGACTG 
ACTGTGTGGT TCATCTGCCT GGTTGCACTC CTCTTCAACT GCCTGGTCCT TGCCGCCACC 
TTCTCCCCAC GCACCTCCTC TCTATCCCCA TCCCGCTTCC TGGTGGCTCT TCTGGCCTCT 
GCTAACCTGC TGACTGGGGT CTACGTGGCA GCGCTGACCT TGCTGGACAC CGTCACCTGG 
GGCTCATTTG CAGAGTATGG TGTGTGGTGG GAAACAGGCG CTGGTTGTCA GGTGGTGGGC 
TTCCTCGCGG TGTTCTCCTC AGAGTGGGCC GTCCTGCTGC TGGCGTTAGC TGCTGTGGAG 
CGATGCTTGG CCGTGAGGGC TCTGATGGGG AAGGCGGGAG CTCTGAGGTC CAGGGGTGAA 
AGAAGGGAAA GGCGAAGGAG GTTTGCTATA GCCGCACTTC TTTTGGGATT GGTATCTGTT 
GCAGCGGCCT GTCTTAGTCT GTACCATGGA TCAGCCATGG GTTCTCCCCT CTGCCTGCCC 
TTCTCTGAAG GCTCCAGTCC TGGCCTGGGG TTTACAGTAG CCCTAGTGCT AATGAACACA 
CTAGCATACT TGCTAAGCGC TGTGGTTTAT ACAAGGTTAT ACTGCAGACT TGGGCGCGCT 
CAGCTGGCTG ACCCTGAGCA GGCTGGATCT GTGCGCCACA TCGCTTGGCT CATCTTCACC 
AACTGCATCT TCTTCTGCCC GGTGGCAGCT TTCTCCTTTG CTCCCCTGCT GGCCGGGACT 
AGTAATGCAG TGGGCGGACC AGAGATGGCC AAATCAGTGA CGCTCATATT CTTCCCTCTG 
TCTGCCTGCT TGAACCCTGT GCTGTACGTC TGCTTCAGTC CTTCCTTCAG GTATGACTGG 
CTTCACCTTC GAGGACGGGG CAGGACTGGA GGATGCGGCA GGCTGGTTGC TAAAACAGTC 
ACCAAAGGAA CTGTGGCAGG AGGATCACCA GTTTCTGATG ATGGAGAAGG CTTGTCAAGT 
GATTGTGGAA TGTACACAAA GCTTCACGGC GACTCTCGAG GAATGTGCGA ACATTGTGAC 
GCCGCTCTCC ACATCAGGAC GTCTTCCTCT TCAGGTTCCT CCTCTTCATC GGCCTGCAGA 
CATTTAGTAA AGTCCCACAG CTGTCCTGCT TTGATGGGGA ACGTGCCTCA GTGTTTGAGT 
TCAGAGGGCT ACTGGCCCGA TACCGGGACG CTTTCCGCAC AGTCGGAATA TGGAGACGAG 
GGGGATTCGT TCGTATCAGA CAGTTCAGAG CAGGTGCAGG CGTGCGGCCG AGCTTGTTTT 
TGCCAGAGCC GCGGCCTCCC TTTAGTACAC TACTCTTACA ACATCCCCCG CATGACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212549.1
CDS	16..2898
Translation	MSPVWLLKLHHSLCINGVERTNTCHFTKFWSASVSTSEVSFELWLGVSDVFACLSFLRSSRFCPSAGGPDNICLSDIRRLAGNDLAFIHPEALSGLHQLKVLMLQNNQLKTVPSAALKNLNALQSLRLDANHITSVPEDSFEGLQQLRHLWLDDNSLTEVPISPLQHQSNLQALTLALNRITHIPDNAFANLSSLVVLHLHNNRIQEIGKNCFNGLDNLETLDLNFNNLKIFPEAIQMLPKLKELGFHSNNIASIPEGAFCRNSLLRTIHLFDNPLSFVGTTAFQNLSDLHSLMLRGASMMQDFPSLTGTINLESLTLTGTKIRSIPADLCEDLTVLRTVDLSYNDIEDLPSFQGCVRLQDINLQHNQIKQIDRGTFQGMTSLRVLDLSRNQIKFIHRDAFLSLSALTNLDLSLNSLASVPTAGLSALNQLKLTGNMELRNGLMSKTLPKLRSITVPYAYQCCAFVAYDSAVNPAEDDERRNAFGGEEDMERIPMVMHCSPLPGAFKPCEHLLGSWMIRLTVWFICLVALLFNCLVLAATFSPRTSSLSPSRFLVALLASANLLTGVYVAALTLLDTVTWGSFAEYGVWWETGAGCQVVGFLAVFSSEWAVLLLALAAVERCLAVRALMGKAGALRSRGERRERRRRFAIAALLLGLVSVAAACLSLYHGSAMGSPLCLPFSEGSSPGLGFTVALVLMNTLAYLLSAVVYTRLYCRLGRAQLADPEQAGSVRHIAWLIFTNCIFFCPVAAFSFAPLLAGTSNAVGGPEMAKSVTLIFFPLSACLNPVLYVCFSPSFRYDWLHLRGRGRTGGCGRLVAKTVTKGTVAGGSPVSDDGEGLSSDCGMYTKLHGDSRGMCEHCDAALHIRTSSSSGSSSSSACRHLVKSHSCPALMGNVPQCLSSEGYWPDTGTLSAQSEYGDEGDSFVSDSSEQVQACGRACFCQSRGLPLVHYSYNIPRMTD

Target ORF information:

RefSeq Version	XM_017357060.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine rich repeat containing G protein-coupled receptor 4 (lgr4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357060.1

ORF Insert Sequence:

ATGTCACCAG TTTGGCTTTT GAAGCTACAT CATTCATTGT GTATTAATGG CGTTGAAAGA 
ACAAACACGT GCCATTTTAC CAAATTCTGG AGTGCCTCTG TCAGTACTTC TGAAGTCAGT 
TTTGAACTCT GGCTGGGTGT GTCTGATGTT TTTGCATGTT TGTCTTTTCT TCGCTCATCC 
CGCTTCTGCC CATCTGCGGG AGGACCAGAT AACATCTGTC TAAGTGATAT CAGACGTTTG 
GCTGGTAATG ACCTTGCTTT CATCCACCCA GAGGCTCTGT CTGGCCTCCA TCAGCTTAAA 
GTCCTGATGC TTCAGAACAA TCAGTTGAAG ACTGTCCCCA GCGCAGCTCT CAAGAATCTT 
AATGCCCTGC AGTCTTTGCG TTTGGATGCT AACCACATCA CATCAGTGCC GGAGGACAGT 
TTTGAGGGTC TGCAGCAGCT CAGGCACCTC TGGTTGGATG ACAACAGCCT GACTGAGGTT 
CCCATCAGCC CCTTGCAGCA CCAAAGTAAC CTGCAGGCCC TGACGCTTGC CCTCAACCGC 
ATCACACACA TCCCAGACAA CGCCTTCGCC AACCTCTCTA GCCTGGTTGT TTTGCATCTC 
CACAATAATC GGATCCAGGA AATTGGGAAA AACTGCTTTA ATGGGCTGGA CAACCTGGAG 
ACATTGGATC TAAACTTCAA CAATCTGAAG ATCTTCCCAG AGGCCATTCA GATGCTGCCC 
AAGCTGAAAG AACTTGGATT TCATAGCAAC AACATTGCAT CTATCCCTGA GGGAGCCTTC 
TGCAGGAATT CCCTTCTTCG AACAATCCAT CTTTTTGACA ACCCGCTCTC CTTCGTGGGA 
ACCACTGCTT TCCAGAACCT GTCAGACTTG CACTCTCTAA TGCTGCGTGG TGCCAGTATG 
ATGCAGGATT TCCCCAGCCT CACTGGAACA ATAAATCTGG AGAGCCTGAC TTTAACAGGA 
ACTAAAATCA GGAGCATCCC GGCAGACCTG TGTGAGGATC TAACTGTGCT GCGAACAGTA 
GACCTTTCCT ATAATGACAT TGAAGACCTG CCATCCTTTC AGGGCTGCGT TAGACTGCAG 
GACATCAATC TACAGCATAA CCAAATCAAG CAGATAGACA GGGGAACTTT CCAGGGCATG 
ACTAGCCTTC GAGTGCTAGA CCTGAGCAGA AACCAGATCA AGTTCATCCA CAGAGATGCT 
TTCCTTTCTC TCAGCGCTCT CACTAACCTG GATCTCAGTC TCAACTCCCT TGCCAGTGTT 
CCAACTGCAG GACTGAGCGC ACTGAACCAG CTCAAGCTGA CTGGAAACAT GGAGCTGAGG 
AACGGCCTGA TGTCTAAGAC TCTGCCTAAA CTCAGGTCCA TAACGGTTCC TTATGCCTAC 
CAGTGTTGCG CTTTTGTGGC TTATGATAGT GCGGTTAACC CAGCAGAGGA TGACGAGCGA 
AGAAATGCAT TTGGTGGCGA GGAGGACATG GAAAGGATTC CTATGGTCAT GCACTGCTCT 
CCATTACCGG GGGCATTTAA ACCTTGTGAA CACTTGTTGG GAAGCTGGAT GATTCGACTG 
ACTGTGTGGT TCATCTGCCT GGTTGCACTC CTCTTCAACT GCCTGGTCCT TGCCGCCACC 
TTCTCCCCAC GCACCTCCTC TCTATCCCCA TCCCGCTTCC TGGTGGCTCT TCTGGCCTCT 
GCTAACCTGC TGACTGGGGT CTACGTGGCA GCGCTGACCT TGCTGGACAC CGTCACCTGG 
GGCTCATTTG CAGAGTATGG TGTGTGGTGG GAAACAGGCG CTGGTTGTCA GGTGGTGGGC 
TTCCTCGCGG TGTTCTCCTC AGAGTGGGCC GTCCTGCTGC TGGCGTTAGC TGCTGTGGAG 
CGATGCTTGG CCGTGAGGGC TCTGATGGGG AAGGCGGGAG CTCTGAGGTC CAGGGGTGAA 
AGAAGGGAAA GGCGAAGGAG GTTTGCTATA GCCGCACTTC TTTTGGGATT GGTATCTGTT 
GCAGCGGCCT GTCTTAGTCT GTACCATGGA TCAGCCATGG GTTCTCCCCT CTGCCTGCCC 
TTCTCTGAAG GCTCCAGTCC TGGCCTGGGG TTTACAGTAG CCCTAGTGCT AATGAACACA 
CTAGCATACT TGCTAAGCGC TGTGGTTTAT ACAAGGTTAT ACTGCAGACT TGGGCGCGCT 
CAGCTGGCTG ACCCTGAGCA GGCTGGATCT GTGCGCCACA TCGCTTGGCT CATCTTCACC 
AACTGCATCT TCTTCTGCCC GGTGGCAGCT TTCTCCTTTG CTCCCCTGCT GGCCGGGACT 
AGTAATGCAG TGGGCGGACC AGAGATGGCC AAATCAGTGA CGCTCATATT CTTCCCTCTG 
TCTGCCTGCT TGAACCCTGT GCTGTACGTC TGCTTCAGTC CTTCCTTCAG GTATGACTGG 
CTTCACCTTC GAGGACGGGG CAGGACTGGA GGATGCGGCA GGCTGGTTGC TAAAACAGTC 
ACCAAAGGAA CTGTGGCAGG AGGATCACCA GTTTCTGATG ATGGAGAAGG CTTGTCAAGT 
GATTGTGGAA TGTACACAAA GCTTCACGGC GACTCTCGAG GAATGTGCGA ACATTGTGAC 
GCCGCTCTCC ACATCAGGAC GTCTTCCTCT TCAGGTTCCT CCTCTTCATC GGCCTGCAGA 
CATTTAGTAA AGTCCCACAG CTGTCCTGCT TTGATGGGGA ACGTGCCTCA GTGTTTGAGT 
TCAGAGGGCT ACTGGCCCGA TACCGGGACG CTTTCCGCAC AGTCGGAATA TGGAGACGAG 
GGGGATTCGT TCGTATCAGA CAGTTCAGAG CAGGTGCAGG CGTGCGGCCG AGCTTGTTTT 
TGCCAGAGCC GCGGCCTCCC TTTAGTACAC TACTCTTACA ACATCCCCCG CATGACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

An in?vivo requirement for the mediator subunit med14 in the maintenance of stem cell populations.
Stem cell reports4(4)670-84(2015 Apr)
Burrows JT,Pearson BJ,Scott IC

Expression patterns of lgr4 and lgr6 during zebrafish development.
Gene expression patterns : GEP11(7)378-83(2011 Oct)
Hirose K,Shimoda N,Kikuchi Y

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LGR4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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