chtf18 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chtf18
Entrez Gene ID	554366
Full Name	CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Synonyms	zgc:113153
General protein information	Preferred Names CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) Names chromosome transmission fidelity protein 18 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110102.3	NP_001103572.2	chromosome transmission fidelity protein 18 homolog

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chtf18	NP_001099243.1
Pan troglodytes (chimpanzee)	CHTF18	XP_003952800.1
	CHTF18	XP_001087813.1
Bos taurus (cattle)	CHTF18	NP_001179389.1
Mus musculus (house mouse)	Chtf18	NP_663384.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008869	XP_319609.4
Oryza sativa (rice)	Os03g0264800	NP_001049644.2
Canis lupus familiaris (dog)	CHTF18	XP_547205.4
Gallus gallus (chicken)	CHTF18	NP_001264989.1
Danio rerio (zebrafish)	chtf18	NP_001103572.2
Arabidopsis thaliana (thale cress)	CTF18	NP_171966.2
Homo sapiens (human)	CHTF18	NP_071375.1
Drosophila melanogaster (fruit fly)	cutlet	NP_787969.1
Xenopus tropicalis (tropical clawed frog)	chtf18	XP_002935905.2

Gene Ontology
Bibliography

Related articles in PubMed

A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis.
Balci TB, Prykhozhij SV, Teh EM, Da'as SI, McBride E, Liwski R, Chute IC, Leger D, Lewis SM, Berman JN
British journal of haematology167(1)48-61(2014 Oct)

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
Thomas HR, Percival SM, Yoder BK, Parant JM
PloS one9(12)e114632(2014)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chtf18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chtf18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00581	NM_001110102.3 Latest version!	Danio rerio CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chtf18 ( NM_001110102.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001103572.2 Danio rerio CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.

Price & Availability↑

CloneID	ODa00581
Clone ID Related Accession (Same CDS sequence)	NM_001110102.3
Accession Version	NM_001110102.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-06
Organism	Danio rerio(zebrafish)
Product	chromosome transmission fidelity protein 18 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX004779.16 and BX323038.3. On Nov 5, 2010 this sequence version replaced NM_001110102.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01005169.1, GFIL01004000.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGGACGAAT ATGACGAAAT GTACGGGATT GAGGATGATT TTGAACGACA GTTCGCAGAT 
GAGTTGGAAG TCATGGCTGA ATTGGAGAGC AACAATCCAC CAGCTGCAAA AGTGTCTGAA 
TTTCGCAATA AGCCGATCAC ACAGACGTTC GAGGAAGCCC TTGTGTCCGG AGACCAGCCA 
TCAAGGAAAT CAGTAAATAA CCAGAATCCA GAGAGCATCT CTCCGCCGGT ATACGAGGAG 
GAAGAGGAGT CTCTGACCCC TAAACCCAAG AAAAGGAGGC AAGATGTGGC GAAAAAGCTC 
CAGTTTGGCG TTGACCAAGA TGATGACATC ACTCCTCCAT CTTCCCCAGA GGTTTACGAC 
AGGGAAGTGA GAGACGGGCC TAGATTTTCC CTAACCCTGA GTCCAGACAG ACCAGCAGCA 
ACAAAAATAA CGACAAATGT TTTAGATATT AGTGGACTGG GAGCTTTACA GGAGTCACCG 
AAGCAGGTGA CGGCAGCAAA ACGTCATGCT CAGAAACGCC CACCTGCAAT CGGAGATTAC 
ATCACAGTCA CAGACTCTAT GGGAAACAGG GTCTACCTTA ACAAGAAAGA GGATGTGGAG 
AAGACACCAG ACCCGAGAGC CTTCCGTAAC TCGCTGAATG GACTAGGACT GCTAGCTGTT 
CCCATTGAAG TGCTCAAGGA ACAGATTGCA GAGAGACGGC ATCGACAAGT GGTGGAGGAA 
TCTCAAAGAC TGACTGAACT TATGAAGAGT GGAATAGATT CAGATCTTCT CGCAGAAGAG 
CAGACAGACT CGGGTGTGGA TGGGGATAGT GGGGAAGATG AAGCATCTTC ACGACTTTGG 
GTGGATCAGT TCTCACCACA GCACTACACT GATCTGCTGA GTGATGATTT TACTAATCGC 
TGTCTGCTGA AATGGCTGAA GCTCTGGGAC ACTGTGGTCT TCGGGCGAGA GAGGAAAAGC 
CGTCCGGTCC CTGTAGAGGC CAGATCAAAT TTTACAAACG CTCAAAATCA AAACCAGGCC 
CAACGTTTCA AAACCAAGTC TCAAATGACA GAAGAGATAC TAGAGGCTGA GCTAGACCAG 
TACAAAAGAC CGAAATTTAA GGTAGCGCTA TTATCAGGTC CTCCTGGACT GGGGAAAACC 
ACACTAGCAC ACATTATTGC AAAGCATGCT GGTTACAATG TAGTAGAAAT CAATGCTAGT 
GATGACCGTA GTGCAGAGCT CTTTCAGAAG CGCATTGATA CTGCCACACA GATGAAGTCT 
GTTCTGGGAG CCAATGAAAA GCCCAACTGC CTTATTATAG ATGAAATCGA CGGAGCTCCT 
GCTGCTGCCA TTAATATCTT ATTGGCCACC CTGAACCGGA AGGACAGCAA GGATGGAGAG 
GAAACAGGAG TCAATGCCTT GAAAAAGAAA AAGAAAAAGC AATCTGTGCT GCTTAGACCA 
ATCATCTGCA TCTGCAATGA TCTATATGTT CCAGCTCTAA GGCCACTGAG ACAGCAAGCT 
TTCCTGCTGG TCTTTCCTCA GACTCTGCCA TCACGCTTGG CTCAAAGATT AGCAGAGATC 
AGCAGGCGTC AGGGAATGAA AGCAGATACA GGCACTCTGA TGGCCCTGTG TGAGAAAACT 
GACAATGACA TCCGTTCTTG CATCAACACA TTACAGTTTT TACACAGTCG TGGACAGAAG 
CACTTGGACC AGCGCAGTGT CAGCTCCATG TGTGTGGGAT TGAAAGACCA AAACAAGGGA 
CTGTTCTCAG TTTGGCAGGA GATCTTTCAG CTTCCTCGAC TGAAAAGGAA GCGGATTGGT 
GGAGATCCTT TCAGTGGTTA TGATGAGTTG GGACCTAAAG AGGGTGCACA AAGGCTTCAG 
CACATCTTAC ATTTGGTCTC ATCTACTGGA GAACATGAGA AACTCACTCA GGGCCTCTAT 
GACAACTTCC TAAGCATGAA GCTGAAGGAT CCTGGGATGT TGGGTGTTTG CTCTGGTCTT 
GACTGGCTGT GTTTCTCAGA CTTGCTGAAT GAGTGTGTGT TGCATGGACA GAACTATTCG 
CTTATGCGCT ACTTCCCCTT CCTGCCCGCT GCCTTTCACC AGCTGTACGC CGCCACCTCT 
GTCCCGCGCA TCAATTATCC ACACAGCCAC TATGAGGCCT TTACCAAAAC TCAGCACATC 
AGGAACGCCC TGCTCGCCAT GTTGAACGAC ATCCCACCTG CCATACAGAG CCGTGTTTGC 
ATGAGCTCTC TCTGTCTGGA CATCCTCAGC CTTCTGCTGG AGCTCATCTC CCCCAAACTG 
AGACCGGTGA ACCCTCAGCT TTACAGCACC AGGGAGAAGC AGCAGCTCTA TGATCTGATC 
GACACAATGA TCAACTACAA CCTGACCTAC AGACAGGACC GAACGCCAGA GGGACAGTAC 
ACTTATGTGC TGGAGCCTAA TATTGAAGAT GTGGTGAATT ATCCTGGTTT ACCTCCTCGG 
AGACAGTTAA CCTATCAGGT GAAGCAGCTC ATCGCCAGGG AGACGGAACT GGAGAGGATG 
AGGAGAGTAG AGAGAGCACA GCAGAGTCGA AACCCACAGA AAATGGAGAC CGTAAATAAG 
AATCAAGAAG AAAAGAAAAC AGAAGTGAAG AAACAGACAA GCAGGAACCA TCAACAGAGG 
CTGGAGAACA TCGTAAAACA GACTGTGGTG GAGACCAGGC CCGAATTGGA CTTTTTTGGA 
CGAGCCATTG TACCCAAGGA GAAGCCTGTG GTCACTGCTA CAAGTGAAGA TGGCAAAGTG 
AGTGGCGTGC TGAACATTGG TAAAGCTGTG GGAAACAGCG ACGTCTGGTT TCGTTTTAAT 
GAAGGTGTGT CCAATGCAGT GCGCAGAAAC GTTTACATTC GAGAGTTGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103572.2
CDS	119..2992
Translation	MDEYDEMYGIEDDFERQFADELEVMAELESNNPPAAKVSEFRNKPITQTFEEALVSGDQPSRKSVNNQNPESISPPVYEEEEESLTPKPKKRRQDVAKKLQFGVDQDDDITPPSSPEVYDREVRDGPRFSLTLSPDRPAATKITTNVLDISGLGALQESPKQVTAAKRHAQKRPPAIGDYITVTDSMGNRVYLNKKEDVEKTPDPRAFRNSLNGLGLLAVPIEVLKEQIAERRHRQVVEESQRLTELMKSGIDSDLLAEEQTDSGVDGDSGEDEASSRLWVDQFSPQHYTDLLSDDFTNRCLLKWLKLWDTVVFGRERKSRPVPVEARSNFTNAQNQNQAQRFKTKSQMTEEILEAELDQYKRPKFKVALLSGPPGLGKTTLAHIIAKHAGYNVVEINASDDRSAELFQKRIDTATQMKSVLGANEKPNCLIIDEIDGAPAAAINILLATLNRKDSKDGEETGVNALKKKKKKQSVLLRPIICICNDLYVPALRPLRQQAFLLVFPQTLPSRLAQRLAEISRRQGMKADTGTLMALCEKTDNDIRSCINTLQFLHSRGQKHLDQRSVSSMCVGLKDQNKGLFSVWQEIFQLPRLKRKRIGGDPFSGYDELGPKEGAQRLQHILHLVSSTGEHEKLTQGLYDNFLSMKLKDPGMLGVCSGLDWLCFSDLLNECVLHGQNYSLMRYFPFLPAAFHQLYAATSVPRINYPHSHYEAFTKTQHIRNALLAMLNDIPPAIQSRVCMSSLCLDILSLLLELISPKLRPVNPQLYSTREKQQLYDLIDTMINYNLTYRQDRTPEGQYTYVLEPNIEDVVNYPGLPPRRQLTYQVKQLIARETELERMRRVERAQQSRNPQKMETVNKNQEEKKTEVKKQTSRNHQQRLENIVKQTVVETRPELDFFGRAIVPKEKPVVTATSEDGKVSGVLNIGKAVGNSDVWFRFNEGVSNAVRRNVYIRELL

Target ORF information:

RefSeq Version	NM_001110102.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (chtf18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110102.3

ORF Insert Sequence:

ATGGACGAAT ATGACGAAAT GTACGGGATT GAGGATGATT TTGAACGACA GTTCGCAGAT 
GAGTTGGAAG TCATGGCTGA ATTGGAGAGC AACAATCCAC CAGCTGCAAA AGTGTCTGAA 
TTTCGCAATA AGCCGATCAC ACAGACGTTC GAGGAAGCCC TTGTGTCCGG AGACCAGCCA 
TCAAGGAAAT CAGTAAATAA CCAGAATCCA GAGAGCATCT CTCCGCCGGT ATACGAGGAG 
GAAGAGGAGT CTCTGACCCC TAAACCCAAG AAAAGGAGGC AAGATGTGGC GAAAAAGCTC 
CAGTTTGGCG TTGACCAAGA TGATGACATC ACTCCTCCAT CTTCCCCAGA GGTTTACGAC 
AGGGAAGTGA GAGACGGGCC TAGATTTTCC CTAACCCTGA GTCCAGACAG ACCAGCAGCA 
ACAAAAATAA CGACAAATGT TTTAGATATT AGTGGACTGG GAGCTTTACA GGAGTCACCG 
AAGCAGGTGA CGGCAGCAAA ACGTCATGCT CAGAAACGCC CACCTGCAAT CGGAGATTAC 
ATCACAGTCA CAGACTCTAT GGGAAACAGG GTCTACCTTA ACAAGAAAGA GGATGTGGAG 
AAGACACCAG ACCCGAGAGC CTTCCGTAAC TCGCTGAATG GACTAGGACT GCTAGCTGTT 
CCCATTGAAG TGCTCAAGGA ACAGATTGCA GAGAGACGGC ATCGACAAGT GGTGGAGGAA 
TCTCAAAGAC TGACTGAACT TATGAAGAGT GGAATAGATT CAGATCTTCT CGCAGAAGAG 
CAGACAGACT CGGGTGTGGA TGGGGATAGT GGGGAAGATG AAGCATCTTC ACGACTTTGG 
GTGGATCAGT TCTCACCACA GCACTACACT GATCTGCTGA GTGATGATTT TACTAATCGC 
TGTCTGCTGA AATGGCTGAA GCTCTGGGAC ACTGTGGTCT TCGGGCGAGA GAGGAAAAGC 
CGTCCGGTCC CTGTAGAGGC CAGATCAAAT TTTACAAACG CTCAAAATCA AAACCAGGCC 
CAACGTTTCA AAACCAAGTC TCAAATGACA GAAGAGATAC TAGAGGCTGA GCTAGACCAG 
TACAAAAGAC CGAAATTTAA GGTAGCGCTA TTATCAGGTC CTCCTGGACT GGGGAAAACC 
ACACTAGCAC ACATTATTGC AAAGCATGCT GGTTACAATG TAGTAGAAAT CAATGCTAGT 
GATGACCGTA GTGCAGAGCT CTTTCAGAAG CGCATTGATA CTGCCACACA GATGAAGTCT 
GTTCTGGGAG CCAATGAAAA GCCCAACTGC CTTATTATAG ATGAAATCGA CGGAGCTCCT 
GCTGCTGCCA TTAATATCTT ATTGGCCACC CTGAACCGGA AGGACAGCAA GGATGGAGAG 
GAAACAGGAG TCAATGCCTT GAAAAAGAAA AAGAAAAAGC AATCTGTGCT GCTTAGACCA 
ATCATCTGCA TCTGCAATGA TCTATATGTT CCAGCTCTAA GGCCACTGAG ACAGCAAGCT 
TTCCTGCTGG TCTTTCCTCA GACTCTGCCA TCACGCTTGG CTCAAAGATT AGCAGAGATC 
AGCAGGCGTC AGGGAATGAA AGCAGATACA GGCACTCTGA TGGCCCTGTG TGAGAAAACT 
GACAATGACA TCCGTTCTTG CATCAACACA TTACAGTTTT TACACAGTCG TGGACAGAAG 
CACTTGGACC AGCGCAGTGT CAGCTCCATG TGTGTGGGAT TGAAAGACCA AAACAAGGGA 
CTGTTCTCAG TTTGGCAGGA GATCTTTCAG CTTCCTCGAC TGAAAAGGAA GCGGATTGGT 
GGAGATCCTT TCAGTGGTTA TGATGAGTTG GGACCTAAAG AGGGTGCACA AAGGCTTCAG 
CACATCTTAC ATTTGGTCTC ATCTACTGGA GAACATGAGA AACTCACTCA GGGCCTCTAT 
GACAACTTCC TAAGCATGAA GCTGAAGGAT CCTGGGATGT TGGGTGTTTG CTCTGGTCTT 
GACTGGCTGT GTTTCTCAGA CTTGCTGAAT GAGTGTGTGT TGCATGGACA GAACTATTCG 
CTTATGCGCT ACTTCCCCTT CCTGCCCGCT GCCTTTCACC AGCTGTACGC CGCCACCTCT 
GTCCCGCGCA TCAATTATCC ACACAGCCAC TATGAGGCCT TTACCAAAAC TCAGCACATC 
AGGAACGCCC TGCTCGCCAT GTTGAACGAC ATCCCACCTG CCATACAGAG CCGTGTTTGC 
ATGAGCTCTC TCTGTCTGGA CATCCTCAGC CTTCTGCTGG AGCTCATCTC CCCCAAACTG 
AGACCGGTGA ACCCTCAGCT TTACAGCACC AGGGAGAAGC AGCAGCTCTA TGATCTGATC 
GACACAATGA TCAACTACAA CCTGACCTAC AGACAGGACC GAACGCCAGA GGGACAGTAC 
ACTTATGTGC TGGAGCCTAA TATTGAAGAT GTGGTGAATT ATCCTGGTTT ACCTCCTCGG 
AGACAGTTAA CCTATCAGGT GAAGCAGCTC ATCGCCAGGG AGACGGAACT GGAGAGGATG 
AGGAGAGTAG AGAGAGCACA GCAGAGTCGA AACCCACAGA AAATGGAGAC CGTAAATAAG 
AATCAAGAAG AAAAGAAAAC AGAAGTGAAG AAACAGACAA GCAGGAACCA TCAACAGAGG 
CTGGAGAACA TCGTAAAACA GACTGTGGTG GAGACCAGGC CCGAATTGGA CTTTTTTGGA 
CGAGCCATTG TACCCAAGGA GAAGCCTGTG GTCACTGCTA CAAGTGAAGA TGGCAAAGTG 
AGTGGCGTGC TGAACATTGG TAAAGCTGTG GGAAACAGCG ACGTCTGGTT TCGTTTTAAT 
GAAGGTGTGT CCAATGCAGT GCGCAGAAAC GTTTACATTC GAGAGTTGCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A transgenic zebrafish model expressing KIT-D816V recapitulates features of aggressive systemic mastocytosis.
British journal of haematology167(1)48-61(2014 Oct)
Balci TB,Prykhozhij SV,Teh EM,Da'as SI,McBride E,Liwski R,Chute IC,Leger D,Lewis SM,Berman JN

High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis.
PloS one9(12)e114632(2014)
Thomas HR,Percival SM,Yoder BK,Parant JM

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

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chtf18 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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