polb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	polb
Entrez Gene ID	554204
Full Name	polymerase (DNA directed), beta
Synonyms	wu:fa97a05,zgc:109924
General protein information	Preferred Names polymerase (DNA directed), beta Names DNA polymerase beta
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003879.2	NP_001003879.2	DNA polymerase beta

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polb	NP_035260.1
Rattus norvegicus (Norway rat)	Polb	NP_058837.2
Xenopus tropicalis (tropical clawed frog)	polb	NP_001006894.2
Homo sapiens (human)	POLB	NP_002681.1
	POLB	XP_001097548.1
Canis lupus familiaris (dog)	POLB	XP_532790.2
Bos taurus (cattle)	POLB	NP_001029936.1
Gallus gallus (chicken)	POLB	XP_424407.2
Danio rerio (zebrafish)	polb	NP_001003879.1
Pan troglodytes (chimpanzee)	POLB	XP_001143904.1

Gene Ontology
Bibliography

Related articles in PubMed

Graphene oxide nanosheets induce DNA damage and activate the base excision repair (BER) signaling pathway both in?vitro and in?vivo.
Lu CJ, Jiang XF, Junaid M, Ma YB, Jia PP, Wang HB, Pei DS
Chemosphere184795-805(2017 Oct)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Hypoxic Signaling and the Cellular Redox Tumor Environment Determine Sensitivity to MTH1 Inhibition.
Br?utigam L, Pudelko L, Jemth AS, Gad H, Narwal M, Gustafsson R, Karsten S, Carreras Puigvert J, Homan E, Berndt C, Berglund UW, Stenmark P, Helleday T
Cancer research76(8)2366-75(2016 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DNA polymerase beta is sensitive to Mn2+ and Mg2+ and its optimumal pH is 9.
Title: Characterization of DNA polymerase ? from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid.

In brief, the major differences in BER performed by early stage embryos and adults are the absence of DNA polymerase-beta, leading to predominance of replicative polymerases, in early stage embryos.
Title: Base excision repair in early zebrafish development: evidence for DNA polymerase switching and standby AP endonuclease activity.

The following polb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98471	NM_001003879.2 Latest version!	Danio rerio polymerase (DNA directed), beta (polb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polb ( NM_001003879.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003879.2 Danio rerio polymerase (DNA directed), beta (polb), mRNA.

Price & Availability↑

CloneID	ODa98471
Clone ID Related Accession (Same CDS sequence)	NM_001003879.2
Accession Version	NM_001003879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Danio rerio(zebrafish)
Product	DNA polymerase beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954172.9, EH495172.1 and EH521494.1. On Sep 1, 2015 this sequence version replaced NM_001003879.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01030870.1, GDQH01003876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTAAAC GAAAAGCGCC ACAAGAATCC CTGAACGAAG GAATCACTGA TTTTCTCGTT 
GAGCTGGCCA ATTATGAGAG AAATGTCAAC AGAGCCATCC ATAAATACAA TGCTTACAGA 
AAAGCAGCGT CTGTCATCGC GAAATATCCT CAGAAGATCA AAAGTGGGAC AGAAGCAAAG 
AAACTGGATG GAGTCGGTGC AAAGATAGCG GAGAAAATCG ACGAGTTTTT AACAACAGGA 
AAACTGCGCA AGCTGGAAAA GATTCGCAAT GACGACACAA GCTCCTCCAT CAACTTCCTA 
ACCAGAGTGA CTGGAATCGG TCCTGCTGCC GCCAGGAAGT TTTTTGACGA GGGCGTCAGG 
AATTTAGAAG ATTTAAAGAA GATCGAACAC AAACTCAATC ATCATCAGCA GATCGGCTTA 
AAATACTTTG AGGAGTTTGA GAAGAGAATC CCTCGTTCAG AGATGCAAAA AATGGAGGCT 
CTGATATTGA AAGAACTGGA TATAGTGGAT CCAGAGTACA TCGGCACCAT CTGCGGCAGC 
TACAGAAGAG GAGCAGAGTC GAGTGGTGAT ATTGATATAC TGCTGACCCA TCCGGACTTC 
ACATCTCAGT CTGAGAAACA GCCGAAGCTC CTTCATGCCG TTGTGGATCA TCTGGAGTCC 
ATCGGCTTCA TTACTGACAC ACTGTCTAAA GGAGACACTA AATTCATGGG TGTGTGTCAG 
CTGCAGAAAG AAAAAGAAGA AGAGGAGGAG GAGGAGGAGT CTTTACACCG CCGTATTGAT 
ATCAGGCTTA TCCCTAAAGA TCAGTATTAC TGCGGTGTGC TGTATTTCAC CGGCAGTGAT 
ATATTCAACA AGAACATGAG GACTCACGCA CTAGAGAAAG GCTTCACGCT CAATGAGTAC 
ACCATCCGTC CACTGGGCGT CACTGGTGTG GCAGGAGAGC CTCTGCTGGT GGACAGTGAG 
AAGGACATCT TTGAGTACAT CCAGTGGAAA TACAGAGAGC CGAAGGACCG CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003879.2
CDS	138..1157
Translation	MSKRKAPQESLNEGITDFLVELANYERNVNRAIHKYNAYRKAASVIAKYPQKIKSGTEAKKLDGVGAKIAEKIDEFLTTGKLRKLEKIRNDDTSSSINFLTRVTGIGPAAARKFFDEGVRNLEDLKKIEHKLNHHQQIGLKYFEEFEKRIPRSEMQKMEALILKELDIVDPEYIGTICGSYRRGAESSGDIDILLTHPDFTSQSEKQPKLLHAVVDHLESIGFITDTLSKGDTKFMGVCQLQKEKEEEEEEEESLHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRTHALEKGFTLNEYTIRPLGVTGVAGEPLLVDSEKDIFEYIQWKYREPKDRSE

Target ORF information:

RefSeq Version	NM_001003879.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed), beta (polb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003879.2

ORF Insert Sequence:

ATGAGTAAAC GAAAAGCGCC ACAAGAATCC CTGAACGAAG GAATCACTGA TTTTCTCGTT 
GAGCTGGCCA ATTATGAGAG AAATGTCAAC AGAGCCATCC ATAAATACAA TGCTTACAGA 
AAAGCAGCGT CTGTCATCGC GAAATATCCT CAGAAGATCA AAAGTGGGAC AGAAGCAAAG 
AAACTGGATG GAGTCGGTGC AAAGATAGCG GAGAAAATCG ACGAGTTTTT AACAACAGGA 
AAACTGCGCA AGCTGGAAAA GATTCGCAAT GACGACACAA GCTCCTCCAT CAACTTCCTA 
ACCAGAGTGA CTGGAATCGG TCCTGCTGCC GCCAGGAAGT TTTTTGACGA GGGCGTCAGG 
AATTTAGAAG ATTTAAAGAA GATCGAACAC AAACTCAATC ATCATCAGCA GATCGGCTTA 
AAATACTTTG AGGAGTTTGA GAAGAGAATC CCTCGTTCAG AGATGCAAAA AATGGAGGCT 
CTGATATTGA AAGAACTGGA TATAGTGGAT CCAGAGTACA TCGGCACCAT CTGCGGCAGC 
TACAGAAGAG GAGCAGAGTC GAGTGGTGAT ATTGATATAC TGCTGACCCA TCCGGACTTC 
ACATCTCAGT CTGAGAAACA GCCGAAGCTC CTTCATGCCG TTGTGGATCA TCTGGAGTCC 
ATCGGCTTCA TTACTGACAC ACTGTCTAAA GGAGACACTA AATTCATGGG TGTGTGTCAG 
CTGCAGAAAG AAAAAGAAGA AGAGGAGGAG GAGGAGGAGT CTTTACACCG CCGTATTGAT 
ATCAGGCTTA TCCCTAAAGA TCAGTATTAC TGCGGTGTGC TGTATTTCAC CGGCAGTGAT 
ATATTCAACA AGAACATGAG GACTCACGCA CTAGAGAAAG GCTTCACGCT CAATGAGTAC 
ACCATCCGTC CACTGGGCGT CACTGGTGTG GCAGGAGAGC CTCTGCTGGT GGACAGTGAG 
AAGGACATCT TTGAGTACAT CCAGTGGAAA TACAGAGAGC CGAAGGACCG CAGCGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Graphene oxide nanosheets induce DNA damage and activate the base excision repair (BER) signaling pathway both in?vitro and in?vivo.
Chemosphere184795-805(2017 Oct)
Lu CJ,Jiang XF,Junaid M,Ma YB,Jia PP,Wang HB,Pei DS

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Hypoxic Signaling and the Cellular Redox Tumor Environment Determine Sensitivity to MTH1 Inhibition.
Cancer research76(8)2366-75(2016 04)
Br?utigam L,Pudelko L,Jemth AS,Gad H,Narwal M,Gustafsson R,Karsten S,Carreras Puigvert J,Homan E,Berndt C,Berglund UW,Stenmark P,Helleday T

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polb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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