ano3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ano3
Entrez Gene ID	553392
Full Name	anoctamin 3
Synonyms	tmem16c
General protein information	Preferred Names anoctamin 3 Names anoctamin-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470756.1	XP_021326431.1	anoctamin-3 isoform X2
XM_017354216.1	XP_017209705.1	anoctamin-3 isoform X1
XM_017354216.2	XP_017209705.1	anoctamin-3 isoform X3
XM_017354217.1	XP_017209706.1	anoctamin-3 isoform X2
XM_021470755.1	XP_021326430.1	anoctamin-3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANO3	NP_113606.2
Canis lupus familiaris (dog)	ANO3	XP_534094.3
Rattus norvegicus (Norway rat)	Ano3	XP_230381.7
Gallus gallus (chicken)	ANO3	NP_001186394.1
Pan troglodytes (chimpanzee)	ANO3	XP_001134720.1
Danio rerio (zebrafish)	ano3	XP_005163241.1
	ANO3	XP_001091004.1
Bos taurus (cattle)	ANO3	NP_001178244.2
Mus musculus (house mouse)	Ano3	NP_001121575.1
Xenopus tropicalis (tropical clawed frog)	ano3	XP_002936539.2

Gene Ontology

The following ano3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ano3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa57929	XM_017354216.2 Latest version!	Danio rerio anoctamin 3 (ano3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa57930	XM_017354217.1 Latest version!	Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa141503	XM_021470755.1 Latest version!	Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa141504	XM_021470756.1 Latest version!	Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ODa57929	XM_017354216.1	Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ano3 ( XM_017354216.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209705.1 Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa57929
Clone ID Related Accession (Same CDS sequence)	XM_017354216.1 , XM_017354216.2
Accession Version	XM_017354216.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCGCTGCT GCAGAACTGG 
TGGTCTCGGC GTAAGATGAA GCGAGCCGGA GAGCAGAGTA ACAGTAAAGA AGAGCTCCCG 
CAATGGGACA GAGACTGGAA CCTGCAGCCC ATGAACGCAC ACGGCCTGGT GGACGAGTAC 
CTGGAGATGG TTCTACAGTT TGGCTTCACT ACGATCTTCG TGGCGGCGTT TCCTCTGGCT 
CCCCTGCTGG CTCTGCTCAA CAACATCATC GAGATTCGAT TGGACGCCTA CAAGTTCGTT 
ACGCAGTGGA GGAGACCGAT GCCCGCCCGA GCTACCGACA TCGGTATCTG GCATGGTGTT 
CTGGAGGGAA TTGGAGTTGT GGCTGTCATA ACAAATGCCT TCGTCATCGC CATCACTTCA 
GATTACATCC CTCGCTTCGT CTACGCAATC AAATATGGGC CCTGTGTGGA CAAGGGTTAC 
CGACACGAGA AATGTCTAAG AGGATACCTG AACAACAGCC TGTCTGTGTT TGATATGGGT 
GAACTGAGAA ATGGGAGTTA TGAAAATCAA TACTGCAGGT ATCGGGATTA CAGAGCGCCT 
CCGTGGAGTC CTGAACCCTA TGAGTTCACC CTGCAGTTCT GGCATGTTTT AGCTGCCAGA 
CTAGCCTTCA TCATTGTCTT CGAGCACCTG GTGTTTGGCA TCAAGACCTT CATAGCCCAC 
ATGATTCCAG ACATGCCCAA AGACCTGTGT GATCGCATGC GCAGGGAGAA ATACCTGATG 
CAGGAGATGG TGTATGAGGC AGAACTGGAG CATCTCCAGA AAGAGCGGAA AAAGAACGGC 
AAACGTTATC ACCATGAGTG GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209705.1
CDS	194..3160
Translation	MSPAFIFCWTPGSLSGIIMVHHSGSIQSFKQQKGMHTSISEILKEKTLKSSRRSLPCLAQSQTHPENLNHFLSVPLSPGPKSYTHSSSVSASCTHITPPATDTGQDVYTEERETGGFEPKTDETLLVQQKPVSRGKLEARTDSVKGKNLETPSGLMFRDGKKRIDYILVYKKSSPQVEKRCTFERNLRAEGLMLEKEPSLTNSDIMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSWLPRNPMKLDKEALPDLVETDCYTAPFCRARMHHFTINNRETFFSNSTRSRIVHHVLQRTKYEDGKSKMGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCPMCEGNCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEEEELRPQFEAKYSRKERVNPISGKPEPFQPLTDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPLLQNWWSRRKMKRAGEQSNSKEELPQWDRDWNLQPMNAHGLVDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPMPARATDIGIWHGVLEGIGVVAVITNAFVIAITSDYIPRFVYAIKYGPCVDKGYRHEKCLRGYLNNSLSVFDMGELRNGSYENQYCRYRDYRAPPWSPEPYEFTLQFWHVLAARLAFIIVFEHLVFGIKTFIAHMIPDMPKDLCDRMRREKYLMQEMVYEAELEHLQKERKKNGKRYHHEWP

Target ORF information:

RefSeq Version	XM_017354216.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354216.1

ORF Insert Sequence:

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCGCTGCT GCAGAACTGG 
TGGTCTCGGC GTAAGATGAA GCGAGCCGGA GAGCAGAGTA ACAGTAAAGA AGAGCTCCCG 
CAATGGGACA GAGACTGGAA CCTGCAGCCC ATGAACGCAC ACGGCCTGGT GGACGAGTAC 
CTGGAGATGG TTCTACAGTT TGGCTTCACT ACGATCTTCG TGGCGGCGTT TCCTCTGGCT 
CCCCTGCTGG CTCTGCTCAA CAACATCATC GAGATTCGAT TGGACGCCTA CAAGTTCGTT 
ACGCAGTGGA GGAGACCGAT GCCCGCCCGA GCTACCGACA TCGGTATCTG GCATGGTGTT 
CTGGAGGGAA TTGGAGTTGT GGCTGTCATA ACAAATGCCT TCGTCATCGC CATCACTTCA 
GATTACATCC CTCGCTTCGT CTACGCAATC AAATATGGGC CCTGTGTGGA CAAGGGTTAC 
CGACACGAGA AATGTCTAAG AGGATACCTG AACAACAGCC TGTCTGTGTT TGATATGGGT 
GAACTGAGAA ATGGGAGTTA TGAAAATCAA TACTGCAGGT ATCGGGATTA CAGAGCGCCT 
CCGTGGAGTC CTGAACCCTA TGAGTTCACC CTGCAGTTCT GGCATGTTTT AGCTGCCAGA 
CTAGCCTTCA TCATTGTCTT CGAGCACCTG GTGTTTGGCA TCAAGACCTT CATAGCCCAC 
ATGATTCCAG ACATGCCCAA AGACCTGTGT GATCGCATGC GCAGGGAGAA ATACCTGATG 
CAGGAGATGG TGTATGAGGC AGAACTGGAG CATCTCCAGA AAGAGCGGAA AAAGAACGGC 
AAACGTTATC ACCATGAGTG GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano3 ( XM_017354216.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209705.1 Danio rerio anoctamin 3 (ano3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57929
Clone ID Related Accession (Same CDS sequence)	XM_017354216.1 , XM_017354216.2
Accession Version	XM_017354216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCGCTGCT GCAGAACTGG 
TGGTCTCGGC GTAAGATGAA GCGAGCCGGA GAGCAGAGTA ACAGTAAAGA AGAGCTCCCG 
CAATGGGACA GAGACTGGAA CCTGCAGCCC ATGAACGCAC ACGGCCTGGT GGACGAGTAC 
CTGGAGATGG TTCTACAGTT TGGCTTCACT ACGATCTTCG TGGCGGCGTT TCCTCTGGCT 
CCCCTGCTGG CTCTGCTCAA CAACATCATC GAGATTCGAT TGGACGCCTA CAAGTTCGTT 
ACGCAGTGGA GGAGACCGAT GCCCGCCCGA GCTACCGACA TCGGTATCTG GCATGGTGTT 
CTGGAGGGAA TTGGAGTTGT GGCTGTCATA ACAAATGCCT TCGTCATCGC CATCACTTCA 
GATTACATCC CTCGCTTCGT CTACGCAATC AAATATGGGC CCTGTGTGGA CAAGGGTTAC 
CGACACGAGA AATGTCTAAG AGGATACCTG AACAACAGCC TGTCTGTGTT TGATATGGGT 
GAACTGAGAA ATGGGAGTTA TGAAAATCAA TACTGCAGGT ATCGGGATTA CAGAGCGCCT 
CCGTGGAGTC CTGAACCCTA TGAGTTCACC CTGCAGTTCT GGCATGTTTT AGCTGCCAGA 
CTAGCCTTCA TCATTGTCTT CGAGCACCTG GTGTTTGGCA TCAAGACCTT CATAGCCCAC 
ATGATTCCAG ACATGCCCAA AGACCTGTGT GATCGCATGC GCAGGGAGAA ATACCTGATG 
CAGGAGATGG TGTATGAGGC AGAACTGGAG CATCTCCAGA AAGAGCGGAA AAAGAACGGC 
AAACGTTATC ACCATGAGTG GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209705.1
CDS	369..3335
Translation	MSPAFIFCWTPGSLSGIIMVHHSGSIQSFKQQKGMHTSISEILKEKTLKSSRRSLPCLAQSQTHPENLNHFLSVPLSPGPKSYTHSSSVSASCTHITPPATDTGQDVYTEERETGGFEPKTDETLLVQQKPVSRGKLEARTDSVKGKNLETPSGLMFRDGKKRIDYILVYKKSSPQVEKRCTFERNLRAEGLMLEKEPSLTNSDIMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSWLPRNPMKLDKEALPDLVETDCYTAPFCRARMHHFTINNRETFFSNSTRSRIVHHVLQRTKYEDGKSKMGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCPMCEGNCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEEEELRPQFEAKYSRKERVNPISGKPEPFQPLTDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPLLQNWWSRRKMKRAGEQSNSKEELPQWDRDWNLQPMNAHGLVDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPMPARATDIGIWHGVLEGIGVVAVITNAFVIAITSDYIPRFVYAIKYGPCVDKGYRHEKCLRGYLNNSLSVFDMGELRNGSYENQYCRYRDYRAPPWSPEPYEFTLQFWHVLAARLAFIIVFEHLVFGIKTFIAHMIPDMPKDLCDRMRREKYLMQEMVYEAELEHLQKERKKNGKRYHHEWP

Target ORF information:

RefSeq Version	XM_017354216.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 3 (ano3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354216.2

ORF Insert Sequence:

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCGCTGCT GCAGAACTGG 
TGGTCTCGGC GTAAGATGAA GCGAGCCGGA GAGCAGAGTA ACAGTAAAGA AGAGCTCCCG 
CAATGGGACA GAGACTGGAA CCTGCAGCCC ATGAACGCAC ACGGCCTGGT GGACGAGTAC 
CTGGAGATGG TTCTACAGTT TGGCTTCACT ACGATCTTCG TGGCGGCGTT TCCTCTGGCT 
CCCCTGCTGG CTCTGCTCAA CAACATCATC GAGATTCGAT TGGACGCCTA CAAGTTCGTT 
ACGCAGTGGA GGAGACCGAT GCCCGCCCGA GCTACCGACA TCGGTATCTG GCATGGTGTT 
CTGGAGGGAA TTGGAGTTGT GGCTGTCATA ACAAATGCCT TCGTCATCGC CATCACTTCA 
GATTACATCC CTCGCTTCGT CTACGCAATC AAATATGGGC CCTGTGTGGA CAAGGGTTAC 
CGACACGAGA AATGTCTAAG AGGATACCTG AACAACAGCC TGTCTGTGTT TGATATGGGT 
GAACTGAGAA ATGGGAGTTA TGAAAATCAA TACTGCAGGT ATCGGGATTA CAGAGCGCCT 
CCGTGGAGTC CTGAACCCTA TGAGTTCACC CTGCAGTTCT GGCATGTTTT AGCTGCCAGA 
CTAGCCTTCA TCATTGTCTT CGAGCACCTG GTGTTTGGCA TCAAGACCTT CATAGCCCAC 
ATGATTCCAG ACATGCCCAA AGACCTGTGT GATCGCATGC GCAGGGAGAA ATACCTGATG 
CAGGAGATGG TGTATGAGGC AGAACTGGAG CATCTCCAGA AAGAGCGGAA AAAGAACGGC 
AAACGTTATC ACCATGAGTG GCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano3 ( XM_017354217.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209706.1 Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa57930
Clone ID Related Accession (Same CDS sequence)	XM_017354217.1
Accession Version	XM_017354217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	anoctamin-3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCATGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209706.1
CDS	194..2344
Translation	MSPAFIFCWTPGSLSGIIMVHHSGSIQSFKQQKGMHTSISEILKEKTLKSSRRSLPCLAQSQTHPENLNHFLSVPLSPGPKSYTHSSSVSASCTHITPPATDTGQDVYTEERETGGFEPKTDETLLVQQKPVSRGKLEARTDSVKGKNLETPSGLMFRDGKKRIDYILVYKKSSPQVEKRCTFERNLRAEGLMLEKEPSLTNSDIMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSWLPRNPMKLDKEALPDLVETDCYTAPFCRARMHHFTINNRETFFSNSTRSRIVHHVLQRTKYEDGKSKMGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCPMCEGNCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEEEELRPQFEAKYSRKERVNPISGKPEPFQPLTDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPC

Target ORF information:

RefSeq Version	XM_017354217.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354217.1

ORF Insert Sequence:

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGG TATTAATCGT CTGCTGGGCA ACAGCACGTA TGAAGCCGCG 
TTTCCTCCAC ACGAGGGCTG TTACAAAAGC AGACACCCGA TTAAAACCCA CGGCGCTCAG 
AACCACAGAC ACCTTCTGTA CGAGAGATGG GCACGCTGGG GGATCTGGTA CAAATACCAG 
CCGCTCGACC TCATACGACG GTATTTCGGA GAGAAGATCG GTCTGTATTT CGCCTGGTTG 
GGCTGGTATA CGGGAATGCT AATCCCTGCT GCTTTAGTCG GCCTTTTCGT CTTCCTATAT 
GGGCTTTTCA CCATGGACTC CAGCCAAGTA AGTAAGGAGA TCTGTGAGGC GAACACCACC 
GTCATGTGCC CCATGTGTGA AGGCAACTGC AGCTCATGGA CACTCAGCGA CAGCTGCGTT 
TATGCAAAGG TGACCCATCT TTTTGATAAC GGAGGCACAG TTTTCTTCGC AATCTTCATG 
GCAATATGGG CCACGGTTTT CCTTGAGTTC TGGAAGAGGA GGAGAGCTGA ACTGACCTAC 
GACTGGGATT TGATCGACTG GGAGGAGGAA GAGGAGGAGC TGCGGCCCCA GTTTGAAGCC 
AAATACTCGC GGAAGGAGAG GGTAAACCCC ATCTCAGGGA AACCAGAGCC TTTCCAGCCC 
CTCACAGACA AGCTCAGCCG ACTCATGGTG TCTGTCTCCG GCATCTTCTT CATGATCTCT 
CTGGTTCTGA CGGCTGTGTT TGCTGTGGTG GTTTTCCGGC TCATTGCTAT GGAGAAGTTT 
GTCTCCTTCC AGTGGGAGTT TGTGAAGAAA AACTGGCAGT TCGCAACTTC AGGCACCGGA 
GTCTGCATCA ACTTCATGAT CATCATGTCC CTCAATGTGG TCTATGAGAA AGTGGCGTAT 
CTTCTGACTA ATCTGGAGCA TCCGCGCACC GAGTCGGAGT GGGAAAACAG CTTCGCCCTC 
AAGATGTTCC TCTTCCAGTT CGTCAATCTC AACAGCTCCA CTTTCTACAT CGCCTTCTTC 
TTAGGCAGGT TTGCTGGCAG GCCGGGTGAC TATAATAAGC TGTTTAACCG CTGGAGGATG 
GAGGAGTGTC ATCCCAGTGG GTGTCTGATC GACCTGTGTC TGCAAATGGG AGTGATCATG 
GTGCTGAAAC AGATCTGGAA CAACTTCATG GAGCTGGGCT ACCCATGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano3 ( XM_021470755.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326430.1 Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa141503
Clone ID Related Accession (Same CDS sequence)	XM_021470755.1
Accession Version	XM_021470755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGC TCCTCCGTTG TGTTGTCTGA ATCTGCTTTA TTATTATGGC 
CAGCACGAGC GGCTCCTCAT AGGCCAGTCA GGCAGCTCGT GGGAAGGTAT TAATCGTCTG 
CTGGGCAACA GCACGTATGA AGCCGCGTTT CCTCCACACG AGGGCTGTTA CAAAAGCAGA 
CACCCGATTA AAACCCACGG CGCTCAGAAC CACAGACACC TTCTGTACGA GAGATGGGCA 
CGCTGGGGGA TCTGGTACAA ATACCAGCCG CTCGACCTCA TACGACGGTA TTTCGGAGAG 
AAGATCGGTC TGTATTTCGC CTGGTTGGGC TGGTATACGG GAATGCTAAT CCCTGCTGCT 
TTAGTCGGCC TTTTCGTCTT CCTATATGGG CTTTTCACCA TGGACTCCAG CCAAGTAAGT 
AAGGAGATCT GTGAGGCGAA CACCACCGTC ATGTGCCCCA TGTGTGAAGG CAACTGCAGC 
TCATGGACAC TCAGCGACAG CTGCGTTTAT GCAAAGGTGA CCCATCTTTT TGATAACGGA 
GGCACAGTTT TCTTCGCAAT CTTCATGGCA ATATGGGCCA CGGTTTTCCT TGAGTTCTGG 
AAGAGGAGGA GAGCTGAACT GACCTACGAC TGGGATTTGA TCGACTGGGA GGAGGAAGAG 
TGTAAACCCT GTGTCATGGT GTGCTCTGAG GAGCTGCGGC CCCAGTTTGA AGCCAAATAC 
TCGCGGAAGG AGAGGGTAAA CCCCATCTCA GGGAAACCAG AGCCTTTCCA GCCCCTCACA 
GACAAGCTCA GCCGACTCAT GGTGTCTGTC TCCGGCATCT TCTTCATGAT CTCTCTGGTT 
CTGACGGCTG TGTTTGCTGT GGTGGTTTTC CGGCTCATTG CTATGGAGAA GTTTGTCTCC 
TTCCAGTGGG AGTTTGTGAA GAAAAACTGG CAGTTCGCAA CTTCAGGCAC CGGAGTCTGC 
ATCAACTTCA TGATCATCAT GTCCCTCAAT GTGGTCTATG AGAAAGTGGC GTATCTTCTG 
ACTAATCTGG AGCATCCGCG CACCGAGTCG GAGTGGGAAA ACAGCTTCGC CCTCAAGATG 
TTCCTCTTCC AGTTCGTCAA TCTCAACAGC TCCACTTTCT ACATCGCCTT CTTCTTAGGC 
AGGTTTGCTG GCAGGCCGGG TGACTATAAT AAGCTGTTTA ACCGCTGGAG GATGGAGGAG 
TGTCATCCCA GTGGGTGTCT GATCGACCTG TGTCTGCAAA TGGGAGTGAT CATGGTGCTG 
AAACAGATCT GGAACAACTT CATGGAGCTG GGCTACCCGC TGCTGCAGAA CTGGTGGTCT 
CGGCGTAAGA TGAAGCGAGC CGGAGAGCAG AGTAACAGTA AAGAAGAGCT CCCGCAATGG 
GACAGAGACT GGAACCTGCA GCCCATGAAC GCACACGGCC TGGTGGACGA GTACCTGGAG 
ATGGTTCTAC AGTTTGGCTT CACTACGATC TTCGTGGCGG CGTTTCCTCT GGCTCCCCTG 
CTGGCTCTGC TCAACAACAT CATCGAGATT CGATTGGACG CCTACAAGTT CGTTACGCAG 
TGGAGGAGAC CGATGCCCGC CCGAGCTACC GACATCGGTA TCTGGCATGG TGTTCTGGAG 
GGAATTGGAG TTGTGGCTGT CATAACAAAT GCCTTCGTCA TCGCCATCAC TTCAGATTAC 
ATCCCTCGCT TCGTCTACGC AATCAAATAT GGGCCCTGTG TGGACAAGGG TTACCGACAC 
GAGAAATGTC TAAGAGGATA CCTGAACAAC AGCCTGTCTG TGTTTGATAT GGGTGAACTG 
AGAAATGGGA GTTATGAAAA TCAATACTGC AGGTATCGGG ATTACAGAGC GCCTCCGTGG 
AGTCCTGAAC CCTATGAGTT CACCCTGCAG TTCTGGCATG TTTTAGCTGC CAGACTAGCC 
TTCATCATTG TCTTCGAGCA CCTGGTGTTT GGCATCAAGA CCTTCATAGC CCACATGATT 
CCAGACATGC CCAAAGACCT GTGTGATCGC ATGCGCAGGG AGAAATACCT GATGCAGGAG 
ATGGTGTATG AGGCAGAACT GGAGCATCTC CAGAAAGAGC GGAAAAAGAA CGGCAAACGT 
TATCACCATG AGTGGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326430.1
CDS	371..3451
Translation	MSPAFIFCWTPGSLSGIIMVHHSGSIQSFKQQKGMHTSISEILKEKTLKSSRRSLPCLAQSQTHPENLNHFLSVPLSPGPKSYTHSSSVSASCTHITPPATDTGQDVYTEERETGGFEPKTDETLLVQQKPVSRGKLEARTDSVKGKNLETPSGLMFRDGKKRIDYILVYKKSSPQVEKRCTFERNLRAEGLMLEKEPSLTNSDIMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSWLPRNPMKLDKEALPDLVETDCYTAPFCRARMHHFTINNRETFFSNSTRSRIVHHVLQRTKYEDGKSKMAPPLCCLNLLYYYGQHERLLIGQSGSSWEGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCPMCEGNCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEECKPCVMVCSEELRPQFEAKYSRKERVNPISGKPEPFQPLTDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPLLQNWWSRRKMKRAGEQSNSKEELPQWDRDWNLQPMNAHGLVDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPMPARATDIGIWHGVLEGIGVVAVITNAFVIAITSDYIPRFVYAIKYGPCVDKGYRHEKCLRGYLNNSLSVFDMGELRNGSYENQYCRYRDYRAPPWSPEPYEFTLQFWHVLAARLAFIIVFEHLVFGIKTFIAHMIPDMPKDLCDRMRREKYLMQEMVYEAELEHLQKERKKNGKRYHHEWP

Target ORF information:

RefSeq Version	XM_021470755.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 3 (ano3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470755.1

ORF Insert Sequence:

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGC TCCTCCGTTG TGTTGTCTGA ATCTGCTTTA TTATTATGGC 
CAGCACGAGC GGCTCCTCAT AGGCCAGTCA GGCAGCTCGT GGGAAGGTAT TAATCGTCTG 
CTGGGCAACA GCACGTATGA AGCCGCGTTT CCTCCACACG AGGGCTGTTA CAAAAGCAGA 
CACCCGATTA AAACCCACGG CGCTCAGAAC CACAGACACC TTCTGTACGA GAGATGGGCA 
CGCTGGGGGA TCTGGTACAA ATACCAGCCG CTCGACCTCA TACGACGGTA TTTCGGAGAG 
AAGATCGGTC TGTATTTCGC CTGGTTGGGC TGGTATACGG GAATGCTAAT CCCTGCTGCT 
TTAGTCGGCC TTTTCGTCTT CCTATATGGG CTTTTCACCA TGGACTCCAG CCAAGTAAGT 
AAGGAGATCT GTGAGGCGAA CACCACCGTC ATGTGCCCCA TGTGTGAAGG CAACTGCAGC 
TCATGGACAC TCAGCGACAG CTGCGTTTAT GCAAAGGTGA CCCATCTTTT TGATAACGGA 
GGCACAGTTT TCTTCGCAAT CTTCATGGCA ATATGGGCCA CGGTTTTCCT TGAGTTCTGG 
AAGAGGAGGA GAGCTGAACT GACCTACGAC TGGGATTTGA TCGACTGGGA GGAGGAAGAG 
TGTAAACCCT GTGTCATGGT GTGCTCTGAG GAGCTGCGGC CCCAGTTTGA AGCCAAATAC 
TCGCGGAAGG AGAGGGTAAA CCCCATCTCA GGGAAACCAG AGCCTTTCCA GCCCCTCACA 
GACAAGCTCA GCCGACTCAT GGTGTCTGTC TCCGGCATCT TCTTCATGAT CTCTCTGGTT 
CTGACGGCTG TGTTTGCTGT GGTGGTTTTC CGGCTCATTG CTATGGAGAA GTTTGTCTCC 
TTCCAGTGGG AGTTTGTGAA GAAAAACTGG CAGTTCGCAA CTTCAGGCAC CGGAGTCTGC 
ATCAACTTCA TGATCATCAT GTCCCTCAAT GTGGTCTATG AGAAAGTGGC GTATCTTCTG 
ACTAATCTGG AGCATCCGCG CACCGAGTCG GAGTGGGAAA ACAGCTTCGC CCTCAAGATG 
TTCCTCTTCC AGTTCGTCAA TCTCAACAGC TCCACTTTCT ACATCGCCTT CTTCTTAGGC 
AGGTTTGCTG GCAGGCCGGG TGACTATAAT AAGCTGTTTA ACCGCTGGAG GATGGAGGAG 
TGTCATCCCA GTGGGTGTCT GATCGACCTG TGTCTGCAAA TGGGAGTGAT CATGGTGCTG 
AAACAGATCT GGAACAACTT CATGGAGCTG GGCTACCCGC TGCTGCAGAA CTGGTGGTCT 
CGGCGTAAGA TGAAGCGAGC CGGAGAGCAG AGTAACAGTA AAGAAGAGCT CCCGCAATGG 
GACAGAGACT GGAACCTGCA GCCCATGAAC GCACACGGCC TGGTGGACGA GTACCTGGAG 
ATGGTTCTAC AGTTTGGCTT CACTACGATC TTCGTGGCGG CGTTTCCTCT GGCTCCCCTG 
CTGGCTCTGC TCAACAACAT CATCGAGATT CGATTGGACG CCTACAAGTT CGTTACGCAG 
TGGAGGAGAC CGATGCCCGC CCGAGCTACC GACATCGGTA TCTGGCATGG TGTTCTGGAG 
GGAATTGGAG TTGTGGCTGT CATAACAAAT GCCTTCGTCA TCGCCATCAC TTCAGATTAC 
ATCCCTCGCT TCGTCTACGC AATCAAATAT GGGCCCTGTG TGGACAAGGG TTACCGACAC 
GAGAAATGTC TAAGAGGATA CCTGAACAAC AGCCTGTCTG TGTTTGATAT GGGTGAACTG 
AGAAATGGGA GTTATGAAAA TCAATACTGC AGGTATCGGG ATTACAGAGC GCCTCCGTGG 
AGTCCTGAAC CCTATGAGTT CACCCTGCAG TTCTGGCATG TTTTAGCTGC CAGACTAGCC 
TTCATCATTG TCTTCGAGCA CCTGGTGTTT GGCATCAAGA CCTTCATAGC CCACATGATT 
CCAGACATGC CCAAAGACCT GTGTGATCGC ATGCGCAGGG AGAAATACCT GATGCAGGAG 
ATGGTGTATG AGGCAGAACT GGAGCATCTC CAGAAAGAGC GGAAAAAGAA CGGCAAACGT 
TATCACCATG AGTGGCCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ano3 ( XM_021470756.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326431.1 Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.

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CloneID	ODa141504
Clone ID Related Accession (Same CDS sequence)	XM_021470756.1
Accession Version	XM_021470756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	anoctamin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGC TCCTCCGTTG TGTTGTCTGA ATCTGCTTTA TTATTATGGC 
CAGCACGAGC GGCTCCTCAT AGGCCAGTCA GGCAGCTCGT GGGAAGGTAT TAATCGTCTG 
CTGGGCAACA GCACGTATGA AGCCGCGTTT CCTCCACACG AGGGCTGTTA CAAAAGCAGA 
CACCCGATTA AAACCCACGG CGCTCAGAAC CACAGACACC TTCTGTACGA GAGATGGGCA 
CGCTGGGGGA TCTGGTACAA ATACCAGCCG CTCGACCTCA TACGACGGTA TTTCGGAGAG 
AAGATCGGTC TGTATTTCGC CTGGTTGGGC TGGTATACGG GAATGCTAAT CCCTGCTGCT 
TTAGTCGGCC TTTTCGTCTT CCTATATGGG CTTTTCACCA TGGACTCCAG CCAAGTAAGT 
AAGGAGATCT GTGAGGCGAA CACCACCGTC ATGTGCCCCA TGTGTGAAGG CAACTGCAGC 
TCATGGACAC TCAGCGACAG CTGCGTTTAT GCAAAGGTGA CCCATCTTTT TGATAACGGA 
GGCACAGTTT TCTTCGCAAT CTTCATGGCA ATATGGGCCA CGGTTTTCCT TGAGTTCTGG 
AAGAGGAGGA GAGCTGAACT GACCTACGAC TGGGATTTGA TCGACTGGGA GGAGGAAGAG 
GAGGAGCTGC GGCCCCAGTT TGAAGCCAAA TACTCGCGGA AGGAGAGGGT AAACCCCATC 
TCAGGGAAAC CAGAGCCTTT CCAGCCCCTC ACAGACAAGC TCAGCCGACT CATGGTGTCT 
GTCTCCGGCA TCTTCTTCAT GATCTCTCTG GTTCTGACGG CTGTGTTTGC TGTGGTGGTT 
TTCCGGCTCA TTGCTATGGA GAAGTTTGTC TCCTTCCAGT GGGAGTTTGT GAAGAAAAAC 
TGGCAGTTCG CAACTTCAGG CACCGGAGTC TGCATCAACT TCATGATCAT CATGTCCCTC 
AATGTGGTCT ATGAGAAAGT GGCGTATCTT CTGACTAATC TGGAGCATCC GCGCACCGAG 
TCGGAGTGGG AAAACAGCTT CGCCCTCAAG ATGTTCCTCT TCCAGTTCGT CAATCTCAAC 
AGCTCCACTT TCTACATCGC CTTCTTCTTA GGCAGGTTTG CTGGCAGGCC GGGTGACTAT 
AATAAGCTGT TTAACCGCTG GAGGATGGAG GAGTGTCATC CCAGTGGGTG TCTGATCGAC 
CTGTGTCTGC AAATGGGAGT GATCATGGTG CTGAAACAGA TCTGGAACAA CTTCATGGAG 
CTGGGCTACC CGCTGCTGCA GAACTGGTGG TCTCGGCGTA AGATGAAGCG AGCCGGAGAG 
CAGAGTAACA GTAAAGAAGA GCTCCCGCAA TGGGACAGAG ACTGGAACCT GCAGCCCATG 
AACGCACACG GCCTGGTGGA CGAGTACCTG GAGATGGTTC TACAGTTTGG CTTCACTACG 
ATCTTCGTGG CGGCGTTTCC TCTGGCTCCC CTGCTGGCTC TGCTCAACAA CATCATCGAG 
ATTCGATTGG ACGCCTACAA GTTCGTTACG CAGTGGAGGA GACCGATGCC CGCCCGAGCT 
ACCGACATCG GTATCTGGCA TGGTGTTCTG GAGGGAATTG GAGTTGTGGC TGTCATAACA 
AATGCCTTCG TCATCGCCAT CACTTCAGAT TACATCCCTC GCTTCGTCTA CGCAATCAAA 
TATGGGCCCT GTGTGGACAA GGGTTACCGA CACGAGAAAT GTCTAAGAGG ATACCTGAAC 
AACAGCCTGT CTGTGTTTGA TATGGGTGAA CTGAGAAATG GGAGTTATGA AAATCAATAC 
TGCAGGTATC GGGATTACAG AGCGCCTCCG TGGAGTCCTG AACCCTATGA GTTCACCCTG 
CAGTTCTGGC ATGTTTTAGC TGCCAGACTA GCCTTCATCA TTGTCTTCGA GCACCTGGTG 
TTTGGCATCA AGACCTTCAT AGCCCACATG ATTCCAGACA TGCCCAAAGA CCTGTGTGAT 
CGCATGCGCA GGGAGAAATA CCTGATGCAG GAGATGGTGT ATGAGGCAGA ACTGGAGCAT 
CTCCAGAAAG AGCGGAAAAA GAACGGCAAA CGTTATCACC ATGAGTGGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326431.1
CDS	370..3423
Translation	MSPAFIFCWTPGSLSGIIMVHHSGSIQSFKQQKGMHTSISEILKEKTLKSSRRSLPCLAQSQTHPENLNHFLSVPLSPGPKSYTHSSSVSASCTHITPPATDTGQDVYTEERETGGFEPKTDETLLVQQKPVSRGKLEARTDSVKGKNLETPSGLMFRDGKKRIDYILVYKKSSPQVEKRCTFERNLRAEGLMLEKEPSLTNSDIMFVKIHAPWDTLCKYAEQMNIRMPFRKKCYFTDWKKKAMGSRFQLRFRQLKSWLPRNPMKLDKEALPDLVETDCYTAPFCRARMHHFTINNRETFFSNSTRSRIVHHVLQRTKYEDGKSKMAPPLCCLNLLYYYGQHERLLIGQSGSSWEGINRLLGNSTYEAAFPPHEGCYKSRHPIKTHGAQNHRHLLYERWARWGIWYKYQPLDLIRRYFGEKIGLYFAWLGWYTGMLIPAALVGLFVFLYGLFTMDSSQVSKEICEANTTVMCPMCEGNCSSWTLSDSCVYAKVTHLFDNGGTVFFAIFMAIWATVFLEFWKRRRAELTYDWDLIDWEEEEEELRPQFEAKYSRKERVNPISGKPEPFQPLTDKLSRLMVSVSGIFFMISLVLTAVFAVVVFRLIAMEKFVSFQWEFVKKNWQFATSGTGVCINFMIIMSLNVVYEKVAYLLTNLEHPRTESEWENSFALKMFLFQFVNLNSSTFYIAFFLGRFAGRPGDYNKLFNRWRMEECHPSGCLIDLCLQMGVIMVLKQIWNNFMELGYPLLQNWWSRRKMKRAGEQSNSKEELPQWDRDWNLQPMNAHGLVDEYLEMVLQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPMPARATDIGIWHGVLEGIGVVAVITNAFVIAITSDYIPRFVYAIKYGPCVDKGYRHEKCLRGYLNNSLSVFDMGELRNGSYENQYCRYRDYRAPPWSPEPYEFTLQFWHVLAARLAFIIVFEHLVFGIKTFIAHMIPDMPKDLCDRMRREKYLMQEMVYEAELEHLQKERKKNGKRYHHEWP

Target ORF information:

RefSeq Version	XM_021470756.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio anoctamin 3 (ano3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470756.1

ORF Insert Sequence:

ATGTCTCCTG CCTTCATCTT CTGCTGGACG CCTGGATCTC TGTCGGGAAT CATCATGGTG 
CATCACTCCG GGTCCATACA GTCCTTCAAG CAGCAGAAGG GAATGCACAC CAGCATCAGT 
GAGATCTTGA AGGAGAAAAC GCTCAAGTCG TCTCGCCGCA GTTTGCCGTG TTTGGCTCAG 
AGTCAAACGC ATCCTGAAAA CCTCAACCAC TTCCTGTCTG TGCCTCTGAG TCCTGGACCC 
AAATCATACA CACACAGCAG CAGCGTCAGT GCCTCCTGCA CACACATCAC ACCTCCGGCC 
ACAGACACAG GTCAGGATGT CTACACTGAA GAGAGGGAGA CGGGGGGGTT TGAGCCGAAA 
ACAGATGAAA CTCTCCTCGT CCAGCAGAAG CCAGTGAGCA GAGGGAAACT AGAGGCCCGA 
ACTGACTCCG TAAAGGGGAA AAATCTAGAA ACCCCCTCGG GTCTGATGTT CAGAGATGGA 
AAAAAGCGTA TCGACTATAT CCTGGTGTAT AAGAAGTCGA GTCCGCAGGT GGAGAAGAGA 
TGCACGTTTG AGAGGAACCT GCGGGCTGAA GGACTAATGC TGGAGAAGGA GCCCTCATTA 
ACCAACAGTG ATATCATGTT TGTGAAGATT CATGCGCCGT GGGACACGCT GTGTAAATAC 
GCCGAGCAGA TGAACATCAG GATGCCCTTT AGGAAAAAGT GTTACTTCAC TGACTGGAAG 
AAGAAAGCTA TGGGCAGTCG ATTTCAGCTG AGGTTTCGCC AGCTGAAGAG TTGGCTGCCC 
AGGAACCCGA TGAAGCTGGA TAAAGAGGCT CTTCCAGACC TGGTGGAGAC GGACTGTTAT 
ACGGCACCCT TCTGCAGAGC CAGAATGCAC CATTTCACCA TCAACAACAG AGAGACGTTT 
TTCTCCAACT CCACCAGAAG CAGAATCGTT CATCATGTGC TTCAGCGGAC AAAGTACGAG 
GACGGAAAGT CAAAGATGGC TCCTCCGTTG TGTTGTCTGA ATCTGCTTTA TTATTATGGC 
CAGCACGAGC GGCTCCTCAT AGGCCAGTCA GGCAGCTCGT GGGAAGGTAT TAATCGTCTG 
CTGGGCAACA GCACGTATGA AGCCGCGTTT CCTCCACACG AGGGCTGTTA CAAAAGCAGA 
CACCCGATTA AAACCCACGG CGCTCAGAAC CACAGACACC TTCTGTACGA GAGATGGGCA 
CGCTGGGGGA TCTGGTACAA ATACCAGCCG CTCGACCTCA TACGACGGTA TTTCGGAGAG 
AAGATCGGTC TGTATTTCGC CTGGTTGGGC TGGTATACGG GAATGCTAAT CCCTGCTGCT 
TTAGTCGGCC TTTTCGTCTT CCTATATGGG CTTTTCACCA TGGACTCCAG CCAAGTAAGT 
AAGGAGATCT GTGAGGCGAA CACCACCGTC ATGTGCCCCA TGTGTGAAGG CAACTGCAGC 
TCATGGACAC TCAGCGACAG CTGCGTTTAT GCAAAGGTGA CCCATCTTTT TGATAACGGA 
GGCACAGTTT TCTTCGCAAT CTTCATGGCA ATATGGGCCA CGGTTTTCCT TGAGTTCTGG 
AAGAGGAGGA GAGCTGAACT GACCTACGAC TGGGATTTGA TCGACTGGGA GGAGGAAGAG 
GAGGAGCTGC GGCCCCAGTT TGAAGCCAAA TACTCGCGGA AGGAGAGGGT AAACCCCATC 
TCAGGGAAAC CAGAGCCTTT CCAGCCCCTC ACAGACAAGC TCAGCCGACT CATGGTGTCT 
GTCTCCGGCA TCTTCTTCAT GATCTCTCTG GTTCTGACGG CTGTGTTTGC TGTGGTGGTT 
TTCCGGCTCA TTGCTATGGA GAAGTTTGTC TCCTTCCAGT GGGAGTTTGT GAAGAAAAAC 
TGGCAGTTCG CAACTTCAGG CACCGGAGTC TGCATCAACT TCATGATCAT CATGTCCCTC 
AATGTGGTCT ATGAGAAAGT GGCGTATCTT CTGACTAATC TGGAGCATCC GCGCACCGAG 
TCGGAGTGGG AAAACAGCTT CGCCCTCAAG ATGTTCCTCT TCCAGTTCGT CAATCTCAAC 
AGCTCCACTT TCTACATCGC CTTCTTCTTA GGCAGGTTTG CTGGCAGGCC GGGTGACTAT 
AATAAGCTGT TTAACCGCTG GAGGATGGAG GAGTGTCATC CCAGTGGGTG TCTGATCGAC 
CTGTGTCTGC AAATGGGAGT GATCATGGTG CTGAAACAGA TCTGGAACAA CTTCATGGAG 
CTGGGCTACC CGCTGCTGCA GAACTGGTGG TCTCGGCGTA AGATGAAGCG AGCCGGAGAG 
CAGAGTAACA GTAAAGAAGA GCTCCCGCAA TGGGACAGAG ACTGGAACCT GCAGCCCATG 
AACGCACACG GCCTGGTGGA CGAGTACCTG GAGATGGTTC TACAGTTTGG CTTCACTACG 
ATCTTCGTGG CGGCGTTTCC TCTGGCTCCC CTGCTGGCTC TGCTCAACAA CATCATCGAG 
ATTCGATTGG ACGCCTACAA GTTCGTTACG CAGTGGAGGA GACCGATGCC CGCCCGAGCT 
ACCGACATCG GTATCTGGCA TGGTGTTCTG GAGGGAATTG GAGTTGTGGC TGTCATAACA 
AATGCCTTCG TCATCGCCAT CACTTCAGAT TACATCCCTC GCTTCGTCTA CGCAATCAAA 
TATGGGCCCT GTGTGGACAA GGGTTACCGA CACGAGAAAT GTCTAAGAGG ATACCTGAAC 
AACAGCCTGT CTGTGTTTGA TATGGGTGAA CTGAGAAATG GGAGTTATGA AAATCAATAC 
TGCAGGTATC GGGATTACAG AGCGCCTCCG TGGAGTCCTG AACCCTATGA GTTCACCCTG 
CAGTTCTGGC ATGTTTTAGC TGCCAGACTA GCCTTCATCA TTGTCTTCGA GCACCTGGTG 
TTTGGCATCA AGACCTTCAT AGCCCACATG ATTCCAGACA TGCCCAAAGA CCTGTGTGAT 
CGCATGCGCA GGGAGAAATA CCTGATGCAG GAGATGGTGT ATGAGGCAGA ACTGGAGCAT 
CTCCAGAAAG AGCGGAAAAA GAACGGCAAA CGTTATCACC ATGAGTGGCC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ano3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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