igf2r cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	igf2r
Entrez Gene ID	557061
Full Name	insulin-like growth factor 2 receptor
Synonyms	si:ch211-214g8.2
General protein information	Preferred Names insulin-like growth factor 2 receptor Names cation-independent mannose-6-phosphate receptor CI-MPR cation-independent mannose 6-phosphate receptor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039627.2	NP_001034716.1	cation-independent mannose-6-phosphate receptor precursor
XM_021468430.1	XP_021324105.1	cation-independent mannose-6-phosphate receptor isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IGF2R	NP_776777.1
Xenopus tropicalis (tropical clawed frog)	igf2r	XP_002933917.2
Mus musculus (house mouse)	Igf2r	NP_034645.2
Danio rerio (zebrafish)	igf2r	NP_001034716.1
	IGF2R	XP_002804004.1
Canis lupus familiaris (dog)	IGF2R	NP_001116074.1
Pan troglodytes (chimpanzee)	IGF2R	XP_003311618.2
Homo sapiens (human)	IGF2R	NP_000867.2
Rattus norvegicus (Norway rat)	Igf2r	NP_036888.1
Gallus gallus (chicken)	IGF2R	NP_990301.1

Gene Ontology
Bibliography

Related articles in PubMed

Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish.
Zhu Y, Su G, Yang D, Zhang Y, Yu L, Li Y, Giesy JP, Letcher RJ, Liu C
Environmental pollution (Barking, Essex : 1987)229470-478(2017 Oct)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Effects of microgravity simulation on zebrafish transcriptomes and bone physiology-exposure starting at 5 days post fertilization.
Aceto J, Nourizadeh-Lillabadi R, Bradamante S, Maier JA, Alestrom P, van Loon JJ, Muller M
NPJ microgravity216010(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the D. rerio CI-MPR harbors three glycan-binding sites that map to domains 3, 5 and 9 of its 15-domain-containing extracytoplasmic region
Title: The glycan-binding properties of the cation-independent mannose 6-phosphate receptor are evolutionary conserved in vertebrates.

The expression patterns suggest time- and tissue-specific functions for the M6P receptors, with particular evidence for roles in neural development.
Title: Mannose 6-phosphate receptors in an ancient vertebrate, zebrafish.

The following igf2r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the igf2r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa25174	XM_021468430.1 Latest version!	Danio rerio insulin-like growth factor 2 receptor (igf2r), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa35507	NM_001039627.2 Latest version!	Danio rerio insulin-like growth factor 2 receptor (igf2r), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

igf2r ( XM_021468430.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324105.1 Danio rerio insulin-like growth factor 2 receptor (igf2r), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25174
Clone ID Related Accession (Same CDS sequence)	XM_021468430.1
Accession Version	XM_021468430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cation-independent mannose-6-phosphate receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCGTG TTGGATTTTG CGGAGTCGCT GCTACTGTTA TTATTAATAC TTTTCTATTT 
CTACAATGCG TGTCGTCAGC GGCGGCTTCG GATGACAGCC TATGGTACCA GGATCTGTGC 
AGTTACAAAT GGGAGGCCAT TGATAAGGAC AACAATGTGA GCTACGCTCT GAAGCTTTGC 
GATTCATCTC CAAAAACAGA ATGTGGAGAA GGAAGTGCGG TCTGTGCCCA CTATCTTACA 
AGTGGGCAGC ATCAGTCAGT GGGTGAGCTC TCTCTGACGA AGGTCTCGCC TGCTGTGCTC 
GATTTCAACA GCTCGAAGAA ATGCGAAGAC AAGAAGAGCA ATGTTCAGAG CAGCATCACC 
TTTCAATGCG GCAAAACAAT GGGCACGCCT GAGTTTGTCA CCGTCTCCGA GTGTGTGCAT 
TACTTTGAGT GGAGGACGTA TGTCGCCTGC AGACGTGATA AATTTAAACC GCATAAAGAG 
GTCCCCTGCT ATGTCTTTGA CTCAGATGGA AAGAAGCATG ACCTCAATCC GCTGATAAAA 
CTCTCAGATG GCTACTTAGT TGACGACCCG GATGACGAGA TAGATTTCTA CATTAATATA 
TGCAGAAGTC TAAATCGTGC TGGAAGCACC TGTACTGAAG GATCTGCCGC CTGTCTCACT 
CACACCTCTG GTAAAGGCTC ATTTGACATG GGTCAACCTG TTCACCAGCT CGAACTGCTC 
TCTAATGACA AACTAAGACT TCGATACGAG GGAGTCAAAG AAAGCCCAGA CTTCTGTGAT 
GGCCACAGTC CAGCAGTGAC CATTACATTT ATTTGTCCCT CCACAAGGCA GACGGGAAGT 
AACCCTAAGA TGATCAGCAG CACGAACTGT CGATATGAGA TTGAGTGGGT GACGGAGTAC 
GCCTGCCATA GAGATTACCT GGAGAGTCAC GACTGCAAAC TGACCAGCAC TCAGCATGAC 
ATCTCCATCG ACCTCACGCA CCTGACGCAC GGCTCCACAG ATACACCATA CATGGCTGAG 
GCCAAAAATG GCAAGGACAC CTACTTTTTC TATCTGAACG TGTGTGGAGG GACCAATGCT 
GGACAGTGTT TTGACGACAA AGGATACATC TCTGCCTGCC AATTCAAAGA GAATGGCGAT 
GTAAAGAAGA TTGCTGGAAG ATACCAGAAC CAAACCCTGA GATATTCAGA TGGTGATCTC 
ACATTAATCT ACCCAGACGG CAGCAGATGC AGCACTGGAT TCCAGCGCAT GACTATTATT 
AACTTTGAGT GCAATGCAAC TGCTGGGAAC GGTCAGCCGG TTTTTACAGG AGAGTCTGAC 
TGCACGTATT ACTTTGACTG GCAGACGTCG TACGCATGTG TGGAGAAGGA AGACCTGCTG 
TGTCGAGTCA CAGACCACAA GAAGAGATAT GATTTATCTC CTCTTACACG ATTCCCAGAA 
TCGGAGGGAT CTCAGAATTG GGTGGCGGTG AACGCAAAAG CAGCAGAGTC TGATAAAAAG 
CGCATTTATA TGAATGTCTG TCATAAAATC ATCCAGGATG GGGTGGCCAG TTCTTGCCCT 
GAGGACTCTG CAATATGTGC CATAGGAAAT GGGACAGCAA AGAATCTGGG CAAATTCCTG 
AAATCTCCTG AAAAGGCAAC CGAGGGTGAC GACATCAGAC TGATTTACAC AGACGGAGAC 
AAGTGTAGAG AGAAGATGAA GGTGAAGAGC ATCATCACTC TGAAGTGTAA GCCTGGAGAT 
GTGGAAAGTC CTCCAGTCCT CAGGAGTGTG TCCAGCGACG GCTGCATCTA TGAGTTTGAG 
TGGTTTACCG CTTCAGCCTG TGTCCTGTCC AAAACTGAAG GAGACAACTG CAAGGTGGAG 
GACATGCAAA CAGGAATTTC GTTTGATTTG TCTCCGCTTC AGAAATCTAA TGGAGGCTTT 
TATAACATGT CCAGTAAGAA GTATGACTAC TTCATAAACG TATGTGGAAA TGTTAAAGCC 
GCACAGTGTC CAGAAACAGC AGGGGCTTGT CAAGTTGACC AGACCGGCAA ATCGTCTTGG 
AATCTCGGCC AGTTCAACTC CAAGCTTTCT TATTATGATG GCATGATTCA GCTGACCTAC 
AGAAACGGCT CTCAGTACAA CAACAAGCAG CACACGCAGC GATCCACACA CATCTCCTTC 
CTCTGTGACC GAGAGGCTGG TCCAGGAAAA CCAGAGTTTC AGGATGAGGA TGAGTACACG 
TATAACTTTA AGTGGTACAC TTCCTACGCC TGTCCAGAGA GATTACATGA GTGTGTCGTG 
ACAGACCCCG AAACTCTTCA ACAGTATGAC CTGTCCAGTC TGTCAGTATC TAATGGCGGC 
AGGAACTGGG AGGTCATGGA CTCGTCTGAC ATCAGCAGCC TGAGGAAATA CTACATCAAT 
GTGTGTCGTC CTCTTAAAGC TGTGCCGGGC TGTGACAGAA GAGCTTCAGT CTGTGAGATG 
AAGTTCGAGG CTGACAGGGA AGGCCTGTCT GAGAAGGTGG AGGTCAGTAA TTTGGGCATT 
GCTAAGAAAG GGCCGGTGAT TGTGGAGCAA AACCAGCTGA TGTTGGAGTA CACCAAGGGC 
TCCATGTGTG AAGCAGATGG AAAGACCACC ACATACACAA CACGCATCCA CTTCGTCTGC 
GCTTCTGGGA CACCACCTTC TGGCCCTCGC TTTGTGGTGA ACCAGAATTG TACTGTTGAT 
TTTGTTTGGG ACACAGAAGC AGCCTGTGCC ATTTCTACTG TCGTGGGGAC AAACCAGACG 
TGCTCCGTGA AAGATCCCAA CACTGGCTTT GAATTCAACC TGCAGCCGCT GGCCACTGAC 
AAAGGATACC GCGTCAACGT TAATGGAAAA GACTTTATTG TGAACATTTG TGGTCCAGTG 
AAGGATTGTG AGACTGCTGG ATCTAAAAAT GTGTCCGGCT GTGAGCTCGA GGATCAGAAG 
CCGGTCAGTC AGGTTGGGGG GGAGCGTTCC CTCCAGTTCT CCACCGATGG GGCTCTCACT 
CTCACATACA AGGGGGTCAT GGACATACCC ACAGGTACCC AAGACACCTT CATTATTAAT 
TTTGTGTGTG ACCAAAAGGC CTCTCCACCC TCCCTGAAGC TTATACAGGA AGAGCTGGGC 
ACAAGTACAC ACGTAATCCA TAATGTGATT TTTGAGCTTG CAACTCCACT AGCCTGTAAA 
CCTGCACCTG TGGACTGCAC AGTCACTGAT TCACAGGGCC GGGAGTATGA CCTGGGTGAC 
CTCAGTCTGG ATGAGAAGTC ATATGTTCCT CTGGACACCT CGGATCAGGC CCGGTTTCAG 
AAGTTTTACG TAAATGTGTG TAAACCACTG CCTCGTGTTC AGGGCTGCCC TGCTGGTGCA 
ATTGGAGCCT GCGGACAAAT CAACAGCAGT TTTGTAAATT TGGGATACGT GCAGTCAAAC 
CTCCAGGCCG CAGCAGACGG CTCCATCAGT ATTGTTTATC TGAACGGGGA CAAGTGTGGG 
ACATCAGGAC GTTACTCCAC CAGAATCATC TTCCAGTGTG ATGACAGTCC TGGTGCACCG 
ATGTTCGATC GCAAAGATGG CTGTGAGTTT GTTTTCATCT GGAGAACCTC AGAAGCTTGC 
CCAATCAAAA GAGTCCATGG TGAAAACTGC AAAGTCACAG ACCCCAAGAG CGGTTATGAG 
TACAATCTCA CACCGTTGGC TGGACAGGAC TATGAAGTGA AGAGTTCGAC CTATGAGTAT 
CATTTCGCAG TCTGTGGCCC AATTACAACC TCTGTCTGCC TTCACGATGC CAGCCAATCT 
GTGTCCTCCT GTCAGGTCGA GAACCAAAAG CACAGGATTG CAGGAATAGC CAATCAAAAC 
CTCACGTTTG ACGATGGGAT CATTATGATC AACTATACAA ACGGGGAAAC ATGTCACAAG 
ATTTACGAGC GGTCCACAGC TATTCTATTC TCCTGTGACC ACAGCCGGAA TCCTGGTAAG 
CCAGATTTCA TAAAGGAGAC CGCAGACTGC ACATATCTGT TTGAGTGGCA CACAGCTTTG 
GCCTGCCCTT CCTTTAAAAC CACAACCTGC TCCTACAATG ACGGCAGTGG CCACTCCTAC 
GATCTCTCCT CATTGGCCCT GCACAAATCT AATTGGATTG TTGTGCCAGA ATCATCGAAT 
CAGAAGCAGC GTTACTATAT CAATGTGTGC AAGTCTCTGG TCCCTCAGAC AGGTTTGTGG 
AGCTGCCCGT CCAGTGCTGC AGCCTGTCTG AAGGATGGGG ACGAGTATGT GAACCTGGGG 
GAGGCGGAGT CTGGTCCACA GTGGGATAAA AACGTCCTGG TTCTGAAGTA TACCAACGGA 
AAAGCCTGTC CTGATGGCAA GAGAAACCGG ACCACCATTA TTCGCTTCAA ATGCAATCCA 
GACAAAGTGG ATTCTGAACC CACTCTGATC ACAGCTCTGG AAAACTGTGT CTACAGCTTC 
GTATGGTTCA CAGCCGCAGC CTGTCCTCTC AACAGCACTG AGCATGGAGA CTGTAAAGTC 
ACAAATCCTG CTACAGGTCA TCTGTTTGAT CTTAATGCGC TCAGTCGGGC GGGTGGATAT 
ACGGTTTATG ATCCTGAATC ACACAGAAAG ATGTTCAGAC TGAATGTGTG TGGTGAAATA 
ATCAATGCTG GTTGTGCTAC TGGAACAGGC GTGTGCATCA AGGACAATCA GATGGCCATA 
AGTGCTGGAA AAGCCTCAAG AAAGCTGGTT TATAAGAATC AGGTGGTGGA GCTCAGTTAT 
GAGGACGGGG ATGCATGCTC TACAAATTCC CGCAAACACA AGAGCATCTT TAGCTTCGTC 
TGCAAGTCTG AAGGAGGAGG AACTGATGGA CCCGTGCTGG TTTACTCAGA CGACACAACC 
TGCACTCACT TCTTCACCTG GCACACTCCT CTAGTCTGCG AACAACAGGT GAAGTGCTCT 
GTTTGGAACG GTACCAACCA GATTGACCTC AGTCCTCTAA TACACCTAAC CGGTTACTAC 
ACTGCTATAG ATGAGGATGT GGACCGGGAC AAATCACCTG ACTTCTACAT TAACATCTGC 
CAGCCCCTCA ACCCCATCCC AAGGGTCAAC TGTCCTCCAG GGGCCGCTGT GTGCATGGAC 
CCTGTGGATG GAGAACCAAT TGACATTGGA CGAATCACAT CTCCTCCTCG CTATAACAGT 
GAATCTAAAG AGGTGGAGAT CACCTTCAGC AGCACAACTG TCTGCGCCTC TAATCGGACC 
TCCAACTACT CCTCCAAGAT CATTTTCACC TGCCAGAAAG GAGCAGAGCT GGGTTCTCCT 
CGGATGATTC GGGCTCACAG CTGCATGTAT GTGTTTGAAT GGGCCACTCC TGTGGTTTGT 
CCGGAGATCA TCGAGGCGCA GGGCTGCAAT CTGACCGTCT CTCAGCTGCG CTACACCTTT 
GACCTCTCAG CACTGACTGG ACCTGTTCAG GTTCCTGTAA CTTCTGGCTC TTTCAGCATC 
AACGTGTGTG GGACTGTGGC GAACACGACT TGTAAAGACA GTGCAGTGTG TCTGATTTCC 
AGTGGCTCAA GAGCTTCATA CGGGAACGCT AAACTCATGA ACCTGGACTA CAAGAGAGAA 
GAGCAGTCGG TGATAATGCA GTACAGCGGG GGAGACCCCT GTCCTCAAGG TGTGCAAGGG 
AAGCGTCAGT CTTCGATCTT GTTCACTTGT GATCGAGCGG CGGGTCGTGG AAGTCCACAG 
CTGATCAGCG ATACCCAGGG TTGCTCTGCC ACTTTTCAGT GGAGAACCAA TGTGGTTTGT 
CCACCCAGAA AGATGGAGTG CAAACTGGCA GGACAACACC AAACCTACGA CCTGCGCACA 
CTCTCTTCTC TAACAGAGCC CTGGAAGTTC AGCAATGGCC AAGACTCCTA TTATTTTAAT 
CTGTGTCAGG CCATTCACGG AGGTCAGCCG GGCTGTGCTG CGGAGGCTGC TGTGTGCCGT 
AGACTGGCCG GAGGAAAAAC AGAGACTCTG GGCCGGGTTC ACACGCAGAC CATGGAGTTA 
ATCGATGGAA AAATCCTGGT CAATTATTCC ATGGGCGACG AAGTGTGCGG AAACAAGAAA 
CCAGCCAAAA CAGTCATACA GCTGAAGTGT GGAAGTACAT TCGGCCATCC CAAACTACTG 
TTCAAGGAGG AAAAATCCTG TGAGTTCTGG TTTGAGTGGG AAACTCGATC AGCATGTGCT 
GTAAAGCAGG AGGAAGTGGA GATGGTCAAC GGGACCATTA AAGTTCCCAA AACAGGAGCC 
ACGTTCAGCT TGGGAGCCCT TTATTACCGT TTGCACAATG CCACAGGAGA CATCCGTGCC 
AATGGAGACC GCTATATCTA CCACATCCAG CTGTCGGGCA TCACGGACAG CTCCATTTCC 
ATGTGCTTGG GTGCCAATAT CTGCCAAGTC AAGATTAATG ACAGTTACCG CAGGAAAATC 
GGCTCCTCCA GCAAAGCCAA GTATTATATT AAAGGTGGAA ACCTTGACGT CTTGGTTCCG 
TCTGAATCCG TTTGTGGAAG AGACAAAACC AAAACTGTGT CTTCAGCAAT TCTGTTCCAC 
TGTAGCCCAA CGGCGGGAGA GGGCATCCCA GAATTCTTGC TGGAGACCGA TAGCTGCCAG 
TACCTGTTTG TGTGGCACAC CTCCACCGTT TGTGAATTTA TTTCTTCCAG CTCCATAGAC 
AGCACCGATG GAGGGGAGGA GCTTGCTGGA CTTTCTGGGC GGAGTCAGGC TTTAGGGGCG 
GTGCTTAGCC TGCTGTTGAT TGTGCTCACA ATCTGTTTGC TTGTTCTCCT GCTGCACAAA 
CAAGACAGGA GGCAACTTGT AATCCAGAAA GTGTCGAGCT GCTGCAGGAA AGGAAATCCA 
GTATCTTACA AATACTCCAG GGTGAACATG GATGAAGACG GGTGTGAAGA TGAGATGGAG 
TGGCTGATGG AGGAGACCGG GACCCCGACG CGAGAACCAC AGGAGAACGG TCACATCACC 
ACCAAACCGG TCCGTGCAGA CGCCCTGCGC TCCTTCTCCC TGGATGAACA GGACAGCGAA 
GATGAGGTGC TGACAGTCCC CGGGGTCCGA ATGGCCCCGT CATCCTCACG CCTAAAATCG 
GCATTACTCA AAGCAGAGAG CGACGAGGAT CTGGTAGGGC TCCTCGTGGA CACACGGAGC 
AAAGATAAGA GCCGATATGG GGATAAAAAC CAGACGAGAG CCCAGTTAGA TCCTGACGAC 
AGCGACGAGG ATCTGCTGAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324105.1
CDS	133..7359
Translation	MGRVGFCGVAATVIINTFLFLQCVSSAAASDDSLWYQDLCSYKWEAIDKDNNVSYALKLCDSSPKTECGEGSAVCAHYLTSGQHQSVGELSLTKVSPAVLDFNSSKKCEDKKSNVQSSITFQCGKTMGTPEFVTVSECVHYFEWRTYVACRRDKFKPHKEVPCYVFDSDGKKHDLNPLIKLSDGYLVDDPDDEIDFYINICRSLNRAGSTCTEGSAACLTHTSGKGSFDMGQPVHQLELLSNDKLRLRYEGVKESPDFCDGHSPAVTITFICPSTRQTGSNPKMISSTNCRYEIEWVTEYACHRDYLESHDCKLTSTQHDISIDLTHLTHGSTDTPYMAEAKNGKDTYFFYLNVCGGTNAGQCFDDKGYISACQFKENGDVKKIAGRYQNQTLRYSDGDLTLIYPDGSRCSTGFQRMTIINFECNATAGNGQPVFTGESDCTYYFDWQTSYACVEKEDLLCRVTDHKKRYDLSPLTRFPESEGSQNWVAVNAKAAESDKKRIYMNVCHKIIQDGVASSCPEDSAICAIGNGTAKNLGKFLKSPEKATEGDDIRLIYTDGDKCREKMKVKSIITLKCKPGDVESPPVLRSVSSDGCIYEFEWFTASACVLSKTEGDNCKVEDMQTGISFDLSPLQKSNGGFYNMSSKKYDYFINVCGNVKAAQCPETAGACQVDQTGKSSWNLGQFNSKLSYYDGMIQLTYRNGSQYNNKQHTQRSTHISFLCDREAGPGKPEFQDEDEYTYNFKWYTSYACPERLHECVVTDPETLQQYDLSSLSVSNGGRNWEVMDSSDISSLRKYYINVCRPLKAVPGCDRRASVCEMKFEADREGLSEKVEVSNLGIAKKGPVIVEQNQLMLEYTKGSMCEADGKTTTYTTRIHFVCASGTPPSGPRFVVNQNCTVDFVWDTEAACAISTVVGTNQTCSVKDPNTGFEFNLQPLATDKGYRVNVNGKDFIVNICGPVKDCETAGSKNVSGCELEDQKPVSQVGGERSLQFSTDGALTLTYKGVMDIPTGTQDTFIINFVCDQKASPPSLKLIQEELGTSTHVIHNVIFELATPLACKPAPVDCTVTDSQGREYDLGDLSLDEKSYVPLDTSDQARFQKFYVNVCKPLPRVQGCPAGAIGACGQINSSFVNLGYVQSNLQAAADGSISIVYLNGDKCGTSGRYSTRIIFQCDDSPGAPMFDRKDGCEFVFIWRTSEACPIKRVHGENCKVTDPKSGYEYNLTPLAGQDYEVKSSTYEYHFAVCGPITTSVCLHDASQSVSSCQVENQKHRIAGIANQNLTFDDGIIMINYTNGETCHKIYERSTAILFSCDHSRNPGKPDFIKETADCTYLFEWHTALACPSFKTTTCSYNDGSGHSYDLSSLALHKSNWIVVPESSNQKQRYYINVCKSLVPQTGLWSCPSSAAACLKDGDEYVNLGEAESGPQWDKNVLVLKYTNGKACPDGKRNRTTIIRFKCNPDKVDSEPTLITALENCVYSFVWFTAAACPLNSTEHGDCKVTNPATGHLFDLNALSRAGGYTVYDPESHRKMFRLNVCGEIINAGCATGTGVCIKDNQMAISAGKASRKLVYKNQVVELSYEDGDACSTNSRKHKSIFSFVCKSEGGGTDGPVLVYSDDTTCTHFFTWHTPLVCEQQVKCSVWNGTNQIDLSPLIHLTGYYTAIDEDVDRDKSPDFYINICQPLNPIPRVNCPPGAAVCMDPVDGEPIDIGRITSPPRYNSESKEVEITFSSTTVCASNRTSNYSSKIIFTCQKGAELGSPRMIRAHSCMYVFEWATPVVCPEIIEAQGCNLTVSQLRYTFDLSALTGPVQVPVTSGSFSINVCGTVANTTCKDSAVCLISSGSRASYGNAKLMNLDYKREEQSVIMQYSGGDPCPQGVQGKRQSSILFTCDRAAGRGSPQLISDTQGCSATFQWRTNVVCPPRKMECKLAGQHQTYDLRTLSSLTEPWKFSNGQDSYYFNLCQAIHGGQPGCAAEAAVCRRLAGGKTETLGRVHTQTMELIDGKILVNYSMGDEVCGNKKPAKTVIQLKCGSTFGHPKLLFKEEKSCEFWFEWETRSACAVKQEEVEMVNGTIKVPKTGATFSLGALYYRLHNATGDIRANGDRYIYHIQLSGITDSSISMCLGANICQVKINDSYRRKIGSSSKAKYYIKGGNLDVLVPSESVCGRDKTKTVSSAILFHCSPTAGEGIPEFLLETDSCQYLFVWHTSTVCEFISSSSIDSTDGGEELAGLSGRSQALGAVLSLLLIVLTICLLVLLLHKQDRRQLVIQKVSSCCRKGNPVSYKYSRVNMDEDGCEDEMEWLMEETGTPTREPQENGHITTKPVRADALRSFSLDEQDSEDEVLTVPGVRMAPSSSRLKSALLKAESDEDLVGLLVDTRSKDKSRYGDKNQTRAQLDPDDSDEDLLKV

Target ORF information:

RefSeq Version	XM_021468430.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin-like growth factor 2 receptor (igf2r), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468430.1

ORF Insert Sequence:

ATGGGTCGTG TTGGATTTTG CGGAGTCGCT GCTACTGTTA TTATTAATAC TTTTCTATTT 
CTACAATGCG TGTCGTCAGC GGCGGCTTCG GATGACAGCC TATGGTACCA GGATCTGTGC 
AGTTACAAAT GGGAGGCCAT TGATAAGGAC AACAATGTGA GCTACGCTCT GAAGCTTTGC 
GATTCATCTC CAAAAACAGA ATGTGGAGAA GGAAGTGCGG TCTGTGCCCA CTATCTTACA 
AGTGGGCAGC ATCAGTCAGT GGGTGAGCTC TCTCTGACGA AGGTCTCGCC TGCTGTGCTC 
GATTTCAACA GCTCGAAGAA ATGCGAAGAC AAGAAGAGCA ATGTTCAGAG CAGCATCACC 
TTTCAATGCG GCAAAACAAT GGGCACGCCT GAGTTTGTCA CCGTCTCCGA GTGTGTGCAT 
TACTTTGAGT GGAGGACGTA TGTCGCCTGC AGACGTGATA AATTTAAACC GCATAAAGAG 
GTCCCCTGCT ATGTCTTTGA CTCAGATGGA AAGAAGCATG ACCTCAATCC GCTGATAAAA 
CTCTCAGATG GCTACTTAGT TGACGACCCG GATGACGAGA TAGATTTCTA CATTAATATA 
TGCAGAAGTC TAAATCGTGC TGGAAGCACC TGTACTGAAG GATCTGCCGC CTGTCTCACT 
CACACCTCTG GTAAAGGCTC ATTTGACATG GGTCAACCTG TTCACCAGCT CGAACTGCTC 
TCTAATGACA AACTAAGACT TCGATACGAG GGAGTCAAAG AAAGCCCAGA CTTCTGTGAT 
GGCCACAGTC CAGCAGTGAC CATTACATTT ATTTGTCCCT CCACAAGGCA GACGGGAAGT 
AACCCTAAGA TGATCAGCAG CACGAACTGT CGATATGAGA TTGAGTGGGT GACGGAGTAC 
GCCTGCCATA GAGATTACCT GGAGAGTCAC GACTGCAAAC TGACCAGCAC TCAGCATGAC 
ATCTCCATCG ACCTCACGCA CCTGACGCAC GGCTCCACAG ATACACCATA CATGGCTGAG 
GCCAAAAATG GCAAGGACAC CTACTTTTTC TATCTGAACG TGTGTGGAGG GACCAATGCT 
GGACAGTGTT TTGACGACAA AGGATACATC TCTGCCTGCC AATTCAAAGA GAATGGCGAT 
GTAAAGAAGA TTGCTGGAAG ATACCAGAAC CAAACCCTGA GATATTCAGA TGGTGATCTC 
ACATTAATCT ACCCAGACGG CAGCAGATGC AGCACTGGAT TCCAGCGCAT GACTATTATT 
AACTTTGAGT GCAATGCAAC TGCTGGGAAC GGTCAGCCGG TTTTTACAGG AGAGTCTGAC 
TGCACGTATT ACTTTGACTG GCAGACGTCG TACGCATGTG TGGAGAAGGA AGACCTGCTG 
TGTCGAGTCA CAGACCACAA GAAGAGATAT GATTTATCTC CTCTTACACG ATTCCCAGAA 
TCGGAGGGAT CTCAGAATTG GGTGGCGGTG AACGCAAAAG CAGCAGAGTC TGATAAAAAG 
CGCATTTATA TGAATGTCTG TCATAAAATC ATCCAGGATG GGGTGGCCAG TTCTTGCCCT 
GAGGACTCTG CAATATGTGC CATAGGAAAT GGGACAGCAA AGAATCTGGG CAAATTCCTG 
AAATCTCCTG AAAAGGCAAC CGAGGGTGAC GACATCAGAC TGATTTACAC AGACGGAGAC 
AAGTGTAGAG AGAAGATGAA GGTGAAGAGC ATCATCACTC TGAAGTGTAA GCCTGGAGAT 
GTGGAAAGTC CTCCAGTCCT CAGGAGTGTG TCCAGCGACG GCTGCATCTA TGAGTTTGAG 
TGGTTTACCG CTTCAGCCTG TGTCCTGTCC AAAACTGAAG GAGACAACTG CAAGGTGGAG 
GACATGCAAA CAGGAATTTC GTTTGATTTG TCTCCGCTTC AGAAATCTAA TGGAGGCTTT 
TATAACATGT CCAGTAAGAA GTATGACTAC TTCATAAACG TATGTGGAAA TGTTAAAGCC 
GCACAGTGTC CAGAAACAGC AGGGGCTTGT CAAGTTGACC AGACCGGCAA ATCGTCTTGG 
AATCTCGGCC AGTTCAACTC CAAGCTTTCT TATTATGATG GCATGATTCA GCTGACCTAC 
AGAAACGGCT CTCAGTACAA CAACAAGCAG CACACGCAGC GATCCACACA CATCTCCTTC 
CTCTGTGACC GAGAGGCTGG TCCAGGAAAA CCAGAGTTTC AGGATGAGGA TGAGTACACG 
TATAACTTTA AGTGGTACAC TTCCTACGCC TGTCCAGAGA GATTACATGA GTGTGTCGTG 
ACAGACCCCG AAACTCTTCA ACAGTATGAC CTGTCCAGTC TGTCAGTATC TAATGGCGGC 
AGGAACTGGG AGGTCATGGA CTCGTCTGAC ATCAGCAGCC TGAGGAAATA CTACATCAAT 
GTGTGTCGTC CTCTTAAAGC TGTGCCGGGC TGTGACAGAA GAGCTTCAGT CTGTGAGATG 
AAGTTCGAGG CTGACAGGGA AGGCCTGTCT GAGAAGGTGG AGGTCAGTAA TTTGGGCATT 
GCTAAGAAAG GGCCGGTGAT TGTGGAGCAA AACCAGCTGA TGTTGGAGTA CACCAAGGGC 
TCCATGTGTG AAGCAGATGG AAAGACCACC ACATACACAA CACGCATCCA CTTCGTCTGC 
GCTTCTGGGA CACCACCTTC TGGCCCTCGC TTTGTGGTGA ACCAGAATTG TACTGTTGAT 
TTTGTTTGGG ACACAGAAGC AGCCTGTGCC ATTTCTACTG TCGTGGGGAC AAACCAGACG 
TGCTCCGTGA AAGATCCCAA CACTGGCTTT GAATTCAACC TGCAGCCGCT GGCCACTGAC 
AAAGGATACC GCGTCAACGT TAATGGAAAA GACTTTATTG TGAACATTTG TGGTCCAGTG 
AAGGATTGTG AGACTGCTGG ATCTAAAAAT GTGTCCGGCT GTGAGCTCGA GGATCAGAAG 
CCGGTCAGTC AGGTTGGGGG GGAGCGTTCC CTCCAGTTCT CCACCGATGG GGCTCTCACT 
CTCACATACA AGGGGGTCAT GGACATACCC ACAGGTACCC AAGACACCTT CATTATTAAT 
TTTGTGTGTG ACCAAAAGGC CTCTCCACCC TCCCTGAAGC TTATACAGGA AGAGCTGGGC 
ACAAGTACAC ACGTAATCCA TAATGTGATT TTTGAGCTTG CAACTCCACT AGCCTGTAAA 
CCTGCACCTG TGGACTGCAC AGTCACTGAT TCACAGGGCC GGGAGTATGA CCTGGGTGAC 
CTCAGTCTGG ATGAGAAGTC ATATGTTCCT CTGGACACCT CGGATCAGGC CCGGTTTCAG 
AAGTTTTACG TAAATGTGTG TAAACCACTG CCTCGTGTTC AGGGCTGCCC TGCTGGTGCA 
ATTGGAGCCT GCGGACAAAT CAACAGCAGT TTTGTAAATT TGGGATACGT GCAGTCAAAC 
CTCCAGGCCG CAGCAGACGG CTCCATCAGT ATTGTTTATC TGAACGGGGA CAAGTGTGGG 
ACATCAGGAC GTTACTCCAC CAGAATCATC TTCCAGTGTG ATGACAGTCC TGGTGCACCG 
ATGTTCGATC GCAAAGATGG CTGTGAGTTT GTTTTCATCT GGAGAACCTC AGAAGCTTGC 
CCAATCAAAA GAGTCCATGG TGAAAACTGC AAAGTCACAG ACCCCAAGAG CGGTTATGAG 
TACAATCTCA CACCGTTGGC TGGACAGGAC TATGAAGTGA AGAGTTCGAC CTATGAGTAT 
CATTTCGCAG TCTGTGGCCC AATTACAACC TCTGTCTGCC TTCACGATGC CAGCCAATCT 
GTGTCCTCCT GTCAGGTCGA GAACCAAAAG CACAGGATTG CAGGAATAGC CAATCAAAAC 
CTCACGTTTG ACGATGGGAT CATTATGATC AACTATACAA ACGGGGAAAC ATGTCACAAG 
ATTTACGAGC GGTCCACAGC TATTCTATTC TCCTGTGACC ACAGCCGGAA TCCTGGTAAG 
CCAGATTTCA TAAAGGAGAC CGCAGACTGC ACATATCTGT TTGAGTGGCA CACAGCTTTG 
GCCTGCCCTT CCTTTAAAAC CACAACCTGC TCCTACAATG ACGGCAGTGG CCACTCCTAC 
GATCTCTCCT CATTGGCCCT GCACAAATCT AATTGGATTG TTGTGCCAGA ATCATCGAAT 
CAGAAGCAGC GTTACTATAT CAATGTGTGC AAGTCTCTGG TCCCTCAGAC AGGTTTGTGG 
AGCTGCCCGT CCAGTGCTGC AGCCTGTCTG AAGGATGGGG ACGAGTATGT GAACCTGGGG 
GAGGCGGAGT CTGGTCCACA GTGGGATAAA AACGTCCTGG TTCTGAAGTA TACCAACGGA 
AAAGCCTGTC CTGATGGCAA GAGAAACCGG ACCACCATTA TTCGCTTCAA ATGCAATCCA 
GACAAAGTGG ATTCTGAACC CACTCTGATC ACAGCTCTGG AAAACTGTGT CTACAGCTTC 
GTATGGTTCA CAGCCGCAGC CTGTCCTCTC AACAGCACTG AGCATGGAGA CTGTAAAGTC 
ACAAATCCTG CTACAGGTCA TCTGTTTGAT CTTAATGCGC TCAGTCGGGC GGGTGGATAT 
ACGGTTTATG ATCCTGAATC ACACAGAAAG ATGTTCAGAC TGAATGTGTG TGGTGAAATA 
ATCAATGCTG GTTGTGCTAC TGGAACAGGC GTGTGCATCA AGGACAATCA GATGGCCATA 
AGTGCTGGAA AAGCCTCAAG AAAGCTGGTT TATAAGAATC AGGTGGTGGA GCTCAGTTAT 
GAGGACGGGG ATGCATGCTC TACAAATTCC CGCAAACACA AGAGCATCTT TAGCTTCGTC 
TGCAAGTCTG AAGGAGGAGG AACTGATGGA CCCGTGCTGG TTTACTCAGA CGACACAACC 
TGCACTCACT TCTTCACCTG GCACACTCCT CTAGTCTGCG AACAACAGGT GAAGTGCTCT 
GTTTGGAACG GTACCAACCA GATTGACCTC AGTCCTCTAA TACACCTAAC CGGTTACTAC 
ACTGCTATAG ATGAGGATGT GGACCGGGAC AAATCACCTG ACTTCTACAT TAACATCTGC 
CAGCCCCTCA ACCCCATCCC AAGGGTCAAC TGTCCTCCAG GGGCCGCTGT GTGCATGGAC 
CCTGTGGATG GAGAACCAAT TGACATTGGA CGAATCACAT CTCCTCCTCG CTATAACAGT 
GAATCTAAAG AGGTGGAGAT CACCTTCAGC AGCACAACTG TCTGCGCCTC TAATCGGACC 
TCCAACTACT CCTCCAAGAT CATTTTCACC TGCCAGAAAG GAGCAGAGCT GGGTTCTCCT 
CGGATGATTC GGGCTCACAG CTGCATGTAT GTGTTTGAAT GGGCCACTCC TGTGGTTTGT 
CCGGAGATCA TCGAGGCGCA GGGCTGCAAT CTGACCGTCT CTCAGCTGCG CTACACCTTT 
GACCTCTCAG CACTGACTGG ACCTGTTCAG GTTCCTGTAA CTTCTGGCTC TTTCAGCATC 
AACGTGTGTG GGACTGTGGC GAACACGACT TGTAAAGACA GTGCAGTGTG TCTGATTTCC 
AGTGGCTCAA GAGCTTCATA CGGGAACGCT AAACTCATGA ACCTGGACTA CAAGAGAGAA 
GAGCAGTCGG TGATAATGCA GTACAGCGGG GGAGACCCCT GTCCTCAAGG TGTGCAAGGG 
AAGCGTCAGT CTTCGATCTT GTTCACTTGT GATCGAGCGG CGGGTCGTGG AAGTCCACAG 
CTGATCAGCG ATACCCAGGG TTGCTCTGCC ACTTTTCAGT GGAGAACCAA TGTGGTTTGT 
CCACCCAGAA AGATGGAGTG CAAACTGGCA GGACAACACC AAACCTACGA CCTGCGCACA 
CTCTCTTCTC TAACAGAGCC CTGGAAGTTC AGCAATGGCC AAGACTCCTA TTATTTTAAT 
CTGTGTCAGG CCATTCACGG AGGTCAGCCG GGCTGTGCTG CGGAGGCTGC TGTGTGCCGT 
AGACTGGCCG GAGGAAAAAC AGAGACTCTG GGCCGGGTTC ACACGCAGAC CATGGAGTTA 
ATCGATGGAA AAATCCTGGT CAATTATTCC ATGGGCGACG AAGTGTGCGG AAACAAGAAA 
CCAGCCAAAA CAGTCATACA GCTGAAGTGT GGAAGTACAT TCGGCCATCC CAAACTACTG 
TTCAAGGAGG AAAAATCCTG TGAGTTCTGG TTTGAGTGGG AAACTCGATC AGCATGTGCT 
GTAAAGCAGG AGGAAGTGGA GATGGTCAAC GGGACCATTA AAGTTCCCAA AACAGGAGCC 
ACGTTCAGCT TGGGAGCCCT TTATTACCGT TTGCACAATG CCACAGGAGA CATCCGTGCC 
AATGGAGACC GCTATATCTA CCACATCCAG CTGTCGGGCA TCACGGACAG CTCCATTTCC 
ATGTGCTTGG GTGCCAATAT CTGCCAAGTC AAGATTAATG ACAGTTACCG CAGGAAAATC 
GGCTCCTCCA GCAAAGCCAA GTATTATATT AAAGGTGGAA ACCTTGACGT CTTGGTTCCG 
TCTGAATCCG TTTGTGGAAG AGACAAAACC AAAACTGTGT CTTCAGCAAT TCTGTTCCAC 
TGTAGCCCAA CGGCGGGAGA GGGCATCCCA GAATTCTTGC TGGAGACCGA TAGCTGCCAG 
TACCTGTTTG TGTGGCACAC CTCCACCGTT TGTGAATTTA TTTCTTCCAG CTCCATAGAC 
AGCACCGATG GAGGGGAGGA GCTTGCTGGA CTTTCTGGGC GGAGTCAGGC TTTAGGGGCG 
GTGCTTAGCC TGCTGTTGAT TGTGCTCACA ATCTGTTTGC TTGTTCTCCT GCTGCACAAA 
CAAGACAGGA GGCAACTTGT AATCCAGAAA GTGTCGAGCT GCTGCAGGAA AGGAAATCCA 
GTATCTTACA AATACTCCAG GGTGAACATG GATGAAGACG GGTGTGAAGA TGAGATGGAG 
TGGCTGATGG AGGAGACCGG GACCCCGACG CGAGAACCAC AGGAGAACGG TCACATCACC 
ACCAAACCGG TCCGTGCAGA CGCCCTGCGC TCCTTCTCCC TGGATGAACA GGACAGCGAA 
GATGAGGTGC TGACAGTCCC CGGGGTCCGA ATGGCCCCGT CATCCTCACG CCTAAAATCG 
GCATTACTCA AAGCAGAGAG CGACGAGGAT CTGGTAGGGC TCCTCGTGGA CACACGGAGC 
AAAGATAAGA GCCGATATGG GGATAAAAAC CAGACGAGAG CCCAGTTAGA TCCTGACGAC 
AGCGACGAGG ATCTGCTGAA GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

igf2r ( NM_001039627.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034716.1 Danio rerio insulin-like growth factor 2 receptor (igf2r), mRNA.

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CloneID	ODa35507
Clone ID Related Accession (Same CDS sequence)	NM_001039627.2
Accession Version	NM_001039627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	cation-independent mannose-6-phosphate receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929119.19. On Jan 20, 2007 this sequence version replaced NM_001039627.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY570286.1, GDQH01006351.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA898400 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCGTG TTGGATTTTG CGGAGTCGCT GCTACTGTTA TTATTAATAC TTTTCTATTT 
CTACAATGCG TGTCGTCAGC GGCGGCTTCG GATGACAGCC TATGGTACCA GGATCTGTGC 
AGTTACAAAT GGGAGGCCAT TGATAAGGAC AACAATGTGA GCTACGCTCT GAAGCTTTGC 
GATTCATCTC CAAAAACAGA ATGTGGAGAA GGAAGTGCGG TCTGTGCCCA CTATCTTACA 
AGTGGGCAGC ATCAGTCAGT GGGTGAGCTC TCTCTGACGA AGGTCTCGCC TGCTGTGCTC 
GATTTCAACA GCTCGAAGAA ATGCGAAGAC AAGAAGAGCA ATGTTCAGAG CAGCATCACC 
TTTCAATGCG GCAAAACAAT GGGCACGCCT GAGTTTGTCA CCGTCTCCGA GTGTGTGCAT 
TACTTTGAGT GGAGGACGTA TGTCGCCTGC AGACGTGATA AATTTAAACC GCATAAAGAG 
GTCCCCTGCT ATGTCTTTGA CTCAGATGGA AAGAAGCATG ACCTCAATCC GCTGATAAAA 
CTCTCAGATG GCTACTTAGT TGACGACCCG GATGACGAGA TAGATTTCTA CATTAATATA 
TGCAGAAGTC TAAATCGTGC TGGAAGCACC TGTACTGAAG GATCTGCCGC CTGTCTCACT 
CACACCTCTG GTAAAGGCTC ATTTGACATG GGTCAACCTG TTCACCAGCT CGAACTGCTC 
TCTAATGACA AACTAAGACT TCGATACGAG GGAGTCAAAG AAAGCCCAGA CTTCTGTGAT 
GGCCACAGTC CAGCAGTGAC CATTACATTT ATTTGTCCCT CCACAAGGCA GACGGGAAGT 
AACCCTAAGA TGATCAGCAG CACGAACTGT CGATATGAGA TTGAGTGGGT GACGGAGTAC 
GCCTGCCATA GAGATTACCT GGAGAGTCAC GACTGCAAAC TGACCAGCAC TCAGCATGAC 
ATCTCCATCG ACCTCACGCA CCTGACGCAC GGCTCCACAG ATACACCATA CATGGCTGAG 
GCCAAAAATG GCAAGGACAC CTACTTTTTC TATCTGAACG TGTGTGGAGG GACCAATGCT 
GGACAGTGTT TTGACGACAA AGGATACATC TCTGCCTGCC AATTCAAAGA GAATGGCGAT 
GTAAAGAAGA TTGCTGGAAG ATACCAGAAC CAAACCCTGA GATATTCAGA TGGTGATCTC 
ACATTAATCT ACCCAGACGG CAGCAGATGC AGCACTGGAT TCCAGCGCAT GACTATTATT 
AACTTTGAGT GCAATGCAAC TGCTGGGAAC GGTCAGCCGG TTTTTACAGG AGAGTCTGAC 
TGCACGTATT ACTTTGACTG GCAGACGTCG TACGCATGTG TGGAGAAGGA AGACCTGCTG 
TGTCGAGTCA CAGACCACAA GAAGAGATAT GATTTATCTC CTCTTACACG ATTCCCAGAA 
TCGGAGGGAT CTCAGAATTG GGTGGCGGTG AACGCAAAAG CAGCAGAGTC TGATAAAAAG 
CGCATTTATA TGAATGTCTG TCATAAAATC ATCCAGGATG GGGTGGCCAG TTCTTGCCCT 
GAGGACTCTG CAATATGTGC CATAGGAAAT GGGACAGCAA AGAATCTGGG CAAATTCCTG 
AAATCTCCTG AAAAGGCAAC CGAGGGTGAC GACATCAGAC TGATTTACAC AGACGGAGAC 
AAGTGTAGAG AGAAGATGAA GGTGAAGAGC ATCATCACTC TGAAGTGTAA GCCTGGAGAT 
GTGGAAAGTC CTCCAGTCCT CAGGAGTGTG TCCAGCGACG GCTGCATCTA TGAGTTTGAG 
TGGTTTACCG CTTCAGCCTG TGTCCTGTCC AAAACTGAAG GAGACAACTG CAAGGTGGAG 
GACATGCAAA CAGGAATTTC GTTTGATTTG TCTCCGCTTC AGAAATCTAA TGGAGGCTTT 
TATAACATGT CCAGTAAGAA GTATGACTAC TTCATAAACG TATGTGGAAA TGTTAAAGCC 
GCACAGTGTC CAGAAACAGC AGGGGCTTGT CAAGTTGACC AGACCGGCAA ATCGTCTTGG 
AATCTCGGCC AGTTCAACTC CAAGCTTTCT TATTATGATG GCATGATTCA GCTGACCTAC 
AGAAACGGCT CTCAGTACAA CAACAAGCAG CACACGCAGC GATCCACACA CATCTCCTTC 
CTCTGTGACC GAGAGGCTGG TCCAGGAAAA CCAGAGTTTC AGGATGAGGA TGAGTACACG 
TATAACTTTA AGTGGTACAC TTCCTACGCC TGTCCAGAGA GATTACATGA GTGTGTCGTG 
ACAGACCCCG AAACTCTTCA ACAGTATGAC CTGTCCAGTC TGTCAGTATC TAATGGCGGC 
AGGAACTGGG AGGTCATGGA CTCGTCTGAC ATCAGCAGCC TGAGGAAATA CTACATCAAT 
GTGTGTCGTC CTCTTAAAGC TGTGCCGGGC TGTGACAGAA GAGCTTCAGT CTGTGAGATG 
AAGTTCGAGG CTGACAGGGA AGGCCTGTCT GAGAAGGTGG AGGTCAGTAA TTTGGGCATT 
GCTAAGAAAG GGCCGGTGAT TGTGGAGCAA AACCAGCTGA TGTTGGAGTA CACCAAGGGC 
TCCATGTGTG AAGCAGATGG AAAGACCACC ACATACACAA CACGCATCCA CTTCGTCTGC 
GCTTCTGGGA CACCACCTTC TGGCCCTCGC TTTGTGGTGA ACCAGAATTG TACTGTTGAT 
TTTGTTTGGG ACACAGAAGC AGCCTGTGCC ATTTCTACTG TCGTGGGGAC AAACCAGACG 
TGCTCCGTGA AAGATCCCAA CACTGGCTTT GAATTCAACC TGCAGCCGCT GGCCACTGAC 
AAAGGATACC GCGTCAACGT TAATGGAAAA GACTTTATTG TGAACATTTG TGGTCCAGTG 
AAGGATTGTG AGACTGCTGG ATCTAAAAAT GTGTCCGGCT GTGAGCTCGA GGATCAGAAG 
CCGGTCAGTC AGGTTGGGGG GGAGCGTTCC CTCCAGTTCT CCACCGATGG GGCTCTCACT 
CTCACATACA AGGGGGTCAT GGACATACCC ACAGGTACCC AAGACACCTT CATTATTAAT 
TTTGTGTGTG ACCAAAAGGC CTCTCCACCC TCCCTGAAGC TTATACAGGA AGAGCTGGGC 
ACAAGTACAC ACGTAATCCA TAATGTGATT TTTGAGCTTG CAACTCCACT AGCCTGTAAA 
CCTGCACCTG TGGACTGCAC AGTCACTGAT TCACAGGGCC GGGAGTATGA CCTGGGTGAC 
CTCAGTCTGG ATGAGAAGTC ATATGTTCCT CTGGACACCT CGGATCAGGC CCGGTTTCAG 
AAGTTTTACG TAAATGTGTG TAAACCACTG CCTCGTGTTC AGGGCTGCCC TGCTGGTGCA 
ATTGGAGCCT GCGGACAAAT CAACAGCAGT TTTGTAAATT TGGGATACGT GCAGTCAAAC 
CTCCAGGCCG CAGCAGACGG CTCCATCAGT ATTGTTTATC TGAACGGGGA CAAGTGTGGG 
ACATCAGGAC GTTACTCCAC CAGAATCATC TTCCAGTGTG ATGACAGTCC TGGTGCACCG 
ATGTTCGATC GCAAAGATGG CTGTGAGTTT GTTTTCATCT GGAGAACCTC AGAAGCTTGC 
CCAATCAAAA GAGTCCATGG TGAAAACTGC AAAGTCACAG ACCCCAAGAG CGGTTATGAG 
TACAATCTCA CACCGTTGGC TGGACAGGAC TATGAAGTGA AGAGTTCGAC CTATGAGTAT 
CATTTCGCAG TCTGTGGCCC AATTACAACC TCTGTCTGCC TTCACGATGC CAGCCAATCT 
GTGTCCTCCT GTCAGGTCGA GAACCAAAAG CACAGGATTG CAGGAATAGC CAATCAAAAC 
CTCACGTTTG ACGATGGGAT CATTATGATC AACTATACAA ACGGGGAAAC ATGTCACAAG 
ATTTACGAGC GGTCCACAGC TATTCTATTC TCCTGTGACC ACAGCCGGAA TCCTGGTAAG 
CCAGATTTCA TAAAGGAGAC CGCAGACTGC ACATATCTGT TTGAGTGGCA CACAGCTTTG 
GCCTGCCCTT CCTTTAAAAC CACAACCTGC TCCTACAATG ACGGCAGTGG CCACTCCTAC 
GATCTCTCCT CATTGGCCCT GCACAAATCT AATTGGATTG TTGTGCCAGA ATCATCGAAT 
CAGAAGCAGC GTTACTATAT CAATGTGTGC AAGTCTCTGG TCCCTCAGAC AGGTTTGTGG 
AGCTGCCCGT CCAGTGCTGC AGCCTGTCTG AAGGATGGGG ACGAGTATGT GAACCTGGGG 
GAGGCGGAGT CTGGTCCACA GTGGGATAAA AACGTCCTGG TTCTGAAGTA TACCAACGGA 
AAAGCCTGTC CTGATGGCAA GAGAAACCGG ACCACCATTA TTCGCTTCAA ATGCAATCCA 
GACAAAGTGG ATTCTGAACC CACTCTGATC ACAGCTCTGG AAAACTGTGT CTACAGCTTC 
GTATGGTTCA CAGCCGCAGC CTGTCCTCTC AACAGCACTG AGCATGGAGA CTGTAAAGTC 
ACAAATCCTG CTACAGGTCA TCTGTTTGAT CTTAATGCGC TCAGTCGGGC GGGTGGATAT 
ACGGTTTATG ATCCTGAATC ACACAGAAAG ATGTTCAGAC TGAATGTGTG TGGTGAAATA 
ATCAATGCTG GTTGTGCTAC TGGAACAGGC GTGTGCATCA AGGACAATCA GATGGCCATA 
AGTGCTGGAA AAGCCTCAAG AAAGCTGGTT TATAAGAATC AGGTGGTGGA GCTCAGTTAT 
GAGGACGGGG ATGCATGCTC TACAAATTCC CGCAAACACA AGAGCATCTT TAGCTTCGTC 
TGCAAGTCTG AAGGAGGAGG AACTGATGGA CCCGTGCTGG TTTACTCAGA CGACACAACC 
TGCACTCACT TCTTCACCTG GCACACTCCT CTAGTCTGCG AACAACAGGT GAAGTGCTCT 
GTTTGGAACG GTACCAACCA GATTGACCTC AGTCCTCTAA TACACCTAAC CGGTTACTAC 
ACTGCTATAG ATGAGGATGT GGACCGGGAC AAATCACCTG ACTTCTACAT TAACATCTGC 
CAGCCCCTCA ACCCCATCCC AAGGGTCAAC TGTCCTCCAG GGGCCGCTGT GTGCATGGAC 
CCTGTGGATG GAGAACCAAT TGACATTGGA CGAATCACAT CTCCTCCTCG CTATAACAGT 
GAATCTAAAG AGGTGGAGAT CACCTTCAGC AGCACAACTG TCTGCGCCTC TAATCGGACC 
TCCAACTACT CCTCCAAGAT CATTTTCACC TGCCAGAAAG GAGCAGAGCT GGGTTCTCCT 
CGGATGATTC GGGCTCACAG CTGCATGTAT GTGTTTGAAT GGGCCACTCC TGTGGTTTGT 
CCGGAGATCA TCGAGGCGCA GGGCTGCAAT CTGACCGTCT CTCAGCTGCG CTACACCTTT 
GACCTCTCAG CACTGACTGG ACCTGTTCAG GTTCCTGTAA CTTCTGGCTC TTTCAGCATC 
AACGTGTGTG GGACTGTGGC GAACACGACT TGTAAAGACA GTGCAGTGTG TCTGATTTCC 
AGTGGCTCAA GAGCTTCATA CGGGAACGCT AAACTCATGA ACCTGGACTA CAAGAGAGAA 
GAGCAGTCGG TGATAATGCA GTACAGCGGG GGAGACCCCT GTCCTCAAGT AACAGATAAA 
CATGAAGTAT GCGCTTTTCC CTTTATGATA TTGAGTAACA ATTACACTGA ATGCACAACT 
GAAAGCAGGA CTGATGGCAG GAAGTGGTGC GCAACTACAA ACGACTATAA CAAAGATAAG 
AAATGGGGCT TTTGTACTGA CGGTGTGCAA GGGAAGCGTC AGTCTTCGAT CTTGTTCACT 
TGTGATCGAG CGGCGGGTCG TGGAAGTCCA CAGCTGATCA GCGATACCCA GGGTTGCTCT 
GCCACTTTTC AGTGGAGAAC CAATGTGGTT TGTCCACCCA GAAAGATGGA GTGCAAACTG 
GCAGGACAAC ACCAAACCTA CGACCTGCGC ACACTCTCTT CTCTAACAGA GCCCTGGAAG 
TTCAGCAATG GCCAAGACTC CTATTATTTT AATCTGTGTC AGGCCATTCA CGGAGGTCAG 
CCGGGCTGTG CTGCGGAGGC TGCTGTGTGC CGTAGACTGG CCGGAGGAAA AACAGAGACT 
CTGGGCCGGG TTCACACGCA GACCATGGAG TTAATCGATG GAAAAATCCT GGTCAATTAT 
TCCATGGGCG ACGAAGTGTG CGGAAACAAG AAACCAGCCA AAACAGTCAT ACAGCTGAAG 
TGTGGAAGTA CATTCGGCCA TCCCAAACTA CTGTTCAAGG AGGAAAAATC CTGTGAGTTC 
TGGTTTGAGT GGGAAACTCG ATCAGCATGT GCTGTAAAGC AGGAGGAAGT GGAGATGGTC 
AACGGGACCA TTAAAGTTCC CAAAACAGGA GCCACGTTCA GCTTGGGAGC CCTTTATTAC 
CGTTTGCACA ATGCCACAGG AGACATCCGT GCCAATGGAG ACCGCTATAT CTACCACATC 
CAGCTGTCGG GCATCACGGA CAGCTCCATT TCCATGTGCT TGGGTGCCAA TATCTGCCAA 
GTCAAGATTA ATGACAGTTA CCGCAGGAAA ATCGGCTCCT CCAGCAAAGC CAAGTATTAT 
ATTAAAGGTG GAAACCTTGA CGTCTTGGTT CCGTCTGAAT CCGTTTGTGG AAGAGACAAA 
ACCAAAACTG TGTCTTCAGC AATTCTGTTC CACTGTAGCC CAACGGCGGG AGAGGGCATC 
CCAGAATTCT TGCTGGAGAC CGATAGCTGC CAGTACCTGT TTGTGTGGCA CACCTCCACC 
GTTTGTGAAT TTATTTCTTC CAGCTCCATA GACAGCACCG ATGGAGGGGA GGAGCTTGCT 
GGACTTTCTG GGCGGAGTCA GGCTTTAGGG GCGGTGCTTA GCCTGCTGTT GATTGTGCTC 
ACAATCTGTT TGCTTGTTCT CCTGCTGCAC AAACAAGACA GGAGGCAACT TGTAATCCAG 
AAAGTGTCGA GCTGCTGCAG GAAAGGAAAT CCAGTATCTT ACAAATACTC CAGGGTGAAC 
ATGGATGAAG ACGGGTGTGA AGATGAGATG GAGTGGCTGA TGGAGGAGAC CGGGACCCCG 
ACGCGAGAAC CACAGGAGAA CGGTCACATC ACCACCAAAC CGGTCCGTGC AGACGCCCTG 
CGCTCCTTCT CCCTGGATGA ACAGGACAGC GAAGATGAGG TGCTGACAGT CCCCGGGGTC 
CGAATGGCCC CGTCATCCTC ACGCCTAAAA TCGGCATTAC TCAAAGCAGA GAGCGACGAG 
GATCTGGTAG GGCTCCTCGT GGACACACGG AGCAAAGATA AGAGCCGATA TGGGGATAAA 
AACCAGACGA GAGCCCAGTT AGATCCTGAC GACAGCGACG AGGATCTGCT GAAGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034716.1
CDS	1..7380
Translation	MGRVGFCGVAATVIINTFLFLQCVSSAAASDDSLWYQDLCSYKWEAIDKDNNVSYALKLCDSSPKTECGEGSAVCAHYLTSGQHQSVGELSLTKVSPAVLDFNSSKKCEDKKSNVQSSITFQCGKTMGTPEFVTVSECVHYFEWRTYVACRRDKFKPHKEVPCYVFDSDGKKHDLNPLIKLSDGYLVDDPDDEIDFYINICRSLNRAGSTCTEGSAACLTHTSGKGSFDMGQPVHQLELLSNDKLRLRYEGVKESPDFCDGHSPAVTITFICPSTRQTGSNPKMISSTNCRYEIEWVTEYACHRDYLESHDCKLTSTQHDISIDLTHLTHGSTDTPYMAEAKNGKDTYFFYLNVCGGTNAGQCFDDKGYISACQFKENGDVKKIAGRYQNQTLRYSDGDLTLIYPDGSRCSTGFQRMTIINFECNATAGNGQPVFTGESDCTYYFDWQTSYACVEKEDLLCRVTDHKKRYDLSPLTRFPESEGSQNWVAVNAKAAESDKKRIYMNVCHKIIQDGVASSCPEDSAICAIGNGTAKNLGKFLKSPEKATEGDDIRLIYTDGDKCREKMKVKSIITLKCKPGDVESPPVLRSVSSDGCIYEFEWFTASACVLSKTEGDNCKVEDMQTGISFDLSPLQKSNGGFYNMSSKKYDYFINVCGNVKAAQCPETAGACQVDQTGKSSWNLGQFNSKLSYYDGMIQLTYRNGSQYNNKQHTQRSTHISFLCDREAGPGKPEFQDEDEYTYNFKWYTSYACPERLHECVVTDPETLQQYDLSSLSVSNGGRNWEVMDSSDISSLRKYYINVCRPLKAVPGCDRRASVCEMKFEADREGLSEKVEVSNLGIAKKGPVIVEQNQLMLEYTKGSMCEADGKTTTYTTRIHFVCASGTPPSGPRFVVNQNCTVDFVWDTEAACAISTVVGTNQTCSVKDPNTGFEFNLQPLATDKGYRVNVNGKDFIVNICGPVKDCETAGSKNVSGCELEDQKPVSQVGGERSLQFSTDGALTLTYKGVMDIPTGTQDTFIINFVCDQKASPPSLKLIQEELGTSTHVIHNVIFELATPLACKPAPVDCTVTDSQGREYDLGDLSLDEKSYVPLDTSDQARFQKFYVNVCKPLPRVQGCPAGAIGACGQINSSFVNLGYVQSNLQAAADGSISIVYLNGDKCGTSGRYSTRIIFQCDDSPGAPMFDRKDGCEFVFIWRTSEACPIKRVHGENCKVTDPKSGYEYNLTPLAGQDYEVKSSTYEYHFAVCGPITTSVCLHDASQSVSSCQVENQKHRIAGIANQNLTFDDGIIMINYTNGETCHKIYERSTAILFSCDHSRNPGKPDFIKETADCTYLFEWHTALACPSFKTTTCSYNDGSGHSYDLSSLALHKSNWIVVPESSNQKQRYYINVCKSLVPQTGLWSCPSSAAACLKDGDEYVNLGEAESGPQWDKNVLVLKYTNGKACPDGKRNRTTIIRFKCNPDKVDSEPTLITALENCVYSFVWFTAAACPLNSTEHGDCKVTNPATGHLFDLNALSRAGGYTVYDPESHRKMFRLNVCGEIINAGCATGTGVCIKDNQMAISAGKASRKLVYKNQVVELSYEDGDACSTNSRKHKSIFSFVCKSEGGGTDGPVLVYSDDTTCTHFFTWHTPLVCEQQVKCSVWNGTNQIDLSPLIHLTGYYTAIDEDVDRDKSPDFYINICQPLNPIPRVNCPPGAAVCMDPVDGEPIDIGRITSPPRYNSESKEVEITFSSTTVCASNRTSNYSSKIIFTCQKGAELGSPRMIRAHSCMYVFEWATPVVCPEIIEAQGCNLTVSQLRYTFDLSALTGPVQVPVTSGSFSINVCGTVANTTCKDSAVCLISSGSRASYGNAKLMNLDYKREEQSVIMQYSGGDPCPQVTDKHEVCAFPFMILSNNYTECTTESRTDGRKWCATTNDYNKDKKWGFCTDGVQGKRQSSILFTCDRAAGRGSPQLISDTQGCSATFQWRTNVVCPPRKMECKLAGQHQTYDLRTLSSLTEPWKFSNGQDSYYFNLCQAIHGGQPGCAAEAAVCRRLAGGKTETLGRVHTQTMELIDGKILVNYSMGDEVCGNKKPAKTVIQLKCGSTFGHPKLLFKEEKSCEFWFEWETRSACAVKQEEVEMVNGTIKVPKTGATFSLGALYYRLHNATGDIRANGDRYIYHIQLSGITDSSISMCLGANICQVKINDSYRRKIGSSSKAKYYIKGGNLDVLVPSESVCGRDKTKTVSSAILFHCSPTAGEGIPEFLLETDSCQYLFVWHTSTVCEFISSSSIDSTDGGEELAGLSGRSQALGAVLSLLLIVLTICLLVLLLHKQDRRQLVIQKVSSCCRKGNPVSYKYSRVNMDEDGCEDEMEWLMEETGTPTREPQENGHITTKPVRADALRSFSLDEQDSEDEVLTVPGVRMAPSSSRLKSALLKAESDEDLVGLLVDTRSKDKSRYGDKNQTRAQLDPDDSDEDLLKV

Target ORF information:

RefSeq Version	NM_001039627.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio insulin-like growth factor 2 receptor (igf2r), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039627.2

ORF Insert Sequence:

ATGGGTCGTG TTGGATTTTG CGGAGTCGCT GCTACTGTTA TTATTAATAC TTTTCTATTT 
CTACAATGCG TGTCGTCAGC GGCGGCTTCG GATGACAGCC TATGGTACCA GGATCTGTGC 
AGTTACAAAT GGGAGGCCAT TGATAAGGAC AACAATGTGA GCTACGCTCT GAAGCTTTGC 
GATTCATCTC CAAAAACAGA ATGTGGAGAA GGAAGTGCGG TCTGTGCCCA CTATCTTACA 
AGTGGGCAGC ATCAGTCAGT GGGTGAGCTC TCTCTGACGA AGGTCTCGCC TGCTGTGCTC 
GATTTCAACA GCTCGAAGAA ATGCGAAGAC AAGAAGAGCA ATGTTCAGAG CAGCATCACC 
TTTCAATGCG GCAAAACAAT GGGCACGCCT GAGTTTGTCA CCGTCTCCGA GTGTGTGCAT 
TACTTTGAGT GGAGGACGTA TGTCGCCTGC AGACGTGATA AATTTAAACC GCATAAAGAG 
GTCCCCTGCT ATGTCTTTGA CTCAGATGGA AAGAAGCATG ACCTCAATCC GCTGATAAAA 
CTCTCAGATG GCTACTTAGT TGACGACCCG GATGACGAGA TAGATTTCTA CATTAATATA 
TGCAGAAGTC TAAATCGTGC TGGAAGCACC TGTACTGAAG GATCTGCCGC CTGTCTCACT 
CACACCTCTG GTAAAGGCTC ATTTGACATG GGTCAACCTG TTCACCAGCT CGAACTGCTC 
TCTAATGACA AACTAAGACT TCGATACGAG GGAGTCAAAG AAAGCCCAGA CTTCTGTGAT 
GGCCACAGTC CAGCAGTGAC CATTACATTT ATTTGTCCCT CCACAAGGCA GACGGGAAGT 
AACCCTAAGA TGATCAGCAG CACGAACTGT CGATATGAGA TTGAGTGGGT GACGGAGTAC 
GCCTGCCATA GAGATTACCT GGAGAGTCAC GACTGCAAAC TGACCAGCAC TCAGCATGAC 
ATCTCCATCG ACCTCACGCA CCTGACGCAC GGCTCCACAG ATACACCATA CATGGCTGAG 
GCCAAAAATG GCAAGGACAC CTACTTTTTC TATCTGAACG TGTGTGGAGG GACCAATGCT 
GGACAGTGTT TTGACGACAA AGGATACATC TCTGCCTGCC AATTCAAAGA GAATGGCGAT 
GTAAAGAAGA TTGCTGGAAG ATACCAGAAC CAAACCCTGA GATATTCAGA TGGTGATCTC 
ACATTAATCT ACCCAGACGG CAGCAGATGC AGCACTGGAT TCCAGCGCAT GACTATTATT 
AACTTTGAGT GCAATGCAAC TGCTGGGAAC GGTCAGCCGG TTTTTACAGG AGAGTCTGAC 
TGCACGTATT ACTTTGACTG GCAGACGTCG TACGCATGTG TGGAGAAGGA AGACCTGCTG 
TGTCGAGTCA CAGACCACAA GAAGAGATAT GATTTATCTC CTCTTACACG ATTCCCAGAA 
TCGGAGGGAT CTCAGAATTG GGTGGCGGTG AACGCAAAAG CAGCAGAGTC TGATAAAAAG 
CGCATTTATA TGAATGTCTG TCATAAAATC ATCCAGGATG GGGTGGCCAG TTCTTGCCCT 
GAGGACTCTG CAATATGTGC CATAGGAAAT GGGACAGCAA AGAATCTGGG CAAATTCCTG 
AAATCTCCTG AAAAGGCAAC CGAGGGTGAC GACATCAGAC TGATTTACAC AGACGGAGAC 
AAGTGTAGAG AGAAGATGAA GGTGAAGAGC ATCATCACTC TGAAGTGTAA GCCTGGAGAT 
GTGGAAAGTC CTCCAGTCCT CAGGAGTGTG TCCAGCGACG GCTGCATCTA TGAGTTTGAG 
TGGTTTACCG CTTCAGCCTG TGTCCTGTCC AAAACTGAAG GAGACAACTG CAAGGTGGAG 
GACATGCAAA CAGGAATTTC GTTTGATTTG TCTCCGCTTC AGAAATCTAA TGGAGGCTTT 
TATAACATGT CCAGTAAGAA GTATGACTAC TTCATAAACG TATGTGGAAA TGTTAAAGCC 
GCACAGTGTC CAGAAACAGC AGGGGCTTGT CAAGTTGACC AGACCGGCAA ATCGTCTTGG 
AATCTCGGCC AGTTCAACTC CAAGCTTTCT TATTATGATG GCATGATTCA GCTGACCTAC 
AGAAACGGCT CTCAGTACAA CAACAAGCAG CACACGCAGC GATCCACACA CATCTCCTTC 
CTCTGTGACC GAGAGGCTGG TCCAGGAAAA CCAGAGTTTC AGGATGAGGA TGAGTACACG 
TATAACTTTA AGTGGTACAC TTCCTACGCC TGTCCAGAGA GATTACATGA GTGTGTCGTG 
ACAGACCCCG AAACTCTTCA ACAGTATGAC CTGTCCAGTC TGTCAGTATC TAATGGCGGC 
AGGAACTGGG AGGTCATGGA CTCGTCTGAC ATCAGCAGCC TGAGGAAATA CTACATCAAT 
GTGTGTCGTC CTCTTAAAGC TGTGCCGGGC TGTGACAGAA GAGCTTCAGT CTGTGAGATG 
AAGTTCGAGG CTGACAGGGA AGGCCTGTCT GAGAAGGTGG AGGTCAGTAA TTTGGGCATT 
GCTAAGAAAG GGCCGGTGAT TGTGGAGCAA AACCAGCTGA TGTTGGAGTA CACCAAGGGC 
TCCATGTGTG AAGCAGATGG AAAGACCACC ACATACACAA CACGCATCCA CTTCGTCTGC 
GCTTCTGGGA CACCACCTTC TGGCCCTCGC TTTGTGGTGA ACCAGAATTG TACTGTTGAT 
TTTGTTTGGG ACACAGAAGC AGCCTGTGCC ATTTCTACTG TCGTGGGGAC AAACCAGACG 
TGCTCCGTGA AAGATCCCAA CACTGGCTTT GAATTCAACC TGCAGCCGCT GGCCACTGAC 
AAAGGATACC GCGTCAACGT TAATGGAAAA GACTTTATTG TGAACATTTG TGGTCCAGTG 
AAGGATTGTG AGACTGCTGG ATCTAAAAAT GTGTCCGGCT GTGAGCTCGA GGATCAGAAG 
CCGGTCAGTC AGGTTGGGGG GGAGCGTTCC CTCCAGTTCT CCACCGATGG GGCTCTCACT 
CTCACATACA AGGGGGTCAT GGACATACCC ACAGGTACCC AAGACACCTT CATTATTAAT 
TTTGTGTGTG ACCAAAAGGC CTCTCCACCC TCCCTGAAGC TTATACAGGA AGAGCTGGGC 
ACAAGTACAC ACGTAATCCA TAATGTGATT TTTGAGCTTG CAACTCCACT AGCCTGTAAA 
CCTGCACCTG TGGACTGCAC AGTCACTGAT TCACAGGGCC GGGAGTATGA CCTGGGTGAC 
CTCAGTCTGG ATGAGAAGTC ATATGTTCCT CTGGACACCT CGGATCAGGC CCGGTTTCAG 
AAGTTTTACG TAAATGTGTG TAAACCACTG CCTCGTGTTC AGGGCTGCCC TGCTGGTGCA 
ATTGGAGCCT GCGGACAAAT CAACAGCAGT TTTGTAAATT TGGGATACGT GCAGTCAAAC 
CTCCAGGCCG CAGCAGACGG CTCCATCAGT ATTGTTTATC TGAACGGGGA CAAGTGTGGG 
ACATCAGGAC GTTACTCCAC CAGAATCATC TTCCAGTGTG ATGACAGTCC TGGTGCACCG 
ATGTTCGATC GCAAAGATGG CTGTGAGTTT GTTTTCATCT GGAGAACCTC AGAAGCTTGC 
CCAATCAAAA GAGTCCATGG TGAAAACTGC AAAGTCACAG ACCCCAAGAG CGGTTATGAG 
TACAATCTCA CACCGTTGGC TGGACAGGAC TATGAAGTGA AGAGTTCGAC CTATGAGTAT 
CATTTCGCAG TCTGTGGCCC AATTACAACC TCTGTCTGCC TTCACGATGC CAGCCAATCT 
GTGTCCTCCT GTCAGGTCGA GAACCAAAAG CACAGGATTG CAGGAATAGC CAATCAAAAC 
CTCACGTTTG ACGATGGGAT CATTATGATC AACTATACAA ACGGGGAAAC ATGTCACAAG 
ATTTACGAGC GGTCCACAGC TATTCTATTC TCCTGTGACC ACAGCCGGAA TCCTGGTAAG 
CCAGATTTCA TAAAGGAGAC CGCAGACTGC ACATATCTGT TTGAGTGGCA CACAGCTTTG 
GCCTGCCCTT CCTTTAAAAC CACAACCTGC TCCTACAATG ACGGCAGTGG CCACTCCTAC 
GATCTCTCCT CATTGGCCCT GCACAAATCT AATTGGATTG TTGTGCCAGA ATCATCGAAT 
CAGAAGCAGC GTTACTATAT CAATGTGTGC AAGTCTCTGG TCCCTCAGAC AGGTTTGTGG 
AGCTGCCCGT CCAGTGCTGC AGCCTGTCTG AAGGATGGGG ACGAGTATGT GAACCTGGGG 
GAGGCGGAGT CTGGTCCACA GTGGGATAAA AACGTCCTGG TTCTGAAGTA TACCAACGGA 
AAAGCCTGTC CTGATGGCAA GAGAAACCGG ACCACCATTA TTCGCTTCAA ATGCAATCCA 
GACAAAGTGG ATTCTGAACC CACTCTGATC ACAGCTCTGG AAAACTGTGT CTACAGCTTC 
GTATGGTTCA CAGCCGCAGC CTGTCCTCTC AACAGCACTG AGCATGGAGA CTGTAAAGTC 
ACAAATCCTG CTACAGGTCA TCTGTTTGAT CTTAATGCGC TCAGTCGGGC GGGTGGATAT 
ACGGTTTATG ATCCTGAATC ACACAGAAAG ATGTTCAGAC TGAATGTGTG TGGTGAAATA 
ATCAATGCTG GTTGTGCTAC TGGAACAGGC GTGTGCATCA AGGACAATCA GATGGCCATA 
AGTGCTGGAA AAGCCTCAAG AAAGCTGGTT TATAAGAATC AGGTGGTGGA GCTCAGTTAT 
GAGGACGGGG ATGCATGCTC TACAAATTCC CGCAAACACA AGAGCATCTT TAGCTTCGTC 
TGCAAGTCTG AAGGAGGAGG AACTGATGGA CCCGTGCTGG TTTACTCAGA CGACACAACC 
TGCACTCACT TCTTCACCTG GCACACTCCT CTAGTCTGCG AACAACAGGT GAAGTGCTCT 
GTTTGGAACG GTACCAACCA GATTGACCTC AGTCCTCTAA TACACCTAAC CGGTTACTAC 
ACTGCTATAG ATGAGGATGT GGACCGGGAC AAATCACCTG ACTTCTACAT TAACATCTGC 
CAGCCCCTCA ACCCCATCCC AAGGGTCAAC TGTCCTCCAG GGGCCGCTGT GTGCATGGAC 
CCTGTGGATG GAGAACCAAT TGACATTGGA CGAATCACAT CTCCTCCTCG CTATAACAGT 
GAATCTAAAG AGGTGGAGAT CACCTTCAGC AGCACAACTG TCTGCGCCTC TAATCGGACC 
TCCAACTACT CCTCCAAGAT CATTTTCACC TGCCAGAAAG GAGCAGAGCT GGGTTCTCCT 
CGGATGATTC GGGCTCACAG CTGCATGTAT GTGTTTGAAT GGGCCACTCC TGTGGTTTGT 
CCGGAGATCA TCGAGGCGCA GGGCTGCAAT CTGACCGTCT CTCAGCTGCG CTACACCTTT 
GACCTCTCAG CACTGACTGG ACCTGTTCAG GTTCCTGTAA CTTCTGGCTC TTTCAGCATC 
AACGTGTGTG GGACTGTGGC GAACACGACT TGTAAAGACA GTGCAGTGTG TCTGATTTCC 
AGTGGCTCAA GAGCTTCATA CGGGAACGCT AAACTCATGA ACCTGGACTA CAAGAGAGAA 
GAGCAGTCGG TGATAATGCA GTACAGCGGG GGAGACCCCT GTCCTCAAGT AACAGATAAA 
CATGAAGTAT GCGCTTTTCC CTTTATGATA TTGAGTAACA ATTACACTGA ATGCACAACT 
GAAAGCAGGA CTGATGGCAG GAAGTGGTGC GCAACTACAA ACGACTATAA CAAAGATAAG 
AAATGGGGCT TTTGTACTGA CGGTGTGCAA GGGAAGCGTC AGTCTTCGAT CTTGTTCACT 
TGTGATCGAG CGGCGGGTCG TGGAAGTCCA CAGCTGATCA GCGATACCCA GGGTTGCTCT 
GCCACTTTTC AGTGGAGAAC CAATGTGGTT TGTCCACCCA GAAAGATGGA GTGCAAACTG 
GCAGGACAAC ACCAAACCTA CGACCTGCGC ACACTCTCTT CTCTAACAGA GCCCTGGAAG 
TTCAGCAATG GCCAAGACTC CTATTATTTT AATCTGTGTC AGGCCATTCA CGGAGGTCAG 
CCGGGCTGTG CTGCGGAGGC TGCTGTGTGC CGTAGACTGG CCGGAGGAAA AACAGAGACT 
CTGGGCCGGG TTCACACGCA GACCATGGAG TTAATCGATG GAAAAATCCT GGTCAATTAT 
TCCATGGGCG ACGAAGTGTG CGGAAACAAG AAACCAGCCA AAACAGTCAT ACAGCTGAAG 
TGTGGAAGTA CATTCGGCCA TCCCAAACTA CTGTTCAAGG AGGAAAAATC CTGTGAGTTC 
TGGTTTGAGT GGGAAACTCG ATCAGCATGT GCTGTAAAGC AGGAGGAAGT GGAGATGGTC 
AACGGGACCA TTAAAGTTCC CAAAACAGGA GCCACGTTCA GCTTGGGAGC CCTTTATTAC 
CGTTTGCACA ATGCCACAGG AGACATCCGT GCCAATGGAG ACCGCTATAT CTACCACATC 
CAGCTGTCGG GCATCACGGA CAGCTCCATT TCCATGTGCT TGGGTGCCAA TATCTGCCAA 
GTCAAGATTA ATGACAGTTA CCGCAGGAAA ATCGGCTCCT CCAGCAAAGC CAAGTATTAT 
ATTAAAGGTG GAAACCTTGA CGTCTTGGTT CCGTCTGAAT CCGTTTGTGG AAGAGACAAA 
ACCAAAACTG TGTCTTCAGC AATTCTGTTC CACTGTAGCC CAACGGCGGG AGAGGGCATC 
CCAGAATTCT TGCTGGAGAC CGATAGCTGC CAGTACCTGT TTGTGTGGCA CACCTCCACC 
GTTTGTGAAT TTATTTCTTC CAGCTCCATA GACAGCACCG ATGGAGGGGA GGAGCTTGCT 
GGACTTTCTG GGCGGAGTCA GGCTTTAGGG GCGGTGCTTA GCCTGCTGTT GATTGTGCTC 
ACAATCTGTT TGCTTGTTCT CCTGCTGCAC AAACAAGACA GGAGGCAACT TGTAATCCAG 
AAAGTGTCGA GCTGCTGCAG GAAAGGAAAT CCAGTATCTT ACAAATACTC CAGGGTGAAC 
ATGGATGAAG ACGGGTGTGA AGATGAGATG GAGTGGCTGA TGGAGGAGAC CGGGACCCCG 
ACGCGAGAAC CACAGGAGAA CGGTCACATC ACCACCAAAC CGGTCCGTGC AGACGCCCTG 
CGCTCCTTCT CCCTGGATGA ACAGGACAGC GAAGATGAGG TGCTGACAGT CCCCGGGGTC 
CGAATGGCCC CGTCATCCTC ACGCCTAAAA TCGGCATTAC TCAAAGCAGA GAGCGACGAG 
GATCTGGTAG GGCTCCTCGT GGACACACGG AGCAAAGATA AGAGCCGATA TGGGGATAAA 
AACCAGACGA GAGCCCAGTT AGATCCTGAC GACAGCGACG AGGATCTGCT GAAGGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish.
Environmental pollution (Barking, Essex : 1987)229470-478(2017 Oct)
Zhu Y,Su G,Yang D,Zhang Y,Yu L,Li Y,Giesy JP,Letcher RJ,Liu C

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Effects of microgravity simulation on zebrafish transcriptomes and bone physiology-exposure starting at 5 days post fertilization.
NPJ microgravity216010(2016)
Aceto J,Nourizadeh-Lillabadi R,Bradamante S,Maier JA,Alestrom P,van Loon JJ,Muller M

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igf2r cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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