chd5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chd5
Entrez Gene ID	553422
Full Name	chromodomain helicase DNA binding protein 5
General protein information	Preferred Names chromodomain helicase DNA binding protein 5 Names chromodomain-helicase-DNA-binding protein 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306105.2	XP_009304380.1	chromodomain-helicase-DNA-binding protein 5 isoform X1
XM_009306106.2	XP_009304381.1	chromodomain-helicase-DNA-binding protein 5 isoform X2
XM_003199497.4	XP_003199545.2	chromodomain-helicase-DNA-binding protein 5 isoform X3
XM_009306105.3	XP_009304380.1	chromodomain-helicase-DNA-binding protein 5 isoform X1
XM_021479856.1	XP_021335531.1	chromodomain-helicase-DNA-binding protein 5 isoform X4
XM_003199497.5	XP_003199545.2	chromodomain-helicase-DNA-binding protein 5 isoform X3
XM_009306106.3	XP_009304381.1	chromodomain-helicase-DNA-binding protein 5 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHD5	XP_002694217.3
Mus musculus (house mouse)	Chd5	NP_001074845.1
Drosophila melanogaster (fruit fly)	Mi-2	NP_001014591.1
Homo sapiens (human)	CHD5	NP_056372.1
Rattus norvegicus (Norway rat)	Chd5	NP_001258155.1
Gallus gallus (chicken)	CHD5	XP_003642567.2
Danio rerio (zebrafish)	chd5	XP_003199545.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012009	XP_320523.4
Canis lupus familiaris (dog)	CHD5	XP_005620504.1
Xenopus tropicalis (tropical clawed frog)	chd5	XP_002937591.2

Gene Ontology

The following chd5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chd5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148023	XM_021479856.1 Latest version!	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa17167	XM_003199497.5 Latest version!	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41945	XM_009306105.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa41946	XM_009306106.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa41945	XM_009306105.2	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa17167	XM_003199497.4	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41946	XM_009306106.2	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chd5 ( XM_021479856.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335531.1 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X4, mRNA.

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CloneID	ODa148023
Clone ID Related Accession (Same CDS sequence)	XM_021479856.1
Accession Version	XM_021479856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGGAC 
ACGCCAATTA AGTTTCATGT CCTGCTGACA TCATATGAAC TGATCACCAT TGATCAGGCT 
ATACTCGGCT CCATTACCTG GGCCTGTTTG GTTGTGGATG AAGCCCACCG ACTGAAGAAT 
AACCAGTCAA AGTTTTTCAG AATTCTCAAT GGCTACAAAA TCTACTATAA GCTGCTCTTG 
ACTGGAACCC CTTTGCAGAA CAATCTGGAA GAGCTTTTTC ACCTGCTCAA CTTCCTCACC 
CCGGAGCGCT TCAATAACCT GGAGGGCTTT CTGGAGGAGT TTGCGGACAT CTCGAAAGAG 
GACCAGATTA AGAAACTTCA CGACCTGCTG GGGCCACACA TGCTCAGGAG ATTGAAAGCT 
GATGTGTTTA AGAACATGCC AGCAAAAACC GAGCTCATCG TGCGAGTTGA ACTCAGCCCT 
ATGCAGAAGA AATACTACAA GTTTATTTTA ACAAGGAACT TTGAGGCTTT GAATTCCAAA 
GGAGGAGGGA ACCAGGTGTC CCTTCTGAAC ATCATGATGG ACCTGAAGAA ATGCTGTAAC 
CATCCCTACC TGTTTCCAGT GGCTGCAGTG GAAGCTCCGG TGTTACCCAA TGGCTCTTAT 
GATGGTAACC TTCTGGTAAA ATCCTCAGGG AAACTCACCC TGCTTCAGAA AATGCTCATA 
AAGCTGAAAG ATGGAGGACA CAGGGTTCTC ATCTTCTCTC AGATGACAAA GATGCTGGAT 
TTGCTGGAGG ACTTCCTGGA GTTCGAGGGG TATAAATACG AGCGCATTGA CGGGGGCATC 
ACAGGCGGGC TGCGACAGGA GGCCATCGAT CGCTTTAACG CACCCGGCGC TCAGCAGTTC 
TGTTTTCTGC TGTCCACACG TGCTGGAGGA TTGGGTATCA ATCTGGCAAC CGCAGACACA 
GTAATCATAT ATGACTCTGA CTGGAATCCG CACAATGACA TTCAGGCATT CAGCAGGGCG 
CATCGAATTG GTCAAAATAA AAAGGTGATG ATCTATCGCT TTGTAACTCG GGCATCCGTA 
GAGGAGCGGA TCACCCAGGT GGCCAAGCGA AAGATGATGC TGACCCACCT GGTGGTGCGG 
CCCGGCTTGG GCTCCAAGAC GGGCTCTATG TCCAAGCAAG AGCTAGACGA CATCCTCAAA 
TTTGGCACTG AAGAGCTTTT TAAAGACGAC GTGGAGGCCG CCAGAACGAT GGGAGACAAT 
AAAGAAGGAG AGGAGGTCAG TGTGATCCAC TATGACGATA ATGCCATCTC CAAACTTCTG 
GACCGTAGTC AAGATGCCAC CGAAGACACT GAGATCCAGA ACATGAACGA GTACCTGAGC 
TCATTTAAAG TGGCTCAGTA TGTCGTGAGA GAAGAGGACG GAGAGGAGGA GGTCCAGAGG 
GAGATTATTA AGCAGGAGGA GAATGTTGAT CCAGACTATT GGGAGAAGCT CTTGAGGCAC 
CATTATGAGC AGCAGCAGGA GGATCTGGCT CGAAACCTGG GCAAGGGGAA ACGCATCCGC 
AAACAGGTCA ACTATAATGA CGCCTCTCAA GAGGACCAAG AGTGGCAGGA CGATCTTTCG 
GATAATCAGT CTGAGTACTC TGTCGGGTCT GAAGATGAAG ATGAAGATTT TGAGGAACGG 
CCTGAGGGTG GACGCAGACA CTCCCGCAGA CAGCTGAAGA GTGATCGGGA CAAACCATTG 
CCGCCACTGC TGGCTAGAGT TAGTGGAAAT ATAGAGGTTT TGGGATTTAA TGCCCGGCAG 
CGAAAAGCCT TCTTGAATGC GATTATGCGC TGGGGAATGC CTCCGCAAGA TGCCTTCAAC 
TCACACTGGC TCGTCCGTGA CCTTAGAGGG AAAAGTGAGA AGGAGTTCAG GGCTTACGTG 
TCTCTGTTCA TGAGGCACCT GTGTGAGCCG GGAGCAGATG GAGCAGAGAC ATTTGCAGAT 
GGAGTCCCAA GGGAAGGCCT GTCCCGCCAG CATGTTCTCA CCCGGATCGG TGTCATGTCT 
CTGGTCCGCA AGAAGGTTCA GGAGTTTGAA CATGTGAATG GGAAGTTTAG TACACCAGAT 
CGGATTCCTG TAGGATTAGA GCTGAAAAAA CTAACAGAAA GTGTCTCGTC GGATCCAAAC 
ACCCCTGTTC CTGCCAGTCC TGTTCCAACT CAACCCAGCA CACCGGTTCC TTCAGATAAG 
ACAGAGTCAG TGTCCGGGTC TCAAGAAGAT AAGGAGATTG CAGAGCCAGA GAACACCAAA 
CCGCTGGACC CTGAGCCAGT TGTTCCAGAA CCCTCTCCTG CGCCAGAAAA GACCAATGAG 
GGAGAAGAAA CAAAAAACGG CAGTCCTGAA GAGAAACGAT TGGAGGAGAG CGAGAAAAGT 
GACACCCCTC CAGTCCAATC AGAGCCATCA GCCAATCAAG CACCGCCACC TACTAAGCAA 
TGTGAGCAGC CAGCCAATCA GACACCTTCA GACTGTGGTA AAGGAAAAGA GACTTCTCCA 
GAAAGAGGGG AAACTAAACC CAGCCCTCCA AAAACAGAGG ATAAGGAACA AAAACCAGCC 
ATTCCTGATG ATGTAAAATC TGAAGACCCT CCTGAAAACC AAGTGAATGG AGAGAAAGAT 
GTAAGAGATG AGACGGACGA GGGCCACAGA GATGACAAGA GCAGCATCAA AACTCGCTTC 
ATGTTCAACA TTGCAGATGG AGGATTCACA GAGTTGCACA CGCTATGGCA GAATGAGGAA 
CGCGCAGCTG TGTCATCTGG CAAAATATAC GACATCTGGC ATCGTCGCCA TGACTACTGG 
CTGCTGGCTG GCATCGTAAC ACATGGTTAT GCCCGCTGGC AGGACATTCA GAATGACCCA 
CGTTATGCCA TCCTCAATGA GCCTTTCAAG ACTGAAATGC ATAAAGGGAA CTACTTAGAA 
ATGAAGAACA AGTTCCTTGC CAGACGCTTT AAGCTACTGG AACAAGCTTT GGTCATCGAG 
GAGCAGCTCC GGCGTGCGGC GTACCTGAAC ATGACTCAGG ACCCCAGTCA TCCTGCCATG 
GCCCTCAACA CACGTTTTGC AGAAGTGGAG TGTCTGGCCG AGTCTCATCA GCACCTCTCC 
AAAGAGTCTC TGGCTGGTAA CAAGCCAGCC AATGCAGTGC TGCACAAAGT GTTAAATCAG 
CTGGAGGAGT TGTTAAGTGA TATGAAGGCG GATGTGACGC GTTTGCCCTC CATGCTTTCG 
CGGGTGCCAC CAGTGTCCGC GCGGCTCCAG ATGTCAGAGC GGGGCATCCT CAGCAGACTC 
ACCAGCCGGG GCAATGAGCC GCCACCTCAG CAGCCTTTCC CTCAGGGCTC TTTCGGCTGT 
TCTCAGATGT ATAATAGCAG TTTCGCCGGA GGGTTTCGTG GACCTGGAGG CACCGCCATG 
GTGAACTATA GCCAGATGCC CCTGGGGCCG TACGTCAGTG TTTCCTCAAA CGGGCCTCCT 
GCCCCTACCA GCCTCCTGGA GAAGAAGTCG AGTGACGTCC TGCGAGATGT GGTAACCCCT 
GATTTAAAAT CAGGCAAACC CAGTGATGTT ATATGCATCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335531.1
CDS	608..6595
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVVTPDLKSGKPSDVICIED

Target ORF information:

RefSeq Version	XM_021479856.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479856.1

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGGAC 
ACGCCAATTA AGTTTCATGT CCTGCTGACA TCATATGAAC TGATCACCAT TGATCAGGCT 
ATACTCGGCT CCATTACCTG GGCCTGTTTG GTTGTGGATG AAGCCCACCG ACTGAAGAAT 
AACCAGTCAA AGTTTTTCAG AATTCTCAAT GGCTACAAAA TCTACTATAA GCTGCTCTTG 
ACTGGAACCC CTTTGCAGAA CAATCTGGAA GAGCTTTTTC ACCTGCTCAA CTTCCTCACC 
CCGGAGCGCT TCAATAACCT GGAGGGCTTT CTGGAGGAGT TTGCGGACAT CTCGAAAGAG 
GACCAGATTA AGAAACTTCA CGACCTGCTG GGGCCACACA TGCTCAGGAG ATTGAAAGCT 
GATGTGTTTA AGAACATGCC AGCAAAAACC GAGCTCATCG TGCGAGTTGA ACTCAGCCCT 
ATGCAGAAGA AATACTACAA GTTTATTTTA ACAAGGAACT TTGAGGCTTT GAATTCCAAA 
GGAGGAGGGA ACCAGGTGTC CCTTCTGAAC ATCATGATGG ACCTGAAGAA ATGCTGTAAC 
CATCCCTACC TGTTTCCAGT GGCTGCAGTG GAAGCTCCGG TGTTACCCAA TGGCTCTTAT 
GATGGTAACC TTCTGGTAAA ATCCTCAGGG AAACTCACCC TGCTTCAGAA AATGCTCATA 
AAGCTGAAAG ATGGAGGACA CAGGGTTCTC ATCTTCTCTC AGATGACAAA GATGCTGGAT 
TTGCTGGAGG ACTTCCTGGA GTTCGAGGGG TATAAATACG AGCGCATTGA CGGGGGCATC 
ACAGGCGGGC TGCGACAGGA GGCCATCGAT CGCTTTAACG CACCCGGCGC TCAGCAGTTC 
TGTTTTCTGC TGTCCACACG TGCTGGAGGA TTGGGTATCA ATCTGGCAAC CGCAGACACA 
GTAATCATAT ATGACTCTGA CTGGAATCCG CACAATGACA TTCAGGCATT CAGCAGGGCG 
CATCGAATTG GTCAAAATAA AAAGGTGATG ATCTATCGCT TTGTAACTCG GGCATCCGTA 
GAGGAGCGGA TCACCCAGGT GGCCAAGCGA AAGATGATGC TGACCCACCT GGTGGTGCGG 
CCCGGCTTGG GCTCCAAGAC GGGCTCTATG TCCAAGCAAG AGCTAGACGA CATCCTCAAA 
TTTGGCACTG AAGAGCTTTT TAAAGACGAC GTGGAGGCCG CCAGAACGAT GGGAGACAAT 
AAAGAAGGAG AGGAGGTCAG TGTGATCCAC TATGACGATA ATGCCATCTC CAAACTTCTG 
GACCGTAGTC AAGATGCCAC CGAAGACACT GAGATCCAGA ACATGAACGA GTACCTGAGC 
TCATTTAAAG TGGCTCAGTA TGTCGTGAGA GAAGAGGACG GAGAGGAGGA GGTCCAGAGG 
GAGATTATTA AGCAGGAGGA GAATGTTGAT CCAGACTATT GGGAGAAGCT CTTGAGGCAC 
CATTATGAGC AGCAGCAGGA GGATCTGGCT CGAAACCTGG GCAAGGGGAA ACGCATCCGC 
AAACAGGTCA ACTATAATGA CGCCTCTCAA GAGGACCAAG AGTGGCAGGA CGATCTTTCG 
GATAATCAGT CTGAGTACTC TGTCGGGTCT GAAGATGAAG ATGAAGATTT TGAGGAACGG 
CCTGAGGGTG GACGCAGACA CTCCCGCAGA CAGCTGAAGA GTGATCGGGA CAAACCATTG 
CCGCCACTGC TGGCTAGAGT TAGTGGAAAT ATAGAGGTTT TGGGATTTAA TGCCCGGCAG 
CGAAAAGCCT TCTTGAATGC GATTATGCGC TGGGGAATGC CTCCGCAAGA TGCCTTCAAC 
TCACACTGGC TCGTCCGTGA CCTTAGAGGG AAAAGTGAGA AGGAGTTCAG GGCTTACGTG 
TCTCTGTTCA TGAGGCACCT GTGTGAGCCG GGAGCAGATG GAGCAGAGAC ATTTGCAGAT 
GGAGTCCCAA GGGAAGGCCT GTCCCGCCAG CATGTTCTCA CCCGGATCGG TGTCATGTCT 
CTGGTCCGCA AGAAGGTTCA GGAGTTTGAA CATGTGAATG GGAAGTTTAG TACACCAGAT 
CGGATTCCTG TAGGATTAGA GCTGAAAAAA CTAACAGAAA GTGTCTCGTC GGATCCAAAC 
ACCCCTGTTC CTGCCAGTCC TGTTCCAACT CAACCCAGCA CACCGGTTCC TTCAGATAAG 
ACAGAGTCAG TGTCCGGGTC TCAAGAAGAT AAGGAGATTG CAGAGCCAGA GAACACCAAA 
CCGCTGGACC CTGAGCCAGT TGTTCCAGAA CCCTCTCCTG CGCCAGAAAA GACCAATGAG 
GGAGAAGAAA CAAAAAACGG CAGTCCTGAA GAGAAACGAT TGGAGGAGAG CGAGAAAAGT 
GACACCCCTC CAGTCCAATC AGAGCCATCA GCCAATCAAG CACCGCCACC TACTAAGCAA 
TGTGAGCAGC CAGCCAATCA GACACCTTCA GACTGTGGTA AAGGAAAAGA GACTTCTCCA 
GAAAGAGGGG AAACTAAACC CAGCCCTCCA AAAACAGAGG ATAAGGAACA AAAACCAGCC 
ATTCCTGATG ATGTAAAATC TGAAGACCCT CCTGAAAACC AAGTGAATGG AGAGAAAGAT 
GTAAGAGATG AGACGGACGA GGGCCACAGA GATGACAAGA GCAGCATCAA AACTCGCTTC 
ATGTTCAACA TTGCAGATGG AGGATTCACA GAGTTGCACA CGCTATGGCA GAATGAGGAA 
CGCGCAGCTG TGTCATCTGG CAAAATATAC GACATCTGGC ATCGTCGCCA TGACTACTGG 
CTGCTGGCTG GCATCGTAAC ACATGGTTAT GCCCGCTGGC AGGACATTCA GAATGACCCA 
CGTTATGCCA TCCTCAATGA GCCTTTCAAG ACTGAAATGC ATAAAGGGAA CTACTTAGAA 
ATGAAGAACA AGTTCCTTGC CAGACGCTTT AAGCTACTGG AACAAGCTTT GGTCATCGAG 
GAGCAGCTCC GGCGTGCGGC GTACCTGAAC ATGACTCAGG ACCCCAGTCA TCCTGCCATG 
GCCCTCAACA CACGTTTTGC AGAAGTGGAG TGTCTGGCCG AGTCTCATCA GCACCTCTCC 
AAAGAGTCTC TGGCTGGTAA CAAGCCAGCC AATGCAGTGC TGCACAAAGT GTTAAATCAG 
CTGGAGGAGT TGTTAAGTGA TATGAAGGCG GATGTGACGC GTTTGCCCTC CATGCTTTCG 
CGGGTGCCAC CAGTGTCCGC GCGGCTCCAG ATGTCAGAGC GGGGCATCCT CAGCAGACTC 
ACCAGCCGGG GCAATGAGCC GCCACCTCAG CAGCCTTTCC CTCAGGGCTC TTTCGGCTGT 
TCTCAGATGT ATAATAGCAG TTTCGCCGGA GGGTTTCGTG GACCTGGAGG CACCGCCATG 
GTGAACTATA GCCAGATGCC CCTGGGGCCG TACGTCAGTG TTTCCTCAAA CGGGCCTCCT 
GCCCCTACCA GCCTCCTGGA GAAGAAGTCG AGTGACGTCC TGCGAGATGT GGTAACCCCT 
GATTTAAAAT CAGGCAAACC CAGTGATGTT ATATGCATCG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_003199497.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199545.2 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.

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CloneID	ODa17167
Clone ID Related Accession (Same CDS sequence)	XM_003199497.4 , XM_003199497.5
Accession Version	XM_003199497.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003199497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGAGAC 
AATAAAGAAG GAGAGGAGGT CAGTGTGATC CACTATGACG ATAATGCCAT CTCCAAACTT 
CTGGACCGTA GTCAAGATGC CACCGAAGAC ACTGAGATCC AGAACATGAA CGAGTACCTG 
AGCTCATTTA AAGTGGCTCA GTATGTCGTG AGAGAAGAGG ACGGAGAGGA GGAGGTCCAG 
AGGGAGATTA TTAAGCAGGA GGAGAATGTT GATCCAGACT ATTGGGAGAA GCTCTTGAGG 
CACCATTATG AGCAGCAGCA GGAGGATCTG GCTCGAAACC TGGGCAAGGG GAAACGCATC 
CGCAAACAGG TCAACTATAA TGACGCCTCT CAAGAGGACC AAGAGTGGCA GGACGATCTT 
TCGGATAATC AGTCTGAGTA CTCTGTCGGG TCTGAAGATG AAGATGAAGA TTTTGAGGAA 
CGGCCTGAGG GTGGACGCAG ACACTCCCGC AGACAGCTGA AGAGTGATCG GGACAAACCA 
TTGCCGCCAC TGCTGGCTAG AGTTAGTGGA AATATAGAGG TTTTGGGATT TAATGCCCGG 
CAGCGAAAAG CCTTCTTGAA TGCGATTATG CGCTGGGGAA TGCCTCCGCA AGATGCCTTC 
AACTCACACT GGCTCGTCCG TGACCTTAGA GGGAAAAGTG AGAAGGAGTT CAGGGCTTAC 
GTGTCTCTGT TCATGAGGCA CCTGTGTGAG CCGGGAGCAG ATGGAGCAGA GACATTTGCA 
GATGGAGTCC CAAGGGAAGG CCTGTCCCGC CAGCATGTTC TCACCCGGAT CGGTGTCATG 
TCTCTGGTCC GCAAGAAGGT TCAGGAGTTT GAACATGTGA ATGGGAAGTT TAGTACACCA 
GATCGGATTC CTGTAGGATT AGAGCTGAAA AAACTAACAG AAAGTGTCTC GTCGGATCCA 
AACACCCCTG TTCCTGCCAG TCCTGTTCCA ACTCAACCCA GCACACCGGT TCCTTCAGAT 
AAGACAGAGT CAGTGTCCGG GTCTCAAGAA GATAAGGAGA TTGCAGAGCC AGAGAACACC 
AAACCGCTGG ACCCTGAGCC AGTTGTTCCA GAACCCTCTC CTGCGCCAGA AAAGACCAAT 
GAGGGAGAAG AAACAAAAAA CGGCAGTCCT GAAGAGAAAC GATTGGAGGA GAGCGAGAAA 
AGTGACACCC CTCCAGTCCA ATCAGAGCCA TCAGCCAATC AAGCACCGCC ACCTACTAAG 
CAATGTGAGC AGCCAGCCAA TCAGACACCT TCAGACTGTG GTAAAGGAAA AGAGACTTCT 
CCAGAAAGAG GGGAAACTAA ACCCAGCCCT CCAAAAACAG AGGATAAGGA ACAAAAACCA 
GCCATTCCTG ATGATGTAAA ATCTGAAGAC CCTCCTGAAA ACCAAGTGAA TGGAGAGAAA 
GATGTAAGAG ATGAGACGGA CGAGGGCCAC AGAGATGACA AGAGCAGCAT CAAAACTCGC 
TTCATGTTCA ACATTGCAGA TGGAGGATTC ACAGAGTTGC ACACGCTATG GCAGAATGAG 
GAACGCGCAG CTGTGTCATC TGGCAAAATA TACGACATCT GGCATCGTCG CCATGACTAC 
TGGCTGCTGG CTGGCATCGT AACACATGGT TATGCCCGCT GGCAGGACAT TCAGAATGAC 
CCACGTTATG CCATCCTCAA TGAGCCTTTC AAGACTGAAA TGCATAAAGG GAACTACTTA 
GAAATGAAGA ACAAGTTCCT TGCCAGACGC TTTAAGCTAC TGGAACAAGC TTTGGTCATC 
GAGGAGCAGC TCCGGCGTGC GGCGTACCTG AACATGACTC AGGACCCCAG TCATCCTGCC 
ATGGCCCTCA ACACACGTTT TGCAGAAGTG GAGTGTCTGG CCGAGTCTCA TCAGCACCTC 
TCCAAAGAGT CTCTGGCTGG TAACAAGCCA GCCAATGCAG TGCTGCACAA AGTGTTAAAT 
CAGCTGGAGG AGTTGTTAAG TGATATGAAG GCGGATGTGA CGCGTTTGCC CTCCATGCTT 
TCGCGGGTGC CACCAGTGTC CGCGCGGCTC CAGATGTCAG AGCGGGGCAT CCTCAGCAGA 
CTCACCAGCC GGGGCAATGA GCCGCCACCT CAGCAGCCTT TCCCTCAGGG CTCTTTCGGC 
TGTTCTCAGA TGTATAATAG CAGTTTCGCC GGAGGGTTTC GTGGACCTGG AGGCACCGCC 
ATGGTGAACT ATAGCCAGAT GCCCCTGGGG CCGTACGTCA GTGTTTCCTC AAACGGGCCT 
CCTGCCCCTA CCAGCCTCCT GGAGAAGAAG TCGAGTGACG TCCTGCGAGA TGTGGTAACC 
CCTGATTTAA AATCAGGCAA ACCCAGTGAT GTTATATGCA TCGAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199545.2
CDS	253..6243
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVVTPDLKSGKPSDVICIED

Target ORF information:

RefSeq Version	XM_003199497.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199497.4

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGAGAC 
AATAAAGAAG GAGAGGAGGT CAGTGTGATC CACTATGACG ATAATGCCAT CTCCAAACTT 
CTGGACCGTA GTCAAGATGC CACCGAAGAC ACTGAGATCC AGAACATGAA CGAGTACCTG 
AGCTCATTTA AAGTGGCTCA GTATGTCGTG AGAGAAGAGG ACGGAGAGGA GGAGGTCCAG 
AGGGAGATTA TTAAGCAGGA GGAGAATGTT GATCCAGACT ATTGGGAGAA GCTCTTGAGG 
CACCATTATG AGCAGCAGCA GGAGGATCTG GCTCGAAACC TGGGCAAGGG GAAACGCATC 
CGCAAACAGG TCAACTATAA TGACGCCTCT CAAGAGGACC AAGAGTGGCA GGACGATCTT 
TCGGATAATC AGTCTGAGTA CTCTGTCGGG TCTGAAGATG AAGATGAAGA TTTTGAGGAA 
CGGCCTGAGG GTGGACGCAG ACACTCCCGC AGACAGCTGA AGAGTGATCG GGACAAACCA 
TTGCCGCCAC TGCTGGCTAG AGTTAGTGGA AATATAGAGG TTTTGGGATT TAATGCCCGG 
CAGCGAAAAG CCTTCTTGAA TGCGATTATG CGCTGGGGAA TGCCTCCGCA AGATGCCTTC 
AACTCACACT GGCTCGTCCG TGACCTTAGA GGGAAAAGTG AGAAGGAGTT CAGGGCTTAC 
GTGTCTCTGT TCATGAGGCA CCTGTGTGAG CCGGGAGCAG ATGGAGCAGA GACATTTGCA 
GATGGAGTCC CAAGGGAAGG CCTGTCCCGC CAGCATGTTC TCACCCGGAT CGGTGTCATG 
TCTCTGGTCC GCAAGAAGGT TCAGGAGTTT GAACATGTGA ATGGGAAGTT TAGTACACCA 
GATCGGATTC CTGTAGGATT AGAGCTGAAA AAACTAACAG AAAGTGTCTC GTCGGATCCA 
AACACCCCTG TTCCTGCCAG TCCTGTTCCA ACTCAACCCA GCACACCGGT TCCTTCAGAT 
AAGACAGAGT CAGTGTCCGG GTCTCAAGAA GATAAGGAGA TTGCAGAGCC AGAGAACACC 
AAACCGCTGG ACCCTGAGCC AGTTGTTCCA GAACCCTCTC CTGCGCCAGA AAAGACCAAT 
GAGGGAGAAG AAACAAAAAA CGGCAGTCCT GAAGAGAAAC GATTGGAGGA GAGCGAGAAA 
AGTGACACCC CTCCAGTCCA ATCAGAGCCA TCAGCCAATC AAGCACCGCC ACCTACTAAG 
CAATGTGAGC AGCCAGCCAA TCAGACACCT TCAGACTGTG GTAAAGGAAA AGAGACTTCT 
CCAGAAAGAG GGGAAACTAA ACCCAGCCCT CCAAAAACAG AGGATAAGGA ACAAAAACCA 
GCCATTCCTG ATGATGTAAA ATCTGAAGAC CCTCCTGAAA ACCAAGTGAA TGGAGAGAAA 
GATGTAAGAG ATGAGACGGA CGAGGGCCAC AGAGATGACA AGAGCAGCAT CAAAACTCGC 
TTCATGTTCA ACATTGCAGA TGGAGGATTC ACAGAGTTGC ACACGCTATG GCAGAATGAG 
GAACGCGCAG CTGTGTCATC TGGCAAAATA TACGACATCT GGCATCGTCG CCATGACTAC 
TGGCTGCTGG CTGGCATCGT AACACATGGT TATGCCCGCT GGCAGGACAT TCAGAATGAC 
CCACGTTATG CCATCCTCAA TGAGCCTTTC AAGACTGAAA TGCATAAAGG GAACTACTTA 
GAAATGAAGA ACAAGTTCCT TGCCAGACGC TTTAAGCTAC TGGAACAAGC TTTGGTCATC 
GAGGAGCAGC TCCGGCGTGC GGCGTACCTG AACATGACTC AGGACCCCAG TCATCCTGCC 
ATGGCCCTCA ACACACGTTT TGCAGAAGTG GAGTGTCTGG CCGAGTCTCA TCAGCACCTC 
TCCAAAGAGT CTCTGGCTGG TAACAAGCCA GCCAATGCAG TGCTGCACAA AGTGTTAAAT 
CAGCTGGAGG AGTTGTTAAG TGATATGAAG GCGGATGTGA CGCGTTTGCC CTCCATGCTT 
TCGCGGGTGC CACCAGTGTC CGCGCGGCTC CAGATGTCAG AGCGGGGCAT CCTCAGCAGA 
CTCACCAGCC GGGGCAATGA GCCGCCACCT CAGCAGCCTT TCCCTCAGGG CTCTTTCGGC 
TGTTCTCAGA TGTATAATAG CAGTTTCGCC GGAGGGTTTC GTGGACCTGG AGGCACCGCC 
ATGGTGAACT ATAGCCAGAT GCCCCTGGGG CCGTACGTCA GTGTTTCCTC AAACGGGCCT 
CCTGCCCCTA CCAGCCTCCT GGAGAAGAAG TCGAGTGACG TCCTGCGAGA TGTGGTAACC 
CCTGATTTAA AATCAGGCAA ACCCAGTGAT GTTATATGCA TCGAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_003199497.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003199545.2 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.

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CloneID	ODa17167
Clone ID Related Accession (Same CDS sequence)	XM_003199497.4 , XM_003199497.5
Accession Version	XM_003199497.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003199497.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGAGAC 
AATAAAGAAG GAGAGGAGGT CAGTGTGATC CACTATGACG ATAATGCCAT CTCCAAACTT 
CTGGACCGTA GTCAAGATGC CACCGAAGAC ACTGAGATCC AGAACATGAA CGAGTACCTG 
AGCTCATTTA AAGTGGCTCA GTATGTCGTG AGAGAAGAGG ACGGAGAGGA GGAGGTCCAG 
AGGGAGATTA TTAAGCAGGA GGAGAATGTT GATCCAGACT ATTGGGAGAA GCTCTTGAGG 
CACCATTATG AGCAGCAGCA GGAGGATCTG GCTCGAAACC TGGGCAAGGG GAAACGCATC 
CGCAAACAGG TCAACTATAA TGACGCCTCT CAAGAGGACC AAGAGTGGCA GGACGATCTT 
TCGGATAATC AGTCTGAGTA CTCTGTCGGG TCTGAAGATG AAGATGAAGA TTTTGAGGAA 
CGGCCTGAGG GTGGACGCAG ACACTCCCGC AGACAGCTGA AGAGTGATCG GGACAAACCA 
TTGCCGCCAC TGCTGGCTAG AGTTAGTGGA AATATAGAGG TTTTGGGATT TAATGCCCGG 
CAGCGAAAAG CCTTCTTGAA TGCGATTATG CGCTGGGGAA TGCCTCCGCA AGATGCCTTC 
AACTCACACT GGCTCGTCCG TGACCTTAGA GGGAAAAGTG AGAAGGAGTT CAGGGCTTAC 
GTGTCTCTGT TCATGAGGCA CCTGTGTGAG CCGGGAGCAG ATGGAGCAGA GACATTTGCA 
GATGGAGTCC CAAGGGAAGG CCTGTCCCGC CAGCATGTTC TCACCCGGAT CGGTGTCATG 
TCTCTGGTCC GCAAGAAGGT TCAGGAGTTT GAACATGTGA ATGGGAAGTT TAGTACACCA 
GATCGGATTC CTGTAGGATT AGAGCTGAAA AAACTAACAG AAAGTGTCTC GTCGGATCCA 
AACACCCCTG TTCCTGCCAG TCCTGTTCCA ACTCAACCCA GCACACCGGT TCCTTCAGAT 
AAGACAGAGT CAGTGTCCGG GTCTCAAGAA GATAAGGAGA TTGCAGAGCC AGAGAACACC 
AAACCGCTGG ACCCTGAGCC AGTTGTTCCA GAACCCTCTC CTGCGCCAGA AAAGACCAAT 
GAGGGAGAAG AAACAAAAAA CGGCAGTCCT GAAGAGAAAC GATTGGAGGA GAGCGAGAAA 
AGTGACACCC CTCCAGTCCA ATCAGAGCCA TCAGCCAATC AAGCACCGCC ACCTACTAAG 
CAATGTGAGC AGCCAGCCAA TCAGACACCT TCAGACTGTG GTAAAGGAAA AGAGACTTCT 
CCAGAAAGAG GGGAAACTAA ACCCAGCCCT CCAAAAACAG AGGATAAGGA ACAAAAACCA 
GCCATTCCTG ATGATGTAAA ATCTGAAGAC CCTCCTGAAA ACCAAGTGAA TGGAGAGAAA 
GATGTAAGAG ATGAGACGGA CGAGGGCCAC AGAGATGACA AGAGCAGCAT CAAAACTCGC 
TTCATGTTCA ACATTGCAGA TGGAGGATTC ACAGAGTTGC ACACGCTATG GCAGAATGAG 
GAACGCGCAG CTGTGTCATC TGGCAAAATA TACGACATCT GGCATCGTCG CCATGACTAC 
TGGCTGCTGG CTGGCATCGT AACACATGGT TATGCCCGCT GGCAGGACAT TCAGAATGAC 
CCACGTTATG CCATCCTCAA TGAGCCTTTC AAGACTGAAA TGCATAAAGG GAACTACTTA 
GAAATGAAGA ACAAGTTCCT TGCCAGACGC TTTAAGCTAC TGGAACAAGC TTTGGTCATC 
GAGGAGCAGC TCCGGCGTGC GGCGTACCTG AACATGACTC AGGACCCCAG TCATCCTGCC 
ATGGCCCTCA ACACACGTTT TGCAGAAGTG GAGTGTCTGG CCGAGTCTCA TCAGCACCTC 
TCCAAAGAGT CTCTGGCTGG TAACAAGCCA GCCAATGCAG TGCTGCACAA AGTGTTAAAT 
CAGCTGGAGG AGTTGTTAAG TGATATGAAG GCGGATGTGA CGCGTTTGCC CTCCATGCTT 
TCGCGGGTGC CACCAGTGTC CGCGCGGCTC CAGATGTCAG AGCGGGGCAT CCTCAGCAGA 
CTCACCAGCC GGGGCAATGA GCCGCCACCT CAGCAGCCTT TCCCTCAGGG CTCTTTCGGC 
TGTTCTCAGA TGTATAATAG CAGTTTCGCC GGAGGGTTTC GTGGACCTGG AGGCACCGCC 
ATGGTGAACT ATAGCCAGAT GCCCCTGGGG CCGTACGTCA GTGTTTCCTC AAACGGGCCT 
CCTGCCCCTA CCAGCCTCCT GGAGAAGAAG TCGAGTGACG TCCTGCGAGA TGTGGTAACC 
CCTGATTTAA AATCAGGCAA ACCCAGTGAT GTTATATGCA TCGAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003199545.2
CDS	509..6499
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVVTPDLKSGKPSDVICIED

Target ORF information:

RefSeq Version	XM_003199497.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003199497.5

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGAGAC 
AATAAAGAAG GAGAGGAGGT CAGTGTGATC CACTATGACG ATAATGCCAT CTCCAAACTT 
CTGGACCGTA GTCAAGATGC CACCGAAGAC ACTGAGATCC AGAACATGAA CGAGTACCTG 
AGCTCATTTA AAGTGGCTCA GTATGTCGTG AGAGAAGAGG ACGGAGAGGA GGAGGTCCAG 
AGGGAGATTA TTAAGCAGGA GGAGAATGTT GATCCAGACT ATTGGGAGAA GCTCTTGAGG 
CACCATTATG AGCAGCAGCA GGAGGATCTG GCTCGAAACC TGGGCAAGGG GAAACGCATC 
CGCAAACAGG TCAACTATAA TGACGCCTCT CAAGAGGACC AAGAGTGGCA GGACGATCTT 
TCGGATAATC AGTCTGAGTA CTCTGTCGGG TCTGAAGATG AAGATGAAGA TTTTGAGGAA 
CGGCCTGAGG GTGGACGCAG ACACTCCCGC AGACAGCTGA AGAGTGATCG GGACAAACCA 
TTGCCGCCAC TGCTGGCTAG AGTTAGTGGA AATATAGAGG TTTTGGGATT TAATGCCCGG 
CAGCGAAAAG CCTTCTTGAA TGCGATTATG CGCTGGGGAA TGCCTCCGCA AGATGCCTTC 
AACTCACACT GGCTCGTCCG TGACCTTAGA GGGAAAAGTG AGAAGGAGTT CAGGGCTTAC 
GTGTCTCTGT TCATGAGGCA CCTGTGTGAG CCGGGAGCAG ATGGAGCAGA GACATTTGCA 
GATGGAGTCC CAAGGGAAGG CCTGTCCCGC CAGCATGTTC TCACCCGGAT CGGTGTCATG 
TCTCTGGTCC GCAAGAAGGT TCAGGAGTTT GAACATGTGA ATGGGAAGTT TAGTACACCA 
GATCGGATTC CTGTAGGATT AGAGCTGAAA AAACTAACAG AAAGTGTCTC GTCGGATCCA 
AACACCCCTG TTCCTGCCAG TCCTGTTCCA ACTCAACCCA GCACACCGGT TCCTTCAGAT 
AAGACAGAGT CAGTGTCCGG GTCTCAAGAA GATAAGGAGA TTGCAGAGCC AGAGAACACC 
AAACCGCTGG ACCCTGAGCC AGTTGTTCCA GAACCCTCTC CTGCGCCAGA AAAGACCAAT 
GAGGGAGAAG AAACAAAAAA CGGCAGTCCT GAAGAGAAAC GATTGGAGGA GAGCGAGAAA 
AGTGACACCC CTCCAGTCCA ATCAGAGCCA TCAGCCAATC AAGCACCGCC ACCTACTAAG 
CAATGTGAGC AGCCAGCCAA TCAGACACCT TCAGACTGTG GTAAAGGAAA AGAGACTTCT 
CCAGAAAGAG GGGAAACTAA ACCCAGCCCT CCAAAAACAG AGGATAAGGA ACAAAAACCA 
GCCATTCCTG ATGATGTAAA ATCTGAAGAC CCTCCTGAAA ACCAAGTGAA TGGAGAGAAA 
GATGTAAGAG ATGAGACGGA CGAGGGCCAC AGAGATGACA AGAGCAGCAT CAAAACTCGC 
TTCATGTTCA ACATTGCAGA TGGAGGATTC ACAGAGTTGC ACACGCTATG GCAGAATGAG 
GAACGCGCAG CTGTGTCATC TGGCAAAATA TACGACATCT GGCATCGTCG CCATGACTAC 
TGGCTGCTGG CTGGCATCGT AACACATGGT TATGCCCGCT GGCAGGACAT TCAGAATGAC 
CCACGTTATG CCATCCTCAA TGAGCCTTTC AAGACTGAAA TGCATAAAGG GAACTACTTA 
GAAATGAAGA ACAAGTTCCT TGCCAGACGC TTTAAGCTAC TGGAACAAGC TTTGGTCATC 
GAGGAGCAGC TCCGGCGTGC GGCGTACCTG AACATGACTC AGGACCCCAG TCATCCTGCC 
ATGGCCCTCA ACACACGTTT TGCAGAAGTG GAGTGTCTGG CCGAGTCTCA TCAGCACCTC 
TCCAAAGAGT CTCTGGCTGG TAACAAGCCA GCCAATGCAG TGCTGCACAA AGTGTTAAAT 
CAGCTGGAGG AGTTGTTAAG TGATATGAAG GCGGATGTGA CGCGTTTGCC CTCCATGCTT 
TCGCGGGTGC CACCAGTGTC CGCGCGGCTC CAGATGTCAG AGCGGGGCAT CCTCAGCAGA 
CTCACCAGCC GGGGCAATGA GCCGCCACCT CAGCAGCCTT TCCCTCAGGG CTCTTTCGGC 
TGTTCTCAGA TGTATAATAG CAGTTTCGCC GGAGGGTTTC GTGGACCTGG AGGCACCGCC 
ATGGTGAACT ATAGCCAGAT GCCCCTGGGG CCGTACGTCA GTGTTTCCTC AAACGGGCCT 
CCTGCCCCTA CCAGCCTCCT GGAGAAGAAG TCGAGTGACG TCCTGCGAGA TGTGGTAACC 
CCTGATTTAA AATCAGGCAA ACCCAGTGAT GTTATATGCA TCGAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_009306105.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304380.1 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.

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CloneID	ODa41945
Clone ID Related Accession (Same CDS sequence)	XM_009306105.2 , XM_009306105.3
Accession Version	XM_009306105.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306105.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGGT AACCCCTGAT TTAAAATCAG GCAAACCCAG TGATGTTATA 
TGCATCGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304380.1
CDS	255..6329
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGELKVLEMKSIVLPLCVPIAEVLNLSSTGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVVTPDLKSGKPSDVICIED

Target ORF information:

RefSeq Version	XM_009306105.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306105.2

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGGT AACCCCTGAT TTAAAATCAG GCAAACCCAG TGATGTTATA 
TGCATCGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_009306105.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304380.1 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.

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CloneID	ODa41945
Clone ID Related Accession (Same CDS sequence)	XM_009306105.2 , XM_009306105.3
Accession Version	XM_009306105.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGGT AACCCCTGAT TTAAAATCAG GCAAACCCAG TGATGTTATA 
TGCATCGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304380.1
CDS	609..6683
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGELKVLEMKSIVLPLCVPIAEVLNLSSTGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVVTPDLKSGKPSDVICIED

Target ORF information:

RefSeq Version	XM_009306105.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306105.3

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGGT AACCCCTGAT TTAAAATCAG GCAAACCCAG TGATGTTATA 
TGCATCGAGG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_009306106.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304381.1 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.

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CloneID	ODa41946
Clone ID Related Accession (Same CDS sequence)	XM_009306106.2 , XM_009306106.3
Accession Version	XM_009306106.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGTG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304381.1
CDS	255..6281
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGELKVLEMKSIVLPLCVPIAEVLNLSSTGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVWP

Target ORF information:

RefSeq Version	XM_009306106.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306106.2

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGTG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd5 ( XM_009306106.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304381.1 Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.

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CloneID	ODa41946
Clone ID Related Accession (Same CDS sequence)	XM_009306106.2 , XM_009306106.3
Accession Version	XM_009306106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGTG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304381.1
CDS	609..6635
Translation	MPGSLSNEDEGQDDMDFEDDVPDDEEGELQRSVPLSPLERFFSEEDNVKQHKKKKTSKIMEGKEPKVKKKKKKEGLPSGLDDMSDREDHGHRSGSESSTYSALKKKKKKPREKKERKMKQRKKEDDEDEDDDDDGNMKEPKSSSQLMQEWGLEDVQYAFSEDDYKTITNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGTSATAVAAAVAAAVETVNVAPPICIRSIQQISPTGPIKKAKTKEGKGPGAKKKSKPGKETKKKGKPKKSKSKAGPGGKRKKGSSSEEDFLDDFSDFDDISIHSASVRSDTSAAAKKKSSRKGRKKRKREDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEAKDDEEEEDEVAGEEEDDHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRCMCPPLKGKVQKILHWSWGDPPLPPEVPLGPDGEKVDNLAKTPLKGRPERQLFVKWAGLSYWHCSWVSELQLELYHTVMYRNYQRKNDMDEPPPYDYGSGEEELNNEKRRSKDPQYAAMEERFYRYGIKPEWLIIHRILNHSFDKDGDVQYLIKWRDLPYDQCSWEADDFDVPDYDSLKQAYWDHRNQVLGENHQPLLFRKGKRPKEEMKRREPPPDTPVVDPTIKFEQQPWYIDDTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPFLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRAIIRENEFTFEDSAVKSGRKVFRMKKDTPIKFHVLLTSYELITIDQAILGSITWACLVVDEAHRLKNNQSKFFRILNGYKIYYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSPMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGNLLVKSSGKLTLLQKMLIKLKDGGHRVLIFSQMTKMLDLLEDFLEFEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKTGSMSKQELDDILKFGTEELFKDDVEAARTMGELKVLEMKSIVLPLCVPIAEVLNLSSTGDNKEGEEVSVIHYDDNAISKLLDRSQDATEDTEIQNMNEYLSSFKVAQYVVREEDGEEEVQREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDDLSDNQSEYSVGSEDEDEDFEERPEGGRRHSRRQLKSDRDKPLPPLLARVSGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKFSTPDRIPVGLELKKLTESVSSDPNTPVPASPVPTQPSTPVPSDKTESVSGSQEDKEIAEPENTKPLDPEPVVPEPSPAPEKTNEGEETKNGSPEEKRLEESEKSDTPPVQSEPSANQAPPPTKQCEQPANQTPSDCGKGKETSPERGETKPSPPKTEDKEQKPAIPDDVKSEDPPENQVNGEKDVRDETDEGHRDDKSSIKTRFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYAILNEPFKTEMHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPSHPAMALNTRFAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRVPPVSARLQMSERGILSRLTSRGNEPPPQQPFPQGSFGCSQMYNSSFAGGFRGPGGTAMVNYSQMPLGPYVSVSSNGPPAPTSLLEKKSSDVLRDVWP

Target ORF information:

RefSeq Version	XM_009306106.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 5 (chd5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306106.3

ORF Insert Sequence:

ATGCCCGGAT CGCTCAGCAA TGAAGATGAG GGGCAAGACG ATATGGATTT TGAGGACGAC 
GTGCCAGATG ATGAGGAAGG TGAACTTCAA AGAAGTGTCC CCCTGTCACC ACTAGAGAGA 
TTCTTCTCTG AAGAGGATAA TGTTAAACAA CATAAGAAGA AGAAAACCAG TAAGATAATG 
GAAGGAAAGG AGCCCAAAGT TAAGAAAAAG AAGAAAAAGG AGGGGCTTCC CAGCGGGCTG 
GATGACATGT CAGACAGAGA GGATCATGGC CACAGGTCGG GGAGCGAGAG CAGCACCTAC 
AGCGCCCTGA AGAAGAAAAA GAAGAAGCCC AGAGAGAAGA AAGAGAGGAA AATGAAACAG 
CGAAAGAAAG AGGATGATGA GGATGAGGAC GATGACGATG ATGGAAACAT GAAGGAACCA 
AAGTCGTCCA GTCAGCTAAT GCAAGAGTGG GGTCTCGAAG ATGTCCAGTA TGCCTTCTCG 
GAGGACGATT ATAAGACTAT CACCAACTAC AAAGCCTTCA GCCAGTTTCT TAGACCCCTT 
ATCGCCAAGA AGAACCCCAA GATCCCCATG TCAAAAATGA TGACAGTGCT TGGAGCAAAG 
TGGAGAGAGT TTAGTGCAAA CAACCCGTTC AAAGGCACCT CTGCAACTGC TGTGGCGGCT 
GCTGTGGCCG CTGCCGTGGA AACTGTCAAT GTGGCTCCAC CCATCTGTAT TAGAAGTATC 
CAACAGATCT CTCCAACTGG GCCAATCAAA AAAGCCAAAA CCAAAGAGGG AAAGGGGCCT 
GGTGCTAAAA AAAAGAGCAA GCCAGGAAAA GAAACAAAAA AGAAAGGAAA ACCGAAGAAG 
TCCAAATCGA AAGCAGGCCC GGGTGGAAAA AGGAAAAAAG GCTCTTCGAG TGAGGAGGAT 
TTCCTGGATG ACTTTTCAGA CTTTGATGAC ATCAGCATTC ACAGTGCCTC TGTACGCTCA 
GACACTTCAG CGGCAGCAAA AAAGAAGTCA AGTCGCAAAG GACGAAAAAA AAGAAAAAGA 
GAGGATGGAG ACGGCTACGA GACAGACCAT CAAGACTACT GTGAGGTTTG TCAGCAGGGA 
GGAGAAATCA TTCTGTGCGA CACCTGTCCC AGAGCATATC ACCTGGTGTG TCTGGATCCA 
GAGCTGGAAA AAGCCCCCGA GGGCAAATGG AGCTGCCCCC ACTGTGAAAA AGAAGGGATT 
CAGTGGGAGG CCAAAGATGA CGAAGAGGAG GAAGATGAGG TTGCAGGTGA AGAGGAAGAT 
GACCATATGG AATTCTGCAG AGTGTGTAAG GATGGAGGAG AGCTGCTGTG TTGTGACACA 
TGTCCTTCCT CCTACCACAT CCACTGTCTC AACCCACCAC TTCCTGAGAT TCCCAATGGA 
GAGTGGCTGT GCCCACGATG CATGTGTCCA CCTCTAAAAG GCAAAGTCCA GAAGATTCTG 
CATTGGTCGT GGGGCGATCC TCCTCTGCCA CCTGAGGTTC CCCTTGGGCC GGATGGGGAG 
AAGGTGGACA ATCTGGCCAA AACACCACTT AAAGGCCGTC CAGAGAGGCA GCTGTTTGTA 
AAATGGGCTG GCCTGTCCTA CTGGCACTGC TCCTGGGTCA GCGAACTACA GTTGGAGCTG 
TATCACACAG TAATGTACCG GAACTACCAG CGCAAGAATG ACATGGATGA ACCGCCACCG 
TACGACTACG GCTCTGGGGA GGAGGAGCTT AATAATGAGA AGAGGCGGAG CAAAGACCCT 
CAGTATGCTG CCATGGAGGA GCGTTTCTAC CGCTACGGCA TCAAGCCAGA GTGGCTGATC 
ATTCACCGCA TCCTCAACCA CAGTTTTGAT AAGGATGGAG ATGTACAGTA CTTGATAAAG 
TGGAGAGATC TGCCGTATGA TCAGTGCAGC TGGGAGGCTG ATGACTTCGA CGTCCCAGAT 
TATGACAGCC TTAAACAAGC CTACTGGGAT CACAGAAACC AGGTGCTCGG TGAAAATCAT 
CAGCCCCTGC TGTTCAGGAA GGGAAAAAGA CCCAAAGAGG AAATGAAGAG GAGAGAGCCT 
CCACCAGACA CTCCAGTTGT GGATCCCACC ATCAAGTTTG AACAGCAGCC ATGGTACATC 
GATGACACTG GGGGAACTTT GCACCCGTAC CAGCTTGAAG GTCTGAATTG GTTGCGCTTC 
TCTTGGGCTC AAGGAACCGA CACAATCTTG GCTGATGAAA TGGGACTTGG CAAGACTGTG 
CAGACTATAG TGTTCCTGTA CTCACTCTAC AAAGAGGGTC ATTCCAAAGG GCCGTTTCTG 
GTCAGCGCGC CACTTTCCAC CATCATCAAC TGGGAGAGGG AGTTTGAAAT GTGGGCTCCA 
GATTTCTACG TGGTCACTTA CACGGGGGAC AAAGAGAGCC GGGCTATCAT ACGTGAAAAC 
GAGTTCACTT TTGAGGACAG CGCTGTCAAA TCAGGACGCA AGGTTTTCCG CATGAAGAAG 
GACACGCCAA TTAAGTTTCA TGTCCTGCTG ACATCATATG AACTGATCAC CATTGATCAG 
GCTATACTCG GCTCCATTAC CTGGGCCTGT TTGGTTGTGG ATGAAGCCCA CCGACTGAAG 
AATAACCAGT CAAAGTTTTT CAGAATTCTC AATGGCTACA AAATCTACTA TAAGCTGCTC 
TTGACTGGAA CCCCTTTGCA GAACAATCTG GAAGAGCTTT TTCACCTGCT CAACTTCCTC 
ACCCCGGAGC GCTTCAATAA CCTGGAGGGC TTTCTGGAGG AGTTTGCGGA CATCTCGAAA 
GAGGACCAGA TTAAGAAACT TCACGACCTG CTGGGGCCAC ACATGCTCAG GAGATTGAAA 
GCTGATGTGT TTAAGAACAT GCCAGCAAAA ACCGAGCTCA TCGTGCGAGT TGAACTCAGC 
CCTATGCAGA AGAAATACTA CAAGTTTATT TTAACAAGGA ACTTTGAGGC TTTGAATTCC 
AAAGGAGGAG GGAACCAGGT GTCCCTTCTG AACATCATGA TGGACCTGAA GAAATGCTGT 
AACCATCCCT ACCTGTTTCC AGTGGCTGCA GTGGAAGCTC CGGTGTTACC CAATGGCTCT 
TATGATGGTA ACCTTCTGGT AAAATCCTCA GGGAAACTCA CCCTGCTTCA GAAAATGCTC 
ATAAAGCTGA AAGATGGAGG ACACAGGGTT CTCATCTTCT CTCAGATGAC AAAGATGCTG 
GATTTGCTGG AGGACTTCCT GGAGTTCGAG GGGTATAAAT ACGAGCGCAT TGACGGGGGC 
ATCACAGGCG GGCTGCGACA GGAGGCCATC GATCGCTTTA ACGCACCCGG CGCTCAGCAG 
TTCTGTTTTC TGCTGTCCAC ACGTGCTGGA GGATTGGGTA TCAATCTGGC AACCGCAGAC 
ACAGTAATCA TATATGACTC TGACTGGAAT CCGCACAATG ACATTCAGGC ATTCAGCAGG 
GCGCATCGAA TTGGTCAAAA TAAAAAGGTG ATGATCTATC GCTTTGTAAC TCGGGCATCC 
GTAGAGGAGC GGATCACCCA GGTGGCCAAG CGAAAGATGA TGCTGACCCA CCTGGTGGTG 
CGGCCCGGCT TGGGCTCCAA GACGGGCTCT ATGTCCAAGC AAGAGCTAGA CGACATCCTC 
AAATTTGGCA CTGAAGAGCT TTTTAAAGAC GACGTGGAGG CCGCCAGAAC GATGGGTGAG 
CTGAAGGTGT TGGAGATGAA GAGTATTGTA CTTCCACTGT GCGTTCCAAT AGCTGAAGTC 
CTAAATCTGT CTTCAACAGG AGACAATAAA GAAGGAGAGG AGGTCAGTGT GATCCACTAT 
GACGATAATG CCATCTCCAA ACTTCTGGAC CGTAGTCAAG ATGCCACCGA AGACACTGAG 
ATCCAGAACA TGAACGAGTA CCTGAGCTCA TTTAAAGTGG CTCAGTATGT CGTGAGAGAA 
GAGGACGGAG AGGAGGAGGT CCAGAGGGAG ATTATTAAGC AGGAGGAGAA TGTTGATCCA 
GACTATTGGG AGAAGCTCTT GAGGCACCAT TATGAGCAGC AGCAGGAGGA TCTGGCTCGA 
AACCTGGGCA AGGGGAAACG CATCCGCAAA CAGGTCAACT ATAATGACGC CTCTCAAGAG 
GACCAAGAGT GGCAGGACGA TCTTTCGGAT AATCAGTCTG AGTACTCTGT CGGGTCTGAA 
GATGAAGATG AAGATTTTGA GGAACGGCCT GAGGGTGGAC GCAGACACTC CCGCAGACAG 
CTGAAGAGTG ATCGGGACAA ACCATTGCCG CCACTGCTGG CTAGAGTTAG TGGAAATATA 
GAGGTTTTGG GATTTAATGC CCGGCAGCGA AAAGCCTTCT TGAATGCGAT TATGCGCTGG 
GGAATGCCTC CGCAAGATGC CTTCAACTCA CACTGGCTCG TCCGTGACCT TAGAGGGAAA 
AGTGAGAAGG AGTTCAGGGC TTACGTGTCT CTGTTCATGA GGCACCTGTG TGAGCCGGGA 
GCAGATGGAG CAGAGACATT TGCAGATGGA GTCCCAAGGG AAGGCCTGTC CCGCCAGCAT 
GTTCTCACCC GGATCGGTGT CATGTCTCTG GTCCGCAAGA AGGTTCAGGA GTTTGAACAT 
GTGAATGGGA AGTTTAGTAC ACCAGATCGG ATTCCTGTAG GATTAGAGCT GAAAAAACTA 
ACAGAAAGTG TCTCGTCGGA TCCAAACACC CCTGTTCCTG CCAGTCCTGT TCCAACTCAA 
CCCAGCACAC CGGTTCCTTC AGATAAGACA GAGTCAGTGT CCGGGTCTCA AGAAGATAAG 
GAGATTGCAG AGCCAGAGAA CACCAAACCG CTGGACCCTG AGCCAGTTGT TCCAGAACCC 
TCTCCTGCGC CAGAAAAGAC CAATGAGGGA GAAGAAACAA AAAACGGCAG TCCTGAAGAG 
AAACGATTGG AGGAGAGCGA GAAAAGTGAC ACCCCTCCAG TCCAATCAGA GCCATCAGCC 
AATCAAGCAC CGCCACCTAC TAAGCAATGT GAGCAGCCAG CCAATCAGAC ACCTTCAGAC 
TGTGGTAAAG GAAAAGAGAC TTCTCCAGAA AGAGGGGAAA CTAAACCCAG CCCTCCAAAA 
ACAGAGGATA AGGAACAAAA ACCAGCCATT CCTGATGATG TAAAATCTGA AGACCCTCCT 
GAAAACCAAG TGAATGGAGA GAAAGATGTA AGAGATGAGA CGGACGAGGG CCACAGAGAT 
GACAAGAGCA GCATCAAAAC TCGCTTCATG TTCAACATTG CAGATGGAGG ATTCACAGAG 
TTGCACACGC TATGGCAGAA TGAGGAACGC GCAGCTGTGT CATCTGGCAA AATATACGAC 
ATCTGGCATC GTCGCCATGA CTACTGGCTG CTGGCTGGCA TCGTAACACA TGGTTATGCC 
CGCTGGCAGG ACATTCAGAA TGACCCACGT TATGCCATCC TCAATGAGCC TTTCAAGACT 
GAAATGCATA AAGGGAACTA CTTAGAAATG AAGAACAAGT TCCTTGCCAG ACGCTTTAAG 
CTACTGGAAC AAGCTTTGGT CATCGAGGAG CAGCTCCGGC GTGCGGCGTA CCTGAACATG 
ACTCAGGACC CCAGTCATCC TGCCATGGCC CTCAACACAC GTTTTGCAGA AGTGGAGTGT 
CTGGCCGAGT CTCATCAGCA CCTCTCCAAA GAGTCTCTGG CTGGTAACAA GCCAGCCAAT 
GCAGTGCTGC ACAAAGTGTT AAATCAGCTG GAGGAGTTGT TAAGTGATAT GAAGGCGGAT 
GTGACGCGTT TGCCCTCCAT GCTTTCGCGG GTGCCACCAG TGTCCGCGCG GCTCCAGATG 
TCAGAGCGGG GCATCCTCAG CAGACTCACC AGCCGGGGCA ATGAGCCGCC ACCTCAGCAG 
CCTTTCCCTC AGGGCTCTTT CGGCTGTTCT CAGATGTATA ATAGCAGTTT CGCCGGAGGG 
TTTCGTGGAC CTGGAGGCAC CGCCATGGTG AACTATAGCC AGATGCCCCT GGGGCCGTAC 
GTCAGTGTTT CCTCAAACGG GCCTCCTGCC CCTACCAGCC TCCTGGAGAA GAAGTCGAGT 
GACGTCCTGC GAGATGTGTG GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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japanmarket@genscript.com

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

chd5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報