zgc:162472 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgc:162472
Entrez Gene ID	553495
Full Name	zgc:162472
Synonyms	im:7160152,wu:fc57d05
General protein information	Preferred Names zgc:162472 Names uncharacterized protein LOC553495
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005161021.3	XP_005161078.1	uncharacterized protein LOC553495 isoform X1
XM_017352778.1	XP_017208267.1	uncharacterized protein LOC553495 isoform X1
XM_009295208.2	XP_009293483.1	uncharacterized protein LOC553495 isoform X4
XM_009295207.2	XP_009293482.1	uncharacterized protein LOC553495 isoform X3
XM_017352777.1	XP_017208266.1	uncharacterized protein LOC553495 isoform X1
XM_009295206.2	XP_009293481.1	uncharacterized protein LOC553495 isoform X2
XM_009295207.3	XP_009293482.1	uncharacterized protein LOC553495 isoform X3
NM_001083820.1	NP_001077289.1	uncharacterized protein LOC553495

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zgc:162472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:162472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05012	NM_001083820.1 Latest version!	Danio rerio zgc:162472 (zgc:162472), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa25600	XM_005161021.3 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25600	XM_017352777.1 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25600	XM_017352778.1 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25601	XM_009295206.2 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25602	XM_009295207.3 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41968	XM_009295208.2 Latest version!	Danio rerio zgc:162472 (zgc:162472), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa25602	XM_009295207.2	Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgc:162472 ( NM_001083820.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077289.1 Danio rerio zgc:162472 (zgc:162472), mRNA.

Price & Availability↑

CloneID	ODa05012
Clone ID Related Accession (Same CDS sequence)	NM_001083820.1
Accession Version	NM_001083820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC135001.1. On Apr 5, 2007 this sequence version replaced XM_684790.2. ##Evidence-Data-START## Transcript exon combination :: BC135001.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTCCGG CCAAATGTAA AGCCCACAGG CCGAGCTCTG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGC CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCCACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACCCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTAAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAACAATGCA TGGAGAATAG ATAAAGCTTT CAGAGAAGCG 
ACGTTTGGAT CTAGATTTCT TCAAAAAGTT AATGGAGCAG ATTCTAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077289.1
CDS	103..1713
Translation	MMRRSRISVRPNVKPTGRALTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQASAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQPIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFREATFGSRFLQKVNGADSK

Target ORF information:

RefSeq Version	NM_001083820.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083820.1

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTCCGG CCAAATGTAA AGCCCACAGG CCGAGCTCTG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGC CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCCACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACCCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTAAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAACAATGCA TGGAGAATAG ATAAAGCTTT CAGAGAAGCG 
ACGTTTGGAT CTAGATTTCT TCAAAAAGTT AATGGAGCAG ATTCTAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_005161021.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161078.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25600
Clone ID Related Accession (Same CDS sequence)	XM_005161021.3 , XM_017352778.1 , XM_017352777.1
Accession Version	XM_005161021.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161078.1
CDS	107..4990
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTEQRSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_005161021.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161021.3

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_017352778.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208267.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X3, mRNA.

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CloneID	ODa25600
Clone ID Related Accession (Same CDS sequence)	XM_005161021.3 , XM_017352778.1 , XM_017352777.1
Accession Version	XM_017352778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208267.1
CDS	84..4967
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTEQRSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_017352778.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352778.1

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_017352777.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208266.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X2, mRNA.

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CloneID	ODa25600
Clone ID Related Accession (Same CDS sequence)	XM_005161021.3 , XM_017352778.1 , XM_017352777.1
Accession Version	XM_017352777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208266.1
CDS	98..4981
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTEQRSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_017352777.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352777.1

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCAGCGC 
TCAGAAGAAG AGCCCACCAG TGTCTCCAAG TACTTTTTCA GCGACATCTT TACTGAAGTT 
GATGAATTAG AGGACATGGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_009295206.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293481.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X4, mRNA.

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CloneID	ODa25601
Clone ID Related Accession (Same CDS sequence)	XM_009295206.2
Accession Version	XM_009295206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGATAAC 
CCTGAGGACT GTGAAAATGT TACTTCAGAC ACTCAGTCAC CGGATGACAG GTTAAAGGAC 
TCAGAATCAT CAAAGAACAT AAACAAGAGT CCTTCTATTA AACCTGCTCT GCTGAAGGGT 
CGACCTCAGA AACTGATCCC GAACATCAGC CGCAGATGGG GCGAACGACG GCCCAAAAAC 
AACATCCAGG AAAACAGGAC TTCGGCTAAA GATGAAGATC CTCTCAAGGT GCCTTCCGCC 
TCAAAAGAAG AGAAAGACAA GAAACAGGCT TCAGTTATCA TTGATCTGAT TGATTTAGAT 
GAAGAACCGG ACCTGAGTGC TGTGCAAGAA CAGATATTCA ATAAACCAAC CAGGTCAGGA 
AGGATCCCCA AGCTCTCTCA GCATGTGATA CAGGCAGCGG CAGAGGAGGA GGAAGATGAG 
GAAGAGCCCT CTGATCCCCC TACCTCTTCC AAAGTTTGTG TTCCATCACC TGGCTGTAGG 
GCTAAAATAA AACCAGGCCC CAGACTAAAA CAGGGCATGC AGAGGAGAGG GAAATCTAGA 
CTGGTGACTT TACTGGCCTC TGGGGCTGAA GAAGATGATG ATGAAGAGAG AGAGGAAGCA 
GAGGGTTGCA TCCTGAGCCA GGAGGACTTT CCTTCAAACT CTGAAGAGGA AAACCAGGCA 
TTTGTGCCAA TGAGTCTACG ACCTCAAGAT CATTTTGATT CAGAGGTGGT AGAAACCATG 
GAAGAGCTGG ACATTTCAGC GAATGTGCCT GATATCTTGA GCGCGTCCCA CAATGCTTTG 
TGCCCCGAGT CTGCATGTGA GCAGGCCGTA TTTCCTGCTG GTCCTGTCTC GTATGAACAT 
CAGTTGGACC TTCTCGTTGA TGTCATTGAG TTTCTTGACC CAGAACACAT GGAAGTATGT 
AAAGAGATCA ACAATGAAGC AGCTCAGACT CTTCTGACCA TCGGCAACTC GGCTCAGATG 
ATCCAGACCT CAGAAATGCC CTGCTTAAGT GGACATGATA TTGATGTGCA TGAAGAAATC 
GCTAATGAGG AAGTCTTCAC AGAAACGGCC ATCATGTCTG ATCCTTCTAT AAGCTGTTAT 
TCAGAGATGA GAAGTGATGT TGAACTTTCA AACAGTGAAA CTCAAATCCC AGAACCTCCC 
ACTTCAATAG AAGAGCCAAT CAAATCACAG AATTCAATGT CTCATCCTGG TCCCGTTCAG 
CAAGTCTCAC AGGATCTGGC ATCTACTTTT GTTCCTTCTT CAACAAGAGG CCGTTTCGCC 
AAACCCAAGC CCAATATTGG TCTGAAAAGC AGACGAGCTC CTCAGCAGCA GATCAGTTCA 
GAACTTCCTG CAGATTCTGT GGAGAACTCA AAGAGTCTGA CTTCGGCAGA ACAGGATGAA 
AAACAAAAGG CATCCATGCA AGAGATTGCA GATGAACTGT CAGACTCCTC TAACATACAC 
CCTGAGGTCC AGCAGCCGGG TACAGTATCT GATAGAAGAG AAGATGCTCC TGAAAGACTG 
ACTGGAGGTG ATAGTGGATC TGCTGTGTCA TTAAAGAGAA AAGATGAAGA CGAAATCACA 
GAGGAGAAGA TAAAAGAACA CGAAGAGGCT AAATCAGATC CAGTGCTTAT TAATAGTGAG 
AGTGAGTCTC AGAGCACACT AGACGAGGCT CCTAAACCTG TCAGGAGGAA CAGGGGACCC 
AAACCTAAAC CAAACCTGAC TCAAGCATCC AGAAAAAAAC ACACACAAAC ACAACTCAAC 
ACTCCTTCAC TCACAACAGG AATAACTTTC ACTAAAAGTC CTGAAGAGGG CGCAGTTATT 
ACCACAGAAC CTGATGTTTC TCAAAGAGCA ACACAACAGG AGGTTACATT GAGGCTTGAA 
TCCACACAGG CTGTCAAAGA TATTTCTCCA GTTGTTTCTG AAGACAATTC CAGACCGAAT 
ACTCTGGTCA GATTAGATGA AGAACAAAAC ATAACGGCTC CAGATGAAGC TTTTGAAGAC 
AGCAGTGTGA AACATTCAAG ATGTGAATCT AAAGATGCTT CTGAGGAATC CATGTTTATA 
CTGTCTCTGA CAGAAATCCC ACCCGCTTTA CAACAGGGGG CGTGTCTTAG GACAGAACCC 
CTCCCACCCA CAATTGAGCC CGGCTTGCAT TCTCATGGTC AAAGTGTGAA TGAGAGCAAG 
GAAGTGTCTC ACCTTCTGCT CACAGATGCT TTAGTTCTTG TGTCTGAAGA AGAGGAGAAG 
GAAGGAGAAG AAGAAGACAA ATCCAGGAGA GGACAACTGA AACGCAAGAC ACCAGCCTCG 
ACATCTCAAG AGAGTACCAA AAGTGAGAGT CAAGCAGAAG ATTGTGTCGT GGAAGTTTCA 
GAAAGCCCTG TGGCAGAAGA GACTGATGAA CATCTGGAAA AGAAAAGAAA ACCACCGGAG 
AGAACAAGGA GAGCAAAGCT GCAGGTTAAA CCCATCCCTG TTATGAGAAA AAACACTCGA 
GGCGTTAAAG AGGACACAGC AGCTCTTACC TTAAAAGAAA CTCCTTCATT ACCCATTTCA 
ACAGCAGAGA CAAAACCAGA GCAAACTGAC CCCACCGTCA TTTCAACATC AGCAAGCAGT 
GATAATATTC CCTCCTCTGA AGAGTCTGTC CAGGAAGAAT CTTCTCAAGG CACTGTGGGA 
CAGGTGGACA TCACAGTAAA AGAGACAGAT GAGTTGGCTA GTGGATCAGA CTCACAAACT 
GCTACTCAAA TCACGCCTGT TGCCACAAGT GGGCCACTTA CAAGGCCTGG TAGAAAACCC 
AAAGGCTTCT TATCCTTCAT TTCAACAAAA AGTACACAAG GAGCACCAAC ACGTCATCGA 
GGATCCAAAC CAGGAGTCAA CACTGCCCGT CCTGACAGAA AACAGGTAGC GAGTGCATCA 
GCAAACCTTG GAGTAAAGCG AGCTTTACCA ACTCCTGCTT CCACCACTGA GCAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293481.1
CDS	107..4987
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTEQRSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_009295206.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295206.2

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGATAAC 
CCTGAGGACT GTGAAAATGT TACTTCAGAC ACTCAGTCAC CGGATGACAG GTTAAAGGAC 
TCAGAATCAT CAAAGAACAT AAACAAGAGT CCTTCTATTA AACCTGCTCT GCTGAAGGGT 
CGACCTCAGA AACTGATCCC GAACATCAGC CGCAGATGGG GCGAACGACG GCCCAAAAAC 
AACATCCAGG AAAACAGGAC TTCGGCTAAA GATGAAGATC CTCTCAAGGT GCCTTCCGCC 
TCAAAAGAAG AGAAAGACAA GAAACAGGCT TCAGTTATCA TTGATCTGAT TGATTTAGAT 
GAAGAACCGG ACCTGAGTGC TGTGCAAGAA CAGATATTCA ATAAACCAAC CAGGTCAGGA 
AGGATCCCCA AGCTCTCTCA GCATGTGATA CAGGCAGCGG CAGAGGAGGA GGAAGATGAG 
GAAGAGCCCT CTGATCCCCC TACCTCTTCC AAAGTTTGTG TTCCATCACC TGGCTGTAGG 
GCTAAAATAA AACCAGGCCC CAGACTAAAA CAGGGCATGC AGAGGAGAGG GAAATCTAGA 
CTGGTGACTT TACTGGCCTC TGGGGCTGAA GAAGATGATG ATGAAGAGAG AGAGGAAGCA 
GAGGGTTGCA TCCTGAGCCA GGAGGACTTT CCTTCAAACT CTGAAGAGGA AAACCAGGCA 
TTTGTGCCAA TGAGTCTACG ACCTCAAGAT CATTTTGATT CAGAGGTGGT AGAAACCATG 
GAAGAGCTGG ACATTTCAGC GAATGTGCCT GATATCTTGA GCGCGTCCCA CAATGCTTTG 
TGCCCCGAGT CTGCATGTGA GCAGGCCGTA TTTCCTGCTG GTCCTGTCTC GTATGAACAT 
CAGTTGGACC TTCTCGTTGA TGTCATTGAG TTTCTTGACC CAGAACACAT GGAAGTATGT 
AAAGAGATCA ACAATGAAGC AGCTCAGACT CTTCTGACCA TCGGCAACTC GGCTCAGATG 
ATCCAGACCT CAGAAATGCC CTGCTTAAGT GGACATGATA TTGATGTGCA TGAAGAAATC 
GCTAATGAGG AAGTCTTCAC AGAAACGGCC ATCATGTCTG ATCCTTCTAT AAGCTGTTAT 
TCAGAGATGA GAAGTGATGT TGAACTTTCA AACAGTGAAA CTCAAATCCC AGAACCTCCC 
ACTTCAATAG AAGAGCCAAT CAAATCACAG AATTCAATGT CTCATCCTGG TCCCGTTCAG 
CAAGTCTCAC AGGATCTGGC ATCTACTTTT GTTCCTTCTT CAACAAGAGG CCGTTTCGCC 
AAACCCAAGC CCAATATTGG TCTGAAAAGC AGACGAGCTC CTCAGCAGCA GATCAGTTCA 
GAACTTCCTG CAGATTCTGT GGAGAACTCA AAGAGTCTGA CTTCGGCAGA ACAGGATGAA 
AAACAAAAGG CATCCATGCA AGAGATTGCA GATGAACTGT CAGACTCCTC TAACATACAC 
CCTGAGGTCC AGCAGCCGGG TACAGTATCT GATAGAAGAG AAGATGCTCC TGAAAGACTG 
ACTGGAGGTG ATAGTGGATC TGCTGTGTCA TTAAAGAGAA AAGATGAAGA CGAAATCACA 
GAGGAGAAGA TAAAAGAACA CGAAGAGGCT AAATCAGATC CAGTGCTTAT TAATAGTGAG 
AGTGAGTCTC AGAGCACACT AGACGAGGCT CCTAAACCTG TCAGGAGGAA CAGGGGACCC 
AAACCTAAAC CAAACCTGAC TCAAGCATCC AGAAAAAAAC ACACACAAAC ACAACTCAAC 
ACTCCTTCAC TCACAACAGG AATAACTTTC ACTAAAAGTC CTGAAGAGGG CGCAGTTATT 
ACCACAGAAC CTGATGTTTC TCAAAGAGCA ACACAACAGG AGGTTACATT GAGGCTTGAA 
TCCACACAGG CTGTCAAAGA TATTTCTCCA GTTGTTTCTG AAGACAATTC CAGACCGAAT 
ACTCTGGTCA GATTAGATGA AGAACAAAAC ATAACGGCTC CAGATGAAGC TTTTGAAGAC 
AGCAGTGTGA AACATTCAAG ATGTGAATCT AAAGATGCTT CTGAGGAATC CATGTTTATA 
CTGTCTCTGA CAGAAATCCC ACCCGCTTTA CAACAGGGGG CGTGTCTTAG GACAGAACCC 
CTCCCACCCA CAATTGAGCC CGGCTTGCAT TCTCATGGTC AAAGTGTGAA TGAGAGCAAG 
GAAGTGTCTC ACCTTCTGCT CACAGATGCT TTAGTTCTTG TGTCTGAAGA AGAGGAGAAG 
GAAGGAGAAG AAGAAGACAA ATCCAGGAGA GGACAACTGA AACGCAAGAC ACCAGCCTCG 
ACATCTCAAG AGAGTACCAA AAGTGAGAGT CAAGCAGAAG ATTGTGTCGT GGAAGTTTCA 
GAAAGCCCTG TGGCAGAAGA GACTGATGAA CATCTGGAAA AGAAAAGAAA ACCACCGGAG 
AGAACAAGGA GAGCAAAGCT GCAGGTTAAA CCCATCCCTG TTATGAGAAA AAACACTCGA 
GGCGTTAAAG AGGACACAGC AGCTCTTACC TTAAAAGAAA CTCCTTCATT ACCCATTTCA 
ACAGCAGAGA CAAAACCAGA GCAAACTGAC CCCACCGTCA TTTCAACATC AGCAAGCAGT 
GATAATATTC CCTCCTCTGA AGAGTCTGTC CAGGAAGAAT CTTCTCAAGG CACTGTGGGA 
CAGGTGGACA TCACAGTAAA AGAGACAGAT GAGTTGGCTA GTGGATCAGA CTCACAAACT 
GCTACTCAAA TCACGCCTGT TGCCACAAGT GGGCCACTTA CAAGGCCTGG TAGAAAACCC 
AAAGGCTTCT TATCCTTCAT TTCAACAAAA AGTACACAAG GAGCACCAAC ACGTCATCGA 
GGATCCAAAC CAGGAGTCAA CACTGCCCGT CCTGACAGAA AACAGGTAGC GAGTGCATCA 
GCAAACCTTG GAGTAAAGCG AGCTTTACCA ACTCCTGCTT CCACCACTGA GCAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_009295207.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293482.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa25602
Clone ID Related Accession (Same CDS sequence)	XM_009295207.2 , XM_009295207.3
Accession Version	XM_009295207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC553495 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293482.1
CDS	107..4987
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTERSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_009295207.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295207.2

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_009295207.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293482.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.

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CloneID	ODa25602
Clone ID Related Accession (Same CDS sequence)	XM_009295207.2 , XM_009295207.3
Accession Version	XM_009295207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293482.1
CDS	112..4992
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEELDISANVPDILSASHNALCPESACEQAVFPAGPVSYEHQLDLLVDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTERSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_009295207.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295207.3

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGC TGGACATTTC AGCGAATGTG CCTGATATCT TGAGCGCGTC CCACAATGCT 
TTGTGCCCCG AGTCTGCATG TGAGCAGGCC GTATTTCCTG CTGGTCCTGT CTCGTATGAA 
CATCAGTTGG ACCTTCTCGT TGATGTCATT GAGTTTCTTG ACCCAGAACA CATGGAAGTA 
TGTAAAGAGA TCAACAATGA AGCAGCTCAG ACTCTTCTGA CCATCGGCAA CTCGGCTCAG 
ATGATCCAGA CCTCAGAAAT GCCCTGCTTA AGTGGACATG ATATTGATGT GCATGAAGAA 
ATCGCTAATG AGGAAGTCTT CACAGAAACG GCCATCATGT CTGATCCTTC TATAAGCTGT 
TATTCAGAGA TGAGAAGTGA TGTTGAACTT TCAAACAGTG AAACTCAAAT CCCAGAACCT 
CCCACTTCAA TAGAAGAGCC AATCAAATCA CAGAATTCAA TGTCTCATCC TGGTCCCGTT 
CAGCAAGTCT CACAGGATCT GGCATCTACT TTTGTTCCTT CTTCAACAAG AGGCCGTTTC 
GCCAAACCCA AGCCCAATAT TGGTCTGAAA AGCAGACGAG CTCCTCAGCA GCAGATCAGT 
TCAGAACTTC CTGCAGATTC TGTGGAGAAC TCAAAGAGTC TGACTTCGGC AGAACAGGAT 
GAAAAACAAA AGGCATCCAT GCAAGAGATT GCAGATGAAC TGTCAGACTC CTCTAACATA 
CACCCTGAGG TCCAGCAGCC GGGTACAGTA TCTGATAGAA GAGAAGATGC TCCTGAAAGA 
CTGACTGGAG GTGATAGTGG ATCTGCTGTG TCATTAAAGA GAAAAGATGA AGACGAAATC 
ACAGAGGAGA AGATAAAAGA ACACGAAGAG GCTAAATCAG ATCCAGTGCT TATTAATAGT 
GAGAGTGAGT CTCAGAGCAC ACTAGACGAG GCTCCTAAAC CTGTCAGGAG GAACAGGGGA 
CCCAAACCTA AACCAAACCT GACTCAAGCA TCCAGAAAAA AACACACACA AACACAACTC 
AACACTCCTT CACTCACAAC AGGAATAACT TTCACTAAAA GTCCTGAAGA GGGCGCAGTT 
ATTACCACAG AACCTGATGT TTCTCAAAGA GCAACACAAC AGGAGGTTAC ATTGAGGCTT 
GAATCCACAC AGGCTGTCAA AGATATTTCT CCAGTTGTTT CTGAAGACAA TTCCAGACCG 
AATACTCTGG TCAGATTAGA TGAAGAACAA AACATAACGG CTCCAGATGA AGCTTTTGAA 
GACAGCAGTG TGAAACATTC AAGATGTGAA TCTAAAGATG CTTCTGAGGA ATCCATGTTT 
ATACTGTCTC TGACAGAAAT CCCACCCGCT TTACAACAGG GGGCGTGTCT TAGGACAGAA 
CCCCTCCCAC CCACAATTGA GCCCGGCTTG CATTCTCATG GTCAAAGTGT GAATGAGAGC 
AAGGAAGTGT CTCACCTTCT GCTCACAGAT GCTTTAGTTC TTGTGTCTGA AGAAGAGGAG 
AAGGAAGGAG AAGAAGAAGA CAAATCCAGG AGAGGACAAC TGAAACGCAA GACACCAGCC 
TCGACATCTC AAGAGAGTAC CAAAAGTGAG AGTCAAGCAG AAGATTGTGT CGTGGAAGTT 
TCAGAAAGCC CTGTGGCAGA AGAGACTGAT GAACATCTGG AAAAGAAAAG AAAACCACCG 
GAGAGAACAA GGAGAGCAAA GCTGCAGGTT AAACCCATCC CTGTTATGAG AAAAAACACT 
CGAGGCGTTA AAGAGGACAC AGCAGCTCTT ACCTTAAAAG AAACTCCTTC ATTACCCATT 
TCAACAGCAG AGACAAAACC AGAGCAAACT GACCCCACCG TCATTTCAAC ATCAGCAAGC 
AGTGATAATA TTCCCTCCTC TGAAGAGTCT GTCCAGGAAG AATCTTCTCA AGGCACTGTG 
GGACAGGTGG ACATCACAGT AAAAGAGACA GATGAGTTGG CTAGTGGATC AGACTCACAA 
ACTGCTACTC AAATCACGCC TGTTGCCACA AGTGGGCCAC TTACAAGGCC TGGTAGAAAA 
CCCAAAGGCT TCTTATCCTT CATTTCAACA AAAAGTACAC AAGGAGCACC AACACGTCAT 
CGAGGATCCA AACCAGGAGT CAACACTGCC CGTCCTGACA GAAAACAGGT AGCGAGTGCA 
TCAGCAAACC TTGGAGTAAA GCGAGCTTTA CCAACTCCTG CTTCCACCAC TGAGCGCTCA 
GAAGAAGAGC CCACCAGTGT CTCCAAGTAC TTTTTCAGCG ACATCTTTAC TGAAGTTGAT 
GAATTAGAGG ACATGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:162472 ( XM_009295208.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293483.1 Danio rerio zgc:162472 (zgc:162472), transcript variant X6, mRNA.

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CloneID	ODa41968
Clone ID Related Accession (Same CDS sequence)	XM_009295208.2
Accession Version	XM_009295208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC553495 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGG ATGTCATTGA GTTTCTTGAC CCAGAACACA TGGAAGTATG TAAAGAGATC 
AACAATGAAG CAGCTCAGAC TCTTCTGACC ATCGGCAACT CGGCTCAGAT GATCCAGACC 
TCAGAAATGC CCTGCTTAAG TGGACATGAT ATTGATGTGC ATGAAGAAAT CGCTAATGAG 
GAAGTCTTCA CAGAAACGGC CATCATGTCT GATCCTTCTA TAAGCTGTTA TTCAGAGATG 
AGAAGTGATG TTGAACTTTC AAACAGTGAA ACTCAAATCC CAGAACCTCC CACTTCAATA 
GAAGAGCCAA TCAAATCACA GAATTCAATG TCTCATCCTG GTCCCGTTCA GCAAGTCTCA 
CAGGATCTGG CATCTACTTT TGTTCCTTCT TCAACAAGAG GCCGTTTCGC CAAACCCAAG 
CCCAATATTG GTCTGAAAAG CAGACGAGCT CCTCAGCAGC AGATCAGTTC AGAACTTCCT 
GCAGATTCTG TGGAGAACTC AAAGAGTCTG ACTTCGGCAG AACAGGATGA AAAACAAAAG 
GCATCCATGC AAGAGATTGC AGATGAACTG TCAGACTCCT CTAACATACA CCCTGAGGTC 
CAGCAGCCGG GTACAGTATC TGATAGAAGA GAAGATGCTC CTGAAAGACT GACTGGAGGT 
GATAGTGGAT CTGCTGTGTC ATTAAAGAGA AAAGATGAAG ACGAAATCAC AGAGGAGAAG 
ATAAAAGAAC ACGAAGAGGC TAAATCAGAT CCAGTGCTTA TTAATAGTGA GAGTGAGTCT 
CAGAGCACAC TAGACGAGGC TCCTAAACCT GTCAGGAGGA ACAGGGGACC CAAACCTAAA 
CCAAACCTGA CTCAAGCATC CAGAAAAAAA CACACACAAA CACAACTCAA CACTCCTTCA 
CTCACAACAG GAATAACTTT CACTAAAAGT CCTGAAGAGG GCGCAGTTAT TACCACAGAA 
CCTGATGTTT CTCAAAGAGC AACACAACAG GAGGTTACAT TGAGGCTTGA ATCCACACAG 
GCTGTCAAAG ATATTTCTCC AGTTGTTTCT GAAGACAATT CCAGACCGAA TACTCTGGTC 
AGATTAGATG AAGAACAAAA CATAACGGCT CCAGATGAAG CTTTTGAAGA CAGCAGTGTG 
AAACATTCAA GATGTGAATC TAAAGATGCT TCTGAGGAAT CCATGTTTAT ACTGTCTCTG 
ACAGAAATCC CACCCGCTTT ACAACAGGGG GCGTGTCTTA GGACAGAACC CCTCCCACCC 
ACAATTGAGC CCGGCTTGCA TTCTCATGGT CAAAGTGTGA ATGAGAGCAA GGAAGTGTCT 
CACCTTCTGC TCACAGATGC TTTAGTTCTT GTGTCTGAAG AAGAGGAGAA GGAAGGAGAA 
GAAGAAGACA AATCCAGGAG AGGACAACTG AAACGCAAGA CACCAGCCTC GACATCTCAA 
GAGAGTACCA AAAGTGAGAG TCAAGCAGAA GATTGTGTCG TGGAAGTTTC AGAAAGCCCT 
GTGGCAGAAG AGACTGATGA ACATCTGGAA AAGAAAAGAA AACCACCGGA GAGAACAAGG 
AGAGCAAAGC TGCAGGTTAA ACCCATCCCT GTTATGAGAA AAAACACTCG AGGCGTTAAA 
GAGGACACAG CAGCTCTTAC CTTAAAAGAA ACTCCTTCAT TACCCATTTC AACAGCAGAG 
ACAAAACCAG AGCAAACTGA CCCCACCGTC ATTTCAACAT CAGCAAGCAG TGATAATATT 
CCCTCCTCTG AAGAGTCTGT CCAGGAAGAA TCTTCTCAAG GCACTGTGGG ACAGGTGGAC 
ATCACAGTAA AAGAGACAGA TGAGTTGGCT AGTGGATCAG ACTCACAAAC TGCTACTCAA 
ATCACGCCTG TTGCCACAAG TGGGCCACTT ACAAGGCCTG GTAGAAAACC CAAAGGCTTC 
TTATCCTTCA TTTCAACAAA AAGTACACAA GGAGCACCAA CACGTCATCG AGGATCCAAA 
CCAGGAGTCA ACACTGCCCG TCCTGACAGA AAACAGGTAG CGAGTGCATC AGCAAACCTT 
GGAGTAAAGC GAGCTTTACC AACTCCTGCT TCCACCACTG AGCAGCGCTC AGAAGAAGAG 
CCCACCAGTG TCTCCAAGTA CTTTTTCAGC GACATCTTTA CTGAAGTTGA TGAATTAGAG 
GACATGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293483.1
CDS	107..4858
Translation	MMRRSRISVRPNVKPTGRAPTASREASAENAQAFQSPADSAPSEGSEVTAEKLNTDPSIAAPEQTNEDASQSSSPSDQLEDTRRGEDASSKSSNALDSVSTSVTSGPQRRKRFTALPNLAKPRASQVSAKTPKSPIKSPVKPVTPIEPEKSTTNADIPAPVHNPRVPARRRPSGGSRPAKSQLIPPESLQKDGESQKNGGLLEVVVPLTVQEGGVGTISPPDTVSDVNENRPTPPTVEEMVVQQESDSGAPPDQSQTDLLKEKLKKLKSTSKIIKSLTAVNDPADMIRLAQARKLRELLKKEMVKDKEDKRKLKMGLKERKAPKDHTKMTMRDLIYYLPVSNPMKSFTEEEQKASETVLDDSPTPISSNTPDPPAVEEPVVEDADPEEDEERTEEAQEEDKEPLLVPRVKVAEDGSLIIDEESLTVQVLRANGPNPAEDRDPIFERGSTTTYSSFRKGTYTKPWSNGETEMFFLAISMVGTDFSMIGQLFPHRGRLEIKNKFKKEEKNNAWRIDKAFKEKRRLDLDFFKKLMEQILNDEEEKKNKSKEHFKLAKAKRKVRVAKRKGMDSSEDSDSDVVAGEKENEDLSNDGESDVTPKRRRGRAAKNTDRDIKRIYEEVDNPEDCENVTSDTQSPDDRLKDSESSKNINKSPSIKPALLKGRPQKLIPNISRRWGERRPKNNIQENRTSAKDEDPLKVPSASKEEKDKKQASVIIDLIDLDEEPDLSAVQEQIFNKPTRSGRIPKLSQHVIQAAAEEEEDEEEPSDPPTSSKVCVPSPGCRAKIKPGPRLKQGMQRRGKSRLVTLLASGAEEDDDEEREEAEGCILSQEDFPSNSEEENQAFVPMSLRPQDHFDSEVVETMEEDVIEFLDPEHMEVCKEINNEAAQTLLTIGNSAQMIQTSEMPCLSGHDIDVHEEIANEEVFTETAIMSDPSISCYSEMRSDVELSNSETQIPEPPTSIEEPIKSQNSMSHPGPVQQVSQDLASTFVPSSTRGRFAKPKPNIGLKSRRAPQQQISSELPADSVENSKSLTSAEQDEKQKASMQEIADELSDSSNIHPEVQQPGTVSDRREDAPERLTGGDSGSAVSLKRKDEDEITEEKIKEHEEAKSDPVLINSESESQSTLDEAPKPVRRNRGPKPKPNLTQASRKKHTQTQLNTPSLTTGITFTKSPEEGAVITTEPDVSQRATQQEVTLRLESTQAVKDISPVVSEDNSRPNTLVRLDEEQNITAPDEAFEDSSVKHSRCESKDASEESMFILSLTEIPPALQQGACLRTEPLPPTIEPGLHSHGQSVNESKEVSHLLLTDALVLVSEEEEKEGEEEDKSRRGQLKRKTPASTSQESTKSESQAEDCVVEVSESPVAEETDEHLEKKRKPPERTRRAKLQVKPIPVMRKNTRGVKEDTAALTLKETPSLPISTAETKPEQTDPTVISTSASSDNIPSSEESVQEESSQGTVGQVDITVKETDELASGSDSQTATQITPVATSGPLTRPGRKPKGFLSFISTKSTQGAPTRHRGSKPGVNTARPDRKQVASASANLGVKRALPTPASTTEQRSEEEPTSVSKYFFSDIFTEVDELEDMD

Target ORF information:

RefSeq Version	XM_009295208.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:162472 (zgc:162472), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295208.2

ORF Insert Sequence:

ATGATGCGCA GGTCCAGAAT CAGTGTTCGG CCAAATGTAA AGCCCACAGG CCGAGCTCCG 
ACAGCTTCTC GAGAAGCATC TGCGGAGAAT GCTCAGGCTT TCCAGAGTCC CGCTGACTCT 
GCTCCATCTG AGGGTTCAGA GGTCACAGCT GAAAAACTGA ATACTGACCC GTCTATAGCA 
GCGCCGGAAC AAACCAACGA GGACGCATCT CAGAGCAGCA GCCCTAGTGA CCAGTTGGAG 
GACACTAGAC GTGGAGAGGA TGCATCATCT AAAAGTTCAA ATGCCCTGGA TTCAGTAAGC 
ACCTCTGTGA CATCAGGCCC ACAGAGGAGG AAACGCTTCA CAGCTTTACC CAACCTGGCC 
AAACCACGAG CCTCCCAAGT CTCCGCTAAG ACTCCGAAAT CCCCAATAAA ATCTCCTGTA 
AAACCAGTGA CACCTATTGA GCCTGAAAAA TCAACCACCA ATGCAGACAT ACCTGCGCCT 
GTCCACAATC CCAGGGTTCC TGCAAGACGG AGACCTTCAG GAGGAAGCAG ACCAGCCAAA 
AGTCAACTCA TTCCTCCAGA ATCACTGCAG AAGGATGGAG AGAGCCAAAA AAATGGGGGA 
TTACTAGAAG TTGTTGTGCC GTTGACCGTC CAGGAAGGGG GCGTTGGGAC GATTTCTCCA 
CCTGATACTG TTTCGGATGT AAATGAAAAC CGTCCGACTC CTCCTACTGT GGAAGAGATG 
GTTGTTCAAC AGGAGAGTGA TTCTGGAGCA CCACCTGACC AAAGCCAAAC TGATCTGTTG 
AAAGAGAAAC TTAAAAAACT GAAATCAACA TCAAAGATCA TCAAATCCTT GACTGCTGTG 
AACGACCCTG CAGACATGAT TCGATTAGCG CAGGCTCGGA AACTTCGGGA GCTTCTGAAA 
AAAGAAATGG TCAAAGACAA GGAAGACAAG AGGAAGCTCA AGATGGGACT TAAAGAACGC 
AAAGCACCTA AAGATCACAC CAAAATGACC ATGAGAGACC TGATCTATTA CCTGCCCGTC 
TCCAACCCAA TGAAGTCTTT TACAGAAGAG GAGCAGAAAG CATCAGAGAC AGTATTAGAT 
GACTCTCCTA CACCAATTTC TTCCAATACT CCAGATCCTC CAGCCGTTGA GGAGCCGGTG 
GTAGAAGATG CTGATCCCGA GGAAGATGAA GAGAGAACAG AAGAAGCTCA GGAGGAGGAT 
AAAGAGCCTC TGCTGGTGCC CAGAGTGAAG GTGGCTGAGG ATGGATCTCT GATTATTGAC 
GAGGAGAGTT TAACAGTGCA GGTATTGAGA GCTAATGGGC CCAATCCAGC AGAAGACAGA 
GATCCTATAT TTGAGCGTGG CTCCACCACC ACCTACTCCA GCTTTAGGAA AGGCACCTAC 
ACCAAACCAT GGTCCAACGG AGAAACTGAG ATGTTTTTCC TGGCCATCAG CATGGTTGGG 
ACTGATTTCT CCATGATCGG CCAACTCTTT CCACACCGAG GTCGATTGGA GATCAAGAAC 
AAGTTCAAGA AAGAGGAGAA AAATAATGCA TGGAGAATAG ATAAAGCTTT CAAGGAGAAG 
CGACGTTTGG ATCTAGATTT CTTCAAAAAG TTAATGGAGC AGATTCTAAA TGACGAGGAA 
GAGAAAAAGA ACAAAAGCAA AGAGCATTTC AAGTTGGCAA AAGCAAAAAG GAAAGTGAGA 
GTGGCTAAAA GAAAGGGAAT GGACTCTTCA GAGGATTCAG ACAGTGATGT GGTGGCAGGA 
GAAAAGGAGA ATGAAGATCT TTCAAATGAT GGAGAAAGTG ATGTCACTCC AAAGAGACGC 
AGGGGACGAG CAGCGAAAAA TACAGACAGG GACATTAAGA GGATATATGA AGAGGTAGAT 
AACCCTGAGG ACTGTGAAAA TGTTACTTCA GACACTCAGT CACCGGATGA CAGGTTAAAG 
GACTCAGAAT CATCAAAGAA CATAAACAAG AGTCCTTCTA TTAAACCTGC TCTGCTGAAG 
GGTCGACCTC AGAAACTGAT CCCGAACATC AGCCGCAGAT GGGGCGAACG ACGGCCCAAA 
AACAACATCC AGGAAAACAG GACTTCGGCT AAAGATGAAG ATCCTCTCAA GGTGCCTTCC 
GCCTCAAAAG AAGAGAAAGA CAAGAAACAG GCTTCAGTTA TCATTGATCT GATTGATTTA 
GATGAAGAAC CGGACCTGAG TGCTGTGCAA GAACAGATAT TCAATAAACC AACCAGGTCA 
GGAAGGATCC CCAAGCTCTC TCAGCATGTG ATACAGGCAG CGGCAGAGGA GGAGGAAGAT 
GAGGAAGAGC CCTCTGATCC CCCTACCTCT TCCAAAGTTT GTGTTCCATC ACCTGGCTGT 
AGGGCTAAAA TAAAACCAGG CCCCAGACTA AAACAGGGCA TGCAGAGGAG AGGGAAATCT 
AGACTGGTGA CTTTACTGGC CTCTGGGGCT GAAGAAGATG ATGATGAAGA GAGAGAGGAA 
GCAGAGGGTT GCATCCTGAG CCAGGAGGAC TTTCCTTCAA ACTCTGAAGA GGAAAACCAG 
GCATTTGTGC CAATGAGTCT ACGACCTCAA GATCATTTTG ATTCAGAGGT GGTAGAAACC 
ATGGAAGAGG ATGTCATTGA GTTTCTTGAC CCAGAACACA TGGAAGTATG TAAAGAGATC 
AACAATGAAG CAGCTCAGAC TCTTCTGACC ATCGGCAACT CGGCTCAGAT GATCCAGACC 
TCAGAAATGC CCTGCTTAAG TGGACATGAT ATTGATGTGC ATGAAGAAAT CGCTAATGAG 
GAAGTCTTCA CAGAAACGGC CATCATGTCT GATCCTTCTA TAAGCTGTTA TTCAGAGATG 
AGAAGTGATG TTGAACTTTC AAACAGTGAA ACTCAAATCC CAGAACCTCC CACTTCAATA 
GAAGAGCCAA TCAAATCACA GAATTCAATG TCTCATCCTG GTCCCGTTCA GCAAGTCTCA 
CAGGATCTGG CATCTACTTT TGTTCCTTCT TCAACAAGAG GCCGTTTCGC CAAACCCAAG 
CCCAATATTG GTCTGAAAAG CAGACGAGCT CCTCAGCAGC AGATCAGTTC AGAACTTCCT 
GCAGATTCTG TGGAGAACTC AAAGAGTCTG ACTTCGGCAG AACAGGATGA AAAACAAAAG 
GCATCCATGC AAGAGATTGC AGATGAACTG TCAGACTCCT CTAACATACA CCCTGAGGTC 
CAGCAGCCGG GTACAGTATC TGATAGAAGA GAAGATGCTC CTGAAAGACT GACTGGAGGT 
GATAGTGGAT CTGCTGTGTC ATTAAAGAGA AAAGATGAAG ACGAAATCAC AGAGGAGAAG 
ATAAAAGAAC ACGAAGAGGC TAAATCAGAT CCAGTGCTTA TTAATAGTGA GAGTGAGTCT 
CAGAGCACAC TAGACGAGGC TCCTAAACCT GTCAGGAGGA ACAGGGGACC CAAACCTAAA 
CCAAACCTGA CTCAAGCATC CAGAAAAAAA CACACACAAA CACAACTCAA CACTCCTTCA 
CTCACAACAG GAATAACTTT CACTAAAAGT CCTGAAGAGG GCGCAGTTAT TACCACAGAA 
CCTGATGTTT CTCAAAGAGC AACACAACAG GAGGTTACAT TGAGGCTTGA ATCCACACAG 
GCTGTCAAAG ATATTTCTCC AGTTGTTTCT GAAGACAATT CCAGACCGAA TACTCTGGTC 
AGATTAGATG AAGAACAAAA CATAACGGCT CCAGATGAAG CTTTTGAAGA CAGCAGTGTG 
AAACATTCAA GATGTGAATC TAAAGATGCT TCTGAGGAAT CCATGTTTAT ACTGTCTCTG 
ACAGAAATCC CACCCGCTTT ACAACAGGGG GCGTGTCTTA GGACAGAACC CCTCCCACCC 
ACAATTGAGC CCGGCTTGCA TTCTCATGGT CAAAGTGTGA ATGAGAGCAA GGAAGTGTCT 
CACCTTCTGC TCACAGATGC TTTAGTTCTT GTGTCTGAAG AAGAGGAGAA GGAAGGAGAA 
GAAGAAGACA AATCCAGGAG AGGACAACTG AAACGCAAGA CACCAGCCTC GACATCTCAA 
GAGAGTACCA AAAGTGAGAG TCAAGCAGAA GATTGTGTCG TGGAAGTTTC AGAAAGCCCT 
GTGGCAGAAG AGACTGATGA ACATCTGGAA AAGAAAAGAA AACCACCGGA GAGAACAAGG 
AGAGCAAAGC TGCAGGTTAA ACCCATCCCT GTTATGAGAA AAAACACTCG AGGCGTTAAA 
GAGGACACAG CAGCTCTTAC CTTAAAAGAA ACTCCTTCAT TACCCATTTC AACAGCAGAG 
ACAAAACCAG AGCAAACTGA CCCCACCGTC ATTTCAACAT CAGCAAGCAG TGATAATATT 
CCCTCCTCTG AAGAGTCTGT CCAGGAAGAA TCTTCTCAAG GCACTGTGGG ACAGGTGGAC 
ATCACAGTAA AAGAGACAGA TGAGTTGGCT AGTGGATCAG ACTCACAAAC TGCTACTCAA 
ATCACGCCTG TTGCCACAAG TGGGCCACTT ACAAGGCCTG GTAGAAAACC CAAAGGCTTC 
TTATCCTTCA TTTCAACAAA AAGTACACAA GGAGCACCAA CACGTCATCG AGGATCCAAA 
CCAGGAGTCA ACACTGCCCG TCCTGACAGA AAACAGGTAG CGAGTGCATC AGCAAACCTT 
GGAGTAAAGC GAGCTTTACC AACTCCTGCT TCCACCACTG AGCAGCGCTC AGAAGAAGAG 
CCCACCAGTG TCTCCAAGTA CTTTTTCAGC GACATCTTTA CTGAAGTTGA TGAATTAGAG 
GACATGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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zgc:162472 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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