p3h1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	p3h1
Entrez Gene ID	553321
Full Name	prolyl 3-hydroxylase 1
Synonyms	lepre1,sb:cb953
General protein information	Preferred Names prolyl 3-hydroxylase 1 Names prolyl 3-hydroxylase 1 leucine proline-enriched proteoglycan (leprecan) 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002663819.4	XP_002663865.1	prolyl 3-hydroxylase 1
XM_002663819.5	XP_002663865.1	prolyl 3-hydroxylase 1

Pathways from BioSystems

Homologies

	LEPRE1	XP_001088904.1
Mus musculus (house mouse)	Lepre1	NP_062757.2
Homo sapiens (human)	LEPRE1	NP_071751.3
Rattus norvegicus (Norway rat)	Lepre1	NP_446119.1
Gallus gallus (chicken)	LEPRE1	NP_001001529.1
Danio rerio (zebrafish)	lepre1	XP_002663865.1
Xenopus tropicalis (tropical clawed frog)	lepre1	NP_001090700.1
Pan troglodytes (chimpanzee)	LEPRE1	XP_003308086.1
Canis lupus familiaris (dog)	LEPRE1	XP_848570.2
Bos taurus (cattle)	LEPRE1	NP_001096761.1

Gene Ontology

The following p3h1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the p3h1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa17329	XM_002663819.5 Latest version!	Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa17329	XM_002663819.4	Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

p3h1 ( XM_002663819.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663865.1 Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.

Price & Availability↑

CloneID	ODa17329
Clone ID Related Accession (Same CDS sequence)	XM_002663819.4 , XM_002663819.5
Accession Version	XM_002663819.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	prolyl 3-hydroxylase 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACCT CACTGTGCCT CATCTCAATC CTCTCCACTG TCCACTTCAC CCGGTGTGAC 
CCTCAAATCA ATGCGGGATT TCAGCCGTAC GACCTGCTCT TCGACAACGC GGTGGAGGCG 
TATTATAAGC AGGACTGGCT GGCTGTGATC CTCAACATGG AGAAAGCGCT GCGGAATAAA 
GGAGCGCTGC GTAAAATACA GACGCACTGT CGCCTGTCCT GCGCCAATAA CACCGCTTTT 
GGGGAGCCTC TTCCCGGTTT GGGCATCCCG ATCCCAGGCT CCGGCTCTGT GGAGGATTTA 
GCGTTTTTCC AGAGGATCCT CCAACGGGCC GATTGCGTGA ATGCGTGTGA AAGCGAGAAA 
CTGGGACCGG CGACCCTGCA TAAAGTTAGT GAGAATGTTG AGCTGGAGTT TAAGAAGAGG 
ACGCCGTATA ATTACCTGCA AGTGGCCTAC TTTAAGATAA ACAAGCTTGA CAAGGCAGTG 
GCAGCAGCAA ATACATTTTT CATGGCGAAC CCCGAGCATG TGGAGATGAA ACAGAATTTA 
GATTATTACA GGATGATGGC TGGGGTGCAG GAGACCGATT TCAAGGACCT GGAGGAGAAA 
CCGCACATGG CAGAATTTTT GCAAGGCAAG ATTCATTACA GTACGGAAGA TTTCGTTCCA 
GCCGTCGAGC ATTTTGAGGC GGCGGTTGAA GAGTATTTCG GCGCTTATGA GGAATGCCGA 
GTTCTGTGTG AGGGAGCGTT CGATTATGAT GGATACAACT ACATGGAGTA CAACGCTGAT 
CTCTTCCAGT CAATGACAGA TCATTATCTT CATGTTTTGA GCTGCAAACA AAACTGTGCC 
GTTGAGCTGG CATCCACCGC TGGGAGAGAA ACACCTTTTG AAGATTTCCT GCCCTCACAC 
TTCAACTACC TGCAGTTCTC ATACTATAAC ATGGAGAAAT TCGAGCAGGC CATTGAGTGT 
GCTAAAACAT ACCTGCTCTT CCACCCTGAG GATGCAGTCA TGGCTCAGAA CATTGCGTAT 
TACTCCGCTG TGCTGGGAGA CGAAAAAGCC GTCAACATCA CGGAAAGAGA GGTGGTTAAA 
CAGCATATAA AGAGATCTCT GCTGGAAAAA GAGCTCCTTT ACTACGGCTA TGAGATGTTT 
GGTAAAGCGT TTGTTGATCC AGACACATGG ACACCTGAGG AAGTCATGCC CAAGAAACTA 
AGAGACAAAC AGAAGGTGGA CAAAGAAACA GCGGCGAGGA TTACTGAGGA GATCGGTAAC 
CTGATGAAAG AGATAGAGAC ACTGGTGGAA GAAAAGAGTA AAGAGTCATC AGACATCGTC 
AAGGTTGTGA AAGATGAAAT CAAACCAGCT GCTGCTCCAG ATACTGCCAG CAAACTCCCA 
TCTGATGACA TCACCATAAC CATGACCTCC GAGGTTCTGA GCGGTTCACA TCGAGTTGTC 
CTAGATGGAG TAACCACATC TGATGAGTGT CAGGAACTTC ATCAAATCAC CACTACAGCA 
GCTCTTCAAG ATGCTTTTAA AGCAGTGCCA TCCCCTCATT CAGCGAGTGA GATGTTTCAG 
GACGTCCCAG TTCTCAAAGC TCTGCAGGAA GGTCTAATTC CTACAAAAAG TGCACATTTG 
CTGTCCAATC TAGGTGAGAA GGTCCGTAAG GTTCTTGAAT CCTACTTCGC TCTGGAATCA 
ACGCTCTATT TCTCCAGCTC AAACCTGGTC TGCAGATCTG CCACTGAAAA ACAGGAGGCC 
AGTGCAGACT GTCTGCTTAT TTCCGAGCTC AACGAATGTA TCAAAGTGCT GTCAACAAAC 
ACCAATCAAG GCTACAGTGC CATTTTGTAC CTCAATGATG ATTTCGAAGG AGGAGATTTT 
ATCTTCACCG AGTCGGATGG CAAAGCTGTC TCTGCCGCAG TGAAGCCTCA GTGTGGTCGA 
GTGGTGGGCT TCAGAGCCGG ACAGGAGAAT CTTCATGGGG TCTCAGCGGT AACTAAAGGC 
CAGAGGTGTG CTGTAGTGCT TCACTTCACT CTGGATCCTC AGCACAGTCA GGAGGAGCGT 
TCACAGACTG AAGAACTTCT GAAAAAGCTT TCGAGCCCAG TTAAAGTAGA CATCCAGAAA 
ACGGCGACAG ACAGTCAAGA GTACCCCTCC AAACCCGAAG GAACAATTCC TGCCGAAACA 
ACAGAGAAAG ACAAAAGCAA AGCGACTGAT GACAACACAG CTGAGACAAA AACAGACAAT 
AAAGCTAGCA AGAAAAGCGA AAAACCAAAG GCCAAAACAA CAAAAAAGAC ATCAAAAGCA 
GAAACCCAAA CAAAAGCTAA AGCAGACAAA ACAAAAACCA CAACAGCGGA GAAAAAGAAA 
ACAAAACCAG CCAGCAAGAC AAAACCTGTA ACGACAGACA AAAAGGAGAA ACCGCCCGGC 
AAGAAAACAG CTGCAAATGA CAAGAAAACC AAACCAAAAG CCACAGAAGC GAAAGACTCC 
AAAGCTGAAA AACACAACGA TGAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663865.1
CDS	58..2547
Translation	MWTSLCLISILSTVHFTRCDPQINAGFQPYDLLFDNAVEAYYKQDWLAVILNMEKALRNKGALRKIQTHCRLSCANNTAFGEPLPGLGIPIPGSGSVEDLAFFQRILQRADCVNACESEKLGPATLHKVSENVELEFKKRTPYNYLQVAYFKINKLDKAVAAANTFFMANPEHVEMKQNLDYYRMMAGVQETDFKDLEEKPHMAEFLQGKIHYSTEDFVPAVEHFEAAVEEYFGAYEECRVLCEGAFDYDGYNYMEYNADLFQSMTDHYLHVLSCKQNCAVELASTAGRETPFEDFLPSHFNYLQFSYYNMEKFEQAIECAKTYLLFHPEDAVMAQNIAYYSAVLGDEKAVNITEREVVKQHIKRSLLEKELLYYGYEMFGKAFVDPDTWTPEEVMPKKLRDKQKVDKETAARITEEIGNLMKEIETLVEEKSKESSDIVKVVKDEIKPAAAPDTASKLPSDDITITMTSEVLSGSHRVVLDGVTTSDECQELHQITTTAALQDAFKAVPSPHSASEMFQDVPVLKALQEGLIPTKSAHLLSNLGEKVRKVLESYFALESTLYFSSSNLVCRSATEKQEASADCLLISELNECIKVLSTNTNQGYSAILYLNDDFEGGDFIFTESDGKAVSAAVKPQCGRVVGFRAGQENLHGVSAVTKGQRCAVVLHFTLDPQHSQEERSQTEELLKKLSSPVKVDIQKTATDSQEYPSKPEGTIPAETTEKDKSKATDDNTAETKTDNKASKKSEKPKAKTTKKTSKAETQTKAKADKTKTTTAEKKKTKPASKTKPVTTDKKEKPPGKKTAANDKKTKPKATEAKDSKAEKHNDEL

Target ORF information:

RefSeq Version	XM_002663819.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663819.4

ORF Insert Sequence:

ATGTGGACCT CACTGTGCCT CATCTCAATC CTCTCCACTG TCCACTTCAC CCGGTGTGAC 
CCTCAAATCA ATGCGGGATT TCAGCCGTAC GACCTGCTCT TCGACAACGC GGTGGAGGCG 
TATTATAAGC AGGACTGGCT GGCTGTGATC CTCAACATGG AGAAAGCGCT GCGGAATAAA 
GGAGCGCTGC GTAAAATACA GACGCACTGT CGCCTGTCCT GCGCCAATAA CACCGCTTTT 
GGGGAGCCTC TTCCCGGTTT GGGCATCCCG ATCCCAGGCT CCGGCTCTGT GGAGGATTTA 
GCGTTTTTCC AGAGGATCCT CCAACGGGCC GATTGCGTGA ATGCGTGTGA AAGCGAGAAA 
CTGGGACCGG CGACCCTGCA TAAAGTTAGT GAGAATGTTG AGCTGGAGTT TAAGAAGAGG 
ACGCCGTATA ATTACCTGCA AGTGGCCTAC TTTAAGATAA ACAAGCTTGA CAAGGCAGTG 
GCAGCAGCAA ATACATTTTT CATGGCGAAC CCCGAGCATG TGGAGATGAA ACAGAATTTA 
GATTATTACA GGATGATGGC TGGGGTGCAG GAGACCGATT TCAAGGACCT GGAGGAGAAA 
CCGCACATGG CAGAATTTTT GCAAGGCAAG ATTCATTACA GTACGGAAGA TTTCGTTCCA 
GCCGTCGAGC ATTTTGAGGC GGCGGTTGAA GAGTATTTCG GCGCTTATGA GGAATGCCGA 
GTTCTGTGTG AGGGAGCGTT CGATTATGAT GGATACAACT ACATGGAGTA CAACGCTGAT 
CTCTTCCAGT CAATGACAGA TCATTATCTT CATGTTTTGA GCTGCAAACA AAACTGTGCC 
GTTGAGCTGG CATCCACCGC TGGGAGAGAA ACACCTTTTG AAGATTTCCT GCCCTCACAC 
TTCAACTACC TGCAGTTCTC ATACTATAAC ATGGAGAAAT TCGAGCAGGC CATTGAGTGT 
GCTAAAACAT ACCTGCTCTT CCACCCTGAG GATGCAGTCA TGGCTCAGAA CATTGCGTAT 
TACTCCGCTG TGCTGGGAGA CGAAAAAGCC GTCAACATCA CGGAAAGAGA GGTGGTTAAA 
CAGCATATAA AGAGATCTCT GCTGGAAAAA GAGCTCCTTT ACTACGGCTA TGAGATGTTT 
GGTAAAGCGT TTGTTGATCC AGACACATGG ACACCTGAGG AAGTCATGCC CAAGAAACTA 
AGAGACAAAC AGAAGGTGGA CAAAGAAACA GCGGCGAGGA TTACTGAGGA GATCGGTAAC 
CTGATGAAAG AGATAGAGAC ACTGGTGGAA GAAAAGAGTA AAGAGTCATC AGACATCGTC 
AAGGTTGTGA AAGATGAAAT CAAACCAGCT GCTGCTCCAG ATACTGCCAG CAAACTCCCA 
TCTGATGACA TCACCATAAC CATGACCTCC GAGGTTCTGA GCGGTTCACA TCGAGTTGTC 
CTAGATGGAG TAACCACATC TGATGAGTGT CAGGAACTTC ATCAAATCAC CACTACAGCA 
GCTCTTCAAG ATGCTTTTAA AGCAGTGCCA TCCCCTCATT CAGCGAGTGA GATGTTTCAG 
GACGTCCCAG TTCTCAAAGC TCTGCAGGAA GGTCTAATTC CTACAAAAAG TGCACATTTG 
CTGTCCAATC TAGGTGAGAA GGTCCGTAAG GTTCTTGAAT CCTACTTCGC TCTGGAATCA 
ACGCTCTATT TCTCCAGCTC AAACCTGGTC TGCAGATCTG CCACTGAAAA ACAGGAGGCC 
AGTGCAGACT GTCTGCTTAT TTCCGAGCTC AACGAATGTA TCAAAGTGCT GTCAACAAAC 
ACCAATCAAG GCTACAGTGC CATTTTGTAC CTCAATGATG ATTTCGAAGG AGGAGATTTT 
ATCTTCACCG AGTCGGATGG CAAAGCTGTC TCTGCCGCAG TGAAGCCTCA GTGTGGTCGA 
GTGGTGGGCT TCAGAGCCGG ACAGGAGAAT CTTCATGGGG TCTCAGCGGT AACTAAAGGC 
CAGAGGTGTG CTGTAGTGCT TCACTTCACT CTGGATCCTC AGCACAGTCA GGAGGAGCGT 
TCACAGACTG AAGAACTTCT GAAAAAGCTT TCGAGCCCAG TTAAAGTAGA CATCCAGAAA 
ACGGCGACAG ACAGTCAAGA GTACCCCTCC AAACCCGAAG GAACAATTCC TGCCGAAACA 
ACAGAGAAAG ACAAAAGCAA AGCGACTGAT GACAACACAG CTGAGACAAA AACAGACAAT 
AAAGCTAGCA AGAAAAGCGA AAAACCAAAG GCCAAAACAA CAAAAAAGAC ATCAAAAGCA 
GAAACCCAAA CAAAAGCTAA AGCAGACAAA ACAAAAACCA CAACAGCGGA GAAAAAGAAA 
ACAAAACCAG CCAGCAAGAC AAAACCTGTA ACGACAGACA AAAAGGAGAA ACCGCCCGGC 
AAGAAAACAG CTGCAAATGA CAAGAAAACC AAACCAAAAG CCACAGAAGC GAAAGACTCC 
AAAGCTGAAA AACACAACGA TGAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p3h1 ( XM_002663819.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663865.1 Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.

Price & Availability↑

CloneID	ODa17329
Clone ID Related Accession (Same CDS sequence)	XM_002663819.4 , XM_002663819.5
Accession Version	XM_002663819.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	prolyl 3-hydroxylase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663819.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGACCT CACTGTGCCT CATCTCAATC CTCTCCACTG TCCACTTCAC CCGGTGTGAC 
CCTCAAATCA ATGCGGGATT TCAGCCGTAC GACCTGCTCT TCGACAACGC GGTGGAGGCG 
TATTATAAGC AGGACTGGCT GGCTGTGATC CTCAACATGG AGAAAGCGCT GCGGAATAAA 
GGAGCGCTGC GTAAAATACA GACGCACTGT CGCCTGTCCT GCGCCAATAA CACCGCTTTT 
GGGGAGCCTC TTCCCGGTTT GGGCATCCCG ATCCCAGGCT CCGGCTCTGT GGAGGATTTA 
GCGTTTTTCC AGAGGATCCT CCAACGGGCC GATTGCGTGA ATGCGTGTGA AAGCGAGAAA 
CTGGGACCGG CGACCCTGCA TAAAGTTAGT GAGAATGTTG AGCTGGAGTT TAAGAAGAGG 
ACGCCGTATA ATTACCTGCA AGTGGCCTAC TTTAAGATAA ACAAGCTTGA CAAGGCAGTG 
GCAGCAGCAA ATACATTTTT CATGGCGAAC CCCGAGCATG TGGAGATGAA ACAGAATTTA 
GATTATTACA GGATGATGGC TGGGGTGCAG GAGACCGATT TCAAGGACCT GGAGGAGAAA 
CCGCACATGG CAGAATTTTT GCAAGGCAAG ATTCATTACA GTACGGAAGA TTTCGTTCCA 
GCCGTCGAGC ATTTTGAGGC GGCGGTTGAA GAGTATTTCG GCGCTTATGA GGAATGCCGA 
GTTCTGTGTG AGGGAGCGTT CGATTATGAT GGATACAACT ACATGGAGTA CAACGCTGAT 
CTCTTCCAGT CAATGACAGA TCATTATCTT CATGTTTTGA GCTGCAAACA AAACTGTGCC 
GTTGAGCTGG CATCCACCGC TGGGAGAGAA ACACCTTTTG AAGATTTCCT GCCCTCACAC 
TTCAACTACC TGCAGTTCTC ATACTATAAC ATGGAGAAAT TCGAGCAGGC CATTGAGTGT 
GCTAAAACAT ACCTGCTCTT CCACCCTGAG GATGCAGTCA TGGCTCAGAA CATTGCGTAT 
TACTCCGCTG TGCTGGGAGA CGAAAAAGCC GTCAACATCA CGGAAAGAGA GGTGGTTAAA 
CAGCATATAA AGAGATCTCT GCTGGAAAAA GAGCTCCTTT ACTACGGCTA TGAGATGTTT 
GGTAAAGCGT TTGTTGATCC AGACACATGG ACACCTGAGG AAGTCATGCC CAAGAAACTA 
AGAGACAAAC AGAAGGTGGA CAAAGAAACA GCGGCGAGGA TTACTGAGGA GATCGGTAAC 
CTGATGAAAG AGATAGAGAC ACTGGTGGAA GAAAAGAGTA AAGAGTCATC AGACATCGTC 
AAGGTTGTGA AAGATGAAAT CAAACCAGCT GCTGCTCCAG ATACTGCCAG CAAACTCCCA 
TCTGATGACA TCACCATAAC CATGACCTCC GAGGTTCTGA GCGGTTCACA TCGAGTTGTC 
CTAGATGGAG TAACCACATC TGATGAGTGT CAGGAACTTC ATCAAATCAC CACTACAGCA 
GCTCTTCAAG ATGCTTTTAA AGCAGTGCCA TCCCCTCATT CAGCGAGTGA GATGTTTCAG 
GACGTCCCAG TTCTCAAAGC TCTGCAGGAA GGTCTAATTC CTACAAAAAG TGCACATTTG 
CTGTCCAATC TAGGTGAGAA GGTCCGTAAG GTTCTTGAAT CCTACTTCGC TCTGGAATCA 
ACGCTCTATT TCTCCAGCTC AAACCTGGTC TGCAGATCTG CCACTGAAAA ACAGGAGGCC 
AGTGCAGACT GTCTGCTTAT TTCCGAGCTC AACGAATGTA TCAAAGTGCT GTCAACAAAC 
ACCAATCAAG GCTACAGTGC CATTTTGTAC CTCAATGATG ATTTCGAAGG AGGAGATTTT 
ATCTTCACCG AGTCGGATGG CAAAGCTGTC TCTGCCGCAG TGAAGCCTCA GTGTGGTCGA 
GTGGTGGGCT TCAGAGCCGG ACAGGAGAAT CTTCATGGGG TCTCAGCGGT AACTAAAGGC 
CAGAGGTGTG CTGTAGTGCT TCACTTCACT CTGGATCCTC AGCACAGTCA GGAGGAGCGT 
TCACAGACTG AAGAACTTCT GAAAAAGCTT TCGAGCCCAG TTAAAGTAGA CATCCAGAAA 
ACGGCGACAG ACAGTCAAGA GTACCCCTCC AAACCCGAAG GAACAATTCC TGCCGAAACA 
ACAGAGAAAG ACAAAAGCAA AGCGACTGAT GACAACACAG CTGAGACAAA AACAGACAAT 
AAAGCTAGCA AGAAAAGCGA AAAACCAAAG GCCAAAACAA CAAAAAAGAC ATCAAAAGCA 
GAAACCCAAA CAAAAGCTAA AGCAGACAAA ACAAAAACCA CAACAGCGGA GAAAAAGAAA 
ACAAAACCAG CCAGCAAGAC AAAACCTGTA ACGACAGACA AAAAGGAGAA ACCGCCCGGC 
AAGAAAACAG CTGCAAATGA CAAGAAAACC AAACCAAAAG CCACAGAAGC GAAAGACTCC 
AAAGCTGAAA AACACAACGA TGAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663865.1
CDS	82..2571
Translation	MWTSLCLISILSTVHFTRCDPQINAGFQPYDLLFDNAVEAYYKQDWLAVILNMEKALRNKGALRKIQTHCRLSCANNTAFGEPLPGLGIPIPGSGSVEDLAFFQRILQRADCVNACESEKLGPATLHKVSENVELEFKKRTPYNYLQVAYFKINKLDKAVAAANTFFMANPEHVEMKQNLDYYRMMAGVQETDFKDLEEKPHMAEFLQGKIHYSTEDFVPAVEHFEAAVEEYFGAYEECRVLCEGAFDYDGYNYMEYNADLFQSMTDHYLHVLSCKQNCAVELASTAGRETPFEDFLPSHFNYLQFSYYNMEKFEQAIECAKTYLLFHPEDAVMAQNIAYYSAVLGDEKAVNITEREVVKQHIKRSLLEKELLYYGYEMFGKAFVDPDTWTPEEVMPKKLRDKQKVDKETAARITEEIGNLMKEIETLVEEKSKESSDIVKVVKDEIKPAAAPDTASKLPSDDITITMTSEVLSGSHRVVLDGVTTSDECQELHQITTTAALQDAFKAVPSPHSASEMFQDVPVLKALQEGLIPTKSAHLLSNLGEKVRKVLESYFALESTLYFSSSNLVCRSATEKQEASADCLLISELNECIKVLSTNTNQGYSAILYLNDDFEGGDFIFTESDGKAVSAAVKPQCGRVVGFRAGQENLHGVSAVTKGQRCAVVLHFTLDPQHSQEERSQTEELLKKLSSPVKVDIQKTATDSQEYPSKPEGTIPAETTEKDKSKATDDNTAETKTDNKASKKSEKPKAKTTKKTSKAETQTKAKADKTKTTTAEKKKTKPASKTKPVTTDKKEKPPGKKTAANDKKTKPKATEAKDSKAEKHNDEL

Target ORF information:

RefSeq Version	XM_002663819.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio prolyl 3-hydroxylase 1 (p3h1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663819.5

ORF Insert Sequence:

ATGTGGACCT CACTGTGCCT CATCTCAATC CTCTCCACTG TCCACTTCAC CCGGTGTGAC 
CCTCAAATCA ATGCGGGATT TCAGCCGTAC GACCTGCTCT TCGACAACGC GGTGGAGGCG 
TATTATAAGC AGGACTGGCT GGCTGTGATC CTCAACATGG AGAAAGCGCT GCGGAATAAA 
GGAGCGCTGC GTAAAATACA GACGCACTGT CGCCTGTCCT GCGCCAATAA CACCGCTTTT 
GGGGAGCCTC TTCCCGGTTT GGGCATCCCG ATCCCAGGCT CCGGCTCTGT GGAGGATTTA 
GCGTTTTTCC AGAGGATCCT CCAACGGGCC GATTGCGTGA ATGCGTGTGA AAGCGAGAAA 
CTGGGACCGG CGACCCTGCA TAAAGTTAGT GAGAATGTTG AGCTGGAGTT TAAGAAGAGG 
ACGCCGTATA ATTACCTGCA AGTGGCCTAC TTTAAGATAA ACAAGCTTGA CAAGGCAGTG 
GCAGCAGCAA ATACATTTTT CATGGCGAAC CCCGAGCATG TGGAGATGAA ACAGAATTTA 
GATTATTACA GGATGATGGC TGGGGTGCAG GAGACCGATT TCAAGGACCT GGAGGAGAAA 
CCGCACATGG CAGAATTTTT GCAAGGCAAG ATTCATTACA GTACGGAAGA TTTCGTTCCA 
GCCGTCGAGC ATTTTGAGGC GGCGGTTGAA GAGTATTTCG GCGCTTATGA GGAATGCCGA 
GTTCTGTGTG AGGGAGCGTT CGATTATGAT GGATACAACT ACATGGAGTA CAACGCTGAT 
CTCTTCCAGT CAATGACAGA TCATTATCTT CATGTTTTGA GCTGCAAACA AAACTGTGCC 
GTTGAGCTGG CATCCACCGC TGGGAGAGAA ACACCTTTTG AAGATTTCCT GCCCTCACAC 
TTCAACTACC TGCAGTTCTC ATACTATAAC ATGGAGAAAT TCGAGCAGGC CATTGAGTGT 
GCTAAAACAT ACCTGCTCTT CCACCCTGAG GATGCAGTCA TGGCTCAGAA CATTGCGTAT 
TACTCCGCTG TGCTGGGAGA CGAAAAAGCC GTCAACATCA CGGAAAGAGA GGTGGTTAAA 
CAGCATATAA AGAGATCTCT GCTGGAAAAA GAGCTCCTTT ACTACGGCTA TGAGATGTTT 
GGTAAAGCGT TTGTTGATCC AGACACATGG ACACCTGAGG AAGTCATGCC CAAGAAACTA 
AGAGACAAAC AGAAGGTGGA CAAAGAAACA GCGGCGAGGA TTACTGAGGA GATCGGTAAC 
CTGATGAAAG AGATAGAGAC ACTGGTGGAA GAAAAGAGTA AAGAGTCATC AGACATCGTC 
AAGGTTGTGA AAGATGAAAT CAAACCAGCT GCTGCTCCAG ATACTGCCAG CAAACTCCCA 
TCTGATGACA TCACCATAAC CATGACCTCC GAGGTTCTGA GCGGTTCACA TCGAGTTGTC 
CTAGATGGAG TAACCACATC TGATGAGTGT CAGGAACTTC ATCAAATCAC CACTACAGCA 
GCTCTTCAAG ATGCTTTTAA AGCAGTGCCA TCCCCTCATT CAGCGAGTGA GATGTTTCAG 
GACGTCCCAG TTCTCAAAGC TCTGCAGGAA GGTCTAATTC CTACAAAAAG TGCACATTTG 
CTGTCCAATC TAGGTGAGAA GGTCCGTAAG GTTCTTGAAT CCTACTTCGC TCTGGAATCA 
ACGCTCTATT TCTCCAGCTC AAACCTGGTC TGCAGATCTG CCACTGAAAA ACAGGAGGCC 
AGTGCAGACT GTCTGCTTAT TTCCGAGCTC AACGAATGTA TCAAAGTGCT GTCAACAAAC 
ACCAATCAAG GCTACAGTGC CATTTTGTAC CTCAATGATG ATTTCGAAGG AGGAGATTTT 
ATCTTCACCG AGTCGGATGG CAAAGCTGTC TCTGCCGCAG TGAAGCCTCA GTGTGGTCGA 
GTGGTGGGCT TCAGAGCCGG ACAGGAGAAT CTTCATGGGG TCTCAGCGGT AACTAAAGGC 
CAGAGGTGTG CTGTAGTGCT TCACTTCACT CTGGATCCTC AGCACAGTCA GGAGGAGCGT 
TCACAGACTG AAGAACTTCT GAAAAAGCTT TCGAGCCCAG TTAAAGTAGA CATCCAGAAA 
ACGGCGACAG ACAGTCAAGA GTACCCCTCC AAACCCGAAG GAACAATTCC TGCCGAAACA 
ACAGAGAAAG ACAAAAGCAA AGCGACTGAT GACAACACAG CTGAGACAAA AACAGACAAT 
AAAGCTAGCA AGAAAAGCGA AAAACCAAAG GCCAAAACAA CAAAAAAGAC ATCAAAAGCA 
GAAACCCAAA CAAAAGCTAA AGCAGACAAA ACAAAAACCA CAACAGCGGA GAAAAAGAAA 
ACAAAACCAG CCAGCAAGAC AAAACCTGTA ACGACAGACA AAAAGGAGAA ACCGCCCGGC 
AAGAAAACAG CTGCAAATGA CAAGAAAACC AAACCAAAAG CCACAGAAGC GAAAGACTCC 
AAAGCTGAAA AACACAACGA TGAGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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p3h1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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