npm1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npm1b
Entrez Gene ID	553507
Full Name	nucleophosmin 1b
Synonyms	npm4
General protein information	Preferred Names nucleophosmin 1b Names nucleophosmin nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) nucleophosmin/nucleoplasmin, 1b nucleophosmin/nucleoplasmin, 4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166155.1	NP_001159627.1	nucleophosmin
XM_005173088.3	XP_005173145.1	nucleophosmin isoform X1
XM_005173088.4	XP_005173145.2	nucleophosmin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus.
Ando K, Parsons MJ, Shah RB, Charendoff CI, Paris SL, Liu PH, Fassio SR, Rohrman BA, Thompson R, Oberst A, Sidi S, Bouchier-Hayes L
The Journal of cell biology216(6)1795-1810(2017 Jun)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The African coelacanth genome provides insights into tetrapod evolution.
Amemiya CT, Alf?ldi J, Lee AP, Fan S, Philippe H, Maccallum I, Braasch I, Manousaki T, Schneider I, Rohner N, Organ C, Chalopin D, Smith JJ, Robinson M, Dorrington RA, Gerdol M, Aken B, Biscotti MA, Barucca M, Baurain D, Berlin AM, Blatch GL, Buonocore F, Burmester T, Campbell MS, Canapa A, Cannon JP, Christoffels A, De Moro G, Edkins AL, Fan L, Fausto AM, Feiner N, Forconi M, Gamieldien J, Gnerre S, Gnirke A, Goldstone JV, Haerty W, Hahn ME, Hesse U, Hoffmann S, Johnson J, Karchner SI, Kuraku S, Lara M, Levin JZ, Litman GW, Mauceli E, Miyake T, Mueller MG, Nelson DR, Nitsche A, Olmo E, Ota T, Pallavicini A, Panji S, Picone B, Ponting CP, Prohaska SJ, Przybylski D, Saha NR, Ravi V, Ribeiro FJ, Sauka-Spengler T, Scapigliati G, Searle SM, Sharpe T, Simakov O, Stadler PF, Stegeman JJ, Sumiyama K, Tabbaa D, Tafer H, Turner-Maier J, van Heusden P, White S, Williams L, Yandell M, Brinkmann H, Volff JN, Tabin CJ, Shubin N, Schartl M, Jaffe DB, Postlethwait JH, Venkatesh B, Di Palma F, Lander ES, Meyer A, Lindblad-Toh K
Nature496(7445)311-6(2013 Apr)

High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design.
Ma AC, Lee HB, Clark KJ, Ekker SC
PloS one8(5)e65259(2013)

Expression of the cytoplasmic NPM1 mutant (NPMc+) causes the expansion of hematopoietic cells in zebrafish.
Bolli N, Payne EM, Grabher C, Lee JS, Johnston AB, Falini B, Kanki JP, Look AT
Blood115(16)3329-40(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following npm1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npm1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03262	NM_001166155.1 Latest version!	Danio rerio nucleophosmin 1b (npm1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
ODa148741	XM_005173088.4 Latest version!	Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
ODa19321	XM_005173088.3 Latest version!	Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npm1b ( NM_001166155.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001159627.1 Danio rerio nucleophosmin 1b (npm1b), mRNA.

Price & Availability↑

CloneID	ODa03262
Clone ID Related Accession (Same CDS sequence)	NM_001166155.1
Accession Version	NM_001166155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nucleophosmin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093285.1. ##Evidence-Data-START## Transcript exon combination :: BC093285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGATA ACATTAAAGA CCTCAGCCGA CCCCAGATGT ATTTGTTTGG TTGTTCGTTG 
AAGGGTGACA AGAAAGAGCA CAAAGTTGAC CTGGATGATG ATGAGGCTGA ACATCAGCTG 
TCACTTAAGT CGGTGTGTTT GGGTGCCGAG GCGGAGGATA AATTCCACAC CGTGGAGACG 
GAGGGATTGA CTTACGATGG CAAAACCACC AAAATCACAC TTGCTGTATT GAAACCATCC 
GTTCTGCCTT CTTTAAGTCT GGGCGGTTTT GAGGTCACTC CTCCTGTGTC CTTTCGTCTG 
CAGTCCGGAG GGGGGCCGGT CTACATCAGC GGTCAACATT TTGTCAGTGT CAAGGAGTCA 
GATGATGAGG ATGAGGAGGA AGAGGAGAAC AACACTTCAC CGGTGAAGAG ACCGTCCAAC 
ATGACCCTCG CCAAAGTGCC TCAGAAAAAG CTGAAAATGG ACTCAGATGA GGATGAAGAC 
AGCGATGATG ATGACGACGA CGATGATGAT GATGATGATG ATGAAGAGGA TGATGACAAA 
CAAGAAAAGC CTGCTGTAAA GAGCCCTGTC AAATCCACTC AAAAAACTCC AGAGAAGAAG 
AAAAGTGCGG ATAAGCAGAA TGGCTCTCCA GACAAGAAAG CAGGGACATC AGGGAAGCCC 
CAAACTCAGA CACCGCAGAA AGTAAAAGAC AAGTCTGCTG CAGGGCCTTC TGGGAAGACT 
CCCAGCATTC CCAGCCTGAG TGAAGTCAAA AGCAAACTTA CATCAGCAGC TAAAGAGGGG 
AAGCCATTTC CAAAGACGGA ACAGAAGTTC GAGAACTTCG CTAGAAGCTC TTTCAAGATC 
TCTGATAAGC AAGTGATCAA AGACCTTTGG AACTTTGTAC AATCACTAAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159627.1
CDS	29..925
Translation	MEDNIKDLSRPQMYLFGCSLKGDKKEHKVDLDDDEAEHQLSLKSVCLGAEAEDKFHTVETEGLTYDGKTTKITLAVLKPSVLPSLSLGGFEVTPPVSFRLQSGGGPVYISGQHFVSVKESDDEDEEEEENNTSPVKRPSNMTLAKVPQKKLKMDSDEDEDSDDDDDDDDDDDDDEEDDDKQEKPAVKSPVKSTQKTPEKKKSADKQNGSPDKKAGTSGKPQTQTPQKVKDKSAAGPSGKTPSIPSLSEVKSKLTSAAKEGKPFPKTEQKFENFARSSFKISDKQVIKDLWNFVQSLKK

Target ORF information:

RefSeq Version	NM_001166155.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleophosmin 1b (npm1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166155.1

ORF Insert Sequence:

ATGGAGGATA ACATTAAAGA CCTCAGCCGA CCCCAGATGT ATTTGTTTGG TTGTTCGTTG 
AAGGGTGACA AGAAAGAGCA CAAAGTTGAC CTGGATGATG ATGAGGCTGA ACATCAGCTG 
TCACTTAAGT CGGTGTGTTT GGGTGCCGAG GCGGAGGATA AATTCCACAC CGTGGAGACG 
GAGGGATTGA CTTACGATGG CAAAACCACC AAAATCACAC TTGCTGTATT GAAACCATCC 
GTTCTGCCTT CTTTAAGTCT GGGCGGTTTT GAGGTCACTC CTCCTGTGTC CTTTCGTCTG 
CAGTCCGGAG GGGGGCCGGT CTACATCAGC GGTCAACATT TTGTCAGTGT CAAGGAGTCA 
GATGATGAGG ATGAGGAGGA AGAGGAGAAC AACACTTCAC CGGTGAAGAG ACCGTCCAAC 
ATGACCCTCG CCAAAGTGCC TCAGAAAAAG CTGAAAATGG ACTCAGATGA GGATGAAGAC 
AGCGATGATG ATGACGACGA CGATGATGAT GATGATGATG ATGAAGAGGA TGATGACAAA 
CAAGAAAAGC CTGCTGTAAA GAGCCCTGTC AAATCCACTC AAAAAACTCC AGAGAAGAAG 
AAAAGTGCGG ATAAGCAGAA TGGCTCTCCA GACAAGAAAG CAGGGACATC AGGGAAGCCC 
CAAACTCAGA CACCGCAGAA AGTAAAAGAC AAGTCTGCTG CAGGGCCTTC TGGGAAGACT 
CCCAGCATTC CCAGCCTGAG TGAAGTCAAA AGCAAACTTA CATCAGCAGC TAAAGAGGGG 
AAGCCATTTC CAAAGACGGA ACAGAAGTTC GAGAACTTCG CTAGAAGCTC TTTCAAGATC 
TCTGATAAGC AAGTGATCAA AGACCTTTGG AACTTTGTAC AATCACTAAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npm1b ( XM_005173088.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173145.2 Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148741
Clone ID Related Accession (Same CDS sequence)	XM_005173088.4
Accession Version	XM_005173088.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleophosmin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATCA ACACGCCGGA ACTACGTTTA ACAGGACCTG TGCGCGCAAA AATGGAGGAT 
AACATTAAAG ACCTCAGCCG ACCCCAGATG TATTTGTTTG GTTGTTCGTT AAAGGCTGAC 
AAGAAAGAGC ACAAAGTTGA CCTGGATGAT GATGAGGCTG AACATCAGCT GTCACTTAAG 
TCGGTGTGTT TGGGTGCCGA GGCGGAGGAT AAATTCCACA CCGTGGAGAT GGAGGGATTG 
ACTTACGATG GCAAAACCAC CAAAATCACA CTTGCTGTAT TGAAACCATC CGTTCTGCCT 
TCTTTAAGTC TGGGCGGTTT TGAGGTCACT CCTCCTGTGT CCTTTCGTCT GCAGTCCGGA 
GGGGGGCCGG TCTACATCAG CGGTCAACAT TTTGTCAGTG TCAAGGAGTC AGATGATGAG 
GATGAGGAGG AAGAGGAGAA CAACACTTCA CCGGTGAAGA GACCGTCCAA CATGACCCTC 
GCCAAAGTGC CTCAGAAAAA GCTGAAAATG GACTCAGATG AGGATGAAGA CAGCGATGAT 
GATGACGACG ACGACGACGA TGATGATGAT GATGAAGAAG AGGATGATGA CAAACAAGAA 
AAGCCTGCTG TAAAGAGCCC TGTCAAATCC ACTCAAAAAA CTCCAGAGAA GAAGAAAAGT 
GCGGATAAGC AGAATGGCTC TCCAGACAAG AAAGCAGGGA CATCAGGGAA GCCCCAAACT 
CAGACACCGC AGAAAGTAAA AGACAAGTCT GCTGCAGGGC CTTCTGGGAA GACTCCCAGC 
ATTCCCAGCC TGAGTGAAGT CAAAAGCAAA CTTACATCAG CAGCTAAAGA GGGGAAGCCA 
TTTCCAAAGA CGGAACAGAA GTTCGAGAAC TTCGCTAGAA GCTCTTTCAA GATCTCTGAT 
AAGCAAGTGA TCAAAGACCT TTGGAACTTT GTACAATCAC TAAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173145.2
CDS	6..956
Translation	MSINTPELRLTGPVRAKMEDNIKDLSRPQMYLFGCSLKADKKEHKVDLDDDEAEHQLSLKSVCLGAEAEDKFHTVEMEGLTYDGKTTKITLAVLKPSVLPSLSLGGFEVTPPVSFRLQSGGGPVYISGQHFVSVKESDDEDEEEEENNTSPVKRPSNMTLAKVPQKKLKMDSDEDEDSDDDDDDDDDDDDDEEEDDDKQEKPAVKSPVKSTQKTPEKKKSADKQNGSPDKKAGTSGKPQTQTPQKVKDKSAAGPSGKTPSIPSLSEVKSKLTSAAKEGKPFPKTEQKFENFARSSFKISDKQVIKDLWNFVQSLKK

Target ORF information:

RefSeq Version	XM_005173088.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173088.4

ORF Insert Sequence:

ATGTCAATCA ACACGCCGGA ACTACGTTTA ACAGGACCTG TGCGCGCAAA AATGGAGGAT 
AACATTAAAG ACCTCAGCCG ACCCCAGATG TATTTGTTTG GTTGTTCGTT AAAGGCTGAC 
AAGAAAGAGC ACAAAGTTGA CCTGGATGAT GATGAGGCTG AACATCAGCT GTCACTTAAG 
TCGGTGTGTT TGGGTGCCGA GGCGGAGGAT AAATTCCACA CCGTGGAGAT GGAGGGATTG 
ACTTACGATG GCAAAACCAC CAAAATCACA CTTGCTGTAT TGAAACCATC CGTTCTGCCT 
TCTTTAAGTC TGGGCGGTTT TGAGGTCACT CCTCCTGTGT CCTTTCGTCT GCAGTCCGGA 
GGGGGGCCGG TCTACATCAG CGGTCAACAT TTTGTCAGTG TCAAGGAGTC AGATGATGAG 
GATGAGGAGG AAGAGGAGAA CAACACTTCA CCGGTGAAGA GACCGTCCAA CATGACCCTC 
GCCAAAGTGC CTCAGAAAAA GCTGAAAATG GACTCAGATG AGGATGAAGA CAGCGATGAT 
GATGACGACG ACGACGACGA TGATGATGAT GATGAAGAAG AGGATGATGA CAAACAAGAA 
AAGCCTGCTG TAAAGAGCCC TGTCAAATCC ACTCAAAAAA CTCCAGAGAA GAAGAAAAGT 
GCGGATAAGC AGAATGGCTC TCCAGACAAG AAAGCAGGGA CATCAGGGAA GCCCCAAACT 
CAGACACCGC AGAAAGTAAA AGACAAGTCT GCTGCAGGGC CTTCTGGGAA GACTCCCAGC 
ATTCCCAGCC TGAGTGAAGT CAAAAGCAAA CTTACATCAG CAGCTAAAGA GGGGAAGCCA 
TTTCCAAAGA CGGAACAGAA GTTCGAGAAC TTCGCTAGAA GCTCTTTCAA GATCTCTGAT 
AAGCAAGTGA TCAAAGACCT TTGGAACTTT GTACAATCAC TAAAAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npm1b ( XM_005173088.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173145.1 Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19321
Clone ID Related Accession (Same CDS sequence)	XM_005173088.3
Accession Version	XM_005173088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleophosmin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGATA ACATTAAAGA CCTCAGCCGA CCCCAGATGT ATTTGTTTGG TTGTTCGTTA 
AAGGCTGACA AGAAAGAGCA CAAAGTTGAC CTGGATGATG ATGAGGCTGA ACATCAGCTG 
TCACTTAAGT CGGTGTGTTT GGGTGCCGAG GCGGAGGATA AATTCCACAC CGTGGAGATG 
GAGGGATTGA CTTACGATGG CAAAACCACC AAAATCACAC TTGCTGTATT GAAACCATCC 
GTTCTGCCTT CTTTAAGTCT GGGCGGTTTT GAGGTCACTC CTCCTGTGTC CTTTCGTCTG 
CAGTCCGGAG GGGGGCCGGT CTACATCAGC GGTCAACATT TTGTCAGTGT CAAGGAGTCA 
GATGATGAGG ATGAGGAGGA AGAGGAGAAC AACACTTCAC CGGTGAAGAG ACCGTCCAAC 
ATGACCCTCG CCAAAGTGCC TCAGAAAAAG CTGAAAATGG ACTCAGATGA GGATGAAGAC 
AGCGATGATG ATGACGACGA CGACGACGAT GATGATGATG ATGAAGAAGA GGATGATGAC 
AAACAAGAAA AGCCTGCTGT AAAGAGCCCT GTCAAATCCA CTCAAAAAAC TCCAGAGAAG 
AAGAAAAGTG CGGATAAGCA GAATGGCTCT CCAGACAAGA AAGCAGGGAC ATCAGGGAAG 
CCCCAAACTC AGACACCGCA GAAAGTAAAA GACAAGTCTG CTGCAGGGCC TTCTGGGAAG 
ACTCCCAGCA TTCCCAGCCT GAGTGAAGTC AAAAGCAAAC TTACATCAGC AGCTAAAGAG 
GGGAAGCCAT TTCCAAAGAC GGAACAGAAG TTCGAGAACT TCGCTAGAAG CTCTTTCAAG 
ATCTCTGATA AGCAAGTGAT CAAAGACCTT TGGAACTTTG TACAATCACT AAAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173145.1
CDS	44..943
Translation	MEDNIKDLSRPQMYLFGCSLKADKKEHKVDLDDDEAEHQLSLKSVCLGAEAEDKFHTVEMEGLTYDGKTTKITLAVLKPSVLPSLSLGGFEVTPPVSFRLQSGGGPVYISGQHFVSVKESDDEDEEEEENNTSPVKRPSNMTLAKVPQKKLKMDSDEDEDSDDDDDDDDDDDDDEEEDDDKQEKPAVKSPVKSTQKTPEKKKSADKQNGSPDKKAGTSGKPQTQTPQKVKDKSAAGPSGKTPSIPSLSEVKSKLTSAAKEGKPFPKTEQKFENFARSSFKISDKQVIKDLWNFVQSLKK

Target ORF information:

RefSeq Version	XM_005173088.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleophosmin 1b (nucleolar phosphoprotein B23, numatrin) (npm1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173088.3

ORF Insert Sequence:

ATGGAGGATA ACATTAAAGA CCTCAGCCGA CCCCAGATGT ATTTGTTTGG TTGTTCGTTA 
AAGGCTGACA AGAAAGAGCA CAAAGTTGAC CTGGATGATG ATGAGGCTGA ACATCAGCTG 
TCACTTAAGT CGGTGTGTTT GGGTGCCGAG GCGGAGGATA AATTCCACAC CGTGGAGATG 
GAGGGATTGA CTTACGATGG CAAAACCACC AAAATCACAC TTGCTGTATT GAAACCATCC 
GTTCTGCCTT CTTTAAGTCT GGGCGGTTTT GAGGTCACTC CTCCTGTGTC CTTTCGTCTG 
CAGTCCGGAG GGGGGCCGGT CTACATCAGC GGTCAACATT TTGTCAGTGT CAAGGAGTCA 
GATGATGAGG ATGAGGAGGA AGAGGAGAAC AACACTTCAC CGGTGAAGAG ACCGTCCAAC 
ATGACCCTCG CCAAAGTGCC TCAGAAAAAG CTGAAAATGG ACTCAGATGA GGATGAAGAC 
AGCGATGATG ATGACGACGA CGACGACGAT GATGATGATG ATGAAGAAGA GGATGATGAC 
AAACAAGAAA AGCCTGCTGT AAAGAGCCCT GTCAAATCCA CTCAAAAAAC TCCAGAGAAG 
AAGAAAAGTG CGGATAAGCA GAATGGCTCT CCAGACAAGA AAGCAGGGAC ATCAGGGAAG 
CCCCAAACTC AGACACCGCA GAAAGTAAAA GACAAGTCTG CTGCAGGGCC TTCTGGGAAG 
ACTCCCAGCA TTCCCAGCCT GAGTGAAGTC AAAAGCAAAC TTACATCAGC AGCTAAAGAG 
GGGAAGCCAT TTCCAAAGAC GGAACAGAAG TTCGAGAACT TCGCTAGAAG CTCTTTCAAG 
ATCTCTGATA AGCAAGTGAT CAAAGACCTT TGGAACTTTG TACAATCACT AAAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus.
The Journal of cell biology216(6)1795-1810(2017 Jun)
Ando K,Parsons MJ,Shah RB,Charendoff CI,Paris SL,Liu PH,Fassio SR,Rohrman BA,Thompson R,Oberst A,Sidi S,Bouchier-Hayes L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The African coelacanth genome provides insights into tetrapod evolution.
Nature496(7445)311-6(2013 Apr)
Amemiya CT,Alf?ldi J,Lee AP,Fan S,Philippe H,Maccallum I,Braasch I,Manousaki T,Schneider I,Rohner N,Organ C,Chalopin D,Smith JJ,Robinson M,Dorrington RA,Gerdol M,Aken B,Biscotti MA,Barucca M,Baurain D,Berlin AM,Blatch GL,Buonocore F,Burmester T,Campbell MS,Canapa A,Cannon JP,Christoffels A,De Moro G,Edkins AL,Fan L,Fausto AM,Feiner N,Forconi M,Gamieldien J,Gnerre S,Gnirke A,Goldstone JV,Haerty W,Hahn ME,Hesse U,Hoffmann S,Johnson J,Karchner SI,Kuraku S,Lara M,Levin JZ,Litman GW,Mauceli E,Miyake T,Mueller MG,Nelson DR,Nitsche A,Olmo E,Ota T,Pallavicini A,Panji S,Picone B,Ponting CP,Prohaska SJ,Przybylski D,Saha NR,Ravi V,Ribeiro FJ,Sauka-Spengler T,Scapigliati G,Searle SM,Sharpe T,Simakov O,Stadler PF,Stegeman JJ,Sumiyama K,Tabbaa D,Tafer H,Turner-Maier J,van Heusden P,White S,Williams L,Yandell M,Brinkmann H,Volff JN,Tabin CJ,Shubin N,Schartl M,Jaffe DB,Postlethwait JH,Venkatesh B,Di Palma F,Lander ES,Meyer A,Lindblad-Toh K

High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design.
PloS one8(5)e65259(2013)
Ma AC,Lee HB,Clark KJ,Ekker SC

Expression of the cytoplasmic NPM1 mutant (NPMc+) causes the expansion of hematopoietic cells in zebrafish.
Blood115(16)3329-40(2010 Apr)
Bolli N,Payne EM,Grabher C,Lee JS,Johnston AB,Falini B,Kanki JP,Look AT

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npm1b cDNA ORF clone, Danio rerio(zebrafish)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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