rsph9 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rsph9
Entrez Gene ID	558005
Full Name	radial spoke head 9 homolog
Synonyms	si:ch211-117l16.2,zgc:123211
General protein information	Preferred Names radial spoke head 9 homolog Names radial spoke head protein 9 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030113.1	NP_001025284.1	radial spoke head protein 9 homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	RSPH9	NP_689945.2
Drosophila melanogaster (fruit fly)	CG31803	NP_724049.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011032	XP_307731.2
Gallus gallus (chicken)	RSPH9	NP_001264533.1
Xenopus tropicalis (tropical clawed frog)	rsph9	NP_001005021.1
Mus musculus (house mouse)	Rsph9	NP_083614.1
Bos taurus (cattle)	RSPH9	NP_001039732.1
Rattus norvegicus (Norway rat)	Rsph9	NP_001101675.1
Danio rerio (zebrafish)	rsph9	NP_001025284.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel roles for the radial spoke head protein 9 in neural and neurosensory cilia.
Sedykh I, TeSlaa JJ, Tatarsky RL, Keller AN, Toops KA, Lakkaraju A, Nyholm MK, Wolman MA, Grinblat Y
Scientific reports634437(2016 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Choksi SP, Babu D, Lau D, Yu X, Roy S
Development (Cambridge, England)141(17)3410-9(2014 Sep)

miR-34b regulates multiciliogenesis during organ formation in zebrafish.
Wang L, Fu C, Fan H, Du T, Dong M, Chen Y, Jin Y, Zhou Y, Deng M, Gu A, Jing Q, Liu T, Zhou Y
Development (Cambridge, England)140(13)2755-64(2013 Jul)

Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities.
Castleman VH, Romio L, Chodhari R, Hirst RA, de Castro SC, Parker KA, Ybot-Gonzalez P, Emes RD, Wilson SW, Wallis C, Johnson CA, Herrera RJ, Rutman A, Dixon M, Shoemark A, Bush A, Hogg C, Gardiner RM, Reish O, Greene ND, O'Callaghan C, Purton S, Chung EM, Mitchison HM
American journal of human genetics84(2)197-209(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rsph9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rsph9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00037	NM_001030113.1 Latest version!	Danio rerio radial spoke head component 9 (rsph9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rsph9 ( NM_001030113.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025284.1 Danio rerio radial spoke head component 9 (rsph9), mRNA.

Price & Availability↑

CloneID	ODa00037
Clone ID Related Accession (Same CDS sequence)	NM_001030113.1
Accession Version	NM_001030113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Danio rerio(zebrafish)
Product	radial spoke head protein 9 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649467.5. On Aug 6, 2005 this sequence version replaced XM_681169.1. ##Evidence-Data-START## Transcript exon combination :: BC109416.1, EH505942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCTG ATTCTCTGCA TTACTCCCTG GATTTAGCTG CTGCTAATGG TTTGACTCTG 
AGCAGTGAAC AGAGAGCCGC TCTTCAATCC TCTCTGCTCA TAGTCAAGAG AAACTACAAA 
TTCAGCCGGG TTTTGTTTTG GGGAAAGATT TTGGGAATAA AAAGTGACTA TTTTATTGCA 
CAAGGAGTTG AAGATGATGA ACTGAAGAAC AGGAAGTCTT TGTATAGTTT AAACTGCGTA 
GATTGGCACC TGCTCCCCCC AGCCACCGAG TCCATGATTG CAGATGTGGC ATTAGCAGCA 
ACGGGACGAT TCACAGGCGA CCCGTCCCAT GAGTACGAGC ACACAGAGAT CCGCACAGAG 
GGCGAGGGGG ACGAGGCTAC ACATGAAGAG GTCACGGTGA AAGTGATTGA AGCAAGTAGA 
CTGGCTGCAA TAGTGTCAAA CATCGATAAA GACGTGTCTG TAGTCCCACG TGGTGCATTC 
ATCAAGAGTC CCAATGGCAA AGTGCAGACA AACCGAAGCT TTGGAGGCCT TCATCCCACT 
GAGGCTGCAA AACTGCGCAA CTACCTGCAC TTCCGTGAGC CGGTTAACCT GAGGAACAAA 
TCCATCTTGG AAATGTCTGA ACTTAATCCT GCTCTTGACT TCCTAGATCC TTTGAGCGAA 
GACATTCTCA AAGGGTCATG GAGCCTGCAG CTGGATCGGG GCGGCACTGT GTGTGTATTA 
CGAAGTTTAC TATGGCTGGG TTTCACCTTC TTCCATGTTC CCCAGACTCC TCAACATGGG 
TACATATACA TGGGAGATGG CCTCATGAAC CTTGACCTGC CCTTCATGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025284.1
CDS	84..917
Translation	MDSDSLHYSLDLAAANGLTLSSEQRAALQSSLLIVKRNYKFSRVLFWGKILGIKSDYFIAQGVEDDELKNRKSLYSLNCVDWHLLPPATESMIADVALAATGRFTGDPSHEYEHTEIRTEGEGDEATHEEVTVKVIEASRLAAIVSNIDKDVSVVPRGAFIKSPNGKVQTNRSFGGLHPTEAAKLRNYLHFREPVNLRNKSILEMSELNPALDFLDPLSEDILKGSWSLQLDRGGTVCVLRSLLWLGFTFFHVPQTPQHGYIYMGDGLMNLDLPFML

Target ORF information:

RefSeq Version	NM_001030113.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio radial spoke head component 9 (rsph9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030113.1

ORF Insert Sequence:

ATGGACTCTG ATTCTCTGCA TTACTCCCTG GATTTAGCTG CTGCTAATGG TTTGACTCTG 
AGCAGTGAAC AGAGAGCCGC TCTTCAATCC TCTCTGCTCA TAGTCAAGAG AAACTACAAA 
TTCAGCCGGG TTTTGTTTTG GGGAAAGATT TTGGGAATAA AAAGTGACTA TTTTATTGCA 
CAAGGAGTTG AAGATGATGA ACTGAAGAAC AGGAAGTCTT TGTATAGTTT AAACTGCGTA 
GATTGGCACC TGCTCCCCCC AGCCACCGAG TCCATGATTG CAGATGTGGC ATTAGCAGCA 
ACGGGACGAT TCACAGGCGA CCCGTCCCAT GAGTACGAGC ACACAGAGAT CCGCACAGAG 
GGCGAGGGGG ACGAGGCTAC ACATGAAGAG GTCACGGTGA AAGTGATTGA AGCAAGTAGA 
CTGGCTGCAA TAGTGTCAAA CATCGATAAA GACGTGTCTG TAGTCCCACG TGGTGCATTC 
ATCAAGAGTC CCAATGGCAA AGTGCAGACA AACCGAAGCT TTGGAGGCCT TCATCCCACT 
GAGGCTGCAA AACTGCGCAA CTACCTGCAC TTCCGTGAGC CGGTTAACCT GAGGAACAAA 
TCCATCTTGG AAATGTCTGA ACTTAATCCT GCTCTTGACT TCCTAGATCC TTTGAGCGAA 
GACATTCTCA AAGGGTCATG GAGCCTGCAG CTGGATCGGG GCGGCACTGT GTGTGTATTA 
CGAAGTTTAC TATGGCTGGG TTTCACCTTC TTCCATGTTC CCCAGACTCC TCAACATGGG 
TACATATACA TGGGAGATGG CCTCATGAAC CTTGACCTGC CCTTCATGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel roles for the radial spoke head protein 9 in neural and neurosensory cilia.
Scientific reports634437(2016 Sep)
Sedykh I,TeSlaa JJ,Tatarsky RL,Keller AN,Toops KA,Lakkaraju A,Nyholm MK,Wolman MA,Grinblat Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Development (Cambridge, England)141(17)3410-9(2014 Sep)
Choksi SP,Babu D,Lau D,Yu X,Roy S

miR-34b regulates multiciliogenesis during organ formation in zebrafish.
Development (Cambridge, England)140(13)2755-64(2013 Jul)
Wang L,Fu C,Fan H,Du T,Dong M,Chen Y,Jin Y,Zhou Y,Deng M,Gu A,Jing Q,Liu T,Zhou Y

Mutations in radial spoke head protein genes RSPH9 and RSPH4A cause primary ciliary dyskinesia with central-microtubular-pair abnormalities.
American journal of human genetics84(2)197-209(2009 Feb)
Castleman VH,Romio L,Chodhari R,Hirst RA,de Castro SC,Parker KA,Ybot-Gonzalez P,Emes RD,Wilson SW,Wallis C,Johnson CA,Herrera RJ,Rutman A,Dixon M,Shoemark A,Bush A,Hogg C,Gardiner RM,Reish O,Greene ND,O'Callaghan C,Purton S,Chung EM,Mitchison HM

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rsph9 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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