fam20b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	fam20b
Entrez Gene ID	557060
Full Name	family with sequence similarity 20, member B (H. sapiens)
Synonyms	si:ch211-195o19.3,zgc:136944
General protein information	Preferred Names family with sequence similarity 20, member B (H. sapiens) Names glycosaminoglycan xylosylkinase unm b1127 unm_b1127
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160420.3	XP_005160477.1	glycosaminoglycan xylosylkinase isoform X1
XM_009294613.2	XP_009292888.1	glycosaminoglycan xylosylkinase isoform X1
XM_009294614.2	XP_009292889.1	glycosaminoglycan xylosylkinase isoform X1
XM_005160420.4	XP_005160477.1	glycosaminoglycan xylosylkinase isoform X1
XM_009294613.3	XP_009292888.1	glycosaminoglycan xylosylkinase isoform X1
XM_009294614.3	XP_009292889.1	glycosaminoglycan xylosylkinase isoform X1
NM_001044818.1	NP_001038283.1	glycosaminoglycan xylosylkinase

Pathways from BioSystems

Homologies

	FAM20B	XP_001115532.1
Mus musculus (house mouse)	Fam20b	NP_663388.1
Danio rerio (zebrafish)	fam20b	NP_001038283.1
Pan troglodytes (chimpanzee)	FAM20B	XP_514027.2
Rattus norvegicus (Norway rat)	Fam20b	NP_001100657.1
Gallus gallus (chicken)	FAM20B	NP_001026439.1
Drosophila melanogaster (fruit fly)	CG3631	NP_996212.1
Homo sapiens (human)	FAM20B	NP_055679.1
Canis lupus familiaris (dog)	FAM20B	XP_005622528.1
Bos taurus (cattle)	FAM20B	NP_001069653.1
Xenopus tropicalis (tropical clawed frog)	fam20b	NP_989211.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001051	XP_318250.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Biswas G, Kinoshita S, Kono T, Hikima J, Sakai M
Marine genomics2225-36(2015 Aug)

Mutations in fam20b and xylt1 reveal that cartilage matrix controls timing of endochondral ossification by inhibiting chondrocyte maturation.
Eames BF, Yan YL, Swartz ME, Levic DS, Knapik EW, Postlethwait JH, Kimmel CB
PLoS genetics7(8)e1002246(2011 Aug)

FAM20: an evolutionarily conserved family of secreted proteins expressed in hematopoietic cells.
Nalbant D, Youn H, Nalbant SI, Sharma S, Cobos E, Beale EG, Du Y, Williams SC
BMC genomics611(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in vivo analyses reveal that fam20b and xylt1 function in cartilage matrix production and ultimately regulate the timing of skeletal development
Title: Mutations in fam20b and xylt1 reveal that cartilage matrix controls timing of endochondral ossification by inhibiting chondrocyte maturation.

The following fam20b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fam20b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00093	NM_001044818.1 Latest version!	Danio rerio FAM20B glycosaminoglycan xylosylkinase (fam20b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa00093	XM_005160420.4 Latest version!	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa00093	XM_009294613.3 Latest version!	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa00093	XM_009294614.3 Latest version!	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa00093	XM_005160420.3	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa00093	XM_009294613.2	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa00093	XM_009294614.2	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fam20b ( NM_001044818.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038283.1 Danio rerio FAM20B glycosaminoglycan xylosylkinase (fam20b), mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	NM_001044818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	glycosaminoglycan xylosylkinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571854.11. On Sep 1, 2006 this sequence version replaced XM_680038.1. ##Evidence-Data-START## Transcript exon combination :: GFIL01010206.1, GDQH01013425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476740, SAMEA4476783 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038283.1
CDS	363..1592
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	NM_001044818.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio FAM20B glycosaminoglycan xylosylkinase (fam20b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044818.1

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_005160420.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160477.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_005160420.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160477.1
CDS	468..1697
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_005160420.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160420.3

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_009294613.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292888.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_009294613.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294613.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292888.1
CDS	295..1524
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_009294613.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294613.2

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_009294614.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292889.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_009294614.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294614.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292889.1
CDS	345..1574
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_009294614.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294614.2

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_005160420.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160477.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_005160420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160420.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160477.1
CDS	465..1694
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_005160420.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160420.4

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_009294613.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292888.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_009294613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292888.1
CDS	334..1563
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_009294613.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294613.3

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam20b ( XM_009294614.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292889.1 Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa00093
Clone ID Related Accession (Same CDS sequence)	XM_005160420.3 , XM_009294613.2 , XM_009294614.2 , XM_005160420.4 , XM_009294613.3 , XM_009294614.3 , NM_001044818.1
Accession Version	XM_009294614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	glycosaminoglycan xylosylkinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292889.1
CDS	384..1613
Translation	MKLKQRVVVLCAVLFLLGLAKVFLLDGGEGSAASRRDLRAFRKMEASLSLAKGARLTHTLQSPWEVAAQWVGPREVYPDETPELAAVLNALATAHVERADVGYKGTQLKALLVLDGGQKVVFKPKRYVRDYVVEGEPYAGYDRHNAEIAAFHLDRILGFRRAPLVVGRFMNLRTEIKPVATDQLLSTFLMHGNNTCFYGKCYYCRETEPACAEGDVMEGSVTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTSIFDYLIGNADRHHYESFQDDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMVRVSTWNRLNLLKGGVLSSAMRQATAHDPAFPVLTGAHLTALDRRLNGVLATVRQCMETQGSENTLIEDRMNLPHP

Target ORF information:

RefSeq Version	XM_009294614.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 20, member B (H. sapiens) (fam20b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294614.3

ORF Insert Sequence:

ATGAAGTTGA AGCAGCGTGT GGTGGTGCTC TGCGCTGTGC TCTTCCTCCT CGGCCTGGCC 
AAGGTCTTCC TACTGGATGG AGGAGAGGGA TCAGCCGCTA GCCGCCGCGA CCTCCGGGCC 
TTCCGAAAGA TGGAGGCCAG CCTTTCCCTG GCCAAAGGAG CTCGCCTCAC ACACACCCTT 
CAGTCGCCCT GGGAAGTGGC TGCCCAGTGG GTGGGTCCGC GAGAGGTTTA CCCAGACGAA 
ACACCTGAGT TAGCTGCTGT GCTGAATGCT CTGGCCACCG CTCATGTGGA GAGGGCAGAC 
GTGGGATATA AAGGAACCCA GTTAAAAGCC CTGCTGGTAC TCGACGGAGG ACAGAAAGTT 
GTGTTTAAGC CCAAGAGGTA CGTTAGAGAC TATGTGGTGG AGGGAGAGCC ATATGCAGGG 
TACGATCGCC ATAATGCAGA AATCGCTGCT TTCCACTTGG ACAGGATTCT GGGTTTTAGG 
AGAGCGCCTC TGGTGGTGGG GAGGTTTATG AATCTGCGCA CGGAGATAAA ACCAGTGGCT 
ACAGATCAAC TGCTGAGCAC TTTCCTGATG CACGGGAATA ATACATGCTT CTATGGCAAG 
TGTTATTACT GTCGTGAGAC GGAGCCAGCG TGCGCAGAAG GGGATGTGAT GGAGGGATCG 
GTGACTTTGT GGCTCCCTGA CGTCTGGCCC TTACAGAAAC ACAGACACCC ATGGGGACGC 
ACATACAGAG AAGGCAAACT CGCCAGGTGG GAGTATGATG AGAGTTACTG CGAGGCTGTA 
AAGAAAATGC CCCCTTACGA TGCTGGGCCG CGACTGCTGG ATGTTATCGA CACTTCCATC 
TTCGATTATC TTATTGGCAA TGCTGACCGC CATCACTACG AGAGCTTCCA GGACGATGGC 
GGAGCAAGTA TGCTCATCCT TCTGGATAAT GCCAAAAGTT TTGGGAACCC GTCTTTGGAT 
GAGAGGAGTA TTCTCGCCCC GCTCTATCAG TGCTGCATGG TCCGCGTGTC CACCTGGAAC 
AGACTGAACC TCCTGAAAGG TGGCGTGTTG AGTTCAGCCA TGCGGCAGGC CACGGCTCAC 
GATCCTGCTT TTCCAGTCTT GACGGGAGCA CATCTCACTG CGCTCGACCG GCGCCTCAAT 
GGAGTATTAG CCACCGTCCG GCAGTGCATG GAGACGCAGG GCTCGGAAAA CACACTGATC 
GAGGACCGGA TGAACCTGCC TCATCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Evolutionary evidence of tumor necrosis factor super family members in the Japanese pufferfish (Takifugu rubripes): Comprehensive genomic identification and expression analysis.
Marine genomics2225-36(2015 Aug)
Biswas G,Kinoshita S,Kono T,Hikima J,Sakai M

Mutations in fam20b and xylt1 reveal that cartilage matrix controls timing of endochondral ossification by inhibiting chondrocyte maturation.
PLoS genetics7(8)e1002246(2011 Aug)
Eames BF,Yan YL,Swartz ME,Levic DS,Knapik EW,Postlethwait JH,Kimmel CB

FAM20: an evolutionarily conserved family of secreted proteins expressed in hematopoietic cells.
BMC genomics611(2005 Jan)
Nalbant D,Youn H,Nalbant SI,Sharma S,Cobos E,Beale EG,Du Y,Williams SC

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fam20b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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