ube4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube4a
Entrez Gene ID	557121
Full Name	ubiquitination factor E4A (UFD2 homolog, yeast)
Synonyms	zgc:162146
General protein information	Preferred Names ubiquitination factor E4A (UFD2 homolog, yeast) Names ubiquitin conjugation factor E4 A ubiquitination factor E 4A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291737.2	XP_009290012.1	ubiquitin conjugation factor E4 A isoform X1
XM_003200047.5	XP_003200095.2	ubiquitin conjugation factor E4 A isoform X2
XM_003200047.4	XP_003200095.2	ubiquitin conjugation factor E4 A isoform X2
XM_009291737.3	XP_009290012.1	ubiquitin conjugation factor E4 A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE4A	NP_004779.2
Bos taurus (cattle)	UBE4A	NP_001092469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001681	XP_321414.5
Pan troglodytes (chimpanzee)	UBE4A	XP_508791.2
Xenopus tropicalis (tropical clawed frog)	ube4a	NP_001163971.1
Canis lupus familiaris (dog)	UBE4A	XP_850081.1
Mus musculus (house mouse)	Ube4a	NP_663375.3
Danio rerio (zebrafish)	ube4a	XP_001922961.2
Drosophila melanogaster (fruit fly)	CG11070	NP_609060.1
	UBE4A	XP_001097911.2
Rattus norvegicus (Norway rat)	Ube4a	NP_997493.1
Gallus gallus (chicken)	UBE4A	NP_001026081.1

Gene Ontology

The following ube4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20491	XM_009291737.3 Latest version!	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa20492	XM_003200047.5 Latest version!	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa20492	XM_003200047.4	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa20491	XM_009291737.2	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube4a ( XM_009291737.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290012.1 Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20491
Clone ID Related Accession (Same CDS sequence)	XM_009291737.2 , XM_009291737.3
Accession Version	XM_009291737.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATCAG TAGATTCAGG AGAGAGCCAG TCTGAGTCAG AAAACTCTGC GTCTGAAAGC 
ATTGATGAGA ATGATGACTC AGTGGCGGAG ATCAGCCGAT CGTTCCGCTC ACGACAGGAG 
CTCTGTGAAC AGCTCAATGT CAACCACATG ATCCAGAGGA TCTTCCTCAT CACTCTGGAC 
AACAGTGATC CGAGTCTAAG AAGTGGCAAT GGCATCCCAC CACGCTGTGT TTACCTTGAG 
GAAATGGCAG CTGATCTTGA TGGTCAGGAC TGGCTTAACA TGGACACCAT AGAGCAGGCT 
TTGTTCAACA GGCTGCTTTT GCAGGAGCCA GGAAACCATC TCATTTACAT GACCTCCTGT 
AGTGTAGTGA ATCTCTCGGC AGATCGAGAC GCTGGAGAGA AACGTGCCAT ACCTTACCTT 
TACGCCTGCT ACCAAAGAGC TAAAGAGGAG ATCACTAAAG TCCCTGAGAA ACTGTTGTCA 
TTTGCTGTGC ACTGCAAAAA CCTGACCGTG TCCAATGCAC GAACTGTGCT GCTGACCCCA 
GAGATCTACA TCAGTCAGAA CGTTTACGAG CAGCTTCTGG ACCTGTTGCT GGAGGCTGTC 
AGAGGAGCCC AGTTTGAAGA GGTTGTGGAG TTTTTAGAGG AGGTCATTGC TAGCCTGTTG 
GCTGACCAAG AAGTGCGGAC CTTTGGGGAG GTAATGGTGC CAGTGTTTGA TATTTTCCAT 
GGCCGGGTTA AAGACCTAGA CCTCTGTCAG CTGCTGCTGT TTTCCTACCT GGAAATTCTC 
CTGTACTTCA GTCGACAAAA GGACATTGCT AAGGTTTTAA TGGAGCACAT TCAGCCCAAA 
GACCCTAACA ATGGGATTCA GTATCAGAAA ACACTTCTGG GAGCCATCCT TAATATTTCA 
TGTCTGCTGA AAACTCCTGG TGTGGTTGAA AACCATGGCT TTTTCCTGAA TCCATCTCGC 
TCCAGCCCAC AGGAATTGAA GGTTCAAGAG TCAAATATAC ACCAGTTTAT GGGGCAGTTT 
CATGATAAGC TCTATCAGAT CCTGAAAAAC TTGCTCCAGC AGTCCGGCGA GACTCGCCAC 
CTGCTGCTCT CGTGGCTGGG TGGCTGCCTG CAGGCCAACA TGGGTCGGGC CAAAATCTGG 
GCAAACCAGA TGCCCGAGAT ATTCTTCCAA ATGTATGCAT CGGATGCATT CTTCTTAAAT 
CTGGGAGCCG CTCTGCTCAA GCTCTGCCAG CCGTTTTCTC GACCCTACTC TCCTAAACTC 
CTGACCTTTA ACCCCACATA CTGCTTGCTT AAGGAGCTGA GCGAAGAAGA GCGGCGTAAC 
AGAAACGTGC ATGCTAGAGG TCTGGACAAG GAGACATGTC TGATACCTGT GCCCCCTCAA 
CAAACGGTTG AATTTGCCCA ATCATACAGC CTTCTGACAG AAAACCTCAT TCTTACACAA 
CTCACCCTGT ACCTTGGCTT CCATAGACTT CATGATCAGA TGGTGAAGAT GAATCAGTCT 
CTGCACCGGC TCCAGGGGAC GTGGCGGGAC ACGCAGCTCA GTGGAGGCCC AGCAGCAGCA 
GAGCTGCGGG AACAGTTTGA ACACCTTATG ACCGTCTATC TCTCAACCAA GGCTGCAACC 
ACACAGCCCA CCATGTTGCA GAACTGCCTC AACCTCCAGG CCTCCTGCGC CGCCCTGCTG 
GTTCAGCTCA GCCTGGGCAA TCAGGGCCCT GAACACATTC CTCTCACCTT CCCTTTACCT 
TCACTAGAGA ACAGCCTGCT TTGCTATGTG CCAGAGTTCT TTGCTGAAAA CATGGGTGAT 
TTCTTCATAT TCCTGCGCCG CTTTGCTGAT GAGGTGTTGG AGTCCTCTGC GGAAAGCTTA 
GAACACGTCC TGACCTTCAT CACTGTGCTC ATGGGCAATG TTGATAGAAT GAAGAATCCT 
CATTTGCGGG CAAAGTTGGC AGAGGTCCTT GAGGCTGTGA TGCCCCATAT GGAGACTCTG 
TCACCTGGCG CTGCCCAACC TATCATGTTT CAGCGCCAAA GGGTTTTCAG CTCATATCGG 
CACGCGCCCC AACTCGCTGA AGCCCTTATT ACAGTGTTCG TGGATATAGA GTTCACCGGT 
GATCCACATC AGTTTGAGCA GAAGTTTAAT TACAGGAGGC CAATGTACGC CATTCTGAAG 
TACATGTGGG GAGAGAAGAG TTACAGAGAG AGCATTAAGA ATCTTGCCGA CTATGCCTCT 
GAAAACCTTG AAGCCATGAA TCCACCACTC TTCCTTAGGT TTCTCAATCT CCTCATGAAT 
GATGCCATCT TCTTACTTGA CGAGGCTATA CAGTACTTAA GTAAGATCAA AATCCTCCAG 
CTGGAGCGGG ATCGGGGGGA GTGGGACAGT CTGGCCCCTG ACGCCCGCAG AGAGAAAGAA 
TCGAGTCTGC AGATGTTTGG ACAGCTGGGT CGCTTCCACA ACATCATGTC AAATGAGACC 
ATTGGCACCT TGGCTTTCCT CACATCTGAG ATAAAAGGTC TTTTTGTTCA CCCCTTTCTT 
GCTGAGAGGA TCATCTCCAT GCTCAACTAC TTTTTACAGC ACCTGGTAGG CCCCAAAATG 
GGCGCCTTGA AGGTTAAGGA CTTCAGTGAA TTTGACTTCA AACCCCAACA GCTTGTGTCG 
GACATCTGTA CTATTTACCT AAACTTGGGA GATGAAGAAA ATTTCTGCGC CACCGTCCCA 
AAAGATGGAA GGTCATATTC ACCCACTCTG TTCTGTCAAA CTGTCCGCGT CCTCAAGAAA 
ATCAACAAGC CTGGTGACAT GATTATATCT TTCAGTCTCC TAGCAGATAA AATTAAGTCT 
CTTGCAGACA GGCATCAGCA AGAGGAAGAG ACGTTCTCTG ATGCTCCAGA TGAATTTCTG 
GATCCCATTA TGTCTACCTT GATGCTCGAT CCTGTGCTTT TACCGTCTTC AAACGTCACA 
GTGGACCGCT CAACAATAGC ACGACACTTA CTAAGTGACC AGACAGACCC ATTCAACCGC 
AGTCCGCTAA CCATGGACCA GATCAGGCCC AACGAAGAGC TCAGGCAACA GATCATGAAG 
TGGCTTGCTG ATCATAAGCA AAGCAACCAG CAAATGGGAC CCAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290012.1
CDS	117..3347
Translation	MTDQGNNNQNISQNPFAALFSSVADAKQFASDQKQQRPTEQQSVDSGESQSESENSASESIDENDDSVAEISRSFRSRQELCEQLNVNHMIQRIFLITLDNSDPSLRSGNGIPPRCVYLEEMAADLDGQDWLNMDTIEQALFNRLLLQEPGNHLIYMTSCSVVNLSADRDAGEKRAIPYLYACYQRAKEEITKVPEKLLSFAVHCKNLTVSNARTVLLTPEIYISQNVYEQLLDLLLEAVRGAQFEEVVEFLEEVIASLLADQEVRTFGEVMVPVFDIFHGRVKDLDLCQLLLFSYLEILLYFSRQKDIAKVLMEHIQPKDPNNGIQYQKTLLGAILNISCLLKTPGVVENHGFFLNPSRSSPQELKVQESNIHQFMGQFHDKLYQILKNLLQQSGETRHLLLSWLGGCLQANMGRAKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFSRPYSPKLLTFNPTYCLLKELSEEERRNRNVHARGLDKETCLIPVPPQQTVEFAQSYSLLTENLILTQLTLYLGFHRLHDQMVKMNQSLHRLQGTWRDTQLSGGPAAAELREQFEHLMTVYLSTKAATTQPTMLQNCLNLQASCAALLVQLSLGNQGPEHIPLTFPLPSLENSLLCYVPEFFAENMGDFFIFLRRFADEVLESSAESLEHVLTFITVLMGNVDRMKNPHLRAKLAEVLEAVMPHMETLSPGAAQPIMFQRQRVFSSYRHAPQLAEALITVFVDIEFTGDPHQFEQKFNYRRPMYAILKYMWGEKSYRESIKNLADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKILQLERDRGEWDSLAPDARREKESSLQMFGQLGRFHNIMSNETIGTLAFLTSEIKGLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFCQTVRVLKKINKPGDMIISFSLLADKIKSLADRHQQEEETFSDAPDEFLDPIMSTLMLDPVLLPSSNVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNEELRQQIMKWLADHKQSNQQMGPSG

Target ORF information:

RefSeq Version	XM_009291737.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291737.2

ORF Insert Sequence:

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATCAG TAGATTCAGG AGAGAGCCAG TCTGAGTCAG AAAACTCTGC GTCTGAAAGC 
ATTGATGAGA ATGATGACTC AGTGGCGGAG ATCAGCCGAT CGTTCCGCTC ACGACAGGAG 
CTCTGTGAAC AGCTCAATGT CAACCACATG ATCCAGAGGA TCTTCCTCAT CACTCTGGAC 
AACAGTGATC CGAGTCTAAG AAGTGGCAAT GGCATCCCAC CACGCTGTGT TTACCTTGAG 
GAAATGGCAG CTGATCTTGA TGGTCAGGAC TGGCTTAACA TGGACACCAT AGAGCAGGCT 
TTGTTCAACA GGCTGCTTTT GCAGGAGCCA GGAAACCATC TCATTTACAT GACCTCCTGT 
AGTGTAGTGA ATCTCTCGGC AGATCGAGAC GCTGGAGAGA AACGTGCCAT ACCTTACCTT 
TACGCCTGCT ACCAAAGAGC TAAAGAGGAG ATCACTAAAG TCCCTGAGAA ACTGTTGTCA 
TTTGCTGTGC ACTGCAAAAA CCTGACCGTG TCCAATGCAC GAACTGTGCT GCTGACCCCA 
GAGATCTACA TCAGTCAGAA CGTTTACGAG CAGCTTCTGG ACCTGTTGCT GGAGGCTGTC 
AGAGGAGCCC AGTTTGAAGA GGTTGTGGAG TTTTTAGAGG AGGTCATTGC TAGCCTGTTG 
GCTGACCAAG AAGTGCGGAC CTTTGGGGAG GTAATGGTGC CAGTGTTTGA TATTTTCCAT 
GGCCGGGTTA AAGACCTAGA CCTCTGTCAG CTGCTGCTGT TTTCCTACCT GGAAATTCTC 
CTGTACTTCA GTCGACAAAA GGACATTGCT AAGGTTTTAA TGGAGCACAT TCAGCCCAAA 
GACCCTAACA ATGGGATTCA GTATCAGAAA ACACTTCTGG GAGCCATCCT TAATATTTCA 
TGTCTGCTGA AAACTCCTGG TGTGGTTGAA AACCATGGCT TTTTCCTGAA TCCATCTCGC 
TCCAGCCCAC AGGAATTGAA GGTTCAAGAG TCAAATATAC ACCAGTTTAT GGGGCAGTTT 
CATGATAAGC TCTATCAGAT CCTGAAAAAC TTGCTCCAGC AGTCCGGCGA GACTCGCCAC 
CTGCTGCTCT CGTGGCTGGG TGGCTGCCTG CAGGCCAACA TGGGTCGGGC CAAAATCTGG 
GCAAACCAGA TGCCCGAGAT ATTCTTCCAA ATGTATGCAT CGGATGCATT CTTCTTAAAT 
CTGGGAGCCG CTCTGCTCAA GCTCTGCCAG CCGTTTTCTC GACCCTACTC TCCTAAACTC 
CTGACCTTTA ACCCCACATA CTGCTTGCTT AAGGAGCTGA GCGAAGAAGA GCGGCGTAAC 
AGAAACGTGC ATGCTAGAGG TCTGGACAAG GAGACATGTC TGATACCTGT GCCCCCTCAA 
CAAACGGTTG AATTTGCCCA ATCATACAGC CTTCTGACAG AAAACCTCAT TCTTACACAA 
CTCACCCTGT ACCTTGGCTT CCATAGACTT CATGATCAGA TGGTGAAGAT GAATCAGTCT 
CTGCACCGGC TCCAGGGGAC GTGGCGGGAC ACGCAGCTCA GTGGAGGCCC AGCAGCAGCA 
GAGCTGCGGG AACAGTTTGA ACACCTTATG ACCGTCTATC TCTCAACCAA GGCTGCAACC 
ACACAGCCCA CCATGTTGCA GAACTGCCTC AACCTCCAGG CCTCCTGCGC CGCCCTGCTG 
GTTCAGCTCA GCCTGGGCAA TCAGGGCCCT GAACACATTC CTCTCACCTT CCCTTTACCT 
TCACTAGAGA ACAGCCTGCT TTGCTATGTG CCAGAGTTCT TTGCTGAAAA CATGGGTGAT 
TTCTTCATAT TCCTGCGCCG CTTTGCTGAT GAGGTGTTGG AGTCCTCTGC GGAAAGCTTA 
GAACACGTCC TGACCTTCAT CACTGTGCTC ATGGGCAATG TTGATAGAAT GAAGAATCCT 
CATTTGCGGG CAAAGTTGGC AGAGGTCCTT GAGGCTGTGA TGCCCCATAT GGAGACTCTG 
TCACCTGGCG CTGCCCAACC TATCATGTTT CAGCGCCAAA GGGTTTTCAG CTCATATCGG 
CACGCGCCCC AACTCGCTGA AGCCCTTATT ACAGTGTTCG TGGATATAGA GTTCACCGGT 
GATCCACATC AGTTTGAGCA GAAGTTTAAT TACAGGAGGC CAATGTACGC CATTCTGAAG 
TACATGTGGG GAGAGAAGAG TTACAGAGAG AGCATTAAGA ATCTTGCCGA CTATGCCTCT 
GAAAACCTTG AAGCCATGAA TCCACCACTC TTCCTTAGGT TTCTCAATCT CCTCATGAAT 
GATGCCATCT TCTTACTTGA CGAGGCTATA CAGTACTTAA GTAAGATCAA AATCCTCCAG 
CTGGAGCGGG ATCGGGGGGA GTGGGACAGT CTGGCCCCTG ACGCCCGCAG AGAGAAAGAA 
TCGAGTCTGC AGATGTTTGG ACAGCTGGGT CGCTTCCACA ACATCATGTC AAATGAGACC 
ATTGGCACCT TGGCTTTCCT CACATCTGAG ATAAAAGGTC TTTTTGTTCA CCCCTTTCTT 
GCTGAGAGGA TCATCTCCAT GCTCAACTAC TTTTTACAGC ACCTGGTAGG CCCCAAAATG 
GGCGCCTTGA AGGTTAAGGA CTTCAGTGAA TTTGACTTCA AACCCCAACA GCTTGTGTCG 
GACATCTGTA CTATTTACCT AAACTTGGGA GATGAAGAAA ATTTCTGCGC CACCGTCCCA 
AAAGATGGAA GGTCATATTC ACCCACTCTG TTCTGTCAAA CTGTCCGCGT CCTCAAGAAA 
ATCAACAAGC CTGGTGACAT GATTATATCT TTCAGTCTCC TAGCAGATAA AATTAAGTCT 
CTTGCAGACA GGCATCAGCA AGAGGAAGAG ACGTTCTCTG ATGCTCCAGA TGAATTTCTG 
GATCCCATTA TGTCTACCTT GATGCTCGAT CCTGTGCTTT TACCGTCTTC AAACGTCACA 
GTGGACCGCT CAACAATAGC ACGACACTTA CTAAGTGACC AGACAGACCC ATTCAACCGC 
AGTCCGCTAA CCATGGACCA GATCAGGCCC AACGAAGAGC TCAGGCAACA GATCATGAAG 
TGGCTTGCTG ATCATAAGCA AAGCAACCAG CAAATGGGAC CCAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_009291737.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290012.1 Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20491
Clone ID Related Accession (Same CDS sequence)	XM_009291737.2 , XM_009291737.3
Accession Version	XM_009291737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATCAG TAGATTCAGG AGAGAGCCAG TCTGAGTCAG AAAACTCTGC GTCTGAAAGC 
ATTGATGAGA ATGATGACTC AGTGGCGGAG ATCAGCCGAT CGTTCCGCTC ACGACAGGAG 
CTCTGTGAAC AGCTCAATGT CAACCACATG ATCCAGAGGA TCTTCCTCAT CACTCTGGAC 
AACAGTGATC CGAGTCTAAG AAGTGGCAAT GGCATCCCAC CACGCTGTGT TTACCTTGAG 
GAAATGGCAG CTGATCTTGA TGGTCAGGAC TGGCTTAACA TGGACACCAT AGAGCAGGCT 
TTGTTCAACA GGCTGCTTTT GCAGGAGCCA GGAAACCATC TCATTTACAT GACCTCCTGT 
AGTGTAGTGA ATCTCTCGGC AGATCGAGAC GCTGGAGAGA AACGTGCCAT ACCTTACCTT 
TACGCCTGCT ACCAAAGAGC TAAAGAGGAG ATCACTAAAG TCCCTGAGAA ACTGTTGTCA 
TTTGCTGTGC ACTGCAAAAA CCTGACCGTG TCCAATGCAC GAACTGTGCT GCTGACCCCA 
GAGATCTACA TCAGTCAGAA CGTTTACGAG CAGCTTCTGG ACCTGTTGCT GGAGGCTGTC 
AGAGGAGCCC AGTTTGAAGA GGTTGTGGAG TTTTTAGAGG AGGTCATTGC TAGCCTGTTG 
GCTGACCAAG AAGTGCGGAC CTTTGGGGAG GTAATGGTGC CAGTGTTTGA TATTTTCCAT 
GGCCGGGTTA AAGACCTAGA CCTCTGTCAG CTGCTGCTGT TTTCCTACCT GGAAATTCTC 
CTGTACTTCA GTCGACAAAA GGACATTGCT AAGGTTTTAA TGGAGCACAT TCAGCCCAAA 
GACCCTAACA ATGGGATTCA GTATCAGAAA ACACTTCTGG GAGCCATCCT TAATATTTCA 
TGTCTGCTGA AAACTCCTGG TGTGGTTGAA AACCATGGCT TTTTCCTGAA TCCATCTCGC 
TCCAGCCCAC AGGAATTGAA GGTTCAAGAG TCAAATATAC ACCAGTTTAT GGGGCAGTTT 
CATGATAAGC TCTATCAGAT CCTGAAAAAC TTGCTCCAGC AGTCCGGCGA GACTCGCCAC 
CTGCTGCTCT CGTGGCTGGG TGGCTGCCTG CAGGCCAACA TGGGTCGGGC CAAAATCTGG 
GCAAACCAGA TGCCCGAGAT ATTCTTCCAA ATGTATGCAT CGGATGCATT CTTCTTAAAT 
CTGGGAGCCG CTCTGCTCAA GCTCTGCCAG CCGTTTTCTC GACCCTACTC TCCTAAACTC 
CTGACCTTTA ACCCCACATA CTGCTTGCTT AAGGAGCTGA GCGAAGAAGA GCGGCGTAAC 
AGAAACGTGC ATGCTAGAGG TCTGGACAAG GAGACATGTC TGATACCTGT GCCCCCTCAA 
CAAACGGTTG AATTTGCCCA ATCATACAGC CTTCTGACAG AAAACCTCAT TCTTACACAA 
CTCACCCTGT ACCTTGGCTT CCATAGACTT CATGATCAGA TGGTGAAGAT GAATCAGTCT 
CTGCACCGGC TCCAGGGGAC GTGGCGGGAC ACGCAGCTCA GTGGAGGCCC AGCAGCAGCA 
GAGCTGCGGG AACAGTTTGA ACACCTTATG ACCGTCTATC TCTCAACCAA GGCTGCAACC 
ACACAGCCCA CCATGTTGCA GAACTGCCTC AACCTCCAGG CCTCCTGCGC CGCCCTGCTG 
GTTCAGCTCA GCCTGGGCAA TCAGGGCCCT GAACACATTC CTCTCACCTT CCCTTTACCT 
TCACTAGAGA ACAGCCTGCT TTGCTATGTG CCAGAGTTCT TTGCTGAAAA CATGGGTGAT 
TTCTTCATAT TCCTGCGCCG CTTTGCTGAT GAGGTGTTGG AGTCCTCTGC GGAAAGCTTA 
GAACACGTCC TGACCTTCAT CACTGTGCTC ATGGGCAATG TTGATAGAAT GAAGAATCCT 
CATTTGCGGG CAAAGTTGGC AGAGGTCCTT GAGGCTGTGA TGCCCCATAT GGAGACTCTG 
TCACCTGGCG CTGCCCAACC TATCATGTTT CAGCGCCAAA GGGTTTTCAG CTCATATCGG 
CACGCGCCCC AACTCGCTGA AGCCCTTATT ACAGTGTTCG TGGATATAGA GTTCACCGGT 
GATCCACATC AGTTTGAGCA GAAGTTTAAT TACAGGAGGC CAATGTACGC CATTCTGAAG 
TACATGTGGG GAGAGAAGAG TTACAGAGAG AGCATTAAGA ATCTTGCCGA CTATGCCTCT 
GAAAACCTTG AAGCCATGAA TCCACCACTC TTCCTTAGGT TTCTCAATCT CCTCATGAAT 
GATGCCATCT TCTTACTTGA CGAGGCTATA CAGTACTTAA GTAAGATCAA AATCCTCCAG 
CTGGAGCGGG ATCGGGGGGA GTGGGACAGT CTGGCCCCTG ACGCCCGCAG AGAGAAAGAA 
TCGAGTCTGC AGATGTTTGG ACAGCTGGGT CGCTTCCACA ACATCATGTC AAATGAGACC 
ATTGGCACCT TGGCTTTCCT CACATCTGAG ATAAAAGGTC TTTTTGTTCA CCCCTTTCTT 
GCTGAGAGGA TCATCTCCAT GCTCAACTAC TTTTTACAGC ACCTGGTAGG CCCCAAAATG 
GGCGCCTTGA AGGTTAAGGA CTTCAGTGAA TTTGACTTCA AACCCCAACA GCTTGTGTCG 
GACATCTGTA CTATTTACCT AAACTTGGGA GATGAAGAAA ATTTCTGCGC CACCGTCCCA 
AAAGATGGAA GGTCATATTC ACCCACTCTG TTCTGTCAAA CTGTCCGCGT CCTCAAGAAA 
ATCAACAAGC CTGGTGACAT GATTATATCT TTCAGTCTCC TAGCAGATAA AATTAAGTCT 
CTTGCAGACA GGCATCAGCA AGAGGAAGAG ACGTTCTCTG ATGCTCCAGA TGAATTTCTG 
GATCCCATTA TGTCTACCTT GATGCTCGAT CCTGTGCTTT TACCGTCTTC AAACGTCACA 
GTGGACCGCT CAACAATAGC ACGACACTTA CTAAGTGACC AGACAGACCC ATTCAACCGC 
AGTCCGCTAA CCATGGACCA GATCAGGCCC AACGAAGAGC TCAGGCAACA GATCATGAAG 
TGGCTTGCTG ATCATAAGCA AAGCAACCAG CAAATGGGAC CCAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290012.1
CDS	124..3354
Translation	MTDQGNNNQNISQNPFAALFSSVADAKQFASDQKQQRPTEQQSVDSGESQSESENSASESIDENDDSVAEISRSFRSRQELCEQLNVNHMIQRIFLITLDNSDPSLRSGNGIPPRCVYLEEMAADLDGQDWLNMDTIEQALFNRLLLQEPGNHLIYMTSCSVVNLSADRDAGEKRAIPYLYACYQRAKEEITKVPEKLLSFAVHCKNLTVSNARTVLLTPEIYISQNVYEQLLDLLLEAVRGAQFEEVVEFLEEVIASLLADQEVRTFGEVMVPVFDIFHGRVKDLDLCQLLLFSYLEILLYFSRQKDIAKVLMEHIQPKDPNNGIQYQKTLLGAILNISCLLKTPGVVENHGFFLNPSRSSPQELKVQESNIHQFMGQFHDKLYQILKNLLQQSGETRHLLLSWLGGCLQANMGRAKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFSRPYSPKLLTFNPTYCLLKELSEEERRNRNVHARGLDKETCLIPVPPQQTVEFAQSYSLLTENLILTQLTLYLGFHRLHDQMVKMNQSLHRLQGTWRDTQLSGGPAAAELREQFEHLMTVYLSTKAATTQPTMLQNCLNLQASCAALLVQLSLGNQGPEHIPLTFPLPSLENSLLCYVPEFFAENMGDFFIFLRRFADEVLESSAESLEHVLTFITVLMGNVDRMKNPHLRAKLAEVLEAVMPHMETLSPGAAQPIMFQRQRVFSSYRHAPQLAEALITVFVDIEFTGDPHQFEQKFNYRRPMYAILKYMWGEKSYRESIKNLADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKILQLERDRGEWDSLAPDARREKESSLQMFGQLGRFHNIMSNETIGTLAFLTSEIKGLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFCQTVRVLKKINKPGDMIISFSLLADKIKSLADRHQQEEETFSDAPDEFLDPIMSTLMLDPVLLPSSNVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNEELRQQIMKWLADHKQSNQQMGPSG

Target ORF information:

RefSeq Version	XM_009291737.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291737.3

ORF Insert Sequence:

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATCAG TAGATTCAGG AGAGAGCCAG TCTGAGTCAG AAAACTCTGC GTCTGAAAGC 
ATTGATGAGA ATGATGACTC AGTGGCGGAG ATCAGCCGAT CGTTCCGCTC ACGACAGGAG 
CTCTGTGAAC AGCTCAATGT CAACCACATG ATCCAGAGGA TCTTCCTCAT CACTCTGGAC 
AACAGTGATC CGAGTCTAAG AAGTGGCAAT GGCATCCCAC CACGCTGTGT TTACCTTGAG 
GAAATGGCAG CTGATCTTGA TGGTCAGGAC TGGCTTAACA TGGACACCAT AGAGCAGGCT 
TTGTTCAACA GGCTGCTTTT GCAGGAGCCA GGAAACCATC TCATTTACAT GACCTCCTGT 
AGTGTAGTGA ATCTCTCGGC AGATCGAGAC GCTGGAGAGA AACGTGCCAT ACCTTACCTT 
TACGCCTGCT ACCAAAGAGC TAAAGAGGAG ATCACTAAAG TCCCTGAGAA ACTGTTGTCA 
TTTGCTGTGC ACTGCAAAAA CCTGACCGTG TCCAATGCAC GAACTGTGCT GCTGACCCCA 
GAGATCTACA TCAGTCAGAA CGTTTACGAG CAGCTTCTGG ACCTGTTGCT GGAGGCTGTC 
AGAGGAGCCC AGTTTGAAGA GGTTGTGGAG TTTTTAGAGG AGGTCATTGC TAGCCTGTTG 
GCTGACCAAG AAGTGCGGAC CTTTGGGGAG GTAATGGTGC CAGTGTTTGA TATTTTCCAT 
GGCCGGGTTA AAGACCTAGA CCTCTGTCAG CTGCTGCTGT TTTCCTACCT GGAAATTCTC 
CTGTACTTCA GTCGACAAAA GGACATTGCT AAGGTTTTAA TGGAGCACAT TCAGCCCAAA 
GACCCTAACA ATGGGATTCA GTATCAGAAA ACACTTCTGG GAGCCATCCT TAATATTTCA 
TGTCTGCTGA AAACTCCTGG TGTGGTTGAA AACCATGGCT TTTTCCTGAA TCCATCTCGC 
TCCAGCCCAC AGGAATTGAA GGTTCAAGAG TCAAATATAC ACCAGTTTAT GGGGCAGTTT 
CATGATAAGC TCTATCAGAT CCTGAAAAAC TTGCTCCAGC AGTCCGGCGA GACTCGCCAC 
CTGCTGCTCT CGTGGCTGGG TGGCTGCCTG CAGGCCAACA TGGGTCGGGC CAAAATCTGG 
GCAAACCAGA TGCCCGAGAT ATTCTTCCAA ATGTATGCAT CGGATGCATT CTTCTTAAAT 
CTGGGAGCCG CTCTGCTCAA GCTCTGCCAG CCGTTTTCTC GACCCTACTC TCCTAAACTC 
CTGACCTTTA ACCCCACATA CTGCTTGCTT AAGGAGCTGA GCGAAGAAGA GCGGCGTAAC 
AGAAACGTGC ATGCTAGAGG TCTGGACAAG GAGACATGTC TGATACCTGT GCCCCCTCAA 
CAAACGGTTG AATTTGCCCA ATCATACAGC CTTCTGACAG AAAACCTCAT TCTTACACAA 
CTCACCCTGT ACCTTGGCTT CCATAGACTT CATGATCAGA TGGTGAAGAT GAATCAGTCT 
CTGCACCGGC TCCAGGGGAC GTGGCGGGAC ACGCAGCTCA GTGGAGGCCC AGCAGCAGCA 
GAGCTGCGGG AACAGTTTGA ACACCTTATG ACCGTCTATC TCTCAACCAA GGCTGCAACC 
ACACAGCCCA CCATGTTGCA GAACTGCCTC AACCTCCAGG CCTCCTGCGC CGCCCTGCTG 
GTTCAGCTCA GCCTGGGCAA TCAGGGCCCT GAACACATTC CTCTCACCTT CCCTTTACCT 
TCACTAGAGA ACAGCCTGCT TTGCTATGTG CCAGAGTTCT TTGCTGAAAA CATGGGTGAT 
TTCTTCATAT TCCTGCGCCG CTTTGCTGAT GAGGTGTTGG AGTCCTCTGC GGAAAGCTTA 
GAACACGTCC TGACCTTCAT CACTGTGCTC ATGGGCAATG TTGATAGAAT GAAGAATCCT 
CATTTGCGGG CAAAGTTGGC AGAGGTCCTT GAGGCTGTGA TGCCCCATAT GGAGACTCTG 
TCACCTGGCG CTGCCCAACC TATCATGTTT CAGCGCCAAA GGGTTTTCAG CTCATATCGG 
CACGCGCCCC AACTCGCTGA AGCCCTTATT ACAGTGTTCG TGGATATAGA GTTCACCGGT 
GATCCACATC AGTTTGAGCA GAAGTTTAAT TACAGGAGGC CAATGTACGC CATTCTGAAG 
TACATGTGGG GAGAGAAGAG TTACAGAGAG AGCATTAAGA ATCTTGCCGA CTATGCCTCT 
GAAAACCTTG AAGCCATGAA TCCACCACTC TTCCTTAGGT TTCTCAATCT CCTCATGAAT 
GATGCCATCT TCTTACTTGA CGAGGCTATA CAGTACTTAA GTAAGATCAA AATCCTCCAG 
CTGGAGCGGG ATCGGGGGGA GTGGGACAGT CTGGCCCCTG ACGCCCGCAG AGAGAAAGAA 
TCGAGTCTGC AGATGTTTGG ACAGCTGGGT CGCTTCCACA ACATCATGTC AAATGAGACC 
ATTGGCACCT TGGCTTTCCT CACATCTGAG ATAAAAGGTC TTTTTGTTCA CCCCTTTCTT 
GCTGAGAGGA TCATCTCCAT GCTCAACTAC TTTTTACAGC ACCTGGTAGG CCCCAAAATG 
GGCGCCTTGA AGGTTAAGGA CTTCAGTGAA TTTGACTTCA AACCCCAACA GCTTGTGTCG 
GACATCTGTA CTATTTACCT AAACTTGGGA GATGAAGAAA ATTTCTGCGC CACCGTCCCA 
AAAGATGGAA GGTCATATTC ACCCACTCTG TTCTGTCAAA CTGTCCGCGT CCTCAAGAAA 
ATCAACAAGC CTGGTGACAT GATTATATCT TTCAGTCTCC TAGCAGATAA AATTAAGTCT 
CTTGCAGACA GGCATCAGCA AGAGGAAGAG ACGTTCTCTG ATGCTCCAGA TGAATTTCTG 
GATCCCATTA TGTCTACCTT GATGCTCGAT CCTGTGCTTT TACCGTCTTC AAACGTCACA 
GTGGACCGCT CAACAATAGC ACGACACTTA CTAAGTGACC AGACAGACCC ATTCAACCGC 
AGTCCGCTAA CCATGGACCA GATCAGGCCC AACGAAGAGC TCAGGCAACA GATCATGAAG 
TGGCTTGCTG ATCATAAGCA AAGCAACCAG CAAATGGGAC CCAGTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_003200047.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200095.2 Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20492
Clone ID Related Accession (Same CDS sequence)	XM_003200047.5 , XM_003200047.4
Accession Version	XM_003200047.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200047.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATTAG ATTCAGGAGA GAGCCAGTCT GAGTCAGAAA ACTCTGCGTC TGAAAGCATT 
GATGAGAATG ATGACTCAGT GGCGGAGATC AGCCGATCGT TCCGCTCACG ACAGGAGCTC 
TGTGAACAGC TCAATGTCAA CCACATGATC CAGAGGATCT TCCTCATCAC TCTGGACAAC 
AGTGATCCGA GTCTAAGAAG TGGCAATGGC ATCCCACCAC GCTGTGTTTA CCTTGAGGAA 
ATGGCAGCTG ATCTTGATGG TCAGGACTGG CTTAACATGG ACACCATAGA GCAGGCTTTG 
TTCAACAGGC TGCTTTTGCA GGAGCCAGGA AACCATCTCA TTTACATGAC CTCCTGTAGT 
GTAGTGAATC TCTCGGCAGA TCGAGACGCT GGAGAGAAAC GTGCCATACC TTACCTTTAC 
GCCTGCTACC AAAGAGCTAA AGAGGAGATC ACTAAAGTCC CTGAGAAACT GTTGTCATTT 
GCTGTGCACT GCAAAAACCT GACCGTGTCC AATGCACGAA CTGTGCTGCT GACCCCAGAG 
ATCTACATCA GTCAGAACGT TTACGAGCAG CTTCTGGACC TGTTGCTGGA GGCTGTCAGA 
GGAGCCCAGT TTGAAGAGGT TGTGGAGTTT TTAGAGGAGG TCATTGCTAG CCTGTTGGCT 
GACCAAGAAG TGCGGACCTT TGGGGAGGTA ATGGTGCCAG TGTTTGATAT TTTCCATGGC 
CGGGTTAAAG ACCTAGACCT CTGTCAGCTG CTGCTGTTTT CCTACCTGGA AATTCTCCTG 
TACTTCAGTC GACAAAAGGA CATTGCTAAG GTTTTAATGG AGCACATTCA GCCCAAAGAC 
CCTAACAATG GGATTCAGTA TCAGAAAACA CTTCTGGGAG CCATCCTTAA TATTTCATGT 
CTGCTGAAAA CTCCTGGTGT GGTTGAAAAC CATGGCTTTT TCCTGAATCC ATCTCGCTCC 
AGCCCACAGG AATTGAAGGT TCAAGAGTCA AATATACACC AGTTTATGGG GCAGTTTCAT 
GATAAGCTCT ATCAGATCCT GAAAAACTTG CTCCAGCAGT CCGGCGAGAC TCGCCACCTG 
CTGCTCTCGT GGCTGGGTGG CTGCCTGCAG GCCAACATGG GTCGGGCCAA AATCTGGGCA 
AACCAGATGC CCGAGATATT CTTCCAAATG TATGCATCGG ATGCATTCTT CTTAAATCTG 
GGAGCCGCTC TGCTCAAGCT CTGCCAGCCG TTTTCTCGAC CCTACTCTCC TAAACTCCTG 
ACCTTTAACC CCACATACTG CTTGCTTAAG GAGCTGAGCG AAGAAGAGCG GCGTAACAGA 
AACGTGCATG CTAGAGGTCT GGACAAGGAG ACATGTCTGA TACCTGTGCC CCCTCAACAA 
ACGGTTGAAT TTGCCCAATC ATACAGCCTT CTGACAGAAA ACCTCATTCT TACACAACTC 
ACCCTGTACC TTGGCTTCCA TAGACTTCAT GATCAGATGG TGAAGATGAA TCAGTCTCTG 
CACCGGCTCC AGGGGACGTG GCGGGACACG CAGCTCAGTG GAGGCCCAGC AGCAGCAGAG 
CTGCGGGAAC AGTTTGAACA CCTTATGACC GTCTATCTCT CAACCAAGGC TGCAACCACA 
CAGCCCACCA TGTTGCAGAA CTGCCTCAAC CTCCAGGCCT CCTGCGCCGC CCTGCTGGTT 
CAGCTCAGCC TGGGCAATCA GGGCCCTGAA CACATTCCTC TCACCTTCCC TTTACCTTCA 
CTAGAGAACA GCCTGCTTTG CTATGTGCCA GAGTTCTTTG CTGAAAACAT GGGTGATTTC 
TTCATATTCC TGCGCCGCTT TGCTGATGAG GTGTTGGAGT CCTCTGCGGA AAGCTTAGAA 
CACGTCCTGA CCTTCATCAC TGTGCTCATG GGCAATGTTG ATAGAATGAA GAATCCTCAT 
TTGCGGGCAA AGTTGGCAGA GGTCCTTGAG GCTGTGATGC CCCATATGGA GACTCTGTCA 
CCTGGCGCTG CCCAACCTAT CATGTTTCAG CGCCAAAGGG TTTTCAGCTC ATATCGGCAC 
GCGCCCCAAC TCGCTGAAGC CCTTATTACA GTGTTCGTGG ATATAGAGTT CACCGGTGAT 
CCACATCAGT TTGAGCAGAA GTTTAATTAC AGGAGGCCAA TGTACGCCAT TCTGAAGTAC 
ATGTGGGGAG AGAAGAGTTA CAGAGAGAGC ATTAAGAATC TTGCCGACTA TGCCTCTGAA 
AACCTTGAAG CCATGAATCC ACCACTCTTC CTTAGGTTTC TCAATCTCCT CATGAATGAT 
GCCATCTTCT TACTTGACGA GGCTATACAG TACTTAAGTA AGATCAAAAT CCTCCAGCTG 
GAGCGGGATC GGGGGGAGTG GGACAGTCTG GCCCCTGACG CCCGCAGAGA GAAAGAATCG 
AGTCTGCAGA TGTTTGGACA GCTGGGTCGC TTCCACAACA TCATGTCAAA TGAGACCATT 
GGCACCTTGG CTTTCCTCAC ATCTGAGATA AAAGGTCTTT TTGTTCACCC CTTTCTTGCT 
GAGAGGATCA TCTCCATGCT CAACTACTTT TTACAGCACC TGGTAGGCCC CAAAATGGGC 
GCCTTGAAGG TTAAGGACTT CAGTGAATTT GACTTCAAAC CCCAACAGCT TGTGTCGGAC 
ATCTGTACTA TTTACCTAAA CTTGGGAGAT GAAGAAAATT TCTGCGCCAC CGTCCCAAAA 
GATGGAAGGT CATATTCACC CACTCTGTTC TGTCAAACTG TCCGCGTCCT CAAGAAAATC 
AACAAGCCTG GTGACATGAT TATATCTTTC AGTCTCCTAG CAGATAAAAT TAAGTCTCTT 
GCAGACAGGC ATCAGCAAGA GGAAGAGACG TTCTCTGATG CTCCAGATGA ATTTCTGGAT 
CCCATTATGT CTACCTTGAT GCTCGATCCT GTGCTTTTAC CGTCTTCAAA CGTCACAGTG 
GACCGCTCAA CAATAGCACG ACACTTACTA AGTGACCAGA CAGACCCATT CAACCGCAGT 
CCGCTAACCA TGGACCAGAT CAGGCCCAAC GAAGAGCTCA GGCAACAGAT CATGAAGTGG 
CTTGCTGATC ATAAGCAAAG CAACCAGCAA ATGGGACCCA GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200095.2
CDS	124..3351
Translation	MTDQGNNNQNISQNPFAALFSSVADAKQFASDQKQQRPTEQQLDSGESQSESENSASESIDENDDSVAEISRSFRSRQELCEQLNVNHMIQRIFLITLDNSDPSLRSGNGIPPRCVYLEEMAADLDGQDWLNMDTIEQALFNRLLLQEPGNHLIYMTSCSVVNLSADRDAGEKRAIPYLYACYQRAKEEITKVPEKLLSFAVHCKNLTVSNARTVLLTPEIYISQNVYEQLLDLLLEAVRGAQFEEVVEFLEEVIASLLADQEVRTFGEVMVPVFDIFHGRVKDLDLCQLLLFSYLEILLYFSRQKDIAKVLMEHIQPKDPNNGIQYQKTLLGAILNISCLLKTPGVVENHGFFLNPSRSSPQELKVQESNIHQFMGQFHDKLYQILKNLLQQSGETRHLLLSWLGGCLQANMGRAKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFSRPYSPKLLTFNPTYCLLKELSEEERRNRNVHARGLDKETCLIPVPPQQTVEFAQSYSLLTENLILTQLTLYLGFHRLHDQMVKMNQSLHRLQGTWRDTQLSGGPAAAELREQFEHLMTVYLSTKAATTQPTMLQNCLNLQASCAALLVQLSLGNQGPEHIPLTFPLPSLENSLLCYVPEFFAENMGDFFIFLRRFADEVLESSAESLEHVLTFITVLMGNVDRMKNPHLRAKLAEVLEAVMPHMETLSPGAAQPIMFQRQRVFSSYRHAPQLAEALITVFVDIEFTGDPHQFEQKFNYRRPMYAILKYMWGEKSYRESIKNLADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKILQLERDRGEWDSLAPDARREKESSLQMFGQLGRFHNIMSNETIGTLAFLTSEIKGLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFCQTVRVLKKINKPGDMIISFSLLADKIKSLADRHQQEEETFSDAPDEFLDPIMSTLMLDPVLLPSSNVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNEELRQQIMKWLADHKQSNQQMGPSG

Target ORF information:

RefSeq Version	XM_003200047.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200047.5

ORF Insert Sequence:

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATTAG ATTCAGGAGA GAGCCAGTCT GAGTCAGAAA ACTCTGCGTC TGAAAGCATT 
GATGAGAATG ATGACTCAGT GGCGGAGATC AGCCGATCGT TCCGCTCACG ACAGGAGCTC 
TGTGAACAGC TCAATGTCAA CCACATGATC CAGAGGATCT TCCTCATCAC TCTGGACAAC 
AGTGATCCGA GTCTAAGAAG TGGCAATGGC ATCCCACCAC GCTGTGTTTA CCTTGAGGAA 
ATGGCAGCTG ATCTTGATGG TCAGGACTGG CTTAACATGG ACACCATAGA GCAGGCTTTG 
TTCAACAGGC TGCTTTTGCA GGAGCCAGGA AACCATCTCA TTTACATGAC CTCCTGTAGT 
GTAGTGAATC TCTCGGCAGA TCGAGACGCT GGAGAGAAAC GTGCCATACC TTACCTTTAC 
GCCTGCTACC AAAGAGCTAA AGAGGAGATC ACTAAAGTCC CTGAGAAACT GTTGTCATTT 
GCTGTGCACT GCAAAAACCT GACCGTGTCC AATGCACGAA CTGTGCTGCT GACCCCAGAG 
ATCTACATCA GTCAGAACGT TTACGAGCAG CTTCTGGACC TGTTGCTGGA GGCTGTCAGA 
GGAGCCCAGT TTGAAGAGGT TGTGGAGTTT TTAGAGGAGG TCATTGCTAG CCTGTTGGCT 
GACCAAGAAG TGCGGACCTT TGGGGAGGTA ATGGTGCCAG TGTTTGATAT TTTCCATGGC 
CGGGTTAAAG ACCTAGACCT CTGTCAGCTG CTGCTGTTTT CCTACCTGGA AATTCTCCTG 
TACTTCAGTC GACAAAAGGA CATTGCTAAG GTTTTAATGG AGCACATTCA GCCCAAAGAC 
CCTAACAATG GGATTCAGTA TCAGAAAACA CTTCTGGGAG CCATCCTTAA TATTTCATGT 
CTGCTGAAAA CTCCTGGTGT GGTTGAAAAC CATGGCTTTT TCCTGAATCC ATCTCGCTCC 
AGCCCACAGG AATTGAAGGT TCAAGAGTCA AATATACACC AGTTTATGGG GCAGTTTCAT 
GATAAGCTCT ATCAGATCCT GAAAAACTTG CTCCAGCAGT CCGGCGAGAC TCGCCACCTG 
CTGCTCTCGT GGCTGGGTGG CTGCCTGCAG GCCAACATGG GTCGGGCCAA AATCTGGGCA 
AACCAGATGC CCGAGATATT CTTCCAAATG TATGCATCGG ATGCATTCTT CTTAAATCTG 
GGAGCCGCTC TGCTCAAGCT CTGCCAGCCG TTTTCTCGAC CCTACTCTCC TAAACTCCTG 
ACCTTTAACC CCACATACTG CTTGCTTAAG GAGCTGAGCG AAGAAGAGCG GCGTAACAGA 
AACGTGCATG CTAGAGGTCT GGACAAGGAG ACATGTCTGA TACCTGTGCC CCCTCAACAA 
ACGGTTGAAT TTGCCCAATC ATACAGCCTT CTGACAGAAA ACCTCATTCT TACACAACTC 
ACCCTGTACC TTGGCTTCCA TAGACTTCAT GATCAGATGG TGAAGATGAA TCAGTCTCTG 
CACCGGCTCC AGGGGACGTG GCGGGACACG CAGCTCAGTG GAGGCCCAGC AGCAGCAGAG 
CTGCGGGAAC AGTTTGAACA CCTTATGACC GTCTATCTCT CAACCAAGGC TGCAACCACA 
CAGCCCACCA TGTTGCAGAA CTGCCTCAAC CTCCAGGCCT CCTGCGCCGC CCTGCTGGTT 
CAGCTCAGCC TGGGCAATCA GGGCCCTGAA CACATTCCTC TCACCTTCCC TTTACCTTCA 
CTAGAGAACA GCCTGCTTTG CTATGTGCCA GAGTTCTTTG CTGAAAACAT GGGTGATTTC 
TTCATATTCC TGCGCCGCTT TGCTGATGAG GTGTTGGAGT CCTCTGCGGA AAGCTTAGAA 
CACGTCCTGA CCTTCATCAC TGTGCTCATG GGCAATGTTG ATAGAATGAA GAATCCTCAT 
TTGCGGGCAA AGTTGGCAGA GGTCCTTGAG GCTGTGATGC CCCATATGGA GACTCTGTCA 
CCTGGCGCTG CCCAACCTAT CATGTTTCAG CGCCAAAGGG TTTTCAGCTC ATATCGGCAC 
GCGCCCCAAC TCGCTGAAGC CCTTATTACA GTGTTCGTGG ATATAGAGTT CACCGGTGAT 
CCACATCAGT TTGAGCAGAA GTTTAATTAC AGGAGGCCAA TGTACGCCAT TCTGAAGTAC 
ATGTGGGGAG AGAAGAGTTA CAGAGAGAGC ATTAAGAATC TTGCCGACTA TGCCTCTGAA 
AACCTTGAAG CCATGAATCC ACCACTCTTC CTTAGGTTTC TCAATCTCCT CATGAATGAT 
GCCATCTTCT TACTTGACGA GGCTATACAG TACTTAAGTA AGATCAAAAT CCTCCAGCTG 
GAGCGGGATC GGGGGGAGTG GGACAGTCTG GCCCCTGACG CCCGCAGAGA GAAAGAATCG 
AGTCTGCAGA TGTTTGGACA GCTGGGTCGC TTCCACAACA TCATGTCAAA TGAGACCATT 
GGCACCTTGG CTTTCCTCAC ATCTGAGATA AAAGGTCTTT TTGTTCACCC CTTTCTTGCT 
GAGAGGATCA TCTCCATGCT CAACTACTTT TTACAGCACC TGGTAGGCCC CAAAATGGGC 
GCCTTGAAGG TTAAGGACTT CAGTGAATTT GACTTCAAAC CCCAACAGCT TGTGTCGGAC 
ATCTGTACTA TTTACCTAAA CTTGGGAGAT GAAGAAAATT TCTGCGCCAC CGTCCCAAAA 
GATGGAAGGT CATATTCACC CACTCTGTTC TGTCAAACTG TCCGCGTCCT CAAGAAAATC 
AACAAGCCTG GTGACATGAT TATATCTTTC AGTCTCCTAG CAGATAAAAT TAAGTCTCTT 
GCAGACAGGC ATCAGCAAGA GGAAGAGACG TTCTCTGATG CTCCAGATGA ATTTCTGGAT 
CCCATTATGT CTACCTTGAT GCTCGATCCT GTGCTTTTAC CGTCTTCAAA CGTCACAGTG 
GACCGCTCAA CAATAGCACG ACACTTACTA AGTGACCAGA CAGACCCATT CAACCGCAGT 
CCGCTAACCA TGGACCAGAT CAGGCCCAAC GAAGAGCTCA GGCAACAGAT CATGAAGTGG 
CTTGCTGATC ATAAGCAAAG CAACCAGCAA ATGGGACCCA GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4a ( XM_003200047.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200095.2 Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20492
Clone ID Related Accession (Same CDS sequence)	XM_003200047.5 , XM_003200047.4
Accession Version	XM_003200047.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200047.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATTAG ATTCAGGAGA GAGCCAGTCT GAGTCAGAAA ACTCTGCGTC TGAAAGCATT 
GATGAGAATG ATGACTCAGT GGCGGAGATC AGCCGATCGT TCCGCTCACG ACAGGAGCTC 
TGTGAACAGC TCAATGTCAA CCACATGATC CAGAGGATCT TCCTCATCAC TCTGGACAAC 
AGTGATCCGA GTCTAAGAAG TGGCAATGGC ATCCCACCAC GCTGTGTTTA CCTTGAGGAA 
ATGGCAGCTG ATCTTGATGG TCAGGACTGG CTTAACATGG ACACCATAGA GCAGGCTTTG 
TTCAACAGGC TGCTTTTGCA GGAGCCAGGA AACCATCTCA TTTACATGAC CTCCTGTAGT 
GTAGTGAATC TCTCGGCAGA TCGAGACGCT GGAGAGAAAC GTGCCATACC TTACCTTTAC 
GCCTGCTACC AAAGAGCTAA AGAGGAGATC ACTAAAGTCC CTGAGAAACT GTTGTCATTT 
GCTGTGCACT GCAAAAACCT GACCGTGTCC AATGCACGAA CTGTGCTGCT GACCCCAGAG 
ATCTACATCA GTCAGAACGT TTACGAGCAG CTTCTGGACC TGTTGCTGGA GGCTGTCAGA 
GGAGCCCAGT TTGAAGAGGT TGTGGAGTTT TTAGAGGAGG TCATTGCTAG CCTGTTGGCT 
GACCAAGAAG TGCGGACCTT TGGGGAGGTA ATGGTGCCAG TGTTTGATAT TTTCCATGGC 
CGGGTTAAAG ACCTAGACCT CTGTCAGCTG CTGCTGTTTT CCTACCTGGA AATTCTCCTG 
TACTTCAGTC GACAAAAGGA CATTGCTAAG GTTTTAATGG AGCACATTCA GCCCAAAGAC 
CCTAACAATG GGATTCAGTA TCAGAAAACA CTTCTGGGAG CCATCCTTAA TATTTCATGT 
CTGCTGAAAA CTCCTGGTGT GGTTGAAAAC CATGGCTTTT TCCTGAATCC ATCTCGCTCC 
AGCCCACAGG AATTGAAGGT TCAAGAGTCA AATATACACC AGTTTATGGG GCAGTTTCAT 
GATAAGCTCT ATCAGATCCT GAAAAACTTG CTCCAGCAGT CCGGCGAGAC TCGCCACCTG 
CTGCTCTCGT GGCTGGGTGG CTGCCTGCAG GCCAACATGG GTCGGGCCAA AATCTGGGCA 
AACCAGATGC CCGAGATATT CTTCCAAATG TATGCATCGG ATGCATTCTT CTTAAATCTG 
GGAGCCGCTC TGCTCAAGCT CTGCCAGCCG TTTTCTCGAC CCTACTCTCC TAAACTCCTG 
ACCTTTAACC CCACATACTG CTTGCTTAAG GAGCTGAGCG AAGAAGAGCG GCGTAACAGA 
AACGTGCATG CTAGAGGTCT GGACAAGGAG ACATGTCTGA TACCTGTGCC CCCTCAACAA 
ACGGTTGAAT TTGCCCAATC ATACAGCCTT CTGACAGAAA ACCTCATTCT TACACAACTC 
ACCCTGTACC TTGGCTTCCA TAGACTTCAT GATCAGATGG TGAAGATGAA TCAGTCTCTG 
CACCGGCTCC AGGGGACGTG GCGGGACACG CAGCTCAGTG GAGGCCCAGC AGCAGCAGAG 
CTGCGGGAAC AGTTTGAACA CCTTATGACC GTCTATCTCT CAACCAAGGC TGCAACCACA 
CAGCCCACCA TGTTGCAGAA CTGCCTCAAC CTCCAGGCCT CCTGCGCCGC CCTGCTGGTT 
CAGCTCAGCC TGGGCAATCA GGGCCCTGAA CACATTCCTC TCACCTTCCC TTTACCTTCA 
CTAGAGAACA GCCTGCTTTG CTATGTGCCA GAGTTCTTTG CTGAAAACAT GGGTGATTTC 
TTCATATTCC TGCGCCGCTT TGCTGATGAG GTGTTGGAGT CCTCTGCGGA AAGCTTAGAA 
CACGTCCTGA CCTTCATCAC TGTGCTCATG GGCAATGTTG ATAGAATGAA GAATCCTCAT 
TTGCGGGCAA AGTTGGCAGA GGTCCTTGAG GCTGTGATGC CCCATATGGA GACTCTGTCA 
CCTGGCGCTG CCCAACCTAT CATGTTTCAG CGCCAAAGGG TTTTCAGCTC ATATCGGCAC 
GCGCCCCAAC TCGCTGAAGC CCTTATTACA GTGTTCGTGG ATATAGAGTT CACCGGTGAT 
CCACATCAGT TTGAGCAGAA GTTTAATTAC AGGAGGCCAA TGTACGCCAT TCTGAAGTAC 
ATGTGGGGAG AGAAGAGTTA CAGAGAGAGC ATTAAGAATC TTGCCGACTA TGCCTCTGAA 
AACCTTGAAG CCATGAATCC ACCACTCTTC CTTAGGTTTC TCAATCTCCT CATGAATGAT 
GCCATCTTCT TACTTGACGA GGCTATACAG TACTTAAGTA AGATCAAAAT CCTCCAGCTG 
GAGCGGGATC GGGGGGAGTG GGACAGTCTG GCCCCTGACG CCCGCAGAGA GAAAGAATCG 
AGTCTGCAGA TGTTTGGACA GCTGGGTCGC TTCCACAACA TCATGTCAAA TGAGACCATT 
GGCACCTTGG CTTTCCTCAC ATCTGAGATA AAAGGTCTTT TTGTTCACCC CTTTCTTGCT 
GAGAGGATCA TCTCCATGCT CAACTACTTT TTACAGCACC TGGTAGGCCC CAAAATGGGC 
GCCTTGAAGG TTAAGGACTT CAGTGAATTT GACTTCAAAC CCCAACAGCT TGTGTCGGAC 
ATCTGTACTA TTTACCTAAA CTTGGGAGAT GAAGAAAATT TCTGCGCCAC CGTCCCAAAA 
GATGGAAGGT CATATTCACC CACTCTGTTC TGTCAAACTG TCCGCGTCCT CAAGAAAATC 
AACAAGCCTG GTGACATGAT TATATCTTTC AGTCTCCTAG CAGATAAAAT TAAGTCTCTT 
GCAGACAGGC ATCAGCAAGA GGAAGAGACG TTCTCTGATG CTCCAGATGA ATTTCTGGAT 
CCCATTATGT CTACCTTGAT GCTCGATCCT GTGCTTTTAC CGTCTTCAAA CGTCACAGTG 
GACCGCTCAA CAATAGCACG ACACTTACTA AGTGACCAGA CAGACCCATT CAACCGCAGT 
CCGCTAACCA TGGACCAGAT CAGGCCCAAC GAAGAGCTCA GGCAACAGAT CATGAAGTGG 
CTTGCTGATC ATAAGCAAAG CAACCAGCAA ATGGGACCCA GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200095.2
CDS	117..3344
Translation	MTDQGNNNQNISQNPFAALFSSVADAKQFASDQKQQRPTEQQLDSGESQSESENSASESIDENDDSVAEISRSFRSRQELCEQLNVNHMIQRIFLITLDNSDPSLRSGNGIPPRCVYLEEMAADLDGQDWLNMDTIEQALFNRLLLQEPGNHLIYMTSCSVVNLSADRDAGEKRAIPYLYACYQRAKEEITKVPEKLLSFAVHCKNLTVSNARTVLLTPEIYISQNVYEQLLDLLLEAVRGAQFEEVVEFLEEVIASLLADQEVRTFGEVMVPVFDIFHGRVKDLDLCQLLLFSYLEILLYFSRQKDIAKVLMEHIQPKDPNNGIQYQKTLLGAILNISCLLKTPGVVENHGFFLNPSRSSPQELKVQESNIHQFMGQFHDKLYQILKNLLQQSGETRHLLLSWLGGCLQANMGRAKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFSRPYSPKLLTFNPTYCLLKELSEEERRNRNVHARGLDKETCLIPVPPQQTVEFAQSYSLLTENLILTQLTLYLGFHRLHDQMVKMNQSLHRLQGTWRDTQLSGGPAAAELREQFEHLMTVYLSTKAATTQPTMLQNCLNLQASCAALLVQLSLGNQGPEHIPLTFPLPSLENSLLCYVPEFFAENMGDFFIFLRRFADEVLESSAESLEHVLTFITVLMGNVDRMKNPHLRAKLAEVLEAVMPHMETLSPGAAQPIMFQRQRVFSSYRHAPQLAEALITVFVDIEFTGDPHQFEQKFNYRRPMYAILKYMWGEKSYRESIKNLADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKILQLERDRGEWDSLAPDARREKESSLQMFGQLGRFHNIMSNETIGTLAFLTSEIKGLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFCQTVRVLKKINKPGDMIISFSLLADKIKSLADRHQQEEETFSDAPDEFLDPIMSTLMLDPVLLPSSNVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNEELRQQIMKWLADHKQSNQQMGPSG

Target ORF information:

RefSeq Version	XM_003200047.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ubiquitination factor E4A (UFD2 homolog, yeast) (ube4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200047.4

ORF Insert Sequence:

ATGACAGACC AGGGCAATAA CAATCAGAAC ATCTCACAAA ACCCTTTCGC AGCACTCTTC 
AGTTCAGTTG CTGACGCAAA ACAGTTTGCA TCTGACCAAA AGCAACAGCG TCCAACTGAG 
CAGCAATTAG ATTCAGGAGA GAGCCAGTCT GAGTCAGAAA ACTCTGCGTC TGAAAGCATT 
GATGAGAATG ATGACTCAGT GGCGGAGATC AGCCGATCGT TCCGCTCACG ACAGGAGCTC 
TGTGAACAGC TCAATGTCAA CCACATGATC CAGAGGATCT TCCTCATCAC TCTGGACAAC 
AGTGATCCGA GTCTAAGAAG TGGCAATGGC ATCCCACCAC GCTGTGTTTA CCTTGAGGAA 
ATGGCAGCTG ATCTTGATGG TCAGGACTGG CTTAACATGG ACACCATAGA GCAGGCTTTG 
TTCAACAGGC TGCTTTTGCA GGAGCCAGGA AACCATCTCA TTTACATGAC CTCCTGTAGT 
GTAGTGAATC TCTCGGCAGA TCGAGACGCT GGAGAGAAAC GTGCCATACC TTACCTTTAC 
GCCTGCTACC AAAGAGCTAA AGAGGAGATC ACTAAAGTCC CTGAGAAACT GTTGTCATTT 
GCTGTGCACT GCAAAAACCT GACCGTGTCC AATGCACGAA CTGTGCTGCT GACCCCAGAG 
ATCTACATCA GTCAGAACGT TTACGAGCAG CTTCTGGACC TGTTGCTGGA GGCTGTCAGA 
GGAGCCCAGT TTGAAGAGGT TGTGGAGTTT TTAGAGGAGG TCATTGCTAG CCTGTTGGCT 
GACCAAGAAG TGCGGACCTT TGGGGAGGTA ATGGTGCCAG TGTTTGATAT TTTCCATGGC 
CGGGTTAAAG ACCTAGACCT CTGTCAGCTG CTGCTGTTTT CCTACCTGGA AATTCTCCTG 
TACTTCAGTC GACAAAAGGA CATTGCTAAG GTTTTAATGG AGCACATTCA GCCCAAAGAC 
CCTAACAATG GGATTCAGTA TCAGAAAACA CTTCTGGGAG CCATCCTTAA TATTTCATGT 
CTGCTGAAAA CTCCTGGTGT GGTTGAAAAC CATGGCTTTT TCCTGAATCC ATCTCGCTCC 
AGCCCACAGG AATTGAAGGT TCAAGAGTCA AATATACACC AGTTTATGGG GCAGTTTCAT 
GATAAGCTCT ATCAGATCCT GAAAAACTTG CTCCAGCAGT CCGGCGAGAC TCGCCACCTG 
CTGCTCTCGT GGCTGGGTGG CTGCCTGCAG GCCAACATGG GTCGGGCCAA AATCTGGGCA 
AACCAGATGC CCGAGATATT CTTCCAAATG TATGCATCGG ATGCATTCTT CTTAAATCTG 
GGAGCCGCTC TGCTCAAGCT CTGCCAGCCG TTTTCTCGAC CCTACTCTCC TAAACTCCTG 
ACCTTTAACC CCACATACTG CTTGCTTAAG GAGCTGAGCG AAGAAGAGCG GCGTAACAGA 
AACGTGCATG CTAGAGGTCT GGACAAGGAG ACATGTCTGA TACCTGTGCC CCCTCAACAA 
ACGGTTGAAT TTGCCCAATC ATACAGCCTT CTGACAGAAA ACCTCATTCT TACACAACTC 
ACCCTGTACC TTGGCTTCCA TAGACTTCAT GATCAGATGG TGAAGATGAA TCAGTCTCTG 
CACCGGCTCC AGGGGACGTG GCGGGACACG CAGCTCAGTG GAGGCCCAGC AGCAGCAGAG 
CTGCGGGAAC AGTTTGAACA CCTTATGACC GTCTATCTCT CAACCAAGGC TGCAACCACA 
CAGCCCACCA TGTTGCAGAA CTGCCTCAAC CTCCAGGCCT CCTGCGCCGC CCTGCTGGTT 
CAGCTCAGCC TGGGCAATCA GGGCCCTGAA CACATTCCTC TCACCTTCCC TTTACCTTCA 
CTAGAGAACA GCCTGCTTTG CTATGTGCCA GAGTTCTTTG CTGAAAACAT GGGTGATTTC 
TTCATATTCC TGCGCCGCTT TGCTGATGAG GTGTTGGAGT CCTCTGCGGA AAGCTTAGAA 
CACGTCCTGA CCTTCATCAC TGTGCTCATG GGCAATGTTG ATAGAATGAA GAATCCTCAT 
TTGCGGGCAA AGTTGGCAGA GGTCCTTGAG GCTGTGATGC CCCATATGGA GACTCTGTCA 
CCTGGCGCTG CCCAACCTAT CATGTTTCAG CGCCAAAGGG TTTTCAGCTC ATATCGGCAC 
GCGCCCCAAC TCGCTGAAGC CCTTATTACA GTGTTCGTGG ATATAGAGTT CACCGGTGAT 
CCACATCAGT TTGAGCAGAA GTTTAATTAC AGGAGGCCAA TGTACGCCAT TCTGAAGTAC 
ATGTGGGGAG AGAAGAGTTA CAGAGAGAGC ATTAAGAATC TTGCCGACTA TGCCTCTGAA 
AACCTTGAAG CCATGAATCC ACCACTCTTC CTTAGGTTTC TCAATCTCCT CATGAATGAT 
GCCATCTTCT TACTTGACGA GGCTATACAG TACTTAAGTA AGATCAAAAT CCTCCAGCTG 
GAGCGGGATC GGGGGGAGTG GGACAGTCTG GCCCCTGACG CCCGCAGAGA GAAAGAATCG 
AGTCTGCAGA TGTTTGGACA GCTGGGTCGC TTCCACAACA TCATGTCAAA TGAGACCATT 
GGCACCTTGG CTTTCCTCAC ATCTGAGATA AAAGGTCTTT TTGTTCACCC CTTTCTTGCT 
GAGAGGATCA TCTCCATGCT CAACTACTTT TTACAGCACC TGGTAGGCCC CAAAATGGGC 
GCCTTGAAGG TTAAGGACTT CAGTGAATTT GACTTCAAAC CCCAACAGCT TGTGTCGGAC 
ATCTGTACTA TTTACCTAAA CTTGGGAGAT GAAGAAAATT TCTGCGCCAC CGTCCCAAAA 
GATGGAAGGT CATATTCACC CACTCTGTTC TGTCAAACTG TCCGCGTCCT CAAGAAAATC 
AACAAGCCTG GTGACATGAT TATATCTTTC AGTCTCCTAG CAGATAAAAT TAAGTCTCTT 
GCAGACAGGC ATCAGCAAGA GGAAGAGACG TTCTCTGATG CTCCAGATGA ATTTCTGGAT 
CCCATTATGT CTACCTTGAT GCTCGATCCT GTGCTTTTAC CGTCTTCAAA CGTCACAGTG 
GACCGCTCAA CAATAGCACG ACACTTACTA AGTGACCAGA CAGACCCATT CAACCGCAGT 
CCGCTAACCA TGGACCAGAT CAGGCCCAAC GAAGAGCTCA GGCAACAGAT CATGAAGTGG 
CTTGCTGATC ATAAGCAAAG CAACCAGCAA ATGGGACCCA GTGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ube4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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