nub1 cDNA ORF clone, Danio rerio(zebrafish)

The following nub1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nub1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33268 NM_001113580.1
Latest version!
Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa28960 XM_005170646.4
Latest version!
Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa42486 XM_009297673.3
Latest version!
Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa42486 XM_009297673.2 Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa28960 XM_005170646.3 Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa33268
    Clone ID Related Accession (Same CDS sequence) NM_001113580.1
    Accession Version NM_001113580.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-27
    Organism Danio rerio(zebrafish)
    Product NEDD8 ultimate buster 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154454.1. On Jan 20, 2008 this sequence version replaced XM_679980.2. ##Evidence-Data-START## Transcript exon combination :: BC154454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505371 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGGAAC AGCACCTACA GGCTCGACTG ATCGCTTTAC TCCGACAGGA CAAAATACAG 
    CTGTGGCTGG AGCCTTACAG GAGCCTCAGA GACCCGGCAG ACCGACTGAA GATCCAGGAA
    CTGGCGGAAA AGTATGCATC AGTCATCCAG TTTCCAGTGG AGGAAGTGAC ATCAGCTCTG
    GAGAGCATCA GAACAGACAC CAGCCAGAAA GATGAAGGAA ATAAAGAGTT TAAGCAGACG
    GCAGTGGCTA CACTGGTGCT CCATCTGCCC AAAGAAGAAG GAGAGACAAA GATTAAGAAG
    GTTAAGTTTA AAACCAAACT GGACATCACC ACACAAGAGC TGAAGACACA GATAAGTCAG
    GAGTTTGGAT TCACATACTT CGATCTGATT TTGTGTGGAA AAATACTGTC TGCAGAGCGA
    AGGCTTGATG AGCAGAAAGT GAAGAACAAC AGTAAGATCA TGGTGATGAA AACGGCTCCT
    CTAGATGAAA AGGCTGAAAT GAAGAAAGAA GAGGAAAAGA CGCGCAGACA GGATGAAGAG
    CTTCAGAAAA CACATAGGGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACAC CATTCTTAGA GATCGCAGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT TCTGGCCATG GGTTTTCATG AGAGAGGTCG AGCTCTGATG
    AAGAAGAGGG ATCACAGTGC TGCTCTTTCT TACCTGCTGC GCGCCAATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGACGTG
    GTGTGGTGTT ACCAGGCTCT GGAGCAGATG GAGTGTCTGG ATGATGCCCA AGAGAGGTTG
    GAGGAAGCAG AGCGGTGTTT CCAGAAGTGT TACGGAGAAA AGCAAAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCATCT ACTGCAGACC
    GTGCTGATGT TCGTCAAAGG CAACGAGAAC CAGGCACTTG ACAAACTCAG AGACGTGGAG
    CATCTGTACA GTCAGCTTAA TCTAGACCCA CAGAAGCTCC AGCAGCTCCA GGATAAGGGT
    TTCTCTGAGC AGGAGGCTCG TCTAGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAAGCA
    GAAAATCTCA TCTCACAGAG AAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGGC GGCGCCTACA GGACATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGGAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATGTTG
    CTGGATGCAG CTGATGCTCA GCATCAAGTA AATCTGAATC AGCTGCTGTC AATGGGCTTC
    CAAAAGGACA TGGCGGACTC TGCGCTGAGG ATGATGAGAG ATGATGTTCA ACAGGCCACA
    CAGCTGCTGC TGGAGAACCA GGCAGTGATT CCTGCTGACC TTCAGTCTCC TTCACCTCCA
    TCATCCTCAT CTGAAGAGCC CAGCACATCC TCGGAGTCCA CCGGCGGCTC CAGTCGTCAG
    GATGAGGATC TGCTGGTGAA CGAGCTGTTG GAGGATATTC CCAAACACGA GGAGGATTAT
    CTGGACCTGA CGCTGGACGA GGAGAAAAAG CTGATAGAGA AGATCAGAGC CGCCCTGGAG
    AAGAAGAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001107052.1
    CDS275..2026
    Translation

    Target ORF information:

    RefSeq Version NM_001113580.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001113580.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAGGAAC AGCACCTACA GGCTCGACTG ATCGCTTTAC TCCGACAGGA CAAAATACAG 
    CTGTGGCTGG AGCCTTACAG GAGCCTCAGA GACCCGGCAG ACCGACTGAA GATCCAGGAA
    CTGGCGGAAA AGTATGCATC AGTCATCCAG TTTCCAGTGG AGGAAGTGAC ATCAGCTCTG
    GAGAGCATCA GAACAGACAC CAGCCAGAAA GATGAAGGAA ATAAAGAGTT TAAGCAGACG
    GCAGTGGCTA CACTGGTGCT CCATCTGCCC AAAGAAGAAG GAGAGACAAA GATTAAGAAG
    GTTAAGTTTA AAACCAAACT GGACATCACC ACACAAGAGC TGAAGACACA GATAAGTCAG
    GAGTTTGGAT TCACATACTT CGATCTGATT TTGTGTGGAA AAATACTGTC TGCAGAGCGA
    AGGCTTGATG AGCAGAAAGT GAAGAACAAC AGTAAGATCA TGGTGATGAA AACGGCTCCT
    CTAGATGAAA AGGCTGAAAT GAAGAAAGAA GAGGAAAAGA CGCGCAGACA GGATGAAGAG
    CTTCAGAAAA CACATAGGGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACAC CATTCTTAGA GATCGCAGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT TCTGGCCATG GGTTTTCATG AGAGAGGTCG AGCTCTGATG
    AAGAAGAGGG ATCACAGTGC TGCTCTTTCT TACCTGCTGC GCGCCAATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGACGTG
    GTGTGGTGTT ACCAGGCTCT GGAGCAGATG GAGTGTCTGG ATGATGCCCA AGAGAGGTTG
    GAGGAAGCAG AGCGGTGTTT CCAGAAGTGT TACGGAGAAA AGCAAAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCATCT ACTGCAGACC
    GTGCTGATGT TCGTCAAAGG CAACGAGAAC CAGGCACTTG ACAAACTCAG AGACGTGGAG
    CATCTGTACA GTCAGCTTAA TCTAGACCCA CAGAAGCTCC AGCAGCTCCA GGATAAGGGT
    TTCTCTGAGC AGGAGGCTCG TCTAGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAAGCA
    GAAAATCTCA TCTCACAGAG AAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGGC GGCGCCTACA GGACATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGGAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATGTTG
    CTGGATGCAG CTGATGCTCA GCATCAAGTA AATCTGAATC AGCTGCTGTC AATGGGCTTC
    CAAAAGGACA TGGCGGACTC TGCGCTGAGG ATGATGAGAG ATGATGTTCA ACAGGCCACA
    CAGCTGCTGC TGGAGAACCA GGCAGTGATT CCTGCTGACC TTCAGTCTCC TTCACCTCCA
    TCATCCTCAT CTGAAGAGCC CAGCACATCC TCGGAGTCCA CCGGCGGCTC CAGTCGTCAG
    GATGAGGATC TGCTGGTGAA CGAGCTGTTG GAGGATATTC CCAAACACGA GGAGGATTAT
    CTGGACCTGA CGCTGGACGA GGAGAAAAAG CTGATAGAGA AGATCAGAGC CGCCCTGGAG
    AAGAAGAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28960
    Clone ID Related Accession (Same CDS sequence) XM_005170646.3 , XM_005170646.4
    Accession Version XM_005170646.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NEDD8 ultimate buster 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCGGCTCC
    AGTCGTCAGG ACGAGGATCT GCTGGTGAAC GAGCTGTTGG AGGATATTCC CAAACACGAG
    GAGGATTATC TGGACCTGAC GCTGGAGGAG GAGAAAGAGC TGATGGACAA GATCAGAACC
    ATCCTGGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170703.1
    CDS265..2019
    Translation

    Target ORF information:

    RefSeq Version XM_005170646.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170646.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCGGCTCC
    AGTCGTCAGG ACGAGGATCT GCTGGTGAAC GAGCTGTTGG AGGATATTCC CAAACACGAG
    GAGGATTATC TGGACCTGAC GCTGGAGGAG GAGAAAGAGC TGATGGACAA GATCAGAACC
    ATCCTGGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa28960
    Clone ID Related Accession (Same CDS sequence) XM_005170646.3 , XM_005170646.4
    Accession Version XM_005170646.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NEDD8 ultimate buster 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCGGCTCC
    AGTCGTCAGG ACGAGGATCT GCTGGTGAAC GAGCTGTTGG AGGATATTCC CAAACACGAG
    GAGGATTATC TGGACCTGAC GCTGGAGGAG GAGAAAGAGC TGATGGACAA GATCAGAACC
    ATCCTGGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005170703.1
    CDS292..2046
    Translation

    Target ORF information:

    RefSeq Version XM_005170646.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005170646.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCGGCTCC
    AGTCGTCAGG ACGAGGATCT GCTGGTGAAC GAGCTGTTGG AGGATATTCC CAAACACGAG
    GAGGATTATC TGGACCTGAC GCTGGAGGAG GAGAAAGAGC TGATGGACAA GATCAGAACC
    ATCCTGGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42486
    Clone ID Related Accession (Same CDS sequence) XM_009297673.2 , XM_009297673.3
    Accession Version XM_009297673.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NEDD8 ultimate buster 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCTCCAGT
    CGTCAGGACG AGGATCTGCT GGTGAACGAG CTGTTGGAGG ATATTCCCAA ACACGAGGAG
    GATTATCTGG ACCTGACGCT GGAGGAGGAG AAAGAGCTGA TGGACAAGAT CAGAACCATC
    CTGGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295948.1
    CDS265..2016
    Translation

    Target ORF information:

    RefSeq Version XM_009297673.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCTCCAGT
    CGTCAGGACG AGGATCTGCT GGTGAACGAG CTGTTGGAGG ATATTCCCAA ACACGAGGAG
    GATTATCTGG ACCTGACGCT GGAGGAGGAG AAAGAGCTGA TGGACAAGAT CAGAACCATC
    CTGGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42486
    Clone ID Related Accession (Same CDS sequence) XM_009297673.2 , XM_009297673.3
    Accession Version XM_009297673.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NEDD8 ultimate buster 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCTCCAGT
    CGTCAGGACG AGGATCTGCT GGTGAACGAG CTGTTGGAGG ATATTCCCAA ACACGAGGAG
    GATTATCTGG ACCTGACGCT GGAGGAGGAG AAAGAGCTGA TGGACAAGAT CAGAACCATC
    CTGGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295948.1
    CDS292..2043
    Translation

    Target ORF information:

    RefSeq Version XM_009297673.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio negative regulator of ubiquitin-like proteins 1 (nub1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297673.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGGAAG AACAACATCT CCAGGCTCGA CTGATCGCTT TACTCCGACA GGACAAAATA 
    CAGCTGTGGC TGGAGCCTTA CAGGAGCCTC AGAGACCCGG CAGACCGACT GAAGATCCAG
    GAACTGGCTG AAAAGTATGC ATCAGTCATT CAGTTTCCAG TGGAGGAAGT GACATCAGCT
    CTGGAGAGCA TCAGAACAGA CACCAGCCGG AAAGATGAAG GAAATAAAGA GTTTAAGGAG
    ACGGCAGTGG CTACGCTGGT GCTCCATCTG CCCAGAGAAG AAGGAGAGAC AAAGATTAAG
    AAGGTTGAGT TAAAAACCAA ACTGGACATC ACCACACAAG AGCTGAAGAA ACAGATAAGT
    CAGGAGTTTG GATTCACACA CTTCAATCTG ATTTGTGGTA AAGTTCTGTC TCCAGAGAGA
    AGGCTTGATG AGCAAAAAGT GAAGAACAAC AGTAAGATCA TGGTGGTGAA AGGGGCTCCT
    CCGGATGCCA AAGCTGAAAT GTACAAAAAC GAAGAGGAGA AGCGCAAACA GGATGAAGAG
    ATTAAGAAAA CACAGAAAGG CTTCCAGATC CTGTCTGAGA GAGATGGGAG TGAAGATCCG
    TCCACCACGC CATTCTTAGA GATCGCGGAT CAGAAAGGAA ATCCACTCCA GATCCCAGAC
    GAGGAGAGGA AGGCTCTGAT CCTGGCCATG GGTTTTCATG AGAAAGGTCG AGCTCTGATG
    AAGAAGAGAG ACCACAGCGC CGCCCTACCT TACCTGGTGC TGGCAGATGA ACAGTTCAAG
    AAGTGCAACT CTGCGATTCT GCACACGGTG GACAATTATG CGGTGCTGCA GCTGGATGTG
    GTGTGGTGTT ACCAGGCTCT GGGGCAGCTG GAGTGTCTGG ATAATGCCCG GCAGAGGCTG
    GAGAAAGCAG AGCAGTGTTT CCAGAAGTGT TACGGAGAGA AGCAGAGCCG CCTGCAACAG
    ATCAAGGGAC ACACCGGAGG AGAAGAAGTG CTGTTTCTGA GGCTCCGTAT ACTGCAGACC
    CTGCTGACAT ACATTGAAGG CCACAAGAAC CAGGCGCTTG GCAAACTCAA AGATGTGGAG
    CATCTGTACG GTGAGCTGAG TCTGGACCCA CAGAAGCTCC AGCAGCTCAA GGAGCTGGGT
    TTCTCTGAGC AGGAGGCTCG TCTGGGTCTG AGAGCCTGCA GAGGAGACGT TTCTGAGGCC
    GCACTGCTCA TCACACAGAG GAAGAAGGAG AAGCAGGTGT TGAAGCTGAA AGAGCAGGAT
    AAGAGGAGAC GGCGCCTGCA GGATATCAAC ACTCTGGTGG AGCTGGGCTT CGAGAGAAGA
    GACGCACAGA GAGCCCTGCA TCAGGCCAGA GGAGACGTGG ACAAGGCCTA CGGGATTCTG
    CTGGATGGGG CTGATGCTCA AAAATCAGAT CGAGATAAAA AGCTGAATGA GCTGCTGTCG
    ATGGGCTTCC AGGAGGACAT GGCGGACTCT GCGCTGAGGA TGACGGGAGA TGATGTTCAA
    CAGGCCACAC AGCTGCTGCT GGAGAACCAG GCAGTGATTC CTGCTGACCT TCAGTCTCCT
    TCACCTCCAT CATCATCATC TGAAGAGCCC AGCACATCCT CGGAGTCCAC CGGCTCCAGT
    CGTCAGGACG AGGATCTGCT GGTGAACGAG CTGTTGGAGG ATATTCCCAA ACACGAGGAG
    GATTATCTGG ACCTGACGCT GGAGGAGGAG AAAGAGCTGA TGGACAAGAT CAGAACCATC
    CTGGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    RFX transcription factors are essential for hearing in mice.
    Nature communications68549(2015 Oct)
    Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R