lemd3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lemd3
Entrez Gene ID	558474
Full Name	LEM domain containing 3
Synonyms	im:7158446,si:dkey-6a5.6,wu:fb34b12
General protein information	Preferred Names LEM domain containing 3 Names inner nuclear membrane protein Man1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044864.1	NP_001038329.1	inner nuclear membrane protein Man1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LEMD3	XP_005625712.1
Bos taurus (cattle)	LEMD3	NP_001179628.1
Homo sapiens (human)	LEMD3	NP_055134.2
Pan troglodytes (chimpanzee)	LEMD3	XP_509197.2
	LEMD3	XP_001116983.1
Mus musculus (house mouse)	Lemd3	NP_001074662.2
Rattus norvegicus (Norway rat)	Lemd3	NP_001177929.1
Gallus gallus (chicken)	LEMD3	XP_416073.3
Danio rerio (zebrafish)	lemd3	NP_001038329.1
Xenopus tropicalis (tropical clawed frog)	lemd3	NP_001136130.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lemd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lemd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31398	NM_001044864.1 Latest version!	Danio rerio LEM domain containing 3 (lemd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lemd3 ( NM_001044864.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038329.1 Danio rerio LEM domain containing 3 (lemd3), mRNA.

Price & Availability↑

CloneID	ODa31398
Clone ID Related Accession (Same CDS sequence)	NM_001044864.1
Accession Version	NM_001044864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	inner nuclear membrane protein Man1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX119963.5. On Sep 1, 2006 this sequence version replaced XM_681690.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01014346.1, BC168502.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCTG CACAGTTAAC GGATGAGGAG CTTGTGTCTG AATTGAAACG GCTCGGCTTC 
ACCCCGGGCC CGGTGACGGA GAGCACGCGG CCCGTGTACT TGAAAAAACT GAAAAAGCTG 
CGGGAAGAGC AGCCACAACT GCGGGGCTCC CGGAGCGGGA AAGGGCGCAG CAGCAGCGGA 
ACTATCAACA ACAACGGCAG CGGCGCTAGC AGCAACAGCA GCTCGGCGGC GCGAGCTCCG 
GGATTCAGCG CCAGGCCACC GACCAGTGAC GTCACGCGCC TGAGCGCCAG CCGGAGCGAA 
AAGCGCAAAG CCGGAAAGTT CGTGCTGGGC TTTAGCTCGG ACGAGTCGGA CGTAGAGGCT 
CCGCAGAAAA AAGGAGGCCC GAATCACGGC GGCAGCAGGA GAGACCGGGG ATCCGCACAG 
CAGACACCCA CCATCAGGCC TGCGGGAACA CCCGCTGCCG CCGTCTCCCT CGGGCTATCC 
GCGGACAGGA AAAGCGTTTC TGGCTCTCCG TGGTGGGCAG ACCGGGGGAA ATCGGGTGAT 
TTAGCGGAAA CGGAAAGAGA CAGTCGGGCT GTGAATGGGA ATAAGGCTTT TTACGAGTCG 
AACAGTAGTA AGTTAGCCAG GGATTACTCT GATTCCGACG AGGAGGAGGA AGAGGTGGGT 
GAACGAGACC GTCCGCGCGA ACGCCGTCTG ACTTCCAGAC ACAGTCCACC CGCCTCTTCT 
TCCCCTTACC GGAGCGCCTG GAGCTCGGAT AAGACGGCGC TGGGCGCGCA TGATACACCA 
GACTACAACC TAGATATGGT CGGGGAACGG AGGGACGAGG ATGACAAGGA ACCTAGCGAG 
TTTAGCGGAA GCTACGTGAG CCGCAGCTAC CCGAAACTGT CCGGAACGGG CAGGGATGAC 
AACAGCGGTG GGAGCAGCAT CCCGTCTGGT TCTTTCCATA GGAATCACCT CGACAATGGA 
GACGGCGAAT CCGCCTGCAG CAGCCGGTTC AGCATCGGTC TCAGACCACG CTTTCCTTCC 
TACACCGCGA CTTACCGAGC GAACCACTCG AACCACACCG GTTCCAATCA CTCCTATAGC 
CATGCTTCAG CTCACAAGTC CAAGCAGCAC ACCACGGTCC CGGAGGACGA GCTCCTGCAG 
CAGTTTAAGC GGGAGGAGGT GTCCTCCACC GGCGGCTTCA GTGCCCACTA CTTGTCCATG 
TTCCTCCTGA CAGCCGCCTG CCTGTTCTTC GTGCTGCTGG GCCTCATGTA CCTGAGGATG 
AGGGGCTCCG GCACCGCCGT GGGTGATGCA GTCATCATGC AGCATCCATT CGGAGCTGAG 
TTTGATAAGC AGTATGACGT TAAAGAGCGA GAACTCATCC TTAACCTTCT CTTGTCTCTA 
CATGAGCACC TGGCCAGTGT TGCAGGAGAA CATGATTGTG GAGATTTTAA AAATCCTTTA 
AACAGAAGTG TCTCTTTTAG TGACGCCTCC AAGTACCTCA AGCTCCAAAA TGAAGCCTAT 
GAAAACTTGA TTGAAACGTC TTTAGAATGG GTCATGAAAA CCAAAGATGT TGTTGGAATA 
AGGTTGATTG GTGGTGACCC TGATCATCCC ATAGAGGATG TATCTGAAAT CTCTCGTCTG 
GAGTCCATCC ACCCCCGCAT GTCCTTCTTC TGCCGCTTCA GACGAGCCTT CTTCACTGTG 
ATCTACAGAG TTCTCCTCAT CCTCGCAGGC ATCGGTGTTG TTGTGGGTTT GGTGTATTAC 
ATGAAATACA GATGGCGCAA GGAAGAGGAG GAAACCAGAC ATATGTATGA CATGGTGGAG 
CGAATAATCG ATGTCCTGAG GAGTCACAAT GAGGCCTGTC AGGAGAATAA GGACTTGCAG 
GCATACCTGC CCATCCCTCA CGTCCGTGAC TCTCTTGTCC AGCCTCAGGA GAGAAAGAGG 
ATGAAGAAAG TGTGGGACAG AGCAGTTTCA TTCCTCTCTG CGAATGAGTC CCGCATCCGG 
ACAGAGAAGC AGAGGATTGG AGGGGCGGAT TTCATGGTGT GGCGGTGGCT TCAGCCCTCA 
ATGTCACCTG ATAAGATGTG CAACATGCCC TCCAAAGTGT GGCAAGGCCA AGCATTTCCG 
CTGGACCGAA GAAATTCGCC ACCAAACAGC TTAACGCCAT GCTTGAAGAT TCGCAACATG 
TTTGATCCTG TAATGGAAGT GGGGGAAAAC TGGCATCTCG CCATTCAAGA AGCCATTTTG 
GAGAAGTGCA GTGATAATGA TGGAATCGTT CACATAGCTG TTGACAAAAA CTCAAGAGAG 
GGCTGCGTTT ACGTGAAGTG CCTTTCAGCA GAGCATTCTG GGAAAGCCTT CAAAGCGCTC 
CACGGCTCCT GGTTCGATGG TAAGCTGGTG ACGGTCAAGT ACCTGCGCCT GGATCGCTAC 
CACCAGCGTT TCCCACAAGC CCTAGGCTCC AACACACCTC TCAAACCCTC CAGCTCCAGC 
ATGAACAGCA TGTCTCGTCT GAACCAGCGC TCCAGCACGT CCAGCTTGAA GGACACGTTC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038329.1
CDS	8..2533
Translation	MASAQLTDEELVSELKRLGFTPGPVTESTRPVYLKKLKKLREEQPQLRGSRSGKGRSSSGTINNNGSGASSNSSSAARAPGFSARPPTSDVTRLSASRSEKRKAGKFVLGFSSDESDVEAPQKKGGPNHGGSRRDRGSAQQTPTIRPAGTPAAAVSLGLSADRKSVSGSPWWADRGKSGDLAETERDSRAVNGNKAFYESNSSKLARDYSDSDEEEEEVGERDRPRERRLTSRHSPPASSSPYRSAWSSDKTALGAHDTPDYNLDMVGERRDEDDKEPSEFSGSYVSRSYPKLSGTGRDDNSGGSSIPSGSFHRNHLDNGDGESACSSRFSIGLRPRFPSYTATYRANHSNHTGSNHSYSHASAHKSKQHTTVPEDELLQQFKREEVSSTGGFSAHYLSMFLLTAACLFFVLLGLMYLRMRGSGTAVGDAVIMQHPFGAEFDKQYDVKERELILNLLLSLHEHLASVAGEHDCGDFKNPLNRSVSFSDASKYLKLQNEAYENLIETSLEWVMKTKDVVGIRLIGGDPDHPIEDVSEISRLESIHPRMSFFCRFRRAFFTVIYRVLLILAGIGVVVGLVYYMKYRWRKEEEETRHMYDMVERIIDVLRSHNEACQENKDLQAYLPIPHVRDSLVQPQERKRMKKVWDRAVSFLSANESRIRTEKQRIGGADFMVWRWLQPSMSPDKMCNMPSKVWQGQAFPLDRRNSPPNSLTPCLKIRNMFDPVMEVGENWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLSAEHSGKAFKALHGSWFDGKLVTVKYLRLDRYHQRFPQALGSNTPLKPSSSSMNSMSRLNQRSSTSSLKDTFS

Target ORF information:

RefSeq Version	NM_001044864.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio LEM domain containing 3 (lemd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044864.1

ORF Insert Sequence:

ATGGCGTCTG CACAGTTAAC GGATGAGGAG CTTGTGTCTG AATTGAAACG GCTCGGCTTC 
ACCCCGGGCC CGGTGACGGA GAGCACGCGG CCCGTGTACT TGAAAAAACT GAAAAAGCTG 
CGGGAAGAGC AGCCACAACT GCGGGGCTCC CGGAGCGGGA AAGGGCGCAG CAGCAGCGGA 
ACTATCAACA ACAACGGCAG CGGCGCTAGC AGCAACAGCA GCTCGGCGGC GCGAGCTCCG 
GGATTCAGCG CCAGGCCACC GACCAGTGAC GTCACGCGCC TGAGCGCCAG CCGGAGCGAA 
AAGCGCAAAG CCGGAAAGTT CGTGCTGGGC TTTAGCTCGG ACGAGTCGGA CGTAGAGGCT 
CCGCAGAAAA AAGGAGGCCC GAATCACGGC GGCAGCAGGA GAGACCGGGG ATCCGCACAG 
CAGACACCCA CCATCAGGCC TGCGGGAACA CCCGCTGCCG CCGTCTCCCT CGGGCTATCC 
GCGGACAGGA AAAGCGTTTC TGGCTCTCCG TGGTGGGCAG ACCGGGGGAA ATCGGGTGAT 
TTAGCGGAAA CGGAAAGAGA CAGTCGGGCT GTGAATGGGA ATAAGGCTTT TTACGAGTCG 
AACAGTAGTA AGTTAGCCAG GGATTACTCT GATTCCGACG AGGAGGAGGA AGAGGTGGGT 
GAACGAGACC GTCCGCGCGA ACGCCGTCTG ACTTCCAGAC ACAGTCCACC CGCCTCTTCT 
TCCCCTTACC GGAGCGCCTG GAGCTCGGAT AAGACGGCGC TGGGCGCGCA TGATACACCA 
GACTACAACC TAGATATGGT CGGGGAACGG AGGGACGAGG ATGACAAGGA ACCTAGCGAG 
TTTAGCGGAA GCTACGTGAG CCGCAGCTAC CCGAAACTGT CCGGAACGGG CAGGGATGAC 
AACAGCGGTG GGAGCAGCAT CCCGTCTGGT TCTTTCCATA GGAATCACCT CGACAATGGA 
GACGGCGAAT CCGCCTGCAG CAGCCGGTTC AGCATCGGTC TCAGACCACG CTTTCCTTCC 
TACACCGCGA CTTACCGAGC GAACCACTCG AACCACACCG GTTCCAATCA CTCCTATAGC 
CATGCTTCAG CTCACAAGTC CAAGCAGCAC ACCACGGTCC CGGAGGACGA GCTCCTGCAG 
CAGTTTAAGC GGGAGGAGGT GTCCTCCACC GGCGGCTTCA GTGCCCACTA CTTGTCCATG 
TTCCTCCTGA CAGCCGCCTG CCTGTTCTTC GTGCTGCTGG GCCTCATGTA CCTGAGGATG 
AGGGGCTCCG GCACCGCCGT GGGTGATGCA GTCATCATGC AGCATCCATT CGGAGCTGAG 
TTTGATAAGC AGTATGACGT TAAAGAGCGA GAACTCATCC TTAACCTTCT CTTGTCTCTA 
CATGAGCACC TGGCCAGTGT TGCAGGAGAA CATGATTGTG GAGATTTTAA AAATCCTTTA 
AACAGAAGTG TCTCTTTTAG TGACGCCTCC AAGTACCTCA AGCTCCAAAA TGAAGCCTAT 
GAAAACTTGA TTGAAACGTC TTTAGAATGG GTCATGAAAA CCAAAGATGT TGTTGGAATA 
AGGTTGATTG GTGGTGACCC TGATCATCCC ATAGAGGATG TATCTGAAAT CTCTCGTCTG 
GAGTCCATCC ACCCCCGCAT GTCCTTCTTC TGCCGCTTCA GACGAGCCTT CTTCACTGTG 
ATCTACAGAG TTCTCCTCAT CCTCGCAGGC ATCGGTGTTG TTGTGGGTTT GGTGTATTAC 
ATGAAATACA GATGGCGCAA GGAAGAGGAG GAAACCAGAC ATATGTATGA CATGGTGGAG 
CGAATAATCG ATGTCCTGAG GAGTCACAAT GAGGCCTGTC AGGAGAATAA GGACTTGCAG 
GCATACCTGC CCATCCCTCA CGTCCGTGAC TCTCTTGTCC AGCCTCAGGA GAGAAAGAGG 
ATGAAGAAAG TGTGGGACAG AGCAGTTTCA TTCCTCTCTG CGAATGAGTC CCGCATCCGG 
ACAGAGAAGC AGAGGATTGG AGGGGCGGAT TTCATGGTGT GGCGGTGGCT TCAGCCCTCA 
ATGTCACCTG ATAAGATGTG CAACATGCCC TCCAAAGTGT GGCAAGGCCA AGCATTTCCG 
CTGGACCGAA GAAATTCGCC ACCAAACAGC TTAACGCCAT GCTTGAAGAT TCGCAACATG 
TTTGATCCTG TAATGGAAGT GGGGGAAAAC TGGCATCTCG CCATTCAAGA AGCCATTTTG 
GAGAAGTGCA GTGATAATGA TGGAATCGTT CACATAGCTG TTGACAAAAA CTCAAGAGAG 
GGCTGCGTTT ACGTGAAGTG CCTTTCAGCA GAGCATTCTG GGAAAGCCTT CAAAGCGCTC 
CACGGCTCCT GGTTCGATGG TAAGCTGGTG ACGGTCAAGT ACCTGCGCCT GGATCGCTAC 
CACCAGCGTT TCCCACAAGC CCTAGGCTCC AACACACCTC TCAAACCCTC CAGCTCCAGC 
ATGAACAGCA TGTCTCGTCT GAACCAGCGC TCCAGCACGT CCAGCTTGAA GGACACGTTC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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lemd3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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