raf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	raf1b
Entrez Gene ID	557109
Full Name	Raf-1 proto-oncogene, serine/threonine kinase b
General protein information	Preferred Names Raf-1 proto-oncogene, serine/threonine kinase b Names RAF proto-oncogene serine/threonine-protein kinase v-raf-1 murine leukemia viral oncogene homolog 1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021479627.1	XP_021335302.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_017358232.1	XP_017213721.1	RAF proto-oncogene serine/threonine-protein kinase isoform X2
XM_021479626.1	XP_021335301.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_017358231.2	XP_017213720.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_017358231.1	XP_017213720.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_021479625.1	XP_021335300.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
NM_001199755.1	NP_001186684.1	RAF proto-oncogene serine/threonine-protein kinase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	raf1	NP_001017117.1
Homo sapiens (human)	RAF1	NP_002871.1
Canis lupus familiaris (dog)	RAF1	XP_005632198.1
Mus musculus (house mouse)	Raf1	NP_084056.1
	RAF1	XP_001086154.1
Rattus norvegicus (Norway rat)	Raf1	NP_036771.1
Gallus gallus (chicken)	RAF1	NP_990638.1
Pan troglodytes (chimpanzee)	RAF1	XP_516289.2
Danio rerio (zebrafish)	raf1b	NP_001186684.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Differential expression of synapsin genes during early zebrafish development.
Garbarino G, Costa S, Pestarino M, Candiani S
Neuroscience280351-67(2014 Nov)

Identification of multipath genes differentially expressed in pathway-targeted microarrays in zebrafish infected and surviving spring viremia carp virus (SVCV) suggest preventive drug candidates.
Encinas P, Garcia-Valtanen P, Chinchilla B, Gomez-Casado E, Estepa A, Coll J
PloS one8(9)e73553(2013)

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
Sato Y, Hashiguchi Y, Nishida M
BMC evolutionary biology9127(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following raf1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the raf1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38980	NM_001199755.1 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa16596	XM_021479627.1 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa16596	XM_017358231.2 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa16596	XM_021479625.1 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa16596	XM_021479626.1 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa54269	XM_017358232.1 Latest version!	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa16596	XM_017358231.1	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

raf1b ( NM_001199755.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186684.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), mRNA.

Price & Availability↑

CloneID	ODa38980
Clone ID Related Accession (Same CDS sequence)	NM_001199755.1
Accession Version	NM_001199755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR855375.8, BC097130.1 and CU695207.5. On Dec 17, 2010 this sequence version replaced XR_029877.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01032165.1, GDQH01001700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCT 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186684.1
CDS	97..1986
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	NM_001199755.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199755.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCT 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_021479627.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335302.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa16596
Clone ID Related Accession (Same CDS sequence)	XM_021479627.1 , XM_021479626.1 , XM_017358231.2 , XM_017358231.1 , XM_021479625.1
Accession Version	XM_021479627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335302.1
CDS	389..2278
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	XM_021479627.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479627.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_021479626.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335301.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa16596
Clone ID Related Accession (Same CDS sequence)	XM_021479627.1 , XM_021479626.1 , XM_017358231.2 , XM_017358231.1 , XM_021479625.1
Accession Version	XM_021479626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335301.1
CDS	366..2255
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	XM_021479626.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479626.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_017358231.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213720.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa16596
Clone ID Related Accession (Same CDS sequence)	XM_021479627.1 , XM_021479626.1 , XM_017358231.2 , XM_017358231.1 , XM_021479625.1
Accession Version	XM_017358231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358231.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213720.1
CDS	271..2160
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	XM_017358231.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358231.2

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_017358231.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213720.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16596
Clone ID Related Accession (Same CDS sequence)	XM_021479627.1 , XM_021479626.1 , XM_017358231.2 , XM_017358231.1 , XM_021479625.1
Accession Version	XM_017358231.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213720.1
CDS	272..2161
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	XM_017358231.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358231.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_021479625.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335300.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16596
Clone ID Related Accession (Same CDS sequence)	XM_021479627.1 , XM_021479626.1 , XM_017358231.2 , XM_017358231.1 , XM_021479625.1
Accession Version	XM_021479625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335300.1
CDS	389..2278
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLRKTRHVNIVLFMGYMTKVKLAIVTQWCEGSSLYKHLHVQETNFQMFQLMDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKARWSGSHRVEQPSGSILWMAPEVIRMQDNNPYSFQSDVYSYGIVLYELMTGELPYSMIGNRDQIIFMVGRGYLSPDLSKLYKSCPKAMKRLVSDCIQKTKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRATHTEDMNVCTLALTSAFK

Target ORF information:

RefSeq Version	XM_021479625.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479625.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGAAGACCA GGCATGTTAA TATCGTCTTG TTTATGGGCT ACATGACGAA GGTGAAGCTG 
GCCATCGTGA CGCAGTGGTG TGAGGGAAGC AGCCTCTACA AACACCTGCA TGTGCAGGAG 
ACAAACTTCC AGATGTTCCA GCTGATGGAC ATCGCCAGAC AGACGGCTCA GGGCATGGAT 
TACCTGCATG CAAAGAACAT CATCCACCGA GACATGAAGT CCAACAATAT CTTCCTGCAC 
GAGGGTCTGA CGGTGAAGAT TGGAGACTTC GGTTTGGCCA CAGTAAAGGC TCGCTGGAGC 
GGCTCTCATA GAGTCGAGCA GCCGTCTGGA TCCATTCTGT GGATGGCTCC TGAAGTGATC 
CGCATGCAGG ATAATAACCC GTACAGCTTC CAGTCAGACG TGTACTCGTA CGGGATCGTC 
CTGTATGAGC TGATGACCGG AGAACTGCCT TATTCTATGA TTGGAAACAG AGATCAGATC 
ATCTTTATGG TGGGTCGGGG TTATCTGTCT CCAGACCTCA GTAAGCTGTA TAAGAGCTGT 
CCTAAAGCCA TGAAGAGACT GGTGTCCGAC TGCATCCAGA AGACCAAAGA GGAGCGCCCA 
CTCTTCCCAC AGATCTTGTC CTCTATCGAG CTCCTCCAGC ATTCGCTCCC CAAGATAAAC 
CGCAGCGCGT CTGAACCGTC GCTGCACAGA GCCACACACA CCGAGGACAT GAACGTCTGC 
ACGCTGGCAC TCACCTCTGC CTTTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

raf1b ( XM_017358232.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213721.1 Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54269
Clone ID Related Accession (Same CDS sequence)	XM_017358232.1
Accession Version	XM_017358232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213721.1
CDS	728..1873
Translation	MEHFQGAWKSVSSHGFGCKDSVFEDSCMSPSILKTFPYRRLSDDGRIPDSKTSSTIRVFLPNKQRTVVNVRAGMTLRNCLIKALKVRGLQPECCAVFKLQREHSAKKLRMDWSTDSTSLVGEELLVEVLDHVPLTTHNFVRKTFLKLAFCDICQKFLLHGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPTPGENGALTLPPLSSRRMQNSRFSFSPYHRYSTPNALNYITPLPSPGVSLGHKVLSTSTPNVHMVSTTQPVDEAAYQEALRHDESGSPQNLSPTEWSKTPAPAHREKAPSSNTQEKRIRPREKRDSSYYWEIEATEVLLLSRIGSGSFGTVYKGKWHGDVAVKILKVINPTPEQLQAFRNEVAVLR

Target ORF information:

RefSeq Version	XM_017358232.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Raf-1 proto-oncogene, serine/threonine kinase b (raf1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358232.1

ORF Insert Sequence:

ATGGAGCATT TTCAGGGGGC CTGGAAGAGC GTCAGCAGTC ACGGCTTCGG GTGTAAAGAC 
TCAGTATTCG AGGACTCCTG CATGTCTCCC AGCATCCTCA AGACCTTCCC GTACCGTAGA 
TTATCCGACG ATGGCAGAAT CCCAGACTCC AAGACCAGCA GCACCATCCG CGTCTTCCTG 
CCCAACAAGC AGCGCACCGT GGTGAACGTG CGAGCAGGAA TGACGCTGCG CAACTGTCTC 
ATCAAAGCCC TGAAAGTTCG AGGTCTTCAG CCTGAATGCT GTGCTGTTTT CAAGCTTCAG 
CGGGAACATT CTGCTAAGAA GCTGCGCATG GACTGGAGCA CTGATTCTAC CTCACTGGTC 
GGAGAGGAGC TGCTGGTTGA AGTCTTAGAT CACGTGCCAT TGACCACGCA CAATTTTGTA 
CGCAAAACAT TTTTGAAGTT GGCCTTTTGT GACATCTGTC AGAAGTTTCT TTTGCACGGC 
TTCAGATGTC AGACGTGCGG ATACAAGTTT CATGAGCACT GCAGCACCAA AGTGCCAACC 
ATGTGTGTGG ACTGGAGCAA CATTCGCCAA CTCCTGCTGT TCCCGACTCC TGGAGAGAAC 
GGGGCTCTGA CCCTCCCTCC GCTCTCGTCC CGTCGCATGC AAAACTCTAG ATTCTCCTTC 
AGTCCCTATC ACAGATACTC AACGCCAAAT GCATTGAACT ACATCACACC CCTGCCGTCC 
CCTGGAGTCT CTCTGGGCCA TAAAGTCTTG TCCACATCCA CACCCAATGT CCACATGGTG 
AGCACAACCC AGCCAGTCGA TGAAGCTGCT TATCAGGAAG CCTTAAGGCA TGATGAATCA 
GGAAGCCCTC AAAACTTAAG CCCGACCGAA TGGTCCAAAA CCCCAGCTCC GGCTCACAGG 
GAGAAAGCGC CTTCGAGTAA CACTCAGGAA AAACGAATCC GTCCTCGAGA GAAGAGAGAC 
TCCAGCTACT ACTGGGAGAT CGAGGCCACT GAGGTGCTTC TGCTCTCCCG GATCGGCTCA 
GGCTCTTTCG GCACAGTTTA CAAAGGGAAA TGGCACGGTG ATGTTGCTGT GAAGATTTTA 
AAGGTCATCA ATCCAACACC GGAGCAGTTG CAGGCCTTTC GCAATGAAGT GGCTGTTCTC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Differential expression of synapsin genes during early zebrafish development.
Neuroscience280351-67(2014 Nov)
Garbarino G,Costa S,Pestarino M,Candiani S

Identification of multipath genes differentially expressed in pathway-targeted microarrays in zebrafish infected and surviving spring viremia carp virus (SVCV) suggest preventive drug candidates.
PloS one8(9)e73553(2013)
Encinas P,Garcia-Valtanen P,Chinchilla B,Gomez-Casado E,Estepa A,Coll J

Temporal pattern of loss/persistence of duplicate genes involved in signal transduction and metabolic pathways after teleost-specific genome duplication.
BMC evolutionary biology9127(2009 Jun)
Sato Y,Hashiguchi Y,Nishida M

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raf1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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