ash2l cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ash2l
Entrez Gene ID	558193
Full Name	ash2 (absent, small, or homeotic)-like (Drosophila)
Synonyms	fb34c06,fe38b04,fi20h02,wu:fb34c06,wu:fe38b04,wu:fi20h02,zgc:171444
General protein information	Preferred Names ash2 (absent, small, or homeotic)-like (Drosophila) Names set1/Ash2 histone methyltransferase complex subunit ASH2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005155525.3	XP_005155582.1	set1/Ash2 histone methyltransferase complex subunit ASH2 isoform X1
NM_001110105.2	NP_001103575.2	set1/Ash2 histone methyltransferase complex subunit ASH2
XM_005155525.4	XP_005155582.1	set1/Ash2 histone methyltransferase complex subunit ASH2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ASH2L	NP_001026566.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003705	XP_562582.3
Schizosaccharomyces pombe (fission yeast)	ash2	NP_596557.1
Mus musculus (house mouse)	Ash2l	NP_035921.2
Danio rerio (zebrafish)	ash2l	NP_001103575.2
Drosophila melanogaster (fruit fly)	ash2	NP_788735.1
Pan troglodytes (chimpanzee)	ASH2L	XP_001170172.1
	ASH2L	XP_002805359.1
Canis lupus familiaris (dog)	ASH2L	XP_005629916.1
Rattus norvegicus (Norway rat)	Ash2l	NP_001099559.1
Xenopus tropicalis (tropical clawed frog)	ash2l	XP_002932768.2
Homo sapiens (human)	ASH2L	NP_004665.2
Bos taurus (cattle)	ASH2L	NP_001192402.1
Caenorhabditis elegans (roundworm)	ash-2	NP_496553.1

Gene Ontology
Bibliography

Related articles in PubMed

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
Brocal I, White RJ, Dooley CM, Carruthers SN, Clark R, Hall A, Busch-Nentwich EM, Stemple DL, Kettleborough RN
BMC genomics17259(2016 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
Gagnon JA, Valen E, Thyme SB, Huang P, Akhmetova L, Ahkmetova L, Pauli A, Montague TG, Zimmerman S, Richter C, Schier AF
PloS one9(5)e98186(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ash2l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ash2l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35509	NM_001110105.2 Latest version!	Danio rerio ash2 like, histone lysine methyltransferase complex subunit (ash2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa15681	XM_005155525.4 Latest version!	Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa15681	XM_005155525.3	Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ash2l ( NM_001110105.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103575.2 Danio rerio ash2 like, histone lysine methyltransferase complex subunit (ash2l), mRNA.

Price & Availability↑

CloneID	ODa35509
Clone ID Related Accession (Same CDS sequence)	NM_001110105.2
Accession Version	NM_001110105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	set1/Ash2 histone methyltransferase complex subunit ASH2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT027796.13, CT698001.2, CT627023.2, CT679794.2 and CT679795.2. On Mar 11, 2009 this sequence version replaced NM_001110105.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01001676.1, GDQH01001239.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGATCA 
ACCTTTTCAG GTGGGCTTCC TTCAGCTGCT ACAGGAAAAG GCAGAGGAGC CAAACGCAAA 
CAGCAGCAGC AACAGGATGG TGCCGCCACT GGAGCAGCCA AGAGAACACG CAGTGACCCT 
CTGTTCTCAG CTCAGCGTCT GCCGCCTCAC GGTTATCCTC TTGAACATCC CTTCAACAAA 
GATGGCTATC GCTACATCCT AGCAGAGCCG GATCCTCATG CTCCCGACCC GGAAAAACTG 
GAGCTGGACT GCTGGGCTGG AAAGCCCATT CCTGGAGACC TGTACCGGGC ATGTCTGTAC 
GAGAGAGTCC TGCTAGCATT GCATGACAGA GCTCCGCAGC TGAAGATTTC TGATGACCGG 
CTGACGGTGA CCGGAGAGAA AGGCTACTCG ATGGTGCGAG CGTCTCACGG GGTCAGAAAA 
GGCTCGTGGT TTTTTGAGGT GACTGTGGAT GAGATGCCTC AGGACGCTGC AGCTCGACTG 
GGCTGGTCGC AGCCTTTAGG AAATCTGCAG GCTCCTTTGG GTTATGATAA GTTCAGCTAC 
TCGTGGCGCA GTAAGAAAGG CACACGCTTC CATCAGTCCA TTGGAAAGCA TTACTCTGAT 
GGCTACGGTC TGGGAGATAT CTTGGGCTTC TTCATCGAGC TTCCAGACGA GACTGAAACC 
GCCAAGGCCC TGCCAGACAC ATACAAGGAC AAAGCACTGA TCAAATTCAA AAGCTACTTA 
TACTTTGAAG AGAAAGATTA TGTGGACAAG GCTGAGAAGA ACTTGAAACC CACCAGTACC 
AGCAGAATGA TATTTTTCAA AAACGGCGTG AATCAAGGTG TGGCTTATGA AGATCTGTTT 
GAGGGCATGT ACTACCCCGC TATATCCCTC TACAAGGGCT GCACGGTCTC TGTCAATTTT 
GGACCCCACT TCAAATACCC ACCAAAAGAC ATCAAGTTTC AGCCAATGAG CGATATGGGT 
TGGGGTGCAG TCATCGAGCA CACACTGGCT GATATGCTGT ACCACGTGGA GACTGAAGTG 
GACGGCCGAC GAAGCCCTCA ATATGAAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103575.2
CDS	28..1800
Translation	MAAEARSVTERDTADGDASFGDLTTNMETESSSGKETMDTAGDSSEAADAQTGSGDDENGRQLGEVELQCALCMKWFTADTFSIDTATCLPFMTNYVFHCNVCHHSGNTYFLRKQANLKEMCLTALANLTWRSRTQEEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKLTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLGNIGPSYDNQKQMTTVTGAGGLNGGSTFSGGLPSAATGKGRGAKRKQQQQQDGAATGAAKRTRSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVTGEKGYSMVRASHGVRKGSWFFEVTVDEMPQDAAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTRFHQSIGKHYSDGYGLGDILGFFIELPDETETAKALPDTYKDKALIKFKSYLYFEEKDYVDKAEKNLKPTSTSRMIFFKNGVNQGVAYEDLFEGMYYPAISLYKGCTVSVNFGPHFKYPPKDIKFQPMSDMGWGAVIEHTLADMLYHVETEVDGRRSPQYEG

Target ORF information:

RefSeq Version	NM_001110105.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ash2 like, histone lysine methyltransferase complex subunit (ash2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110105.2

ORF Insert Sequence:

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGATCA 
ACCTTTTCAG GTGGGCTTCC TTCAGCTGCT ACAGGAAAAG GCAGAGGAGC CAAACGCAAA 
CAGCAGCAGC AACAGGATGG TGCCGCCACT GGAGCAGCCA AGAGAACACG CAGTGACCCT 
CTGTTCTCAG CTCAGCGTCT GCCGCCTCAC GGTTATCCTC TTGAACATCC CTTCAACAAA 
GATGGCTATC GCTACATCCT AGCAGAGCCG GATCCTCATG CTCCCGACCC GGAAAAACTG 
GAGCTGGACT GCTGGGCTGG AAAGCCCATT CCTGGAGACC TGTACCGGGC ATGTCTGTAC 
GAGAGAGTCC TGCTAGCATT GCATGACAGA GCTCCGCAGC TGAAGATTTC TGATGACCGG 
CTGACGGTGA CCGGAGAGAA AGGCTACTCG ATGGTGCGAG CGTCTCACGG GGTCAGAAAA 
GGCTCGTGGT TTTTTGAGGT GACTGTGGAT GAGATGCCTC AGGACGCTGC AGCTCGACTG 
GGCTGGTCGC AGCCTTTAGG AAATCTGCAG GCTCCTTTGG GTTATGATAA GTTCAGCTAC 
TCGTGGCGCA GTAAGAAAGG CACACGCTTC CATCAGTCCA TTGGAAAGCA TTACTCTGAT 
GGCTACGGTC TGGGAGATAT CTTGGGCTTC TTCATCGAGC TTCCAGACGA GACTGAAACC 
GCCAAGGCCC TGCCAGACAC ATACAAGGAC AAAGCACTGA TCAAATTCAA AAGCTACTTA 
TACTTTGAAG AGAAAGATTA TGTGGACAAG GCTGAGAAGA ACTTGAAACC CACCAGTACC 
AGCAGAATGA TATTTTTCAA AAACGGCGTG AATCAAGGTG TGGCTTATGA AGATCTGTTT 
GAGGGCATGT ACTACCCCGC TATATCCCTC TACAAGGGCT GCACGGTCTC TGTCAATTTT 
GGACCCCACT TCAAATACCC ACCAAAAGAC ATCAAGTTTC AGCCAATGAG CGATATGGGT 
TGGGGTGCAG TCATCGAGCA CACACTGGCT GATATGCTGT ACCACGTGGA GACTGAAGTG 
GACGGCCGAC GAAGCCCTCA ATATGAAGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash2l ( XM_005155525.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155582.1 Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15681
Clone ID Related Accession (Same CDS sequence)	XM_005155525.3 , XM_005155525.4
Accession Version	XM_005155525.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	set1/Ash2 histone methyltransferase complex subunit ASH2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155525.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGGCTT 
CCTTCAGCTG CTACAGGAAA AGGCAGAGGA GCCAAACGCA AACAGCAGCA GCAACAGGAT 
GGTGCCGCCA CTGGAGCAGC CAAGAGAACA CGCAGTGACC CTCTGTTCTC AGCTCAGCGT 
CTGCCGCCTC ACGGTTATCC TCTTGAACAT CCCTTCAACA AAGATGGCTA TCGCTACATC 
CTAGCAGAGC CGGATCCTCA TGCTCCCGAC CCGGAAAAAC TGGAGCTGGA CTGCTGGGCT 
GGAAAGCCCA TTCCTGGAGA CCTGTACCGG GCATGTCTGT ACGAGAGAGT CCTGCTAGCA 
TTGCATGACA GAGCTCCGCA GCTGAAGATT TCTGATGACC GGCTGACGGT GACCGGAGAG 
AAAGGCTACT CGATGGTGCG AGCGTCTCAC GGGGTCAGAA AAGGCTCGTG GTTTTTTGAG 
GTGACTGTGG ATGAGATGCC TCAGGACGCT GCAGCTCGAC TGGGCTGGTC GCAGCCTTTA 
GGAAATCTGC AGGCTCCTTT GGGTTATGAT AAGTTCAGCT ACTCGTGGCG CAGTAAGAAA 
GGCACACGCT TCCATCAGTC CATTGGAAAG CATTACTCTG ATGGCTACGG TCTGGGAGAT 
ATCTTGGGCT TCTTCATCGA GCTTCCAGAC GAGACTGAAA CCGCCAAGGC CCTGCCAGAC 
ACATACAAGG ACAAAGCACT GATCAAATTC AAAAGCTACT TATACTTTGA AGAGAAAGAT 
TATGTGGACA AGGCTGAGAA GAACTTGAAA CCCACCAGTA CCAGCAGAAT GATATTTTTC 
AAAAACGGCG TGAATCAAGG TGTGGCTTAT GAAGATCTGT TTGAGGGCAT GTACTACCCC 
GCTATATCCC TCTACAAGGG CTGCACGGTC TCTGTCAATT TTGGACCCCA CTTCAAATAC 
CCACCAAAAG ACATCAAGTT TCAGCCAATG AGCGATATGG GTTGGGGTGC AGTCATCGAG 
CACACACTGG CTGATATGCT GTACCACGTG GAGACTGAAG TGGACGGCCG ACGAAGCCCT 
CAATATGAAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155582.1
CDS	46..1800
Translation	MAAEARSVTERDTADGDASFGDLTTNMETESSSGKETMDTAGDSSEAADAQTGSGDDENGRQLGEVELQCALCMKWFTADTFSIDTATCLPFMTNYVFHCNVCHHSGNTYFLRKQANLKEMCLTALANLTWRSRTQEEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKLTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLGNIGPSYDNQKQMTTVTGAGGLNGGLPSAATGKGRGAKRKQQQQQDGAATGAAKRTRSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVTGEKGYSMVRASHGVRKGSWFFEVTVDEMPQDAAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTRFHQSIGKHYSDGYGLGDILGFFIELPDETETAKALPDTYKDKALIKFKSYLYFEEKDYVDKAEKNLKPTSTSRMIFFKNGVNQGVAYEDLFEGMYYPAISLYKGCTVSVNFGPHFKYPPKDIKFQPMSDMGWGAVIEHTLADMLYHVETEVDGRRSPQYEG

Target ORF information:

RefSeq Version	XM_005155525.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155525.3

ORF Insert Sequence:

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGGCTT 
CCTTCAGCTG CTACAGGAAA AGGCAGAGGA GCCAAACGCA AACAGCAGCA GCAACAGGAT 
GGTGCCGCCA CTGGAGCAGC CAAGAGAACA CGCAGTGACC CTCTGTTCTC AGCTCAGCGT 
CTGCCGCCTC ACGGTTATCC TCTTGAACAT CCCTTCAACA AAGATGGCTA TCGCTACATC 
CTAGCAGAGC CGGATCCTCA TGCTCCCGAC CCGGAAAAAC TGGAGCTGGA CTGCTGGGCT 
GGAAAGCCCA TTCCTGGAGA CCTGTACCGG GCATGTCTGT ACGAGAGAGT CCTGCTAGCA 
TTGCATGACA GAGCTCCGCA GCTGAAGATT TCTGATGACC GGCTGACGGT GACCGGAGAG 
AAAGGCTACT CGATGGTGCG AGCGTCTCAC GGGGTCAGAA AAGGCTCGTG GTTTTTTGAG 
GTGACTGTGG ATGAGATGCC TCAGGACGCT GCAGCTCGAC TGGGCTGGTC GCAGCCTTTA 
GGAAATCTGC AGGCTCCTTT GGGTTATGAT AAGTTCAGCT ACTCGTGGCG CAGTAAGAAA 
GGCACACGCT TCCATCAGTC CATTGGAAAG CATTACTCTG ATGGCTACGG TCTGGGAGAT 
ATCTTGGGCT TCTTCATCGA GCTTCCAGAC GAGACTGAAA CCGCCAAGGC CCTGCCAGAC 
ACATACAAGG ACAAAGCACT GATCAAATTC AAAAGCTACT TATACTTTGA AGAGAAAGAT 
TATGTGGACA AGGCTGAGAA GAACTTGAAA CCCACCAGTA CCAGCAGAAT GATATTTTTC 
AAAAACGGCG TGAATCAAGG TGTGGCTTAT GAAGATCTGT TTGAGGGCAT GTACTACCCC 
GCTATATCCC TCTACAAGGG CTGCACGGTC TCTGTCAATT TTGGACCCCA CTTCAAATAC 
CCACCAAAAG ACATCAAGTT TCAGCCAATG AGCGATATGG GTTGGGGTGC AGTCATCGAG 
CACACACTGG CTGATATGCT GTACCACGTG GAGACTGAAG TGGACGGCCG ACGAAGCCCT 
CAATATGAAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ash2l ( XM_005155525.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155582.1 Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15681
Clone ID Related Accession (Same CDS sequence)	XM_005155525.3 , XM_005155525.4
Accession Version	XM_005155525.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	set1/Ash2 histone methyltransferase complex subunit ASH2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155525.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGGCTT 
CCTTCAGCTG CTACAGGAAA AGGCAGAGGA GCCAAACGCA AACAGCAGCA GCAACAGGAT 
GGTGCCGCCA CTGGAGCAGC CAAGAGAACA CGCAGTGACC CTCTGTTCTC AGCTCAGCGT 
CTGCCGCCTC ACGGTTATCC TCTTGAACAT CCCTTCAACA AAGATGGCTA TCGCTACATC 
CTAGCAGAGC CGGATCCTCA TGCTCCCGAC CCGGAAAAAC TGGAGCTGGA CTGCTGGGCT 
GGAAAGCCCA TTCCTGGAGA CCTGTACCGG GCATGTCTGT ACGAGAGAGT CCTGCTAGCA 
TTGCATGACA GAGCTCCGCA GCTGAAGATT TCTGATGACC GGCTGACGGT GACCGGAGAG 
AAAGGCTACT CGATGGTGCG AGCGTCTCAC GGGGTCAGAA AAGGCTCGTG GTTTTTTGAG 
GTGACTGTGG ATGAGATGCC TCAGGACGCT GCAGCTCGAC TGGGCTGGTC GCAGCCTTTA 
GGAAATCTGC AGGCTCCTTT GGGTTATGAT AAGTTCAGCT ACTCGTGGCG CAGTAAGAAA 
GGCACACGCT TCCATCAGTC CATTGGAAAG CATTACTCTG ATGGCTACGG TCTGGGAGAT 
ATCTTGGGCT TCTTCATCGA GCTTCCAGAC GAGACTGAAA CCGCCAAGGC CCTGCCAGAC 
ACATACAAGG ACAAAGCACT GATCAAATTC AAAAGCTACT TATACTTTGA AGAGAAAGAT 
TATGTGGACA AGGCTGAGAA GAACTTGAAA CCCACCAGTA CCAGCAGAAT GATATTTTTC 
AAAAACGGCG TGAATCAAGG TGTGGCTTAT GAAGATCTGT TTGAGGGCAT GTACTACCCC 
GCTATATCCC TCTACAAGGG CTGCACGGTC TCTGTCAATT TTGGACCCCA CTTCAAATAC 
CCACCAAAAG ACATCAAGTT TCAGCCAATG AGCGATATGG GTTGGGGTGC AGTCATCGAG 
CACACACTGG CTGATATGCT GTACCACGTG GAGACTGAAG TGGACGGCCG ACGAAGCCCT 
CAATATGAAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155582.1
CDS	61..1815
Translation	MAAEARSVTERDTADGDASFGDLTTNMETESSSGKETMDTAGDSSEAADAQTGSGDDENGRQLGEVELQCALCMKWFTADTFSIDTATCLPFMTNYVFHCNVCHHSGNTYFLRKQANLKEMCLTALANLTWRSRTQEEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKLTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLGNIGPSYDNQKQMTTVTGAGGLNGGLPSAATGKGRGAKRKQQQQQDGAATGAAKRTRSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVTGEKGYSMVRASHGVRKGSWFFEVTVDEMPQDAAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTRFHQSIGKHYSDGYGLGDILGFFIELPDETETAKALPDTYKDKALIKFKSYLYFEEKDYVDKAEKNLKPTSTSRMIFFKNGVNQGVAYEDLFEGMYYPAISLYKGCTVSVNFGPHFKYPPKDIKFQPMSDMGWGAVIEHTLADMLYHVETEVDGRRSPQYEG

Target ORF information:

RefSeq Version	XM_005155525.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ash2 (absent, small, or homeotic)-like (Drosophila) (ash2l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155525.4

ORF Insert Sequence:

ATGGCTGCAG AGGCGCGATC TGTGACTGAG AGAGACACCG CGGACGGAGA TGCCTCTTTT 
GGAGATCTGA CCACAAACAT GGAGACCGAG TCGTCCAGTG GAAAGGAGAC TATGGATACA 
GCCGGGGACA GTTCTGAAGC AGCGGACGCT CAGACGGGCT CTGGAGACGA TGAGAACGGC 
CGTCAGCTGG GTGAAGTGGA GCTGCAGTGT GCGCTGTGCA TGAAGTGGTT CACCGCAGAC 
ACTTTCAGCA TCGACACGGC AACTTGCCTG CCATTCATGA CCAATTATGT GTTTCACTGC 
AACGTGTGCC ACCACAGTGG AAACACTTAC TTTCTGAGGA AACAAGCCAA TCTGAAGGAA 
ATGTGTCTCA CTGCTTTGGC AAATCTGACA TGGCGGTCCA GAACTCAAGA GGAGCATCCT 
AAAACTATGT TCTCTAAGGA TAAGGACATC ATACCTTTCA TTGACAAATA TTGGGAGTGT 
ATGACGACCC GCCAGAGACC CGGCAAACTA ACATGGCCAA ATAACATCGT CAAAACCATG 
AGCAAAGAGC GCGATGTTTT CCTCGTGAAA GAACATCCTG ATCCTGGCAG CAAAGACCCT 
GAAGAGGACT ATCCTAAATT TGGTTTGCTC GATCAGGATC TGGGCAACAT CGGACCGTCG 
TATGACAACC AGAAGCAGAT GACCACAGTC ACGGGTGCTG GAGGATTGAA CGGTGGGCTT 
CCTTCAGCTG CTACAGGAAA AGGCAGAGGA GCCAAACGCA AACAGCAGCA GCAACAGGAT 
GGTGCCGCCA CTGGAGCAGC CAAGAGAACA CGCAGTGACC CTCTGTTCTC AGCTCAGCGT 
CTGCCGCCTC ACGGTTATCC TCTTGAACAT CCCTTCAACA AAGATGGCTA TCGCTACATC 
CTAGCAGAGC CGGATCCTCA TGCTCCCGAC CCGGAAAAAC TGGAGCTGGA CTGCTGGGCT 
GGAAAGCCCA TTCCTGGAGA CCTGTACCGG GCATGTCTGT ACGAGAGAGT CCTGCTAGCA 
TTGCATGACA GAGCTCCGCA GCTGAAGATT TCTGATGACC GGCTGACGGT GACCGGAGAG 
AAAGGCTACT CGATGGTGCG AGCGTCTCAC GGGGTCAGAA AAGGCTCGTG GTTTTTTGAG 
GTGACTGTGG ATGAGATGCC TCAGGACGCT GCAGCTCGAC TGGGCTGGTC GCAGCCTTTA 
GGAAATCTGC AGGCTCCTTT GGGTTATGAT AAGTTCAGCT ACTCGTGGCG CAGTAAGAAA 
GGCACACGCT TCCATCAGTC CATTGGAAAG CATTACTCTG ATGGCTACGG TCTGGGAGAT 
ATCTTGGGCT TCTTCATCGA GCTTCCAGAC GAGACTGAAA CCGCCAAGGC CCTGCCAGAC 
ACATACAAGG ACAAAGCACT GATCAAATTC AAAAGCTACT TATACTTTGA AGAGAAAGAT 
TATGTGGACA AGGCTGAGAA GAACTTGAAA CCCACCAGTA CCAGCAGAAT GATATTTTTC 
AAAAACGGCG TGAATCAAGG TGTGGCTTAT GAAGATCTGT TTGAGGGCAT GTACTACCCC 
GCTATATCCC TCTACAAGGG CTGCACGGTC TCTGTCAATT TTGGACCCCA CTTCAAATAC 
CCACCAAAAG ACATCAAGTT TCAGCCAATG AGCGATATGG GTTGGGGTGC AGTCATCGAG 
CACACACTGG CTGATATGCT GTACCACGTG GAGACTGAAG TGGACGGCCG ACGAAGCCCT 
CAATATGAAG GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
BMC genomics17259(2016 Mar)
Brocal I,White RJ,Dooley CM,Carruthers SN,Clark R,Hall A,Busch-Nentwich EM,Stemple DL,Kettleborough RN

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.
PloS one9(5)e98186(2014)
Gagnon JA,Valen E,Thyme SB,Huang P,Akhmetova L,Ahkmetova L,Pauli A,Montague TG,Zimmerman S,Richter C,Schier AF

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オンライン注文

ash2l cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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