PTHLHA cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTHLHA
Entrez Gene ID	554221
Full Name	parathyroid hormone-like hormone a
Synonyms	pthlh,pthrp,si:ch211-286m4.5
General protein information	Preferred Names parathyroid hormone-like hormone a Names parathyroid hormone-related protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299932.3	XP_009298207.1	parathyroid hormone-related protein isoform X1
NM_001024627.2	NP_001019798.2	parathyroid hormone-related protein precursor
XM_005168511.4	XP_005168568.1	parathyroid hormone-related protein isoform X1
XM_009299932.2	XP_009298207.1	parathyroid hormone-related protein isoform X1
XM_017355018.1	XP_017210507.1	parathyroid hormone-related protein isoform X1
XM_005168511.3	XP_005168568.1	parathyroid hormone-related protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PTHLH	XP_528764.2
	PTHLH	XP_001104176.1
Mus musculus (house mouse)	Pthlh	NP_032996.2
Rattus norvegicus (Norway rat)	Pthlh	NP_036768.1
Homo sapiens (human)	PTHLH	NP_945317.1
Bos taurus (cattle)	PTHLH	NP_777178.1
Canis lupus familiaris (dog)	PTHLH	NP_001003303.1
Danio rerio (zebrafish)	pthlha	NP_001019798.2
Xenopus tropicalis (tropical clawed frog)	pthlh	XP_004912801.1
Gallus gallus (chicken)	PTHLH	NP_001167577.1

Gene Ontology
Bibliography

Related articles in PubMed

Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway.
Suarez-Bregua P, Torres-Nu?ez E, Saxena A, Guerreiro P, Braasch I, Prober DA, Moran P, Cerda-Reverter JM, Du SJ, Adrio F, Power DM, Canario AV, Postlethwait JH, Bronner ME, Ca?estro C, Rotllant J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)569-583(2017 02)

PTH Reloaded: A New Evolutionary Perspective.
Suarez-Bregua P, Cal L, Ca?estro C, Rotllant J
Frontiers in physiology8776(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Functional Pairing of Class B1 Ligand-GPCR in Cephalochordate Provides Evidence of the Origin of PTH and PACAP/Glucagon Receptor Family.
On JS, Duan C, Chow BK, Lee LT
Molecular biology and evolution32(8)2048-59(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of function of either PTHR1 or PTHrP in zebrafish leads to a localized aortic defect that is Notch dependent.
Title: Loss of function of parathyroid hormone receptor 1 induces Notch-dependent aortic defects during zebrafish vascular development.

In normal cartilage development, sox9 upregulates pthlh, which downregulates runx2, and that the duplicated nature of all three of these genes in zebrafish creates a network of regulation by different co-orthologs in different tissues.
Title: Duplicated zebrafish co-orthologs of parathyroid hormone-related peptide (PTHrP, Pthlh) play different roles in craniofacial skeletogenesis.

The following PTHLHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTHLHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34091	NM_001024627.2 Latest version!	Danio rerio parathyroid hormone-like hormone a (pthlha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa08998	XM_005168511.4 Latest version!	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa08998	XM_017355018.1 Latest version!	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa08998	XM_009299932.3 Latest version!	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa08998	XM_009299932.2	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa08998	XM_005168511.3	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTHLHA ( NM_001024627.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001019798.2 Danio rerio parathyroid hormone-like hormone a (pthlha), mRNA.

Price & Availability↑

CloneID	ODa34091
Clone ID Related Accession (Same CDS sequence)	NM_001024627.2
Accession Version	NM_001024627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	parathyroid hormone-related protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ022615.1. On or before Nov 10, 2011 this sequence version replaced NM_001245029.1, NM_001024627.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ022615.1, BC162885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GGCTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019798.2
CDS	231..827
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGAGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	NM_001024627.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024627.2

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GGCTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTHLHA ( XM_009299932.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298207.1 Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08998
Clone ID Related Accession (Same CDS sequence)	XM_009299932.3 , XM_005168511.4 , XM_009299932.2 , XM_017355018.1 , XM_005168511.3
Accession Version	XM_009299932.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	parathyroid hormone-related protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298207.1
CDS	1317..1913
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGTGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	XM_009299932.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299932.3

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTHLHA ( XM_005168511.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168568.1 Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08998
Clone ID Related Accession (Same CDS sequence)	XM_009299932.3 , XM_005168511.4 , XM_009299932.2 , XM_017355018.1 , XM_005168511.3
Accession Version	XM_005168511.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	parathyroid hormone-related protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168511.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168568.1
CDS	231..827
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGTGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	XM_005168511.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168511.4

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTHLHA ( XM_009299932.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298207.1 Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa08998
Clone ID Related Accession (Same CDS sequence)	XM_009299932.3 , XM_005168511.4 , XM_009299932.2 , XM_017355018.1 , XM_005168511.3
Accession Version	XM_009299932.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	parathyroid hormone-related protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298207.1
CDS	468..1064
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGTGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	XM_009299932.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299932.2

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTHLHA ( XM_017355018.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210507.1 Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa08998
Clone ID Related Accession (Same CDS sequence)	XM_009299932.3 , XM_005168511.4 , XM_009299932.2 , XM_017355018.1 , XM_005168511.3
Accession Version	XM_017355018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	parathyroid hormone-related protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210507.1
CDS	360..956
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGTGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	XM_017355018.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355018.1

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTHLHA ( XM_005168511.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168568.1 Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.

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CloneID	ODa08998
Clone ID Related Accession (Same CDS sequence)	XM_009299932.3 , XM_005168511.4 , XM_009299932.2 , XM_017355018.1 , XM_005168511.3
Accession Version	XM_005168511.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	parathyroid hormone-related protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168511.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168568.1
CDS	208..804
Translation	MRMLCCRRVLQQWVFALFLLCSPVPHHGRPVDALSSRMKRSVTHAQLMHDKGRTLQDFKRRMWLQELLHEVHTAEIREAQQPRGGVSISSGAGGGVGTGVSITLPAGVGVSTGAGTAHPKPAGGTKNLPIGFGLEDEEGTNLPQETHKSQNYKDNMMKGIRRKKKSRTGKRREGEKRKRRARSLQEFSSGVHLQPYSC

Target ORF information:

RefSeq Version	XM_005168511.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio parathyroid hormone-like hormone a (pthlha), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168511.3

ORF Insert Sequence:

ATGAGGATGT TGTGTTGCAG ACGGGTTCTC CAGCAGTGGG TCTTTGCTCT GTTTTTACTG 
TGTTCTCCTG TCCCGCACCA CGGCCGGCCC GTCGATGCCC TCAGCAGCCG AATGAAGCGT 
TCAGTGACCC ACGCTCAGCT GATGCATGAT AAAGGCCGAA CGCTGCAGGA TTTTAAGCGG 
CGCATGTGGC TGCAGGAGCT CCTGCATGAA GTGCACACCG CAGAGATCCG GGAGGCGCAG 
CAGCCCAGGG GCGGAGTCAG CATCAGCAGT GGTGCAGGGG GCGGGGTCGG GACTGGAGTC 
TCCATCACAC TGCCTGCAGG AGTAGGCGTG TCCACTGGAG CAGGTACCGC CCACCCCAAA 
CCCGCCGGCG GCACCAAAAA CCTGCCTATA GGCTTTGGGC TGGAAGATGA GGAGGGCACA 
AACCTCCCGC AGGAGACCCA CAAATCCCAG AACTACAAGG ACAACATGAT GAAGGGCATC 
CGGAGGAAGA AGAAGAGCCG CACGGGGAAG AGGAGAGAAG GAGAAAAGAG GAAGAGAAGA 
GCTCGATCAC TGCAGGAGTT CAGCAGCGGC GTCCATCTGC AGCCGTACAG CTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)569-583(2017 02)
Suarez-Bregua P,Torres-Nu?ez E,Saxena A,Guerreiro P,Braasch I,Prober DA,Moran P,Cerda-Reverter JM,Du SJ,Adrio F,Power DM,Canario AV,Postlethwait JH,Bronner ME,Ca?estro C,Rotllant J

PTH Reloaded: A New Evolutionary Perspective.
Frontiers in physiology8776(2017)
Suarez-Bregua P,Cal L,Ca?estro C,Rotllant J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Functional Pairing of Class B1 Ligand-GPCR in Cephalochordate Provides Evidence of the Origin of PTH and PACAP/Glucagon Receptor Family.
Molecular biology and evolution32(8)2048-59(2015 Aug)
On JS,Duan C,Chow BK,Lee LT

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PTHLHA cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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