sema5ba cDNA ORF clone, Danio rerio(zebrafish)

The following sema5ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema5ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa15440 XM_005167866.4
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa15438 XM_679287.6
Latest version!
Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa15438 XM_679287.5 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa15440 XM_005167866.3 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa15440
    Clone ID Related Accession (Same CDS sequence) XM_005167866.3 , XM_005167866.4
    Accession Version XM_005167866.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-5B isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GGGTTCACTC TGCTCCACCT GATAGCGACA GGTGTGTCGT GTTTTCTTGG TGCCGGTCTG
    CTGTCTTTCC TGGTGTATGT TTACTGTCAG CGGTTCCACA AGCCCTCGCA GGAGTCTGCC
    ATCATCCATC CCACCACTCC CAACCACCTC AACTACAAGG GCAACACCAC ACCAAAGAAT
    GAGAAATACA CACCCATGGA GTTCAAGACT CTGAATAAGA ACAATCTCCT GCCAGACGAG
    AGGACAAACT ACTTCCCTTC ACCGCTTCAG CAGACCAACG TCTACACCAC CACGTACTAC
    CCCACAGGAC TGGGCAAATA CGACTACCGG CCGGACTCCT CACCCTCGCC CTGCAGAACC
    TATAACCACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167923.1
    CDS259..3213
    Translation

    Target ORF information:

    RefSeq Version XM_005167866.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GGGTTCACTC TGCTCCACCT GATAGCGACA GGTGTGTCGT GTTTTCTTGG TGCCGGTCTG
    CTGTCTTTCC TGGTGTATGT TTACTGTCAG CGGTTCCACA AGCCCTCGCA GGAGTCTGCC
    ATCATCCATC CCACCACTCC CAACCACCTC AACTACAAGG GCAACACCAC ACCAAAGAAT
    GAGAAATACA CACCCATGGA GTTCAAGACT CTGAATAAGA ACAATCTCCT GCCAGACGAG
    AGGACAAACT ACTTCCCTTC ACCGCTTCAG CAGACCAACG TCTACACCAC CACGTACTAC
    CCCACAGGAC TGGGCAAATA CGACTACCGG CCGGACTCCT CACCCTCGCC CTGCAGAACC
    TATAACCACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15440
    Clone ID Related Accession (Same CDS sequence) XM_005167866.3 , XM_005167866.4
    Accession Version XM_005167866.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GGGTTCACTC TGCTCCACCT GATAGCGACA GGTGTGTCGT GTTTTCTTGG TGCCGGTCTG
    CTGTCTTTCC TGGTGTATGT TTACTGTCAG CGGTTCCACA AGCCCTCGCA GGAGTCTGCC
    ATCATCCATC CCACCACTCC CAACCACCTC AACTACAAGG GCAACACCAC ACCAAAGAAT
    GAGAAATACA CACCCATGGA GTTCAAGACT CTGAATAAGA ACAATCTCCT GCCAGACGAG
    AGGACAAACT ACTTCCCTTC ACCGCTTCAG CAGACCAACG TCTACACCAC CACGTACTAC
    CCCACAGGAC TGGGCAAATA CGACTACCGG CCGGACTCCT CACCCTCGCC CTGCAGAACC
    TATAACCACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005167923.1
    CDS293..3247
    Translation

    Target ORF information:

    RefSeq Version XM_005167866.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005167866.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GGGTTCACTC TGCTCCACCT GATAGCGACA GGTGTGTCGT GTTTTCTTGG TGCCGGTCTG
    CTGTCTTTCC TGGTGTATGT TTACTGTCAG CGGTTCCACA AGCCCTCGCA GGAGTCTGCC
    ATCATCCATC CCACCACTCC CAACCACCTC AACTACAAGG GCAACACCAC ACCAAAGAAT
    GAGAAATACA CACCCATGGA GTTCAAGACT CTGAATAAGA ACAATCTCCT GCCAGACGAG
    AGGACAAACT ACTTCCCTTC ACCGCTTCAG CAGACCAACG TCTACACCAC CACGTACTAC
    CCCACAGGAC TGGGCAAATA CGACTACCGG CCGGACTCCT CACCCTCGCC CTGCAGAACC
    TATAACCACA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15438
    Clone ID Related Accession (Same CDS sequence) XM_679287.6 , XM_679287.5
    Accession Version XM_679287.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product semaphorin-5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679287.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GTTAAAGGCG CCTGGTCTTG CTGGTCATCA TGGTCGCAGT GTTCGGTTCC CTGTGGAGGC
    GGACACTACC AACGAACACG CACATGTACA AGCCCTGCCC CTGCCAATGG AGGGGACATC
    TGCATCGGCT TGCACACAGA GGAGGCTCTT TGCAACACGC ATACTTGTGA TGGTGGCTGG
    ATGGCCTGGT CTGTGTGGTC TGAGTGTGAT GATTCAGGCC TGCAGCTGCG CAGTCGAGTG
    TGTGGAGCTC AATCCATGCC ATGTGCGGGA AACAGCTCCC AGCACAGAGA CTGCAATGAG
    ATCCCAGCCA TTCTTCCGGC ATCCAGCTAT GAGAAGGACC AGCAGTGTGG AGGGTTCACT
    CTGCTCCACC TGATAGCGAC AGGTGTGTCG TGTTTTCTTG GTGCCGGTCT GCTGTCTTTC
    CTGGTGTATG TTTACTGTCA GCGGTTCCAC AAGCCCTCGC AGGAGTCTGC CATCATCCAT
    CCCACCACTC CCAACCACCT CAACTACAAG GGCAACACCA CACCAAAGAA TGAGAAATAC
    ACACCCATGG AGTTCAAGAC TCTGAATAAG AACAATCTCC TGCCAGACGA GAGGACAAAC
    TACTTCCCTT CACCGCTTCA GCAGACCAAC GTCTACACCA CCACGTACTA CCCCACAGGA
    CTGGGCAAAT ACGACTACCG GCCGGACTCC TCACCCTCGC CCTGCAGAAC CTATAACCAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684379.3
    CDS293..3598
    Translation

    Target ORF information:

    RefSeq Version XM_679287.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679287.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GTTAAAGGCG CCTGGTCTTG CTGGTCATCA TGGTCGCAGT GTTCGGTTCC CTGTGGAGGC
    GGACACTACC AACGAACACG CACATGTACA AGCCCTGCCC CTGCCAATGG AGGGGACATC
    TGCATCGGCT TGCACACAGA GGAGGCTCTT TGCAACACGC ATACTTGTGA TGGTGGCTGG
    ATGGCCTGGT CTGTGTGGTC TGAGTGTGAT GATTCAGGCC TGCAGCTGCG CAGTCGAGTG
    TGTGGAGCTC AATCCATGCC ATGTGCGGGA AACAGCTCCC AGCACAGAGA CTGCAATGAG
    ATCCCAGCCA TTCTTCCGGC ATCCAGCTAT GAGAAGGACC AGCAGTGTGG AGGGTTCACT
    CTGCTCCACC TGATAGCGAC AGGTGTGTCG TGTTTTCTTG GTGCCGGTCT GCTGTCTTTC
    CTGGTGTATG TTTACTGTCA GCGGTTCCAC AAGCCCTCGC AGGAGTCTGC CATCATCCAT
    CCCACCACTC CCAACCACCT CAACTACAAG GGCAACACCA CACCAAAGAA TGAGAAATAC
    ACACCCATGG AGTTCAAGAC TCTGAATAAG AACAATCTCC TGCCAGACGA GAGGACAAAC
    TACTTCCCTT CACCGCTTCA GCAGACCAAC GTCTACACCA CCACGTACTA CCCCACAGGA
    CTGGGCAAAT ACGACTACCG GCCGGACTCC TCACCCTCGC CCTGCAGAAC CTATAACCAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa15438
    Clone ID Related Accession (Same CDS sequence) XM_679287.6 , XM_679287.5
    Accession Version XM_679287.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product semaphorin-5B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679287.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GTTAAAGGCG CCTGGTCTTG CTGGTCATCA TGGTCGCAGT GTTCGGTTCC CTGTGGAGGC
    GGACACTACC AACGAACACG CACATGTACA AGCCCTGCCC CTGCCAATGG AGGGGACATC
    TGCATCGGCT TGCACACAGA GGAGGCTCTT TGCAACACGC ATACTTGTGA TGGTGGCTGG
    ATGGCCTGGT CTGTGTGGTC TGAGTGTGAT GATTCAGGCC TGCAGCTGCG CAGTCGAGTG
    TGTGGAGCTC AATCCATGCC ATGTGCGGGA AACAGCTCCC AGCACAGAGA CTGCAATGAG
    ATCCCAGCCA TTCTTCCGGC ATCCAGCTAT GAGAAGGACC AGCAGTGTGG AGGGTTCACT
    CTGCTCCACC TGATAGCGAC AGGTGTGTCG TGTTTTCTTG GTGCCGGTCT GCTGTCTTTC
    CTGGTGTATG TTTACTGTCA GCGGTTCCAC AAGCCCTCGC AGGAGTCTGC CATCATCCAT
    CCCACCACTC CCAACCACCT CAACTACAAG GGCAACACCA CACCAAAGAA TGAGAAATAC
    ACACCCATGG AGTTCAAGAC TCTGAATAAG AACAATCTCC TGCCAGACGA GAGGACAAAC
    TACTTCCCTT CACCGCTTCA GCAGACCAAC GTCTACACCA CCACGTACTA CCCCACAGGA
    CTGGGCAAAT ACGACTACCG GCCGGACTCC TCACCCTCGC CCTGCAGAAC CTATAACCAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684379.3
    CDS259..3564
    Translation

    Target ORF information:

    RefSeq Version XM_679287.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Ba (sema5ba), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679287.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACCGTGT TGCCCCGGGA CGTGGCTTCC CTGGCTGTGC CCGGCCTTAT CCTCTGTTTC 
    ATCACTCTTC CATTTCCGAC GATTCTATCC CAGAATCCTC CTGTCCCAAC AGAGACCTCC
    CCGTCCCCAG CGCCCCACCA CACCAGACCC GAGTGTACCA GATTAGAGCA CCCTGTCGTC
    TCCATTCAAG CTCTAAGTCC TTTGATCTCC TCTTTCTCGC ATCCTGGTGT AAGGGATTAT
    TCCCAGCTGA CCCTTGACCT AACCAGGAAT GAACTTATAG TCGGAGCAAG AAACTACCTC
    TTCAGACTAA ATCTCAGCAA CATTTCACTA ATTCAGGCAA CGGAATGGGG GCCAGACGAA
    GACACCAGGA GGTCCTGTCA AAGCAAAGGG AAGACGGAGC TGGAGTGTCA AAACTACATC
    CGGGTGCTGC TTGTCAACAA GACAGAGGTG GTCACCTGCG GGACAAATGC CTTCCAGCCT
    CTTTGCATCA CCAGAGAGGC AGGGAACATG AGCAGAGTGT TGGAGAGGGT GAACGGAGTG
    GCCCGCTGTC CCTATGACCC CCGTCATAAC TCCACAGCGG TGGTGACTGA AAGTGGAGAG
    CTGTACGCTG CCACGGTCAT CGATTTCTCT GGACGGGACC CTGTCATCTA CCGCAGCCTT
    GGAGGAATGC CGCCTTTGCG GACCGCCCAG TACAACTCCA AATGGCTTAA TGAGCCTCAC
    TTCATCTCGG CTTATGATGT GGGTCTTTTC ACCTTCTTCT TCTTGAGGGA GAACGCAGTG
    GAGCATGACT GCGGTAAAAC GGTGTACTCA CGGGTGGCGC GGGTGTGTAA AAATGACATT
    GGAGGCCGAT TCTTGCTGGA GGACACGTGG ACCACTTTTA CGAAGGCCCG ACTCAACTGC
    TCGCGGTCAG GAGAAATCCC TTTCTACTAT AATGAGCTAC AAAGCACCTT CTACCTGCCC
    GAACAGGACC TCATCTATGG GATCTTCACC ACCAATGTGA ACAGCATTGC TGCCTCTGCA
    GTTTGCGCGT ACAACCTCAG CGCAATCACA CAGGCTTTCA ACGGGCCCTT CCGCTCCCAG
    GAGAACCCTC GCTCCACGTG GCTGCCCACC CCCAACCCCA TCCCCAACTT CCAGTGTGGG
    ACCATAGATG AAGAAGGGCC TAATGAAAGA CTTACCGAAC GCAGTCTGCA GGACGCCCAG
    CGGCTCTTCC TCATGAACGA TGTGGTTCAG CCAGTTTCTG TGGATCCACT GGTCTGGCAG
    GATGACGTCC GCTTCTCTAA GTTGGTTGTT GACATTGTAC AGGGGCAGGA CTCATTGCAT
    CATGTCATGT ACATAGGCAC AGAGTATGGG ACTATTCTGA AGGCGCTGGC AACCACAAAT
    AAGAGCCTGC AAGGTTGCTA CTTGGAGGAG ATGCAGCTCT TCCCTCCTGG ATTGCAGCAG
    CCAATCCTGA GCCTTCAGAT TCTCCATGGC GACCGGACTC TTTTTGTGGG CCTGAATGAC
    AAAGTGCTCA AGATCCCTCT AGCGAGATGC TCCAGCTACA AAACTGAGTT GATGTGTTTG
    GATGCACGCG ACCCTTACTG TGGTTGGGAC CGAAAGCAGC GGCGTTGCAC CACCATTGAG
    GACAGCTCCA ACATGAGCCA GTGGTTCCAG AATATCACTG CCTGCCCGCT GAGGAACCAA
    ACCACAGATG GTGCGTACGG GCCCTGGGCT CCATGGCAGC CCTGTAGCCA TGATGATGGT
    GAAGGCACCA GCACGTGTCT TTGTCGATCG CGGGTATGCA ATAGTCCACC GCCTCGTTGT
    GGAGGAAAAA ACTGCCAGGG TCCCACCATT GAGGTGTCCA ACTGTTCAAG GAATGGAGGA
    TGGACACCAT GGTCGTCCTG GGGCCAGTGC AGCACAAGCT GTGAGATTGG GTTTGAAGTT
    CGCCAGCGGT CCTGTAACAA TCCTTCACCC AGGCATGGAG GCCGAGTGTG TGTGGGGCAA
    AGCAGAGAAC AAAGATTCTG CAATGAGAAG GTGTCATGCC CTCAGCCTAT CTTCTGGTCA
    TCATGGTCTC CCTGGTCCAA GTGCAGTGCA GATTGTGGTG GAGGTGTGCA CTCTCGCTCC
    AGGAACTGTG AGAATGGAAA TAGCTGTCCA GGGTGTGCGC TGGAGTACCA AGCATGTAAT
    TTGGAGTCCT GTCCAGAGGT GCGCCGAAAC ACCCCGTGGA CCCCTTGGAT GCCAGTGAAT
    ATAACCCAAG GAGGGGCACG GCAGGAGCAA AGAGTCCGCT ACATCTGCCG GGCTCAACTA
    GCTGACCCTC ATGAACTCCA ACTGGGCAAG CGTAAGGTGG AGACACGCTT CTGTCCCAAT
    GACGGGATGG TAACCTGCGA AACAGACTCT CTTGTTGAGG AGCTTCTCAG AATGCCTGGT
    GTCCGACTCT CAGGCACTGG CTGGTCATCA TGGGAGATGT GGTCAGCTTG CACTCAGGAA
    TGTGCCAAAG GCTACCGCAC TCGCAAACGC AGCTGCACCA ACACAGATGG CAAAAACATA
    CCCACTGCCT GTCGCGGATC GCCTGTAGAA TATCAGGACT GCAATCCTCA GCCCTGTCCA
    GTTAAAGGCG CCTGGTCTTG CTGGTCATCA TGGTCGCAGT GTTCGGTTCC CTGTGGAGGC
    GGACACTACC AACGAACACG CACATGTACA AGCCCTGCCC CTGCCAATGG AGGGGACATC
    TGCATCGGCT TGCACACAGA GGAGGCTCTT TGCAACACGC ATACTTGTGA TGGTGGCTGG
    ATGGCCTGGT CTGTGTGGTC TGAGTGTGAT GATTCAGGCC TGCAGCTGCG CAGTCGAGTG
    TGTGGAGCTC AATCCATGCC ATGTGCGGGA AACAGCTCCC AGCACAGAGA CTGCAATGAG
    ATCCCAGCCA TTCTTCCGGC ATCCAGCTAT GAGAAGGACC AGCAGTGTGG AGGGTTCACT
    CTGCTCCACC TGATAGCGAC AGGTGTGTCG TGTTTTCTTG GTGCCGGTCT GCTGTCTTTC
    CTGGTGTATG TTTACTGTCA GCGGTTCCAC AAGCCCTCGC AGGAGTCTGC CATCATCCAT
    CCCACCACTC CCAACCACCT CAACTACAAG GGCAACACCA CACCAAAGAA TGAGAAATAC
    ACACCCATGG AGTTCAAGAC TCTGAATAAG AACAATCTCC TGCCAGACGA GAGGACAAAC
    TACTTCCCTT CACCGCTTCA GCAGACCAAC GTCTACACCA CCACGTACTA CCCCACAGGA
    CTGGGCAAAT ACGACTACCG GCCGGACTCC TCACCCTCGC CCTGCAGAAC CTATAACCAC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.