adcy2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	adcy2b
Entrez Gene ID	557902
Full Name	adenylate cyclase 2b (brain)
Synonyms	si:dkey-266i6.1
General protein information	Preferred Names adenylate cyclase 2b (brain) Names adenylate cyclase type 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170038.3	XP_005170095.1	adenylate cyclase type 2 isoform X1
NM_001099987.1	NP_001093457.1	adenylate cyclase type 2
XM_005170039.3	XP_005170096.1	adenylate cyclase type 2 isoform X2
XM_021468083.1	XP_021323758.1	adenylate cyclase type 2 isoform X2
XM_021468084.1	XP_021323759.1	adenylate cyclase type 2 isoform X3
XM_005170038.4	XP_005170095.1	adenylate cyclase type 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADCY2	NP_065433.2
Canis lupus familiaris (dog)	ADCY2	XP_535798.3
Drosophila melanogaster (fruit fly)	Ac76E	NP_524173.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010436	XP_311511.4
Gallus gallus (chicken)	ADCY2	XP_419020.3
Danio rerio (zebrafish)	adcy2a	XP_692173.5
	ADCY2	XP_001083524.1
Bos taurus (cattle)	ADCY2	XP_002696478.1
Rattus norvegicus (Norway rat)	Adcy2	NP_112269.1
Danio rerio (zebrafish)	adcy2b	NP_001093457.1
Mus musculus (house mouse)	Adcy2	NP_705762.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
Wu CC, Tsai TH, Chang C, Lee TT, Lin C, Cheng IH, Sun MC, Chuang YJ, Chen BS
PloS one9(6)e97902(2014)

A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach.
Alves-Costa FA, Figueiredo MA, Lanes CF, Almeida DV, Marins LF, Wasko AP
Anais da Academia Brasileira de Ciencias84(2)487-94(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following adcy2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adcy2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24140	XM_005170039.3 Latest version!	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa150094	XM_021468083.1 Latest version!	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa150095	XM_021468084.1 Latest version!	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa31552	NM_001099987.1 Latest version!	Danio rerio adenylate cyclase 2b (brain) (adcy2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24139	XM_005170038.4 Latest version!	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa24139	XM_005170038.3	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adcy2b ( XM_005170039.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170096.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa24140
Clone ID Related Accession (Same CDS sequence)	XM_005170039.3
Accession Version	XM_005170039.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	adenylate cyclase type 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170039.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGGTTAGACT TCCTCTGGAG 
GAACAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170096.1
CDS	267..2918
Translation	MLRAAALSLLPWRGTHLSHPPQLSTLTEEDEPERHKQQDEDDEEVSGVQQDPMFQQTAYLLEGPEDMGVSSCGLKSPDWVYESYYRMSQQHPLIVFLLLIVMGACLALLAVFFASGLDMEEHMAFIMTVPTTLAIFLAIFILVCIESVFKKLLRLFSLLIWGCLVAMGYLFMFFGGIICPWDQVSFFLFIVFVVYTMLPFSMRDAVIASVLTSSSHTLVLSICLSNTSDHMEALVWQILANIIIFICGNVAGAYHKHLMDLALKQTYHDTCNCIKSPIKLEFEKHQQERLLLSLLPAHIARVMKAEIIQRLKGPNFSQAENTNNFHNLYVQRHTNVSILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRSERTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPKTTVLEVSFGMAFLLLALILCICFAGHILQWGKAASCTMPRLPSSSKVITNKPWIRLALTMATTALILVMAVFNMFFLGEDRMVASTSLNISNSSMDGPYGQITNSSNNKFYLPYFIYSCILGLISCSVFLRINYELKMIIMLGAVVAYNIIILQTHASILDDYSMALYKTQQPDRPGVLKDLKTMGSISLFIFFVTLLVLARQVRLPLEEQV

Target ORF information:

RefSeq Version	XM_005170039.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170039.3

ORF Insert Sequence:

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGGTTAGACT TCCTCTGGAG 
GAACAAGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy2b ( XM_021468083.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323758.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150094
Clone ID Related Accession (Same CDS sequence)	XM_021468083.1
Accession Version	XM_021468083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenylate cyclase type 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAG GCACACTAAT CATTCTGTAC GCAGACATTG TTGGATTCAC ACGTCTGGCC 
AGTGACTGTT CACCTGGAGA ACTGGTCTAC ATGCTCAATG AGCTTTTCGG CAAATTTGAC 
CAGATAGCCA AGGATAATGA CTGCATGCGG ATCAAAATTC TAGGCGACTG CTATTACTGC 
GTATCGGGGC TGCCGGACCC TCTGTTGGAC CATGCCAAGA ACTGTGTGAA GATGGGGCTG 
GACATGTGTG AGGCCATCGA GAAGGTGAGA GAAGCCACCG GTGTTGACAT TAACATGCGT 
GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT GGTGTCATCG GCCTTCAGAA ATGGCAGTAT 
GATGTTTGGT CACATGATGT CACCCTGGCC AATCACATGG AGGCGGGCGG TGTTCCTGGA 
CGGGTTCACA TCTCTTCTGT GACGCTGGAA CATTTGAATG GAGCATATAA GGTCGAGCAG 
GGCAACGGAC AAAGCAGAGA TTCATACCTG AAAGAGCACG GCATCGTCAC ATATCTAGTC 
ATCAACCCGA AGGCAGAAAA GCGCAGTCCT CTGCTTCTCT CTGCTATTCG GCCCACTATG 
TACGGAGCGA AGATGAGAGC GTCTGTCCGC ATGACGCAGT ACCTGGAGTC CTGGGGAGCT 
GCTAAACCTT TTGCTAACCT CCACCATCGA GACAGCATGA CCAATGAGAA TGGAATGATC 
AACACACGGG ATGTCCCATT AGGGCAGTAC AACTGTCAGC AAAGGTCTGA AAGAACAAAA 
TCTCAGAAGA AGAGATTTGA AGAGGAGTTA AATGAAAGAA TCATTCGCAC TATTGATGGC 
ATCAACTCAC AGAAACAGTG GCTGAAATCA GAGGATATTC AAAGGATATC TCTGTTCTTT 
CATAACAAGG CACTTGAAAA AGAGTACAGA GCCACGACTC TGCCGGCCTT CAAGTACTAC 
GTCACCTGTG CTTGTCTCAT TTTCTTCTGC ATCTTCATAG TGCAGGTTTT AGTCTTGCCA 
AAAACCACCG TTCTTGAAGT ATCGTTTGGA ATGGCCTTCC TGCTGCTCGC CTTGATCCTC 
TGCATTTGTT TTGCCGGTCA TATACTGCAA TGGGGCAAAG CTGCTTCCTG CACAATGCCC 
AGGCTCCCCA GCTCCTCTAA AGTCATCACC AACAAGCCCT GGATACGACT CGCGCTCACT 
ATGGCAACAA CTGCACTTAT CCTAGTCATG GCCGTATTCA ACATGTTTTT TTTAGGTGAA 
GACAGGATGG TTGCATCAAC TTCACTTAAT ATTTCTAACA GCAGTATGGA TGGGCCATAT 
GGTCAGATCA CAAACTCCTC AAACAACAAG TTCTACTTGC CTTATTTTAT CTACAGCTGC 
ATTTTGGGAT TGATCTCGTG CTCCGTGTTC CTGAGGATAA ACTATGAGCT GAAGATGATA 
ATTATGCTCG GCGCTGTAGT GGCTTACAAT ATCATCATTC TCCAGACTCA TGCCTCCATC 
CTGGACGACT ACAGCATGGC TCTCTACAAA ACCCAGCAAC CAGACCGGCC GGGTGTTCTG 
AAAGATCTGA AAACCATGGG CTCCATCTCA TTGTTCATTT TCTTCGTCAC TTTGCTTGTG 
CTGGCGAGGC AGAATGAATA TTACTGTAGG TTAGACTTCC TCTGGAGGAA CAAGTTTAAG 
AAAGAGTGTG AGGAGATCGA GACCATGGAG AACCTGAATC GGGTGCTTTT GGAGAACGTT 
CTACCTGCCC ATGTGGCCGA ACATTTCTTG GGCCGGAACT GGAAGAATGA GGACCTCTAC 
CATCAGTCAT ACGATACAGT GTGTGTGATG TTTGCGTCTA TCCCAGACTT CAAGGAGTTC 
TACACAGAAT CTGATGTTAA TAAAGAGGGG TTGGAGTGTC TCAGGCTCCT CAATGAGATT 
ATAGCAGATT TTGATGAGCT GCTGTCAAAG CCTAAATTCA GTGGTGTGGA GAAGATCAAA 
ACTATTGGCA GCACATACAT GGCTGCAACA GGGCTGAACG TCACCCCTGG ACTAGAGTAC 
GCGCAGTATC ACGACCGGCA GTACATGCAC ATCGGGACCA TGGTGGAGTT TGCATTCGCT 
CTGGTGGGGA AGCTTGACGT CATCAACAAA CATTCCTTTA ATGACTTCAG GATGCGAGTG 
GGAATAAACC ACGGTCCAGT AATTGCAGGG GTAATCGGAG CACAGAAACC ACAATACGAT 
ATCTGGGGGA ACACTGTAAA CGTAGCCAGC AGGATGGAGA GCACAGGGGT GCTTGGGAAA 
ATCCAGGTCA CGGAGGAGAC AAATTGGATC TTACAGACAT TGGGCTACAT GTGTTCCTGT 
CGAGGGATCA TCAACGTGAA GGGAAAGGGG GATCTGCGAA CCTTCTTCGT CCACACCGAG 
ATGACGCAGT CTTTATCACA AGGGAATGTA ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323758.1
CDS	121..2559
Translation	MYRGTLIILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRSERTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPKTTVLEVSFGMAFLLLALILCICFAGHILQWGKAASCTMPRLPSSSKVITNKPWIRLALTMATTALILVMAVFNMFFLGEDRMVASTSLNISNSSMDGPYGQITNSSNNKFYLPYFIYSCILGLISCSVFLRINYELKMIIMLGAVVAYNIIILQTHASILDDYSMALYKTQQPDRPGVLKDLKTMGSISLFIFFVTLLVLARQNEYYCRLDFLWRNKFKKECEEIETMENLNRVLLENVLPAHVAEHFLGRNWKNEDLYHQSYDTVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNVTPGLEYAQYHDRQYMHIGTMVEFAFALVGKLDVINKHSFNDFRMRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETNWILQTLGYMCSCRGIINVKGKGDLRTFFVHTEMTQSLSQGNVMP

Target ORF information:

RefSeq Version	XM_021468083.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468083.1

ORF Insert Sequence:

ATGTACAGAG GCACACTAAT CATTCTGTAC GCAGACATTG TTGGATTCAC ACGTCTGGCC 
AGTGACTGTT CACCTGGAGA ACTGGTCTAC ATGCTCAATG AGCTTTTCGG CAAATTTGAC 
CAGATAGCCA AGGATAATGA CTGCATGCGG ATCAAAATTC TAGGCGACTG CTATTACTGC 
GTATCGGGGC TGCCGGACCC TCTGTTGGAC CATGCCAAGA ACTGTGTGAA GATGGGGCTG 
GACATGTGTG AGGCCATCGA GAAGGTGAGA GAAGCCACCG GTGTTGACAT TAACATGCGT 
GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT GGTGTCATCG GCCTTCAGAA ATGGCAGTAT 
GATGTTTGGT CACATGATGT CACCCTGGCC AATCACATGG AGGCGGGCGG TGTTCCTGGA 
CGGGTTCACA TCTCTTCTGT GACGCTGGAA CATTTGAATG GAGCATATAA GGTCGAGCAG 
GGCAACGGAC AAAGCAGAGA TTCATACCTG AAAGAGCACG GCATCGTCAC ATATCTAGTC 
ATCAACCCGA AGGCAGAAAA GCGCAGTCCT CTGCTTCTCT CTGCTATTCG GCCCACTATG 
TACGGAGCGA AGATGAGAGC GTCTGTCCGC ATGACGCAGT ACCTGGAGTC CTGGGGAGCT 
GCTAAACCTT TTGCTAACCT CCACCATCGA GACAGCATGA CCAATGAGAA TGGAATGATC 
AACACACGGG ATGTCCCATT AGGGCAGTAC AACTGTCAGC AAAGGTCTGA AAGAACAAAA 
TCTCAGAAGA AGAGATTTGA AGAGGAGTTA AATGAAAGAA TCATTCGCAC TATTGATGGC 
ATCAACTCAC AGAAACAGTG GCTGAAATCA GAGGATATTC AAAGGATATC TCTGTTCTTT 
CATAACAAGG CACTTGAAAA AGAGTACAGA GCCACGACTC TGCCGGCCTT CAAGTACTAC 
GTCACCTGTG CTTGTCTCAT TTTCTTCTGC ATCTTCATAG TGCAGGTTTT AGTCTTGCCA 
AAAACCACCG TTCTTGAAGT ATCGTTTGGA ATGGCCTTCC TGCTGCTCGC CTTGATCCTC 
TGCATTTGTT TTGCCGGTCA TATACTGCAA TGGGGCAAAG CTGCTTCCTG CACAATGCCC 
AGGCTCCCCA GCTCCTCTAA AGTCATCACC AACAAGCCCT GGATACGACT CGCGCTCACT 
ATGGCAACAA CTGCACTTAT CCTAGTCATG GCCGTATTCA ACATGTTTTT TTTAGGTGAA 
GACAGGATGG TTGCATCAAC TTCACTTAAT ATTTCTAACA GCAGTATGGA TGGGCCATAT 
GGTCAGATCA CAAACTCCTC AAACAACAAG TTCTACTTGC CTTATTTTAT CTACAGCTGC 
ATTTTGGGAT TGATCTCGTG CTCCGTGTTC CTGAGGATAA ACTATGAGCT GAAGATGATA 
ATTATGCTCG GCGCTGTAGT GGCTTACAAT ATCATCATTC TCCAGACTCA TGCCTCCATC 
CTGGACGACT ACAGCATGGC TCTCTACAAA ACCCAGCAAC CAGACCGGCC GGGTGTTCTG 
AAAGATCTGA AAACCATGGG CTCCATCTCA TTGTTCATTT TCTTCGTCAC TTTGCTTGTG 
CTGGCGAGGC AGAATGAATA TTACTGTAGG TTAGACTTCC TCTGGAGGAA CAAGTTTAAG 
AAAGAGTGTG AGGAGATCGA GACCATGGAG AACCTGAATC GGGTGCTTTT GGAGAACGTT 
CTACCTGCCC ATGTGGCCGA ACATTTCTTG GGCCGGAACT GGAAGAATGA GGACCTCTAC 
CATCAGTCAT ACGATACAGT GTGTGTGATG TTTGCGTCTA TCCCAGACTT CAAGGAGTTC 
TACACAGAAT CTGATGTTAA TAAAGAGGGG TTGGAGTGTC TCAGGCTCCT CAATGAGATT 
ATAGCAGATT TTGATGAGCT GCTGTCAAAG CCTAAATTCA GTGGTGTGGA GAAGATCAAA 
ACTATTGGCA GCACATACAT GGCTGCAACA GGGCTGAACG TCACCCCTGG ACTAGAGTAC 
GCGCAGTATC ACGACCGGCA GTACATGCAC ATCGGGACCA TGGTGGAGTT TGCATTCGCT 
CTGGTGGGGA AGCTTGACGT CATCAACAAA CATTCCTTTA ATGACTTCAG GATGCGAGTG 
GGAATAAACC ACGGTCCAGT AATTGCAGGG GTAATCGGAG CACAGAAACC ACAATACGAT 
ATCTGGGGGA ACACTGTAAA CGTAGCCAGC AGGATGGAGA GCACAGGGGT GCTTGGGAAA 
ATCCAGGTCA CGGAGGAGAC AAATTGGATC TTACAGACAT TGGGCTACAT GTGTTCCTGT 
CGAGGGATCA TCAACGTGAA GGGAAAGGGG GATCTGCGAA CCTTCTTCGT CCACACCGAG 
ATGACGCAGT CTTTATCACA AGGGAATGTA ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy2b ( XM_021468084.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021323759.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X4, mRNA.

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CloneID	ODa150095
Clone ID Related Accession (Same CDS sequence)	XM_021468084.1
Accession Version	XM_021468084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenylate cyclase type 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AATATAGATG ACATGTCTAT GTTTTTGGAG 
TATGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323759.1
CDS	208..2256
Translation	MLRAAALSLLPWRGTHLSHPPQLSTLTEEDEPERHKQQDEDDEEVSGVQQDPMFQQTAYLLEGPEDMGVSSCGLKSPDWVYESYYRMSQQHPLIVFLLLIVMGACLALLAVFFASGLDMEEHMAFIMTVPTTLAIFLAIFILVCIESVFKKLLRLFSLLIWGCLVAMGYLFMFFGGIICPWDQVSFFLFIVFVVYTMLPFSMRDAVIASVLTSSSHTLVLSICLSNTSDHMEALVWQILANIIIFICGNVAGAYHKHLMDLALKQTYHDTCNCIKSPIKLEFEKHQQERLLLSLLPAHIARVMKAEIIQRLKGPNFSQAENTNNFHNLYVQRHTNVSILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRSERTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPNIDDMSMFLEYG

Target ORF information:

RefSeq Version	XM_021468084.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468084.1

ORF Insert Sequence:

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AATATAGATG ACATGTCTAT GTTTTTGGAG 
TATGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy2b ( NM_001099987.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093457.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), mRNA.

Price & Availability↑

CloneID	ODa31552
Clone ID Related Accession (Same CDS sequence)	NM_001099987.1
Accession Version	NM_001099987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Danio rerio(zebrafish)
Product	adenylate cyclase type 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX511155.25 and CR759914.21. On Jul 26, 2007 this sequence version replaced XM_681057.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGAACAAA ATCTCAGAAG AAGAGATTTG AAGAGGAGTT AAATGAAAGA 
ATCATTCGCA CTATTGATGG CATCAACTCA CAGAAACAGT GGCTGAAATC AGAGGATATT 
CAAAGGATAT CTCTGTTCTT TCATAACAAG GCACTTGAAA AAGAGTACAG AGCCACGACT 
CTGCCGGCCT TCAAGTACTA CGTCACCTGT GCTTGTCTCA TTTTCTTCTG CATCTTCATA 
GTGCAGGTTT TAGTCTTGCC AAAAACCACC GTTCTTGAAG TATCGTTTGG AATGGCCTTC 
CTGCTGCTCG CCTTGATCCT CTGCATTTGT TTTGCCGGTC ATATACTGCA ATGGGGCAAA 
GCTGCTTCCT GCACAATGCC CAGGCTCCCC AGCTCCTCTA AAGTCATCAC CAACAAGCCC 
TGGATACGAC TCGCGCTCAC TATGGCAACA ACTGCACTTA TCCTAGTCAT GGCCGTATTC 
AACATGTTTT TTTTAGGTGA AGACAGGATG GTTGCATCAA CTTCACTTAA TATTTCTAAC 
AGCAGTATGG ATGGGCCATA TGGTCAGATC ACAAACTCCT CAAACAACAA GTTCTACTTG 
CCTTATTTTA TCTACAGCTG CATTTTGGGA TTGATCTCGT GCTCCGTGTT CCTGAGGATA 
AACTATGAGC TGAAGATGAT AATTATGCTC GGCGCTGTAG TGGCTTACAA TATCATCATT 
CTCCAGACTC ATGCCTCCAT CCTGGACGAC TACAGCATGG CTCTCTACAA AACCCAGCAA 
CCAGACCGGC CGGGTGTTCT GAAAGATCTG AAAACCATGG GCTCCATCTC ATTGTTCATT 
TTCTTCGTCA CTTTGCTTGT GCTGGCGAGG CAGAATGAAT ATTACTGTAG GTTAGACTTC 
CTCTGGAGGA ACAAGTTTAA GAAAGAGTGT GAGGAGATCG AGACCATGGA GAACCTGAAT 
CGGGTGCTTT TGGAGAACGT TCTACCTGCC CATGTGGCCG AACATTTCTT GGGCCGGAAC 
TGGAAGAATG AGGACCTCTA CCATCAGTCA TACGATACAG TGTGTGTGAT GTTTGCGTCT 
ATCCCAGACT TCAAGGAGTT CTACACAGAA TCTGATGTTA ATAAAGAGGG GTTGGAGTGT 
CTCAGGCTCC TCAATGAGAT TATAGCAGAT TTTGATGAGC TGCTGTCAAA GCCTAAATTC 
AGTGGTGTGG AGAAGATCAA AACTATTGGC AGCACATACA TGGCTGCAAC AGGGCTGAAC 
GTCACCCCTG GACTAGAGTA CGCGCAGTAT CACGACCGGC AGTACATGCA CATCGGGACC 
ATGGTGGAGT TTGCATTCGC TCTGGTGGGG AAGCTTGACG TCATCAACAA ACATTCCTTT 
AATGACTTCA GGATGCGAGT GGGAATAAAC CACGGTCCAG TAATTGCAGG GGTAATCGGA 
GCACAGAAAC CACAATACGA TATCTGGGGG AACACTGTAA ACGTAGCCAG CAGGATGGAG 
AGCACAGGGG TGCTTGGGAA AATCCAGGTC ACGGAGGAGA CAAATTGGAT CTTACAGACA 
TTGGGCTACA TGTGTTCCTG TCGAGGGATC ATCAACGTGA AGGGAAAGGG GGATCTGCGA 
ACCTTCTTCG TCCACACCGA GATGACGCAG TCTTTATCAC AAGGGAATGT AATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093457.1
CDS	1..3420
Translation	MLRAAALSLLPWRGTHLSHPPQLSTLTEEDEPERHKQQDEDDEEVSGVQQDPMFQQTAYLLEGPEDMGVSSCGLKSPDWVYESYYRMSQQHPLIVFLLLIVMGACLALLAVFFASGLDMEEHMAFIMTVPTTLAIFLAIFILVCIESVFKKLLRLFSLLIWGCLVAMGYLFMFFGGIICPWDQVSFFLFIVFVVYTMLPFSMRDAVIASVLTSSSHTLVLSICLSNTSDHMEALVWQILANIIIFICGNVAGAYHKHLMDLALKQTYHDTCNCIKSPIKLEFEKHQQERLLLSLLPAHIARVMKAEIIQRLKGPNFSQAENTNNFHNLYVQRHTNVSILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPKTTVLEVSFGMAFLLLALILCICFAGHILQWGKAASCTMPRLPSSSKVITNKPWIRLALTMATTALILVMAVFNMFFLGEDRMVASTSLNISNSSMDGPYGQITNSSNNKFYLPYFIYSCILGLISCSVFLRINYELKMIIMLGAVVAYNIIILQTHASILDDYSMALYKTQQPDRPGVLKDLKTMGSISLFIFFVTLLVLARQNEYYCRLDFLWRNKFKKECEEIETMENLNRVLLENVLPAHVAEHFLGRNWKNEDLYHQSYDTVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNVTPGLEYAQYHDRQYMHIGTMVEFAFALVGKLDVINKHSFNDFRMRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETNWILQTLGYMCSCRGIINVKGKGDLRTFFVHTEMTQSLSQGNVMP

Target ORF information:

RefSeq Version	NM_001099987.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099987.1

ORF Insert Sequence:

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGAACAAA ATCTCAGAAG AAGAGATTTG AAGAGGAGTT AAATGAAAGA 
ATCATTCGCA CTATTGATGG CATCAACTCA CAGAAACAGT GGCTGAAATC AGAGGATATT 
CAAAGGATAT CTCTGTTCTT TCATAACAAG GCACTTGAAA AAGAGTACAG AGCCACGACT 
CTGCCGGCCT TCAAGTACTA CGTCACCTGT GCTTGTCTCA TTTTCTTCTG CATCTTCATA 
GTGCAGGTTT TAGTCTTGCC AAAAACCACC GTTCTTGAAG TATCGTTTGG AATGGCCTTC 
CTGCTGCTCG CCTTGATCCT CTGCATTTGT TTTGCCGGTC ATATACTGCA ATGGGGCAAA 
GCTGCTTCCT GCACAATGCC CAGGCTCCCC AGCTCCTCTA AAGTCATCAC CAACAAGCCC 
TGGATACGAC TCGCGCTCAC TATGGCAACA ACTGCACTTA TCCTAGTCAT GGCCGTATTC 
AACATGTTTT TTTTAGGTGA AGACAGGATG GTTGCATCAA CTTCACTTAA TATTTCTAAC 
AGCAGTATGG ATGGGCCATA TGGTCAGATC ACAAACTCCT CAAACAACAA GTTCTACTTG 
CCTTATTTTA TCTACAGCTG CATTTTGGGA TTGATCTCGT GCTCCGTGTT CCTGAGGATA 
AACTATGAGC TGAAGATGAT AATTATGCTC GGCGCTGTAG TGGCTTACAA TATCATCATT 
CTCCAGACTC ATGCCTCCAT CCTGGACGAC TACAGCATGG CTCTCTACAA AACCCAGCAA 
CCAGACCGGC CGGGTGTTCT GAAAGATCTG AAAACCATGG GCTCCATCTC ATTGTTCATT 
TTCTTCGTCA CTTTGCTTGT GCTGGCGAGG CAGAATGAAT ATTACTGTAG GTTAGACTTC 
CTCTGGAGGA ACAAGTTTAA GAAAGAGTGT GAGGAGATCG AGACCATGGA GAACCTGAAT 
CGGGTGCTTT TGGAGAACGT TCTACCTGCC CATGTGGCCG AACATTTCTT GGGCCGGAAC 
TGGAAGAATG AGGACCTCTA CCATCAGTCA TACGATACAG TGTGTGTGAT GTTTGCGTCT 
ATCCCAGACT TCAAGGAGTT CTACACAGAA TCTGATGTTA ATAAAGAGGG GTTGGAGTGT 
CTCAGGCTCC TCAATGAGAT TATAGCAGAT TTTGATGAGC TGCTGTCAAA GCCTAAATTC 
AGTGGTGTGG AGAAGATCAA AACTATTGGC AGCACATACA TGGCTGCAAC AGGGCTGAAC 
GTCACCCCTG GACTAGAGTA CGCGCAGTAT CACGACCGGC AGTACATGCA CATCGGGACC 
ATGGTGGAGT TTGCATTCGC TCTGGTGGGG AAGCTTGACG TCATCAACAA ACATTCCTTT 
AATGACTTCA GGATGCGAGT GGGAATAAAC CACGGTCCAG TAATTGCAGG GGTAATCGGA 
GCACAGAAAC CACAATACGA TATCTGGGGG AACACTGTAA ACGTAGCCAG CAGGATGGAG 
AGCACAGGGG TGCTTGGGAA AATCCAGGTC ACGGAGGAGA CAAATTGGAT CTTACAGACA 
TTGGGCTACA TGTGTTCCTG TCGAGGGATC ATCAACGTGA AGGGAAAGGG GGATCTGCGA 
ACCTTCTTCG TCCACACCGA GATGACGCAG TCTTTATCAC AAGGGAATGT AATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy2b ( XM_005170038.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170095.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24139
Clone ID Related Accession (Same CDS sequence)	XM_005170038.3 , XM_005170038.4
Accession Version	XM_005170038.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	adenylate cyclase type 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGAATGAATA TTACTGTAGG 
TTAGACTTCC TCTGGAGGAA CAAGTTTAAG AAAGAGTGTG AGGAGATCGA GACCATGGAG 
AACCTGAATC GGGTGCTTTT GGAGAACGTT CTACCTGCCC ATGTGGCCGA ACATTTCTTG 
GGCCGGAACT GGAAGAATGA GGACCTCTAC CATCAGTCAT ACGATACAGT GTGTGTGATG 
TTTGCGTCTA TCCCAGACTT CAAGGAGTTC TACACAGAAT CTGATGTTAA TAAAGAGGGG 
TTGGAGTGTC TCAGGCTCCT CAATGAGATT ATAGCAGATT TTGATGAGCT GCTGTCAAAG 
CCTAAATTCA GTGGTGTGGA GAAGATCAAA ACTATTGGCA GCACATACAT GGCTGCAACA 
GGGCTGAACG TCACCCCTGG ACTAGAGTAC GCGCAGTATC ACGACCGGCA GTACATGCAC 
ATCGGGACCA TGGTGGAGTT TGCATTCGCT CTGGTGGGGA AGCTTGACGT CATCAACAAA 
CATTCCTTTA ATGACTTCAG GATGCGAGTG GGAATAAACC ACGGTCCAGT AATTGCAGGG 
GTAATCGGAG CACAGAAACC ACAATACGAT ATCTGGGGGA ACACTGTAAA CGTAGCCAGC 
AGGATGGAGA GCACAGGGGT GCTTGGGAAA ATCCAGGTCA CGGAGGAGAC AAATTGGATC 
TTACAGACAT TGGGCTACAT GTGTTCCTGT CGAGGGATCA TCAACGTGAA GGGAAAGGGG 
GATCTGCGAA CCTTCTTCGT CCACACCGAG ATGACGCAGT CTTTATCACA AGGGAATGTA 
ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170095.1
CDS	261..3689
Translation	MLRAAALSLLPWRGTHLSHPPQLSTLTEEDEPERHKQQDEDDEEVSGVQQDPMFQQTAYLLEGPEDMGVSSCGLKSPDWVYESYYRMSQQHPLIVFLLLIVMGACLALLAVFFASGLDMEEHMAFIMTVPTTLAIFLAIFILVCIESVFKKLLRLFSLLIWGCLVAMGYLFMFFGGIICPWDQVSFFLFIVFVVYTMLPFSMRDAVIASVLTSSSHTLVLSICLSNTSDHMEALVWQILANIIIFICGNVAGAYHKHLMDLALKQTYHDTCNCIKSPIKLEFEKHQQERLLLSLLPAHIARVMKAEIIQRLKGPNFSQAENTNNFHNLYVQRHTNVSILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRSERTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPKTTVLEVSFGMAFLLLALILCICFAGHILQWGKAASCTMPRLPSSSKVITNKPWIRLALTMATTALILVMAVFNMFFLGEDRMVASTSLNISNSSMDGPYGQITNSSNNKFYLPYFIYSCILGLISCSVFLRINYELKMIIMLGAVVAYNIIILQTHASILDDYSMALYKTQQPDRPGVLKDLKTMGSISLFIFFVTLLVLARQNEYYCRLDFLWRNKFKKECEEIETMENLNRVLLENVLPAHVAEHFLGRNWKNEDLYHQSYDTVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNVTPGLEYAQYHDRQYMHIGTMVEFAFALVGKLDVINKHSFNDFRMRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETNWILQTLGYMCSCRGIINVKGKGDLRTFFVHTEMTQSLSQGNVMP

Target ORF information:

RefSeq Version	XM_005170038.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170038.3

ORF Insert Sequence:

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGAATGAATA TTACTGTAGG 
TTAGACTTCC TCTGGAGGAA CAAGTTTAAG AAAGAGTGTG AGGAGATCGA GACCATGGAG 
AACCTGAATC GGGTGCTTTT GGAGAACGTT CTACCTGCCC ATGTGGCCGA ACATTTCTTG 
GGCCGGAACT GGAAGAATGA GGACCTCTAC CATCAGTCAT ACGATACAGT GTGTGTGATG 
TTTGCGTCTA TCCCAGACTT CAAGGAGTTC TACACAGAAT CTGATGTTAA TAAAGAGGGG 
TTGGAGTGTC TCAGGCTCCT CAATGAGATT ATAGCAGATT TTGATGAGCT GCTGTCAAAG 
CCTAAATTCA GTGGTGTGGA GAAGATCAAA ACTATTGGCA GCACATACAT GGCTGCAACA 
GGGCTGAACG TCACCCCTGG ACTAGAGTAC GCGCAGTATC ACGACCGGCA GTACATGCAC 
ATCGGGACCA TGGTGGAGTT TGCATTCGCT CTGGTGGGGA AGCTTGACGT CATCAACAAA 
CATTCCTTTA ATGACTTCAG GATGCGAGTG GGAATAAACC ACGGTCCAGT AATTGCAGGG 
GTAATCGGAG CACAGAAACC ACAATACGAT ATCTGGGGGA ACACTGTAAA CGTAGCCAGC 
AGGATGGAGA GCACAGGGGT GCTTGGGAAA ATCCAGGTCA CGGAGGAGAC AAATTGGATC 
TTACAGACAT TGGGCTACAT GTGTTCCTGT CGAGGGATCA TCAACGTGAA GGGAAAGGGG 
GATCTGCGAA CCTTCTTCGT CCACACCGAG ATGACGCAGT CTTTATCACA AGGGAATGTA 
ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adcy2b ( XM_005170038.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170095.1 Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24139
Clone ID Related Accession (Same CDS sequence)	XM_005170038.3 , XM_005170038.4
Accession Version	XM_005170038.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenylate cyclase type 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170038.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGAATGAATA TTACTGTAGG 
TTAGACTTCC TCTGGAGGAA CAAGTTTAAG AAAGAGTGTG AGGAGATCGA GACCATGGAG 
AACCTGAATC GGGTGCTTTT GGAGAACGTT CTACCTGCCC ATGTGGCCGA ACATTTCTTG 
GGCCGGAACT GGAAGAATGA GGACCTCTAC CATCAGTCAT ACGATACAGT GTGTGTGATG 
TTTGCGTCTA TCCCAGACTT CAAGGAGTTC TACACAGAAT CTGATGTTAA TAAAGAGGGG 
TTGGAGTGTC TCAGGCTCCT CAATGAGATT ATAGCAGATT TTGATGAGCT GCTGTCAAAG 
CCTAAATTCA GTGGTGTGGA GAAGATCAAA ACTATTGGCA GCACATACAT GGCTGCAACA 
GGGCTGAACG TCACCCCTGG ACTAGAGTAC GCGCAGTATC ACGACCGGCA GTACATGCAC 
ATCGGGACCA TGGTGGAGTT TGCATTCGCT CTGGTGGGGA AGCTTGACGT CATCAACAAA 
CATTCCTTTA ATGACTTCAG GATGCGAGTG GGAATAAACC ACGGTCCAGT AATTGCAGGG 
GTAATCGGAG CACAGAAACC ACAATACGAT ATCTGGGGGA ACACTGTAAA CGTAGCCAGC 
AGGATGGAGA GCACAGGGGT GCTTGGGAAA ATCCAGGTCA CGGAGGAGAC AAATTGGATC 
TTACAGACAT TGGGCTACAT GTGTTCCTGT CGAGGGATCA TCAACGTGAA GGGAAAGGGG 
GATCTGCGAA CCTTCTTCGT CCACACCGAG ATGACGCAGT CTTTATCACA AGGGAATGTA 
ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170095.1
CDS	186..3614
Translation	MLRAAALSLLPWRGTHLSHPPQLSTLTEEDEPERHKQQDEDDEEVSGVQQDPMFQQTAYLLEGPEDMGVSSCGLKSPDWVYESYYRMSQQHPLIVFLLLIVMGACLALLAVFFASGLDMEEHMAFIMTVPTTLAIFLAIFILVCIESVFKKLLRLFSLLIWGCLVAMGYLFMFFGGIICPWDQVSFFLFIVFVVYTMLPFSMRDAVIASVLTSSSHTLVLSICLSNTSDHMEALVWQILANIIIFICGNVAGAYHKHLMDLALKQTYHDTCNCIKSPIKLEFEKHQQERLLLSLLPAHIARVMKAEIIQRLKGPNFSQAENTNNFHNLYVQRHTNVSILYADIVGFTRLASDCSPGELVYMLNELFGKFDQIAKDNDCMRIKILGDCYYCVSGLPDPLLDHAKNCVKMGLDMCEAIEKVREATGVDINMRVGVHSGNVLCGVIGLQKWQYDVWSHDVTLANHMEAGGVPGRVHISSVTLEHLNGAYKVEQGNGQSRDSYLKEHGIVTYLVINPKAEKRSPLLLSAIRPTMYGAKMRASVRMTQYLESWGAAKPFANLHHRDSMTNENGMINTRDVPLGQYNCQQRSERTKSQKKRFEEELNERIIRTIDGINSQKQWLKSEDIQRISLFFHNKALEKEYRATTLPAFKYYVTCACLIFFCIFIVQVLVLPKTTVLEVSFGMAFLLLALILCICFAGHILQWGKAASCTMPRLPSSSKVITNKPWIRLALTMATTALILVMAVFNMFFLGEDRMVASTSLNISNSSMDGPYGQITNSSNNKFYLPYFIYSCILGLISCSVFLRINYELKMIIMLGAVVAYNIIILQTHASILDDYSMALYKTQQPDRPGVLKDLKTMGSISLFIFFVTLLVLARQNEYYCRLDFLWRNKFKKECEEIETMENLNRVLLENVLPAHVAEHFLGRNWKNEDLYHQSYDTVCVMFASIPDFKEFYTESDVNKEGLECLRLLNEIIADFDELLSKPKFSGVEKIKTIGSTYMAATGLNVTPGLEYAQYHDRQYMHIGTMVEFAFALVGKLDVINKHSFNDFRMRVGINHGPVIAGVIGAQKPQYDIWGNTVNVASRMESTGVLGKIQVTEETNWILQTLGYMCSCRGIINVKGKGDLRTFFVHTEMTQSLSQGNVMP

Target ORF information:

RefSeq Version	XM_005170038.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate cyclase 2b (brain) (adcy2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170038.4

ORF Insert Sequence:

ATGCTGCGTG CGGCAGCGCT CTCTCTCCTG CCCTGGCGCG GGACGCATCT GTCCCACCCG 
CCGCAGCTGA GCACCCTCAC AGAAGAGGAC GAGCCGGAGC GACACAAGCA GCAGGACGAG 
GACGACGAGG AGGTCTCAGG GGTCCAGCAA GACCCTATGT TCCAACAGAC CGCTTACCTG 
CTGGAGGGTC CCGAGGACAT GGGGGTGTCG TCCTGCGGGC TGAAGTCTCC AGATTGGGTC 
TACGAGTCCT ACTACCGAAT GAGCCAGCAG CATCCACTCA TCGTGTTCCT GTTGCTCATC 
GTGATGGGAG CGTGCCTGGC ACTGCTCGCC GTGTTTTTCG CCTCTGGGCT GGACATGGAG 
GAACACATGG CCTTCATCAT GACTGTGCCT ACAACACTGG CCATTTTTTT GGCCATCTTC 
ATTCTTGTCT GCATCGAGTC TGTCTTCAAA AAGCTCCTTC GCCTCTTCTC CCTGCTCATC 
TGGGGCTGTC TGGTTGCCAT GGGTTACCTC TTCATGTTTT TCGGTGGGAT CATCTGTCCG 
TGGGACCAGG TGTCGTTTTT CCTGTTTATT GTGTTTGTGG TCTACACCAT GCTGCCGTTC 
TCCATGAGGG ATGCCGTCAT CGCTAGTGTC CTCACTTCAT CCTCCCACAC GCTAGTGCTC 
AGCATCTGCC TCTCCAACAC TAGCGACCAC ATGGAGGCCC TCGTCTGGCA GATCCTTGCC 
AACATCATCA TCTTCATCTG TGGAAATGTG GCAGGGGCCT ATCACAAACA CCTGATGGAT 
CTGGCTCTTA AACAGACGTA CCACGACACC TGCAACTGCA TCAAGTCTCC CATCAAACTG 
GAATTTGAGA AGCATCAGCA GGAGCGTCTT TTACTATCCC TGCTTCCCGC CCACATCGCA 
CGGGTCATGA AGGCGGAGAT CATCCAGAGA CTGAAGGGAC CCAACTTCAG CCAGGCGGAA 
AACACCAACA ACTTCCACAA CCTCTATGTA CAGAGGCACA CTAATGTAAG CATTCTGTAC 
GCAGACATTG TTGGATTCAC ACGTCTGGCC AGTGACTGTT CACCTGGAGA ACTGGTCTAC 
ATGCTCAATG AGCTTTTCGG CAAATTTGAC CAGATAGCCA AGGATAATGA CTGCATGCGG 
ATCAAAATTC TAGGCGACTG CTATTACTGC GTATCGGGGC TGCCGGACCC TCTGTTGGAC 
CATGCCAAGA ACTGTGTGAA GATGGGGCTG GACATGTGTG AGGCCATCGA GAAGGTGAGA 
GAAGCCACCG GTGTTGACAT TAACATGCGT GTTGGAGTGC ATTCTGGAAA TGTTCTCTGT 
GGTGTCATCG GCCTTCAGAA ATGGCAGTAT GATGTTTGGT CACATGATGT CACCCTGGCC 
AATCACATGG AGGCGGGCGG TGTTCCTGGA CGGGTTCACA TCTCTTCTGT GACGCTGGAA 
CATTTGAATG GAGCATATAA GGTCGAGCAG GGCAACGGAC AAAGCAGAGA TTCATACCTG 
AAAGAGCACG GCATCGTCAC ATATCTAGTC ATCAACCCGA AGGCAGAAAA GCGCAGTCCT 
CTGCTTCTCT CTGCTATTCG GCCCACTATG TACGGAGCGA AGATGAGAGC GTCTGTCCGC 
ATGACGCAGT ACCTGGAGTC CTGGGGAGCT GCTAAACCTT TTGCTAACCT CCACCATCGA 
GACAGCATGA CCAATGAGAA TGGAATGATC AACACACGGG ATGTCCCATT AGGGCAGTAC 
AACTGTCAGC AAAGGTCTGA AAGAACAAAA TCTCAGAAGA AGAGATTTGA AGAGGAGTTA 
AATGAAAGAA TCATTCGCAC TATTGATGGC ATCAACTCAC AGAAACAGTG GCTGAAATCA 
GAGGATATTC AAAGGATATC TCTGTTCTTT CATAACAAGG CACTTGAAAA AGAGTACAGA 
GCCACGACTC TGCCGGCCTT CAAGTACTAC GTCACCTGTG CTTGTCTCAT TTTCTTCTGC 
ATCTTCATAG TGCAGGTTTT AGTCTTGCCA AAAACCACCG TTCTTGAAGT ATCGTTTGGA 
ATGGCCTTCC TGCTGCTCGC CTTGATCCTC TGCATTTGTT TTGCCGGTCA TATACTGCAA 
TGGGGCAAAG CTGCTTCCTG CACAATGCCC AGGCTCCCCA GCTCCTCTAA AGTCATCACC 
AACAAGCCCT GGATACGACT CGCGCTCACT ATGGCAACAA CTGCACTTAT CCTAGTCATG 
GCCGTATTCA ACATGTTTTT TTTAGGTGAA GACAGGATGG TTGCATCAAC TTCACTTAAT 
ATTTCTAACA GCAGTATGGA TGGGCCATAT GGTCAGATCA CAAACTCCTC AAACAACAAG 
TTCTACTTGC CTTATTTTAT CTACAGCTGC ATTTTGGGAT TGATCTCGTG CTCCGTGTTC 
CTGAGGATAA ACTATGAGCT GAAGATGATA ATTATGCTCG GCGCTGTAGT GGCTTACAAT 
ATCATCATTC TCCAGACTCA TGCCTCCATC CTGGACGACT ACAGCATGGC TCTCTACAAA 
ACCCAGCAAC CAGACCGGCC GGGTGTTCTG AAAGATCTGA AAACCATGGG CTCCATCTCA 
TTGTTCATTT TCTTCGTCAC TTTGCTTGTG CTGGCGAGGC AGAATGAATA TTACTGTAGG 
TTAGACTTCC TCTGGAGGAA CAAGTTTAAG AAAGAGTGTG AGGAGATCGA GACCATGGAG 
AACCTGAATC GGGTGCTTTT GGAGAACGTT CTACCTGCCC ATGTGGCCGA ACATTTCTTG 
GGCCGGAACT GGAAGAATGA GGACCTCTAC CATCAGTCAT ACGATACAGT GTGTGTGATG 
TTTGCGTCTA TCCCAGACTT CAAGGAGTTC TACACAGAAT CTGATGTTAA TAAAGAGGGG 
TTGGAGTGTC TCAGGCTCCT CAATGAGATT ATAGCAGATT TTGATGAGCT GCTGTCAAAG 
CCTAAATTCA GTGGTGTGGA GAAGATCAAA ACTATTGGCA GCACATACAT GGCTGCAACA 
GGGCTGAACG TCACCCCTGG ACTAGAGTAC GCGCAGTATC ACGACCGGCA GTACATGCAC 
ATCGGGACCA TGGTGGAGTT TGCATTCGCT CTGGTGGGGA AGCTTGACGT CATCAACAAA 
CATTCCTTTA ATGACTTCAG GATGCGAGTG GGAATAAACC ACGGTCCAGT AATTGCAGGG 
GTAATCGGAG CACAGAAACC ACAATACGAT ATCTGGGGGA ACACTGTAAA CGTAGCCAGC 
AGGATGGAGA GCACAGGGGT GCTTGGGAAA ATCCAGGTCA CGGAGGAGAC AAATTGGATC 
TTACAGACAT TGGGCTACAT GTGTTCCTGT CGAGGGATCA TCAACGTGAA GGGAAAGGGG 
GATCTGCGAA CCTTCTTCGT CCACACCGAG ATGACGCAGT CTTTATCACA AGGGAATGTA 
ATGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

On the crucial cerebellar wound healing-related pathways and their cross-talks after traumatic brain injury in Danio rerio.
PloS one9(6)e97902(2014)
Wu CC,Tsai TH,Chang C,Lee TT,Lin C,Cheng IH,Sun MC,Chuang YJ,Chen BS

A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach.
Anais da Academia Brasileira de Ciencias84(2)487-94(2012 Jun)
Alves-Costa FA,Figueiredo MA,Lanes CF,Almeida DV,Marins LF,Wasko AP

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adcy2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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