LOC558044 cDNA ORF clone, Danio rerio(zebrafish)

The following LOC558044 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC558044 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa04346 NM_001099989.1
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa10710 XM_005165320.4
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa10711 XM_005165321.4
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa10712 XM_005165322.3
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa10712 XM_021476188.1
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ODa42737 XM_021476187.1
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42737 XM_009301652.2
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa42738 XM_009301653.2
Latest version!
Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
ODa10711 XM_005165321.3 Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
ODa10710 XM_005165320.3 Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa04346
    Clone ID Related Accession (Same CDS sequence) NM_001099989.1
    Accession Version NM_001099989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7176bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-08-05
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, brain 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX276181.16 and CT025939.7. On Jul 26, 2007 this sequence version replaced XM_701863.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGACA TGGATTTTGA TCCACGTGAA
    CCTGAGCCAT GTCTCTCGCC AGCTGCCTTT GTAAACCAGG TGCAATATTC CAACATCCTG
    GAGGGCCGTT TCAAACAGCT GCAAGATGAG AGAGAGGCAG TGCAGAAGAA GACCTTCACT
    AAATGGGTGA ACTCTCACCT GGGTCGTGTG ACCTGCAGGA TCGGTGACCT CTACACTGAC
    CTCCGCGATG GACGCATGCT TATTCGACTG CTGGAGGTTC TCTCCGGGGA ACAACTGCCA
    AAGCCCACCA AAGGCCGGAT GCGCATCCAC TGTCTTGAGA ATGTTGATAA GGCTCTGCAG
    TTCCTGAAGG AGCAAAAAGT TCACCTGGAA AATATGGGTT CACACGACAT CGTTGATGGG
    AACCACCGTC TCACACTCGG ACTCATCTGG ACCATAATCC TCCGTTTCCA GATTCAAGAC
    ATTAGCGTTG AGACGGAGGA CAACAAGGAG AAGAAATCAG CCAAAGATGC TCTGCTGCTG
    TGGTGTCAGA TGAAGACTGC AGGGTACCCC AATGTCAATG TCCACAACTT CACCACCAGC
    TGGAGAGATG GTCTCGCGTT CAATGCCATA GTGCACAAAC ACAGGTCGGA CTTGATTGAC
    TTTGATAATC TCAAGCGCTC TAATGCCCAC TATAACCTGC AGAATGCCTT TAATGTGGCA
    GAGAAGGAGC TGGGTCTTAC CAAGCTGCTG GATCCAGAAG ATGTGAATGT AGATCAGCCT
    GATGAGAAGT CCATCATCAC ATATGTGGCA ACATACTACC ACTACTTCAG CAAGATGAAG
    GCACTCGCCG TTGAAGGAAA GAGGATTGGC AAGGTGCTGG ATTATGCTAT CGAGGCTGAT
    CAACTGATAG AGAAATATGA GACTCTGGCC TCAGAGCTGC TGCAGTGGAT TGAACAAACC
    ATCGTCACCC TAAATGATCG CCAGCTTGCT AACTCACTCA GTGCAGTTCA GAACCAGCTT
    CAAGCCTTCA ACACCTACCG CACTGTGGAG AAACCACCCA AGTTCACTGA GAAGGGCAAT
    TTGGAGGTTT TGCTCTTCAC CATTCAAAGT AAAATGAGAG CAAACAATCA GAAGGTATAC
    ATGCCAAGAG AGGGAAAACT TATCTCTGAC ATCAACAAGG CGTGGGAGAG GCTGGAGAAA
    GCAGAGCATG AGAGAGAGTT GGCACTGAGA AACGAACTGA TTCGTCAAGA GAAGCTGGAG
    ATGCTGGCTG CACGTTTTGA TCGCAAAGCA GCAATGAGAG AAACCTGGCT CAGTGAAAAC
    CAGCGGCTGG TGTCACAGGA CAATTTTGGT GTTGAACTGG GTGCTGTGGA GGCTGCCACT
    CGCAAACACG AGGCCATCGA GACGGACATC CAGGCATATG GTGAGCGTGT AGCCGCCGTA
    GAAGCTGTCG CTCGAGAACT GGAAGCAGAG GGATATCATG AAGTGCGACG TGTGCTGGCG
    CGGAGGGATA ATGTGCTCAG GCTGTGGGAG TATCTGAAGG AACTGCTTGC AGCACGACGA
    GAGAGGCTGA TGGCACACCG TGACCTGCAG CGCCTCCTAC AGGAATTGAG CTACATCATG
    GAATGGATGG AAGACATGAA GAGTCGTTTG CAATCTCAGG ACAGTGGAAA ACACCTTCAT
    GATGTGGAAG ACTTGCTCCA GAAGCACACA CTGGTGGAAG CTGATATATC TGCACAGGCT
    GAGAGAGTCA AAGCTGTTCA GGCCACTGCA AAGAGATTTG CTTCAAATGA ACAGAGTTAC
    AAGCCATGTG ACCCAGCACT GGTTGAAGAG AAAGTGGATC TTCTGGGTCG AGCGTATGGA
    GAACTGGGCC AGCTGGCTGC GGATCGACGA GTTCGTCTAG ATGACTCCCG AAGACTCTGG
    CAGTTCCTGT GGGAGCTGGG AGAAGAGGCC GCCTGGATCC GAGAGCAGGA GCAGATCATC
    TCTGGAGGAG ACTACGGTAA AGATCTGAGC TCCGCTCTTC ACCTCCTGTC TAAACATGAG
    GCTTTTCGGG ATGAGATGGC AGCTCGGTAT GGCCCTCTGG GGAACAGCAT TGCTGGTGGA
    GAAGCCCTGG TGAAGGAAGG CCACGTTGGA GCACCTGAGG TGACTGAGAG GATTAAAGAT
    GTGAGAGCAC AGTGGTCACA CCTAGAGGAG GCCTCACAGT TGCGGGAGCA GAGCCTGAAT
    GAGTCTGTTG CTTTCCATCA ATTCCAGACT GACGCCAATG ACATGGAGGC TTGGATACTG
    GAAACATTAA GACAGGTCTC CAGTCAAGAA GTGGGCCATG ATGAGTTTTC CACACAGACT
    CTGGCCAGAA AGCAGAGAGA GGTGGAAGAG GAGATTCAAA GTCACCGATC ACTCATAGAT
    TCATTACATG AACAGGGCTC ATTACTGCCT GAGGCATATG CACATTCCCC ACAGGTGGAG
    GGTCGTCTCC CAGCAATAGA GCAGCGATAT GAGGAGTTAG AGGGGTTGTC GTCGTCATGG
    CGACAGGCCT TGGATGGAGC GCTTGCGTTA TATCGCATGT TTAGTGAAGC TAGTGCCTGC
    CAACTGTGGG TTGGAGAAAA AGAGCAGTGG CTACACAACA TGGAGATTCC CACCAAACTG
    GAGGATCTGG AGGTGATCCA GCAGAGGTTT GATACTCTGG AGCCCGAGAT GAACACATTG
    GGTGGACGCA TTTCTGATGT CAATCAAGTT GCCCAACAGC TGCTTGGGTC AGACAACCGC
    AACAAAGACC AGATTGACCA GACACAAAAC CAACTCAATA ACAGGTGGTC TGATTTCCAG
    AGTCTGGCCA ACCAGCGTAA ACAAGCTCTA GAATCGGCTC TAAATATTCA GAACTATCAT
    CTGGAATGTA ATGAGATCAA GAGCTGGATG AAGGAGAAGA CCAAGGTCAT CGAGTCCACA
    CAGAGTCTTG GAAATGACCT GGCTGGAGTT ATGGCCCTAC AGCGCAAGCT CACTGGAATG
    GAGAGAGACC TGGAAGCCAT ACAGGGTAAG TTGGATGACC TGCGTTCGGA AGCCAAAAAG
    TTAGTATCTG AGCATCCTGA GCAGGAGGAA GAGATCAAGG GTCAGCTGGC TGAGATCCAG
    GAGGTGTGGG AGGAACTTCG TGCCACCATG AAACGGCGTG AGGAGTCTTT GGGTGAAGCT
    TCCAAGCTTC AGGGCTTTCT TCGAGATCTT GATGACTTCC AGGCCTGGCT GTCTCGTACA
    CAGACCACCG TGGCGTCCGA GGACACACCG ACCTCTCTGG CGGAAGCAGA GCGTCTGCTG
    GCTCAACATG AGGCCATAAA GAATGAAGTG GATAATTATA AAGAAGATTA TGAGAAGATG
    AGAGCTACTG GAGCTGAGGT AACTCAGGGT CAGACAGACG CCCAGCACAT GTTCTTAGCC
    CAGAGGCTGC AAGCGCTGGA CACCGGGTGG CATGAGCTGA GGAGAATGTG GGAGAGCCGC
    CACTGTGTCC TCGCTCAGGC CTTTGATTTC CAAACCCTGC TGCGAGATGC AAAACAGGCA
    GAGGGCTTCC TTAATAGCCA GGAGTATGTT CTGTCACATA CAGAGATGCC ATCCAGCCTG
    CAAGGGGCTG AGGAGGCCAT CAAGAAACAT GAGGACTTCC TCACTACAAT GGAGGCCAGT
    GAAGAAAAGA TCAACGGTGT GGTAGAGTCT GGACGGAGAC TTGTATCTGA TGGCAACACT
    TATGCGGACA AGATCCAGGA GAAGACTGAC TCTATTCAGG AAAGGCACCA AAAGAATAAA
    CAAGCTGCCA ATGAGCTGCT GGCTAAACTG AAGGATAACA GAGAGCTGCA GCATTTCCTG
    CAGGATGGAC AAGAGCTTAC TTTGTGGATA AATGAGAAGA TGCTGACTGC TCAGGATATG
    TCCTATGATG AGGCCAGAAA CCTCCACAGC AAGTGGCAGA AGCACCAGGC ATTCATGGCT
    GAGCTGGCCT CCAATAAAGA CTGGCTGGAC AAGATCGACA AGGAGGGTCA GGTGTTAGTA
    AAGGAGAAGC CCGAGTTGGA GCAAACTGTA TCAGAGACTC TGAGCGGTCT GCAGAAGCAG
    TGGGTAGAGC TTGAGAACAC CACCCAAGCC AAAGCTCAGT GTCTGTTTGA CGCTAACCGA
    GCGGAGCTGT TCACACAGAG CTGCTCAGCG CTGGACTCCT GGCTCCAGAA CATCTCCTCT
    CAACTCCAGA GCGACGACTT TGGAAAAGAT CTCACCAGCG TCAACATCCT GCTTAAAAAA
    CACCAGATGC TGGAGCATCA GATGGATGTG CGCGAGAAAG AAGTCCAGTC TCTGCAGTCA
    CAAGCTCTGG CCTTGGCCCA AGAAGATTCT GGGATAGTGG AAGTGGATGG GGAGCAGAGA
    AGAGTGACAG ACAGATTCTC TCAGCTGCAG GACCCTCTGA AAGAGCGGAG GCAGCATCTT
    CTGGCTTCAA AAGAGGCTCA TCAGTTCAAC AGAGATCTTG AGGATGAGAT TTTATGGGTG
    AAGGAGAGGA TGCCCCTTGC CACGTCTACA GACCATGGCA AAGATCTGCC CAGTGTTCAG
    CTGCTCATGA AAAAGAATCA GACTCTGCAG AAGGAGATTC AAGGTCACCA GCCCCGTATC
    GATGACATCC AGGCTCACGG TACGAATATG TCCCCTGGCA AAGAGTCTGA GATGGACAGG
    GAGAGGAGAG CTGCTCTTGA CGGCCGTCTG GCGGAGCTGA GAGAATTGTG GGCCCTTTTG
    ATTTCTGAGA CTGAGCAGAG GAACGTGAGG CTAGCTGAGG CTAACAGAGC GCAGCAGTTT
    TACACGGATG CCGCAGAGGC GGAGGCTTGG ATGGGAGAAC AAGAGCTTCA TATGATGTCA
    GAGGAGAAAG CGAAGGATGA ACAGAGTGCT CTAGTGATGG TGAAGAAACA CCAGATTCTG
    GAGCAGGCTC TTGAGGACTA CGCTCAGACC ATCCACCAGC TGGCCAACAG CAGCAGACTC
    ATGGTCAACA GCGAACACCC GGAGAGTGAA AGGATCACTC TGAGGCAGGC CCAGGTGGAT
    AAACTGTACG CAGGGTTGAA GGATCTAGCC GAGGAGAGGA GGGGTAAACT GCAGGAGAGA
    CTGAGATTGA CCCAGCTGAA GAGAGAGGTG GATGATTTAG AGCAGTGGAT CGCCGAAAGA
    GAGGTCGTCG CTGGATCTCA TGAACTTGGA CAAGACTACG AACATGTCAC GATGTTGCGT
    GACAAGTTCC GGGAGTTTGC GCGGGACACC AGCACAATTG GCCAGGAGCG TGTAGATGCA
    GTAAATGCTC AGGCAGATGA ACTTATCGAA TCCGGTCATC CTGAAAACGC CAGTGTGGCT
    GAGTGGAAGG ACGGACTCAA TGAGGCCTGG GCTGACCTGC TGGAACTCAT CGACACACGC
    ACACAAATGC TGGCCGCCTC GTATGAGCTG CATCGCTTCC ATCAAGACGC TCGGGAAGCT
    CTGGGGCTCA TACGAGAGAA GAAAGAGACG CTAGCGGGGG CTGAACTCGG ACGGGACTTG
    AATACTGTCC AGCACTTTCT CAGACAGCAT ACGGCCTATG AGCATGATGT GCAGGCACTC
    AGTGGGCAGG TCACACAGGT GCAAGATGAT GCTGCACGTC TGCAGAAAGC ATATGCTGGA
    GAGAAAGCGG ATGATATCCA TCGACATGAG CGTGCAGTCA CAGAGGCCTG GGAGGGACTT
    CAGTCTGCAA CTCAAACCAG GCGGCTGCTT CTATTGGACA CAGTGGAGAA GTTCAGATTC
    CTAAACATGG TTCGAGACCT CATGCTGTGG ATGGAGGGAA TCAACTTACA GATCCAGTCA
    CACGACAGTC CGAGGGACGT CTCCTCAGCT GGTCTGGTCA TCGCTAACCA TCAGGACATC
    AAGTCAGAGA TAGAGACTAG AGCAGACAGC TTTACTGCTT GCAGTGAAAT GGGACGCACA
    CTCATCAACA ACAATCACTA TGCTTCAGAT GAGATTCAAG AAAAATTGGA CCAGCTTCAG
    GCCAAACGCA CTGAAATTAA CCAGAAGTGG CAAGAAAAAA TGGACCACTT ACAGATTGTT
    CTGGAGGTTT TGCAGTTTGG CAGAGATGCA TCTATGGCCG AGTCGTGGCT GGCTGGGCAG
    GAGCCGCTGG TCAGGGCAGC AGAACTGGGC TCTAACGTAG ATGAAGTTGA GAGTCTCATC
    AAACGCCACG AAGCCTTTGA AAAACTGGCA GCAGGCTGGG AAGATCGATT CTCGCAGCTA
    GAGAAGCTCA CCACGCTGGA GGAGCAGGAA ATTCAAAGAC GAAGGGAAGA GGAAGAGAGG
    GCCCGACGGC CACCCACACC TCCCCCTGTA GAGGAAGTAG TACAGTCTGA GATTAATGAT
    TCTGCCGCAA GGACGAGTCT AGACCAGACT ACATTGAACC AGTCTGTATC TGTCAATGGT
    GTCTACAGTG ACCAGGACAC GTCACAGGGA TCAGAGTCTG AATCTGTTAA CGGTCCAGGC
    CGTGACAGTG GTCTTGACTC AGCATCACGG CAAGACCCTT CAGCAACACT GCCAGGGCGA
    GGAGGAGCAG AGGGGACCAC AGAAGCTATG GAGGGCATCC TATGCAGGAA ACAGGAAATG
    GAATCGCACA ACAAAAAATC AGCTAACAGA TCGTGGCAGA ATGTATATTG CGTTTTACGT
    AAAGGCAGTC TGGGCTTCTA TAAGGACAAC AAGAGTGCCT CAAATGGAAT CCCCTACCAT
    GGTGAAGTTC CCATCAGCCT CGGGGAGGCT GTCTGTGAGG TTGCCCATGA CTATAAGAAG
    AGGAAACACG TATTTAAACT TAGGCTCGGG GATGGAAAAG AGTACTTGTT CCAAGCGAAG
    GATGAGGCTG AGATGAGTTC ATGGATTCGA GCCATCCAGT CCTCCATGTC GTCCAGCGAG
    CGTTCGCCAG GTGCCTCCCG AGGTCTGAGT CGAGCAATGA CCATGCCGCC CATGTCTCCC
    AGCTCAGGCG AGGCCGGAGG AGTAACCATG CGCAACAAAG ACGGCAAAGA GAGAGACCGC
    GAGAAGCGCT TCAGTTTCTT CGGCAAGAAG AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093459.1
    CDS1..7176
    Translation

    Target ORF information:

    RefSeq Version NM_001099989.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001099989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGACA TGGATTTTGA TCCACGTGAA
    CCTGAGCCAT GTCTCTCGCC AGCTGCCTTT GTAAACCAGG TGCAATATTC CAACATCCTG
    GAGGGCCGTT TCAAACAGCT GCAAGATGAG AGAGAGGCAG TGCAGAAGAA GACCTTCACT
    AAATGGGTGA ACTCTCACCT GGGTCGTGTG ACCTGCAGGA TCGGTGACCT CTACACTGAC
    CTCCGCGATG GACGCATGCT TATTCGACTG CTGGAGGTTC TCTCCGGGGA ACAACTGCCA
    AAGCCCACCA AAGGCCGGAT GCGCATCCAC TGTCTTGAGA ATGTTGATAA GGCTCTGCAG
    TTCCTGAAGG AGCAAAAAGT TCACCTGGAA AATATGGGTT CACACGACAT CGTTGATGGG
    AACCACCGTC TCACACTCGG ACTCATCTGG ACCATAATCC TCCGTTTCCA GATTCAAGAC
    ATTAGCGTTG AGACGGAGGA CAACAAGGAG AAGAAATCAG CCAAAGATGC TCTGCTGCTG
    TGGTGTCAGA TGAAGACTGC AGGGTACCCC AATGTCAATG TCCACAACTT CACCACCAGC
    TGGAGAGATG GTCTCGCGTT CAATGCCATA GTGCACAAAC ACAGGTCGGA CTTGATTGAC
    TTTGATAATC TCAAGCGCTC TAATGCCCAC TATAACCTGC AGAATGCCTT TAATGTGGCA
    GAGAAGGAGC TGGGTCTTAC CAAGCTGCTG GATCCAGAAG ATGTGAATGT AGATCAGCCT
    GATGAGAAGT CCATCATCAC ATATGTGGCA ACATACTACC ACTACTTCAG CAAGATGAAG
    GCACTCGCCG TTGAAGGAAA GAGGATTGGC AAGGTGCTGG ATTATGCTAT CGAGGCTGAT
    CAACTGATAG AGAAATATGA GACTCTGGCC TCAGAGCTGC TGCAGTGGAT TGAACAAACC
    ATCGTCACCC TAAATGATCG CCAGCTTGCT AACTCACTCA GTGCAGTTCA GAACCAGCTT
    CAAGCCTTCA ACACCTACCG CACTGTGGAG AAACCACCCA AGTTCACTGA GAAGGGCAAT
    TTGGAGGTTT TGCTCTTCAC CATTCAAAGT AAAATGAGAG CAAACAATCA GAAGGTATAC
    ATGCCAAGAG AGGGAAAACT TATCTCTGAC ATCAACAAGG CGTGGGAGAG GCTGGAGAAA
    GCAGAGCATG AGAGAGAGTT GGCACTGAGA AACGAACTGA TTCGTCAAGA GAAGCTGGAG
    ATGCTGGCTG CACGTTTTGA TCGCAAAGCA GCAATGAGAG AAACCTGGCT CAGTGAAAAC
    CAGCGGCTGG TGTCACAGGA CAATTTTGGT GTTGAACTGG GTGCTGTGGA GGCTGCCACT
    CGCAAACACG AGGCCATCGA GACGGACATC CAGGCATATG GTGAGCGTGT AGCCGCCGTA
    GAAGCTGTCG CTCGAGAACT GGAAGCAGAG GGATATCATG AAGTGCGACG TGTGCTGGCG
    CGGAGGGATA ATGTGCTCAG GCTGTGGGAG TATCTGAAGG AACTGCTTGC AGCACGACGA
    GAGAGGCTGA TGGCACACCG TGACCTGCAG CGCCTCCTAC AGGAATTGAG CTACATCATG
    GAATGGATGG AAGACATGAA GAGTCGTTTG CAATCTCAGG ACAGTGGAAA ACACCTTCAT
    GATGTGGAAG ACTTGCTCCA GAAGCACACA CTGGTGGAAG CTGATATATC TGCACAGGCT
    GAGAGAGTCA AAGCTGTTCA GGCCACTGCA AAGAGATTTG CTTCAAATGA ACAGAGTTAC
    AAGCCATGTG ACCCAGCACT GGTTGAAGAG AAAGTGGATC TTCTGGGTCG AGCGTATGGA
    GAACTGGGCC AGCTGGCTGC GGATCGACGA GTTCGTCTAG ATGACTCCCG AAGACTCTGG
    CAGTTCCTGT GGGAGCTGGG AGAAGAGGCC GCCTGGATCC GAGAGCAGGA GCAGATCATC
    TCTGGAGGAG ACTACGGTAA AGATCTGAGC TCCGCTCTTC ACCTCCTGTC TAAACATGAG
    GCTTTTCGGG ATGAGATGGC AGCTCGGTAT GGCCCTCTGG GGAACAGCAT TGCTGGTGGA
    GAAGCCCTGG TGAAGGAAGG CCACGTTGGA GCACCTGAGG TGACTGAGAG GATTAAAGAT
    GTGAGAGCAC AGTGGTCACA CCTAGAGGAG GCCTCACAGT TGCGGGAGCA GAGCCTGAAT
    GAGTCTGTTG CTTTCCATCA ATTCCAGACT GACGCCAATG ACATGGAGGC TTGGATACTG
    GAAACATTAA GACAGGTCTC CAGTCAAGAA GTGGGCCATG ATGAGTTTTC CACACAGACT
    CTGGCCAGAA AGCAGAGAGA GGTGGAAGAG GAGATTCAAA GTCACCGATC ACTCATAGAT
    TCATTACATG AACAGGGCTC ATTACTGCCT GAGGCATATG CACATTCCCC ACAGGTGGAG
    GGTCGTCTCC CAGCAATAGA GCAGCGATAT GAGGAGTTAG AGGGGTTGTC GTCGTCATGG
    CGACAGGCCT TGGATGGAGC GCTTGCGTTA TATCGCATGT TTAGTGAAGC TAGTGCCTGC
    CAACTGTGGG TTGGAGAAAA AGAGCAGTGG CTACACAACA TGGAGATTCC CACCAAACTG
    GAGGATCTGG AGGTGATCCA GCAGAGGTTT GATACTCTGG AGCCCGAGAT GAACACATTG
    GGTGGACGCA TTTCTGATGT CAATCAAGTT GCCCAACAGC TGCTTGGGTC AGACAACCGC
    AACAAAGACC AGATTGACCA GACACAAAAC CAACTCAATA ACAGGTGGTC TGATTTCCAG
    AGTCTGGCCA ACCAGCGTAA ACAAGCTCTA GAATCGGCTC TAAATATTCA GAACTATCAT
    CTGGAATGTA ATGAGATCAA GAGCTGGATG AAGGAGAAGA CCAAGGTCAT CGAGTCCACA
    CAGAGTCTTG GAAATGACCT GGCTGGAGTT ATGGCCCTAC AGCGCAAGCT CACTGGAATG
    GAGAGAGACC TGGAAGCCAT ACAGGGTAAG TTGGATGACC TGCGTTCGGA AGCCAAAAAG
    TTAGTATCTG AGCATCCTGA GCAGGAGGAA GAGATCAAGG GTCAGCTGGC TGAGATCCAG
    GAGGTGTGGG AGGAACTTCG TGCCACCATG AAACGGCGTG AGGAGTCTTT GGGTGAAGCT
    TCCAAGCTTC AGGGCTTTCT TCGAGATCTT GATGACTTCC AGGCCTGGCT GTCTCGTACA
    CAGACCACCG TGGCGTCCGA GGACACACCG ACCTCTCTGG CGGAAGCAGA GCGTCTGCTG
    GCTCAACATG AGGCCATAAA GAATGAAGTG GATAATTATA AAGAAGATTA TGAGAAGATG
    AGAGCTACTG GAGCTGAGGT AACTCAGGGT CAGACAGACG CCCAGCACAT GTTCTTAGCC
    CAGAGGCTGC AAGCGCTGGA CACCGGGTGG CATGAGCTGA GGAGAATGTG GGAGAGCCGC
    CACTGTGTCC TCGCTCAGGC CTTTGATTTC CAAACCCTGC TGCGAGATGC AAAACAGGCA
    GAGGGCTTCC TTAATAGCCA GGAGTATGTT CTGTCACATA CAGAGATGCC ATCCAGCCTG
    CAAGGGGCTG AGGAGGCCAT CAAGAAACAT GAGGACTTCC TCACTACAAT GGAGGCCAGT
    GAAGAAAAGA TCAACGGTGT GGTAGAGTCT GGACGGAGAC TTGTATCTGA TGGCAACACT
    TATGCGGACA AGATCCAGGA GAAGACTGAC TCTATTCAGG AAAGGCACCA AAAGAATAAA
    CAAGCTGCCA ATGAGCTGCT GGCTAAACTG AAGGATAACA GAGAGCTGCA GCATTTCCTG
    CAGGATGGAC AAGAGCTTAC TTTGTGGATA AATGAGAAGA TGCTGACTGC TCAGGATATG
    TCCTATGATG AGGCCAGAAA CCTCCACAGC AAGTGGCAGA AGCACCAGGC ATTCATGGCT
    GAGCTGGCCT CCAATAAAGA CTGGCTGGAC AAGATCGACA AGGAGGGTCA GGTGTTAGTA
    AAGGAGAAGC CCGAGTTGGA GCAAACTGTA TCAGAGACTC TGAGCGGTCT GCAGAAGCAG
    TGGGTAGAGC TTGAGAACAC CACCCAAGCC AAAGCTCAGT GTCTGTTTGA CGCTAACCGA
    GCGGAGCTGT TCACACAGAG CTGCTCAGCG CTGGACTCCT GGCTCCAGAA CATCTCCTCT
    CAACTCCAGA GCGACGACTT TGGAAAAGAT CTCACCAGCG TCAACATCCT GCTTAAAAAA
    CACCAGATGC TGGAGCATCA GATGGATGTG CGCGAGAAAG AAGTCCAGTC TCTGCAGTCA
    CAAGCTCTGG CCTTGGCCCA AGAAGATTCT GGGATAGTGG AAGTGGATGG GGAGCAGAGA
    AGAGTGACAG ACAGATTCTC TCAGCTGCAG GACCCTCTGA AAGAGCGGAG GCAGCATCTT
    CTGGCTTCAA AAGAGGCTCA TCAGTTCAAC AGAGATCTTG AGGATGAGAT TTTATGGGTG
    AAGGAGAGGA TGCCCCTTGC CACGTCTACA GACCATGGCA AAGATCTGCC CAGTGTTCAG
    CTGCTCATGA AAAAGAATCA GACTCTGCAG AAGGAGATTC AAGGTCACCA GCCCCGTATC
    GATGACATCC AGGCTCACGG TACGAATATG TCCCCTGGCA AAGAGTCTGA GATGGACAGG
    GAGAGGAGAG CTGCTCTTGA CGGCCGTCTG GCGGAGCTGA GAGAATTGTG GGCCCTTTTG
    ATTTCTGAGA CTGAGCAGAG GAACGTGAGG CTAGCTGAGG CTAACAGAGC GCAGCAGTTT
    TACACGGATG CCGCAGAGGC GGAGGCTTGG ATGGGAGAAC AAGAGCTTCA TATGATGTCA
    GAGGAGAAAG CGAAGGATGA ACAGAGTGCT CTAGTGATGG TGAAGAAACA CCAGATTCTG
    GAGCAGGCTC TTGAGGACTA CGCTCAGACC ATCCACCAGC TGGCCAACAG CAGCAGACTC
    ATGGTCAACA GCGAACACCC GGAGAGTGAA AGGATCACTC TGAGGCAGGC CCAGGTGGAT
    AAACTGTACG CAGGGTTGAA GGATCTAGCC GAGGAGAGGA GGGGTAAACT GCAGGAGAGA
    CTGAGATTGA CCCAGCTGAA GAGAGAGGTG GATGATTTAG AGCAGTGGAT CGCCGAAAGA
    GAGGTCGTCG CTGGATCTCA TGAACTTGGA CAAGACTACG AACATGTCAC GATGTTGCGT
    GACAAGTTCC GGGAGTTTGC GCGGGACACC AGCACAATTG GCCAGGAGCG TGTAGATGCA
    GTAAATGCTC AGGCAGATGA ACTTATCGAA TCCGGTCATC CTGAAAACGC CAGTGTGGCT
    GAGTGGAAGG ACGGACTCAA TGAGGCCTGG GCTGACCTGC TGGAACTCAT CGACACACGC
    ACACAAATGC TGGCCGCCTC GTATGAGCTG CATCGCTTCC ATCAAGACGC TCGGGAAGCT
    CTGGGGCTCA TACGAGAGAA GAAAGAGACG CTAGCGGGGG CTGAACTCGG ACGGGACTTG
    AATACTGTCC AGCACTTTCT CAGACAGCAT ACGGCCTATG AGCATGATGT GCAGGCACTC
    AGTGGGCAGG TCACACAGGT GCAAGATGAT GCTGCACGTC TGCAGAAAGC ATATGCTGGA
    GAGAAAGCGG ATGATATCCA TCGACATGAG CGTGCAGTCA CAGAGGCCTG GGAGGGACTT
    CAGTCTGCAA CTCAAACCAG GCGGCTGCTT CTATTGGACA CAGTGGAGAA GTTCAGATTC
    CTAAACATGG TTCGAGACCT CATGCTGTGG ATGGAGGGAA TCAACTTACA GATCCAGTCA
    CACGACAGTC CGAGGGACGT CTCCTCAGCT GGTCTGGTCA TCGCTAACCA TCAGGACATC
    AAGTCAGAGA TAGAGACTAG AGCAGACAGC TTTACTGCTT GCAGTGAAAT GGGACGCACA
    CTCATCAACA ACAATCACTA TGCTTCAGAT GAGATTCAAG AAAAATTGGA CCAGCTTCAG
    GCCAAACGCA CTGAAATTAA CCAGAAGTGG CAAGAAAAAA TGGACCACTT ACAGATTGTT
    CTGGAGGTTT TGCAGTTTGG CAGAGATGCA TCTATGGCCG AGTCGTGGCT GGCTGGGCAG
    GAGCCGCTGG TCAGGGCAGC AGAACTGGGC TCTAACGTAG ATGAAGTTGA GAGTCTCATC
    AAACGCCACG AAGCCTTTGA AAAACTGGCA GCAGGCTGGG AAGATCGATT CTCGCAGCTA
    GAGAAGCTCA CCACGCTGGA GGAGCAGGAA ATTCAAAGAC GAAGGGAAGA GGAAGAGAGG
    GCCCGACGGC CACCCACACC TCCCCCTGTA GAGGAAGTAG TACAGTCTGA GATTAATGAT
    TCTGCCGCAA GGACGAGTCT AGACCAGACT ACATTGAACC AGTCTGTATC TGTCAATGGT
    GTCTACAGTG ACCAGGACAC GTCACAGGGA TCAGAGTCTG AATCTGTTAA CGGTCCAGGC
    CGTGACAGTG GTCTTGACTC AGCATCACGG CAAGACCCTT CAGCAACACT GCCAGGGCGA
    GGAGGAGCAG AGGGGACCAC AGAAGCTATG GAGGGCATCC TATGCAGGAA ACAGGAAATG
    GAATCGCACA ACAAAAAATC AGCTAACAGA TCGTGGCAGA ATGTATATTG CGTTTTACGT
    AAAGGCAGTC TGGGCTTCTA TAAGGACAAC AAGAGTGCCT CAAATGGAAT CCCCTACCAT
    GGTGAAGTTC CCATCAGCCT CGGGGAGGCT GTCTGTGAGG TTGCCCATGA CTATAAGAAG
    AGGAAACACG TATTTAAACT TAGGCTCGGG GATGGAAAAG AGTACTTGTT CCAAGCGAAG
    GATGAGGCTG AGATGAGTTC ATGGATTCGA GCCATCCAGT CCTCCATGTC GTCCAGCGAG
    CGTTCGCCAG GTGCCTCCCG AGGTCTGAGT CGAGCAATGA CCATGCCGCC CATGTCTCCC
    AGCTCAGGCG AGGCCGGAGG AGTAACCATG CGCAACAAAG ACGGCAAAGA GAGAGACCGC
    GAGAAGCGCT TCAGTTTCTT CGGCAAGAAG AAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10710
    Clone ID Related Accession (Same CDS sequence) XM_005165320.3 , XM_005165320.4
    Accession Version XM_005165320.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, brain 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TGGATCAGAG TCTGAATCTG TTAACGGTCC AGGCCGTGAC
    AGTGGTCTTG ACTCAGCATC ACGGCAAGAC CCTTCAGCAA CACTGCCAGG GCGAGGAGGA
    GCAGAGGGGA CCACAGAAGC TATGGAGGGC ATCCTATGCA GGAAACAGGA AATGGAATCG
    CACAACAAAA AATCAGCTAA CAGATCGTGG CAGAATGTAT ATTGCGTTTT ACGTAAAGGC
    AGTCTGGGCT TCTATAAGGA CAACAAGAGT GCCTCAAATG GAATCCCCTA CCATGGTGAA
    GTTCCCATCA GCCTCGGGGA GGCTGTCTGT GAGGTTGCCC ATGACTATAA GAAGAGGAAA
    CACGTATTTA AACTTAGGCT CGGGGATGGA AAAGAGTACT TGTTCCAAGC GAAGGATGAG
    GCTGAGATGA GTTCATGGAT TCGAGCCATC CAGTCCTCCA TGTCGTCCAG CGAGCGTTCG
    CCAGGTGCCT CCCGAGGTCT GAGTCGAGCA ATGACCATGC CGCCCATGTC TCCCAGCTCA
    GGCGAGGCCG GAGGAGTAAC CATGCGCAAC AAAGACGGCA AAGAGAGAGA CCGCGAGAAG
    CGCTTCAGTT TCTTCGGCAA GAAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165377.1
    CDS521..7690
    Translation

    Target ORF information:

    RefSeq Version XM_005165320.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165320.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TGGATCAGAG TCTGAATCTG TTAACGGTCC AGGCCGTGAC
    AGTGGTCTTG ACTCAGCATC ACGGCAAGAC CCTTCAGCAA CACTGCCAGG GCGAGGAGGA
    GCAGAGGGGA CCACAGAAGC TATGGAGGGC ATCCTATGCA GGAAACAGGA AATGGAATCG
    CACAACAAAA AATCAGCTAA CAGATCGTGG CAGAATGTAT ATTGCGTTTT ACGTAAAGGC
    AGTCTGGGCT TCTATAAGGA CAACAAGAGT GCCTCAAATG GAATCCCCTA CCATGGTGAA
    GTTCCCATCA GCCTCGGGGA GGCTGTCTGT GAGGTTGCCC ATGACTATAA GAAGAGGAAA
    CACGTATTTA AACTTAGGCT CGGGGATGGA AAAGAGTACT TGTTCCAAGC GAAGGATGAG
    GCTGAGATGA GTTCATGGAT TCGAGCCATC CAGTCCTCCA TGTCGTCCAG CGAGCGTTCG
    CCAGGTGCCT CCCGAGGTCT GAGTCGAGCA ATGACCATGC CGCCCATGTC TCCCAGCTCA
    GGCGAGGCCG GAGGAGTAAC CATGCGCAAC AAAGACGGCA AAGAGAGAGA CCGCGAGAAG
    CGCTTCAGTT TCTTCGGCAA GAAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10710
    Clone ID Related Accession (Same CDS sequence) XM_005165320.3 , XM_005165320.4
    Accession Version XM_005165320.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, brain 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165320.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TGGATCAGAG TCTGAATCTG TTAACGGTCC AGGCCGTGAC
    AGTGGTCTTG ACTCAGCATC ACGGCAAGAC CCTTCAGCAA CACTGCCAGG GCGAGGAGGA
    GCAGAGGGGA CCACAGAAGC TATGGAGGGC ATCCTATGCA GGAAACAGGA AATGGAATCG
    CACAACAAAA AATCAGCTAA CAGATCGTGG CAGAATGTAT ATTGCGTTTT ACGTAAAGGC
    AGTCTGGGCT TCTATAAGGA CAACAAGAGT GCCTCAAATG GAATCCCCTA CCATGGTGAA
    GTTCCCATCA GCCTCGGGGA GGCTGTCTGT GAGGTTGCCC ATGACTATAA GAAGAGGAAA
    CACGTATTTA AACTTAGGCT CGGGGATGGA AAAGAGTACT TGTTCCAAGC GAAGGATGAG
    GCTGAGATGA GTTCATGGAT TCGAGCCATC CAGTCCTCCA TGTCGTCCAG CGAGCGTTCG
    CCAGGTGCCT CCCGAGGTCT GAGTCGAGCA ATGACCATGC CGCCCATGTC TCCCAGCTCA
    GGCGAGGCCG GAGGAGTAAC CATGCGCAAC AAAGACGGCA AAGAGAGAGA CCGCGAGAAG
    CGCTTCAGTT TCTTCGGCAA GAAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165377.1
    CDS500..7669
    Translation

    Target ORF information:

    RefSeq Version XM_005165320.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165320.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TGGATCAGAG TCTGAATCTG TTAACGGTCC AGGCCGTGAC
    AGTGGTCTTG ACTCAGCATC ACGGCAAGAC CCTTCAGCAA CACTGCCAGG GCGAGGAGGA
    GCAGAGGGGA CCACAGAAGC TATGGAGGGC ATCCTATGCA GGAAACAGGA AATGGAATCG
    CACAACAAAA AATCAGCTAA CAGATCGTGG CAGAATGTAT ATTGCGTTTT ACGTAAAGGC
    AGTCTGGGCT TCTATAAGGA CAACAAGAGT GCCTCAAATG GAATCCCCTA CCATGGTGAA
    GTTCCCATCA GCCTCGGGGA GGCTGTCTGT GAGGTTGCCC ATGACTATAA GAAGAGGAAA
    CACGTATTTA AACTTAGGCT CGGGGATGGA AAAGAGTACT TGTTCCAAGC GAAGGATGAG
    GCTGAGATGA GTTCATGGAT TCGAGCCATC CAGTCCTCCA TGTCGTCCAG CGAGCGTTCG
    CCAGGTGCCT CCCGAGGTCT GAGTCGAGCA ATGACCATGC CGCCCATGTC TCCCAGCTCA
    GGCGAGGCCG GAGGAGTAAC CATGCGCAAC AAAGACGGCA AAGAGAGAGA CCGCGAGAAG
    CGCTTCAGTT TCTTCGGCAA GAAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10711
    Clone ID Related Accession (Same CDS sequence) XM_005165321.3 , XM_005165321.4
    Accession Version XM_005165321.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, brain 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGTC ATTATCGGTC
    TCTGTATCTG AACTCAAGAA AGCCGAACCT AAACCTCAGT CTAACCCAGT TTCTAAGCCT
    GTGGCTAAAC CCCACAAGGC ACAGGAGCGT GGATCAGAGT CTGAATCTGT TAACGGTCCA
    GGCCGTGACA GTGGTCTTGA CTCAGCATCA CGGCAAGACC CTTCAGCAAC ACTGCCAGGG
    CGAGGAGGAG CAGAGGGGAC CACAGAAGCT ATGGAGGGCA TCCTATGCAG GAAACAGGAA
    ATGGAATCGC ACAACAAAAA ATCAGCTAAC AGATCGTGGC AGAATGTATA TTGCGTTTTA
    CGTAAAGGCA GTCTGGGCTT CTATAAGGAC AACAAGAGTG CCTCAAATGG AATCCCCTAC
    CATGGTGAAG TTCCCATCAG CCTCGGGGAG GCTGTCTGTG AGGTTGCCCA TGACTATAAG
    AAGAGGAAAC ACGTATTTAA ACTTAGGCTC GGGGATGGAA AAGAGTACTT GTTCCAAGCG
    AAGGATGAGG CTGAGATGAG TTCATGGATT CGAGCCATCC AGTCCTCCAT GTCGTCCAGC
    GAGCGTTCGC CAGGTGCCTC CCGAGGTCTG AGTCGAGCAA TGACCATGCC GCCCATGTCT
    CCCAGCTCAG GCGAGGCCGG AGGAGTAACC ATGCGCAACA AAGACGGCAA AGAGAGAGAC
    CGCGAGAAGC GCTTCAGTTT CTTCGGCAAG AAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165378.1
    CDS588..7706
    Translation

    Target ORF information:

    RefSeq Version XM_005165321.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165321.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGTC ATTATCGGTC
    TCTGTATCTG AACTCAAGAA AGCCGAACCT AAACCTCAGT CTAACCCAGT TTCTAAGCCT
    GTGGCTAAAC CCCACAAGGC ACAGGAGCGT GGATCAGAGT CTGAATCTGT TAACGGTCCA
    GGCCGTGACA GTGGTCTTGA CTCAGCATCA CGGCAAGACC CTTCAGCAAC ACTGCCAGGG
    CGAGGAGGAG CAGAGGGGAC CACAGAAGCT ATGGAGGGCA TCCTATGCAG GAAACAGGAA
    ATGGAATCGC ACAACAAAAA ATCAGCTAAC AGATCGTGGC AGAATGTATA TTGCGTTTTA
    CGTAAAGGCA GTCTGGGCTT CTATAAGGAC AACAAGAGTG CCTCAAATGG AATCCCCTAC
    CATGGTGAAG TTCCCATCAG CCTCGGGGAG GCTGTCTGTG AGGTTGCCCA TGACTATAAG
    AAGAGGAAAC ACGTATTTAA ACTTAGGCTC GGGGATGGAA AAGAGTACTT GTTCCAAGCG
    AAGGATGAGG CTGAGATGAG TTCATGGATT CGAGCCATCC AGTCCTCCAT GTCGTCCAGC
    GAGCGTTCGC CAGGTGCCTC CCGAGGTCTG AGTCGAGCAA TGACCATGCC GCCCATGTCT
    CCCAGCTCAG GCGAGGCCGG AGGAGTAACC ATGCGCAACA AAGACGGCAA AGAGAGAGAC
    CGCGAGAAGC GCTTCAGTTT CTTCGGCAAG AAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10711
    Clone ID Related Accession (Same CDS sequence) XM_005165321.3 , XM_005165321.4
    Accession Version XM_005165321.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, brain 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGTC ATTATCGGTC
    TCTGTATCTG AACTCAAGAA AGCCGAACCT AAACCTCAGT CTAACCCAGT TTCTAAGCCT
    GTGGCTAAAC CCCACAAGGC ACAGGAGCGT GGATCAGAGT CTGAATCTGT TAACGGTCCA
    GGCCGTGACA GTGGTCTTGA CTCAGCATCA CGGCAAGACC CTTCAGCAAC ACTGCCAGGG
    CGAGGAGGAG CAGAGGGGAC CACAGAAGCT ATGGAGGGCA TCCTATGCAG GAAACAGGAA
    ATGGAATCGC ACAACAAAAA ATCAGCTAAC AGATCGTGGC AGAATGTATA TTGCGTTTTA
    CGTAAAGGCA GTCTGGGCTT CTATAAGGAC AACAAGAGTG CCTCAAATGG AATCCCCTAC
    CATGGTGAAG TTCCCATCAG CCTCGGGGAG GCTGTCTGTG AGGTTGCCCA TGACTATAAG
    AAGAGGAAAC ACGTATTTAA ACTTAGGCTC GGGGATGGAA AAGAGTACTT GTTCCAAGCG
    AAGGATGAGG CTGAGATGAG TTCATGGATT CGAGCCATCC AGTCCTCCAT GTCGTCCAGC
    GAGCGTTCGC CAGGTGCCTC CCGAGGTCTG AGTCGAGCAA TGACCATGCC GCCCATGTCT
    CCCAGCTCAG GCGAGGCCGG AGGAGTAACC ATGCGCAACA AAGACGGCAA AGAGAGAGAC
    CGCGAGAAGC GCTTCAGTTT CTTCGGCAAG AAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165378.1
    CDS549..7667
    Translation

    Target ORF information:

    RefSeq Version XM_005165321.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165321.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGTC ATTATCGGTC
    TCTGTATCTG AACTCAAGAA AGCCGAACCT AAACCTCAGT CTAACCCAGT TTCTAAGCCT
    GTGGCTAAAC CCCACAAGGC ACAGGAGCGT GGATCAGAGT CTGAATCTGT TAACGGTCCA
    GGCCGTGACA GTGGTCTTGA CTCAGCATCA CGGCAAGACC CTTCAGCAAC ACTGCCAGGG
    CGAGGAGGAG CAGAGGGGAC CACAGAAGCT ATGGAGGGCA TCCTATGCAG GAAACAGGAA
    ATGGAATCGC ACAACAAAAA ATCAGCTAAC AGATCGTGGC AGAATGTATA TTGCGTTTTA
    CGTAAAGGCA GTCTGGGCTT CTATAAGGAC AACAAGAGTG CCTCAAATGG AATCCCCTAC
    CATGGTGAAG TTCCCATCAG CCTCGGGGAG GCTGTCTGTG AGGTTGCCCA TGACTATAAG
    AAGAGGAAAC ACGTATTTAA ACTTAGGCTC GGGGATGGAA AAGAGTACTT GTTCCAAGCG
    AAGGATGAGG CTGAGATGAG TTCATGGATT CGAGCCATCC AGTCCTCCAT GTCGTCCAGC
    GAGCGTTCGC CAGGTGCCTC CCGAGGTCTG AGTCGAGCAA TGACCATGCC GCCCATGTCT
    CCCAGCTCAG GCGAGGCCGG AGGAGTAACC ATGCGCAACA AAGACGGCAA AGAGAGAGAC
    CGCGAGAAGC GCTTCAGTTT CTTCGGCAAG AAGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10712
    Clone ID Related Accession (Same CDS sequence) XM_005165322.3 , XM_021476188.1
    Accession Version XM_005165322.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, brain 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TTTTCATCGG ATTTTTCTAT ATGCCAAGGC AACTATTTTA
    TTTCCTAACA CTTGTATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165379.1
    CDS526..7146
    Translation

    Target ORF information:

    RefSeq Version XM_005165322.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005165322.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TTTTCATCGG ATTTTTCTAT ATGCCAAGGC AACTATTTTA
    TTTCCTAACA CTTGTATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa10712
    Clone ID Related Accession (Same CDS sequence) XM_005165322.3 , XM_021476188.1
    Accession Version XM_021476188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, brain 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TTTTCATCGG ATTTTTCTAT ATGCCAAGGC AACTATTTTA
    TTTCCTAACA CTTGTATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331863.1
    CDS1..6621
    Translation

    Target ORF information:

    RefSeq Version XM_021476188.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGT CATTATCGGT CTCTGTATCT
    GAACTCAAGA AAGCCGAACC TAAACCTCAG TCTAACCCAG TTTCTAAGCC TGTGGCTAAA
    CCCCACAAGG CACAGGAGCG TTTTCATCGG ATTTTTCTAT ATGCCAAGGC AACTATTTTA
    TTTCCTAACA CTTGTATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42737
    Clone ID Related Accession (Same CDS sequence) XM_009301652.2 , XM_021476187.1
    Accession Version XM_009301652.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, brain 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGG GATCAGAGTC TGAATCTGTT
    AACGGTCCAG GCCGTGACAG TGGTCTTGAC TCAGCATCAC GGCAAGACCC TTCAGCAACA
    CTGCCAGGGC GAGGAGGAGC AGAGGGGACC ACAGAAGCTA TGGAGGGCAT CCTATGCAGG
    AAACAGGAAA TGGAATCGCA CAACAAAAAA TCAGCTAACA GATCGTGGCA GAATGTATAT
    TGCGTTTTAC GTAAAGGCAG TCTGGGCTTC TATAAGGACA ACAAGAGTGC CTCAAATGGA
    ATCCCCTACC ATGGTGAAGT TCCCATCAGC CTCGGGGAGG CTGTCTGTGA GGTTGCCCAT
    GACTATAAGA AGAGGAAACA CGTATTTAAA CTTAGGCTCG GGGATGGAAA AGAGTACTTG
    TTCCAAGCGA AGGATGAGGC TGAGATGAGT TCATGGATTC GAGCCATCCA GTCCTCCATG
    TCGTCCAGCG AGCGTTCGCC AGGTGCCTCC CGAGGTCTGA GTCGAGCAAT GACCATGCCG
    CCCATGTCTC CCAGCTCAGG CGAGGCCGGA GGAGTAACCA TGCGCAACAA AGACGGCAAA
    GAGAGAGACC GCGAGAAGCG CTTCAGTTTC TTCGGCAAGA AGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299927.1
    CDS521..7588
    Translation

    Target ORF information:

    RefSeq Version XM_009301652.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301652.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGG GATCAGAGTC TGAATCTGTT
    AACGGTCCAG GCCGTGACAG TGGTCTTGAC TCAGCATCAC GGCAAGACCC TTCAGCAACA
    CTGCCAGGGC GAGGAGGAGC AGAGGGGACC ACAGAAGCTA TGGAGGGCAT CCTATGCAGG
    AAACAGGAAA TGGAATCGCA CAACAAAAAA TCAGCTAACA GATCGTGGCA GAATGTATAT
    TGCGTTTTAC GTAAAGGCAG TCTGGGCTTC TATAAGGACA ACAAGAGTGC CTCAAATGGA
    ATCCCCTACC ATGGTGAAGT TCCCATCAGC CTCGGGGAGG CTGTCTGTGA GGTTGCCCAT
    GACTATAAGA AGAGGAAACA CGTATTTAAA CTTAGGCTCG GGGATGGAAA AGAGTACTTG
    TTCCAAGCGA AGGATGAGGC TGAGATGAGT TCATGGATTC GAGCCATCCA GTCCTCCATG
    TCGTCCAGCG AGCGTTCGCC AGGTGCCTCC CGAGGTCTGA GTCGAGCAAT GACCATGCCG
    CCCATGTCTC CCAGCTCAGG CGAGGCCGGA GGAGTAACCA TGCGCAACAA AGACGGCAAA
    GAGAGAGACC GCGAGAAGCG CTTCAGTTTC TTCGGCAAGA AGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42737
    Clone ID Related Accession (Same CDS sequence) XM_009301652.2 , XM_021476187.1
    Accession Version XM_021476187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product spectrin beta chain, brain 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGG GATCAGAGTC TGAATCTGTT
    AACGGTCCAG GCCGTGACAG TGGTCTTGAC TCAGCATCAC GGCAAGACCC TTCAGCAACA
    CTGCCAGGGC GAGGAGGAGC AGAGGGGACC ACAGAAGCTA TGGAGGGCAT CCTATGCAGG
    AAACAGGAAA TGGAATCGCA CAACAAAAAA TCAGCTAACA GATCGTGGCA GAATGTATAT
    TGCGTTTTAC GTAAAGGCAG TCTGGGCTTC TATAAGGACA ACAAGAGTGC CTCAAATGGA
    ATCCCCTACC ATGGTGAAGT TCCCATCAGC CTCGGGGAGG CTGTCTGTGA GGTTGCCCAT
    GACTATAAGA AGAGGAAACA CGTATTTAAA CTTAGGCTCG GGGATGGAAA AGAGTACTTG
    TTCCAAGCGA AGGATGAGGC TGAGATGAGT TCATGGATTC GAGCCATCCA GTCCTCCATG
    TCGTCCAGCG AGCGTTCGCC AGGTGCCTCC CGAGGTCTGA GTCGAGCAAT GACCATGCCG
    CCCATGTCTC CCAGCTCAGG CGAGGCCGGA GGAGTAACCA TGCGCAACAA AGACGGCAAA
    GAGAGAGACC GCGAGAAGCG CTTCAGTTTC TTCGGCAAGA AGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331862.1
    CDS1..7068
    Translation

    Target ORF information:

    RefSeq Version XM_021476187.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGAGTACCA TCTCACCGAC TGATTTCGAC AGCGTGGAGA TCCAGCAGCA GTACAACGAC 
    ATCAACAACC GCTGGGACAT TGCAGCCGAA ACAGAATGGG ACAACGAGAA CAGTTCTGCA
    CGTTTGTTTG AGCGTTCTCG AATCAAAGCA CTCGCAGATG AGAGAGAGGC AGTGCAGAAG
    AAGACCTTCA CTAAATGGGT GAACTCTCAC CTGGGTCGTG TGACCTGCAG GATCGGTGAC
    CTCTACACTG ACCTCCGCGA TGGACGCATG CTTATTCGAC TGCTGGAGGT TCTCTCCGGG
    GAACAACTGC CAAAGCCCAC CAAAGGCCGG ATGCGCATCC ACTGTCTTGA GAATGTTGAT
    AAGGCTCTGC AGTTCCTGAA GGAGCAAAAA GTTCACCTGG AAAATATGGG TTCACACGAC
    ATCGTTGATG GGAACCACCG TCTCACACTC GGACTCATCT GGACCATAAT CCTCCGTTTC
    CAGATTCAAG ACATTAGCGT TGAGACGGAG GACAACAAGG AGAAGAAATC AGCCAAAGAT
    GCTCTGCTGC TGTGGTGTCA GATGAAGACT GCAGGGTACC CCAATGTCAA TGTCCACAAC
    TTCACCACCA GCTGGAGAGA TGGTCTCGCG TTCAATGCCA TAGTGCACAA ACACAGGTCG
    GACTTGATTG ACTTTGATAA TCTCAAGCGC TCTAATGCCC ACTATAACCT GCAGAATGCC
    TTTAATGTGG CAGAGAAGGA GCTGGGTCTT ACCAAGCTGC TGGATCCAGA AGATGTGAAT
    GTAGATCAGC CTGATGAGAA GTCCATCATC ACATATGTGG CAACATACTA CCACTACTTC
    AGCAAGATGA AGGCACTCGC CGTTGAAGGA AAGAGGATTG GCAAGGTGCT GGATTATGCT
    ATCGAGGCTG ATCAACTGAT AGAGAAATAT GAGACTCTGG CCTCAGAGCT GCTGCAGTGG
    ATTGAACAAA CCATCGTCAC CCTAAATGAT CGCCAGCTTG CTAACTCACT CAGTGCAGTT
    CAGAACCAGC TTCAAGCCTT CAACACCTAC CGCACTGTGG AGAAACCACC CAAGTTCACT
    GAGAAGGGCA ATTTGGAGGT TTTGCTCTTC ACCATTCAAA GTAAAATGAG AGCAAACAAT
    CAGAAGGTAT ACATGCCAAG AGAGGGAAAA CTTATCTCTG ACATCAACAA GGCGTGGGAG
    AGGCTGGAGA AAGCAGAGCA TGAGAGAGAG TTGGCACTGA GAAACGAACT GATTCGTCAA
    GAGAAGCTGG AGATGCTGGC TGCACGTTTT GATCGCAAAG CAGCAATGAG AGAAACCTGG
    CTCAGTGAAA ACCAGCGGCT GGTGTCACAG GACAATTTTG GTGTTGAACT GGGTGCTGTG
    GAGGCTGCCA CTCGCAAACA CGAGGCCATC GAGACGGACA TCCAGGCATA TGGTGAGCGT
    GTAGCCGCCG TAGAAGCTGT CGCTCGAGAA CTGGAAGCAG AGGGATATCA TGAAGTGCGA
    CGTGTGCTGG CGCGGAGGGA TAATGTGCTC AGGCTGTGGG AGTATCTGAA GGAACTGCTT
    GCAGCACGAC GAGAGAGGCT GATGGCACAC CGTGACCTGC AGCGCCTCCT ACAGGAATTG
    AGCTACATCA TGGAATGGAT GGAAGACATG AAGAGTCGTT TGCAATCTCA GGACAGTGGA
    AAACACCTTC ATGATGTGGA AGACTTGCTC CAGAAGCACA CACTGGTGGA AGCTGATATA
    TCTGCACAGG CTGAGAGAGT CAAAGCTGTT CAGGCCACTG CAAAGAGATT TGCTTCAAAT
    GAACAGAGTT ACAAGCCATG TGACCCAGCA CTGGTTGAAG AGAAAGTGGA TCTTCTGGGT
    CGAGCGTATG GAGAACTGGG CCAGCTGGCT GCGGATCGAC GAGTTCGTCT AGATGACTCC
    CGAAGACTCT GGCAGTTCCT GTGGGAGCTG GGAGAAGAGG CCGCCTGGAT CCGAGAGCAG
    GAGCAGATCA TCTCTGGAGG AGACTACGGT AAAGATCTGA GCTCCGCTCT TCACCTCCTG
    TCTAAACATG AGGCTTTTCG GGATGAGATG GCAGCTCGGT ATGGCCCTCT GGGGAACAGC
    ATTGCTGGTG GAGAAGCCCT GGTGAAGGAA GGCCACGTTG GAGCACCTGA GGTGACTGAG
    AGGATTAAAG ATGTGAGAGC ACAGTGGTCA CACCTAGAGG AGGCCTCACA GTTGCGGGAG
    CAGAGCCTGA ATGAGTCTGT TGCTTTCCAT CAATTCCAGA CTGACGCCAA TGACATGGAG
    GCTTGGATAC TGGAAACATT AAGACAGGTC TCCAGTCAAG AAGTGGGCCA TGATGAGTTT
    TCCACACAGA CTCTGGCCAG AAAGCAGAGA GAGGTGGAAG AGGAGATTCA AAGTCACCGA
    TCACTCATAG ATTCATTACA TGAACAGGGC TCATTACTGC CTGAGGCATA TGCACATTCC
    CCACAGGTGG AGGGTCGTCT CCCAGCAATA GAGCAGCGAT ATGAGGAGTT AGAGGGGTTG
    TCGTCGTCAT GGCGACAGGC CTTGGATGGA GCGCTTGCGT TATATCGCAT GTTTAGTGAA
    GCTAGTGCCT GCCAACTGTG GGTTGGAGAA AAAGAGCAGT GGCTACACAA CATGGAGATT
    CCCACCAAAC TGGAGGATCT GGAGGTGATC CAGCAGAGGT TTGATACTCT GGAGCCCGAG
    ATGAACACAT TGGGTGGACG CATTTCTGAT GTCAATCAAG TTGCCCAACA GCTGCTTGGG
    TCAGACAACC GCAACAAAGA CCAGATTGAC CAGACACAAA ACCAACTCAA TAACAGGTGG
    TCTGATTTCC AGAGTCTGGC CAACCAGCGT AAACAAGCTC TAGAATCGGC TCTAAATATT
    CAGAACTATC ATCTGGAATG TAATGAGATC AAGAGCTGGA TGAAGGAGAA GACCAAGGTC
    ATCGAGTCCA CACAGAGTCT TGGAAATGAC CTGGCTGGAG TTATGGCCCT ACAGCGCAAG
    CTCACTGGAA TGGAGAGAGA CCTGGAAGCC ATACAGGGTA AGTTGGATGA CCTGCGTTCG
    GAAGCCAAAA AGTTAGTATC TGAGCATCCT GAGCAGGAGG AAGAGATCAA GGGTCAGCTG
    GCTGAGATCC AGGAGGTGTG GGAGGAACTT CGTGCCACCA TGAAACGGCG TGAGGAGTCT
    TTGGGTGAAG CTTCCAAGCT TCAGGGCTTT CTTCGAGATC TTGATGACTT CCAGGCCTGG
    CTGTCTCGTA CACAGACCAC CGTGGCGTCC GAGGACACAC CGACCTCTCT GGCGGAAGCA
    GAGCGTCTGC TGGCTCAACA TGAGGCCATA AAGAATGAAG TGGATAATTA TAAAGAAGAT
    TATGAGAAGA TGAGAGCTAC TGGAGCTGAG GTAACTCAGG GTCAGACAGA CGCCCAGCAC
    ATGTTCTTAG CCCAGAGGCT GCAAGCGCTG GACACCGGGT GGCATGAGCT GAGGAGAATG
    TGGGAGAGCC GCCACTGTGT CCTCGCTCAG GCCTTTGATT TCCAAACCCT GCTGCGAGAT
    GCAAAACAGG CAGAGGGCTT CCTTAATAGC CAGGAGTATG TTCTGTCACA TACAGAGATG
    CCATCCAGCC TGCAAGGGGC TGAGGAGGCC ATCAAGAAAC ATGAGGACTT CCTCACTACA
    ATGGAGGCCA GTGAAGAAAA GATCAACGGT GTGGTAGAGT CTGGACGGAG ACTTGTATCT
    GATGGCAACA CTTATGCGGA CAAGATCCAG GAGAAGACTG ACTCTATTCA GGAAAGGCAC
    CAAAAGAATA AACAAGCTGC CAATGAGCTG CTGGCTAAAC TGAAGGATAA CAGAGAGCTG
    CAGCATTTCC TGCAGGATGG ACAAGAGCTT ACTTTGTGGA TAAATGAGAA GATGCTGACT
    GCTCAGGATA TGTCCTATGA TGAGGCCAGA AACCTCCACA GCAAGTGGCA GAAGCACCAG
    GCATTCATGG CTGAGCTGGC CTCCAATAAA GACTGGCTGG ACAAGATCGA CAAGGAGGGT
    CAGGTGTTAG TAAAGGAGAA GCCCGAGTTG GAGCAAACTG TATCAGAGAC TCTGAGCGGT
    CTGCAGAAGC AGTGGGTAGA GCTTGAGAAC ACCACCCAAG CCAAAGCTCA GTGTCTGTTT
    GACGCTAACC GAGCGGAGCT GTTCACACAG AGCTGCTCAG CGCTGGACTC CTGGCTCCAG
    AACATCTCCT CTCAACTCCA GAGCGACGAC TTTGGAAAAG ATCTCACCAG CGTCAACATC
    CTGCTTAAAA AACACCAGAT GCTGGAGCAT CAGATGGATG TGCGCGAGAA AGAAGTCCAG
    TCTCTGCAGT CACAAGCTCT GGCCTTGGCC CAAGAAGATT CTGGGATAGT GGAAGTGGAT
    GGGGAGCAGA GAAGAGTGAC AGACAGATTC TCTCAGCTGC AGGACCCTCT GAAAGAGCGG
    AGGCAGCATC TTCTGGCTTC AAAAGAGGCT CATCAGTTCA ACAGAGATCT TGAGGATGAG
    ATTTTATGGG TGAAGGAGAG GATGCCCCTT GCCACGTCTA CAGACCATGG CAAAGATCTG
    CCCAGTGTTC AGCTGCTCAT GAAAAAGAAT CAGACTCTGC AGAAGGAGAT TCAAGGTCAC
    CAGCCCCGTA TCGATGACAT CCAGGCTCAC GGTACGAATA TGTCCCCTGG CAAAGAGTCT
    GAGATGGACA GGGAGAGGAG AGCTGCTCTT GACGGCCGTC TGGCGGAGCT GAGAGAATTG
    TGGGCCCTTT TGATTTCTGA GACTGAGCAG AGGAACGTGA GGCTAGCTGA GGCTAACAGA
    GCGCAGCAGT TTTACACGGA TGCCGCAGAG GCGGAGGCTT GGATGGGAGA ACAAGAGCTT
    CATATGATGT CAGAGGAGAA AGCGAAGGAT GAACAGAGTG CTCTAGTGAT GGTGAAGAAA
    CACCAGATTC TGGAGCAGGC TCTTGAGGAC TACGCTCAGA CCATCCACCA GCTGGCCAAC
    AGCAGCAGAC TCATGGTCAA CAGCGAACAC CCGGAGAGTG AAAGGATCAC TCTGAGGCAG
    GCCCAGGTGG ATAAACTGTA CGCAGGGTTG AAGGATCTAG CCGAGGAGAG GAGGGGTAAA
    CTGCAGGAGA GACTGAGATT GACCCAGCTG AAGAGAGAGG TGGATGATTT AGAGCAGTGG
    ATCGCCGAAA GAGAGGTCGT CGCTGGATCT CATGAACTTG GACAAGACTA CGAACATGTC
    ACGATGTTGC GTGACAAGTT CCGGGAGTTT GCGCGGGACA CCAGCACAAT TGGCCAGGAG
    CGTGTAGATG CAGTAAATGC TCAGGCAGAT GAACTTATCG AATCCGGTCA TCCTGAAAAC
    GCCAGTGTGG CTGAGTGGAA GGACGGACTC AATGAGGCCT GGGCTGACCT GCTGGAACTC
    ATCGACACAC GCACACAAAT GCTGGCCGCC TCGTATGAGC TGCATCGCTT CCATCAAGAC
    GCTCGGGAAG CTCTGGGGCT CATACGAGAG AAGAAAGAGA CGCTAGCGGG GGCTGAACTC
    GGACGGGACT TGAATACTGT CCAGCACTTT CTCAGACAGC ATACGGCCTA TGAGCATGAT
    GTGCAGGCAC TCAGTGGGCA GGTCACACAG GTGCAAGATG ATGCTGCACG TCTGCAGAAA
    GCATATGCTG GAGAGAAAGC GGATGATATC CATCGACATG AGCGTGCAGT CACAGAGGCC
    TGGGAGGGAC TTCAGTCTGC AACTCAAACC AGGCGGCTGC TTCTATTGGA CACAGTGGAG
    AAGTTCAGAT TCCTAAACAT GGTTCGAGAC CTCATGCTGT GGATGGAGGG AATCAACTTA
    CAGATCCAGT CACACGACAG TCCGAGGGAC GTCTCCTCAG CTGGTCTGGT CATCGCTAAC
    CATCAGGACA TCAAGTCAGA GATAGAGACT AGAGCAGACA GCTTTACTGC TTGCAGTGAA
    ATGGGACGCA CACTCATCAA CAACAATCAC TATGCTTCAG ATGAGATTCA AGAAAAATTG
    GACCAGCTTC AGGCCAAACG CACTGAAATT AACCAGAAGT GGCAAGAAAA AATGGACCAC
    TTACAGATTG TTCTGGAGGT TTTGCAGTTT GGCAGAGATG CATCTATGGC CGAGTCGTGG
    CTGGCTGGGC AGGAGCCGCT GGTCAGGGCA GCAGAACTGG GCTCTAACGT AGATGAAGTT
    GAGAGTCTCA TCAAACGCCA CGAAGCCTTT GAAAAACTGG CAGCAGGCTG GGAAGATCGA
    TTCTCGCAGC TAGAGAAGCT CACCACGCTG GAGGAGCAGG AAATTCAAAG ACGAAGGGAA
    GAGGAAGAGA GGGCCCGACG GCCACCCACA CCTCCCCCTG TAGAGGAAGT AGTACAGTCT
    GAGATTAATG ATTCTGCCGC AAGGACGAGT CTAGACCAGA CTACATTGAA CCAGTCTGTA
    TCTGTCAATG GTGTCTACAG TGACCAGGAC ACGTCACAGG GATCAGAGTC TGAATCTGTT
    AACGGTCCAG GCCGTGACAG TGGTCTTGAC TCAGCATCAC GGCAAGACCC TTCAGCAACA
    CTGCCAGGGC GAGGAGGAGC AGAGGGGACC ACAGAAGCTA TGGAGGGCAT CCTATGCAGG
    AAACAGGAAA TGGAATCGCA CAACAAAAAA TCAGCTAACA GATCGTGGCA GAATGTATAT
    TGCGTTTTAC GTAAAGGCAG TCTGGGCTTC TATAAGGACA ACAAGAGTGC CTCAAATGGA
    ATCCCCTACC ATGGTGAAGT TCCCATCAGC CTCGGGGAGG CTGTCTGTGA GGTTGCCCAT
    GACTATAAGA AGAGGAAACA CGTATTTAAA CTTAGGCTCG GGGATGGAAA AGAGTACTTG
    TTCCAAGCGA AGGATGAGGC TGAGATGAGT TCATGGATTC GAGCCATCCA GTCCTCCATG
    TCGTCCAGCG AGCGTTCGCC AGGTGCCTCC CGAGGTCTGA GTCGAGCAAT GACCATGCCG
    CCCATGTCTC CCAGCTCAGG CGAGGCCGGA GGAGTAACCA TGCGCAACAA AGACGGCAAA
    GAGAGAGACC GCGAGAAGCG CTTCAGTTTC TTCGGCAAGA AGAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42738
    Clone ID Related Accession (Same CDS sequence) XM_009301653.2
    Accession Version XM_009301653.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product spectrin beta chain, brain 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGGG ATCAGAGTCT
    GAATCTGTTA ACGGTCCAGG CCGTGACAGT GGTCTTGACT CAGCATCACG GCAAGACCCT
    TCAGCAACAC TGCCAGGGCG AGGAGGAGCA GAGGGGACCA CAGAAGCTAT GGAGGGCATC
    CTATGCAGGA AACAGGAAAT GGAATCGCAC AACAAAAAAT CAGCTAACAG ATCGTGGCAG
    AATGTATATT GCGTTTTACG TAAAGGCAGT CTGGGCTTCT ATAAGGACAA CAAGAGTGCC
    TCAAATGGAA TCCCCTACCA TGGTGAAGTT CCCATCAGCC TCGGGGAGGC TGTCTGTGAG
    GTTGCCCATG ACTATAAGAA GAGGAAACAC GTATTTAAAC TTAGGCTCGG GGATGGAAAA
    GAGTACTTGT TCCAAGCGAA GGATGAGGCT GAGATGAGTT CATGGATTCG AGCCATCCAG
    TCCTCCATGT CGTCCAGCGA GCGTTCGCCA GGTGCCTCCC GAGGTCTGAG TCGAGCAATG
    ACCATGCCGC CCATGTCTCC CAGCTCAGGC GAGGCCGGAG GAGTAACCAT GCGCAACAAA
    GACGGCAAAG AGAGAGACCG CGAGAAGCGC TTCAGTTTCT TCGGCAAGAA GAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299928.1
    CDS588..7604
    Translation

    Target ORF information:

    RefSeq Version XM_009301653.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio spectrin, beta, non-erythrocytic 1 (LOC558044), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301653.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGATTTTG ATCCACGTGA ACCTGAGCCA TGTCTCTCGC CAGCTGCCTT TGTAAACCAG 
    GTGCAATATT CCAACATCCT GGAGGGCCGT TTCAAACAGC TGCAAGATGA GAGAGAGGCA
    GTGCAGAAGA AGACCTTCAC TAAATGGGTG AACTCTCACC TGGGTCGTGT GACCTGCAGG
    ATCGGTGACC TCTACACTGA CCTCCGCGAT GGACGCATGC TTATTCGACT GCTGGAGGTT
    CTCTCCGGGG AACAACTGCC AAAGCCCACC AAAGGCCGGA TGCGCATCCA CTGTCTTGAG
    AATGTTGATA AGGCTCTGCA GTTCCTGAAG GAGCAAAAAG TTCACCTGGA AAATATGGGT
    TCACACGACA TCGTTGATGG GAACCACCGT CTCACACTCG GACTCATCTG GACCATAATC
    CTCCGTTTCC AGATTCAAGA CATTAGCGTT GAGACGGAGG ACAACAAGGA GAAGAAATCA
    GCCAAAGATG CTCTGCTGCT GTGGTGTCAG ATGAAGACTG CAGGGTACCC CAATGTCAAT
    GTCCACAACT TCACCACCAG CTGGAGAGAT GGTCTCGCGT TCAATGCCAT AGTGCACAAA
    CACAGGTCGG ACTTGATTGA CTTTGATAAT CTCAAGCGCT CTAATGCCCA CTATAACCTG
    CAGAATGCCT TTAATGTGGC AGAGAAGGAG CTGGGTCTTA CCAAGCTGCT GGATCCAGAA
    GATGTGAATG TAGATCAGCC TGATGAGAAG TCCATCATCA CATATGTGGC AACATACTAC
    CACTACTTCA GCAAGATGAA GGCACTCGCC GTTGAAGGAA AGAGGATTGG CAAGGTGCTG
    GATTATGCTA TCGAGGCTGA TCAACTGATA GAGAAATATG AGACTCTGGC CTCAGAGCTG
    CTGCAGTGGA TTGAACAAAC CATCGTCACC CTAAATGATC GCCAGCTTGC TAACTCACTC
    AGTGCAGTTC AGAACCAGCT TCAAGCCTTC AACACCTACC GCACTGTGGA GAAACCACCC
    AAGTTCACTG AGAAGGGCAA TTTGGAGGTT TTGCTCTTCA CCATTCAAAG TAAAATGAGA
    GCAAACAATC AGAAGGTATA CATGCCAAGA GAGGGAAAAC TTATCTCTGA CATCAACAAG
    GCGTGGGAGA GGCTGGAGAA AGCAGAGCAT GAGAGAGAGT TGGCACTGAG AAACGAACTG
    ATTCGTCAAG AGAAGCTGGA GATGCTGGCT GCACGTTTTG ATCGCAAAGC AGCAATGAGA
    GAAACCTGGC TCAGTGAAAA CCAGCGGCTG GTGTCACAGG ACAATTTTGG TGTTGAACTG
    GGTGCTGTGG AGGCTGCCAC TCGCAAACAC GAGGCCATCG AGACGGACAT CCAGGCATAT
    GGTGAGCGTG TAGCCGCCGT AGAAGCTGTC GCTCGAGAAC TGGAAGCAGA GGGATATCAT
    GAAGTGCGAC GTGTGCTGGC GCGGAGGGAT AATGTGCTCA GGCTGTGGGA GTATCTGAAG
    GAACTGCTTG CAGCACGACG AGAGAGGCTG ATGGCACACC GTGACCTGCA GCGCCTCCTA
    CAGGAATTGA GCTACATCAT GGAATGGATG GAAGACATGA AGAGTCGTTT GCAATCTCAG
    GACAGTGGAA AACACCTTCA TGATGTGGAA GACTTGCTCC AGAAGCACAC ACTGGTGGAA
    GCTGATATAT CTGCACAGGC TGAGAGAGTC AAAGCTGTTC AGGCCACTGC AAAGAGATTT
    GCTTCAAATG AACAGAGTTA CAAGCCATGT GACCCAGCAC TGGTTGAAGA GAAAGTGGAT
    CTTCTGGGTC GAGCGTATGG AGAACTGGGC CAGCTGGCTG CGGATCGACG AGTTCGTCTA
    GATGACTCCC GAAGACTCTG GCAGTTCCTG TGGGAGCTGG GAGAAGAGGC CGCCTGGATC
    CGAGAGCAGG AGCAGATCAT CTCTGGAGGA GACTACGGTA AAGATCTGAG CTCCGCTCTT
    CACCTCCTGT CTAAACATGA GGCTTTTCGG GATGAGATGG CAGCTCGGTA TGGCCCTCTG
    GGGAACAGCA TTGCTGGTGG AGAAGCCCTG GTGAAGGAAG GCCACGTTGG AGCACCTGAG
    GTGACTGAGA GGATTAAAGA TGTGAGAGCA CAGTGGTCAC ACCTAGAGGA GGCCTCACAG
    TTGCGGGAGC AGAGCCTGAA TGAGTCTGTT GCTTTCCATC AATTCCAGAC TGACGCCAAT
    GACATGGAGG CTTGGATACT GGAAACATTA AGACAGGTCT CCAGTCAAGA AGTGGGCCAT
    GATGAGTTTT CCACACAGAC TCTGGCCAGA AAGCAGAGAG AGGTGGAAGA GGAGATTCAA
    AGTCACCGAT CACTCATAGA TTCATTACAT GAACAGGGCT CATTACTGCC TGAGGCATAT
    GCACATTCCC CACAGGTGGA GGGTCGTCTC CCAGCAATAG AGCAGCGATA TGAGGAGTTA
    GAGGGGTTGT CGTCGTCATG GCGACAGGCC TTGGATGGAG CGCTTGCGTT ATATCGCATG
    TTTAGTGAAG CTAGTGCCTG CCAACTGTGG GTTGGAGAAA AAGAGCAGTG GCTACACAAC
    ATGGAGATTC CCACCAAACT GGAGGATCTG GAGGTGATCC AGCAGAGGTT TGATACTCTG
    GAGCCCGAGA TGAACACATT GGGTGGACGC ATTTCTGATG TCAATCAAGT TGCCCAACAG
    CTGCTTGGGT CAGACAACCG CAACAAAGAC CAGATTGACC AGACACAAAA CCAACTCAAT
    AACAGGTGGT CTGATTTCCA GAGTCTGGCC AACCAGCGTA AACAAGCTCT AGAATCGGCT
    CTAAATATTC AGAACTATCA TCTGGAATGT AATGAGATCA AGAGCTGGAT GAAGGAGAAG
    ACCAAGGTCA TCGAGTCCAC ACAGAGTCTT GGAAATGACC TGGCTGGAGT TATGGCCCTA
    CAGCGCAAGC TCACTGGAAT GGAGAGAGAC CTGGAAGCCA TACAGGGTAA GTTGGATGAC
    CTGCGTTCGG AAGCCAAAAA GTTAGTATCT GAGCATCCTG AGCAGGAGGA AGAGATCAAG
    GGTCAGCTGG CTGAGATCCA GGAGGTGTGG GAGGAACTTC GTGCCACCAT GAAACGGCGT
    GAGGAGTCTT TGGGTGAAGC TTCCAAGCTT CAGGGCTTTC TTCGAGATCT TGATGACTTC
    CAGGCCTGGC TGTCTCGTAC ACAGACCACC GTGGCGTCCG AGGACACACC GACCTCTCTG
    GCGGAAGCAG AGCGTCTGCT GGCTCAACAT GAGGCCATAA AGAATGAAGT GGATAATTAT
    AAAGAAGATT ATGAGAAGAT GAGAGCTACT GGAGCTGAGG TAACTCAGGG TCAGACAGAC
    GCCCAGCACA TGTTCTTAGC CCAGAGGCTG CAAGCGCTGG ACACCGGGTG GCATGAGCTG
    AGGAGAATGT GGGAGAGCCG CCACTGTGTC CTCGCTCAGG CCTTTGATTT CCAAACCCTG
    CTGCGAGATG CAAAACAGGC AGAGGGCTTC CTTAATAGCC AGGAGTATGT TCTGTCACAT
    ACAGAGATGC CATCCAGCCT GCAAGGGGCT GAGGAGGCCA TCAAGAAACA TGAGGACTTC
    CTCACTACAA TGGAGGCCAG TGAAGAAAAG ATCAACGGTG TGGTAGAGTC TGGACGGAGA
    CTTGTATCTG ATGGCAACAC TTATGCGGAC AAGATCCAGG AGAAGACTGA CTCTATTCAG
    GAAAGGCACC AAAAGAATAA ACAAGCTGCC AATGAGCTGC TGGCTAAACT GAAGGATAAC
    AGAGAGCTGC AGCATTTCCT GCAGGATGGA CAAGAGCTTA CTTTGTGGAT AAATGAGAAG
    ATGCTGACTG CTCAGGATAT GTCCTATGAT GAGGCCAGAA ACCTCCACAG CAAGTGGCAG
    AAGCACCAGG CATTCATGGC TGAGCTGGCC TCCAATAAAG ACTGGCTGGA CAAGATCGAC
    AAGGAGGGTC AGGTGTTAGT AAAGGAGAAG CCCGAGTTGG AGCAAACTGT ATCAGAGACT
    CTGAGCGGTC TGCAGAAGCA GTGGGTAGAG CTTGAGAACA CCACCCAAGC CAAAGCTCAG
    TGTCTGTTTG ACGCTAACCG AGCGGAGCTG TTCACACAGA GCTGCTCAGC GCTGGACTCC
    TGGCTCCAGA ACATCTCCTC TCAACTCCAG AGCGACGACT TTGGAAAAGA TCTCACCAGC
    GTCAACATCC TGCTTAAAAA ACACCAGATG CTGGAGCATC AGATGGATGT GCGCGAGAAA
    GAAGTCCAGT CTCTGCAGTC ACAAGCTCTG GCCTTGGCCC AAGAAGATTC TGGGATAGTG
    GAAGTGGATG GGGAGCAGAG AAGAGTGACA GACAGATTCT CTCAGCTGCA GGACCCTCTG
    AAAGAGCGGA GGCAGCATCT TCTGGCTTCA AAAGAGGCTC ATCAGTTCAA CAGAGATCTT
    GAGGATGAGA TTTTATGGGT GAAGGAGAGG ATGCCCCTTG CCACGTCTAC AGACCATGGC
    AAAGATCTGC CCAGTGTTCA GCTGCTCATG AAAAAGAATC AGACTCTGCA GAAGGAGATT
    CAAGGTCACC AGCCCCGTAT CGATGACATC CAGGCTCACG GTACGAATAT GTCCCCTGGC
    AAAGAGTCTG AGATGGACAG GGAGAGGAGA GCTGCTCTTG ACGGCCGTCT GGCGGAGCTG
    AGAGAATTGT GGGCCCTTTT GATTTCTGAG ACTGAGCAGA GGAACGTGAG GCTAGCTGAG
    GCTAACAGAG CGCAGCAGTT TTACACGGAT GCCGCAGAGG CGGAGGCTTG GATGGGAGAA
    CAAGAGCTTC ATATGATGTC AGAGGAGAAA GCGAAGGATG AACAGAGTGC TCTAGTGATG
    GTGAAGAAAC ACCAGATTCT GGAGCAGGCT CTTGAGGACT ACGCTCAGAC CATCCACCAG
    CTGGCCAACA GCAGCAGACT CATGGTCAAC AGCGAACACC CGGAGAGTGA AAGGATCACT
    CTGAGGCAGG CCCAGGTGGA TAAACTGTAC GCAGGGTTGA AGGATCTAGC CGAGGAGAGG
    AGGGGTAAAC TGCAGGAGAG ACTGAGATTG ACCCAGCTGA AGAGAGAGGT GGATGATTTA
    GAGCAGTGGA TCGCCGAAAG AGAGGTCGTC GCTGGATCTC ATGAACTTGG ACAAGACTAC
    GAACATGTCA CGATGTTGCG TGACAAGTTC CGGGAGTTTG CGCGGGACAC CAGCACAATT
    GGCCAGGAGC GTGTAGATGC AGTAAATGCT CAGGCAGATG AACTTATCGA ATCCGGTCAT
    CCTGAAAACG CCAGTGTGGC TGAGTGGAAG GACGGACTCA ATGAGGCCTG GGCTGACCTG
    CTGGAACTCA TCGACACACG CACACAAATG CTGGCCGCCT CGTATGAGCT GCATCGCTTC
    CATCAAGACG CTCGGGAAGC TCTGGGGCTC ATACGAGAGA AGAAAGAGAC GCTAGCGGGG
    GCTGAACTCG GACGGGACTT GAATACTGTC CAGCACTTTC TCAGACAGCA TACGGCCTAT
    GAGCATGATG TGCAGGCACT CAGTGGGCAG GTCACACAGG TGCAAGATGA TGCTGCACGT
    CTGCAGAAAG CATATGCTGG AGAGAAAGCG GATGATATCC ATCGACATGA GCGTGCAGTC
    ACAGAGGCCT GGGAGGGACT TCAGTCTGCA ACTCAAACCA GGCGGCTGCT TCTATTGGAC
    ACAGTGGAGA AGTTCAGATT CCTAAACATG GTTCGAGACC TCATGCTGTG GATGGAGGGA
    ATCAACTTAC AGATCCAGTC ACACGACAGT CCGAGGGACG TCTCCTCAGC TGGTCTGGTC
    ATCGCTAACC ATCAGGACAT CAAGTCAGAG ATAGAGACTA GAGCAGACAG CTTTACTGCT
    TGCAGTGAAA TGGGACGCAC ACTCATCAAC AACAATCACT ATGCTTCAGA TGAGATTCAA
    GAAAAATTGG ACCAGCTTCA GGCCAAACGC ACTGAAATTA ACCAGAAGTG GCAAGAAAAA
    ATGGACCACT TACAGATTGT TCTGGAGGTT TTGCAGTTTG GCAGAGATGC ATCTATGGCC
    GAGTCGTGGC TGGCTGGGCA GGAGCCGCTG GTCAGGGCAG CAGAACTGGG CTCTAACGTA
    GATGAAGTTG AGAGTCTCAT CAAACGCCAC GAAGCCTTTG AAAAACTGGC AGCAGGCTGG
    GAAGATCGAT TCTCGCAGCT AGAGAAGCTC ACCACGCTGG AGGAGCAGGA AATTCAAAGA
    CGAAGGGAAG AGGAAGAGAG GGCCCGACGG CCACCCACAC CTCCCCCTGT AGAGGAAGTA
    GTACAGTCTG AGATTAATGA TTCTGCCGCA AGGACGAGTC TAGACCAGAC TACATTGAAC
    CAGTCTGTAT CTGTCAATGG TGTCTACAGT GACCAGGACA CGTCACAGGG ATCAGAGTCT
    GAATCTGTTA ACGGTCCAGG CCGTGACAGT GGTCTTGACT CAGCATCACG GCAAGACCCT
    TCAGCAACAC TGCCAGGGCG AGGAGGAGCA GAGGGGACCA CAGAAGCTAT GGAGGGCATC
    CTATGCAGGA AACAGGAAAT GGAATCGCAC AACAAAAAAT CAGCTAACAG ATCGTGGCAG
    AATGTATATT GCGTTTTACG TAAAGGCAGT CTGGGCTTCT ATAAGGACAA CAAGAGTGCC
    TCAAATGGAA TCCCCTACCA TGGTGAAGTT CCCATCAGCC TCGGGGAGGC TGTCTGTGAG
    GTTGCCCATG ACTATAAGAA GAGGAAACAC GTATTTAAAC TTAGGCTCGG GGATGGAAAA
    GAGTACTTGT TCCAAGCGAA GGATGAGGCT GAGATGAGTT CATGGATTCG AGCCATCCAG
    TCCTCCATGT CGTCCAGCGA GCGTTCGCCA GGTGCCTCCC GAGGTCTGAG TCGAGCAATG
    ACCATGCCGC CCATGTCTCC CAGCTCAGGC GAGGCCGGAG GAGTAACCAT GCGCAACAAA
    GACGGCAAAG AGAGAGACCG CGAGAAGCGC TTCAGTTTCT TCGGCAAGAA GAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.